definition of hypothesis in plants

Hypothesis n., plural: hypotheses [/haɪˈpɑːθəsɪs/] Definition: Testable scientific prediction

Table of Contents

What Is Hypothesis?

A scientific hypothesis is a foundational element of the scientific method . It’s a testable statement proposing a potential explanation for natural phenomena. The term hypothesis means “little theory” . A hypothesis is a short statement that can be tested and gives a possible reason for a phenomenon or a possible link between two variables . In the setting of scientific research, a hypothesis is a tentative explanation or statement that can be proven wrong and is used to guide experiments and empirical research.

It is an important part of the scientific method because it gives a basis for planning tests, gathering data, and judging evidence to see if it is true and could help us understand how natural things work. Several hypotheses can be tested in the real world, and the results of careful and systematic observation and analysis can be used to support, reject, or improve them.

Researchers and scientists often use the word hypothesis to refer to this educated guess . These hypotheses are firmly established based on scientific principles and the rigorous testing of new technology and experiments .

For example, in astrophysics, the Big Bang Theory is a working hypothesis that explains the origins of the universe and considers it as a natural phenomenon. It is among the most prominent scientific hypotheses in the field.

“The scientific method: steps, terms, and examples” by Scishow:

Biology definition: A hypothesis  is a supposition or tentative explanation for (a group of) phenomena, (a set of) facts, or a scientific inquiry that may be tested, verified or answered by further investigation or methodological experiment. It is like a scientific guess . It’s an idea or prediction that scientists make before they do experiments. They use it to guess what might happen and then test it to see if they were right. It’s like a smart guess that helps them learn new things. A scientific hypothesis that has been verified through scientific experiment and research may well be considered a scientific theory .

Etymology: The word “hypothesis” comes from the Greek word “hupothesis,” which means “a basis” or “a supposition.” It combines “hupo” (under) and “thesis” (placing). Synonym:   proposition; assumption; conjecture; postulate Compare:   theory See also: null hypothesis

Characteristics Of Hypothesis

A useful hypothesis must have the following qualities:

• It should never be written as a question.
• You should be able to test it in the real world to see if it’s right or wrong.
• It needs to be clear and exact.
• It should list the factors that will be used to figure out the relationship.
• It should only talk about one thing. You can make a theory in either a descriptive or form of relationship.
• It shouldn’t go against any natural rule that everyone knows is true. Verification will be done well with the tools and methods that are available.
• It should be written in as simple a way as possible so that everyone can understand it.
• It must explain what happened to make an answer necessary.
• It should be testable in a fair amount of time.
• It shouldn’t say different things.

Sources Of Hypothesis

Sources of hypothesis are:

• Patterns of similarity between the phenomenon under investigation and existing hypotheses.
• Insights derived from prior research, concurrent observations, and insights from opposing perspectives.
• The formulations are derived from accepted scientific theories and proposed by researchers.
• In research, it’s essential to consider hypothesis as different subject areas may require various hypotheses (plural form of hypothesis). Researchers also establish a significance level to determine the strength of evidence supporting a hypothesis.
• Individual cognitive processes also contribute to the formation of hypotheses.

One hypothesis is a tentative explanation for an observation or phenomenon. It is based on prior knowledge and understanding of the world, and it can be tested by gathering and analyzing data. Observed facts are the data that are collected to test a hypothesis. They can support or refute the hypothesis.

For example, the hypothesis that “eating more fruits and vegetables will improve your health” can be tested by gathering data on the health of people who eat different amounts of fruits and vegetables. If the people who eat more fruits and vegetables are healthier than those who eat less fruits and vegetables, then the hypothesis is supported.

Hypotheses are essential for scientific inquiry. They help scientists to focus their research, to design experiments, and to interpret their results. They are also essential for the development of scientific theories.

Types Of Hypothesis

In research, you typically encounter two types of hypothesis: the alternative hypothesis (which proposes a relationship between variables) and the null hypothesis (which suggests no relationship).

Simple Hypothesis

It illustrates the association between one dependent variable and one independent variable. For instance, if you consume more vegetables, you will lose weight more quickly. Here, increasing vegetable consumption is the independent variable, while weight loss is the dependent variable.

Complex Hypothesis

It exhibits the relationship between at least two dependent variables and at least two independent variables. Eating more vegetables and fruits results in weight loss, radiant skin, and a decreased risk of numerous diseases, including heart disease.

Directional Hypothesis

It shows that a researcher wants to reach a certain goal. The way the factors are related can also tell us about their nature. For example, four-year-old children who eat well over a time of five years have a higher IQ than children who don’t eat well. This shows what happened and how it happened.

Non-directional Hypothesis

When there is no theory involved, it is used. It is a statement that there is a connection between two variables, but it doesn’t say what that relationship is or which way it goes.

Null Hypothesis

It says something that goes against the theory. It’s a statement that says something is not true, and there is no link between the independent and dependent factors. “H 0 ” represents the null hypothesis.

Associative and Causal Hypothesis

When a change in one variable causes a change in the other variable, this is called the associative hypothesis . The causal hypothesis, on the other hand, says that there is a cause-and-effect relationship between two or more factors.

Examples Of Hypothesis

Examples of simple hypotheses:

• Students who consume breakfast before taking a math test will have a better overall performance than students who do not consume breakfast.
• Students who experience test anxiety before an English examination will get lower scores than students who do not experience test anxiety.
• Motorists who talk on the phone while driving will be more likely to make errors on a driving course than those who do not talk on the phone, is a statement that suggests that drivers who talk on the phone while driving are more likely to make mistakes.

Examples of a complex hypothesis:

• Individuals who consume a lot of sugar and don’t get much exercise are at an increased risk of developing depression.
• Younger people who are routinely exposed to green, outdoor areas have better subjective well-being than older adults who have limited exposure to green spaces, according to a new study.
• Increased levels of air pollution led to higher rates of respiratory illnesses, which in turn resulted in increased costs for healthcare for the affected communities.

Examples of Directional Hypothesis:

• The crop yield will go up a lot if the amount of fertilizer is increased.
• Patients who have surgery and are exposed to more stress will need more time to get better.
• Increasing the frequency of brand advertising on social media will lead to a significant increase in brand awareness among the target audience.

Examples of Non-Directional Hypothesis (or Two-Tailed Hypothesis):

• The test scores of two groups of students are very different from each other.
• There is a link between gender and being happy at work.
• There is a correlation between the amount of caffeine an individual consumes and the speed with which they react.

Examples of a null hypothesis:

• Children who receive a new reading intervention will have scores that are different than students who do not receive the intervention.
• The results of a memory recall test will not reveal any significant gap in performance between children and adults.
• There is not a significant relationship between the number of hours spent playing video games and academic performance.

Examples of Associative Hypothesis:

• There is a link between how many hours you spend studying and how well you do in school.
• Drinking sugary drinks is bad for your health as a whole.
• There is an association between socioeconomic status and access to quality healthcare services in urban neighborhoods.

Functions Of Hypothesis

The research issue can be understood better with the help of a hypothesis, which is why developing one is crucial. The following are some of the specific roles that a hypothesis plays: (Rashid, Apr 20, 2022)

• A hypothesis gives a study a point of concentration. It enlightens us as to the specific characteristics of a study subject we need to look into.
• It instructs us on what data to acquire as well as what data we should not collect, giving the study a focal point .
• The development of a hypothesis improves objectivity since it enables the establishment of a focal point.
• A hypothesis makes it possible for us to contribute to the development of the theory. Because of this, we are in a position to definitively determine what is true and what is untrue .

How will Hypothesis help in the Scientific Method?

• The scientific method begins with observation and inquiry about the natural world when formulating research questions. Researchers can refine their observations and queries into specific, testable research questions with the aid of hypothesis. They provide an investigation with a focused starting point.
• Hypothesis generate specific predictions regarding the expected outcomes of experiments or observations. These forecasts are founded on the researcher’s current knowledge of the subject. They elucidate what researchers anticipate observing if the hypothesis is true.
• Hypothesis direct the design of experiments and data collection techniques. Researchers can use them to determine which variables to measure or manipulate, which data to obtain, and how to conduct systematic and controlled research.
• Following the formulation of a hypothesis and the design of an experiment, researchers collect data through observation, measurement, or experimentation. The collected data is used to verify the hypothesis’s predictions.
• Hypothesis establish the criteria for evaluating experiment results. The observed data are compared to the predictions generated by the hypothesis. This analysis helps determine whether empirical evidence supports or refutes the hypothesis.
• The results of experiments or observations are used to derive conclusions regarding the hypothesis. If the data support the predictions, then the hypothesis is supported. If this is not the case, the hypothesis may be revised or rejected, leading to the formulation of new queries and hypothesis.
• The scientific approach is iterative, resulting in new hypothesis and research issues from previous trials. This cycle of hypothesis generation, testing, and refining drives scientific progress.

Importance Of Hypothesis

• Hypothesis are testable statements that enable scientists to determine if their predictions are accurate. This assessment is essential to the scientific method, which is based on empirical evidence.
• Hypothesis serve as the foundation for designing experiments or data collection techniques. They can be used by researchers to develop protocols and procedures that will produce meaningful results.
• Hypothesis hold scientists accountable for their assertions. They establish expectations for what the research should reveal and enable others to assess the validity of the findings.
• Hypothesis aid in identifying the most important variables of a study. The variables can then be measured, manipulated, or analyzed to determine their relationships.
• Hypothesis assist researchers in allocating their resources efficiently. They ensure that time, money, and effort are spent investigating specific concerns, as opposed to exploring random concepts.
• Testing hypothesis contribute to the scientific body of knowledge. Whether or not a hypothesis is supported, the results contribute to our understanding of a phenomenon.
• Hypothesis can result in the creation of theories. When supported by substantive evidence, hypothesis can serve as the foundation for larger theoretical frameworks that explain complex phenomena.
• Beyond scientific research, hypothesis play a role in the solution of problems in a variety of domains. They enable professionals to make educated assumptions about the causes of problems and to devise solutions.

Research Hypotheses: Did you know that a hypothesis refers to an educated guess or prediction about the outcome of a research study?

It’s like a roadmap guiding researchers towards their destination of knowledge. Just like a compass points north, a well-crafted hypothesis points the way to valuable discoveries in the world of science and inquiry.

Choose the best answer. 

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Further reading.

• RNA-DNA World Hypothesis
• BYJU’S. (2023). Hypothesis. Retrieved 01 Septermber 2023, from https://byjus.com/physics/hypothesis/#sources-of-hypothesis
• Collegedunia. (2023). Hypothesis. Retrieved 1 September 2023, from https://collegedunia.com/exams/hypothesis-science-articleid-7026#d
• Hussain, D. J. (2022). Hypothesis. Retrieved 01 September 2023, from https://mmhapu.ac.in/doc/eContent/Management/JamesHusain/Research%20Hypothesis%20-Meaning,%20Nature%20&%20Importance-Characteristics%20of%20Good%20%20Hypothesis%20Sem2.pdf
• Media, D. (2023). Hypothesis in the Scientific Method. Retrieved 01 September 2023, from https://www.verywellmind.com/what-is-a-hypothesis-2795239#toc-hypotheses-examples
• Rashid, M. H. A. (Apr 20, 2022). Research Methodology. Retrieved 01 September 2023, from https://limbd.org/hypothesis-definitions-functions-characteristics-types-errors-the-process-of-testing-a-hypothesis-hypotheses-in-qualitative-research/#:~:text=Functions%20of%20a%20Hypothesis%3A&text=Specifically%2C%20a%20hypothesis%20serves%20the,providing%20focus%20to%20the%20study.

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Last updated on September 8th, 2023

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What is a scientific hypothesis?

It's the initial building block in the scientific method.

Hypothesis basics

What makes a hypothesis testable.

• Types of hypotheses
• Hypothesis versus theory

Additional resources

Bibliography.

A scientific hypothesis is a tentative, testable explanation for a phenomenon in the natural world. It's the initial building block in the scientific method . Many describe it as an "educated guess" based on prior knowledge and observation. While this is true, a hypothesis is more informed than a guess. While an "educated guess" suggests a random prediction based on a person's expertise, developing a hypothesis requires active observation and background research. 

The basic idea of a hypothesis is that there is no predetermined outcome. For a solution to be termed a scientific hypothesis, it has to be an idea that can be supported or refuted through carefully crafted experimentation or observation. This concept, called falsifiability and testability, was advanced in the mid-20th century by Austrian-British philosopher Karl Popper in his famous book "The Logic of Scientific Discovery" (Routledge, 1959).

A key function of a hypothesis is to derive predictions about the results of future experiments and then perform those experiments to see whether they support the predictions.

A hypothesis is usually written in the form of an if-then statement, which gives a possibility (if) and explains what may happen because of the possibility (then). The statement could also include "may," according to California State University, Bakersfield .

Here are some examples of hypothesis statements:

• If garlic repels fleas, then a dog that is given garlic every day will not get fleas.
• If sugar causes cavities, then people who eat a lot of candy may be more prone to cavities.
• If ultraviolet light can damage the eyes, then maybe this light can cause blindness.

A useful hypothesis should be testable and falsifiable. That means that it should be possible to prove it wrong. A theory that can't be proved wrong is nonscientific, according to Karl Popper's 1963 book " Conjectures and Refutations ."

An example of an untestable statement is, "Dogs are better than cats." That's because the definition of "better" is vague and subjective. However, an untestable statement can be reworded to make it testable. For example, the previous statement could be changed to this: "Owning a dog is associated with higher levels of physical fitness than owning a cat." With this statement, the researcher can take measures of physical fitness from dog and cat owners and compare the two.

Types of scientific hypotheses

In an experiment, researchers generally state their hypotheses in two ways. The null hypothesis predicts that there will be no relationship between the variables tested, or no difference between the experimental groups. The alternative hypothesis predicts the opposite: that there will be a difference between the experimental groups. This is usually the hypothesis scientists are most interested in, according to the University of Miami .

For example, a null hypothesis might state, "There will be no difference in the rate of muscle growth between people who take a protein supplement and people who don't." The alternative hypothesis would state, "There will be a difference in the rate of muscle growth between people who take a protein supplement and people who don't."

If the results of the experiment show a relationship between the variables, then the null hypothesis has been rejected in favor of the alternative hypothesis, according to the book " Research Methods in Psychology " (​​BCcampus, 2015). 

There are other ways to describe an alternative hypothesis. The alternative hypothesis above does not specify a direction of the effect, only that there will be a difference between the two groups. That type of prediction is called a two-tailed hypothesis. If a hypothesis specifies a certain direction — for example, that people who take a protein supplement will gain more muscle than people who don't — it is called a one-tailed hypothesis, according to William M. K. Trochim , a professor of Policy Analysis and Management at Cornell University.

Sometimes, errors take place during an experiment. These errors can happen in one of two ways. A type I error is when the null hypothesis is rejected when it is true. This is also known as a false positive. A type II error occurs when the null hypothesis is not rejected when it is false. This is also known as a false negative, according to the University of California, Berkeley . 

A hypothesis can be rejected or modified, but it can never be proved correct 100% of the time. For example, a scientist can form a hypothesis stating that if a certain type of tomato has a gene for red pigment, that type of tomato will be red. During research, the scientist then finds that each tomato of this type is red. Though the findings confirm the hypothesis, there may be a tomato of that type somewhere in the world that isn't red. Thus, the hypothesis is true, but it may not be true 100% of the time.

Scientific theory vs. scientific hypothesis

The best hypotheses are simple. They deal with a relatively narrow set of phenomena. But theories are broader; they generally combine multiple hypotheses into a general explanation for a wide range of phenomena, according to the University of California, Berkeley . For example, a hypothesis might state, "If animals adapt to suit their environments, then birds that live on islands with lots of seeds to eat will have differently shaped beaks than birds that live on islands with lots of insects to eat." After testing many hypotheses like these, Charles Darwin formulated an overarching theory: the theory of evolution by natural selection.

"Theories are the ways that we make sense of what we observe in the natural world," Tanner said. "Theories are structures of ideas that explain and interpret facts." 

• Read more about writing a hypothesis, from the American Medical Writers Association.
• Find out why a hypothesis isn't always necessary in science, from The American Biology Teacher.
• Learn about null and alternative hypotheses, from Prof. Essa on YouTube .

Encyclopedia Britannica. Scientific Hypothesis. Jan. 13, 2022. https://www.britannica.com/science/scientific-hypothesis

Karl Popper, "The Logic of Scientific Discovery," Routledge, 1959.

California State University, Bakersfield, "Formatting a testable hypothesis." https://www.csub.edu/~ddodenhoff/Bio100/Bio100sp04/formattingahypothesis.htm  

Karl Popper, "Conjectures and Refutations," Routledge, 1963.

Price, P., Jhangiani, R., & Chiang, I., "Research Methods of Psychology — 2nd Canadian Edition," BCcampus, 2015.‌

University of Miami, "The Scientific Method" http://www.bio.miami.edu/dana/161/evolution/161app1_scimethod.pdf  

William M.K. Trochim, "Research Methods Knowledge Base," https://conjointly.com/kb/hypotheses-explained/  

University of California, Berkeley, "Multiple Hypothesis Testing and False Discovery Rate" https://www.stat.berkeley.edu/~hhuang/STAT141/Lecture-FDR.pdf  

University of California, Berkeley, "Science at multiple levels" https://undsci.berkeley.edu/article/0_0_0/howscienceworks_19

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What Is A Research (Scientific) Hypothesis? A plain-language explainer + examples

By:  Derek Jansen (MBA)  | Reviewed By: Dr Eunice Rautenbach | June 2020

If you’re new to the world of research, or it’s your first time writing a dissertation or thesis, you’re probably noticing that the words “research hypothesis” and “scientific hypothesis” are used quite a bit, and you’re wondering what they mean in a research context .

“Hypothesis” is one of those words that people use loosely, thinking they understand what it means. However, it has a very specific meaning within academic research. So, it’s important to understand the exact meaning before you start hypothesizing. 

Research Hypothesis 101

• What is a hypothesis ?
• What is a research hypothesis (scientific hypothesis)?
• Requirements for a research hypothesis
• Definition of a research hypothesis
• The null hypothesis

What is a hypothesis?

Let’s start with the general definition of a hypothesis (not a research hypothesis or scientific hypothesis), according to the Cambridge Dictionary:

Hypothesis: an idea or explanation for something that is based on known facts but has not yet been proved.

In other words, it’s a statement that provides an explanation for why or how something works, based on facts (or some reasonable assumptions), but that has not yet been specifically tested . For example, a hypothesis might look something like this:

Hypothesis: sleep impacts academic performance.

This statement predicts that academic performance will be influenced by the amount and/or quality of sleep a student engages in – sounds reasonable, right? It’s based on reasonable assumptions , underpinned by what we currently know about sleep and health (from the existing literature). So, loosely speaking, we could call it a hypothesis, at least by the dictionary definition.

But that’s not good enough…

Unfortunately, that’s not quite sophisticated enough to describe a research hypothesis (also sometimes called a scientific hypothesis), and it wouldn’t be acceptable in a dissertation, thesis or research paper . In the world of academic research, a statement needs a few more criteria to constitute a true research hypothesis .

What is a research hypothesis?

A research hypothesis (also called a scientific hypothesis) is a statement about the expected outcome of a study (for example, a dissertation or thesis). To constitute a quality hypothesis, the statement needs to have three attributes – specificity , clarity and testability .

Let’s take a look at these more closely.

Need a helping hand?

Hypothesis Essential #1: Specificity & Clarity

A good research hypothesis needs to be extremely clear and articulate about both what’ s being assessed (who or what variables are involved ) and the expected outcome (for example, a difference between groups, a relationship between variables, etc.).

Let’s stick with our sleepy students example and look at how this statement could be more specific and clear.

Hypothesis: Students who sleep at least 8 hours per night will, on average, achieve higher grades in standardised tests than students who sleep less than 8 hours a night.

As you can see, the statement is very specific as it identifies the variables involved (sleep hours and test grades), the parties involved (two groups of students), as well as the predicted relationship type (a positive relationship). There’s no ambiguity or uncertainty about who or what is involved in the statement, and the expected outcome is clear.

Contrast that to the original hypothesis we looked at – “Sleep impacts academic performance” – and you can see the difference. “Sleep” and “academic performance” are both comparatively vague , and there’s no indication of what the expected relationship direction is (more sleep or less sleep). As you can see, specificity and clarity are key.

Hypothesis Essential #2: Testability (Provability)

A statement must be testable to qualify as a research hypothesis. In other words, there needs to be a way to prove (or disprove) the statement. If it’s not testable, it’s not a hypothesis – simple as that.

For example, consider the hypothesis we mentioned earlier:

Hypothesis: Students who sleep at least 8 hours per night will, on average, achieve higher grades in standardised tests than students who sleep less than 8 hours a night.  

We could test this statement by undertaking a quantitative study involving two groups of students, one that gets 8 or more hours of sleep per night for a fixed period, and one that gets less. We could then compare the standardised test results for both groups to see if there’s a statistically significant difference. 

Again, if you compare this to the original hypothesis we looked at – “Sleep impacts academic performance” – you can see that it would be quite difficult to test that statement, primarily because it isn’t specific enough. How much sleep? By who? What type of academic performance?

So, remember the mantra – if you can’t test it, it’s not a hypothesis 🙂

Defining A Research Hypothesis

You’re still with us? Great! Let’s recap and pin down a clear definition of a hypothesis.

A research hypothesis (or scientific hypothesis) is a statement about an expected relationship between variables, or explanation of an occurrence, that is clear, specific and testable.

So, when you write up hypotheses for your dissertation or thesis, make sure that they meet all these criteria. If you do, you’ll not only have rock-solid hypotheses but you’ll also ensure a clear focus for your entire research project.

What about the null hypothesis?

You may have also heard the terms null hypothesis , alternative hypothesis, or H-zero thrown around. At a simple level, the null hypothesis is the counter-proposal to the original hypothesis.

For example, if the hypothesis predicts that there is a relationship between two variables (for example, sleep and academic performance), the null hypothesis would predict that there is no relationship between those variables.

At a more technical level, the null hypothesis proposes that no statistical significance exists in a set of given observations and that any differences are due to chance alone.

And there you have it – hypotheses in a nutshell. 

If you have any questions, be sure to leave a comment below and we’ll do our best to help you. If you need hands-on help developing and testing your hypotheses, consider our private coaching service , where we hold your hand through the research journey.

17 Comments

Very useful information. I benefit more from getting more information in this regard.

Very great insight,educative and informative. Please give meet deep critics on many research data of public international Law like human rights, environment, natural resources, law of the sea etc

In a book I read a distinction is made between null, research, and alternative hypothesis. As far as I understand, alternative and research hypotheses are the same. Can you please elaborate? Best Afshin

This is a self explanatory, easy going site. I will recommend this to my friends and colleagues.

Very good definition. How can I cite your definition in my thesis? Thank you. Is nul hypothesis compulsory in a research?

It’s a counter-proposal to be proven as a rejection

Please what is the difference between alternate hypothesis and research hypothesis?

It is a very good explanation. However, it limits hypotheses to statistically tasteable ideas. What about for qualitative researches or other researches that involve quantitative data that don’t need statistical tests?

In qualitative research, one typically uses propositions, not hypotheses.

could you please elaborate it more

I’ve benefited greatly from these notes, thank you.

This is very helpful

well articulated ideas are presented here, thank you for being reliable sources of information

Excellent. Thanks for being clear and sound about the research methodology and hypothesis (quantitative research)

I have only a simple question regarding the null hypothesis. – Is the null hypothesis (Ho) known as the reversible hypothesis of the alternative hypothesis (H1? – How to test it in academic research?

this is very important note help me much more

Hi” best wishes to you and your very nice blog” 

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• What Is Research Methodology? Simple Definition (With Examples) - Grad Coach - […] Contrasted to this, a quantitative methodology is typically used when the research aims and objectives are confirmatory in nature. For example,…

1.2 Science and Experimentation

Learning objectives

By the end of this section you will be able to:

• Describe why science is considered a discipline of philosophy.
• Summarize the four basic types of experiments.
• Apply the principles of experimental design in this course and in your daily life.

Thinking about science

The primary goal of this section is to help you think about the nature of science. You might be taking this course to fulfill an undergraduate requirement for a biology course with a lab. This course fulfills that requirement because we investigate the process behind using science as a way of learning about the natural world around us. If you’re starting down the path to becoming a plant scientist, understanding the nature of science will be essential for you in your career

Regardless of whether you’re going to pursue a career as a scientist, now is a good time reflect on the nature of science, and to understand how scientific thinking can become a strategy for resolving many issues that you confront during daily life.

Watch this video about connecting science and experimentation to real life:

Scientific inquiry

While “science” is a word commonly used in our culture, in popular use it is rarely spoken of as a philosophy. By identifying science as a philosophy we are taking an epistemic view , one focusing on how knowledge is acquired.

At its core, science is a mode of inquiry: a way of acquiring new knowledge about the world around us and a strategy for understanding the inner workings of elements in that world. Scientists believe that if we follow the principles of this philosophy we will continue to expand our knowledge about how things work in the world around us. This systematic approach is called the “scientific method.”

There are two key steps in the scientific method:

• Hypothesis building through reflective observation.
• Hypothesis testing through experimentation.

A “hypothesis” is a question or proposed explanation made on the basis of limited evidence and used as a starting point for experimentation. Experimentation is commonly equated with science—rightly so, because hypotheses evaluated on the basis of evidence generated through experiments. Experimentation, however, isn’t the whole story. Science—including the development and testing of new hypotheses—is also a creative endeavor.

Watch this video about scientific inquiry:

Scientific inquiry has generated a vast body of knowledge about the world around us. Your school science classes might have required you to memorize facts and relationships, and pay attention to detail. Sometimes such memorization leads students to believe that science is just an accumulation of facts rather than the process behind discovering all of that information.

Scientific discovery builds on what is already known. Even the most accomplished scientists initially approach a problem by learning what is already known. Armed with that information, they then apply their own creativity to form new hypotheses about something they have observed, and design experiments to test those hypotheses. They also communicate their results publicly so that others can benefit from their work and have the opportunity to challenge conclusions. In this way, science builds on itself.

The foundational knowledge you learn in science classes prepares you to develop and test hypotheses and to make new discoveries of your own. While a good memory may help you pass a science classes, you will absorb a body of knowledge more effectively when you learn how facts fit and work together in systems rather than learning through the brute force of memorization.

In this section we work from the point of view that science is a way of acquiring knowledge—a mode of inquiry—and that this mode of inquiry follows a process called the scientific method. Those who follow the philosophy of science:

• Use it to understand how the natural world works.
• Start by learning what is already known.
• Carefully observe the subjects of their scientific inquiry and look for details about form, function, and interaction with the environment.
• Develop hypotheses about the inner workings of natural phenomena not yet understood.
• Test their hypotheses by making observations, conducting experiments and collecting and evaluating evidence.
• Communicate with others about their hypotheses, experiments, and the outcomes of their studies so that others can repeat, validate, and build upon their work.

Although science is typically used to understand how the natural world works, it is also regularly applied to the development of new technologies that are based on these natural phenomena and to the solving of problems associated with the natural world.

Putting the scientific method to work

As noted, the scientific method relies on building hypotheses and then testing them through experimentation. In the lab section of this course you will develop hypotheses about the effects of various treatments on propagation success and then conduct experiments to test those hypotheses. Because experimentation is such a key component of the scientific method, we’ll spend time characterizing and examining four types of experimentation and explore whether they are part of the scientific method. While each is valuable when applied in the right circumstances, only one clearly follows each step of the scientific method to uncover new knowledge about the natural world.

Types of experiments

The types of experimentation we will cover are:

• Demonstration
• Exploration

Demonstration experiments

Demonstration experiments are a classic method used in educational settings to help students learn and understand known relationships already discovered by others. Learners will usually have had prior exposure to the relationships through preliminary observations, lectures, reading, and discussions, and will have some sense of what the experimental outcome might be.

Good demonstration experiments actively involve the learner, who manipulates the experimental materials, applies the treatments, and observes the outcomes, then gathers, analyzes, and interprets the resulting data. Poor demonstration experiments, in contrast, make learners only passive witnesses to something done by an expert at the front of the classroom.

In the plant propagation labs for this course, you will be actively engaged in demonstration experiments. Although you won’t be creating new knowledge, the knowledge will likely be new to you. The hands-on experience of conducting the experiments will help you to learn the concepts more effectively than if you only read a textbook or listened to a lecture. The techniques you learn and use in demonstration experiments often contribute to the learning experience as much as the relationships revealed at the experiment’s conclusion. Employing these techniques will help you gain an understanding of many biological functions, such as the production of adventitious roots and mechanisms for seed dispersal.

While demonstration experiments are valuable for actively learning a body of scientific knowledge previously discovered and communicated by others, the experience is specifically orchestrated for teaching and learning, not for the discovery of new information. Yet since the knowledge is new to the learner, it can still bring the joy of personal discovery and a sense of accomplishment.

In summary, demonstration experiments:

• Are designed for teaching and learning.
• Address relationships that may be new to you, but are otherwise known.
• In their best forms, actively involve the learner.
• May emphasize experimental techniques, in addition to outcomes, as part of the learning experience.
• Are not the types of experiments that are at the core of practicing science as a way to uncover new knowledge.

Evaluation experiments

Evaluation experiments are designed to help us make decisions, and to choose from a number of options. They might, for instance, help us determine the efficacy of a new treatment relative to a known treatment, or decide on further experimentation. An evaluation experiment will highlight a compound, a technique, a piece of equipment, or an organism, and will include a control and/or other alternatives.

Evaluation experiments are common in horticultural and agronomic research, where the purpose of the experiment is to identify, for example, the best cultivar , production method, pest control, fertility regime, or light intensity for growing a crop. Correct experimental design is crucial for assuring that conclusions from the experiment are meaningful and credible.

These experiments are typically used in the development of new technologies to identify the best method for the desired purpose (e.g., which pesticides are effective against the target insect, but not harmful to non-target insects). They are not used to discover new knowledge about how the world works, as they typically don’t advance our understanding of the natural world. The information from an evaluation experiment might, however, point the way to additional experimentation that does help us discover new knowledge. This is particularly true if the outcome of an evaluation experiment is unexpected or novel.

In summary, evaluation experiments:

• Are used to help in decision-making.
• Help users choose a winner or determine efficacy relative to other alternatives.
• Are commonly used when evaluating and recommending horticultural production methods.
• Can be useful in solving problems and developing technologies.
• Require proper experimental design (e.g., comparison to a control) for credibility and meaningfulness.

Exploration experiments

Some scientists specialize in observing and cataloging nature, and some aggressively search for previously unknown phenomena. In the botanical realm, such scientists study the diversity of organisms within habitats, discover new species, or are in other ways very skilled in “seeing” nature. Explorer-scientists recognize and appreciate detail and can identify the enormous diversity among plants by comparing characteristics that might be overlooked by others. They may also have the capacity to recognize possible interrelationships among organisms and with habitats, making their work particularly important to science. They might notice, for instance, that a particular species of plant is commonly found in wet areas but not in dry, or that a particular vegetable tastes sweeter when grown at higher altitudes than when grown closer to sea level. They don’t confirm the cause of these relationships, but are the first to notice them.

Explorers’ observations are essential to stimulating the development of sound, testable hypotheses. The possible relationships they propose must be tested to determine whether those relationships actually exist, or are artifacts of other effects. Explorers help develop hypotheses, but the work of exploration, cataloging, and seeing possible relationships don’t prove or disprove the hypotheses or necessarily generate new knowledge about relationships. The work does, however, result in new information about the existence of the object or phenomenon itself. An exception is exploration done to test a hypothesis, such as a mission to test the hypothesis that a particular type of ecosystem is required for reproduction of a particular plant species.

Scientists must resist jumping to conclusions based on exploration and observation alone. If you see two people together many times, for example, you might conclude that they are a romantic couple, when in fact they are brother and sister. Relationships hypothesized as a result of exploration and observation must be experimentally tested before they are accepted or rejected.

Exploration experiments uncover new things, many of which can be exciting and eventually change our view of the world. While one of their greatest values is that they lead to the development of new and stronger hypotheses about how the world works, they go so far as to test those hypotheses or fully engage in the cycle of knowledge generation associated with the scientific method. Additional experiments based on this new information are required to put this new information in context and to advance our understanding of how the natural world works.

In summary then, exploration experiments:

• Focus on detailed observation of organisms and habitats.
• Increase our knowledge of the natural world.
• Identify potential relationships that need to be tested.
• Are essential to sound and testable hypothesis-building.

Discovery experiments

Discovery experiments are central to the use of the scientific method in tasks ranging from problem solving to the discovery of new knowledge. They focus on uncovering new relationships and solving problems, follow the scientific method, test hypotheses and their predicted outcomes, and utilize a careful design in order to maintain meaningfulness and credibility.

The similarity between the scientific method and Kolb’s Experiential Learning Cycle is not an accident. The scientific method is a practical strategy based on how we sense and experience the world around us and used to solve problems encountered during those experiences.

The diagram above illustrates a combination of the scientific method and Kolb’s four-step experiential learning, describing a cyclic process for solving problems that can be applied to disciplines as diverse as molecular biology, global warming, and even appliance repair. While you might initially think that appliance repair doesn’t belong in that list, the difference is one of application, not method. Though far removed from the esoteric scientific discoveries we associate with scientific method, appliance repair follows the same steps. Appliances are often, and quite literally, boxes, where you don’t know what is going on inside. But what’s going on inside is knowable, and through that knowledge comes repair.

The learning/problem solving/scientific process could theoretically start anywhere in Kolb’s cycle. But it will likely start with a problem that needs to be solved, something you don’t understand but would like to know more about. You become aware that there is a problem or that you lack understanding because you have an experience where you observe something and then step back and said, “I wonder how that works,” or perhaps, “why is that broken?” Through observation you develop a sufficiently adequate description of the problem to start doing some research on what is already known.

With a good description of the problem in hand, you can begin to review what is known through the work of others, and think about what might be going on in your situation and how your new understanding can be applied to the problem. This is “reflective observation.” It isn’t just sitting back and thinking in a vacuum. You need raw material for your mind to work on, and that only comes through the tough task of gathering and engaging with the background information. There is a very important quiet phase in this process when you let your mind assemble and sort through ideas until alternatives begin to emerge that might lead to a solution. Talking with others and sharing ideas is an important part of this quiet phase.

Sometimes the alternatives are no- brainers (blown fuse?), and sometimes they’re more creative (residue from the wrong detergent gunking up the water level sensor?). Regardless of their simplicity or complexity, these become hypotheses that need to be tested. The hypothesis-building stage includes both a statement of how something works or why it isn’t working, and predictions about what might happen if the hypothesis is true. In appliance repair, for example, the prediction will likely be that the appliance will function normally. In horticultural molecular biology, it might be that you will see accumulation of a particular type of fatty acid in the cotyledons .

You put the hypothesis to the test by designing an experiment that assesses whether your predictions were right. If the outcome doesn’t match your prediction, you reject the hypothesis (the fuse was ok, so that wasn’t the problem). If the outcome does match your prediction, you tentatively accept the hypothesis pending further observation (when the fuse was replaced the washing machine worked again, so it might have been a blown fuse, but on the other hand maybe it was just because the motor had time to cool down). As with evaluation experimentation, experimental design is important in assuring that the conclusions from the experiment are meaningful and credible.

Experimentation leads to new experiences and an incremental increase in knowledge, and then the cycle begins again.

In summary, discovery experiments:

• Focus on uncovering new relationships and solving problems.
• Follow scientific method.
• Test hypotheses and their predicted outcomes.
• Utilize a careful design in order to maintain meaningfulness and credibility.

Of the four types of experiments, only the discovery experiments are core to the process of science in the narrow sense of being a way of acquiring new knowledge. The other three types of experimentation are still important; demonstration and evaluation experiments are valuable for learning and decision-making and for technology development, and exploration experiments are essential for developing testable hypotheses. But discovery experiments are core to science.

Remember: the methodology of effective washing machine repair, when applied to what is unknown about the physical world, is the methodology of science. It’s not esoteric; it’s good appliance repair.

You might argue that, when applied to a broken washing machine, a discovery experiment results in knowledge that is probably already known by those skilled in appliance repair, so it isn’t really new knowledge about how the world works. That’s a fair criticism. Use of the scientific method can result in new knowledge about how the world works, but whether it uncovers new knowledge depends on the object of experimentation.

Review questions

Experimental design

The methods for designing experiments are carefully studied and often discipline-specific. Methods used in molecular biology, for instance, will be somewhat different from those used in chemistry or in field evaluations of horticultural plants. There are, however, some generalizations we can make about good experimental designs.

Emphasize comparisons

Experiments include more than just one treatment. “Treatment” refers to the factor that you are varying in your experiment—for example, different cultivars of tomato, different fertilizers, or different amounts of light. Experimental designs incorporate comparison of treatments. You usually compare the treatments to one another and often to a control, which is either the application of no treatment or the application of a customary or standard level of treatment.

If you grow a particular type of tomato in your garden, and find that it produces tasty fruit, would you declare it to be the best tomato variety you could grow? Certainly not. You couldn’t even say with certainty that it was the best tomato variety you have ever grown (unless it is the only one you have grown). Next year, however, you could grow that tomato as your control, and grow two other varieties that your neighbors like, and compare fruit quality (appearance, flavor, yield, sugar content). You could then say something definitive about the three tomato varieties because you have compared them to each other after growing them next to each other in the same year and environment.

Replicate treatments

The same treatment is applied to more than one “experimental unit”—the object that receives the treatment. In the example above, the tomato plant is the experimental unit, and you would perhaps plant two or three seedlings of each tomato variety rather than just one. Think of a treatment as something like a fertilizer spread on a patch of land. The patch of land is the experimental unit, while the fertilizer is the treatment.

By applying the treatment to more than one experimental unit you can estimate the variation you get when two experimental units are treated the same, and compare this to the variation when experimental units are given different treatments. If the treatments actually differ in their effectiveness, you would expect the variation between experimental units given different treatments to be much greater than the variation between those given the same treatment. This is one of the fundamental ways in which experiments are statistically analyzed and treatments declared significantly different or not.

Randomize treatments

Once you know how many treatments you are going to apply, and how many replications you want, the product of these two quantities (# treatments × # replications) equals the number of experimental units you need. For instance, if you have three fertilizers you want to test, plus a control, you have four treatments. If you want three replications of each treatment, then you 4 treatments x 3 replications = 12 experimental units or patches of land where you will apply the fertilizers. The treatments will be randomly assigned to each experimental unit (patch of land). This is done using a random number table and is not just haphazard picking. Randomization helps minimize any bias you haven’t recognized in advance and controlled for in other ways.

• What are two types of control treatments?
• Does increasing the number of replications increase the number of treatments or the number of experimental units?
• Can you think of an example of how randomization can protect against bias?

Process of scientific inquiry; it builds on what is known by testing hypotheses.

A very valuable method for actively learning the body of scientific knowledge that has been previously discovered and communicated by others; and it is specifically orchestrated for teaching and learning, not for the discovery of new information about the world around us.

Typically used during the development of new technologies to identify the best products for the desired purpose (eg. which pesticides are effective against the target insect, but not harmful to non-target insects), but are not used to discover new knowledge about how the world works so they typically don't advance our understanding of the natural world. Used to pick a winner from among a number of options.

Used to verify or regulate a scientific experiment by conducting a parallel experiment or by comparing with another standard

A plant variety that has been produced in cultivation by selective breeding. The term comes from combining the words 'cultivated' and 'variety'.

Process of planning an experiment to test a hypothesis.

An embryonic leaf in seed-bearing plants, one or more of which are the first leaves to appear from a germinating seed.

When the same treatment is applied to more than one experimental unit.

Act of randomly assigning treatments to experimental units using a random number table or computer-generated randomization to help minimize any bias that has not been recognized in advance and controlled for in other ways.

The Science of Plants Copyright © 2022 by The Authors is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License , except where otherwise noted.

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Hypothesis Examples

A hypothesis is a prediction of the outcome of a test. It forms the basis for designing an experiment in the scientific method . A good hypothesis is testable, meaning it makes a prediction you can check with observation or experimentation. Here are different hypothesis examples.

Null Hypothesis Examples

The null hypothesis (H 0 ) is also known as the zero-difference or no-difference hypothesis. It predicts that changing one variable ( independent variable ) will have no effect on the variable being measured ( dependent variable ). Here are null hypothesis examples:

• Plant growth is unaffected by temperature.
• If you increase temperature, then solubility of salt will increase.
• Incidence of skin cancer is unrelated to ultraviolet light exposure.
• All brands of light bulb last equally long.
• Cats have no preference for the color of cat food.
• All daisies have the same number of petals.

Sometimes the null hypothesis shows there is a suspected correlation between two variables. For example, if you think plant growth is affected by temperature, you state the null hypothesis: “Plant growth is not affected by temperature.” Why do you do this, rather than say “If you change temperature, plant growth will be affected”? The answer is because it’s easier applying a statistical test that shows, with a high level of confidence, a null hypothesis is correct or incorrect.

Research Hypothesis Examples

A research hypothesis (H 1 ) is a type of hypothesis used to design an experiment. This type of hypothesis is often written as an if-then statement because it’s easy identifying the independent and dependent variables and seeing how one affects the other. If-then statements explore cause and effect. In other cases, the hypothesis shows a correlation between two variables. Here are some research hypothesis examples:

• If you leave the lights on, then it takes longer for people to fall asleep.
• If you refrigerate apples, they last longer before going bad.
• If you keep the curtains closed, then you need less electricity to heat or cool the house (the electric bill is lower).
• If you leave a bucket of water uncovered, then it evaporates more quickly.
• Goldfish lose their color if they are not exposed to light.
• Workers who take vacations are more productive than those who never take time off.

Is It Okay to Disprove a Hypothesis?

Yes! You may even choose to write your hypothesis in such a way that it can be disproved because it’s easier to prove a statement is wrong than to prove it is right. In other cases, if your prediction is incorrect, that doesn’t mean the science is bad. Revising a hypothesis is common. It demonstrates you learned something you did not know before you conducted the experiment.

Test yourself with a Scientific Method Quiz .

• Mellenbergh, G.J. (2008). Chapter 8: Research designs: Testing of research hypotheses. In H.J. Adèr & G.J. Mellenbergh (eds.), Advising on Research Methods: A Consultant’s Companion . Huizen, The Netherlands: Johannes van Kessel Publishing.
• Popper, Karl R. (1959). The Logic of Scientific Discovery . Hutchinson & Co. ISBN 3-1614-8410-X.
• Schick, Theodore; Vaughn, Lewis (2002). How to think about weird things: critical thinking for a New Age . Boston: McGraw-Hill Higher Education. ISBN 0-7674-2048-9.
• Tobi, Hilde; Kampen, Jarl K. (2018). “Research design: the methodology for interdisciplinary research framework”. Quality & Quantity . 52 (3): 1209–1225. doi: 10.1007/s11135-017-0513-8

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Null Hypothesis Definition and Examples

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In a scientific experiment, the null hypothesis is the proposition that there is no effect or no relationship between phenomena or populations. If the null hypothesis is true, any observed difference in phenomena or populations would be due to sampling error (random chance) or experimental error. The null hypothesis is useful because it can be tested and found to be false, which then implies that there is a relationship between the observed data. It may be easier to think of it as a nullifiable hypothesis or one that the researcher seeks to nullify. The null hypothesis is also known as the H 0, or no-difference hypothesis.

The alternate hypothesis, H A or H 1 , proposes that observations are influenced by a non-random factor. In an experiment, the alternate hypothesis suggests that the experimental or independent variable has an effect on the dependent variable .

How to State a Null Hypothesis

There are two ways to state a null hypothesis. One is to state it as a declarative sentence, and the other is to present it as a mathematical statement.

For example, say a researcher suspects that exercise is correlated to weight loss, assuming diet remains unchanged. The average length of time to achieve a certain amount of weight loss is six weeks when a person works out five times a week. The researcher wants to test whether weight loss takes longer to occur if the number of workouts is reduced to three times a week.

The first step to writing the null hypothesis is to find the (alternate) hypothesis. In a word problem like this, you're looking for what you expect to be the outcome of the experiment. In this case, the hypothesis is "I expect weight loss to take longer than six weeks."

This can be written mathematically as: H 1 : μ > 6

In this example, μ is the average.

Now, the null hypothesis is what you expect if this hypothesis does not happen. In this case, if weight loss isn't achieved in greater than six weeks, then it must occur at a time equal to or less than six weeks. This can be written mathematically as:

H 0 : μ ≤ 6

The other way to state the null hypothesis is to make no assumption about the outcome of the experiment. In this case, the null hypothesis is simply that the treatment or change will have no effect on the outcome of the experiment. For this example, it would be that reducing the number of workouts would not affect the time needed to achieve weight loss:

H 0 : μ = 6

Null Hypothesis Examples

"Hyperactivity is unrelated to eating sugar " is an example of a null hypothesis. If the hypothesis is tested and found to be false, using statistics, then a connection between hyperactivity and sugar ingestion may be indicated. A significance test is the most common statistical test used to establish confidence in a null hypothesis.

Another example of a null hypothesis is "Plant growth rate is unaffected by the presence of cadmium in the soil ." A researcher could test the hypothesis by measuring the growth rate of plants grown in a medium lacking cadmium, compared with the growth rate of plants grown in mediums containing different amounts of cadmium. Disproving the null hypothesis would set the groundwork for further research into the effects of different concentrations of the element in soil.

Why Test a Null Hypothesis?

You may be wondering why you would want to test a hypothesis just to find it false. Why not just test an alternate hypothesis and find it true? The short answer is that it is part of the scientific method. In science, propositions are not explicitly "proven." Rather, science uses math to determine the probability that a statement is true or false. It turns out it's much easier to disprove a hypothesis than to positively prove one. Also, while the null hypothesis may be simply stated, there's a good chance the alternate hypothesis is incorrect.

For example, if your null hypothesis is that plant growth is unaffected by duration of sunlight, you could state the alternate hypothesis in several different ways. Some of these statements might be incorrect. You could say plants are harmed by more than 12 hours of sunlight or that plants need at least three hours of sunlight, etc. There are clear exceptions to those alternate hypotheses, so if you test the wrong plants, you could reach the wrong conclusion. The null hypothesis is a general statement that can be used to develop an alternate hypothesis, which may or may not be correct.

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What Is a Hypothesis and How Do I Write One?

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Think about something strange and unexplainable in your life. Maybe you get a headache right before it rains, or maybe you think your favorite sports team wins when you wear a certain color. If you wanted to see whether these are just coincidences or scientific fact, you would form a hypothesis, then create an experiment to see whether that hypothesis is true or not.

But what is a hypothesis, anyway? If you’re not sure about what a hypothesis is--or how to test for one!--you’re in the right place. This article will teach you everything you need to know about hypotheses, including: 

• Defining the term “hypothesis” 
• Providing hypothesis examples 
• Giving you tips for how to write your own hypothesis

So let’s get started!

What Is a Hypothesis?

Merriam Webster defines a hypothesis as “an assumption or concession made for the sake of argument.” In other words, a hypothesis is an educated guess . Scientists make a reasonable assumption--or a hypothesis--then design an experiment to test whether it’s true or not. Keep in mind that in science, a hypothesis should be testable. You have to be able to design an experiment that tests your hypothesis in order for it to be valid. 

As you could assume from that statement, it’s easy to make a bad hypothesis. But when you’re holding an experiment, it’s even more important that your guesses be good...after all, you’re spending time (and maybe money!) to figure out more about your observation. That’s why we refer to a hypothesis as an educated guess--good hypotheses are based on existing data and research to make them as sound as possible.

Hypotheses are one part of what’s called the scientific method .  Every (good) experiment or study is based in the scientific method. The scientific method gives order and structure to experiments and ensures that interference from scientists or outside influences does not skew the results. It’s important that you understand the concepts of the scientific method before holding your own experiment. Though it may vary among scientists, the scientific method is generally made up of six steps (in order):

• Observation
• Asking questions
• Forming a hypothesis
• Analyze the data
• Communicate your results

You’ll notice that the hypothesis comes pretty early on when conducting an experiment. That’s because experiments work best when they’re trying to answer one specific question. And you can’t conduct an experiment until you know what you’re trying to prove!

Independent and Dependent Variables 

After doing your research, you’re ready for another important step in forming your hypothesis: identifying variables. Variables are basically any factor that could influence the outcome of your experiment . Variables have to be measurable and related to the topic being studied.

There are two types of variables:  independent variables and dependent variables. I ndependent variables remain constant . For example, age is an independent variable; it will stay the same, and researchers can look at different ages to see if it has an effect on the dependent variable. 

Speaking of dependent variables... dependent variables are subject to the influence of the independent variable , meaning that they are not constant. Let’s say you want to test whether a person’s age affects how much sleep they need. In that case, the independent variable is age (like we mentioned above), and the dependent variable is how much sleep a person gets. 

Variables will be crucial in writing your hypothesis. You need to be able to identify which variable is which, as both the independent and dependent variables will be written into your hypothesis. For instance, in a study about exercise, the independent variable might be the speed at which the respondents walk for thirty minutes, and the dependent variable would be their heart rate. In your study and in your hypothesis, you’re trying to understand the relationship between the two variables.

Elements of a Good Hypothesis

The best hypotheses start by asking the right questions . For instance, if you’ve observed that the grass is greener when it rains twice a week, you could ask what kind of grass it is, what elevation it’s at, and if the grass across the street responds to rain in the same way. Any of these questions could become the backbone of experiments to test why the grass gets greener when it rains fairly frequently.

As you’re asking more questions about your first observation, make sure you’re also making more observations . If it doesn’t rain for two weeks and the grass still looks green, that’s an important observation that could influence your hypothesis. You'll continue observing all throughout your experiment, but until the hypothesis is finalized, every observation should be noted.

Finally, you should consult secondary research before writing your hypothesis . Secondary research is comprised of results found and published by other people. You can usually find this information online or at your library. Additionally, m ake sure the research you find is credible and related to your topic. If you’re studying the correlation between rain and grass growth, it would help you to research rain patterns over the past twenty years for your county, published by a local agricultural association. You should also research the types of grass common in your area, the type of grass in your lawn, and whether anyone else has conducted experiments about your hypothesis. Also be sure you’re checking the quality of your research . Research done by a middle school student about what minerals can be found in rainwater would be less useful than an article published by a local university.

Writing Your Hypothesis

Once you’ve considered all of the factors above, you’re ready to start writing your hypothesis. Hypotheses usually take a certain form when they’re written out in a research report.

When you boil down your hypothesis statement, you are writing down your best guess and not the question at hand . This means that your statement should be written as if it is fact already, even though you are simply testing it.

The reason for this is that, after you have completed your study, you'll either accept or reject your if-then or your null hypothesis. All hypothesis testing examples should be measurable and able to be confirmed or denied. You cannot confirm a question, only a statement! 

In fact, you come up with hypothesis examples all the time! For instance, when you guess on the outcome of a basketball game, you don’t say, “Will the Miami Heat beat the Boston Celtics?” but instead, “I think the Miami Heat will beat the Boston Celtics.” You state it as if it is already true, even if it turns out you’re wrong. You do the same thing when writing your hypothesis.

Additionally, keep in mind that hypotheses can range from very specific to very broad.  These hypotheses can be specific, but if your hypothesis testing examples involve a broad range of causes and effects, your hypothesis can also be broad.  

The Two Types of Hypotheses

Now that you understand what goes into a hypothesis, it’s time to look more closely at the two most common types of hypothesis: the if-then hypothesis and the null hypothesis.

#1: If-Then Hypotheses

First of all, if-then hypotheses typically follow this formula:

If ____ happens, then ____ will happen.

The goal of this type of hypothesis is to test the causal relationship between the independent and dependent variable. It’s fairly simple, and each hypothesis can vary in how detailed it can be. We create if-then hypotheses all the time with our daily predictions. Here are some examples of hypotheses that use an if-then structure from daily life: 

• If I get enough sleep, I’ll be able to get more work done tomorrow.
• If the bus is on time, I can make it to my friend’s birthday party. 
• If I study every night this week, I’ll get a better grade on my exam. 

In each of these situations, you’re making a guess on how an independent variable (sleep, time, or studying) will affect a dependent variable (the amount of work you can do, making it to a party on time, or getting better grades). 

You may still be asking, “What is an example of a hypothesis used in scientific research?” Take one of the hypothesis examples from a real-world study on whether using technology before bed affects children’s sleep patterns. The hypothesis read s:

“We hypothesized that increased hours of tablet- and phone-based screen time at bedtime would be inversely correlated with sleep quality and child attention.”

It might not look like it, but this is an if-then statement. The researchers basically said, “If children have more screen usage at bedtime, then their quality of sleep and attention will be worse.” The sleep quality and attention are the dependent variables and the screen usage is the independent variable. (Usually, the independent variable comes after the “if” and the dependent variable comes after the “then,” as it is the independent variable that affects the dependent variable.) This is an excellent example of how flexible hypothesis statements can be, as long as the general idea of “if-then” and the independent and dependent variables are present.

#2: Null Hypotheses

Your if-then hypothesis is not the only one needed to complete a successful experiment, however. You also need a null hypothesis to test it against. In its most basic form, the null hypothesis is the opposite of your if-then hypothesis . When you write your null hypothesis, you are writing a hypothesis that suggests that your guess is not true, and that the independent and dependent variables have no relationship .

One null hypothesis for the cell phone and sleep study from the last section might say: 

“If children have more screen usage at bedtime, their quality of sleep and attention will not be worse.” 

In this case, this is a null hypothesis because it’s asking the opposite of the original thesis! 

Conversely, if your if-then hypothesis suggests that your two variables have no relationship, then your null hypothesis would suggest that there is one. So, pretend that there is a study that is asking the question, “Does the amount of followers on Instagram influence how long people spend on the app?” The independent variable is the amount of followers, and the dependent variable is the time spent. But if you, as the researcher, don’t think there is a relationship between the number of followers and time spent, you might write an if-then hypothesis that reads:

“If people have many followers on Instagram, they will not spend more time on the app than people who have less.”

In this case, the if-then suggests there isn’t a relationship between the variables. In that case, one of the null hypothesis examples might say:

“If people have many followers on Instagram, they will spend more time on the app than people who have less.”

You then test both the if-then and the null hypothesis to gauge if there is a relationship between the variables, and if so, how much of a relationship. 

4 Tips to Write the Best Hypothesis

If you’re going to take the time to hold an experiment, whether in school or by yourself, you’re also going to want to take the time to make sure your hypothesis is a good one. The best hypotheses have four major elements in common: plausibility, defined concepts, observability, and general explanation.

#1: Plausibility

At first glance, this quality of a hypothesis might seem obvious. When your hypothesis is plausible, that means it’s possible given what we know about science and general common sense. However, improbable hypotheses are more common than you might think. 

Imagine you’re studying weight gain and television watching habits. If you hypothesize that people who watch more than  twenty hours of television a week will gain two hundred pounds or more over the course of a year, this might be improbable (though it’s potentially possible). Consequently, c ommon sense can tell us the results of the study before the study even begins.

Improbable hypotheses generally go against  science, as well. Take this hypothesis example: 

“If a person smokes one cigarette a day, then they will have lungs just as healthy as the average person’s.” 

This hypothesis is obviously untrue, as studies have shown again and again that cigarettes negatively affect lung health. You must be careful that your hypotheses do not reflect your own personal opinion more than they do scientifically-supported findings. This plausibility points to the necessity of research before the hypothesis is written to make sure that your hypothesis has not already been disproven.

#2: Defined Concepts

The more advanced you are in your studies, the more likely that the terms you’re using in your hypothesis are specific to a limited set of knowledge. One of the hypothesis testing examples might include the readability of printed text in newspapers, where you might use words like “kerning” and “x-height.” Unless your readers have a background in graphic design, it’s likely that they won’t know what you mean by these terms. Thus, it’s important to either write what they mean in the hypothesis itself or in the report before the hypothesis.

Here’s what we mean. Which of the following sentences makes more sense to the common person?

If the kerning is greater than average, more words will be read per minute.

If the space between letters is greater than average, more words will be read per minute.

For people reading your report that are not experts in typography, simply adding a few more words will be helpful in clarifying exactly what the experiment is all about. It’s always a good idea to make your research and findings as accessible as possible. 

Good hypotheses ensure that you can observe the results. 

#3: Observability

In order to measure the truth or falsity of your hypothesis, you must be able to see your variables and the way they interact. For instance, if your hypothesis is that the flight patterns of satellites affect the strength of certain television signals, yet you don’t have a telescope to view the satellites or a television to monitor the signal strength, you cannot properly observe your hypothesis and thus cannot continue your study.

Some variables may seem easy to observe, but if you do not have a system of measurement in place, you cannot observe your hypothesis properly. Here’s an example: if you’re experimenting on the effect of healthy food on overall happiness, but you don’t have a way to monitor and measure what “overall happiness” means, your results will not reflect the truth. Monitoring how often someone smiles for a whole day is not reasonably observable, but having the participants state how happy they feel on a scale of one to ten is more observable. 

In writing your hypothesis, always keep in mind how you'll execute the experiment.

#4: Generalizability 

Perhaps you’d like to study what color your best friend wears the most often by observing and documenting the colors she wears each day of the week. This might be fun information for her and you to know, but beyond you two, there aren’t many people who could benefit from this experiment. When you start an experiment, you should note how generalizable your findings may be if they are confirmed. Generalizability is basically how common a particular phenomenon is to other people’s everyday life.

Let’s say you’re asking a question about the health benefits of eating an apple for one day only, you need to realize that the experiment may be too specific to be helpful. It does not help to explain a phenomenon that many people experience. If you find yourself with too specific of a hypothesis, go back to asking the big question: what is it that you want to know, and what do you think will happen between your two variables?

Hypothesis Testing Examples

We know it can be hard to write a good hypothesis unless you’ve seen some good hypothesis examples. We’ve included four hypothesis examples based on some made-up experiments. Use these as templates or launch pads for coming up with your own hypotheses.

Experiment #1: Students Studying Outside (Writing a Hypothesis)

You are a student at PrepScholar University. When you walk around campus, you notice that, when the temperature is above 60 degrees, more students study in the quad. You want to know when your fellow students are more likely to study outside. With this information, how do you make the best hypothesis possible?

You must remember to make additional observations and do secondary research before writing your hypothesis. In doing so, you notice that no one studies outside when it’s 75 degrees and raining, so this should be included in your experiment. Also, studies done on the topic beforehand suggested that students are more likely to study in temperatures less than 85 degrees. With this in mind, you feel confident that you can identify your variables and write your hypotheses:

If-then: “If the temperature in Fahrenheit is less than 60 degrees, significantly fewer students will study outside.”

Null: “If the temperature in Fahrenheit is less than 60 degrees, the same number of students will study outside as when it is more than 60 degrees.”

These hypotheses are plausible, as the temperatures are reasonably within the bounds of what is possible. The number of people in the quad is also easily observable. It is also not a phenomenon specific to only one person or at one time, but instead can explain a phenomenon for a broader group of people.

To complete this experiment, you pick the month of October to observe the quad. Every day (except on the days where it’s raining)from 3 to 4 PM, when most classes have released for the day, you observe how many people are on the quad. You measure how many people come  and how many leave. You also write down the temperature on the hour. 

After writing down all of your observations and putting them on a graph, you find that the most students study on the quad when it is 70 degrees outside, and that the number of students drops a lot once the temperature reaches 60 degrees or below. In this case, your research report would state that you accept or “failed to reject” your first hypothesis with your findings.

Experiment #2: The Cupcake Store (Forming a Simple Experiment)

Let’s say that you work at a bakery. You specialize in cupcakes, and you make only two colors of frosting: yellow and purple. You want to know what kind of customers are more likely to buy what kind of cupcake, so you set up an experiment. Your independent variable is the customer’s gender, and the dependent variable is the color of the frosting. What is an example of a hypothesis that might answer the question of this study?

Here’s what your hypotheses might look like: 

If-then: “If customers’ gender is female, then they will buy more yellow cupcakes than purple cupcakes.”

Null: “If customers’ gender is female, then they will be just as likely to buy purple cupcakes as yellow cupcakes.”

This is a pretty simple experiment! It passes the test of plausibility (there could easily be a difference), defined concepts (there’s nothing complicated about cupcakes!), observability (both color and gender can be easily observed), and general explanation ( this would potentially help you make better business decisions ).

Experiment #3: Backyard Bird Feeders (Integrating Multiple Variables and Rejecting the If-Then Hypothesis)

While watching your backyard bird feeder, you realized that different birds come on the days when you change the types of seeds. You decide that you want to see more cardinals in your backyard, so you decide to see what type of food they like the best and set up an experiment. 

However, one morning, you notice that, while some cardinals are present, blue jays are eating out of your backyard feeder filled with millet. You decide that, of all of the other birds, you would like to see the blue jays the least. This means you'll have more than one variable in your hypothesis. Your new hypotheses might look like this: 

If-then: “If sunflower seeds are placed in the bird feeders, then more cardinals will come than blue jays. If millet is placed in the bird feeders, then more blue jays will come than cardinals.”

Null: “If either sunflower seeds or millet are placed in the bird, equal numbers of cardinals and blue jays will come.”

Through simple observation, you actually find that cardinals come as often as blue jays when sunflower seeds or millet is in the bird feeder. In this case, you would reject your “if-then” hypothesis and “fail to reject” your null hypothesis . You cannot accept your first hypothesis, because it’s clearly not true. Instead you found that there was actually no relation between your different variables. Consequently, you would need to run more experiments with different variables to see if the new variables impact the results.

Experiment #4: In-Class Survey (Including an Alternative Hypothesis)

You’re about to give a speech in one of your classes about the importance of paying attention. You want to take this opportunity to test a hypothesis you’ve had for a while: 

If-then: If students sit in the first two rows of the classroom, then they will listen better than students who do not.

Null: If students sit in the first two rows of the classroom, then they will not listen better or worse than students who do not.

You give your speech and then ask your teacher if you can hand out a short survey to the class. On the survey, you’ve included questions about some of the topics you talked about. When you get back the results, you’re surprised to see that not only do the students in the first two rows not pay better attention, but they also scored worse than students in other parts of the classroom! Here, both your if-then and your null hypotheses are not representative of your findings. What do you do?

This is when you reject both your if-then and null hypotheses and instead create an alternative hypothesis . This type of hypothesis is used in the rare circumstance that neither of your hypotheses is able to capture your findings . Now you can use what you’ve learned to draft new hypotheses and test again! 

Key Takeaways: Hypothesis Writing

The more comfortable you become with writing hypotheses, the better they will become. The structure of hypotheses is flexible and may need to be changed depending on what topic you are studying. The most important thing to remember is the purpose of your hypothesis and the difference between the if-then and the null . From there, in forming your hypothesis, you should constantly be asking questions, making observations, doing secondary research, and considering your variables. After you have written your hypothesis, be sure to edit it so that it is plausible, clearly defined, observable, and helpful in explaining a general phenomenon.

Writing a hypothesis is something that everyone, from elementary school children competing in a science fair to professional scientists in a lab, needs to know how to do. Hypotheses are vital in experiments and in properly executing the scientific method . When done correctly, hypotheses will set up your studies for success and help you to understand the world a little better, one experiment at a time.

What’s Next?

If you’re studying for the science portion of the ACT, there’s definitely a lot you need to know. We’ve got the tools to help, though! Start by checking out our ultimate study guide for the ACT Science subject test. Once you read through that, be sure to download our recommended ACT Science practice tests , since they’re one of the most foolproof ways to improve your score. (And don’t forget to check out our expert guide book , too.)

If you love science and want to major in a scientific field, you should start preparing in high school . Here are the science classes you should take to set yourself up for success.

If you’re trying to think of science experiments you can do for class (or for a science fair!), here’s a list of 37 awesome science experiments you can do at home

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Ashley Sufflé Robinson has a Ph.D. in 19th Century English Literature. As a content writer for PrepScholar, Ashley is passionate about giving college-bound students the in-depth information they need to get into the school of their dreams.

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On the scope of scientific hypotheses

William hedley thompson.

1 Department of Applied Information Technology, University of Gothenburg, Gothenburg, Sweden

2 Institute of Neuroscience and Physiology, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden

3 Department of Pedagogical, Curricular and Professional Studies, Faculty of Education, University of Gothenburg, Gothenburg, Sweden

4 Department of Clinical Neuroscience, Karolinska Institutet, Stockholm, Sweden

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Hypotheses are frequently the starting point when undertaking the empirical portion of the scientific process. They state something that the scientific process will attempt to evaluate, corroborate, verify or falsify. Their purpose is to guide the types of data we collect, analyses we conduct, and inferences we would like to make. Over the last decade, metascience has advocated for hypotheses being in preregistrations or registered reports, but how to formulate these hypotheses has received less attention. Here, we argue that hypotheses can vary in specificity along at least three independent dimensions: the relationship, the variables, and the pipeline. Together, these dimensions form the scope of the hypothesis. We demonstrate how narrowing the scope of a hypothesis in any of these three ways reduces the hypothesis space and that this reduction is a type of novelty. Finally, we discuss how this formulation of hypotheses can guide researchers to formulate the appropriate scope for their hypotheses and should aim for neither too broad nor too narrow a scope. This framework can guide hypothesis-makers when formulating their hypotheses by helping clarify what is being tested, chaining results to previous known findings, and demarcating what is explicitly tested in the hypothesis.

1.  Introduction

Hypotheses are an important part of the scientific process. However, surprisingly little attention is given to hypothesis-making compared to other skills in the scientist's skillset within current discussions aimed at improving scientific practice. Perhaps this lack of emphasis is because the formulation of the hypothesis is often considered less relevant, as it is ultimately the scientific process that will eventually decide the veracity of the hypothesis. However, there are more hypotheses than scientific studies as selection occurs at various stages: from funder selection and researcher's interests. So which hypotheses are worthwhile to pursue? Which hypotheses are the most effective or pragmatic for extending or enhancing our collective knowledge? We consider the answer to these questions by discussing how broad or narrow a hypothesis can or should be (i.e. its scope).

We begin by considering that the two statements below are both hypotheses and vary in scope:

• H 1 : For every 1 mg decrease of x , y will increase by, on average, 2.5 points.
• H 2 : Changes in x 1 or x 2 correlate with y levels in some way.

Clearly, the specificity of the two hypotheses is very different. H 1 states a precise relationship between two variables ( x and y ), while H 2 specifies a vaguer relationship and does not specify which variables will show the relationship. However, they are both still hypotheses about how x and y relate to each other. This claim of various degrees of the broadness of hypotheses is, in and of itself, not novel. In Epistemetrics, Rescher [ 1 ], while drawing upon the physicist Duhem's work, develops what he calls Duhem's Law. This law considers a trade-off between certainty or precision in statements about physics when evaluating them. Duhem's Law states that narrower hypotheses, such as H 1 above, are more precise but less likely to be evaluated as true than broader ones, such as H 2 above. Similarly, Popper, when discussing theories, describes the reverse relationship between content and probability of a theory being true, i.e. with increased content, there is a decrease in probability and vice versa [ 2 ]. Here we will argue that it is important that both H 1 and H 2 are still valid scientific hypotheses, and their appropriateness depends on certain scientific questions.

The question of hypothesis scope is relevant since there are multiple recent prescriptions to improve science, ranging from topics about preregistrations [ 3 ], registered reports [ 4 ], open science [ 5 ], standardization [ 6 ], generalizability [ 7 ], multiverse analyses [ 8 ], dataset reuse [ 9 ] and general questionable research practices [ 10 ]. Within each of these issues, there are arguments to demarcate between confirmatory and exploratory research or normative prescriptions about how science should be done (e.g. science is ‘bad’ or ‘worse’ if code/data are not open). Despite all these discussions and improvements, much can still be done to improve hypothesis-making. A recent evaluation of preregistered studies in psychology found that over half excluded the preregistered hypotheses [ 11 ]. Further, evaluations of hypotheses in ecology showed that most hypotheses are not explicitly stated [ 12 , 13 ]. Other research has shown that obfuscated hypotheses are more prevalent in retracted research [ 14 ]. There have been recommendations for simpler hypotheses in psychology to avoid misinterpretations and misspecifications [ 15 ]. Finally, several evaluations of preregistration practices have found that a significant proportion of articles do not abide by their stated hypothesis or add additional hypotheses [ 11 , 16 – 18 ]. In sum, while multiple efforts exist to improve scientific practice, our hypothesis-making could improve.

One of our intentions is to provide hypothesis-makers with tools to assist them when making hypotheses. We consider this useful and timely as, with preregistrations becoming more frequent, the hypothesis-making process is now open and explicit . However, preregistrations are difficult to write [ 19 ], and preregistered articles can change or omit hypotheses [ 11 ] or they are vague and certain degrees of freedom hard to control for [ 16 – 18 ]. One suggestion has been to do less confirmatory research [ 7 , 20 ]. While we agree that all research does not need to be confirmatory, we also believe that not all preregistrations of confirmatory work must test narrow hypotheses. We think there is a possible point of confusion that the specificity in preregistrations, where researcher degrees of freedom should be stated, necessitates the requirement that the hypothesis be narrow. Our belief that this confusion is occurring is supported by the study Akker et al . [ 11 ] where they found that 18% of published psychology studies changed their preregistered hypothesis (e.g. its direction), and 60% of studies selectively reported hypotheses in some way. It is along these lines that we feel the framework below can be useful to help formulate appropriate hypotheses to mitigate these identified issues.

We consider this article to be a discussion of the researcher's different choices when formulating hypotheses and to help link hypotheses over time. Here we aim to deconstruct what aspects there are in the hypothesis about their specificity. Throughout this article, we intend to be neutral to many different philosophies of science relating to the scientific method (i.e. how one determines the veracity of a hypothesis). Our idea of neutrality here is that whether a researcher adheres to falsification, verification, pragmatism, or some other philosophy of science, then this framework can be used when formulating hypotheses. 1

The framework this article advocates for is that there are (at least) three dimensions that hypotheses vary along regarding their narrowness and broadness: the selection of relationships, variables, and pipelines. We believe this discussion is fruitful for the current debate regarding normative practices as some positions make, sometimes implicit, commitments about which set of hypotheses the scientific community ought to consider good or permissible. We proceed by outlining a working definition of ‘scientific hypothesis' and then discuss how it relates to theory. Then, we justify how hypotheses can vary along the three dimensions. Using this framework, we then discuss the scopes in relation to appropriate hypothesis-making and an argument about what constitutes a scientifically novel hypothesis. We end the article with practical advice for researchers who wish to use this framework.

2.  The scientific hypothesis

In this section, we will describe a functional and descriptive role regarding how scientists use hypotheses. Jeong & Kwon [ 21 ] investigated and summarized the different uses the concept of ‘hypothesis’ had in philosophical and scientific texts. They identified five meanings: assumption, tentative explanation, tentative cause, tentative law, and prediction. Jeong & Kwon [ 21 ] further found that researchers in science and philosophy used all the different definitions of hypotheses, although there was some variance in frequency between fields. Here we see, descriptively , that the way researchers use the word ‘hypothesis’ is diverse and has a wide range in specificity and function. However, whichever meaning a hypothesis has, it aims to be true, adequate, accurate or useful in some way.

Not all hypotheses are ‘scientific hypotheses'. For example, consider the detective trying to solve a crime and hypothesizing about the perpetrator. Such a hypothesis still aims to be true and is a tentative explanation but differs from the scientific hypothesis. The difference is that the researcher, unlike the detective, evaluates the hypothesis with the scientific method and submits the work for evaluation by the scientific community. Thus a scientific hypothesis entails a commitment to evaluate the statement with the scientific process . 2 Additionally, other types of hypotheses can exist. As discussed in more detail below, scientific theories generate not only scientific hypotheses but also contain auxiliary hypotheses. The latter refers to additional assumptions considered to be true and not explicitly evaluated. 3

Next, the scientific hypothesis is generally made antecedent to the evaluation. This does not necessitate that the event (e.g. in archaeology) or the data collection (e.g. with open data reuse) must be collected before the hypothesis is made, but that the evaluation of the hypothesis cannot happen before its formulation. This claim state does deny the utility of exploratory hypothesis testing of post hoc hypotheses (see [ 25 ]). However, previous results and exploration can generate new hypotheses (e.g. via abduction [ 22 , 26 – 28 ], which is the process of creating hypotheses from evidence), which is an important part of science [ 29 – 32 ], but crucially, while these hypotheses are important and can be the conclusion of exploratory work, they have yet to be evaluated (by whichever method of choice). Hence, they still conform to the antecedency requirement. A further way to justify the antecedency is seen in the practice of formulating a post hoc hypothesis, and considering it to have been evaluated is seen as a questionable research practice (known as ‘hypotheses after results are known’ or HARKing [ 33 ]). 4

While there is a varying range of specificity, is the hypothesis a critical part of all scientific work, or is it reserved for some subset of investigations? There are different opinions regarding this. Glass and Hall, for example, argue that the term only refers to falsifiable research, and model-based research uses verification [ 36 ]. However, this opinion does not appear to be the consensus. Osimo and Rumiati argue that any model based on or using data is never wholly free from hypotheses, as hypotheses can, even implicitly, infiltrate the data collection [ 37 ]. For our definition, we will consider hypotheses that can be involved in different forms of scientific evaluation (i.e. not just falsification), but we do not exclude the possibility of hypothesis-free scientific work.

Finally, there is a debate about whether theories or hypotheses should be linguistic or formal [ 38 – 40 ]. Neither side in this debate argues that verbal or formal hypotheses are not possible, but instead, they discuss normative practices. Thus, for our definition, both linguistic and formal hypotheses are considered viable.

Considering the above discussion, let us summarize the scientific process and the scientific hypothesis: a hypothesis guides what type of data are sampled and what analysis will be done. With the new observations, evidence is analysed or quantified in some way (often using inferential statistics) to judge the hypothesis's truth value, utility, credibility, or likelihood. The following working definition captures the above:

• Scientific hypothesis : an implicit or explicit statement that can be verbal or formal. The hypothesis makes a statement about some natural phenomena (via an assumption, explanation, cause, law or prediction). The scientific hypothesis is made antecedent to performing a scientific process where there is a commitment to evaluate it.

For simplicity, we will only use the term ‘hypothesis’ for ‘scientific hypothesis' to refer to the above definition for the rest of the article except when it is necessary to distinguish between other types of hypotheses. Finally, this definition could further be restrained in multiple ways (e.g. only explicit hypotheses are allowed, or assumptions are never hypotheses). However, if the definition is more (or less) restrictive, it has little implication for the argument below.

3.  The hypothesis, theory and auxiliary assumptions

While we have a definition of the scientific hypothesis, we have yet to link it with how it relates to scientific theory, where there is frequently some interconnection (i.e. a hypothesis tests a scientific theory). Generally, for this paper, we believe our argument applies regardless of how scientific theory is defined. Further, some research lacks theory, sometimes called convenience or atheoretical studies [ 41 ]. Here a hypothesis can be made without a wider theory—and our framework fits here too. However, since many consider hypotheses to be defined or deducible from scientific theory, there is an important connection between the two. Therefore, we will briefly clarify how hypotheses relate to common formulations of scientific theory.

A scientific theory is generally a set of axioms or statements about some objects, properties and their relations relating to some phenomena. Hypotheses can often be deduced from the theory. Additionally, a theory has boundary conditions. The boundary conditions specify the domain of the theory stating under what conditions it applies (e.g. all things with a central neural system, humans, women, university teachers) [ 42 ]. Boundary conditions of a theory will consequently limit all hypotheses deduced from the theory. For example, with a boundary condition ‘applies to all humans’, then the subsequent hypotheses deduced from the theory are limited to being about humans. While this limitation of the hypothesis by the theory's boundary condition exists, all the considerations about a hypothesis scope detailed below still apply within the boundary conditions. Finally, it is also possible (depending on the definition of scientific theory) for a hypothesis to test the same theory under different boundary conditions. 5

The final consideration relating scientific theory to scientific hypotheses is auxiliary hypotheses. These hypotheses are theories or assumptions that are considered true simultaneously with the theory. Most philosophies of science from Popper's background knowledge [ 24 ], Kuhn's paradigms during normal science [ 44 ], and Laktos' protective belt [ 45 ] all have their own versions of this auxiliary or background information that is required for the hypothesis to test the theory. For example, Meelh [ 46 ] auxiliary theories/assumptions are needed to go from theoretical terms to empirical terms (e.g. neural activity can be inferred from blood oxygenation in fMRI research or reaction time to an indicator of cognition) and auxiliary theories about instruments (e.g. the experimental apparatus works as intended) and more (see also Other approaches to categorizing hypotheses below). As noted in the previous section, there is a difference between these auxiliary hypotheses, regardless of their definition, and the scientific hypothesis defined above. Recall that our definition of the scientific hypothesis included a commitment to evaluate it. There are no such commitments with auxiliary hypotheses, but rather they are assumed to be correct to test the theory adequately. This distinction proves to be important as auxiliary hypotheses are still part of testing a theory but are separate from the hypothesis to be evaluated (discussed in more detail below).

4.  The scope of hypotheses

In the scientific hypothesis section, we defined the hypothesis and discussed how it relates back to the theory. In this section, we want to defend two claims about hypotheses:

• (A1) Hypotheses can have different scopes . Some hypotheses are narrower in their formulation, and some are broader.
• (A2) The scope of hypotheses can vary along three dimensions relating to relationship selection , variable selection , and pipeline selection .

A1 may seem obvious, but it is important to establish what is meant by narrower and broader scope. When a hypothesis is very narrow, it is specific. For example, it might be specific about the type of relationship between some variables. In figure 1 , we make four different statements regarding the relationship between x and y . The narrowest hypothesis here states ‘there is a positive linear relationship with a magnitude of 0.5 between x and y ’ ( figure 1 a ), and the broadest hypothesis states ‘there is a relationship between x and y ’ ( figure 1 d ). Note that many other hypotheses are possible that are not included in this example (such as there being no relationship).

Examples of narrow and broad hypotheses between x and y . Circles indicate a set of possible relationships with varying slopes that can pivot or bend.

We see that the narrowest of these hypotheses claims a type of relationship (linear), a direction of the relationship (positive) and a magnitude of the relationship (0.5). As the hypothesis becomes broader, the specific magnitude disappears ( figure 1 b ), the relationship has additional options than just being linear ( figure 1 c ), and finally, the direction of the relationship disappears. Crucially, all the examples in figure 1 can meet the above definition of scientific hypotheses. They are all statements that can be evaluated with the same scientific method. There is a difference between these statements, though— they differ in the scope of the hypothesis . Here we have justified A1.

Within this framework, when we discuss whether a hypothesis is narrower or broader in scope, this is a relation between two hypotheses where one is a subset of the other. This means that if H 1 is narrower than H 2 , and if H 1 is true, then H 2 is also true. This can be seen in figure 1 a–d . Suppose figure 1 a , the narrowest of all the hypotheses, is true. In that case, all the other broader statements are also true (i.e. a linear correlation of 0.5 necessarily entails that there is also a positive linear correlation, a linear correlation, and some relationship). While this property may appear trivial, it entails that it is only possible to directly compare the hypothesis scope between two hypotheses (i.e. their broadness or narrowness) where one is the subset of the other. 6

4.1. Sets, disjunctions and conjunctions of elements

The above restraint defines the scope as relations between sets. This property helps formalize the framework of this article. Below, when we discuss the different dimensions that can impact the scope, these become represented as a set. Each set contains elements. Each element is a permissible situation that allows the hypothesis to be accepted. We denote elements as lower case with italics (e.g. e 1 , e 2 , e 3 ) and sets as bold upper case (e.g. S ). Each of the three different dimensions discussed below will be formalized as sets, while the total number of elements specifies their scope.

Let us reconsider the above restraint about comparing hypotheses as narrower or broader. This can be formally shown if:

• e 1 , e 2 , e 3 are elements of S 1 ; and
• e 1 and e 2 are elements of S 2 ,

then S 2 is narrower than S 1 .

Each element represents specific propositions that, if corroborated, would support the hypothesis. Returning to figure 1 a , b , the following statements apply to both:

• ‘There is a positive linear relationship between x and y with a slope of 0.5’.

Whereas the following two apply to figure 1 b but not figure 1 a :

• ‘There is a positive linear relationship between x and y with a slope of 0.4’ ( figure 1 b ).
• ‘There is a positive linear relationship between x and y with a slope of 0.3’ ( figure 1 b ).

Figure 1 b allows for a considerably larger number of permissible situations (which is obvious as it allows for any positive linear relationship). When formulating the hypothesis in figure 1 b , we do not need to specify every single one of these permissible relationships. We can simply specify all possible positive slopes, which entails the set of permissible elements it includes.

That broader hypotheses have more elements in their sets entails some important properties. When we say S contains the elements e 1 , e 2 , and e 3 , the hypothesis is corroborated if e 1 or e 2 or e 3 is the case. This means that the set requires only one of the elements to be corroborated for the hypothesis to be considered correct (i.e. the positive linear relationship needs to be 0.3 or 0.4 or 0.5). Contrastingly, we will later see cases when conjunctions of elements occur (i.e. both e 1 and e 2 are the case). When a conjunction occurs, in this formulation, the conjunction itself becomes an element in the set (i.e. ‘ e 1 and e 2 ’ is a single element). Figure 2 illustrates how ‘ e 1 and e 2 ’ is narrower than ‘ e 1 ’, and ‘ e 1 ’ is narrower than ‘ e 1 or e 2 ’. 7 This property relating to the conjunction being narrower than individual elements is explained in more detail in the pipeline selection section below.

Scope as sets. Left : four different sets (grey, red, blue and purple) showing different elements which they contain. Right : a list of each colour explaining which set is a subset of the other (thereby being ‘narrower’).

4.2. Relationship selection

We move to A2, which is to show the different dimensions that a hypothesis scope can vary along. We have already seen an example of the first dimension of a hypothesis in figure 1 , the relationship selection . Let R denote the set of all possible configurations of relationships that are permissible for the hypothesis to be considered true. For example, in the narrowest formulation above, there was one allowed relationship for the hypothesis to be true. Consequently, the size of R (denoted | R |) is one. As discussed above, in the second narrowest formulation ( figure 1 b ), R has more possible relationships where it can still be considered true:

• r 1 = ‘a positive linear relationship of 0.1’
• r 2 = ‘a positive linear relationship of 0.2’
• r 3 = ‘a positive linear relationship of 0.3’.

Additionally, even broader hypotheses will be compatible with more types of relationships. In figure 1 c , d , nonlinear and negative relationships are also possible relationships included in R . For this broader statement to be affirmed, more elements are possible to be true. Thus if | R | is greater (i.e. contains more possible configurations for the hypothesis to be true), then the hypothesis is broader. Thus, the scope of relating to the relationship selection is specified by | R |. Finally, if |R H1 | > |R H2 | , then H 1 is broader than H 2 regarding the relationship selection.

Figure 1 is an example of the relationship narrowing. That the relationship became linear is only an example and does not necessitate a linear relationship or that this scope refers only to correlations. An alternative example of a relationship scope is a broad hypothesis where there is no knowledge about the distribution of some data. In such situations, one may assume a uniform relationship or a Cauchy distribution centred at zero. Over time the specific distribution can be hypothesized. Thereafter, the various parameters of the distribution can be hypothesized. At each step, the hypothesis of the distribution gets further specified to narrower formulations where a smaller set of possible relationships are included (see [ 47 , 48 ] for a more in-depth discussion about how specific priors relate to more narrow tests). Finally, while figure 1 was used to illustrate the point of increasingly narrow relationship hypotheses, it is more likely to expect the narrowest relationship, within fields such as psychology, to have considerable uncertainty and be formulated with confidence or credible intervals (i.e. we will rarely reach point estimates).

4.3. Variable selection

We have demonstrated that relationship selection can affect the scope of a hypothesis. Additionally, at least two other dimensions can affect the scope of a hypothesis: variable selection and pipeline selection . The variable selection in figure 1 was a single bivariate relationship (e.g. x 's relationship with y ). However, it is not always the case that we know which variables will be involved. For example, in neuroimaging, we can be confident that one or more brain regions will be processing some information following a stimulus. Still, we might not be sure which brain region(s) this will be. Consequently, our hypothesis becomes broader because we have selected more variables. The relationship selection may be identical for each chosen variable, but the variable selection becomes broader. We can consider the following three hypotheses to be increasing in their scope:

• H 1 : x relates to y with relationship R .
• H 2 : x 1 or x 2 relates to y with relationship R .
• H 3 : x 1 or x 2 or x 3 relates to y with relationship R .

For H 1 –H 3 above, we assume that R is the same. Further, we assume that there is no interaction between these variables.

In the above examples, we have multiple x ( x 1 , x 2 , x 3 , … , x n ). Again, we can symbolize the variable selection as a non-empty set XY , containing either a single variable or many variables. Our motivation for designating it XY is that the variable selection can include multiple possibilities for both the independent variable ( x ) and the dependent variable ( y ). Like with relationship selection, we can quantify the broadness between two hypotheses with the size of the set XY . Consequently, | XY | denotes the total scope concerning variable selection. Thus, in the examples above | XY H1 | < | XY H2 | < | XY H3 |. Like with relationship selection, hypotheses that vary in | XY | still meet the definition of a hypothesis. 8

An obvious concern for many is that a broader XY is much easier to evaluate as correct. Generally, when | XY 1 | > | XY 2 |, there is a greater chance of spurious correlations when evaluating XY 1 . This concern is an issue relating to the evaluation of hypotheses (e.g. applying statistics to the evaluation), which will require additional assumptions relating to how to evaluate the hypotheses. Strategies to deal with this apply some correction or penalization for multiple statistical testing [ 49 ] or partial pooling and regularizing priors [ 50 , 51 ]. These strategies aim to evaluate a broader variable selection ( x 1 or x 2 ) on equal or similar terms to a narrow variable selection ( x 1 ).

4.4. Pipeline selection

Scientific studies require decisions about how to perform the analysis. This scope considers transformations applied to the raw data ( XY raw ) to achieve some derivative ( XY ). These decisions can also involve selection procedures that drop observations deemed unreliable, standardizing, correcting confounding variables, or different philosophies. We can call the array of decisions and transformations used as the pipeline . A hypothesis varies in the number of pipelines:

• H 1 : XY has a relationship(s) R with pipeline p 1 .
• H 2 : XY has a relationship(s) R with pipeline p 1 or pipeline p 2 .
• H 3 : XY has a relationship(s) R with pipeline p 1 or pipeline p 2 , or pipeline p 3 .

Importantly, the pipeline here considers decisions regarding how the hypothesis shapes the data collection and transformation. We do not consider this to include decisions made regarding the assumptions relating to the statistical inference as those relate to operationalizing the evaluation of the hypothesis and not part of the hypothesis being evaluated (these assumptions are like auxiliary hypotheses, which are assumed to be true but not explicitly evaluated).

Like with variable selection ( XY ) and relationship selection ( R ), we can see that pipelines impact the scope of hypotheses. Again, we can symbolize the pipeline selection with a set P . As previously, | P | will denote the dimension of the pipeline selection. In the case of pipeline selection, we are testing the same variables, looking for the same relationship, but processing the variables or relationships with different pipelines to evaluate the relationship. Consequently, | P H1 | < | P H2 | < | P H3 |.

These issues regarding pipelines have received attention as the ‘garden of forking paths' [ 52 ]. Here, there are calls for researchers to ensure that their entire pipeline has been specified. Additionally, recent work has highlighted the diversity of results based on multiple analytical pipelines [ 53 , 54 ]. These results are often considered a concern, leading to calls that results should be pipeline resistant.

The wish for pipeline-resistant methods entails that hypotheses, in their narrowest form, are possible for all pipelines. Consequently, a narrower formulation will entail that this should not impact the hypothesis regardless of which pipeline is chosen. Thus the conjunction of pipelines is narrower than single pipelines. Consider the following three scenarios:

• H 3 : XY has a relationship(s) R with pipeline p 1 and pipeline p 2 .

In this instance, since H 1 is always true if H 3 is true, thus H 3 is a narrower formulation than H 1 . Consequently, | P H3 | < | P H1 | < | P H2 |. Decreasing the scope of the pipeline dimension also entails the increase in conjunction of pipelines (i.e. creating pipeline-resistant methods) rather than just the reduction of disjunctional statements.

4.5. Combining the dimensions

In summary, we then have three different dimensions that independently affect the scope of the hypothesis. We have demonstrated the following general claim regarding hypotheses:

• The variables XY have a relationship R with pipeline P .

And that the broadness and narrowness of a hypothesis depend on how large the three sets XY , R and P are. With this formulation, we can conclude that hypotheses have a scope that can be determined with a 3-tuple argument of (| R |, | XY |, | P |).

While hypotheses can be formulated along these three dimensions and generally aim to be reduced, it does not entail that these dimensions behave identically. For example, the relationship dimensions aim to reduce the number of elements as far as possible (e.g. to an interval). Contrastingly, for both variables and pipeline, the narrower hypothesis can reduce to single variables/pipelines or become narrower still and become conjunctions where all variables/pipelines need to corroborate the hypothesis (i.e. regardless of which method one follows, the hypothesis is correct).

5.  Additional possible dimensions

No commitment is being made about the exhaustive nature of there only being three dimensions that specify the hypothesis scope. Other dimensions may exist that specify the scope of a hypothesis. For example, one might consider the pipeline dimension as two different dimensions. The first would consider the experimental pipeline dimension regarding all variables relating to the experimental setup to collect data, and the latter would be the analytical pipeline dimension regarding the data analysis of any given data snapshot. Another possible dimension is adding the number of situations or contexts under which the hypothesis is valid. For example, any restraint such as ‘in a vacuum’, ‘under the speed of light’, or ‘in healthy human adults' could be considered an additional dimension of the hypothesis. There is no objection to whether these should be additional dimensions of the hypothesis. However, as stated above, these usually follow from the boundary conditions of the theory.

6.  Specifying the scope versus assumptions

We envision that this framework can help hypothesis-makers formulate hypotheses (in research plans, registered reports, preregistrations etc.). Further, using this framework while formulating hypotheses can help distinguish between auxiliary hypotheses and parts of the scientific hypothesis being tested. When writing preregistrations, it can frequently occur that some step in the method has two alternatives (e.g. a preprocessing step), and there is not yet reason to choose one over the other, and the researcher needs to make a decision. These following scenarios are possible:

• 1. Narrow pipeline scope . The researcher evaluates the hypothesis with both pipeline variables (i.e. H holds for both p 1 and p 2 where p 1 and p 2 can be substituted with each other in the pipeline).
• 2. Broad pipeline scope. The researcher evaluates the hypothesis with both pipeline variables, and only one needs to be correct (i.e. H holds for either p 1 or p 2 where p 1 and p 2 can be substituted with each other in the pipeline). The result of this experiment may help motivate choosing either p 1 or p 2 in future studies.
• 3. Auxiliary hypothesis. Based on some reason (e.g. convention), the researcher assumes p 1 and evaluates H assuming p 1 is true.

Here we see that the same pipeline step can be part of either the auxiliary hypotheses or the pipeline scope. This distinction is important because if (3) is chosen, the decision becomes an assumption that is not explicitly tested by the hypothesis. Consequently, a researcher confident in the hypothesis may state that the auxiliary hypothesis p 1 was incorrect, and they should retest their hypothesis using different assumptions. In the cases where this decision is part of the pipeline scope, the hypothesis is intertwined with this decision, removing the eventual wiggle-room to reject auxiliary hypotheses that were assumed. Furthermore, starting with broader pipeline hypotheses that gradually narrow down can lead to a more well-motivated protocol for approaching the problem. Thus, this framework can help researchers while writing their hypotheses in, for example, preregistrations because they can consider when they are committing to a decision, assuming it, or when they should perhaps test a broader hypothesis with multiple possible options (discussed in more detail in §11 below).

7.  The reduction of scope in hypothesis space

Having established that different scopes of a hypothesis are possible, we now consider how the hypotheses change over time. In this section, we consider how the scope of the hypothesis develops ideally within science.

Consider a new research question. A large number of hypotheses are possible. Let us call this set of all possible hypotheses the hypothesis space . Hypotheses formulated within this space can be narrower or broader based on the dimensions discussed previously ( figure 3 ).

Example of hypothesis space. The hypothesis scope is expressed as cuboids in three dimensions (relationship ( R ), variable ( XY ), pipeline ( P )). The hypothesis space is the entire possible space within the three dimensions. Three hypotheses are shown in the hypothesis space (H 1 , H 2 , H 3 ). H 2 and H 3 are subsets of H 1 .

After the evaluation of the hypothesis with the scientific process, the hypothesis will be accepted or rejected. 9 The evaluation could be done through falsification or via verification, depending on the philosophy of science commitments. Thereafter, other narrower formulations of the hypothesis can be formulated by reducing the relationship, variable or pipeline scope. If a narrower hypothesis is accepted, more specific details about the subject matter are known, or a theory has been refined in greater detail. A narrower hypothesis will entail a more specific relationship, variable or pipeline detailed in the hypothesis. Consequently, hypotheses linked to each other in this way will become narrower over time along one or more dimensions. Importantly, considering that the conjunction of elements is narrower than single elements for pipelines and variables, this process of narrower hypotheses will lead to more general hypotheses (i.e. they have to be applied in all conditions and yield less flexibility when they do not apply). 10

Considering that the scopes of hypotheses were defined as sets above, some properties can be deduced from this framework about how narrower hypotheses relate to broader hypotheses. Let us consider three hypotheses (H 1 , H 2 , and H 3 ; figure 3 ). H 2 and H 3 are non-overlapping subsets of H 1 . Thus H 2 and H 3 are both narrower in scope than H 1 . Thus the following is correct:

• P1: If H 1 is false, then H 2 is false, and H 2 does not need to be evaluated.
• P2: If H 2 is true, then the broader H 1 is true, and H 1 does not need to be evaluated.
• P3: If H 1 is true and H 2 is false, some other hypothesis H 3 of similar scope to H 2 is possible.

For example, suppose H 1 is ‘there is a relationship between x and y ’, H 2 is ‘there is a positive relationship between x and y ’, and H 3 is ‘a negative relationship between x and y ’. In that case, it becomes apparent how each of these follows. 11 Logically, many deductions from set theory are possible but will not be explored here. Instead, we will discuss two additional consequences of hypothesis scopes: scientific novelty and applications for the researcher who formulates a hypothesis.

P1–P3 have been formulated as hypotheses being true or false. In practice, hypotheses are likely evaluated probabilistically (e.g. ‘H 1 is likely’ or ‘there is evidence in support of H 1 ’). In these cases, P1–P3 can be rephrased to account for this by substituting true/false with statements relating to evidence. For example, P2 could read: ‘If there is evidence in support of H 2 , then there is evidence in support of H 1 , and H 1 does not need to be evaluated’.

8.  Scientific novelty as the reduction of scope

Novelty is a key concept that repeatedly occurs in multiple aspects of the scientific enterprise, from funding to publishing [ 55 ]. Generally, scientific progress establishes novel results based on some new hypothesis. Consequently, the new hypothesis for the novel results must be narrower than previously established knowledge (i.e. the size of the scopes is reduced). Otherwise, the result is trivial and already known (see P2 above). Thus, scientific work is novel if the scientific process produces a result based on hypotheses with either a smaller | R |, | XY |, or | P | compared to previous work.

This framework of dimensions of the scope of a hypothesis helps to demarcate when a hypothesis and the subsequent result are novel. If previous studies have established evidence for R 1 (e.g. there is a positive relationship between x and y ), a hypothesis will be novel if and only if it is narrower than R 1 . Thus, if R 2 is narrower in scope than R 1 (i.e. | R 2 | < | R 1 |), R 2 is a novel hypothesis.

Consider the following example. Study 1 hypothesizes, ‘There is a positive relationship between x and y ’. It identifies a linear relationship of 0.6. Next, Study 2 hypothesizes, ‘There is a specific linear relationship between x and y that is 0.6’. Study 2 also identifies the relationship of 0.6. Since this was a narrower hypothesis, Study 2 is novel despite the same result. Frequently, researchers claim that they are the first to demonstrate a relationship. Being the first to demonstrate a relationship is not the final measure of novelty. Having a narrower hypothesis than previous researchers is a sign of novelty as it further reduces the hypothesis space.

Finally, it should be noted that novelty is not the only objective of scientific work. Other attributes, such as improving the certainty of a current hypothesis (e.g. through replications), should not be overlooked. Additional scientific explanations and improved theories are other aspects. Additionally, this definition of novelty relating to hypothesis scope does not exclude other types of novelty (e.g. new theories or paradigms).

9.  How broad should a hypothesis be?

Given the previous section, it is elusive to conclude that the hypothesis should be as narrow as possible as it entails maximal knowledge gain and scientific novelty when formulating hypotheses. Indeed, many who advocate for daring or risky tests seem to hold this opinion. For example, Meehl [ 46 ] argues that we should evaluate theories based on point (or interval) prediction, which would be compatible with very narrow versions of relationships. We do not necessarily think that this is the most fruitful approach. In this section, we argue that hypotheses should aim to be narrower than current knowledge , but too narrow may be problematic .

Let us consider the idea of confirmatory analyses. These studies will frequently keep the previous hypothesis scopes regarding P and XY but aim to become more specific regarding R (i.e. using the same method and the same variables to detect a more specific relationship). A very daring or narrow hypothesis is to minimize R to include the fewest possible relationships. However, it becomes apparent that simply pursuing specificness or daringness is insufficient for selecting relevant hypotheses. Consider a hypothetical scenario where a researcher believes virtual reality use leads people to overestimate the amount of exercise they have done. If unaware of previous studies on this project, an apt hypothesis is perhaps ‘increased virtual reality usage correlates with a less accuracy of reported exercise performed’ (i.e. R is broad). However, a more specific and more daring hypothesis would be to specify the relationship further. Thus, despite not knowing if there is a relationship at all, a more daring hypothesis could be: ‘for every 1 h of virtual reality usage, there will be, on average, a 0.5% decrease in the accuracy of reported exercise performed’ (i.e. R is narrow). We believe it would be better to establish the broader hypothesis in any scenario here for the first experiment. Otherwise, if we fail to confirm the more specific formulation, we could reformulate another equally narrow relative to the broader hypothesis. This process of tweaking a daring hypothesis could be pursued ad infinitum . Such a situation will neither quickly identify the true hypothesis nor effectively use limited research resources.

By first discounting a broader hypothesis that there is no relationship, it will automatically discard all more specific formulations of that relationship in the hypothesis space. Returning to figure 3 , it will be better to establish H 1 before attempting H 2 or H 3 to ensure the correct area in the hypothesis space is being investigated. To provide an analogy: when looking for a needle among hay, first identify which farm it is at, then which barn, then which haystack, then which part of the haystack it is at before we start picking up individual pieces of hay. Thus, it is preferable for both pragmatic and cost-of-resource reasons to formulate sufficiently broad hypotheses to navigate the hypothesis space effectively.

Conversely, formulating too broad a relationship scope in a hypothesis when we already have evidence for narrower scope would be superfluous research (unless the evidence has been called into question by, for example, not being replicated). If multiple studies have supported the hypothesis ‘there is a 20-fold decrease in mortality after taking some medication M’, it would be unnecessary to ask, ‘Does M have any effect?’.

Our conclusion is that the appropriate scope of a hypothesis, and its three dimensions, follow a Goldilocks-like principle where too broad is superfluous and not novel, while too narrow is unnecessary or wasteful. Considering the scope of one's hypothesis and how it relates to previous hypotheses' scopes ensures one is asking appropriate questions.

Finally, there has been a recent trend in psychology that hypotheses should be formal [ 38 , 56 – 60 ]. Formal theories are precise since they are mathematical formulations entailing that their interpretations are clear (non-ambiguous) compared to linguistic theories. However, this literature on formal theories often refers to ‘precise predictions’ and ‘risky testing’ while frequently referencing Meehl, who advocates for narrow hypotheses (e.g. [ 38 , 56 , 59 ]). While perhaps not intended by any of the proponents, one interpretation of some of these positions is that hypotheses derived from formal theories will be narrow hypotheses (i.e. the quality of being ‘precise’ can mean narrow hypotheses with risky tests and non-ambiguous interpretations simultaneously). However, the benefit from the clarity (non-ambiguity) that formal theories/hypotheses bring also applies to broad formal hypotheses as well. They can include explicit but formalized versions of uncertain relationships, multiple possible pipelines, and large sets of variables. For example, a broad formal hypothesis can contain a hyperparameter that controls which distribution the data fit (broad relationship scope), or a variable could represent a set of formalized explicit pipelines (broad pipeline scope) that will be tested. In each of these instances, it is possible to formalize non-ambiguous broad hypotheses from broad formal theories that do not yet have any justification for being overly narrow. In sum, our argumentation here stating that hypotheses should not be too narrow is not an argument against formal theories but rather that hypotheses (derived from formal theories) do not necessarily have to be narrow.

10.  Other approaches to categorizing hypotheses

The framework we present here is a way of categorizing hypotheses into (at least) three dimensions regarding the hypothesis scope, which we believe is accessible to researchers and help link scientific work over time while also trying to remain neutral with regard to a specific philosophy of science. Our proposal does not aim to be antagonistic or necessarily contradict other categorizing schemes—but we believe that our framework provides benefits.

One recent categorization scheme is the Theoretical (T), Auxiliary (A), Statistical (S) and Inferential (I) assumption model (together becoming the TASI model) [ 61 , 62 ]. Briefly, this model considers theory to generate theoretical hypotheses. To translate from theoretical unobservable terms (e.g. personality, anxiety, mass), auxiliary assumptions are needed to generate an empirical hypothesis. Statistical assumptions are often needed to test the empirical hypothesis (e.g. what is the distribution, is it skewed or not) [ 61 , 62 ]. Finally, additional inferential assumptions are needed to generalize to a larger population (e.g. was there a random and independent sampling from defined populations). The TASI model is insightful and helpful in highlighting the distance between a theory and the observation that would corroborate/contradict it. Part of its utility is to bring auxiliary hypotheses into the foreground, to improve comparisons between studies and improve theory-based interventions [ 63 , 64 ].

We do agree with the importance of being aware of or stating the auxiliary hypotheses, but there are some differences between the frameworks. First, the number of auxiliary assumptions in TASI can be several hundred [ 62 ], whereas our framework will consider some of them as part of the pipeline dimension. Consider the following four assumptions: ‘the inter-stimulus interval is between 2000 ms and 3000 ms', ‘the data will be z-transformed’, ‘subjects will perform correctly’, and ‘the measurements were valid’. According to the TASI model, all these will be classified similarly as auxiliary assumptions. Contrarily, within our framework, it is possible to consider the first two as part of the pipeline dimension and the latter two as auxiliary assumptions, and consequently, the first two become integrated as part of the hypothesis being tested and the latter two auxiliary assumptions. A second difference between the frameworks relates to non-theoretical studies (convenience, applied or atheoretical). Our framework allows for the possibility that the hypothesis space generated by theoretical and convenience studies can interact and inform each other within the same framework . Contrarily, in TASI, the theory assumptions no longer apply, and a different type of hypothesis model is needed; these assumptions must be replaced by another group of assumptions (where ‘substantive application assumptions' replace the T and the A, becoming SSI) [ 61 ]. Finally, part of our rationale for our framework is to be able to link and track hypotheses and hypothesis development together over time, so our classification scheme has different utility.

Another approach which has some similar utility to this framework is theory construction methodology (TCM) [ 57 ]. The similarity here is that TCM aims to be a practical guide to improve theory-making in psychology. It is an iterative process which relates theory, phenomena and data. Here hypotheses are not an explicit part of the model. However, what is designated as ‘proto theory’ could be considered a hypothesis in our framework as they are a product of abduction, shaping the theory space. Alternatively, what is deduced to evaluate the theory can also be considered a hypothesis. We consider both possible and that our framework can integrate with these two steps, especially since TCM does not have clear guidelines for how to do each step.

11.  From theory to practice: implementing this framework

We believe that many practising researchers can relate to many aspects of this framework. But, how can a researcher translate the above theoretical framework to their work? The utility of this framework lies in bringing these three scopes of a hypothesis together and explaining how each can be reduced. We believe researchers can use this framework to describe their current practices more clearly. Here we discuss how it can be helpful for researchers when formulating, planning, preregistering, and discussing the evaluation of their scientific hypotheses. These practical implications are brief, and future work can expand on the connection between the full interaction between hypothesis space and scope. Furthermore, both authors have the most experience in cognitive neuroscience, and some of the practical implications may revolve around this type of research and may not apply equally to other fields.

11.1. Helping to form hypotheses

Abduction, according to Peirce, is a hypothesis-making exercise [ 22 , 26 – 28 ]. Given some observations, a general testable explanation of the phenomena is formed. However, when making the hypothesis, this statement will have a scope (either explicitly or implicitly). Using our framework, the scope can become explicit. The hypothesis-maker can start with ‘The variables XY have a relationship R with pipeline P ’ as a scaffold to form the hypothesis. From here, the hypothesis-maker can ‘fill in the blanks’, explicitly adding each of the scopes. Thus, when making a hypothesis via abduction and using our framework, the hypothesis will have an explicit scope when it is made. By doing this, there is less chance that a formulated hypothesis is unclear, ambiguous, and needs amending at a later stage.

11.2. Assisting to clearly state hypotheses

A hypothesis is not just formulated but also communicated. Hypotheses are stated in funding applications, preregistrations, registered reports, and academic articles. Further, preregistered hypotheses are often omitted or changed in the final article [ 11 ], and hypotheses are not always explicitly stated in articles [ 12 ]. How can this framework help to make better hypotheses? Similar to the previous point, filling in the details of ‘The variables XY have a relationship R with pipeline P ’ is an explicit way to communicate the hypothesis. Thinking about each of these dimensions should entail an appropriate explicit scope and, hopefully, less variation between preregistered and reported hypotheses. The hypothesis does not need to be a single sentence, and details of XY and P will often be developed in the methods section of the text. However, using this template as a starting point can help ensure the hypothesis is stated, and the scope of all three dimensions has been communicated.

11.3. Helping to promote explicit and broad hypotheses instead of vague hypotheses

There is an important distinction between vague hypotheses and broad hypotheses, and this framework can help demarcate between them. A vague statement would be: ‘We will quantify depression in patients after treatment’. Here there is uncertainty relating to how the researcher will go about doing the experiment (i.e. how will depression be quantified?). However, a broad statement can be uncertain, but the uncertainty is part of the hypothesis: ‘Two different mood scales (S 1 or S 2 ) will be given to patients and test if only one (or both) changed after treatment’. This latter statement is transparently saying ‘S 1 or S 2 ’ is part of a broad hypothesis—the uncertainty is whether the two different scales are quantifying the same construct. We keep this uncertainty within the broad hypothesis, which will get evaluated, whereas a vague hypothesis has uncertainty as part of the interpretation of the hypothesis. This framework can be used when formulating hypotheses to help be broad (where needed) but not vague.

11.4. Which hypothesis should be chosen?

When considering the appropriate scope above, we argued for a Goldilocks-like principle of determining the hypothesis that is not too broad or too narrow. However, when writing, for example, a preregistration, how does one identify this sweet spot? There is no easy or definite universal answer to this question. However, one possible way is first to identify the XY , R , and P of previous hypotheses. From here, identify what a non-trivial step is to improve our knowledge of the research area. So, for example, could you be more specific about the exact nature of the relationship between the variables? Does the pipeline correspond to today's scientific standards, or were some suboptimal decisions made? Is there another population that you think the previous result also applies to? Do you think that maybe a more specific construct or subpopulation might explain the previous result? Could slightly different constructs (perhaps easier to quantify) be used to obtain a similar relationship? Are there even more constructs to which this relationship should apply simultaneously? Are you certain of the direction of the relationship? Answering affirmatively to any of these questions will likely make a hypothesis narrower and connect to previous research while being clear and explicit. Moreover, depending on the research question, answering any of these may be sufficiently narrow to be a non-trivial innovation. However, there are many other ways to make a hypothesis narrower than these guiding questions.

11.5. The confirmatory–exploratory continuum

Research is often dichotomized into confirmatory (testing a hypothesis) or exploratory (without a priori hypotheses). With this framework, researchers can consider how their research acts on some hypothesis space. Confirmatory and exploratory work has been defined in terms of how each interacts with the researcher's degrees of freedom (where confirmatory aims to reduce while exploratory utilizes them [ 30 ]). Both broad confirmatory and narrow exploratory research are possible using this definition and possible within this framework. How research interacts with the hypothesis space helps demarcate it. For example, if a hypothesis reduces the scope, it becomes more confirmatory, and trying to understand data given the current scope would be more exploratory work. This further could help demarcate when exploration is useful. Future theoretical work can detail how different types of research impact the hypothesis space in more detail.

11.6. Understanding when multiverse analyses are needed

Researchers writing a preregistration may face many degrees of freedom they have to choose from, and different researchers may motivate different choices. If, when writing such a preregistration, there appears to be little evidential support for certain degrees of freedom over others, the researcher is left with the option to either make more auxiliary assumptions or identify when an investigation into the pipeline scope is necessary by conducting a multiverse analysis that tests the impact of the different degrees of freedom on the result (see [ 8 ]). Thus, when applying this framework to explicitly state what pipeline variables are part of the hypothesis or an auxiliary assumption, the researcher can identify when it might be appropriate to conduct a multiverse analysis because they are having difficulty formulating hypotheses.

11.7. Describing novelty

Academic journals and research funders often ask for novelty, but the term ‘novelty’ can be vague and open to various interpretations [ 55 ]. This framework can be used to help justify the novelty of research. For example, consider a scenario where a previous study has established a psychological construct (e.g. well-being) that correlates with a certain outcome measure (e.g. long-term positive health outcomes). This framework can be used to explicitly justify novelty by (i) providing a more precise understanding of the relationship (e.g. linear or linear–plateau) or (ii) identifying more specific variables related to well-being or health outcomes. Stating how some research is novel is clearer than merely stating that the work is novel. This practice might even help journals and funders identify what type of novelty they would like to reward. In sum, this framework can help identify and articulate how research is novel.

11.8. Help to identify when standardization of pipelines is beneficial or problematic to a field

Many consider standardization in a field to be important for ensuring the comparability of results. Standardization of methods and tools entails that the pipeline P is identical (or at least very similar) across studies. However, in such cases, the standardized pipeline becomes an auxiliary assumption representing all possible pipelines. Therefore, while standardized pipelines have their benefits, this assumption becomes broader without validating (e.g. via multiverse analysis) which pipelines a standardized P represents. In summary, because this framework helps distinguish between auxiliary assumptions and explicit parts of the hypothesis and identifies when a multiverse analysis is needed, it can help determine when standardizations of pipelines are representative (narrower hypotheses) or assumptive (broader hypotheses).

12.  Conclusion

Here, we have argued that the scope of a hypothesis is made up of three dimensions: the relationship ( R ), variable ( XY ) and pipeline ( P ) selection. Along each of these dimensions, the scope can vary. Different types of scientific enterprises will often have hypotheses that vary the size of the scopes. We have argued that this focus on the scope of the hypothesis along these dimensions helps the hypothesis-maker formulate their hypotheses for preregistrations while also helping demarcate auxiliary hypotheses (assumed to be true) from the hypothesis (those being evaluated during the scientific process).

Hypotheses are an essential part of the scientific process. Considering what type of hypothesis is sufficient or relevant is an essential job of the researcher that we think has been overlooked. We hope this work promotes an understanding of what a hypothesis is and how its formulation and reduction in scope is an integral part of scientific progress. We hope it also helps clarify how broad hypotheses need not be vague or inappropriate.

Finally, we applied this idea of scopes to scientific progress and considered how to formulate an appropriate hypothesis. We have also listed several ways researchers can practically implement this framework today. However, there are other practicalities of this framework that future work should explore. For example, it could be used to differentiate and demarcate different scientific contributions (e.g. confirmatory studies, exploration studies, validation studies) with how their hypotheses interact with the different dimensions of the hypothesis space. Further, linking hypotheses over time within this framework can be a foundation for open hypothesis-making by promoting explicit links to previous work and detailing the reduction of the hypothesis space. This framework helps quantify the contribution to the hypothesis space of different studies and helps clarify what aspects of hypotheses can be relevant at different times.

Acknowledgements

We thank Filip Gedin, Kristoffer Sundberg, Jens Fust, and James Steele for valuable feedback on earlier versions of this article. We also thank Mark Rubin and an unnamed reviewer for valuable comments that have improved the article.

1 While this is our intention, we cannot claim that every theory has been accommodated.

2 Similar requirements of science being able to evaluate the hypothesis can be found in pragmatism [ 22 ], logical positivism [ 23 ] and falsification [ 24 ].

3 Although when making inferences about a failed evaluation of a scientific hypothesis it is possible, due to underdetermination, to reject the auxiliary hypothesis instead of rejecting the hypothesis. However, that rejection occurs at a later inference stage. The evaluation using the scientific method aims to test the scientific hypothesis, not the auxiliary assumptions.

4 Although some have argued that this practice is not as problematic or questionable (see [ 34 , 35 ]).

5 Alternatively, theories sometimes expand their boundary conditions. A theory that was previously about ‘humans' can be used with a more inclusive boundary condition. Thus it is possible for the hypothesis-maker to use a theory about humans (decision making) and expand it to fruit flies or plants (see [ 43 ]).

6 A similarity exists here with Popper, where he uses set theory in a similar way to compare theories (not hypotheses). Popper also discusses how theories with overlapping sets but neither is a subset are also comparable (see [ 24 , §§32–34]). We do not exclude this possibility but can require additional assumptions.

7 When this could be unclear, we place the element within quotation marks.

8 Here, we have assumed that there is no interaction between these variables in variable selection. If an interaction between x 1 and x 2 is hypothesized, this should be viewed as a different variable compared to ‘ x 1 or x 2 ’. The motivation behind this is because the hypothesis ‘ x 1 or x 2 ’ is not a superset of the interaction (i.e. ‘ x 1 or x 2 ’ is not necessarily true when the interaction is true). The interaction should, in this case, be considered a third variable (e.g. I( x 1 , x 2 )) and the hypothesis ‘ x 1 or x 2 or I( x 1 , x 2 )’ is broader than ‘ x 1 or x 2 ’.

9 Or possibly ambiguous or inconclusive.

10 This formulation of scope is compatible with different frameworks from the philosophy of science. For example, by narrowing the scope would in a Popperian terminology mean prohibiting more basic statements (thus a narrower hypothesis has a higher degree of falsifiability). The reduction of scope in the relational dimension would in Popperian terminology mean increase in precision (e.g. a circle is more precise than an ellipse since circles are a subset of possible ellipses), whereas reduction in variable selection and pipeline dimension would mean increase universality (e.g. ‘all heavenly bodies' is more universal than just ‘planets') [ 24 ]. For Meehl the reduction of the relationship dimension would amount to decreasing the relative tolerance of a theory to the Spielraum [ 46 ] .

11 If there is no relationship between x and y , we do not need to test if there is a positive relationship. If we know there is a positive relationship between x and y , we do not need to test if there is a relationship. If we know there is a relationship but there is not a positive relationship, then it is possible that they have a negative relationship.

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Declaration of ai use.

We have not used AI-assisted technologies in creating this article.

Authors' contributions

W.H.T.: conceptualization, investigation, writing—original draft, writing—review and editing; S.S.: investigation, writing—original draft, writing—review and editing.

Both authors gave final approval for publication and agreed to be held accountable for the work performed therein.

Conflict of interest declaration

We declare we have no competing interests.

We received no funding for this study.

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cohesion hypothesis

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cohesion hypothesis , in botany , a generally accepted explanation of the rise of sap in vascular plants by means of intermolecular attractions. Calculation and experiment indicate that the forces of cohesion between water molecules and the forces of adhesion between water molecules and the walls of the xylem vessel cells are sufficient to confer on thin columns of water a tensile strength of at least 30 atmospheres (440 pounds per square inch). This is high enough to permit a thin column of water to be lifted to the top of any tree without breaking the column.

The cohesion of water explains only maintenance of the sap column; the explanation for the upward movement of the water is accounted for by a mechanism, called transpiration pull , that involves the evaporation of water from leaves . Thus, the explanation for the upward movement of sap in trees and other plants is also called the transpiration-cohesion hypothesis . It accounts for the observed rise of sap and agrees with observed tensions (pressures below zero, or negative pressures) in plant stems and gradients of tension between upper and lower parts of the same plant. The stability of water columns in wind-tossed plants is more difficult to understand. It is possible only because the water is enclosed in millions of small compartments ( tracheids and vessels ).