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Letter Templates

Job Application Letter With Reference To Newspaper Ad

Applying for a job can be a daunting task, especially when you have to write a job application letter. However, with the right approach and guidance, you can make the process easier and increase your chances of being hired. In this article, we will discuss the job application letter with reference to a newspaper ad and provide you with examples and tips to help you write an effective and impressive letter.

A job application letter is a formal letter that you send to an employer in response to a job advertisement. The purpose of the letter is to showcase your skills, experience, and qualifications and convince the employer that you are the best candidate for the job. The letter should be concise, informative, and tailored to the requirements of the job.

When writing a job application letter with reference to a newspaper ad, you should start by reading the ad carefully and understanding the job requirements. Then, you should tailor your letter to match the requirements and highlight your relevant skills and experience. You should also mention where you saw the ad and express your interest in the job. The letter should be well-written, error-free, and easy to read.

Readers can find examples of job application letters with reference to a newspaper ad and edit them as needed to fit their specific situation. These examples will help you understand the structure and tone of the letter and give you an idea of what to include in your own letter.

Example 1: Applying for a Marketing Manager Position

Dear Hiring Manager,

I am writing to express my interest in the Marketing Manager position advertised in the Daily News on June 1st. With over 5 years of experience in marketing and a Bachelor’s degree in Marketing, I believe I possess the skills and qualifications required for this position.

In my current position as a Marketing Specialist at XYZ Inc, I have successfully developed and implemented marketing campaigns that have increased sales by 20%. I am also proficient in digital marketing, social media marketing, and market research.

I am excited about the opportunity to join your team and contribute to your company’s growth. Please find my resume attached for your review. Thank you for considering my application.

Frequently Asked Questions

Q: what is a job application letter.

A job application letter is a formal letter that you send to an employer in response to a job advertisement. The purpose of the letter is to showcase your skills, experience, and qualifications and convince the employer that you are the best candidate for the job.

Q: How do I start a job application letter?

You should start by reading the job advertisement carefully and understanding the requirements of the job. Then, you should tailor your letter to match the requirements and highlight your relevant skills and experience. You can start the letter with a professional greeting and introduce yourself and express your interest in the job.

Q: What should I include in a job application letter?

You should include your contact information, the employer’s contact information, a professional greeting, an introduction, a body that highlights your skills and experience, a closing that expresses your interest in the job, and a complimentary close and your signature. You should also proofread the letter for errors and make sure it is easy to read.

Q: How do I tailor my job application letter to the job requirements?

You can tailor your job application letter to the job requirements by understanding the requirements and highlighting your relevant skills and experience. You can also research the company and its culture and mention why you would be a good fit for the job and the company.

Q: How long should a job application letter be?

A job application letter should be concise, informative, and easy to read. It should be no longer than one page and should highlight your skills and experience that are relevant to the job.

Q: Should I attach my resume to my job application letter?

Yes, you should attach your resume to your job application letter. Your resume should complement your letter and provide more details about your skills and experience.

Q: How do I address my job application letter if I don’t know the name of the hiring manager?

You can address your job application letter to “Dear Hiring Manager” or “To Whom It May Concern”. However, it is recommended that you do some research and try to find out the name of the hiring manager to personalize your letter and make it more effective.

Writing a job application letter with reference to a newspaper ad requires careful attention to detail and a tailored approach. By following the tips and examples provided in this article, you can write an effective and impressive letter that increases your chances of being hired. Remember to proofread your letter for errors and make sure it is easy to read and well-structured.

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Solicited Application Letters: Writing an Effective Letter

Solicited Application Letters are written in response to a job advertisement or a vacancy announcement. This is a common way of applying for jobs, especially when applying through job search websites or company career portals. In this article, we will focus on how to write an effective Solicited Application Letter that will help you increase your chances of landing a job.

Definition of Solicited Application Letters

Solicited Application Letters are also known as cover letters or job application letters. These are documents that are sent with your resume to introduce yourself and explain the reasons why you are a good fit for the position you are applying for.  They are called “solicited” because you are submitting an application in response to a specific job posting or advertisement.

Importance of Writing an Effective Letter

The main purpose of a Solicited Application Letter is to convince the employer that you are the right candidate for the job. Writing an effective letter is crucial because it gives you an opportunity to make a good first impression and stand out from other applicants. An effective Solicited Application Letter should be tailored to the job requirements, highlight your skills and experience, and demonstrate your enthusiasm for the job.

Writing an effective Solicited Application Letter is essential when applying for jobs. It can be the key to getting your foot in the door and securing an interview. Follow these tips to write a winning letter and increase your chances of landing your dream job.

Understanding the Job Description

When it comes to writing an effective solicited application letter, the first and most crucial step is to gain a clear understanding of the job description. This involves analyzing the job requirements and researching the company to tailor your letter to their specific needs.

Analyzing the Job Requirements

Analyzing the job requirements means going beyond just reading the job title and basic duties. It requires a thorough understanding of the skills, experience, and qualifications the employer is looking for. Take note of any specific qualifications or certifications required, as well as any desired soft skills like communication or teamwork ability.

Once you have a clear understanding of the job requirements, you can use this information to highlight your most relevant skills and experience in your application letter. This will show the employer that you have what it takes to excel in the role and set you apart from other candidates.

Researching the Company

In addition to understanding the job requirements, it’s important to research the company you’re applying to. This shows that you’re genuinely interested in the role and have taken the time to learn about the company and its values. Start by browsing the company’s website, social media pages, and any recent news articles about the company.

Focus on their mission, values, and culture, and think about how you can align your skills and experience with their goals. If the company has any current projects or initiatives, mention them in your application letter and explain how you could contribute to their success.

Understanding the job description and researching the company is essential for writing an effective solicited application letter. By tailoring your letter to the employer’s specific needs and demonstrating your interest in the company, you’ll have a better chance of standing out and landing your dream job.

Crafting a Compelling Opening Paragraph

An effective solicited application letter begins with a compelling opening paragraph that immediately captures the reader’s attention. This opening paragraph serves as your chance to make a good first impression and set the tone for the rest of your letter. A strong opening can compel the hiring manager to read on, while a weak one can lead to your application being overlooked.

Making a Good First Impression

Your opening paragraph needs to be interesting, engaging, and impactful. You want to start with a hook that draws the reader in and makes them want to keep reading. This might be a statement that highlights your skills or experience, a personal anecdote that relates to the job, or a powerful quote that sets the tone. Whatever approach you take, it should be tailored to the company and position you’re applying to.

Beyond being interesting, your opening paragraph also needs to be well-written and professional. This means using correct grammar, punctuation, and spelling, as well as avoiding slang or overly casual language. Remember, you want to convey that you’re a serious candidate who takes the application process seriously.

Avoiding Common Opening Mistakes

Along with crafting a great opening, it’s also important to avoid some common mistakes that can weaken your application. One of the biggest mistakes is starting off with a generic or vague statement, such as “I am writing to express my interest in the open position.” This type of opening does little to capture the reader’s attention or differentiate you from other applicants.

Another mistake is being too self-promotional or boastful in your opening, such as saying “I am the best candidate for the job.” While it’s important to highlight your strengths, it’s also important to remain humble and let your qualifications speak for themselves.

Finally, avoid opening paragraphs that are too long or rambling. Remember that the hiring manager likely has many applications to review, so you want to be concise and get to the point quickly. Aim for a paragraph that is no more than five sentences and effectively communicates your interest in the position and qualifications.

By crafting a compelling opening paragraph that makes a good first impression and avoiding common mistakes, you can increase your chances of catching the hiring manager’s attention and moving on to the next stage of the application process.

Highlighting Relevant Skills and Qualifications

One of the most important parts of a solicited application letter is showcasing your relevant skills and qualifications. This helps the employer quickly see how you match their needs and requirements. But it’s not just about listing your qualifications; it’s also important to highlight your experience and accomplishments.

Showcasing Experience and Accomplishments

Employers want to know what you have done in the past that makes you a good fit for their job. This is where highlighting your experience and accomplishments can make a big difference. Instead of just stating your job title and responsibilities, think about what you have achieved in each role.

For instance, instead of saying “Managed social media accounts”, you could say “Increased social media engagement by 50% through targeted content and strategic advertising campaigns”. This not only shows your experience, but also highlights your accomplishments and how you can bring value to the company.

Aligning Your Experience with the Job Requirements

When highlighting your experience and accomplishments, it’s important to align them with the job requirements. This means taking the time to understand what the employer is looking for and tailoring your application letter to match those needs.

To do this, carefully read the job posting and identify the key skills and qualifications they are looking for. Then, use specific examples from your past experience to show how you meet those requirements.

For instance, if the job posting emphasizes the need for strong communication skills, highlight your experience in public speaking or your success in leading a team through a difficult project. This shows the employer that you not only have the required skills, but also understand their importance in the job.

Showcasing your relevant skills, experience, and accomplishments is crucial in a solicited application letter. By aligning your experience with the job requirements, you can demonstrate your value to the employer and increase your chances of landing an interview.

Articulating Your Value Proposition

One of the most critical components of a solicited application letter is to communicate your value proposition effectively. A value proposition is the unique value that you bring to the table as a candidate, which should set you apart from the competition.

When it comes to communicating your unique selling points, it is essential to keep in mind that your letter should be tailored to the job description and the company’s needs. You may have several talents and qualifications, but it’s necessary to highlight the ones that are most relevant to the position.

To begin with, it’s important to do your research about the company and the role. Review the job posting and the company’s website, paying particular attention to the requirements and the company’s mission and values. This information can provide valuable insights into the types of skills and qualities that the company is looking for in its employees.

Once you have a good understanding of the role and what the company is looking for, you can start articulating your value proposition. Here are some tips to help you get started:

Focus on results: Instead of just listing your skills, focus on the results you’ve achieved. For example, instead of saying that you have excellent communication skills, provide an example of where you have communicated effectively, improving overall team performance.

Communicate your passion: Hiring managers often want to know that you are genuinely interested in the company and the job. Highlight your passion for the industry or the role and demonstrate how your enthusiasm translates into your work.

Show your problem-solving skills: Companies are always looking for employees who are great problem-solvers. Provide examples that demonstrate how you have solved problems in the past, showcasing your skills and experience in tackling complex issues.

Highlight your unique skills: Identify the skills, qualities, and experiences that set you apart from your competition. These unique selling points may include your specific knowledge of the industry, your certifications, or your ability to apply a unique perspective to problem-solving.

The goal is to use your value proposition to demonstrate the value that you can bring to the role. It’s essential to show the hiring manager how you can make a meaningful impact on the company and the team. By following these tips and using specific examples from your experience, you can articulate your value proposition effectively, making a compelling case for your candidacy.

Tailoring the Letter to the Specific Job

When writing a solicited application letter, it is important to customize it to the specific company and position you are applying for. This shows that you have done your research and are truly interested in the job.

To customize your letter, start by researching the company. Look at their website, their LinkedIn page, and any other resources that might give you an idea of their culture and values. Then, find a connection between those values and your own experience, skills, and achievements. This will show the employer that you understand their needs and are a good fit for the position.

Next, review the job description and make note of any keywords or phrases that stand out. These might include specific skills, certifications, or experiences that are required or preferred. Use these keywords in your letter to show that you have the qualifications they are looking for.

For example, if the job description mentions experience in project management, be sure to highlight your experience in managing complex projects. If the job requires a certain certification, mention that you have obtained it. By using these keywords, you show that you are not just sending a generic letter, but one that specifically addresses the needs of the job.

When customizing your letter, remember to keep it professional and concise. Don’t go into too much detail about your personal life or unrelated experiences. Stick to the relevant qualifications that make you a strong candidate for the position.

Customizing your letter shows that you are invested in the job and have taken the time to research and understand the company and position. This can set you apart from other applicants and increase your chances of getting an interview. Remember to use keywords from the job description and highlight your relevant experience and skills to create an effective solicited application letter.

Writing an Engaging Body Paragraph

One of the most crucial parts of a solicited application letter is the body paragraph. This is where you can showcase your understanding of the company culture and describe how you fit into the company’s goals.

When writing your application letter’s body paragraph, it is essential to research the company thoroughly. This includes reviewing the company’s values, mission statement, and social media to gain further insight into their overall culture. Once you have a solid understanding of the company’s personality, you can tailor your language to align with their communication style.

However, understanding the company culture is not enough. You need to demonstrate how you would fit into the company’s goals. One way to do this is to relate your skills and experiences to the company’s current challenges. You can highlight your problem-solving skills and provide concrete examples of how you have achieved similar goals in your previous experience.

Another way to describe how you fit into the company’s goals is by discussing your long-term career objectives. Suppose the company’s goals resonate with your passions and career trajectory. In that case, you can express your desire to grow with the company and contribute to their success in the future.

Additionally, you can share what excites you about the company’s mission and how working for the organization aligns with your core values. By doing so, you can communicate your genuine interest in the company and the industry.

Writing an engaging body paragraph in a solicited application letter requires research, strategic language, and a personalized approach. A well-crafted body paragraph can demonstrate your understanding of the company culture and how you fit into their goals, leading to a more effective application.

Overcoming Weaknesses or Gaps in Experience

During the job application process, it’s common for applicants to encounter weaknesses or gaps in their experience or resume that may hinder their chances of getting the job they desire. It’s important to be proactive and address these challenges effectively to increase your chances of success.

Addressing Challenges in Your Background or Resume

One of the first steps in addressing any challenges in your background or resume is to identify them. Take an honest look at your qualifications and work experience, and try to anticipate any possible objections that a potential employer might have. For example, if you don’t have a required certification, or if you have a gap in employment, be prepared to address these issues in your application.

Once you’ve identified any potential challenges, it’s important to be honest about them in your application. Don’t try to hide or downplay your weaknesses or gaps in experience. Instead, acknowledge them and provide solutions for overcoming them.

Providing Solutions to These Challenges

When addressing weaknesses or gaps in your experience or resume, it’s important to be proactive and provide potential solutions to any issues. Here are some strategies to consider:

Highlight transferable skills.  If you lack experience in a particular area, focus on transferable skills that you possess that are relevant to the job you’re applying for. For example, if you’re applying for a marketing position but lack direct marketing experience, highlight skills like communication, creativity, and analytical thinking that are relevant to the role.

Seek out training or education opportunities.  If you lack a required certification or degree, consider seeking out opportunities to complete the necessary training or education. You may need to invest time or money into this, but it can pay off in the long run by making you a more competitive candidate.

Volunteer or intern.  If you have a gap in employment, consider volunteering or interning to gain experience and fill the gap. This can demonstrate your commitment and work ethic to potential employers, and can also provide valuable experience that can help you in your future career.

Address any concerns head-on.  If you anticipate that an employer may have concerns about a particular issue in your background or resume, be proactive and address it directly in your application. For example, if you were let go from a previous job, provide context and explain what you learned from the experience.

By being proactive and providing solutions for any weaknesses or gaps in your experience or resume, you can increase your chances of success in the job application process. With a thoughtful and strategic approach, you can overcome any challenges and demonstrate your value as a candidate.

Crafting an Effective Closing Paragraph

A strong closing paragraph can leave a lasting impression on your potential employer. To maximize your chances of success, you should express enthusiasm and interest in the role, while also providing your contact information and requesting follow-up.

Expressing Enthusiasm and Interest in the Role

Before wrapping up your solicited application letter, take the time to reiterate your enthusiasm and genuine interest in the role. Highlight your qualifications and how they align with the job requirements. Be specific about the skills and experience you have to offer, and how they can benefit the organization.

Use positive, welcoming language to convey your eagerness. For example, you might say: “I am excited about the opportunity to join the team and contribute my skills and experience to the organization’s mission. I have been following the company’s growth and am impressed by its commitment to innovation and excellence.”

By expressing genuine interest and enthusiasm for the role and the company, you can leave a positive impression on the reader and increase your chances of being invited for an interview.

Providing Contact Information and Request for Follow-up

Your closing paragraph should include your contact information, such as your phone number and email address. Make it easy for the employer to get in touch with you by providing clear and accurate contact details.

In addition, consider including a request for follow-up. Express your interest in hearing back from the employer, and suggest a timeline for a potential response. For example, you might say: “I would be grateful for the opportunity to discuss my qualifications further in an interview at your convenience. I will follow up with you next week to see if there is any other information I can provide, or to arrange for an interview.”

By taking the initiative to request follow-up, you demonstrate your eagerness and initiative. You also give the employer a clear sense of what to expect and when, which can help move the process forward smoothly.

Reviewing and Editing the Letter

One of the most critical steps in writing an effective solicited application letter is reviewing and editing it to ensure that it is error-free and meets the employer’s requirements. Below are two essential steps you need to take to improve your letter’s chances of making a positive impact on the employer.

Checking for Spelling and Grammar Errors

One of the biggest reasons why solicited application letters fail to make the cut is because of spelling and grammar mistakes. Nothing can turn off a potential employer faster than a sloppily written letter filled with errors. Therefore, check your letter several times to ensure that it is free of spelling and grammar mistakes.

Here are some tips to help you check for spelling and grammar errors:

• Read through your letter slowly and use a spell-checker to identify any misspelled words.
• Ensure that your punctuations, such as commas and periods, are appropriately placed.
• Read your letter again backwards to check for spelling errors.
• Print out your letter and read it aloud to yourself.

By taking the time to check for spelling and grammar errors, you demonstrate your attention to detail, which is a quality that employers value.

Getting Feedback from a Trusted Source

After you have checked your letter for language errors, you should seek feedback from a trusted source. A trusted source can help you identify and correct any weaknesses in your letter and improve its organization and wording.

Here are some guidelines to find a trusted source for feedback:

• Choose someone who is knowledgeable in the field or industry you are applying for.
• Look for someone who has experience with solicited application letters and can give constructive feedback.
• Avoid individuals who may sugarcoat their feedback or may be too harsh.

When presenting your letter for feedback, it is best to be open-minded and receptive to criticism. Remember, the goal is to improve your letter’s chances of standing out to the employer. Therefore, take note of any feedback you receive and revise your letter accordingly.

Reviewing and editing your solicited application letter is a crucial part of the job application process. By checking for spelling and grammar errors and seeking feedback from a trusted source, you can increase the likelihood of your letter making a positive impact on a potential employer.

Example of Solicited Application Letter

When it comes to applying for a specific position, it’s important to make sure that your application letter is tailored to the job and company you’re applying to. To help you get an idea of what this kind of letter looks like in action, let’s take a look at a sample solicited application letter for a marketing position:

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How to Write a Job Application Letter (With Examples)

What Is a Job Application Letter?

Tips for writing a job application letter, how to get started.

• Writing Guidelines
• What to Include in Each Section

Simple Formatting Using a Template

Tips for writing an effective letter, sample job application letter, sending an email application, review more letter examples.

Do you need to write a letter to apply for a job? Most of the time, the answer is yes. Even when employers don’t require a job application letter , writing one will help you highlight your skills and achievements and get the hiring manager’s attention. The only time not to send one is when the job listing says not to do so. It can help, and it definitely won't hurt to include an application letter with your resume.

A job application letter, also known as a cover letter , should be sent or uploaded with your resume when applying for jobs. While your resume offers a history of your work experience and an outline of your skills and accomplishments, the job application letter you send to an employer explains why you are qualified for the position and should be selected for an interview.

Writing this letter can seem like a challenging task. However, if you take it one step at a time, you'll soon be an expert at writing application letters to send with your resume.

Melissa Ling / The Balance

Before you begin writing your job application letter, do some groundwork. Consider what information you want to include, and keep in mind that space is limited.

Remember, this letter is making a case for your candidacy for the position. But you should do more than just regurgitate your resume. Instead, highlight your most relevant skills, experiences, and abilities.

Analyze the Job Posting

To include the most convincing, relevant details in your letter, you'll need to know what the employer wants.

The biggest clues are within the job advertisement, so spend some time decoding the job listing . Next, match your qualifications with the employer's wants and needs.

Include Your Most Relevant Qualifications

Make a list of your relevant experience and skills. For instance, if the job ad calls for a strong leader, think of examples of when you've successfully led a team. Once you've jotted down some notes and have a sense of what you want to highlight in your letter, you're ready to start writing.

Writing Guidelines for Job Application Letters

Writing a job application letter is very different from a quick email to a friend or a thank-you note to a relative. Hiring managers and potential interviewers have certain expectations when it comes to the letter's presentation and appearance, from length (no more than a page) and font size to style and letter spacing . Keep these general guidelines in mind, but always stick to any explicit instructions in the job listing or application portal.

Length: A letter of application should be no more than one page long. Three to four paragraphs are typical.

Format and Page Margins: A letter of application should be single-spaced with a space between each paragraph. Use 1-inch margins and align your text to the left, which is the standard alignment for most documents.

Font: Use a traditional font such as Times New Roman, Arial, or Calibri. The font size should be between 10 and 12 points.

What to Include in Each Section of the Letter

There are also set rules for the sections included in the letter, from salutation to sign-off, and how the letter is organized. Here's a quick overview of the main sections included in a job application letter:

Heading: A job application letter should begin with both your and the employer's contact information (name, address, phone number, email), followed by the date. If this is an email rather than an actual letter, include your contact information at the end of the letter, after your signature.

•   Header Examples

Salutation: This is your polite greeting. The most common salutation is "Dear Mr./Ms." followed by the person's last name. Find out more about appropriate cover letter salutations , including what to do if you don't know the person's name or are unsure of a contact's gender.

Body of the letter: Think of this section as having three distinct parts.

In the first paragraph , you'll want to mention the job you are applying for and where you saw the job listing.

The next paragraph(s) are the most important part of your letter. Remember how you gathered information about what the employer was seeking, and how you could meet their needs? This is where you'll share those relevant details on your experience and accomplishments.

The third and last part of the body of the letter will be your thank you to the employer; you can also offer follow-up information.

Complimentary Close: Sign off your email or letter with a polite close, such as "Best," or "Sincerely," followed by your name.

• Closing Examples

Signature: When you're sending or uploading a printed letter, end with your handwritten signature, followed by your typed name. If this is an email, simply include your typed name, followed by your contact information.

• Signature Examples

Overwhelmed by all these formatting and organization requirements? One way to make the process of writing a job application easier is to use a template to create your own personalized letters. Having a template can help save you time if you are sending a lot of application letters.

Be sure that each letter you send is personalized to the company and position; do not send the same letter to different companies.

• Always write one. Unless a job posting explicitly says not to send a letter of application or cover letter, you should always send one. Even if the company does not request a letter of application, it never hurts to include one. If they do ask you to send a letter, make sure to follow the directions exactly (for example, they might ask you to send the letter as an email attachment or type it directly into their online application system).
• Use business letter format. Use a formal business letter format when writing your letter. Include your contact information at the top, the date, and the employer’s contact information. Be sure to provide a salutation at the beginning and your signature at the end.
• Sell yourself. Throughout the letter, focus on how you would benefit the company. Provide specific examples of times when you demonstrated skills or abilities that would be useful for the job, especially those listed in the job posting or description. If possible, include examples of times when you added value to a company.

Numerical values offer concrete evidence of your skills and accomplishments.

• Use keywords. Reread the job listing, taking note of any keywords (such as skills or abilities that are emphasized in the listing). Try to include some of those words in your cover letter. This will help the employer see that you are a strong fit for the job.
• Keep it brief. Keep your letter under a page long, with no more than about four paragraphs. An employer is more likely to read a concise letter.
• Proofread and edit. Employers are likely to overlook an application with a lot of errors. Read through your cover letter, and if possible, ask a friend or career counselor to review the letter. Proofread for any grammar or spelling errors.

This is a job application letter sample.  Download the letter template (compatible with Google Docs or Word Online) or read the example below.

Sample Job Application Letter (Text Version)

Elizabeth Johnson 12 Jones Street Portland, Maine 04101 555-555-5555 elizabethjohnson@emailaddress.com

August 11, 2024

Mark Smith Human Resources Manager Veggies to Go 238 Main Street Portland, Maine 04101

Dear Mr. Smith,

I was so excited when my former coworker, Jay Lopez, told me about your opening for an administrative assistant in your Portland offices. A long-time Veggies to Go customer and an experienced admin, I would love to help the company achieve its mission of making healthy produce as available as takeout.

I’ve worked for small companies for my entire career, and I relish the opportunity to wear many hats and work with the team to succeed. In my latest role as an administrative assistant at Beauty Corp, I saved my employer thousands of dollars in temp workers by implementing a self-scheduling system for the customer service reps that cut down on canceled shifts. I also learned web design and timesheet coding, and I perfected my Excel skills. 

I’ve attached my resume for your consideration and hope to speak with you soon about your needs for the role.

Best Regards,

Elizabeth Johnson (signature hard copy letter)

Elizabeth Johnson

When you are sending your letter via email include the reason you are writing in the subject line of your message:

Subject Line Example

Subject: Elizabeth Johnson – Administrative Assistant Position

List your contact information in your signature, rather than in the body of the letter:

Email Signature Example

Elizabeth Johnson 555-555-5555 email@emailaddress.com

Review more examples of professionally written cover letters for a variety of circumstances, occupations, and job types.

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Best Solution To Write A Job Application Letter With A Reference Person

There are circumstances wherein you’re trying to get into a position in a firm and also have recommendations from the firm’s employees. You might think if it’ll benefit you at all. But studies have shown that referrals are game-changers when it comes to employment. While hiring, employees are looking for those candidates that are reliable, best suited for the job, and good enough to carry it onward. 

Now if you have a referral from someone in the office, your interviewer doesn’t have to worry a lot about you and can just ask about you the mutual contact. That can reduce his work and if you get a good word put in, it can also increase the chances of you getting a job. In this manner, it’s great to be referred to by someone when it comes to jobs. But how can you put them in your job application letter? What is the correct way to specify that there is mutual contact that can vouch for you and can second the fact that you’re a good fit for the job? You don’t want to put the referral in the wrong way or it can also turn against you. So don’t fret, we have you covered with the best solution to write a job application letter with a reference person. 

Top Solutions To Write Job Application Letter With A Reference Person

Here are some tips to write a job application letter with a reference person

Mention Who You’re Referred by

The first and foremost thing that you can do to write a job application letter with a reference person is to mention who you’re referred by. It can set a great start for your letter and can explain a lot of things such as how you came to know about the vacancy. This also ensures that you set the letter straight off your referral which can remove any awkwardness and give you a nice starting point to begin from. 

You can write this something like “my previous colleague and your executive Sarah Martin introduced me to a sales representative vacancy and suggested that I direct you for it”. Note that you have to keep a formal tone and don’t give too many details about the encounter where you came to know about the job. It’s not okay to mention “I stumped into Sarah Martin at a local grocery shop where she told me about a vacancy in her company”. This is not the right way. Be precise and formal. Just mention the name of the person to whom you’re referred followed by how you know them. 

Why are They Referring to You?

Now, the biggest question that arises is why is that person from the company referring to you at all. Is that only because of a personal connection or is it more professional? While it will be very easy to judge if you’re referred only based on personal connections by the interviewer, you should always look for the reason for why you’re referred by the person before writing the letter. This is because, in the job application letter with the reference person, you are also putting their choice in the question. 

So, after that you’ve introduced yourself and explained your connection with the mutual contact, it will be suitable to write something that will explain why you’re referred to by them. This can be any line that suitably explains your qualifications and skills that can help at the job. You can write anything that goes along the lines of “Sarah thought that considering my 5 years background in sales with 2 awards due to my profits, I would be a great addition to your firm (firm name)”. Additionally, you can also write something like “As she can confirm, I tend to work in a unique manner which can fit amazingly with the working pattern of your company and can thereby profit you”. With this line, you have to explain how you’re capable enough for the job considering your referral. 

Check Examples

If you’re confused about how to go about this letter, it is always best practice to search online for “job application letter with reference person” and look for the available templates to check the tone and language that you should use for the letter. It can help you greatly by telling you the structure in which the contents of the letter flow and will warn you of any mistakes that you were going to make. Ensure that you don’t copy-paste any of the templates and just change the details. Any interviewer will be able to see that and will completely disregard your referral. You will ruin all the chances that you had so completely avoided. 

Explain Accomplishments

One of the best ways to back the person to whom you’re referred by, the best way would be to explain your accomplishments and skills. Explain why you’re a good candidate for the job. This is a very important process for any job application letter with a reference person because it will enable the interviewer to see what merits you have as a candidate and what sets you apart from others. This can further be very helpful for him to evaluate you and thereby it will ensure that you can profit from the referral to the maximum. With your accomplishments, the interviewer will come to realize that you’re a deserving candidate and the fact that you’re also referred by someone from the firm will only improve your chances further. 

There are many ways in which a referral could help you. It can make you stand apart from other candidates and can also make you a preferred candidate for the interviewer even before you interview. You just have to ensure that you make the most out of your chance by creating the best job application letter with a reference person. Before sending that letter off, it is recommended to once ask the person you’re referred by if he’s okay with his name being in the letter. Further, you can also get it reviewed by him to send in the best letter that you can. In this manner, your chances of getting a job at that firm will be significantly improved.

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• Job Application Documents

How to Write a Job Application Letter (with Examples)

Last Updated: March 21, 2024 Fact Checked

Sample Letters

Introduction, body paragraphs, closing your letter, expert q&a.

This article was written by Shannon O'Brien, MA, EdM and by wikiHow staff writer, Aly Rusciano . Shannon O'Brien is the Founder and Principal Advisor of Whole U. (a career and life strategy consultancy based in Boston, MA). Through advising, workshops and e-learning Whole U. empowers people to pursue their life's work and live a balanced, purposeful life. Shannon has been ranked as the #1 Career Coach and #1 Life Coach in Boston, MA by Yelp reviewers. She has been featured on Boston.com, Boldfacers, and the UR Business Network. She received a Master's of Technology, Innovation, & Education from Harvard University. There are 7 references cited in this article, which can be found at the bottom of the page. This article has been fact-checked, ensuring the accuracy of any cited facts and confirming the authority of its sources. This article has been viewed 10,651,338 times.

So, you’ve found your dream job and want to make sure you nail the job application process. You double- and triple-check the criteria—they’re asking for a cover letter. What does that mean, and how do you write it? A cover letter or letter of application is a single page that sums up why you want and deserve the job. Think of it as an extension of your resume; a sales pitch for why you’re the perfect candidate. We’ve put together a step-by-step guide full of examples and tips on how to write a letter of application for a job. With our help and a little finesse, you may soon be calling that dream job your own.

Things You Should Know

• Format your application letter single-spaced and in Times New Roman, Arial, or Calibri font that’s 10- to 12-point in size.
• Open your letter with an engaging and confident first paragraph that briefly includes your qualifications, where you found the job, and your overall interest in the position.
• Show your personality in the body paragraphs by describing the passions that relate to the position in 1 or 2 sentences.
• Use keywords (like leadership, communication, and detail-oriented) from the job description throughout your letter to show that you’ve done your research.

• First and last name
• Phone number
• Email address
• Personal website and/or portfolio link (if you have one)

• If you don’t know the hiring manager's name, search the company’s website or refer to the name of the individual who originally posted the job opening.
• If you’re in doubt about who to address your letter to, use “[Department] Hiring Manager.”

• If you don’t have the employer or hiring manager’s name, use a general but professional opening, “To Whom It May Concern” or “Dear [Department] Hiring Manager.”

• Be short and specific in this opening paragraph—save those details for later.
• Think of your first paragraph as a sales pitch. What can you say that’ll grab their attention immediately? Is there something you have that other candidates don’t that make you more qualified for the position?
• Show the employer that you’re familiar with the company and job application by noting keywords and characteristics valued by the company.
• For example: “I write to apply for the Office Manager position at Acme Investments, Inc. I am an excellent fit for this position, as demonstrated by my extensive background in management and proven success as a corporate administrator.”

• Companies appreciate when job candidates include this information because it lets them know where people are searching for jobs.
• Only include a company contact or friend’s name if you have their permission. This way, they’ll be ready to answer any questions about you and your character later.
• You may write something like: “John Smith recommended that I get in touch with you about the general manager position at EnviroRent,” or “I came across the available position on LinkedIn and believe I am a strong candidate.”

• For instance, if the company needs someone who can lead a team and handle multiple projects at once, note what team projects you’ve led in previous positions and how you improved overall productivity.
• If you have numerical data or stats to back up your accomplishments, include them! This is your time to brag about your achievements and show how you’ve excelled in the workplace.

• Scan the job application for keywords like leadership, communication, management, and detail-oriented. Then, highlight in your letter how you have these characteristics or skills.
• Avoid embellishing any of your qualifications. Remember, an employer can always double-check the facts.
• If you’re not sure what to write, refer to your resume or CV. What have you done that matches the job description best, and how can you elaborate on it?
• For example: “In my previous role, I successfully supported an office of 100 personnel and honed my management and interpersonal skills through customer service and clerical responsibilities.”

• For instance, you could express how the company has impacted you personally and why that’s driven you to apply for the position.
• Although you want to provide details, keep it short. Stick to a 1 to 2-sentence description rather than a full-length story. Your letter should stay under 3 paragraphs.
• Here’s an example: “My passion for teaching began the summer of my sophomore year of high school when I was a camp counselor. I was given the opportunity to teach a class focusing on local plant life, and the campers’ enthusiasm cultivated my love for teaching and conservation.

• For instance, you could write, “I am excited about the possibility of working for you and your company. I would be more than happy to discuss my qualifications and Acme’s future direction in person or via video conference.”
• Keep your call to action brief and open, or provide specific dates you’d be available to meet with the employer.

• For instance, sign off with, “Thank you for your time and consideration. I look forward to hearing from you,” or “Thank you for considering me for this position. If you have any further questions or require additional documentation, please don’t hesitate to contact me.” [12] X Research source

• If you’re sending your letter via email, import your signature into the document as an image or .png file.

• Always proofread and ask someone else to read over your application letter before you send it. This way, you can make sure it’s absolutely perfect and error-free. [14] X Trustworthy Source Purdue Online Writing Lab Trusted resource for writing and citation guidelines Go to source Thanks Helpful 0 Not Helpful 0
• Keep the overall tone of the company or employer in mind while writing your letter. For instance, if you’re applying to be a journalist for a prestigious news website, match their word choice and writing style. Thanks Helpful 0 Not Helpful 0
• Be sure to customize your application letter for every job you apply to, even if they have the same qualifications. Thanks Helpful 0 Not Helpful 0

You Might Also Like

• ↑ https://www.ferrum.edu/downloads/careers/cover-letters.pdf
• ↑ https://owl.purdue.edu/owl/job_search_writing/job_search_letters/cover_letters_workshop/formatting_and_organization.html
• ↑ https://icc.ucdavis.edu/materials/cover-letters
• ↑ https://hbr.org/2016/05/learn-to-love-networking
• ↑ https://hbr.org/2014/02/how-to-write-a-cover-letter
• ↑ https://www.astate.edu/dotAsset/54eb42cc-33a3-4237-a46e-3f4aaac79389.pdf
• ↑ https://career.gatech.edu/writing-effective-cover-letter

About This Article

The best way to start an application letter is to mention where you found the job opportunity and how your strengths can benefit the employer. Devote time in the body paragraphs to tell the employer more about your experience and qualifications. Explain why you’re the best candidate and finish by inviting the hiring manager to contact you. For suggestions on how to prepare your letter, and examples of what to write, read on! Did this summary help you? Yes No

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10 Advertising Cover Letter Examples

Advertisers are experts at creating captivating campaigns that grab attention and persuade audiences to take action. Similarly, your cover letter is your personal marketing campaign, designed to capture the interest of recruiters and convince them of your value. In this guide, we'll explore top-notch Advertising cover letter examples, demonstrating how to effectively sell your skills, experience, and passion for the industry.

Cover Letter Examples

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The best way to start an Advertising cover letter is by grabbing the reader's attention immediately. This could be achieved by mentioning a recent successful campaign you've worked on, or by expressing your passion for a campaign the company has recently run. It's also important to address the hiring manager by name if possible, showing that you've done your research. For example, "When I saw your recent campaign for X, I was impressed by its creativity and impact. As a seasoned advertising professional with a track record of creating successful campaigns, I am excited about the opportunity to contribute to your team." This approach shows your enthusiasm for the company and your relevant experience.

Advertises should end a cover letter by summarizing their interest in the position and the unique skills they bring. They should reiterate their enthusiasm for the role and the company, and express a desire for further discussion. A strong closing might be: "I am excited about the opportunity to bring my unique blend of creativity, strategic thinking, and data-driven decision making to your team. I look forward to the possibility of discussing my application further." Always remember to thank the reader for their time and consideration. The closing should be professional and positive, leaving a lasting impression. Sign off with a professional closing like "Sincerely" or "Best regards," followed by your name.

An Advertising cover letter should ideally be about one page in length. This is because hiring managers often have to go through numerous applications, so a concise, one-page cover letter ensures your key points are easily digestible. It's enough space to highlight your most relevant experiences, skills, and achievements without overwhelming the reader. Remember, in advertising, being able to communicate effectively and succinctly is a valued skill, and your cover letter is a great place to demonstrate this.

Writing a cover letter with no experience in Advertising can seem daunting, but it's important to remember that everyone starts somewhere. Here's a step-by-step guide on how you can approach this: 1. Contact Information: Start with your contact information - your name, address, phone number, and email address. 2. Salutation: Address the letter to the hiring manager. If you don't know their name, use a general salutation like "Dear Hiring Manager". 3. Introduction: Begin your letter by introducing yourself and stating the position you're applying for. Mention where you found the job posting. 4. Highlight Relevant Skills: Even if you don't have direct experience in advertising, you likely have skills that are relevant. Maybe you've taken courses in marketing or communication, or perhaps you've worked in sales or customer service. Highlight these skills and explain how they're relevant to the role you're applying for. 5. Show Enthusiasm: Show your passion for the industry. Maybe you've always been fascinated by how advertisements can influence consumer behavior, or perhaps you love the creativity involved in developing ad campaigns. Whatever it is, make sure to convey your enthusiasm. 6. Provide Examples: Use specific examples to demonstrate your skills. For instance, if you've taken a marketing course, talk about a project where you had to develop a marketing plan. If you've worked in sales, discuss how you used persuasion techniques to convince customers to buy a product. 7. Show You've Done Your Homework: Research the company and mention something you admire about it in your cover letter. This shows you're serious about wanting to work there. 8. Closing: In your closing paragraph, thank the hiring manager for considering your application. Express your interest in the opportunity to discuss your qualifications further. 9. Signature: End with a professional closing like "Sincerely" or "Best regards," followed by your name. Remember, the goal of a cover letter is to show the hiring manager why you'd be a good fit for the role, even if you don't have direct experience. Highlight your transferable skills, show your passion for the industry, and demonstrate that you've taken the time to research the company.

Related Cover Letters for Advertisings

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Brand Strategist Cover Letter

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Advertising Manager Cover Letter

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How to Write an Effective Application Letter (Examples)

By Status.net Editorial Team on November 15, 2023 — 9 minutes to read

Your application letter should be a clear reflection of you, your skills, and your aspirations. It’s essential to tailor it to the specific job you’re applying for and showcase how you meet the requirements. Stay with us as we walk you through the tips, tricks, and best practices to make your letter shine. By the end, you’ll have the knowledge and confidence to navigate the job application process with ease.

Step 1. Introduction: Expressing Interest

The opening line.

Your opening line should grab the reader’s attention, briefly introduce yourself, and express your interest in the position. This is your opportunity to make an excellent first impression, so keep it clear and concise. For example, you could start with:

“As an experienced marketing professional, I was thrilled to see the opportunity for a Marketing Manager position at X Company.”

Revealing the Source of Information

Next, it’s important to mention where you found out about the job opening. This helps recruiters understand where their outreach efforts are effective and demonstrates that you’ve done your homework. Reference the specific platform, such as a job board or company website, or mention the person who referred you to the position. Here are a couple of examples:

“I came across this position on LinkedIn and believe my skillset aligns perfectly with the job requirements.” or

“Jane Brown, the Sales Director at your company, suggested I apply for this role, as she believes my experience in customer service is a great fit for the team.”

Step 2. Body: Detailing Qualifications

Pitching your skills.

When writing an application letter, it’s essential to showcase your skills. Start by listing the most relevant ones based on the job description. Be specific and mention how you’ve used these skills in previous projects or work settings. For example:

“As a project manager, I have successfully managed teams of up to 20 members, ensuring timely delivery of projects while maintaining a high level of quality.”

Use bullet points or bold text to make your skills stand out. This way, the reader gets a clear picture of your capabilities.

Referencing Your Experience

After listing your skills, provide details about your work experience. Start with the most recent position and include the name of the company, your job title, and the duration of employment. Focus on the responsibilities that match the job opening. For instance:

“During my tenure at X Corp as a marketing executive, I was responsible for coordinating marketing campaigns, managing social media channels, and conducting market research.”

Don’t forget to mention any relevant internships or volunteer work. This information will help paint a complete picture of your expertise.

Demonstrating Your Achievement

Lastly, highlight your accomplishments and show the potential employer why you’re a perfect fit. Use concrete examples and mention any quantifiable results you’ve achieved. For example:

“At ABC Inc., I initiated a cost-reduction program that saved the company $50,000 within six months.”

You can also mention any awards or recognitions you’ve received for outstanding work. This demonstrates that your contributions have been valued and recognized by others.

Step 3. Concluding Your Letter

Seeking further communication.

By the time you reach the end of your application letter, it’s important to express your desire for further communication with the potential employer. This shows that you’re genuinely interested in the opportunity and eager to continue the conversation. Example:

“I am excited about the prospect of contributing to your company’s goals, and I would appreciate the opportunity to discuss this further with you. Please don’t hesitate to contact me at your earliest convenience. Thank you for considering my application.”

Final Goodbye

After expressing your eagerness, close your letter with a polite and professional farewell, addressing the recipient by name where possible. This is not only courteous, but it also leaves a positive and lasting impression. Example:

“ I look forward to your response and the possibility of working together. Once again, thank you for your time and consideration. Sincerely, [Your Name]”

Proper Letter Ending

The complimentary close.

Start by choosing an appropriate complimentary close for your application letter. This part signifies the end of the main content and should leave a professional impression. Some common examples are “Sincerely,” “Best regards,” or “Yours faithfully.” Keep in mind that it’s best to stay formal, so avoid using casual phrases like “Cheers” or “Take care.”

After the complimentary close, press enter twice to leave a space for your signature. This space provides room for your actual signature if you’re submitting a physical letter. If you’re submitting electronically, this space can act as a visual cue that your letter has reached its end.

While signing an application letter, be sure to include your typed full name. Don’t forget to include your relevant contact information, such as your email address or phone number. This will make it easy for the recipient to get in touch with you if they have any questions or require additional information.

Here’s an example of a proper letter ending for your application letter:

[Space for physical signature, if applicable] Your Full Name [email protected] +1-234-567-8901

Post-Writing: Proofreading and Correcting

After writing your application letter, it’s essential to proofread and correct any errors or inconsistencies. This process will help ensure that you submit a polished and professional document that impresses potential employers.

Correcting Grammar and Punctuation

First, focus on your grammar and punctuation. A well-written letter that follows proper grammar rules is more likely to capture the reader’s attention and convey your message effectively.

• As you’re reading through your letter, keep an eye out for missing or misplaced commas, semicolons, and other punctuation marks.
• Check for sentence fragments or run-on sentences that make your message unclear.
• Look for subject-verb agreement issues, as well as any awkward phrasing or wordiness.
• Ensure consistency in tense and voice throughout the letter.

Checking for Spelling Mistakes

Your next step should be checking for spelling mistakes. Misspelled words can distract the reader and make your application appear less polished.

• Run your text through a spellchecker; most word processing programs have this feature built-in.
• Take the time to read through your letter carefully, word-by-word, to catch any errors the spellchecker may have missed.
• Double-check the spelling of names, addresses, and other specific information to make sure they’re correct.

Examples of Successful Application Letters

When writing an application letter, it’s essential to tailor it to the specific job posting . Check out these examples to help you create a winning letter for different scenarios.

Dear [Hiring Manager],

I’m excited to apply for the Sales Representative position at [Company Name]. With my proven sales record and strong interpersonal skills, I believe I would be a valuable asset to your team.

In my previous role at [Previous Company], I consistently exceeded sales targets and established strong relationships with clients. I’m confident that my experience and passion for sales will contribute to the ongoing success of [Company Name].

Thank you for considering my application. I look forward to discussing my qualifications and how I can contribute to the growth of [Company Name].

Sincerely, [Your Name]

As a creative and skilled Graphic Designer, I am thrilled to apply for the position at [Company Name]. My expertise in Adobe Creative Suite and concept development aligns with the requirements laid out in the job posting.

In my previous role at [Previous Company], I created visually appealing and engaging content for various marketing campaigns. My designs helped increase brand recognition and lead to a 20% increase in social media engagement. I am eager to use my talents and contribute to the visual identity of [Company Name].

I look forward to the opportunity to discuss my skills and portfolio with you. Thank you for considering my application.

Best regards, [Your Name]

As an experienced Office Manager with a strong background in time management and organization, I am eager to apply for the position at [Company Name]. Your commitment to efficiency and supporting your employees is in line with my work values.

During my tenure at [Previous Company], I streamlined scheduling and developed procedures that led to a 30% reduction in office expenses. My proactive approach to problem-solving and ability to create a productive work environment contribute to my effectiveness as an Office Manager.

I am enthusiastic about the opportunity to contribute to the success of [Company Name] by enhancing office operations. Thank you for considering my application.

Frequently Asked Questions

What are the key components of an application letter.

An application letter should include the following key components:

• Contact information: Start by writing your name, address, phone number, and email address.
• Salutation: Address the recipient professionally, using their name when possible.
• Opening paragraph: Introduce yourself and state the position you’re applying for.
• Body paragraphs: Highlight your relevant skills, experience, and accomplishments.
• Closing paragraph: Reiterate your interest in the position, provide your contact information, and thank the reader for considering your application.
• Sign-off: Use a polite closing, such as “Sincerely” or “Best regards,” followed by your name.

Can you provide a step-by-step guide on writing a cover letter?

• Review the job posting and research the company to understand their needs and values.
• Write your contact information at the top of the letter.
• Use a professional salutation and address the recipient by name, if possible.
• Craft an engaging opening paragraph that states the position you’re applying for and how you learned about it.
• Write body paragraphs that showcase your relevant skills, experience, and accomplishments, drawing connections to the requirements mentioned in the job posting.
• In the closing paragraph, restate your interest in the position and thank the reader for their time.
• Sign off with a polite closing and your name, followed by your phone number and email address.

What are some tips for writing an effective application letter?

To write an effective application letter:

• Tailor the content: Focus on the skills and experiences that are most relevant to the specific job posting.
• Use strong action words: Highlight your achievements using action verbs, such as “managed,” “achieved,” or “developed.”
• Proofread for errors: Thoroughly check your letter for spelling and grammatical errors before sending.
• Maintain a professional tone: Write your letter with a confident and respectful tone, avoiding slang or overly casual language.

How do you customize your cover letter for different job positions?

Make sure to modify your application letter to suit the specific job and company you’re applying to. Analyze the job posting to understand the key requirements and skills the employer is looking for. Emphasize how your experiences and abilities address these needs. Research the company to understand their values and culture, and incorporate that knowledge into your letter to show you would be a good fit for their organization.

What are some common cover letter mistakes to avoid?

Some common mistakes to avoid in cover letters include:

• Not customizing your letter for each job or company
• Focusing too much on yourself and not on the needs of the employer
• Including too much information or making the letter too long
• Repeating your resume verbatim without providing more context
• Failing to proofread for spelling and grammatical errors

How can I make my application letter stand out from the competition?

To make your application letter stand out:

• Use a compelling opening to grab the reader’s attention.
• Show enthusiasm for the position and the company.
• Make sure your letter is well-organized and visually appealing, with a professional font and layout.
• Tailor your letter to the specific job and company, focusing on the most relevant skills and experiences.
• Offer examples of your achievements to demonstrate your ability to succeed in the role.
• Proofread your letter to ensure it is error-free and polished.
• How to Write a Letter of Employment (Templates, Examples)
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• How to Write an Effective Performance Review (Essential Steps)

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Application Letter for Job with Reference to Advertisement

Posted By Compulsory English Leave a Comment

APPLICATION LETTER _Advertisemnet

Techonkreet Techno

Sg Ramal Luar

43000 Kajang

Selangor, Malaysia

Date – 23rd December, 2018

Sub: Application Letter for job with reference to Advertisement in The Daily on 29th Dec, 2018

Dear Sir / Madam,

This is in response to your advertisement ‘Required Graduates in any discipline for office work’ published in The Daily on 29th December, 2012. I am interested in applying for the aforesaid post.

I am Ms. Irine Abang, 36 years old, graduate from Selangor University’s batch of year 2000. I have worked for High Climb and Make Stock as an Adviser. I am willing to attend interview & test and relocate as per your convenience. I hope, I will be considered for your company’s employment process. I have enclosed a copy of my C.V. and experience certificates for your review.

Your assistance and attention to this application is highly appreciated.

Thank you for your time.

Looking forward to hear from you.

Respectfully Yours

Irine Abang

Sarawak, Malaysia

Phone: 802 – 418705

[email protected]

Encl: Photocopy of C.V.

Experience Certificates

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How to List References in a Cover Letter

Last Updated: April 2, 2019

This article was co-authored by Melody Godfred, JD . Melody Godfred is a Career Coach, Entrepreneur, and Founder of Write In Color, a full-service resume and career development company that specializes in developing compelling personal narratives and brands. With over ten years of experience, Melody has worked with clients at entertainment and media companies including Apple, Disney, Fox, Netflix, Riot Games, Viacom, and Warner Bros, among others. The Muse invited Melody and Write In Color to serve as one of its 30 trusted career counselors (out of 3,000) to provide one-on-one coaching and resume services to the platform's more than four million active users. Melody earned a JD from Loyola Marymount University and BS from the University of Southern California. This article has been viewed 29,886 times.

References are people you can rely on to provide positive recommendations for you when applying for jobs or higher education opportunities. The people you list as references should know your abilities, previous experience and skills you possess, and be willing to recommend you as a viable candidate for the positions for which you apply. Follow these steps to learn how to properly create a reference list.

Selecting Your References

• You could also select colleagues, peers or co-workers who can provide insight to your work ethic, skill levels and abilities. People who can provide examples detailing your positive qualities because of your work relationships are effective references.
• Consider people with whom you've volunteered or worked with through a non-profit organization. Despite not being paid for your work, you may have shown leadership or other skills that can be valuable as a candidate for a job or higher education. People with whom you've volunteered may be able to share details about your skills.

• When asking someone to be your reference, make sure that they plan on giving you a positive recommendation. You should try to select people you know will give you an excellent recommendation.

• You should not mention references unless you are asked to do so by the company or program you are applying to.

Writing Your Reference List

• If you would like to make a clear break between your header and the content of your page, add a line below your contact information.

• Write down your first reference. His or her name should be in bold. Underneath his/her name, write his/her relationship to you (ie. Professor, or Boss) and the company or program that you both worked for.
• Write down the company’s address if your reference still works at that company. If he/she does not, do not list any address.
• List your reference’s contact information a line below your relationship to your reference. This should be the contact information they provided you--either a telephone number, email, or both.

Expert Q&A

• Only list references that you know for a fact will give you a positive review. If there is any doubt, try to come up with a different reference. Thanks Helpful 0 Not Helpful 0
• Double and triple check the spelling of your references' names. Misspelling their names could show a lack of attention to detail or disrespect on your part. Thanks Helpful 0 Not Helpful 0

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• ↑ http://uhr.rutgers.edu/worklife-balance/life-events/layoff-information/preparing-resume-and-cover-letter

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09+ Application letter for the job advertisement Samples Free

Looking for Application letter for the job advertisement? When applying for a job, it is important to submit a resume and cover letter that reflect your skills and experience. However, many job postings also require an application letter. An application letter is different from a cover letter in that it provides more information about why you are interested in the position and how you meet the qualifications listed in the job advertisement. It can be difficult to know what to include in an application letter, so here are some tips on how to write one.

What is Application letter for the job advertisement?

Applying for a job can be a daunting task, but one of the most important parts of the process is the application letter. This is your opportunity to introduce yourself to the employer and explain why you are the best candidate for the position. The letter should be professional and well-written, and it should include a specific mention of the job you are applying for. In addition, the letter should highlight your key qualifications and explain why you would be a good fit for the company. Application letters can seem like a lot of work, but if done correctly, they can give you a big advantage in the hiring process.

Application letter for the job advertisement format

The email format for sending a letter is simple and includes just five essential parts:

• Subject line ( How to write a good email subject line That Opened 10x )
• Opening line and body

Application letter for the job advertisement

Letter Sample 2:

Related: 17 Job Inquiry Letters & Email Templates

Sample job application letter in response to an advertisement

Letter Sample 4:

Letter Sample 6:

Related: 09+ Meeting Request Email Sample To Manager Free Templates

Job application letter with reference to newspaper ad

Letter Sample 8:

Application letter for the job advertisement subject lines

Application letters are written for various reasons like: job application, admission application, scholarship application, internship application etc. The template is a general format that can be used for all types of application letters. This Application Letter Template lists the information you need to include in the letter you submit with your resume when applying for a job. The Components of a Application Letter template:

Heading: The heading should include your name, address, telephone number, email address, and the date.

Opening paragraph: The opening paragraph should state the purpose of the letter.

Middle paragraph(s) : The body of the letter should explain why you are qualified for the position you are applying for. Be sure to mention specific examples from your experience that show off your skills and abilities.

Closing paragraph: The closing paragraph should thank the employer for taking the time to review your qualifications and let them know how they can get in touch with you if they decide to offer you an interview.

Application Letter Template Date Name of Contact Person (if available) Title Organization Address City, State Zip Code Dear Mr./Ms. Last Name: First

10 Common mistakes When Writing application letter

When you’re applying for a job, it’s important to include a well-written application letter. This letter gives you a chance to introduce yourself and explain why you’re the best candidate for the position. To ensure that your application letter makes a good impression, make sure to avoid these 10 common mistakes:

• Addressing the wrong company or contact person.
• Using a generic salutation such as “To whom it may concern.”
• Starting with “I’m writing to apply for the [position] job.”
• Failing to mention why you’re the best candidate for the job.
• Including such irrelevant information as your GPA or SAT scores.
• Listing your work history in chronological order instead of highlighting your most relevant experience first.
• Making grammar and spelling errors.
• Using jargon or abbreviations without explanation
• Failing to modified your resume or application letter to fit the specific job requirements
• Sending an email with a unprofessional sounding email address such as [email protected]

How to Start Application letter for the job advertisement

A job application letter, also known as a cover letter, is a document sent with your resume to provide additional information on your skills and experience. The letter is often customized to the position you are applying for and should include an introduction, body, and conclusion.

The introduction of your application letter should briefly state who you are and why you are writing. The body of the letter should detail your qualifications and experience that make you a good fit for the role. It is important to customize each application letter to the job you are applying for, so be sure to include specific examples from your work history that illustrate your skills. The conclusion of the letter should thank the employer for their time and mention how you will follow up.

Sending a job application letter is a chance to sell yourself as the perfect candidate for the role. By following these tips, you can ensure that your letter will make a strong impression on the hiring manager.

How to END Application letter for the job advertisement

Application letters are an essential component of the job application process. A well-written application letter will ensure that your application stands out from the competition and increases your chances of being invited for an interview. Application letters should be concise and to the point.

The opening paragraph should state the position you are applying for and why you are interested in the role.

The second paragrap h should highlight your skills and experience that make you ideally suited for the position.

The third paragraph should thank the employer for their time and interest in your application.

Finally, the closing paragraph should restate your interest in the role and express your availability for an interview. By following these simple tips, you can ensure that your application letter makes a great impression on potential employers.

I hope you found this blog post helpful as you search for a new job. The application letter samples we provided give you a great starting point as you draft your own document. Remember to tailor each letter to the specific position and company you are applying to, and be sure to highlight your unique skills and experiences. Best of luck in your job search!

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About the author

Write A Letter for Application in Response to the Following Advertisement

Writing job applications in response to advertisements is an essential part of letter writing. It helps you to pass competitive exams and is also used to apply for jobs whenever you need it. The information in your CV should resemble your job application.

The following are a few examples of job applications in response to the advertisement.

1. Simple Job Application in Response to the Advertisement

To The HR Manager, ABC Power Group, Survey No. 35/1, Ashok Nagar, Bangalore, Karnataka 560025.

Sub: Application for the post of Sales Executive, Ref: Ad in Hindu Paper

Dear Sir/Madam,

This is in reference to your advertisement in Hindu Paper on 21 Aug 2023 for the position of Sales Executive in ABC Power Group . I would like to apply for the position.

My name is Suresh Koduru , I completed my B.Com Computers from Andhra University in 2023 . I am self-motivated and driven by targets. I am proficient in communication, both verbal and written.

Please take the time to refer to my attached resume. I would be grateful if you give me an opportunity to work with your esteemed organization.

You can contact me at my e-mail id [email protected] (or) at mobile number 8520XXXX25 .

Looking forward to hearing from you.

Thanking you.

Regards, Suresh Koduru.

2. Application In Response to the Advertisement Appeared in the Deccan Herald

To The Manager, ABC Tech Ltd, Adarsh Nagar, Hyderabad, Telangana 500063

Dear HR Manager,

Sub: Software Engineer Job Application, Ref: Indian Express.

With all due respect, based on your ad published in the Indian Express on 25 Aug 2023 that ABC Tech Pvt Ltd is in search of a Software Engineer . I am interested in applying for that job.

I finished my B.Sc. in Computer Science at Osmania University in 2021 . Currently, I have been working at XYZ Tech Solutions as a Software Engineer since May 2021 . I have more than 2 years of programming experience in Java, J2EE, Spring & Hibernate.

I believe I can bring significant value to your company. I am fully committed to success on every project that I take.

Please find attached my resume for your kind reference. I can make myself available for an interview at a time convenient to you.

Sandeep Desai, Mobile: 7245XXXX20 Email: [email protected]

3. Application in Response to the Advertisement Appeared in Hindu

XYZ Motors Looking for an Accounts Manager Qualification: MBA (or) M.Com Send your resume to HR Manager [email protected] Address: Athwas, Surat, Gujarat 395001.

To The HR Manager, XYZ Motors, Athwa, Surat, Gujarat 395001.

Sub: Application for Accounts Manager Role , Ref: Deccan Chronicle.

I am writing in response to your ad for an Accounts Executive published in the Deccan Chronicle on Sunday, 27 Aug 2023 . I believe my qualifications and experience fit well with the role.

I have an MBA qualification in Finance with over 4 years of experience. I am currently working with ABC Chemicals Pvt Ltd for two years. I have a strong understanding of financial statements and reports. I have a good knowledge of Tally ERP and Excel.

Please find enclosed my resume to find out more details about me. I can be reached anytime via email at [email protected] (or) my mobile no. 8525XXXX70 .

Thank you for your time and consideration.

Sincerely, Your name.

4. Write an Application in Response to the Advertisement Using Information Given in the CV Provided

To The Manager, ABC Tech Group, Narayanguda, Hyderabad, Telangana 500027.

Sub: Job Application for Digital Marketing Executive Role, Ref: Dainik Jagran.

I am sending you my resume in reference to your ad posted in Dainik Jagran on 05 Aug 2023 for the role of Digital Marketing Executive . I am confident that I have the necessary skills and abilities.

I completed my B.Com in Computers from Gayatri Jr College in 2022 . I did a six-month Digital marketing internship with ABC Tech Solutions.

I have strong knowledge in SEO, SEM, Adwords, and e-mail marketing. I can analyze web traffic metrics and suggest solutions for increasing web presence. My willingness to take responsibility for assigned duties and my curiosity will make me the right person for that position.

I look forward to talking about that job opportunity. Please feel free to contact me at my email id [email protected] or mobile no. 7456XXXX20.

Regards, Gayatri Jain.

To The Principal, School Name, Address.

Sub: Job Application for Primary School Teacher , Ref: Hindustan Times.

I am writing this in response to your ad in the Deccan Chronicle for the position of a primary school teacher. I am confident that my skills and experience are a great match for this opportunity.

I am B.Sc Mathematics graduate which I completed from Gurunanak College in 2020 . I have 3 years of experience teaching Mathematics to primary school students. Currently, I am working at Balaji Primary School, Chennai.

Please find the enclosed resume for further information about my skills and experience. I am confident that I can contribute to your organization to the fullest of my ability.

Please feel free to contact me at my email id [email protected] (or) mobile no. 9453XXXX20.

Thank you for your time, and I hope to hear from you soon.

Sincerely, Sunitha Nayyar.

Tips to Write Job Application in Response to Advertisement

1 st Paragraph: Explain how you find job details such as newspaper name, date of publication, and job title. Also, indicate your reason for applying for this position.

2 nd paragraph: State your qualifications, experience, and any other skills related to the position.

3 rd Paragraph: Mention that you have enclosed your resume for any further information about yourself.

4 th Paragraph: Finally, express your willingness to attend the interview, and provide your contact information such as email id and mobile number.

This is called a solicited application, the application that is sent in response to the company’s advertisement. If you send a job application without an ad, that is an unsolicited application.

You can send it by email or direct post. Sometimes you can also directly call the Recruiter for additional information.

Recommended:

• Job application formats for class 12 (Examples)
• Subject line examples for job application emails.

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Advertising cover letter

Cover letters are often sent to the human resources department or department that is looking for the candidates if they have a decentralized HRM structure. Cover letters emphasize the intent of the sender, that he or she is interested to work for an employer while writing for the verification purposes of the employee job. Such letters should be written with care and must be flawless, in order to make a good impression. In this context, we will guide you on how you can write an employment letter all by yourself from scratch as well as we will assist you with a sample employment letter.

How to write an application letter for a job advertised in the newspaper?

Cover letters should be formatted and drafted in such a way they emphasize the relevance of the work profile of the applicant based on the job description and the requested qualifications and experience of the applicant. Such letters should emphasize the experience gained by the person in related domains. Therefore, it's not that simple, and people with less experience best seek guidance on how to write a decent job application letter. Such applications or documents must be written with a keen eye to look like a professional. Here are attached some guidelines which you can follow while writing an employment application.

• Choose professional words and humble toned words to make the letter look an expert-written. 
• Make sure you select an appropriate template. An appropriate template is a template that contains specified sections like introduction, letter body, and greetings with a suitable ending.
• Provide your personal information and mention the purpose of the letter. In this case, it would either be a cover letter or maybe verification of an employee’s job at a certain position.
• Write the main letter content, short but compact that should explain everything in detail. But make sure, you do not provide extra lengths and details that will make the letter look un-professional written. 
• End the letter with appropriate greetings. Provide your contact details in case the recipient wants to contact you back.

Sample job application letter with reference to newspaper ad:

We are providing you a sample and writing guidelines for employment letters. You can either choose this letter directly for your purpose or you may use the above-mentioned tips if you want the application all by yourself from scrape. Here is attached a sample letter:

Dear Sir/Madam {{Name}},

I am writing this letter in response to the advertisement which your company has published in leading newspapers regarding an open position of {{POSITION}}.

I have attached an updated copy along with this letter. Kindly spare some minutes to browse through my resume. I have also attached recommendation letters that I have earned over the years from my previous employers. 

Kindly let me know if you require any other detail. I will send it to you at the earliest. I can be reached at {{YOUR PHONE NUMBER}} or {{YOUR EMAIL ID}}.

Thank you very much for your consideration. I look forward to a positive response.

Yours sincerely,

Download this employment letter for free from our website. Do not forget to write us good feedback if you appreciate our efforts in making it easy for you. Head towards our website for some more useful and fruitful content. 

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dyaf teimi (1/27/2022)

nice sample

Cecil Chavez (2/12/2021) - NZL

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English Writing Exercises for A2 – An application letter

English Writing Exercises for A2

Preparation

1. put elements 1-6 in the correct places (a-f) in the letter..

1   the address the letter is going to

2   the writer’s name

3   the writer’s address

4   the date

5   the writer’s signature

6   the person the letter is going to

Dear Sir or Madam,

Application for the post of receptionist

I noticed the advertisement for a receptionist on your website and 1 ………… the post.

In the autumn I am going to study French and Spanish at university. 2 ………… a job where my languages will be useful.

3 ………… working in a hotel. Last summer 4 ………… a waiter in a hotel restaurant. Furthermore, I believe I possess the personal qualities necessary for the post. I am friendly and reliable. 5 ………… with guests in French and Spanish.

I am enclosing my CV and a reference from a teacher. 6 ………… start work on 1 July.

I look forward to hearing from you.

Yours faithfully,

1 B   2 F   3 C   4 D   5 E   6 A

2. Complete gaps 1-6 in the letter with these phrases.

a   I am looking for

b   I am also able to communicate

c   I have some experience of

d   I am writing to apply of

e   I will be able to

f   I worked as

1 d   2 a   3 c   4 f   5 b   6 e

3. Complete the summary of the letter.

The writer is applying for the job of 1 ……………………… . He saw the advert 2 ……………………… . He thinks that his 3 ……………………… will be useful for the job. He has experience of working as a 4 ……………………… . He thinks he is 5 ……………………… and 6 ……………………… . He is sending his 7 ……………………… and a reference with the letter and can start work on 8 ………………………

1 receptionist   2 on a website   3 languages

4 waiter   5 friendly   6 reliable

7 CV   8 1 July

Writing Guide

Writing Strategy

When you write a formal letter:

–  Start with: Dear ( Mrs Whilte ), if you know the name of the person, or Dear Sir or Madam , if you do not.

–  You can include a subject line at the start, similar to the subject line of an email.

–  Write in paragraphs. One-sentence paragraphs are fine for opening or closing an application letter.

–  Do not use colloquial language or short form ( I’m, there’s , etc.)

–  Finish with Yours sincerely , if you used the person’s name at the start, or Yours faithfully , if you did not.

4. Read the Writing Strategy above and the job advertisement below. Write a letter applying for the job. Follow the paragraph plan below.

Friendly and hard-working sales assistant required for busy music shop. July and August only. 9.00 a.m. – 5.30 p.m. six days a week. Apply enclosing your CV.

Paragraph plan

A   what the job is and how you found out about it

B   why you are interested in the job

C   why you are the right person for the job

D   what you are sending with the letter and when you can start work

E   what you would like to happen next

your own answers

5. Read the advertisement and write your application. Include the points below.

–  how you found out about the job.

–  your current situation

–  why you would be suitable

–  a request for further information

Do you like sport? Are you interested in working with children? We are looking for teenagers to help run sports and games for children during the summer holidays. Enthusiasm, a sense of fun and the ability to work well in a team are all important.

Please send you application by email to [email protected].

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View, manage, and install add-ins for Excel, PowerPoint, and Word

When you enable an add-in, it adds custom commands and new features to Microsoft 365 programs that help increase your productivity. Because add-ins can be used by attackers to do harm to your computer, you can use add-in security settings to help protect yourself.

Note:  This article only applies to Microsoft 365 applications running on Windows.

View installed add-ins

You can directly install add-ins from this page or select  More Add-ins  to explore.

In the Office Add-ins dialog, select  My Add-ins  tab.

Select an add-in you want to view the details for and right-click to select  Add-in details  option.

Click a heading below for more information .  

Add-in categories explained

Active Application Add-ins      Add-ins registered and currently running on your Microsoft 365 program.

Inactive Application Add-ins      These are present on your computer but not currently loaded. For example, XML schemas are active when the document that refers to them is open. Another example is the COM add-in: if a COM add-in is selected, the add-in is active. If the check box is cleared, the add-in is inactive.

Document Related Add-ins      Template files referred to by open documents.

Disabled Application Add-ins     These add-ins are automatically disabled because they are causing Microsoft 365 programs to crash.

Add-in      The title of the add-in.

Publisher      The software developer or organization responsible for creating the add-in.

Compatibility      Look here for any compatibility issues.

Location      This file path indicates where the add-in is installed on your computer.

Description This text explains the add-in function.

Note:  Microsoft Outlook has one add-in option in the Trust Center: Apply macro security settings to installed add-ins . InfoPath has no security settings for add-ins.

Permanently disable or remove an add-in

To disable or remove an add-in follow these steps:

Select  File > Get Add-ins . Alternatively, you can select  Home > Add-ins .

In the Office Add-ins dialog, select  My Add-ins  tab.

Select an add-in you want to remove and right click to select  Remove  option.

View or change add-in settings

You can see and change add-in settings in the Trust Center, descriptions of which are in the following section. Add-in security settings may have been determined by your organization so not all options may be available to change.

Select  File  >  Get Add-ins .

Select  More Add-ins > Manage My Add-ins.

Select  Trust Center  >  Trust Center Settings  >  Add-ins.

Check or uncheck the boxes you want.

Add-in settings explained

Require Application Add-ins to be signed by Trusted Publisher      Check this box to have the Trust Center check that the add-in uses a publisher's trusted signature. If the publisher's signature hasn’t been trusted, the Microsoft 365 program doesn’t load the add-in, and the Trust Bar displays a notification that the add-in has been disabled.

Disable notification for unsigned add-ins (code will remain disabled)      When you check the Require Application Extensions to be signed by Trusted Publisher box, this option is no longer grayed out. Add-ins signed by a trusted publisher are enabled, but unsigned add-ins are disabled.

Disable all Application Add-ins (may impair functionality)      Check this box if you don't trust any add-ins. All add-ins are disabled without any notification, and the other add-in boxes are grayed out.

Note:  This setting takes effect after you exit and restart your Microsoft 365 program.

While working with add-ins, you may need to learn more about digital signatures and certificates , which authenticate an add-in, and trusted publishers , the software developers who often create add-ins.

Manage and install add-ins

Use the following instruction to manage and install add-ins.

To install a new add-in:

You can directly install popular add-ins on the page or go to More Add-ins  to explore. 

Select the add-in and select  Add . Or browse by selecting  Store  tab in the Office add-in dialog to find other add-ins to install and select Add for that add-in.

To manage your add-ins:

Select  File > Get Add-ins and from the bottom, select More Add-ins.  Or select  Home  >  Add-ins > More add-ins.

In the Office dialog, select My Add-ins tab. If you are not able to see your add-ins, select  Refresh to reload your add-ins.

Select  Manage My Add-in  to manage and select  Upload to browse and add an add-in from your device.

How to cancel a purchased add-in

If you've subscribed to an add-in through the Microsoft 365 Store that you don't want to continue, you can cancel that subscription.

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• Published: 23 April 2024

Therapeutic application of circular RNA aptamers in a mouse model of psoriasis

• Si-Kun Guo   ORCID: orcid.org/0000-0003-1637-5299 1   na1 ,
• Chu-Xiao Liu   ORCID: orcid.org/0000-0002-6725-8327 1   na1 ,
• Yi-Feng Xu   ORCID: orcid.org/0000-0002-5111-8565 1   na1 ,
• Xiao Wang 1   na1 ,
• Fang Nan 2   na1 ,
• Youkui Huang 1 ,
• Siqi Li 1 ,
• Shan Nan 1 ,
• Ling Li 1 , 3 ,
• Edo Kon 4 ,
• Chen Li 1 ,
• Meng-Yuan Wei 1 ,
• Rina Su 5 ,
• Jia Wei 2 ,
• Shiguang Peng 5 ,
• Nitay Ad-El   ORCID: orcid.org/0000-0002-1936-8628 4 ,
• Jiaquan Liu 1 ,
• Dan Peer   ORCID: orcid.org/0000-0001-8238-0673 4 ,
• Ting Chen   ORCID: orcid.org/0000-0002-7404-4538 6 ,
• Li Yang   ORCID: orcid.org/0000-0001-8833-7473 2 &
• Ling-Ling Chen   ORCID: orcid.org/0000-0001-9501-0305 1 , 3 , 7 , 8  

Nature Biotechnology ( 2024 ) Cite this article

Metrics details

• Long non-coding RNAs
• Nucleic-acid therapeutics

Efforts to advance RNA aptamers as a new therapeutic modality have been limited by their susceptibility to degradation and immunogenicity. In a previous study, we demonstrated synthesized short double-stranded region-containing circular RNAs (ds-cRNAs) with minimal immunogenicity targeted to dsRNA-activated protein kinase R (PKR). Here we test the therapeutic potential of ds-cRNAs in a mouse model of imiquimod-induced psoriasis. We find that genetic supplementation of ds-cRNAs leads to inhibition of PKR, resulting in alleviation of downstream interferon-α and dsRNA signals and attenuation of psoriasis phenotypes. Delivery of ds-cRNAs by lipid nanoparticles to the spleen attenuates PKR activity in examined splenocytes, resulting in reduced epidermal thickness. These findings suggest that ds-cRNAs represent a promising approach to mitigate excessive PKR activation for therapeutic purposes.

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Data availability

All data supporting the findings of this study are available in the paper, Source Data and at https://doi.org/10.17632/zfm9kmfghs.2 (ref. 77 ). All sequencing data reported in this paper have been deposited in the Gene Expression Omnibus (GEO). High-throughput datasets generated in this study are available at GSE248680 (ref. 78 ), including circSHAPE-MaP data ( GSE248679 ) and mouse RNA-seq data (IMQ-treated Pkr −/− mouse data, GSE248678 ; mouse spleens delivered EPIC-LNPs data, GSE253346 ); published RNA-seq data of patients with psoriasis can be downloaded from GEO under accession number GSE121212 (ref. 79 ). Source data are provided with this paper.

Code availability

This paper does not report original code. Any additional information required to reanalyze the data reported in this paper is available from the lead contact on request.

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Acknowledgements

We thank F. Nan at Shanghai Institute of Materia Medica for support in LNPs, and the Chen laboratory members for critical discussion. This work was supported by National Key R&D Program of China (grant no. 2021YFA1300501), Strategic Priority Research Program of the Chinese Academy of Science (grant no. XDB0570000) and Science and Technology Commission of Shanghai Municipality (STCSM) (grant nos. 23DX1900100 and 23DX1900101) to L.-L.C.; National Natural Science Foundation of China (NSFC) (grant no. 31925011), National Key R&D Program of China (grant nos. 2021YFA1300503 and 2019YFA0802804) and STCSM (grant nos. 23DX1900102 and 23JS1400300) to L.Y.; NSFC (grant no. 32371349) and Shanghai Rising-Star Program (grant no. 23QA1410200) to C.-X.L.; China National Postdoctoral Program for Innovative Talents (grant nos. BX20220298 and BX20220077) and Shanghai Postdoctoral Excellence Program (grant nos. 2022757 and 2022728) to X.W. and F.N. D.P. acknowledges the support from the European Research Council (advance grant no. 101055029). This work has been supported by the New Cornerstone Science Foundation through the New Cornerstone Investigator Program and the XPLORER PRIZE.

Author information

These authors contributed equally: Si-Kun Guo, Chu-Xiao Liu, Yi-Feng Xu, Xiao Wang, Fang Nan.

Authors and Affiliations

Key Laboratory of RNA Innovation, Science and Engineering, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai, China

Si-Kun Guo, Chu-Xiao Liu, Yi-Feng Xu, Xiao Wang, Youkui Huang, Siqi Li, Shan Nan, Ling Li, Chen Li, Meng-Yuan Wei, Jiaquan Liu & Ling-Ling Chen

Center for Molecular Medicine, Children’s Hospital of Fudan University and Shanghai Key Laboratory of Medical Epigenetics, International Laboratory of Medical Epigenetics and Metabolism, Ministry of Science and Technology, Institutes of Biomedical Sciences, Fudan University, Shanghai, China

Fang Nan, Jia Wei & Li Yang

School of Life Science and Technology, ShanghaiTech University, Shanghai, China

Ling Li & Ling-Ling Chen

Laboratory of Precision Nanomedicine, Shmunis School of Biomedicine and Cancer Research, George S. Wise Faculty of Life Sciences, Department of Materials Sciences and Engineering, Iby and Aladar Fleischman Faculty of Engineering, Center for Nanoscience and Nanotechnology, Cancer Biology Research Center, Tel Aviv University, Tel Aviv, Israel

Edo Kon, Nitay Ad-El & Dan Peer

Department of Dermatology, Beijing Chao-yang Hospital, Capital Medical University, Beijing, China

Rina Su & Shiguang Peng

National Institute of Biological Sciences, Tsinghua Institute of Multidisciplinary Biomedical Research, Tsinghua University, Beijing, China

New Cornerstone Science Laboratory, Shenzhen, China

Ling-Ling Chen

Key Laboratory of Systems Health Science of Zhejiang Province, School of Life Science, Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Hangzhou, China

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Contributions

L.-L.C. supervised and conceived the project. S.-K.G., C.-X.L., Y.-F.X. and X.W. designed and performed experiments. F.N. preformed computational analyses, supervised by L.Y. Y.H., S.L., L.L., E.K. and N.A. formulated LNPs, supervised by D.P. and L.-L.C. R.S. and S.P. provided samples from patients with psoriasis, supervised by T.C. C.L. helped with smTIRF experiments, supervised by J.L. J.W. generated the next-generation sequencing library. S.N. and M.-Y.W. helped with biochemical and mice experiments. L.-L.C., S.-K.G., C.-X.L., Y.-F.X., X.W. and F.N. wrote the paper with input from all authors. All authors read and approved the manuscript.

Corresponding author

Correspondence to Ling-Ling Chen .

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Competing interests.

L.-L.C., S.-K.G., C.-X.L., S.L. and Y.-F.X. are named as inventors on patents related to circRNA held by CAS CEMCS. L.-L.C. is a scientific co-founder of RiboX Therapeutics. D.P. receives licensing fees (to patents on which he was an inventor) from, invested in, consults (or on scientific advisory boards or boards of directors) for, lectured (and received a fee) or conducts sponsored research at TAU for the following entities: ART Biosciences, BioNtech SE, Earli Inc., Kernal Biologics, Geneditor Biologics, Newphase Ltd, NeoVac Ltd, RiboX Therapeutics, Roche, SirTLabs Corporation and Teva Pharmaceuticals Inc.

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Extended data

Extended data fig. 1 epic synthesized by optimized strategy preserves its characteristics in minimized immunogenicity and folding status..

( a ) Examination of circularization efficiency of two circular RNAs (circPOLR2A, 336 nt; circmCherry, 1452 nt) using Anabaena tRNA Leu -derived PIE with varied lengths of extraneous nucleotides. Circularized RNA products are analyzed with denaturing PAGE, bands of RNA circles are verified with RNase R and marked with blue arrows. Representative results are shown from three replicates. Gels of each replicate are processed in parallel. ( b ) The 27 nt extraneous sequence tend to form stem-loop alone at the JS, independent of cargo sequences by predictions of in silico and SHAPE-MaP. ( c ) Ana_PIE_27nt version results in minimal induction of inflammatory factors. The same amounts of circular POLR2A (200 ng for each sample) with varied lengths of extraneous nucleotides were transfected into A549 cells. Relative expression of inflammatory factors after 6 hours transfection were examined by RT-qPCR. n  = 3 biological repeats. ( d ) Secondary structures of circPOLR2A_Lig and circPOLR2A_J (EPIC). Left, the original and new junction sites are indicated in the secondary structure of circPOLR2A_Lig 8 . Right, the secondary structure of circPOLR2A_J (EPIC), in which the 27 nt extraneous sequences forms a stable step-loop. The predicted imperfect duplex regions are marked with gray and yellow shadows. ( e ) Human PKR protein purified from E. coli is shown by SDS-PAGE and Coomassie Blue staining. ( f ) The 27 extra nucleotides RNA circle doesn’t suppress PKR phosphorylation. Purified PKR (0.6 μM) is activated by 79 bp dsRNA (0.01 μM) in vitro, which is shown by autoradiography using γ- 32 P-ATP. 0.01 μM of RNA circles are used in the assays. ( g ) EPIC suppresses mouse PKR phosphorylation efficiently. Left, purified mouse PKR protein is shown by SDS-PAGE and Coomassie Blue staining. Right, the activation of mPkr (0.6 μM) by 79 bp dsRNA (0.01 μM) in vitro, is inhibited by EPIC (0.01 μM). c , f and g : n.s., p > 0.05, *p < 0.05, **p < 0.005, ***p < 0.001, two-tailed student’s t test, data are shown as mean ± SD.

Extended Data Fig. 2 Stable interactions of ds-cRNAs and PKR resolved by single molecule imaging.

( a ) Cy5 and biotin labeling did not alter the EPIC conformation in general. SHAPE reactivity of each group was quantified at the single nucleotide resolution, and ΔSHAPE was calculated accordingly and plotted in absolute values. ( b ) The binding frequency between PKR and examined RNAs by smTIRF. The numbers of RNAs counted in each column with three independent replicates: 79 bp dsRNA, n  = 265, 108, 157; 33 bp dsRNA, n  = 204, 179, 135; 73 nt hairpin RNA, n  = 207, 281, 394; 23 bp duplex RNA, n  = 178, 277, 363; EPIC, n  = 134, 150, 178; circSMARCA5, n  = 67, 101, 78. The binding frequency of PKR on each RNA: 79 bp dsRNA, 62.9%; 33 bp dsRNA, 37.3%; 73 nt hairpin RNA, 39.4%; 23 bp duplex RNA, 11.6%; EPIC, 22.4%; circSMARCA5, 1.5%. Data are shown as mean ± SD. ( c ) A similar robust binding between PKR and circCAMSAP1. Left, representative kymograph of circCAMSAP1-Cy5 binding with PKR-Cy3 in separated and merged channels. Right: quantification of the PKR-Cy3 intensity on a single RNA molecule within 120 s. ( d ) HMM has been applied to analysis single-step photobleaching data. Trace line represents raw PKR-Cy3 trajectory of one exemplary molecule recorded by smTIRF. Means line represents the idealized trajectory determined by HMM. ( e ) The interaction between EPIC and PKR-ΔIDR is similar with WT PKR. Left, the construction of WT PKR and PKR-ΔIDR. Middle, the binding frequency between EPIC and PKR (or PKR-ΔIDR). The numbers of RNAs counted in each column with three independent replicates: PKR, n  = 287, 201, 350; PKR-ΔIDR, n  = 237, 371, 299. Data are shown as mean ± SD. Right, the number of PKR-ΔIDR binding on single EPIC is calculated based on the step analysis of PKR-ΔIDR-Cy3 photobleaching. n  = number of events examined.

Extended Data Fig. 3 Chemical probing and docked structure reveal the potential binding mode between one EPIC with two PKR molecules.

( a ) The SHAPE signal difference (ΔSHAPE value) of EPIC with or without PKR protein in vitro. Top, SHAPE reactivity of each group is quantified. Bottom, ΔSHAPE value of EPIC with or without PKR protein in vitro. Yellow shadow indicates the nucleotides of 23 bp imperfect dsRNA-region. Regions with significant SHAPE augment upon PKR binding are in purple, regions with reduced SHAPE signals upon PKR binding are in green and marked by pink shadow. ( b ) Predicted secondary structure of EPIC based on circSHAPE-MaP signals. Of note, EPIC contains one imperfect dsRNA region sufficient for PKR binding (yellow shadow, 23 bp). The heavy pink lines denote the altered NAI accessibility in EPIC upon PKR addition. ( c ) Predicted EPIC structure and the binding with PKR. Top left, domains of PKR and the schematic pipeline for docking EPIC on PKR. Bottom left, the most possible 3D structure of EPIC predicted by 2D structure with circSHAPE-MaP signal. Right, the binding modes of 79 bp dsRNA (top) and EPIC (bottom) docked with two PKR molecules predicted by AlphaFold2. The 23 bp imperfect dsRNA region is labeled in yellow, and the nucleotides with altered SHAPE reactivities upon PKR binding are labeled in red.

Extended Data Fig. 4 CircRNAs are globally degraded at the initial inflammation stage in IMQ-induced psoriasis mice model.

( a ) Phenotypes of IMQ-induced psoriasis mice model. Left, representative image of mice showing skin inflammation after IMQ treatment on dorsal skin, compared to Vaseline treatment or blank control. Right, quantification of the spleen size at indicated time points upon IMQ treatment. n  = 4 or 5 animals in each group. ( b ) Increased expression of psoriasis-related genes with distinct patterns. Relative expression levels of the psoriasis-related genes (mIl17a and mIl23a), mPkr and mIl6 were examined by RT-qPCR. The expression is normalized to D0 expression. n  = 4 or 5 animals in each group and mActin is used as an internal control for normalization. ( c ) Activation of RNase L was measured by RT-qPCR combined with RctB ligation. ( d ) Increased epidermis thickness of dorsal skins from IMQ-treated mice. n  = 3 animals in each group. ( e ) Expression level of 721 HC circRNAs and their cognate linear RNAs in IMQ treated mice. The value of each time point is the mean from two repeats, and each repeat includes 2 biologically independent animals. Data are shown as median and IQR. Wilcoxon rank-sum test, ***p < 0.001. ( f ) Validation of randomly selected circRNAs, their cognate mRNAs and pre-mRNAs at indicated time points in IMQ treated mice. Expression of these RNAs were examined by RT-qPCR and normalized to mActin. Data are shown as mean ± SD.

Extended Data Fig. 5 Enrichment analyses reveal up-regulated pathways in the initial stage of psoriasis pathogenesis.

( a ) Schematic of clustering the fast-responding inflammatory factors including PKR. 787 DEGs are defined as over threefold up-regulation at D1 compared to D0 in two biological repeats. All DEGs are performed the KEGG pathway enrichment analysis. ( b ) The KEGG analyses of 151 genes from Cluster 1. ( c ) A short list of the most enriched genes of Cluster 1 which belong to the IFNα signaling pathways and the dsRNA-sensors related genes. ( d )-( h ) The enriched KEGG pathways of genes from the other 5 clusters of distinct gene expression patterns in IMQ-induced psoriasis mice model. b , d , e , f , g , h : all pathways are selected according p.adjust < 0.05 and the pathways related to viral or bacterial infection are not shown in Cluster 1.

Extended Data Fig. 6 Generation of Pkr -/- mice.

( a ) Generation of Pkr-/- mice by CRISPR/Cas9. Top, the schematic of generating Pkr-/- mice. Bottom left, the wiggle track of RNA-seq data of total RNAs collected from WT and Pkr-/- mice spleens. Bottom right, WB verification of mPkr loss. ( b ) The expression of circRNAs and their cognate linear RNAs in Pkr-/- mice with IMQ treatment. FPBcirc values (top), FPBlinear value (middle) and CIRCscore (bottom) of 721 HC circRNAs in each indicated time points, related to Fig. 3c and Extended Data Fig. 4e . Each corresponding group is calculated mean from two biological repeats, and each repeat with 2 biologically independent animals. Data are shown as median and IQR. Wilcoxon rank-sum test, ***p < 0.001. ( c ) Heatmap of selected categories of psoriasis-related gene expression from the IMQ-treated spleens of Pkr-/- and paired WT mice. Each repeat includes two biologically independent animals. The expression of each gene is scaled by z-score and each category is shown in the same scale bar, indicated by intervals.

Extended Data Fig. 7 Generation of the in vivo over-expressed human circPOLR2A (9,10) mice model (C_OE mice).

( a ) Schematic of generating C_OE mice carrying the circPOLR2A (9,10) constitutive expression cassette. ( b ) NB analysis showed constitutive human circPOLR2A (9, 10) production in all examined tissues in the EF1α promoter and CAG promoter driven C_OE mice. ( c ) NB analysis confirmed the expression of human circPOLR2A (9, 10) in the liver of EF1α and CAG C_OE mice, respectively. ( d ) EF1α C_OE mice exhibited unobservable abnormalities compared to WT mice, here showing the weight analysis of WT and the littermate EF1α C_OE mice from 4 to 60 weeks. Each dot represents one individual animal ( n  ≥ 3) at each examined time point. n.s., p > 0.05, two-tailed student’s t test, data are shown as mean ± SD. ( e ) Generation of the in vivo over-expressed human circPOLR2A (9,10) R1 cell lines. ( f ) Schematic of calculating the copy number of human circPOLR2A (9, 10) in C_OE mice, relative to OE_C R1 cells in e .

Extended Data Fig. 8 In vivo over-expression of the human circPOLR2A (9, 10) mitigates the IMQ-induced psoriasis pathogenesis.

( a ) Human circPOLR2A (9, 10) dampens the expression of inflammatory factors in IMQ mice. Relative expression of representative IFNα signaling pathways in C_OE mice ( n  = 6), compared to WT mice ( n  = 5) on D1, examined by RT-qPCR. n.s., p > 0.05, *p < 0.05, **p < 0.005, two-tailed student’s t test, data are shown as mean ± SD. ( b ) Human circPOLR2A (9, 10) dampens PKR activation in IMQ mice. PKR activation kinetics were analyzed in C_OE and WT mice on D2, ActB was used as the internal control. ( c ) RNase L activation on D1 led to reduced circPOLR2A (9,10) in C_OE mice. ( d ) Relative expression levels of the circPOLR2A (9,10) were examined by RT-qPCR in IMQ mice. The expression of circPOLR2A (9,10) is normalized to D0 and mActin is used as an internal control for normalization.

Extended Data Fig. 9 Ds-cRNAs are encapsulated by ionizable LNPs.

( a ) Schematic of a microfluidic mixing preparation of circular RNA-encapsulated ionizable-based LNPs. ( b ) Physicochemical characterization of ds-cRNA-encapsulated ionizable-based LNPs. The particle size, PDI, Zeta potential and encapsulation efficiency of EPIC-LNPs were measured in three replicates. Data are shown as mean ± SD. ( c ) Transmission electron micrographs of EPIC-LNPs. ( d ) Examination of endogenous circRNA expression upon ds-cRNA treatment by RNA-seq. Blue dots, quantification of total RNAs from spleens of IMQ mice without EPIC-LNPs. Orange triangles, quantification of total RNAs from spleens at 24 hrs post i.v. of 150 pmol EPIC-LNPs at D1.5 IMQ mice; of note, EPICs were counted by FPBcirc in these samples. Blue triangles, quantification of total endogenous RNAs from same samples shown by Orange triangles, but leaving out EPICs during calculation by FPBcirc. EPIC-LNPs treatment restored the reduced endogenous circRNA almost to the D0 level.

Extended Data Fig. 10 Expression of fast-responding inflammatory factors is elevated in psoriasis patients and the ds-cRNA treatment is beneficial in patient-derived PBMCs.

( a ) The expression of circRNAs is reduced in the lesional skin samples, compared to the non-lesional skin samples. Total expression level of circRNAs from lesional and non-lesional skin samples derived from 27 psoriasis patients 19 , 20 are analyzed. Top, total FPBcirc values of all circRNAs; median, total FPBlinear values of all circRNAs cognate linear RNAs; bottom, total CIRCscore values of all circRNAs. Wilcoxon rank-sum test, n.s., p > 0.05, ***p < 0.001. ( b ) Heatmap of the psoriasis-related genes of lesional and non-lesional skin in psoriasis patients. The expression level of each gene is scaled by z-score and each category is shown in the same scale bar, indicated by intervals. ( c ) EPIC-LNPs suppresses the expression of psoriatic signature genes in PBMCs isolated from psoriasis patients. 1 pmol of EPIC was delivered into roughly 10 6 cells. Each dot represents one patient sample. n.s., p > 0.05, *p < 0.05, paired student’s t test.

Supplementary information

Reporting summary, supplementary table 1.

The circRNA expression in IMQ-induced psoriasis mice.

Supplementary Table 2

The linear RNA expression in IMQ-induced psoriasis mice.

Supplementary Table 3

Gene expression cluster and KEGG pathway in IMQ-induced psoriasis mice.

Supplementary Table 4

RNA-seq profile of patients with psoriasis (linear RNA).

Supplementary Table 5

RNA-seq profile of patients with psoriasis (circRNA).

Supplementary Table 6

General information of psoriasis donors.

Supplementary Table 7

List of construct, probe and primer sequences.

Supplementary Table 8

Endogenous circRNA expression of psoriasis mice delivered EPIC-LNPs.

Source data

Source data fig. 1.

Unprocessed western blots and/or gels associated with the data presented in main and extended data figures.

Source Data Fig. 2

Statistical source data associated with the data presented in main and extended data figures.

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Guo, SK., Liu, CX., Xu, YF. et al. Therapeutic application of circular RNA aptamers in a mouse model of psoriasis. Nat Biotechnol (2024). https://doi.org/10.1038/s41587-024-02204-4

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Received : 20 October 2023

Accepted : 12 March 2024

Published : 23 April 2024

DOI : https://doi.org/10.1038/s41587-024-02204-4

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