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Hypothesis Testing | A Step-by-Step Guide with Easy Examples

Published on November 8, 2019 by Rebecca Bevans . Revised on June 22, 2023.

Hypothesis testing is a formal procedure for investigating our ideas about the world using statistics . It is most often used by scientists to test specific predictions, called hypotheses, that arise from theories.

There are 5 main steps in hypothesis testing:

State your research hypothesis as a null hypothesis and alternate hypothesis (H o ) and (H a or H 1 ).
Collect data in a way designed to test the hypothesis.
Perform an appropriate statistical test .
Decide whether to reject or fail to reject your null hypothesis.
Present the findings in your results and discussion section.

Though the specific details might vary, the procedure you will use when testing a hypothesis will always follow some version of these steps.

Step 1: state your null and alternate hypothesis, step 2: collect data, step 3: perform a statistical test, step 4: decide whether to reject or fail to reject your null hypothesis, step 5: present your findings, other interesting articles, frequently asked questions about hypothesis testing.

After developing your initial research hypothesis (the prediction that you want to investigate), it is important to restate it as a null (H o ) and alternate (H a ) hypothesis so that you can test it mathematically.

The alternate hypothesis is usually your initial hypothesis that predicts a relationship between variables. The null hypothesis is a prediction of no relationship between the variables you are interested in.

H 0 : Men are, on average, not taller than women. H a : Men are, on average, taller than women.

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For a statistical test to be valid , it is important to perform sampling and collect data in a way that is designed to test your hypothesis. If your data are not representative, then you cannot make statistical inferences about the population you are interested in.

There are a variety of statistical tests available, but they are all based on the comparison of within-group variance (how spread out the data is within a category) versus between-group variance (how different the categories are from one another).

If the between-group variance is large enough that there is little or no overlap between groups, then your statistical test will reflect that by showing a low p -value . This means it is unlikely that the differences between these groups came about by chance.

Alternatively, if there is high within-group variance and low between-group variance, then your statistical test will reflect that with a high p -value. This means it is likely that any difference you measure between groups is due to chance.

Your choice of statistical test will be based on the type of variables and the level of measurement of your collected data .

an estimate of the difference in average height between the two groups.
a p -value showing how likely you are to see this difference if the null hypothesis of no difference is true.

Based on the outcome of your statistical test, you will have to decide whether to reject or fail to reject your null hypothesis.

In most cases you will use the p -value generated by your statistical test to guide your decision. And in most cases, your predetermined level of significance for rejecting the null hypothesis will be 0.05 – that is, when there is a less than 5% chance that you would see these results if the null hypothesis were true.

In some cases, researchers choose a more conservative level of significance, such as 0.01 (1%). This minimizes the risk of incorrectly rejecting the null hypothesis ( Type I error ).

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The results of hypothesis testing will be presented in the results and discussion sections of your research paper , dissertation or thesis .

In the results section you should give a brief summary of the data and a summary of the results of your statistical test (for example, the estimated difference between group means and associated p -value). In the discussion , you can discuss whether your initial hypothesis was supported by your results or not.

In the formal language of hypothesis testing, we talk about rejecting or failing to reject the null hypothesis. You will probably be asked to do this in your statistics assignments.

However, when presenting research results in academic papers we rarely talk this way. Instead, we go back to our alternate hypothesis (in this case, the hypothesis that men are on average taller than women) and state whether the result of our test did or did not support the alternate hypothesis.

If your null hypothesis was rejected, this result is interpreted as “supported the alternate hypothesis.”

These are superficial differences; you can see that they mean the same thing.

You might notice that we don’t say that we reject or fail to reject the alternate hypothesis . This is because hypothesis testing is not designed to prove or disprove anything. It is only designed to test whether a pattern we measure could have arisen spuriously, or by chance.

If we reject the null hypothesis based on our research (i.e., we find that it is unlikely that the pattern arose by chance), then we can say our test lends support to our hypothesis . But if the pattern does not pass our decision rule, meaning that it could have arisen by chance, then we say the test is inconsistent with our hypothesis .

If you want to know more about statistics , methodology , or research bias , make sure to check out some of our other articles with explanations and examples.

Normal distribution
Descriptive statistics
Measures of central tendency
Correlation coefficient

Methodology

Cluster sampling
Stratified sampling
Types of interviews
Cohort study
Thematic analysis

Research bias

Implicit bias
Cognitive bias
Survivorship bias
Availability heuristic
Nonresponse bias
Regression to the mean

Hypothesis testing is a formal procedure for investigating our ideas about the world using statistics. It is used by scientists to test specific predictions, called hypotheses , by calculating how likely it is that a pattern or relationship between variables could have arisen by chance.

A hypothesis states your predictions about what your research will find. It is a tentative answer to your research question that has not yet been tested. For some research projects, you might have to write several hypotheses that address different aspects of your research question.

A hypothesis is not just a guess — it should be based on existing theories and knowledge. It also has to be testable, which means you can support or refute it through scientific research methods (such as experiments, observations and statistical analysis of data).

Null and alternative hypotheses are used in statistical hypothesis testing . The null hypothesis of a test always predicts no effect or no relationship between variables, while the alternative hypothesis states your research prediction of an effect or relationship.

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7.1: Basics of Hypothesis Testing

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Page ID 16360

Kathryn Kozak
Coconino Community College

To understand the process of a hypothesis tests, you need to first have an understanding of what a hypothesis is, which is an educated guess about a parameter. Once you have the hypothesis, you collect data and use the data to make a determination to see if there is enough evidence to show that the hypothesis is true. However, in hypothesis testing you actually assume something else is true, and then you look at your data to see how likely it is to get an event that your data demonstrates with that assumption. If the event is very unusual, then you might think that your assumption is actually false. If you are able to say this assumption is false, then your hypothesis must be true. This is known as a proof by contradiction. You assume the opposite of your hypothesis is true and show that it can’t be true. If this happens, then your hypothesis must be true. All hypothesis tests go through the same process. Once you have the process down, then the concept is much easier. It is easier to see the process by looking at an example. Concepts that are needed will be detailed in this example.

Example $\PageIndex{1}$ basics of hypothesis testing

Suppose a manufacturer of the XJ35 battery claims the mean life of the battery is 500 days with a standard deviation of 25 days. You are the buyer of this battery and you think this claim is inflated. You would like to test your belief because without a good reason you can’t get out of your contract.

What do you do?

Well first, you should know what you are trying to measure. Define the random variable.

Let x = life of a XJ35 battery

Now you are not just trying to find different x values. You are trying to find what the true mean is. Since you are trying to find it, it must be unknown. You don’t think it is 500 days. If you did, you wouldn’t be doing any testing. The true mean, $\mu$, is unknown. That means you should define that too.

Let $\mu$= mean life of a XJ35 battery

You may want to collect a sample. What kind of sample?

You could ask the manufacturers to give you batteries, but there is a chance that there could be some bias in the batteries they pick. To reduce the chance of bias, it is best to take a random sample.

How big should the sample be?

A sample of size 30 or more means that you can use the central limit theorem. Pick a sample of size 30.

Example $\PageIndex{1}$ contains the data for the sample you collected:

Now what should you do? Looking at the data set, you see some of the times are above 500 and some are below. But looking at all of the numbers is too difficult. It might be helpful to calculate the mean for this sample.

The sample mean is $\overline{x} = 490$ days. Looking at the sample mean, one might think that you are right. However, the standard deviation and the sample size also plays a role, so maybe you are wrong.

Before going any farther, it is time to formalize a few definitions.

You have a guess that the mean life of a battery is less than 500 days. This is opposed to what the manufacturer claims. There really are two hypotheses, which are just guesses here – the one that the manufacturer claims and the one that you believe. It is helpful to have names for them.

Definition $\PageIndex{1}$

Null Hypothesis : historical value, claim, or product specification. The symbol used is $H_{o}$.

Definition $\PageIndex{2}$

Alternate Hypothesis : what you want to prove. This is what you want to accept as true when you reject the null hypothesis. There are two symbols that are commonly used for the alternative hypothesis: $H_{A}$ or $H_{I}$. The symbol $H_{A}$ will be used in this book.

In general, the hypotheses look something like this:

$H_{o} : \mu=\mu_{o}$

$H_{A} : \mu<\mu_{o}$

where $\mu_{o}$ just represents the value that the claim says the population mean is actually equal to.

Also, $H_{A}$ can be less than, greater than, or not equal to.

For this problem:

$H_{o} : \mu=500$ days, since the manufacturer says the mean life of a battery is 500 days.

$H_{A} : \mu<500$ days, since you believe that the mean life of the battery is less than 500 days.

Now back to the mean. You have a sample mean of 490 days. Is this small enough to believe that you are right and the manufacturer is wrong? How small does it have to be?

If you calculated a sample mean of 235, you would definitely believe the population mean is less than 500. But even if you had a sample mean of 435 you would probably believe that the true mean was less than 500. What about 475? Or 483? There is some point where you would stop being so sure that the population mean is less than 500. That point separates the values of where you are sure or pretty sure that the mean is less than 500 from the area where you are not so sure. How do you find that point?

Well it depends on how much error you want to make. Of course you don’t want to make any errors, but unfortunately that is unavoidable in statistics. You need to figure out how much error you made with your sample. Take the sample mean, and find the probability of getting another sample mean less than it, assuming for the moment that the manufacturer is right. The idea behind this is that you want to know what is the chance that you could have come up with your sample mean even if the population mean really is 500 days.

You want to find $P\left(\overline{x}<490 | H_{o} \text { is true }\right)=P(\overline{x}<490 | \mu=500)$

To compute this probability, you need to know how the sample mean is distributed. Since the sample size is at least 30, then you know the sample mean is approximately normally distributed. Remember $\mu_{\overline{x}}=\mu$ and $\sigma_{\overline{x}}=\dfrac{\sigma}{\sqrt{n}}$

A picture is always useful.

Before calculating the probability, it is useful to see how many standard deviations away from the mean the sample mean is. Using the formula for the z-score from chapter 6, you find

$z=\dfrac{\overline{x}-\mu_{o}}{\sigma / \sqrt{n}}=\dfrac{490-500}{25 / \sqrt{30}}=-2.19$

This sample mean is more than two standard deviations away from the mean. That seems pretty far, but you should look at the probability too.

On TI-83/84:

$P(\overline{x}<490 | \mu=500)=\text { normalcdf }(-1 E 99,490,500,25 \div \sqrt{30}) \approx 0.0142$

$P(\overline{x}<490 \mu=500)=\text { pnorm }(490,500,25 / \operatorname{sqrt}(30)) \approx 0.0142$

There is a 1.42% chance that you could find a sample mean less than 490 when the population mean is 500 days. This is really small, so the chances are that the assumption that the population mean is 500 days is wrong, and you can reject the manufacturer’s claim. But how do you quantify really small? Is 5% or 10% or 15% really small? How do you decide?

Before you answer that question, a couple more definitions are needed.

Definition $\PageIndex{3}$

Test Statistic : $z=\dfrac{\overline{x}-\mu_{o}}{\sigma / \sqrt{n}}$ since it is calculated as part of the testing of the hypothesis.

Definition $\PageIndex{4}$

p – value : probability that the test statistic will take on more extreme values than the observed test statistic, given that the null hypothesis is true. It is the probability that was calculated above.

Now, how small is small enough? To answer that, you really want to know the types of errors you can make.

There are actually only two errors that can be made. The first error is if you say that $H_{o}$ is false, when in fact it is true. This means you reject $H_{o}$ when $H_{o}$ was true. The second error is if you say that $H_{o}$ is true, when in fact it is false. This means you fail to reject $H_{o}$ when $H_{o}$ is false. The following table organizes this for you:

Type of errors:

Definition $\PageIndex{5}$

Type I Error is rejecting $H_{o}$ when $H_{o}$ is true, and

Definition $\PageIndex{6}$

Type II Error is failing to reject $H_{o}$ when $H_{o}$ is false.

Since these are the errors, then one can define the probabilities attached to each error.

Definition $\PageIndex{7}$

$\alpha$ = P(type I error) = P(rejecting $H_{o} / H_{o}$ is true)

Definition $\PageIndex{8}$

$\beta$ = P(type II error) = P(failing to reject $H_{o} / H_{o}$ is false)

$\alpha$ is also called the level of significance .

Another common concept that is used is Power = $1-\beta $.

Now there is a relationship between $\alpha$ and $\beta$. They are not complements of each other. How are they related?

If $\alpha$ increases that means the chances of making a type I error will increase. It is more likely that a type I error will occur. It makes sense that you are less likely to make type II errors, only because you will be rejecting $H_{o}$ more often. You will be failing to reject $H_{o}$ less, and therefore, the chance of making a type II error will decrease. Thus, as $\alpha$ increases, $\beta$ will decrease, and vice versa. That makes them seem like complements, but they aren’t complements. What gives? Consider one more factor – sample size.

Consider if you have a larger sample that is representative of the population, then it makes sense that you have more accuracy then with a smaller sample. Think of it this way, which would you trust more, a sample mean of 490 if you had a sample size of 35 or sample size of 350 (assuming a representative sample)? Of course the 350 because there are more data points and so more accuracy. If you are more accurate, then there is less chance that you will make any error. By increasing the sample size of a representative sample, you decrease both $\alpha$ and $\beta$.

Summary of all of this:

For a certain sample size, n , if $\alpha$ increases, $\beta$ decreases.
For a certain level of significance, $\alpha$, if n increases, $\beta$ decreases.

Now how do you find $\alpha$ and $\beta$? Well $\alpha$ is actually chosen. There are only three values that are usually picked for $\alpha$: 0.01, 0.05, and 0.10. $\beta$ is very difficult to find, so usually it isn’t found. If you want to make sure it is small you take as large of a sample as you can afford provided it is a representative sample. This is one use of the Power. You want $\beta$ to be small and the Power of the test is large. The Power word sounds good.

Which pick of $\alpha$ do you pick? Well that depends on what you are working on. Remember in this example you are the buyer who is trying to get out of a contract to buy these batteries. If you create a type I error, you said that the batteries are bad when they aren’t, most likely the manufacturer will sue you. You want to avoid this. You might pick $\alpha$ to be 0.01. This way you have a small chance of making a type I error. Of course this means you have more of a chance of making a type II error. No big deal right? What if the batteries are used in pacemakers and you tell the person that their pacemaker’s batteries are good for 500 days when they actually last less, that might be bad. If you make a type II error, you say that the batteries do last 500 days when they last less, then you have the possibility of killing someone. You certainly do not want to do this. In this case you might want to pick $\alpha$ as 0.10. If both errors are equally bad, then pick $\alpha$ as 0.05.

The above discussion is why the choice of $\alpha$ depends on what you are researching. As the researcher, you are the one that needs to decide what $\alpha$ level to use based on your analysis of the consequences of making each error is.

If a type I error is really bad, then pick $\alpha$ = 0.01.

If a type II error is really bad, then pick $\alpha$ = 0.10

If neither error is bad, or both are equally bad, then pick $\alpha$ = 0.05

The main thing is to always pick the $\alpha$ before you collect the data and start the test.

The above discussion was long, but it is really important information. If you don’t know what the errors of the test are about, then there really is no point in making conclusions with the tests. Make sure you understand what the two errors are and what the probabilities are for them.

Now it is time to go back to the example and put this all together. This is the basic structure of testing a hypothesis, usually called a hypothesis test. Since this one has a test statistic involving z, it is also called a z-test. And since there is only one sample, it is usually called a one-sample z-test.

Example $\PageIndex{2}$ battery example revisited

State the random variable and the parameter in words.
State the null and alternative hypothesis and the level of significance.
A random sample of size n is taken.
The population standard derivation is known.
The sample size is at least 30 or the population of the random variable is normally distributed.
Find the sample statistic, test statistic, and p-value.
Interpretation

1. x = life of battery

$\mu$ = mean life of a XJ35 battery

2. $H_{o} : \mu=500$ days

$H_{A} : \mu<500$ days

$\alpha = 0.10$ (from above discussion about consequences)

3. Every hypothesis has some assumptions that be met to make sure that the results of the hypothesis are valid. The assumptions are different for each test. This test has the following assumptions.

This occurred in this example, since it was stated that a random sample of 30 battery lives were taken.
This is true, since it was given in the problem.
The sample size was 30, so this condition is met.

4. The test statistic depends on how many samples there are, what parameter you are testing, and assumptions that need to be checked. In this case, there is one sample and you are testing the mean. The assumptions were checked above.

Sample statistic:

$\overline{x} = 490$

Test statistic:

Using TI-83/84:

$P(\overline{x}<490 | \mu=500)=\text { normalcdf }(-1 \mathrm{E} 99,490,500,25 / \sqrt{30}) \approx 0.0142$

$P(\overline{x}<490 | \mu=500)=\operatorname{pnorm}(490,500,25 / \operatorname{sqrt}(30)) \approx 0.0142$

5. Now what? Well, this p-value is 0.0142. This is a lot smaller than the amount of error you would accept in the problem -$\alpha$ = 0.10. That means that finding a sample mean less than 490 days is unusual to happen if $H_{o}$ is true. This should make you think that $H_{o}$ is not true. You should reject $H_{o}$.

In fact, in general:

Reject $H_{o}$ if the p-value < $\alpha$ and

Fail to reject $H_{o}$ if the p-value $\geq \alpha$.

6. Since you rejected $H_{o}$, what does this mean in the real world? That is what goes in the interpretation. Since you rejected the claim by the manufacturer that the mean life of the batteries is 500 days, then you now can believe that your hypothesis was correct. In other words, there is enough evidence to show that the mean life of the battery is less than 500 days.

Now that you know that the batteries last less than 500 days, should you cancel the contract? Statistically, there is evidence that the batteries do not last as long as the manufacturer says they should. However, based on this sample there are only ten days less on average that the batteries last. There may not be practical significance in this case. Ten days do not seem like a large difference. In reality, if the batteries are used in pacemakers, then you would probably tell the patient to have the batteries replaced every year. You have a large buffer whether the batteries last 490 days or 500 days. It seems that it might not be worth it to break the contract over ten days. What if the 10 days was practically significant? Are there any other things you should consider? You might look at the business relationship with the manufacturer. You might also look at how much it would cost to find a new manufacturer. These are also questions to consider before making any changes. What this discussion should show you is that just because a hypothesis has statistical significance does not mean it has practical significance. The hypothesis test is just one part of a research process. There are other pieces that you need to consider.

That’s it. That is what a hypothesis test looks like. All hypothesis tests are done with the same six steps. Those general six steps are outlined below.

State the random variable and the parameter in words. This is where you are defining what the unknowns are in this problem. x = random variable $\mu$ = mean of random variable, if the parameter of interest is the mean. There are other parameters you can test, and you would use the appropriate symbol for that parameter.
State the null and alternative hypotheses and the level of significance $H_{o} : \mu=\mu_{o}$, where $\mu_{o}$ is the known mean $H_{A} : \mu<\mu_{o}$ $H_{A} : \mu>\mu_{o}$, use the appropriate one for your problem $H_{A} : \mu \neq \mu_{o}$ Also, state your $\alpha$ level here.
State and check the assumptions for a hypothesis test. Each hypothesis test has its own assumptions. They will be stated when the different hypothesis tests are discussed.
Find the sample statistic, test statistic, and p-value. This depends on what parameter you are working with, how many samples, and the assumptions of the test. The p-value depends on your $H_{A}$. If you are doing the $H_{A}$ with the less than, then it is a left-tailed test, and you find the probability of being in that left tail. If you are doing the $H_{A}$ with the greater than, then it is a right-tailed test, and you find the probability of being in the right tail. If you are doing the $H_{A}$ with the not equal to, then you are doing a two-tail test, and you find the probability of being in both tails. Because of symmetry, you could find the probability in one tail and double this value to find the probability in both tails.
Conclusion This is where you write reject $H_{o}$ or fail to reject $H_{o}$. The rule is: if the p-value < $\alpha$, then reject $H_{o}$. If the p-value $\geq \alpha$, then fail to reject $H_{o}$.
Interpretation This is where you interpret in real world terms the conclusion to the test. The conclusion for a hypothesis test is that you either have enough evidence to show $H_{A}$ is true, or you do not have enough evidence to show $H_{A}$ is true.

Sorry, one more concept about the conclusion and interpretation. First, the conclusion is that you reject $H_{o}$ or you fail to reject $H_{o}$. Why was it said like this? It is because you never accept the null hypothesis. If you wanted to accept the null hypothesis, then why do the test in the first place? In the interpretation, you either have enough evidence to show $H_{A}$ is true, or you do not have enough evidence to show $H_{A}$ is true. You wouldn’t want to go to all this work and then find out you wanted to accept the claim. Why go through the trouble? You always want to show that the alternative hypothesis is true. Sometimes you can do that and sometimes you can’t. It doesn’t mean you proved the null hypothesis; it just means you can’t prove the alternative hypothesis. Here is an example to demonstrate this.

Example $\PageIndex{3}$ conclusion in hypothesis tests

In the U.S. court system a jury trial could be set up as a hypothesis test. To really help you see how this works, let’s use OJ Simpson as an example. In the court system, a person is presumed innocent until he/she is proven guilty, and this is your null hypothesis. OJ Simpson was a football player in the 1970s. In 1994 his ex-wife and her friend were killed. OJ Simpson was accused of the crime, and in 1995 the case was tried. The prosecutors wanted to prove OJ was guilty of killing his wife and her friend, and that is the alternative hypothesis

$H_{0}$: OJ is innocent of killing his wife and her friend

$H_{A}$: OJ is guilty of killing his wife and her friend

In this case, a verdict of not guilty was given. That does not mean that he is innocent of this crime. It means there was not enough evidence to prove he was guilty. Many people believe that OJ was guilty of this crime, but the jury did not feel that the evidence presented was enough to show there was guilt. The verdict in a jury trial is always guilty or not guilty!

The same is true in a hypothesis test. There is either enough or not enough evidence to show that alternative hypothesis. It is not that you proved the null hypothesis true.

When identifying hypothesis, it is important to state your random variable and the appropriate parameter you want to make a decision about. If count something, then the random variable is the number of whatever you counted. The parameter is the proportion of what you counted. If the random variable is something you measured, then the parameter is the mean of what you measured. (Note: there are other parameters you can calculate, and some analysis of those will be presented in later chapters.)

Example $\PageIndex{4}$ stating hypotheses

Identify the hypotheses necessary to test the following statements:

The average salary of a teacher is more than $30,000.
The proportion of students who like math is less than 10%.
The average age of students in this class differs from 21.

a. x = salary of teacher

$\mu$ = mean salary of teacher

The guess is that $\mu>\$ 30,000$ and that is the alternative hypothesis.

The null hypothesis has the same parameter and number with an equal sign.

$\begin{array}{l}{H_{0} : \mu=\$ 30,000} \\ {H_{A} : \mu>\$ 30,000}\end{array}$

b. x = number od students who like math

p = proportion of students who like math

The guess is that p < 0.10 and that is the alternative hypothesis.

$\begin{array}{l}{H_{0} : p=0.10} \\ {H_{A} : p<0.10}\end{array}$

c. x = age of students in this class

$\mu$ = mean age of students in this class

The guess is that $\mu \neq 21$ and that is the alternative hypothesis.

$\begin{array}{c}{H_{0} : \mu=21} \\ {H_{A} : \mu \neq 21}\end{array}$

Example $\PageIndex{5}$ Stating Type I and II Errors and Picking Level of Significance

The plant-breeding department at a major university developed a new hybrid raspberry plant called YumYum Berry. Based on research data, the claim is made that from the time shoots are planted 90 days on average are required to obtain the first berry with a standard deviation of 9.2 days. A corporation that is interested in marketing the product tests 60 shoots by planting them and recording the number of days before each plant produces its first berry. The sample mean is 92.3 days. The corporation wants to know if the mean number of days is more than the 90 days claimed. State the type I and type II errors in terms of this problem, consequences of each error, and state which level of significance to use.
A concern was raised in Australia that the percentage of deaths of Aboriginal prisoners was higher than the percent of deaths of non-indigenous prisoners, which is 0.27%. State the type I and type II errors in terms of this problem, consequences of each error, and state which level of significance to use.

a. x = time to first berry for YumYum Berry plant

$\mu$ = mean time to first berry for YumYum Berry plant

$\begin{array}{l}{H_{0} : \mu=90} \\ {H_{A} : \mu>90}\end{array}$

Type I Error: If the corporation does a type I error, then they will say that the plants take longer to produce than 90 days when they don’t. They probably will not want to market the plants if they think they will take longer. They will not market them even though in reality the plants do produce in 90 days. They may have loss of future earnings, but that is all.

Type II error: The corporation do not say that the plants take longer then 90 days to produce when they do take longer. Most likely they will market the plants. The plants will take longer, and so customers might get upset and then the company would get a bad reputation. This would be really bad for the company.

Level of significance: It appears that the corporation would not want to make a type II error. Pick a 10% level of significance, $\alpha = 0.10$.

b. x = number of Aboriginal prisoners who have died

p = proportion of Aboriginal prisoners who have died

$\begin{array}{l}{H_{o} : p=0.27 \%} \\ {H_{A} : p>0.27 \%}\end{array}$

Type I error: Rejecting that the proportion of Aboriginal prisoners who died was 0.27%, when in fact it was 0.27%. This would mean you would say there is a problem when there isn’t one. You could anger the Aboriginal community, and spend time and energy researching something that isn’t a problem.

Type II error: Failing to reject that the proportion of Aboriginal prisoners who died was 0.27%, when in fact it is higher than 0.27%. This would mean that you wouldn’t think there was a problem with Aboriginal prisoners dying when there really is a problem. You risk causing deaths when there could be a way to avoid them.

Level of significance: It appears that both errors may be issues in this case. You wouldn’t want to anger the Aboriginal community when there isn’t an issue, and you wouldn’t want people to die when there may be a way to stop it. It may be best to pick a 5% level of significance, $\alpha = 0.05$.

Hypothesis testing is really easy if you follow the same recipe every time. The only differences in the various problems are the assumptions of the test and the test statistic you calculate so you can find the p-value. Do the same steps, in the same order, with the same words, every time and these problems become very easy.

Exercise $\PageIndex{1}$

For the problems in this section, a question is being asked. This is to help you understand what the hypotheses are. You are not to run any hypothesis tests and come up with any conclusions in this section.

Eyeglassomatic manufactures eyeglasses for different retailers. They test to see how many defective lenses they made in a given time period and found that 11% of all lenses had defects of some type. Looking at the type of defects, they found in a three-month time period that out of 34,641 defective lenses, 5865 were due to scratches. Are there more defects from scratches than from all other causes? State the random variable, population parameter, and hypotheses.
According to the February 2008 Federal Trade Commission report on consumer fraud and identity theft, 23% of all complaints in 2007 were for identity theft. In that year, Alaska had 321 complaints of identity theft out of 1,432 consumer complaints ("Consumer fraud and," 2008). Does this data provide enough evidence to show that Alaska had a lower proportion of identity theft than 23%? State the random variable, population parameter, and hypotheses.
The Kyoto Protocol was signed in 1997, and required countries to start reducing their carbon emissions. The protocol became enforceable in February 2005. In 2004, the mean CO2 emission was 4.87 metric tons per capita. Is there enough evidence to show that the mean CO2 emission is lower in 2010 than in 2004? State the random variable, population parameter, and hypotheses.
The FDA regulates that fish that is consumed is allowed to contain 1.0 mg/kg of mercury. In Florida, bass fish were collected in 53 different lakes to measure the amount of mercury in the fish. The data for the average amount of mercury in each lake is in Example $\PageIndex{5}$ ("Multi-disciplinary niser activity," 2013). Do the data provide enough evidence to show that the fish in Florida lakes has more mercury than the allowable amount? State the random variable, population parameter, and hypotheses.
Eyeglassomatic manufactures eyeglasses for different retailers. They test to see how many defective lenses they made in a given time period and found that 11% of all lenses had defects of some type. Looking at the type of defects, they found in a three-month time period that out of 34,641 defective lenses, 5865 were due to scratches. Are there more defects from scratches than from all other causes? State the type I and type II errors in this case, consequences of each error type for this situation from the perspective of the manufacturer, and the appropriate alpha level to use. State why you picked this alpha level.
According to the February 2008 Federal Trade Commission report on consumer fraud and identity theft, 23% of all complaints in 2007 were for identity theft. In that year, Alaska had 321 complaints of identity theft out of 1,432 consumer complaints ("Consumer fraud and," 2008). Does this data provide enough evidence to show that Alaska had a lower proportion of identity theft than 23%? State the type I and type II errors in this case, consequences of each error type for this situation from the perspective of the state of Arizona, and the appropriate alpha level to use. State why you picked this alpha level.
The Kyoto Protocol was signed in 1997, and required countries to start reducing their carbon emissions. The protocol became enforceable in February 2005. In 2004, the mean CO2 emission was 4.87 metric tons per capita. Is there enough evidence to show that the mean CO2 emission is lower in 2010 than in 2004? State the type I and type II errors in this case, consequences of each error type for this situation from the perspective of the agency overseeing the protocol, and the appropriate alpha level to use. State why you picked this alpha level.
The FDA regulates that fish that is consumed is allowed to contain 1.0 mg/kg of mercury. In Florida, bass fish were collected in 53 different lakes to measure the amount of mercury in the fish. The data for the average amount of mercury in each lake is in Example $\PageIndex{5}$ ("Multi-disciplinary niser activity," 2013). Do the data provide enough evidence to show that the fish in Florida lakes has more mercury than the allowable amount? State the type I and type II errors in this case, consequences of each error type for this situation from the perspective of the FDA, and the appropriate alpha level to use. State why you picked this alpha level.

1. $H_{o} : p=0.11, H_{A} : p>0.11$

3. $H_{o} : \mu=4.87 \text { metric tons per capita, } H_{A} : \mu<4.87 \text { metric tons per capita }$

5. See solutions

7. See solutions

Hypothesis Testing

Hypothesis testing is a tool for making statistical inferences about the population data. It is an analysis tool that tests assumptions and determines how likely something is within a given standard of accuracy. Hypothesis testing provides a way to verify whether the results of an experiment are valid.

A null hypothesis and an alternative hypothesis are set up before performing the hypothesis testing. This helps to arrive at a conclusion regarding the sample obtained from the population. In this article, we will learn more about hypothesis testing, its types, steps to perform the testing, and associated examples.

What is Hypothesis Testing in Statistics?

Hypothesis testing uses sample data from the population to draw useful conclusions regarding the population probability distribution . It tests an assumption made about the data using different types of hypothesis testing methodologies. The hypothesis testing results in either rejecting or not rejecting the null hypothesis.

Hypothesis Testing Definition

Hypothesis testing can be defined as a statistical tool that is used to identify if the results of an experiment are meaningful or not. It involves setting up a null hypothesis and an alternative hypothesis. These two hypotheses will always be mutually exclusive. This means that if the null hypothesis is true then the alternative hypothesis is false and vice versa. An example of hypothesis testing is setting up a test to check if a new medicine works on a disease in a more efficient manner.

Null Hypothesis

The null hypothesis is a concise mathematical statement that is used to indicate that there is no difference between two possibilities. In other words, there is no difference between certain characteristics of data. This hypothesis assumes that the outcomes of an experiment are based on chance alone. It is denoted as $H_{0}$. Hypothesis testing is used to conclude if the null hypothesis can be rejected or not. Suppose an experiment is conducted to check if girls are shorter than boys at the age of 5. The null hypothesis will say that they are the same height.

Alternative Hypothesis

The alternative hypothesis is an alternative to the null hypothesis. It is used to show that the observations of an experiment are due to some real effect. It indicates that there is a statistical significance between two possible outcomes and can be denoted as $H_{1}$ or $H_{a}$. For the above-mentioned example, the alternative hypothesis would be that girls are shorter than boys at the age of 5.

Hypothesis Testing P Value

In hypothesis testing, the p value is used to indicate whether the results obtained after conducting a test are statistically significant or not. It also indicates the probability of making an error in rejecting or not rejecting the null hypothesis.This value is always a number between 0 and 1. The p value is compared to an alpha level, $\alpha$ or significance level. The alpha level can be defined as the acceptable risk of incorrectly rejecting the null hypothesis. The alpha level is usually chosen between 1% to 5%.

Hypothesis Testing Critical region

All sets of values that lead to rejecting the null hypothesis lie in the critical region. Furthermore, the value that separates the critical region from the non-critical region is known as the critical value.

Hypothesis Testing Formula

Depending upon the type of data available and the size, different types of hypothesis testing are used to determine whether the null hypothesis can be rejected or not. The hypothesis testing formula for some important test statistics are given below:

z = $\frac{\overline{x}-\mu}{\frac{\sigma}{\sqrt{n}}}$. $\overline{x}$ is the sample mean, $\mu$ is the population mean, $\sigma$ is the population standard deviation and n is the size of the sample.
t = $\frac{\overline{x}-\mu}{\frac{s}{\sqrt{n}}}$. s is the sample standard deviation.
$\chi ^{2} = \sum \frac{(O_{i}-E_{i})^{2}}{E_{i}}$. $O_{i}$ is the observed value and $E_{i}$ is the expected value.

We will learn more about these test statistics in the upcoming section.

Types of Hypothesis Testing

Selecting the correct test for performing hypothesis testing can be confusing. These tests are used to determine a test statistic on the basis of which the null hypothesis can either be rejected or not rejected. Some of the important tests used for hypothesis testing are given below.

Hypothesis Testing Z Test

A z test is a way of hypothesis testing that is used for a large sample size (n ≥ 30). It is used to determine whether there is a difference between the population mean and the sample mean when the population standard deviation is known. It can also be used to compare the mean of two samples. It is used to compute the z test statistic. The formulas are given as follows:

One sample: z = $\frac{\overline{x}-\mu}{\frac{\sigma}{\sqrt{n}}}$.
Two samples: z = $\frac{(\overline{x_{1}}-\overline{x_{2}})-(\mu_{1}-\mu_{2})}{\sqrt{\frac{\sigma_{1}^{2}}{n_{1}}+\frac{\sigma_{2}^{2}}{n_{2}}}}$.

Hypothesis Testing t Test

The t test is another method of hypothesis testing that is used for a small sample size (n < 30). It is also used to compare the sample mean and population mean. However, the population standard deviation is not known. Instead, the sample standard deviation is known. The mean of two samples can also be compared using the t test.

One sample: t = $\frac{\overline{x}-\mu}{\frac{s}{\sqrt{n}}}$.
Two samples: t = $\frac{(\overline{x_{1}}-\overline{x_{2}})-(\mu_{1}-\mu_{2})}{\sqrt{\frac{s_{1}^{2}}{n_{1}}+\frac{s_{2}^{2}}{n_{2}}}}$.

Hypothesis Testing Chi Square

The Chi square test is a hypothesis testing method that is used to check whether the variables in a population are independent or not. It is used when the test statistic is chi-squared distributed.

One Tailed Hypothesis Testing

One tailed hypothesis testing is done when the rejection region is only in one direction. It can also be known as directional hypothesis testing because the effects can be tested in one direction only. This type of testing is further classified into the right tailed test and left tailed test.

Right Tailed Hypothesis Testing

The right tail test is also known as the upper tail test. This test is used to check whether the population parameter is greater than some value. The null and alternative hypotheses for this test are given as follows:

$H_{0}$: The population parameter is ≤ some value

$H_{1}$: The population parameter is > some value.

If the test statistic has a greater value than the critical value then the null hypothesis is rejected

Left Tailed Hypothesis Testing

The left tail test is also known as the lower tail test. It is used to check whether the population parameter is less than some value. The hypotheses for this hypothesis testing can be written as follows:

$H_{0}$: The population parameter is ≥ some value

$H_{1}$: The population parameter is < some value.

The null hypothesis is rejected if the test statistic has a value lesser than the critical value.

Two Tailed Hypothesis Testing

In this hypothesis testing method, the critical region lies on both sides of the sampling distribution. It is also known as a non - directional hypothesis testing method. The two-tailed test is used when it needs to be determined if the population parameter is assumed to be different than some value. The hypotheses can be set up as follows:

$H_{0}$: the population parameter = some value

$H_{1}$: the population parameter ≠ some value

The null hypothesis is rejected if the test statistic has a value that is not equal to the critical value.

Hypothesis Testing Steps

Hypothesis testing can be easily performed in five simple steps. The most important step is to correctly set up the hypotheses and identify the right method for hypothesis testing. The basic steps to perform hypothesis testing are as follows:

Step 1: Set up the null hypothesis by correctly identifying whether it is the left-tailed, right-tailed, or two-tailed hypothesis testing.
Step 2: Set up the alternative hypothesis.
Step 3: Choose the correct significance level, $\alpha$, and find the critical value.
Step 4: Calculate the correct test statistic (z, t or $\chi$) and p-value.
Step 5: Compare the test statistic with the critical value or compare the p-value with $\alpha$ to arrive at a conclusion. In other words, decide if the null hypothesis is to be rejected or not.

Hypothesis Testing Example

The best way to solve a problem on hypothesis testing is by applying the 5 steps mentioned in the previous section. Suppose a researcher claims that the mean average weight of men is greater than 100kgs with a standard deviation of 15kgs. 30 men are chosen with an average weight of 112.5 Kgs. Using hypothesis testing, check if there is enough evidence to support the researcher's claim. The confidence interval is given as 95%.

Step 1: This is an example of a right-tailed test. Set up the null hypothesis as $H_{0}$: $\mu$ = 100.

Step 2: The alternative hypothesis is given by $H_{1}$: $\mu$ > 100.

Step 3: As this is a one-tailed test, $\alpha$ = 100% - 95% = 5%. This can be used to determine the critical value.

1 - $\alpha$ = 1 - 0.05 = 0.95

0.95 gives the required area under the curve. Now using a normal distribution table, the area 0.95 is at z = 1.645. A similar process can be followed for a t-test. The only additional requirement is to calculate the degrees of freedom given by n - 1.

Step 4: Calculate the z test statistic. This is because the sample size is 30. Furthermore, the sample and population means are known along with the standard deviation.

z = $\frac{\overline{x}-\mu}{\frac{\sigma}{\sqrt{n}}}$.

$\mu$ = 100, $\overline{x}$ = 112.5, n = 30, $\sigma$ = 15

z = $\frac{112.5-100}{\frac{15}{\sqrt{30}}}$ = 4.56

Step 5: Conclusion. As 4.56 > 1.645 thus, the null hypothesis can be rejected.

Hypothesis Testing and Confidence Intervals

Confidence intervals form an important part of hypothesis testing. This is because the alpha level can be determined from a given confidence interval. Suppose a confidence interval is given as 95%. Subtract the confidence interval from 100%. This gives 100 - 95 = 5% or 0.05. This is the alpha value of a one-tailed hypothesis testing. To obtain the alpha value for a two-tailed hypothesis testing, divide this value by 2. This gives 0.05 / 2 = 0.025.

Probability and Statistics
Data Handling

Important Notes on Hypothesis Testing

Hypothesis testing is a technique that is used to verify whether the results of an experiment are statistically significant.
It involves the setting up of a null hypothesis and an alternate hypothesis.
There are three types of tests that can be conducted under hypothesis testing - z test, t test, and chi square test.
Hypothesis testing can be classified as right tail, left tail, and two tail tests.

Examples on Hypothesis Testing

Example 1: The average weight of a dumbbell in a gym is 90lbs. However, a physical trainer believes that the average weight might be higher. A random sample of 5 dumbbells with an average weight of 110lbs and a standard deviation of 18lbs. Using hypothesis testing check if the physical trainer's claim can be supported for a 95% confidence level. Solution: As the sample size is lesser than 30, the t-test is used. $H_{0}$: $\mu$ = 90, $H_{1}$: $\mu$ > 90 $\overline{x}$ = 110, $\mu$ = 90, n = 5, s = 18. $\alpha$ = 0.05 Using the t-distribution table, the critical value is 2.132 t = $\frac{\overline{x}-\mu}{\frac{s}{\sqrt{n}}}$ t = 2.484 As 2.484 > 2.132, the null hypothesis is rejected. Answer: The average weight of the dumbbells may be greater than 90lbs
Example 2: The average score on a test is 80 with a standard deviation of 10. With a new teaching curriculum introduced it is believed that this score will change. On random testing, the score of 38 students, the mean was found to be 88. With a 0.05 significance level, is there any evidence to support this claim? Solution: This is an example of two-tail hypothesis testing. The z test will be used. $H_{0}$: $\mu$ = 80, $H_{1}$: $\mu$ ≠ 80 $\overline{x}$ = 88, $\mu$ = 80, n = 36, $\sigma$ = 10. $\alpha$ = 0.05 / 2 = 0.025 The critical value using the normal distribution table is 1.96 z = $\frac{\overline{x}-\mu}{\frac{\sigma}{\sqrt{n}}}$ z = $\frac{88-80}{\frac{10}{\sqrt{36}}}$ = 4.8 As 4.8 > 1.96, the null hypothesis is rejected. Answer: There is a difference in the scores after the new curriculum was introduced.
Example 3: The average score of a class is 90. However, a teacher believes that the average score might be lower. The scores of 6 students were randomly measured. The mean was 82 with a standard deviation of 18. With a 0.05 significance level use hypothesis testing to check if this claim is true. Solution: The t test will be used. $H_{0}$: $\mu$ = 90, $H_{1}$: $\mu$ < 90 $\overline{x}$ = 110, $\mu$ = 90, n = 6, s = 18 The critical value from the t table is -2.015 t = $\frac{\overline{x}-\mu}{\frac{s}{\sqrt{n}}}$ t = $\frac{82-90}{\frac{18}{\sqrt{6}}}$ t = -1.088 As -1.088 > -2.015, we fail to reject the null hypothesis. Answer: There is not enough evidence to support the claim.

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FAQs on Hypothesis Testing

What is hypothesis testing.

Hypothesis testing in statistics is a tool that is used to make inferences about the population data. It is also used to check if the results of an experiment are valid.

What is the z Test in Hypothesis Testing?

The z test in hypothesis testing is used to find the z test statistic for normally distributed data . The z test is used when the standard deviation of the population is known and the sample size is greater than or equal to 30.

What is the t Test in Hypothesis Testing?

The t test in hypothesis testing is used when the data follows a student t distribution . It is used when the sample size is less than 30 and standard deviation of the population is not known.

What is the formula for z test in Hypothesis Testing?

The formula for a one sample z test in hypothesis testing is z = $\frac{\overline{x}-\mu}{\frac{\sigma}{\sqrt{n}}}$ and for two samples is z = $\frac{(\overline{x_{1}}-\overline{x_{2}})-(\mu_{1}-\mu_{2})}{\sqrt{\frac{\sigma_{1}^{2}}{n_{1}}+\frac{\sigma_{2}^{2}}{n_{2}}}}$.

What is the p Value in Hypothesis Testing?

The p value helps to determine if the test results are statistically significant or not. In hypothesis testing, the null hypothesis can either be rejected or not rejected based on the comparison between the p value and the alpha level.

What is One Tail Hypothesis Testing?

When the rejection region is only on one side of the distribution curve then it is known as one tail hypothesis testing. The right tail test and the left tail test are two types of directional hypothesis testing.

What is the Alpha Level in Two Tail Hypothesis Testing?

To get the alpha level in a two tail hypothesis testing divide $\alpha$ by 2. This is done as there are two rejection regions in the curve.

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17 Introduction to Hypothesis Testing

Jenna Lehmann

What is Hypothesis Testing?

Hypothesis testing is a big part of what we would actually consider testing for inferential statistics. It’s a procedure and set of rules that allow us to move from descriptive statistics to make inferences about a population based on sample data. It is a statistical method that uses sample data to evaluate a hypothesis about a population.

This type of test is usually used within the context of research. If we expect to see a difference between a treated and untreated group (in some cases the untreated group is the parameters we know about the population), we expect there to be a difference in the means between the two groups, but that the standard deviation remains the same, as if each individual score has had a value added or subtracted from it.

Steps of Hypothesis Testing

The following steps will be tailored to fit the first kind of hypothesis testing we will learn first: single-sample z-tests. There are many other kinds of tests, so keep this in mind.

Null Hypothesis (H0): states that in the general population there is no change, no difference, or no relationship, or in the context of an experiment, it predicts that the independent variable has no effect on the dependent variable.
Alternative Hypothesis (H1): states that there is a change, a difference, or a relationship for the general population, or in the context of an experiment, it predicts that the independent variable has an effect on the dependent variable.

$\alpha = 0.05,$

Critical Region: Composed of the extreme sample values that are very unlikely to be obtained if the null hypothesis is true. Determined by alpha level. If sample data fall in the critical region, the null hypothesis is rejected, because it’s very unlikely they’ve fallen there by chance.
After collecting the data, we find the sample mean. Now we can compare the sample mean with the null hypothesis by computing a z-score that describes where the sample mean is located relative to the hypothesized population mean. We use the z-score formula.
We decided previously what the two z-score boundaries are for a critical score. If the z-score we get after plugging the numbers in the aforementioned equation is outside of that critical region, we reject the null hypothesis. Otherwise, we would say that we failed to reject the null hypothesis.

Regions of the Distribution

Because we’re making judgments based on probability and proportion, our normal distributions and certain regions within them come into play.

The Critical Region is composed of the extreme sample values that are very unlikely to be obtained if the null hypothesis is true. Determined by alpha level. If sample data fall in the critical region, the null hypothesis is rejected, because it’s very unlikely they’ve fallen there by chance.

These regions come into play when talking about different errors.

A Type I Error occurs when a researcher rejects a null hypothesis that is actually true; the researcher concludes that a treatment has an effect when it actually doesn’t. This happens when a researcher unknowingly obtains an extreme, non-representative sample. This goes back to alpha level: it’s the probability that the test will lead to a Type I error if the null hypothesis is true.

$(\beta)$

A result is said to be significant or statistically significant if it is very unlikely to occur when the null hypothesis is true. That is, the result is sufficient to reject the null hypothesis. For instance, two means can be significantly different from one another.

Factors that Influence and Assumptions of Hypothesis Testing

Assumptions of Hypothesis Testing:

Random sampling: it is assumed that the participants used in the study were selected randomly so that we can confidently generalize our findings from the sample to the population.
Independent observation: two observations are independent if there is no consistent, predictable relationship between the first observation and the second. The value of σ is unchanged by the treatment; if the population standard deviation is unknown, we assume that the standard deviation for the unknown population (after treatment) is the same as it was for the population before treatment. There are ways of checking to see if this is true in SPSS or Excel.
Normal sampling distribution: in order to use the unit normal table to identify the critical region, we need the distribution of sample means to be normal (which means we need the population to be distributed normally and/or each sample size needs to be 30 or greater based on what we know about the central limit theorem).

Factors that influence hypothesis testing:

The variability of the scores, which is measured by either the standard deviation or the variance. The variability influences the size of the standard error in the denominator of the z-score.
The number of scores in the sample. This value also influences the size of the standard error in the denominator.

Test statistic: indicates that the sample data are converted into a single, specific statistic that is used to test the hypothesis (in this case, the z-score statistic).

Directional Hypotheses and Tailed Tests

In a directional hypothesis test , also known as a one-tailed test, the statistical hypotheses specify with an increase or decrease in the population mean. That is, they make a statement about the direction of the effect.

The Hypotheses for a Directional Test:

H0: The test scores are not increased/decreased (the treatment doesn’t work)
H1: The test scores are increased/decreased (the treatment works as predicted)

Because we’re only worried about scores that are either greater or less than the scores predicted by the null hypothesis, we only worry about what’s going on in one tail meaning that the critical region only exists within one tail. This means that all of the alpha is contained in one tail rather than split up into both (so the whole 5% is located in the tail we care about, rather than 2.5% in each tail). So before, we cared about what’s going on at the 0.025 mark of the unit normal table to look at both tails, but now we care about 0.05 because we’re only looking at one tail.

A one-tailed test allows you to reject the null hypothesis when the difference between the sample and the population is relatively small, as long as that difference is in the direction that you predicted. A two-tailed test, on the other hand, requires a relatively large difference independent of direction. In practice, researchers hypothesize using a one-tailed method but base their findings off of whether the results fall into the critical region of a two-tailed method. For the purposes of this class, make sure to calculate your results using the test that is specified in the problem.

Effect Size

A measure of effect size is intended to provide a measurement of the absolute magnitude of a treatment effect, independent of the size of the sample(s) being used. Usually done with Cohen’s d. If you imagine the two distributions, they’re layered over one another. The more they overlap, the smaller the effect size (the means of the two distributions are close). The more they are spread apart, the greater the effect size (the means of the two distributions are farther apart).

Statistical Power

The power of a statistical test is the probability that the test will correctly reject a false null hypothesis. It’s usually what we’re hoping to get when we run an experiment. It’s displayed in the table posted above. Power and effect size are connected. So, we know that the greater the distance between the means, the greater the effect size. If the two distributions overlapped very little, there would be a greater chance of selecting a sample that leads to rejecting the null hypothesis.

This chapter was originally posted to the Math Support Center blog at the University of Baltimore on June 11, 2019.

Math and Statistics Guides from UB's Math & Statistics Center Copyright © by Jenna Lehmann is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License , except where otherwise noted.

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Statistics Made Easy

Introduction to Hypothesis Testing

A statistical hypothesis is an assumption about a population parameter .

For example, we may assume that the mean height of a male in the U.S. is 70 inches.

The assumption about the height is the statistical hypothesis and the true mean height of a male in the U.S. is the population parameter .

A hypothesis test is a formal statistical test we use to reject or fail to reject a statistical hypothesis.

The Two Types of Statistical Hypotheses

To test whether a statistical hypothesis about a population parameter is true, we obtain a random sample from the population and perform a hypothesis test on the sample data.

There are two types of statistical hypotheses:

The null hypothesis , denoted as H 0 , is the hypothesis that the sample data occurs purely from chance.

The alternative hypothesis , denoted as H 1 or H a , is the hypothesis that the sample data is influenced by some non-random cause.

Hypothesis Tests

A hypothesis test consists of five steps:

1. State the hypotheses.

State the null and alternative hypotheses. These two hypotheses need to be mutually exclusive, so if one is true then the other must be false.

2. Determine a significance level to use for the hypothesis.

Decide on a significance level. Common choices are .01, .05, and .1.

3. Find the test statistic.

Find the test statistic and the corresponding p-value. Often we are analyzing a population mean or proportion and the general formula to find the test statistic is: (sample statistic – population parameter) / (standard deviation of statistic)

4. Reject or fail to reject the null hypothesis.

Using the test statistic or the p-value, determine if you can reject or fail to reject the null hypothesis based on the significance level.

The p-value tells us the strength of evidence in support of a null hypothesis. If the p-value is less than the significance level, we reject the null hypothesis.

5. Interpret the results.

Interpret the results of the hypothesis test in the context of the question being asked.

The Two Types of Decision Errors

There are two types of decision errors that one can make when doing a hypothesis test:

Type I error: You reject the null hypothesis when it is actually true. The probability of committing a Type I error is equal to the significance level, often called alpha , and denoted as α.

Type II error: You fail to reject the null hypothesis when it is actually false. The probability of committing a Type II error is called the Power of the test or Beta , denoted as β.

One-Tailed and Two-Tailed Tests

A statistical hypothesis can be one-tailed or two-tailed.

A one-tailed hypothesis involves making a “greater than” or “less than ” statement.

For example, suppose we assume the mean height of a male in the U.S. is greater than or equal to 70 inches. The null hypothesis would be H0: µ ≥ 70 inches and the alternative hypothesis would be Ha: µ < 70 inches.

A two-tailed hypothesis involves making an “equal to” or “not equal to” statement.

For example, suppose we assume the mean height of a male in the U.S. is equal to 70 inches. The null hypothesis would be H0: µ = 70 inches and the alternative hypothesis would be Ha: µ ≠ 70 inches.

Note: The “equal” sign is always included in the null hypothesis, whether it is =, ≥, or ≤.

Related: What is a Directional Hypothesis?

Types of Hypothesis Tests

There are many different types of hypothesis tests you can perform depending on the type of data you’re working with and the goal of your analysis.

The following tutorials provide an explanation of the most common types of hypothesis tests:

Introduction to the One Sample t-test Introduction to the Two Sample t-test Introduction to the Paired Samples t-test Introduction to the One Proportion Z-Test Introduction to the Two Proportion Z-Test

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Hypothesis Testing

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A hypothesis test is a statistical inference method used to test the significance of a proposed (hypothesized) relation between population statistics (parameters) and their corresponding sample estimators . In other words, hypothesis tests are used to determine if there is enough evidence in a sample to prove a hypothesis true for the entire population.

The test considers two hypotheses: the null hypothesis , which is a statement meant to be tested, usually something like "there is no effect" with the intention of proving this false, and the alternate hypothesis , which is the statement meant to stand after the test is performed. The two hypotheses must be mutually exclusive ; moreover, in most applications, the two are complementary (one being the negation of the other). The test works by comparing the $p$-value to the level of significance (a chosen target). If the $p$-value is less than or equal to the level of significance, then the null hypothesis is rejected.

When analyzing data, only samples of a certain size might be manageable as efficient computations. In some situations the error terms follow a continuous or infinite distribution, hence the use of samples to suggest accuracy of the chosen test statistics. The method of hypothesis testing gives an advantage over guessing what distribution or which parameters the data follows.

Definitions and Methodology

Hypothesis test and confidence intervals.

In statistical inference, properties (parameters) of a population are analyzed by sampling data sets. Given assumptions on the distribution, i.e. a statistical model of the data, certain hypotheses can be deduced from the known behavior of the model. These hypotheses must be tested against sampled data from the population.

The null hypothesis $($denoted $H_0)$ is a statement that is assumed to be true. If the null hypothesis is rejected, then there is enough evidence (statistical significance) to accept the alternate hypothesis $($denoted $H_1).$ Before doing any test for significance, both hypotheses must be clearly stated and non-conflictive, i.e. mutually exclusive, statements. Rejecting the null hypothesis, given that it is true, is called a type I error and it is denoted $\alpha$, which is also its probability of occurrence. Failing to reject the null hypothesis, given that it is false, is called a type II error and it is denoted $\beta$, which is also its probability of occurrence. Also, $\alpha$ is known as the significance level , and $1-\beta$ is known as the power of the test. $H_0$ $\textbf{is true}$$\hspace{15mm}$ $H_0$ $\textbf{is false}$ $\textbf{Reject}$ $H_0$$\hspace{10mm}$ Type I error Correct Decision $\textbf{Reject}$ $H_1$ Correct Decision Type II error The test statistic is the standardized value following the sampled data under the assumption that the null hypothesis is true, and a chosen particular test. These tests depend on the statistic to be studied and the assumed distribution it follows, e.g. the population mean following a normal distribution. The $p$-value is the probability of observing an extreme test statistic in the direction of the alternate hypothesis, given that the null hypothesis is true. The critical value is the value of the assumed distribution of the test statistic such that the probability of making a type I error is small.

Methodologies: Given an estimator $\hat \theta$ of a population statistic $\theta$, following a probability distribution $P(T)$, computed from a sample $\mathcal{S},$ and given a significance level $\alpha$ and test statistic $t^*,$ define $H_0$ and $H_1;$ compute the test statistic $t^*.$ $p$-value Approach (most prevalent): Find the $p$-value using $t^*$ (right-tailed). If the $p$-value is at most $\alpha,$ reject $H_0$. Otherwise, reject $H_1$. Critical Value Approach: Find the critical value solving the equation $P(T\geq t_\alpha)=\alpha$ (right-tailed). If $t^*>t_\alpha$, reject $H_0$. Otherwise, reject $H_1$. Note: Failing to reject $H_0$ only means inability to accept $H_1$, and it does not mean to accept $H_0$.

Assume a normally distributed population has recorded cholesterol levels with various statistics computed. From a sample of 100 subjects in the population, the sample mean was 214.12 mg/dL (milligrams per deciliter), with a sample standard deviation of 45.71 mg/dL. Perform a hypothesis test, with significance level 0.05, to test if there is enough evidence to conclude that the population mean is larger than 200 mg/dL. Hypothesis Test We will perform a hypothesis test using the $p$-value approach with significance level $\alpha=0.05:$ Define $H_0$: $\mu=200$. Define $H_1$: $\mu>200$. Since our values are normally distributed, the test statistic is $z^*=\frac{\bar X - \mu_0}{\frac{s}{\sqrt{n}}}=\frac{214.12 - 200}{\frac{45.71}{\sqrt{100}}}\approx 3.09$. Using a standard normal distribution, we find that our $p$-value is approximately $0.001$. Since the $p$-value is at most $\alpha=0.05,$ we reject $H_0$. Therefore, we can conclude that the test shows sufficient evidence to support the claim that $\mu$ is larger than $200$ mg/dL.

If the sample size was smaller, the normal and $t$-distributions behave differently. Also, the question itself must be managed by a double-tail test instead.

Assume a population's cholesterol levels are recorded and various statistics are computed. From a sample of 25 subjects, the sample mean was 214.12 mg/dL (milligrams per deciliter), with a sample standard deviation of 45.71 mg/dL. Perform a hypothesis test, with significance level 0.05, to test if there is enough evidence to conclude that the population mean is not equal to 200 mg/dL. Hypothesis Test We will perform a hypothesis test using the $p$-value approach with significance level $\alpha=0.05$ and the $t$-distribution with 24 degrees of freedom: Define $H_0$: $\mu=200$. Define $H_1$: $\mu\neq 200$. Using the $t$-distribution, the test statistic is $t^*=\frac{\bar X - \mu_0}{\frac{s}{\sqrt{n}}}=\frac{214.12 - 200}{\frac{45.71}{\sqrt{25}}}\approx 1.54$. Using a $t$-distribution with 24 degrees of freedom, we find that our $p$-value is approximately $2(0.068)=0.136$. We have multiplied by two since this is a two-tailed argument, i.e. the mean can be smaller than or larger than. Since the $p$-value is larger than $\alpha=0.05,$ we fail to reject $H_0$. Therefore, the test does not show sufficient evidence to support the claim that $\mu$ is not equal to $200$ mg/dL.

The complement of the rejection on a two-tailed hypothesis test (with significance level $\alpha$) for a population parameter $\theta$ is equivalent to finding a confidence interval $($with confidence level $1-\alpha)$ for the population parameter $\theta$. If the assumption on the parameter $\theta$ falls inside the confidence interval, then the test has failed to reject the null hypothesis $($with $p$-value greater than $\alpha).$ Otherwise, if $\theta$ does not fall in the confidence interval, then the null hypothesis is rejected in favor of the alternate $($with $p$-value at most $\alpha).$

Statistics (Estimation)
Normal Distribution
Correlation
Confidence Intervals

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Unit 12: Significance tests (hypothesis testing)

About this unit, the idea of significance tests.

Simple hypothesis testing (Opens a modal)
Idea behind hypothesis testing (Opens a modal)
Examples of null and alternative hypotheses (Opens a modal)
P-values and significance tests (Opens a modal)
Comparing P-values to different significance levels (Opens a modal)
Estimating a P-value from a simulation (Opens a modal)
Using P-values to make conclusions (Opens a modal)
Simple hypothesis testing Get 3 of 4 questions to level up!
Writing null and alternative hypotheses Get 3 of 4 questions to level up!
Estimating P-values from simulations Get 3 of 4 questions to level up!

Error probabilities and power

Introduction to Type I and Type II errors (Opens a modal)
Type 1 errors (Opens a modal)
Examples identifying Type I and Type II errors (Opens a modal)
Introduction to power in significance tests (Opens a modal)
Examples thinking about power in significance tests (Opens a modal)
Consequences of errors and significance (Opens a modal)
Type I vs Type II error Get 3 of 4 questions to level up!
Error probabilities and power Get 3 of 4 questions to level up!

Tests about a population proportion

Constructing hypotheses for a significance test about a proportion (Opens a modal)
Conditions for a z test about a proportion (Opens a modal)
Reference: Conditions for inference on a proportion (Opens a modal)
Calculating a z statistic in a test about a proportion (Opens a modal)
Calculating a P-value given a z statistic (Opens a modal)
Making conclusions in a test about a proportion (Opens a modal)
Writing hypotheses for a test about a proportion Get 3 of 4 questions to level up!
Conditions for a z test about a proportion Get 3 of 4 questions to level up!
Calculating the test statistic in a z test for a proportion Get 3 of 4 questions to level up!
Calculating the P-value in a z test for a proportion Get 3 of 4 questions to level up!
Making conclusions in a z test for a proportion Get 3 of 4 questions to level up!

Tests about a population mean

Writing hypotheses for a significance test about a mean (Opens a modal)
Conditions for a t test about a mean (Opens a modal)
Reference: Conditions for inference on a mean (Opens a modal)
When to use z or t statistics in significance tests (Opens a modal)
Example calculating t statistic for a test about a mean (Opens a modal)
Using TI calculator for P-value from t statistic (Opens a modal)
Using a table to estimate P-value from t statistic (Opens a modal)
Comparing P-value from t statistic to significance level (Opens a modal)
Free response example: Significance test for a mean (Opens a modal)
Writing hypotheses for a test about a mean Get 3 of 4 questions to level up!
Conditions for a t test about a mean Get 3 of 4 questions to level up!
Calculating the test statistic in a t test for a mean Get 3 of 4 questions to level up!
Calculating the P-value in a t test for a mean Get 3 of 4 questions to level up!
Making conclusions in a t test for a mean Get 3 of 4 questions to level up!

What is a Hypothesis?

A hypothesis is a calculated prediction or assumption about a population parameter based on limited evidence. The whole idea behind hypothesis formulation is testing—this means the researcher subjects his or her calculated assumption to a series of evaluations to know whether they are true or false.

Typically, every research starts with a hypothesis—the investigator makes a claim and experiments to prove that this claim is true or false . For instance, if you predict that students who drink milk before class perform better than those who don’t, then this becomes a hypothesis that can be confirmed or refuted using an experiment.

Read: What is Empirical Research Study? [Examples & Method]

What are the Types of Hypotheses?

1. simple hypothesis.

Also known as a basic hypothesis, a simple hypothesis suggests that an independent variable is responsible for a corresponding dependent variable. In other words, an occurrence of the independent variable inevitably leads to an occurrence of the dependent variable.

Typically, simple hypotheses are considered as generally true, and they establish a causal relationship between two variables.

Examples of Simple Hypothesis

Drinking soda and other sugary drinks can cause obesity.
Smoking cigarettes daily leads to lung cancer.

2. Complex Hypothesis

A complex hypothesis is also known as a modal. It accounts for the causal relationship between two independent variables and the resulting dependent variables. This means that the combination of the independent variables leads to the occurrence of the dependent variables .

Examples of Complex Hypotheses

Adults who do not smoke and drink are less likely to develop liver-related conditions.
Global warming causes icebergs to melt which in turn causes major changes in weather patterns.

3. Null Hypothesis

As the name suggests, a null hypothesis is formed when a researcher suspects that there’s no relationship between the variables in an observation. In this case, the purpose of the research is to approve or disapprove this assumption.

Examples of Null Hypothesis

This is no significant change in a student’s performance if they drink coffee or tea before classes.
There’s no significant change in the growth of a plant if one uses distilled water only or vitamin-rich water.

Read: Research Report: Definition, Types + [Writing Guide]

4. Alternative Hypothesis

To disapprove a null hypothesis, the researcher has to come up with an opposite assumption—this assumption is known as the alternative hypothesis. This means if the null hypothesis says that A is false, the alternative hypothesis assumes that A is true.

An alternative hypothesis can be directional or non-directional depending on the direction of the difference. A directional alternative hypothesis specifies the direction of the tested relationship, stating that one variable is predicted to be larger or smaller than the null value while a non-directional hypothesis only validates the existence of a difference without stating its direction.

Examples of Alternative Hypotheses

Starting your day with a cup of tea instead of a cup of coffee can make you more alert in the morning.
The growth of a plant improves significantly when it receives distilled water instead of vitamin-rich water.

5. Logical Hypothesis

Logical hypotheses are some of the most common types of calculated assumptions in systematic investigations. It is an attempt to use your reasoning to connect different pieces in research and build a theory using little evidence. In this case, the researcher uses any data available to him, to form a plausible assumption that can be tested.

Examples of Logical Hypothesis

Waking up early helps you to have a more productive day.
Beings from Mars would not be able to breathe the air in the atmosphere of the Earth.

6. Empirical Hypothesis

After forming a logical hypothesis, the next step is to create an empirical or working hypothesis. At this stage, your logical hypothesis undergoes systematic testing to prove or disprove the assumption. An empirical hypothesis is subject to several variables that can trigger changes and lead to specific outcomes.

Examples of Empirical Testing

People who eat more fish run faster than people who eat meat.
Women taking vitamin E grow hair faster than those taking vitamin K.

7. Statistical Hypothesis

When forming a statistical hypothesis, the researcher examines the portion of a population of interest and makes a calculated assumption based on the data from this sample. A statistical hypothesis is most common with systematic investigations involving a large target audience. Here, it’s impossible to collect responses from every member of the population so you have to depend on data from your sample and extrapolate the results to the wider population.

Examples of Statistical Hypothesis

45% of students in Louisiana have middle-income parents.
80% of the UK’s population gets a divorce because of irreconcilable differences.

What is Hypothesis Testing?

Hypothesis testing is an assessment method that allows researchers to determine the plausibility of a hypothesis. It involves testing an assumption about a specific population parameter to know whether it’s true or false. These population parameters include variance, standard deviation, and median.

Typically, hypothesis testing starts with developing a null hypothesis and then performing several tests that support or reject the null hypothesis. The researcher uses test statistics to compare the association or relationship between two or more variables.

Explore: Research Bias: Definition, Types + Examples

Researchers also use hypothesis testing to calculate the coefficient of variation and determine if the regression relationship and the correlation coefficient are statistically significant.

How Hypothesis Testing Works

The basis of hypothesis testing is to examine and analyze the null hypothesis and alternative hypothesis to know which one is the most plausible assumption. Since both assumptions are mutually exclusive, only one can be true. In other words, the occurrence of a null hypothesis destroys the chances of the alternative coming to life, and vice-versa.

Interesting: 21 Chrome Extensions for Academic Researchers in 2021

What Are The Stages of Hypothesis Testing?

To successfully confirm or refute an assumption, the researcher goes through five (5) stages of hypothesis testing;

Determine the null hypothesis
Specify the alternative hypothesis
Set the significance level
Calculate the test statistics and corresponding P-value
Draw your conclusion
Determine the Null Hypothesis

Like we mentioned earlier, hypothesis testing starts with creating a null hypothesis which stands as an assumption that a certain statement is false or implausible. For example, the null hypothesis (H0) could suggest that different subgroups in the research population react to a variable in the same way.

Specify the Alternative Hypothesis

Once you know the variables for the null hypothesis, the next step is to determine the alternative hypothesis. The alternative hypothesis counters the null assumption by suggesting the statement or assertion is true. Depending on the purpose of your research, the alternative hypothesis can be one-sided or two-sided.

Using the example we established earlier, the alternative hypothesis may argue that the different sub-groups react differently to the same variable based on several internal and external factors.

Set the Significance Level

Many researchers create a 5% allowance for accepting the value of an alternative hypothesis, even if the value is untrue. This means that there is a 0.05 chance that one would go with the value of the alternative hypothesis, despite the truth of the null hypothesis.

Something to note here is that the smaller the significance level, the greater the burden of proof needed to reject the null hypothesis and support the alternative hypothesis.

Explore: What is Data Interpretation? + [Types, Method & Tools]

Calculate the Test Statistics and Corresponding P-Value

Test statistics in hypothesis testing allow you to compare different groups between variables while the p-value accounts for the probability of obtaining sample statistics if your null hypothesis is true. In this case, your test statistics can be the mean, median and similar parameters.

If your p-value is 0.65, for example, then it means that the variable in your hypothesis will happen 65 in100 times by pure chance. Use this formula to determine the p-value for your data:

Draw Your Conclusions

After conducting a series of tests, you should be able to agree or refute the hypothesis based on feedback and insights from your sample data.

Applications of Hypothesis Testing in Research

Hypothesis testing isn’t only confined to numbers and calculations; it also has several real-life applications in business, manufacturing, advertising, and medicine.

In a factory or other manufacturing plants, hypothesis testing is an important part of quality and production control before the final products are approved and sent out to the consumer.

During ideation and strategy development, C-level executives use hypothesis testing to evaluate their theories and assumptions before any form of implementation. For example, they could leverage hypothesis testing to determine whether or not some new advertising campaign, marketing technique, etc. causes increased sales.

In addition, hypothesis testing is used during clinical trials to prove the efficacy of a drug or new medical method before its approval for widespread human usage.

What is an Example of Hypothesis Testing?

An employer claims that her workers are of above-average intelligence. She takes a random sample of 20 of them and gets the following results:

Mean IQ Scores: 110

Standard Deviation: 15

Mean Population IQ: 100

Step 1: Using the value of the mean population IQ, we establish the null hypothesis as 100.

Step 2: State that the alternative hypothesis is greater than 100.

Step 3: State the alpha level as 0.05 or 5%

Step 4: Find the rejection region area (given by your alpha level above) from the z-table. An area of .05 is equal to a z-score of 1.645.

Step 5: Calculate the test statistics using this formula

Z = (110–100) ÷ (15÷√20)

10 ÷ 3.35 = 2.99

If the value of the test statistics is higher than the value of the rejection region, then you should reject the null hypothesis. If it is less, then you cannot reject the null.

In this case, 2.99 > 1.645 so we reject the null.

Importance/Benefits of Hypothesis Testing

The most significant benefit of hypothesis testing is it allows you to evaluate the strength of your claim or assumption before implementing it in your data set. Also, hypothesis testing is the only valid method to prove that something “is or is not”. Other benefits include:

Hypothesis testing provides a reliable framework for making any data decisions for your population of interest.
It helps the researcher to successfully extrapolate data from the sample to the larger population.
Hypothesis testing allows the researcher to determine whether the data from the sample is statistically significant.
Hypothesis testing is one of the most important processes for measuring the validity and reliability of outcomes in any systematic investigation.
It helps to provide links to the underlying theory and specific research questions.

Criticism and Limitations of Hypothesis Testing

Several limitations of hypothesis testing can affect the quality of data you get from this process. Some of these limitations include:

The interpretation of a p-value for observation depends on the stopping rule and definition of multiple comparisons. This makes it difficult to calculate since the stopping rule is subject to numerous interpretations, plus “multiple comparisons” are unavoidably ambiguous.
Conceptual issues often arise in hypothesis testing, especially if the researcher merges Fisher and Neyman-Pearson’s methods which are conceptually distinct.
In an attempt to focus on the statistical significance of the data, the researcher might ignore the estimation and confirmation by repeated experiments.
Hypothesis testing can trigger publication bias, especially when it requires statistical significance as a criterion for publication.
When used to detect whether a difference exists between groups, hypothesis testing can trigger absurd assumptions that affect the reliability of your observation.

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Type I vs Type II Errors: Causes, Examples & Prevention

This article will discuss the two different types of errors in hypothesis testing and how you can prevent them from occurring in your research

Internal Validity in Research: Definition, Threats, Examples

In this article, we will discuss the concept of internal validity, some clear examples, its importance, and how to test it.

What is Pure or Basic Research? + [Examples & Method]

Simple guide on pure or basic research, its methods, characteristics, advantages, and examples in science, medicine, education and psychology

Alternative vs Null Hypothesis: Pros, Cons, Uses & Examples

We are going to discuss alternative hypotheses and null hypotheses in this post and how they work in research.

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Tutorial Playlist

Statistics tutorial, everything you need to know about the probability density function in statistics, the best guide to understand central limit theorem, an in-depth guide to measures of central tendency : mean, median and mode, the ultimate guide to understand conditional probability.

A Comprehensive Look at Percentile in Statistics

The Best Guide to Understand Bayes Theorem

Everything you need to know about the normal distribution, an in-depth explanation of cumulative distribution function, a complete guide to chi-square test, a complete guide on hypothesis testing in statistics, understanding the fundamentals of arithmetic and geometric progression, the definitive guide to understand spearman’s rank correlation, a comprehensive guide to understand mean squared error, all you need to know about the empirical rule in statistics, the complete guide to skewness and kurtosis, a holistic look at bernoulli distribution.

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The Complete Guide to Understand Pearson's Correlation

A complete guide on the types of statistical studies, everything you need to know about poisson distribution, your best guide to understand correlation vs. regression, the most comprehensive guide for beginners on what is correlation, what is hypothesis testing in statistics types and examples.

Lesson 10 of 24 By Avijeet Biswal

A Complete Guide on Hypothesis Testing in Statistics

In today’s data-driven world , decisions are based on data all the time. Hypothesis plays a crucial role in that process, whether it may be making business decisions, in the health sector, academia, or in quality improvement. Without hypothesis & hypothesis tests, you risk drawing the wrong conclusions and making bad decisions. In this tutorial, you will look at Hypothesis Testing in Statistics.

What Is Hypothesis Testing in Statistics?

Hypothesis Testing is a type of statistical analysis in which you put your assumptions about a population parameter to the test. It is used to estimate the relationship between 2 statistical variables.

Let's discuss few examples of statistical hypothesis from real-life -

A teacher assumes that 60% of his college's students come from lower-middle-class families.
A doctor believes that 3D (Diet, Dose, and Discipline) is 90% effective for diabetic patients.

Now that you know about hypothesis testing, look at the two types of hypothesis testing in statistics.

Hypothesis Testing Formula

Z = ( x̅ – μ0 ) / (σ /√n)

Here, x̅ is the sample mean,
μ0 is the population mean,
σ is the standard deviation,
n is the sample size.

How Hypothesis Testing Works?

An analyst performs hypothesis testing on a statistical sample to present evidence of the plausibility of the null hypothesis. Measurements and analyses are conducted on a random sample of the population to test a theory. Analysts use a random population sample to test two hypotheses: the null and alternative hypotheses.

The null hypothesis is typically an equality hypothesis between population parameters; for example, a null hypothesis may claim that the population means return equals zero. The alternate hypothesis is essentially the inverse of the null hypothesis (e.g., the population means the return is not equal to zero). As a result, they are mutually exclusive, and only one can be correct. One of the two possibilities, however, will always be correct.

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Null Hypothesis and Alternate Hypothesis

The Null Hypothesis is the assumption that the event will not occur. A null hypothesis has no bearing on the study's outcome unless it is rejected.

H0 is the symbol for it, and it is pronounced H-naught.

The Alternate Hypothesis is the logical opposite of the null hypothesis. The acceptance of the alternative hypothesis follows the rejection of the null hypothesis. H1 is the symbol for it.

Let's understand this with an example.

A sanitizer manufacturer claims that its product kills 95 percent of germs on average.

To put this company's claim to the test, create a null and alternate hypothesis.

H0 (Null Hypothesis): Average = 95%.

Alternative Hypothesis (H1): The average is less than 95%.

Another straightforward example to understand this concept is determining whether or not a coin is fair and balanced. The null hypothesis states that the probability of a show of heads is equal to the likelihood of a show of tails. In contrast, the alternate theory states that the probability of a show of heads and tails would be very different.

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Hypothesis Testing Calculation With Examples

Let's consider a hypothesis test for the average height of women in the United States. Suppose our null hypothesis is that the average height is 5'4". We gather a sample of 100 women and determine that their average height is 5'5". The standard deviation of population is 2.

To calculate the z-score, we would use the following formula:

z = ( x̅ – μ0 ) / (σ /√n)

z = (5'5" - 5'4") / (2" / √100)

z = 0.5 / (0.045)

We will reject the null hypothesis as the z-score of 11.11 is very large and conclude that there is evidence to suggest that the average height of women in the US is greater than 5'4".

Steps of Hypothesis Testing

Step 1: specify your null and alternate hypotheses.

It is critical to rephrase your original research hypothesis (the prediction that you wish to study) as a null (Ho) and alternative (Ha) hypothesis so that you can test it quantitatively. Your first hypothesis, which predicts a link between variables, is generally your alternate hypothesis. The null hypothesis predicts no link between the variables of interest.

Step 2: Gather Data

For a statistical test to be legitimate, sampling and data collection must be done in a way that is meant to test your hypothesis. You cannot draw statistical conclusions about the population you are interested in if your data is not representative.

Step 3: Conduct a Statistical Test

Other statistical tests are available, but they all compare within-group variance (how to spread out the data inside a category) against between-group variance (how different the categories are from one another). If the between-group variation is big enough that there is little or no overlap between groups, your statistical test will display a low p-value to represent this. This suggests that the disparities between these groups are unlikely to have occurred by accident. Alternatively, if there is a large within-group variance and a low between-group variance, your statistical test will show a high p-value. Any difference you find across groups is most likely attributable to chance. The variety of variables and the level of measurement of your obtained data will influence your statistical test selection.

Step 4: Determine Rejection Of Your Null Hypothesis

Your statistical test results must determine whether your null hypothesis should be rejected or not. In most circumstances, you will base your judgment on the p-value provided by the statistical test. In most circumstances, your preset level of significance for rejecting the null hypothesis will be 0.05 - that is, when there is less than a 5% likelihood that these data would be seen if the null hypothesis were true. In other circumstances, researchers use a lower level of significance, such as 0.01 (1%). This reduces the possibility of wrongly rejecting the null hypothesis.

Step 5: Present Your Results

The findings of hypothesis testing will be discussed in the results and discussion portions of your research paper, dissertation, or thesis. You should include a concise overview of the data and a summary of the findings of your statistical test in the results section. You can talk about whether your results confirmed your initial hypothesis or not in the conversation. Rejecting or failing to reject the null hypothesis is a formal term used in hypothesis testing. This is likely a must for your statistics assignments.

Types of Hypothesis Testing

To determine whether a discovery or relationship is statistically significant, hypothesis testing uses a z-test. It usually checks to see if two means are the same (the null hypothesis). Only when the population standard deviation is known and the sample size is 30 data points or more, can a z-test be applied.

A statistical test called a t-test is employed to compare the means of two groups. To determine whether two groups differ or if a procedure or treatment affects the population of interest, it is frequently used in hypothesis testing.

Chi-Square

You utilize a Chi-square test for hypothesis testing concerning whether your data is as predicted. To determine if the expected and observed results are well-fitted, the Chi-square test analyzes the differences between categorical variables from a random sample. The test's fundamental premise is that the observed values in your data should be compared to the predicted values that would be present if the null hypothesis were true.

Hypothesis Testing and Confidence Intervals

Both confidence intervals and hypothesis tests are inferential techniques that depend on approximating the sample distribution. Data from a sample is used to estimate a population parameter using confidence intervals. Data from a sample is used in hypothesis testing to examine a given hypothesis. We must have a postulated parameter to conduct hypothesis testing.

Bootstrap distributions and randomization distributions are created using comparable simulation techniques. The observed sample statistic is the focal point of a bootstrap distribution, whereas the null hypothesis value is the focal point of a randomization distribution.

A variety of feasible population parameter estimates are included in confidence ranges. In this lesson, we created just two-tailed confidence intervals. There is a direct connection between these two-tail confidence intervals and these two-tail hypothesis tests. The results of a two-tailed hypothesis test and two-tailed confidence intervals typically provide the same results. In other words, a hypothesis test at the 0.05 level will virtually always fail to reject the null hypothesis if the 95% confidence interval contains the predicted value. A hypothesis test at the 0.05 level will nearly certainly reject the null hypothesis if the 95% confidence interval does not include the hypothesized parameter.

Simple and Composite Hypothesis Testing

Depending on the population distribution, you can classify the statistical hypothesis into two types.

Simple Hypothesis: A simple hypothesis specifies an exact value for the parameter.

Composite Hypothesis: A composite hypothesis specifies a range of values.

A company is claiming that their average sales for this quarter are 1000 units. This is an example of a simple hypothesis.

Suppose the company claims that the sales are in the range of 900 to 1000 units. Then this is a case of a composite hypothesis.

One-Tailed and Two-Tailed Hypothesis Testing

The One-Tailed test, also called a directional test, considers a critical region of data that would result in the null hypothesis being rejected if the test sample falls into it, inevitably meaning the acceptance of the alternate hypothesis.

In a one-tailed test, the critical distribution area is one-sided, meaning the test sample is either greater or lesser than a specific value.

In two tails, the test sample is checked to be greater or less than a range of values in a Two-Tailed test, implying that the critical distribution area is two-sided.

If the sample falls within this range, the alternate hypothesis will be accepted, and the null hypothesis will be rejected.

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Right Tailed Hypothesis Testing

If the larger than (>) sign appears in your hypothesis statement, you are using a right-tailed test, also known as an upper test. Or, to put it another way, the disparity is to the right. For instance, you can contrast the battery life before and after a change in production. Your hypothesis statements can be the following if you want to know if the battery life is longer than the original (let's say 90 hours):

The null hypothesis is (H0 <= 90) or less change.
A possibility is that battery life has risen (H1) > 90.

The crucial point in this situation is that the alternate hypothesis (H1), not the null hypothesis, decides whether you get a right-tailed test.

Left Tailed Hypothesis Testing

Alternative hypotheses that assert the true value of a parameter is lower than the null hypothesis are tested with a left-tailed test; they are indicated by the asterisk "<".

Suppose H0: mean = 50 and H1: mean not equal to 50

According to the H1, the mean can be greater than or less than 50. This is an example of a Two-tailed test.

In a similar manner, if H0: mean >=50, then H1: mean <50

Here the mean is less than 50. It is called a One-tailed test.

Type 1 and Type 2 Error

A hypothesis test can result in two types of errors.

Type 1 Error: A Type-I error occurs when sample results reject the null hypothesis despite being true.

Type 2 Error: A Type-II error occurs when the null hypothesis is not rejected when it is false, unlike a Type-I error.

Suppose a teacher evaluates the examination paper to decide whether a student passes or fails.

H0: Student has passed

H1: Student has failed

Type I error will be the teacher failing the student [rejects H0] although the student scored the passing marks [H0 was true].

Type II error will be the case where the teacher passes the student [do not reject H0] although the student did not score the passing marks [H1 is true].

Level of Significance

The alpha value is a criterion for determining whether a test statistic is statistically significant. In a statistical test, Alpha represents an acceptable probability of a Type I error. Because alpha is a probability, it can be anywhere between 0 and 1. In practice, the most commonly used alpha values are 0.01, 0.05, and 0.1, which represent a 1%, 5%, and 10% chance of a Type I error, respectively (i.e. rejecting the null hypothesis when it is in fact correct).

Future-Proof Your AI/ML Career: Top Dos and Don'ts

A p-value is a metric that expresses the likelihood that an observed difference could have occurred by chance. As the p-value decreases the statistical significance of the observed difference increases. If the p-value is too low, you reject the null hypothesis.

Here you have taken an example in which you are trying to test whether the new advertising campaign has increased the product's sales. The p-value is the likelihood that the null hypothesis, which states that there is no change in the sales due to the new advertising campaign, is true. If the p-value is .30, then there is a 30% chance that there is no increase or decrease in the product's sales. If the p-value is 0.03, then there is a 3% probability that there is no increase or decrease in the sales value due to the new advertising campaign. As you can see, the lower the p-value, the chances of the alternate hypothesis being true increases, which means that the new advertising campaign causes an increase or decrease in sales.

Why is Hypothesis Testing Important in Research Methodology?

Hypothesis testing is crucial in research methodology for several reasons:

Provides evidence-based conclusions: It allows researchers to make objective conclusions based on empirical data, providing evidence to support or refute their research hypotheses.
Supports decision-making: It helps make informed decisions, such as accepting or rejecting a new treatment, implementing policy changes, or adopting new practices.
Adds rigor and validity: It adds scientific rigor to research using statistical methods to analyze data, ensuring that conclusions are based on sound statistical evidence.
Contributes to the advancement of knowledge: By testing hypotheses, researchers contribute to the growth of knowledge in their respective fields by confirming existing theories or discovering new patterns and relationships.

Limitations of Hypothesis Testing

Hypothesis testing has some limitations that researchers should be aware of:

It cannot prove or establish the truth: Hypothesis testing provides evidence to support or reject a hypothesis, but it cannot confirm the absolute truth of the research question.
Results are sample-specific: Hypothesis testing is based on analyzing a sample from a population, and the conclusions drawn are specific to that particular sample.
Possible errors: During hypothesis testing, there is a chance of committing type I error (rejecting a true null hypothesis) or type II error (failing to reject a false null hypothesis).
Assumptions and requirements: Different tests have specific assumptions and requirements that must be met to accurately interpret results.

After reading this tutorial, you would have a much better understanding of hypothesis testing, one of the most important concepts in the field of Data Science . The majority of hypotheses are based on speculation about observed behavior, natural phenomena, or established theories.

If you are interested in statistics of data science and skills needed for such a career, you ought to explore Simplilearn’s Post Graduate Program in Data Science.

If you have any questions regarding this ‘Hypothesis Testing In Statistics’ tutorial, do share them in the comment section. Our subject matter expert will respond to your queries. Happy learning!

1. What is hypothesis testing in statistics with example?

Hypothesis testing is a statistical method used to determine if there is enough evidence in a sample data to draw conclusions about a population. It involves formulating two competing hypotheses, the null hypothesis (H0) and the alternative hypothesis (Ha), and then collecting data to assess the evidence. An example: testing if a new drug improves patient recovery (Ha) compared to the standard treatment (H0) based on collected patient data.

2. What is hypothesis testing and its types?

Hypothesis testing is a statistical method used to make inferences about a population based on sample data. It involves formulating two hypotheses: the null hypothesis (H0), which represents the default assumption, and the alternative hypothesis (Ha), which contradicts H0. The goal is to assess the evidence and determine whether there is enough statistical significance to reject the null hypothesis in favor of the alternative hypothesis.

Types of hypothesis testing:

One-sample test: Used to compare a sample to a known value or a hypothesized value.
Two-sample test: Compares two independent samples to assess if there is a significant difference between their means or distributions.
Paired-sample test: Compares two related samples, such as pre-test and post-test data, to evaluate changes within the same subjects over time or under different conditions.
Chi-square test: Used to analyze categorical data and determine if there is a significant association between variables.
ANOVA (Analysis of Variance): Compares means across multiple groups to check if there is a significant difference between them.

3. What are the steps of hypothesis testing?

The steps of hypothesis testing are as follows:

Formulate the hypotheses: State the null hypothesis (H0) and the alternative hypothesis (Ha) based on the research question.
Set the significance level: Determine the acceptable level of error (alpha) for making a decision.
Collect and analyze data: Gather and process the sample data.
Compute test statistic: Calculate the appropriate statistical test to assess the evidence.
Make a decision: Compare the test statistic with critical values or p-values and determine whether to reject H0 in favor of Ha or not.
Draw conclusions: Interpret the results and communicate the findings in the context of the research question.

4. What are the 2 types of hypothesis testing?

One-tailed (or one-sided) test: Tests for the significance of an effect in only one direction, either positive or negative.
Two-tailed (or two-sided) test: Tests for the significance of an effect in both directions, allowing for the possibility of a positive or negative effect.

The choice between one-tailed and two-tailed tests depends on the specific research question and the directionality of the expected effect.

5. What are the 3 major types of hypothesis?

The three major types of hypotheses are:

Null Hypothesis (H0): Represents the default assumption, stating that there is no significant effect or relationship in the data.
Alternative Hypothesis (Ha): Contradicts the null hypothesis and proposes a specific effect or relationship that researchers want to investigate.
Nondirectional Hypothesis: An alternative hypothesis that doesn't specify the direction of the effect, leaving it open for both positive and negative possibilities.

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Elementary Statistical Methods pp 147–240 Cite as

Hypothesis Testing

Sahana Prasad 2
First Online: 06 February 2023

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As the world starts processing data, decisions become more data-centred, and hypothesis testing is very crucial in this procedure. It helps to extend conclusions based on sample to the population and also test if any changes in the sample statistics are significant or due to sheer chance. It is a statistical analysis tool, which draws conclusion about a given population value, association of variables and significance testing. There are two types of hypothesis, the null and alternate hypotheses. In addition, we have to understand the concepts of errors, level of significance, power of a test and others to make use of this concept.

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Other References

Acceptance and rejection regions : https://ani.stat.fsu.edu/~debdeep/p10.pdf

One and two tailed tests : https://www.sciencedirect.com/topics/mathematics/tailed-test

p-value : https://www.simplypsychology.org/p-value.html

Real life problems translated into hypothesis language : https://www.researchgate.net/figure/2-Three-Approaches-of-Hypothesis-Testing-16_tbl6_263846563

Errors and types of errors : https://bit.ly/2XFv6oh , https://online.datasciencedojo.com/blogs/type-i-type-ii-errors/ : Casey Anthony, O.J. Simpson and other examples attempt to minimize the extent to which innocent individuals are falsely convicted

Scandal on a medical device maker : Adapted from European Journal of Epidemiology, April; 25(4): 223–224. Curbing type I and type II errors by Kenneth J. Rothman .

Google Scholar

Between and within variations : https://bookdown.org/BaktiSiregar/data-science-for-beginners-part-2/11-ANOVA.html

Post Hoc Tests : https://bit.ly/3mxISBZ

Tukey’s simultaneous 95% CI’s : https://www.researchgate.net/figure/Tukey-Simultaneous-95-Confidence-Intervals-for-Taber-Abrasion-Resistance-Coating_fig25_303719917

Binomial tests : https://math.stackexchange.com/questions/2123873/is-the-maximum-of-a-probability-distribution-function-of-a-binomial-distribution

Goodness of fit : https://www.akira.ai/glossary/goodness-of-fit/

Interpretation of Cochran’s Q test : https://www.scalestatistics.com/cochrans-q.html

Comic on Estimation : https://comicsidontunderstand.com/tag/frank-ernest/

When to use which test? https://www.scirp.org/journal/paperinformation.aspx?paperid=109618

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Hypothesis Testing Terminology

Posted by Ted Hessing

When performing a hypothesis test, you are likely to encounter terms with which you may not be familiar. Unfortunately, this could hamper your ability to carry out hypothesis testing in a meaningful way. Likewise, you will find it hard to communicate your results to the stakeholders without the appropriate vocabulary. To avoid these issues, brushing up on your hypothesis-testing terminology is important. Let’s take a look at the most commonly used terms.

Alpha level

Also known as the alpha risk. The risk of committing a Type A error or incorrectly rejecting your null hypothesis is acceptable. An alpha level is always a number between 0 and 1—most commonly, people use a value of 0.05. Once your test is complete and you’ve run the data through statistical software, you’ll have a p-value to compare to your alpha level.

Alternative hypothesis

A hypothesis that disagrees with the null hypothesis ; the two are mutually exclusive.

Also known as the beta risk. It’s the acceptable risk of committing a Type B error–i.e., not rejecting your null hypothesis when it is, in fact, incorrect.

A statement that depicts the level of evidence (sufficient or insufficient), at what level of significance, and whether you reject (null) or support (alternative) the original claim.

Confidence level

Also known as the confidence interval. This refers to how confident you can be that your conclusion is, in fact, correct. The confidence level is easy to calculate: the alpha and confidence levels always add up to one. ie:

1 – α = confidence level

Critical region

Set of all values which would cause us to reject the null hypothesis H 0 . Also known as a rejection region.

Critical value(s)

The value(s) which separate the critical region from the non-critical region. The critical values are determined independently of the sample statistics.

A critical value separates the rejection region from the non-rejection region.

A statement based upon the null hypothesis. It is either “reject the null hypothesis” or “fail to reject the null hypothesis.” We will never accept the null hypothesis.

A p-value is the probability of getting a test statistic that is at least as extreme as the one found in the sample data.

Two basic error types occur in hypothesis testing: type A errors, where you reject a correct hypothesis, and type B errors, where you accept an incorrect hypothesis. Read more about errors .

Also known as the null hypothesis .

Also known as the alternative hypothesis , or H(a).

Left-tailed test

The hypothesis test is left-tailed if the alternative hypothesis H1 contains the less-than inequality symbol (<).

Null hypothesis

The statement that you’re trying to disprove. Generally, this is the assumption that the experimental results are due to chance alone; nothing else influenced the results.

A p-value is a crucial element of any hypothesis test results. It’s a number between 0 and 1, and it gauges the probability that random fluctuations caused any data that might cause you to reject the null hypothesis. It’s calculated by running test results through a statistical significance test. If the p-value is lower than your alpha level, then you reject the null hypothesis. If higher, then you do not reject the null hypothesis. Read more about p-values .

Pooled (vs. Unpooled)

Pooling refers to the way in which the standard error is estimated when calculating terms for a hypothesis test.

The two proportions are averaged in the pooled version, and only one proportion is used to estimate the standard error. ASQ, Villanova, and most other organizations favor pooled calculations.

In the unpooled version, the two proportions are used separately. IASSC generally favors unpooled.

Here’s a good read on when to use which in practice .

Rejection region

Also known as a critical region .

Right-tailed test

If the alternative hypothesis H1 contains the greater-than-inequality symbol (>), the hypothesis test is right-tailed.

In hypothesis testing, when performing a right-tailed test, we reject the null hypothesis if the test statistic is larger than the critical value.
Only when the test statistic is larger than the critical value will we be able to reject the null? Usually (emphasis on “usually”), we hope to reject the null because that means that our efforts are not in vain. If you are testing the null hypothesis and you are hoping that you have not adversely affected the process, you would then be hoping NOT to reject the null.

Significance level

Also known as the alpha level .

The probability of rejecting the null hypothesis when it is true: alpha = 0.05 and alpha = 0.01 are common. If no level of significance is given, use alpha = 0.05. The level of significance is the complement of the level of confidence in estimation.

The significance level (denoted by Alpha) is the probability that the test statistic will fall in the critical region when the null hypothesis is actually true.

Test statistic

A sample statistic is used to decide whether to reject or fail to reject the null hypothesis.

Two-Tailed Test

A two-tailed test is one with two rejection regions . If the null hypothesis has an equal sign, then this is a two-tailed test, and you can use the test statistic to reject the null hypothesis if the test statistic is too large or too small.

H0: µnew = µcurrent

Ha: µnew ≠ µcurrent

H0: µ new = µ current Ha: µ new is not = µ current

For example, if the null hypothesis has an equal sign, then this is a two-tailed test, and you can use the test statistic to reject the null hypothesis if the test statistic is too large or too small.

Comments (7)

Good stuff. Thanks allot.

Glad it helps, Chan!

Do we have to memorize all the formulas for the exam?

This depends on the organization you’re preparing for.

ASQ is open book. So, for organizations like that you don’t have to memorize, but you do have to collect all of the possible reference material you might want ahead of time.

IASSC is NOT open book. But they provide a reference sheet and you’re not allowed anything else. You can request a copy of the reference sheet from them.

Hi, what would be the probability of the test statistic falling in the critical region, whether the null hypothesis is true or false?

Sam, are you referring to the significance level (alpha)?

H0 or Null hypothesis, from my understanding, I assumed it represented the true value or outcome that we hypothesize that we are trying to prove or disprove with experimentation. But from your definition it seems almost like the opposite, and that you are trying to disprove this hypothesis.

My idea of a Null hypothesis would be hypothesizing that combining a combination of metal 1 and metal 2 to make a compound metal would increase tensile strength, and it achieving that result. Therefore the Null Hypothesis was proven correct.

Is this understanding correct? Could you please help give me some clarification or an example of one?

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Ind Psychiatry J
v.18(2); Jul-Dec 2009

Hypothesis testing, type I and type II errors

Amitav banerjee.

Department of Community Medicine, D. Y. Patil Medical College, Pune, India

U. B. Chitnis

S. l. jadhav, j. s. bhawalkar, s. chaudhury.

1 Department of Psychiatry, RINPAS, Kanke, Ranchi, India

Hypothesis testing is an important activity of empirical research and evidence-based medicine. A well worked up hypothesis is half the answer to the research question. For this, both knowledge of the subject derived from extensive review of the literature and working knowledge of basic statistical concepts are desirable. The present paper discusses the methods of working up a good hypothesis and statistical concepts of hypothesis testing.

Karl Popper is probably the most influential philosopher of science in the 20 th century (Wulff et al ., 1986). Many scientists, even those who do not usually read books on philosophy, are acquainted with the basic principles of his views on science. The popularity of Popper’s philosophy is due partly to the fact that it has been well explained in simple terms by, among others, the Nobel Prize winner Peter Medawar (Medawar, 1969). Popper makes the very important point that empirical scientists (those who stress on observations only as the starting point of research) put the cart in front of the horse when they claim that science proceeds from observation to theory, since there is no such thing as a pure observation which does not depend on theory. Popper states, “… the belief that we can start with pure observation alone, without anything in the nature of a theory, is absurd: As may be illustrated by the story of the man who dedicated his life to natural science, wrote down everything he could observe, and bequeathed his ‘priceless’ collection of observations to the Royal Society to be used as inductive (empirical) evidence.

STARTING POINT OF RESEARCH: HYPOTHESIS OR OBSERVATION?

The first step in the scientific process is not observation but the generation of a hypothesis which may then be tested critically by observations and experiments. Popper also makes the important claim that the goal of the scientist’s efforts is not the verification but the falsification of the initial hypothesis. It is logically impossible to verify the truth of a general law by repeated observations, but, at least in principle, it is possible to falsify such a law by a single observation. Repeated observations of white swans did not prove that all swans are white, but the observation of a single black swan sufficed to falsify that general statement (Popper, 1976).

CHARACTERISTICS OF A GOOD HYPOTHESIS

A good hypothesis must be based on a good research question. It should be simple, specific and stated in advance (Hulley et al ., 2001).

Hypothesis should be simple

A simple hypothesis contains one predictor and one outcome variable, e.g. positive family history of schizophrenia increases the risk of developing the condition in first-degree relatives. Here the single predictor variable is positive family history of schizophrenia and the outcome variable is schizophrenia. A complex hypothesis contains more than one predictor variable or more than one outcome variable, e.g., a positive family history and stressful life events are associated with an increased incidence of Alzheimer’s disease. Here there are 2 predictor variables, i.e., positive family history and stressful life events, while one outcome variable, i.e., Alzheimer’s disease. Complex hypothesis like this cannot be easily tested with a single statistical test and should always be separated into 2 or more simple hypotheses.

Hypothesis should be specific

A specific hypothesis leaves no ambiguity about the subjects and variables, or about how the test of statistical significance will be applied. It uses concise operational definitions that summarize the nature and source of the subjects and the approach to measuring variables (History of medication with tranquilizers, as measured by review of medical store records and physicians’ prescriptions in the past year, is more common in patients who attempted suicides than in controls hospitalized for other conditions). This is a long-winded sentence, but it explicitly states the nature of predictor and outcome variables, how they will be measured and the research hypothesis. Often these details may be included in the study proposal and may not be stated in the research hypothesis. However, they should be clear in the mind of the investigator while conceptualizing the study.

Hypothesis should be stated in advance

The hypothesis must be stated in writing during the proposal state. This will help to keep the research effort focused on the primary objective and create a stronger basis for interpreting the study’s results as compared to a hypothesis that emerges as a result of inspecting the data. The habit of post hoc hypothesis testing (common among researchers) is nothing but using third-degree methods on the data (data dredging), to yield at least something significant. This leads to overrating the occasional chance associations in the study.

TYPES OF HYPOTHESES

For the purpose of testing statistical significance, hypotheses are classified by the way they describe the expected difference between the study groups.

Null and alternative hypotheses

The null hypothesis states that there is no association between the predictor and outcome variables in the population (There is no difference between tranquilizer habits of patients with attempted suicides and those of age- and sex- matched “control” patients hospitalized for other diagnoses). The null hypothesis is the formal basis for testing statistical significance. By starting with the proposition that there is no association, statistical tests can estimate the probability that an observed association could be due to chance.

The proposition that there is an association — that patients with attempted suicides will report different tranquilizer habits from those of the controls — is called the alternative hypothesis. The alternative hypothesis cannot be tested directly; it is accepted by exclusion if the test of statistical significance rejects the null hypothesis.

One- and two-tailed alternative hypotheses

A one-tailed (or one-sided) hypothesis specifies the direction of the association between the predictor and outcome variables. The prediction that patients of attempted suicides will have a higher rate of use of tranquilizers than control patients is a one-tailed hypothesis. A two-tailed hypothesis states only that an association exists; it does not specify the direction. The prediction that patients with attempted suicides will have a different rate of tranquilizer use — either higher or lower than control patients — is a two-tailed hypothesis. (The word tails refers to the tail ends of the statistical distribution such as the familiar bell-shaped normal curve that is used to test a hypothesis. One tail represents a positive effect or association; the other, a negative effect.) A one-tailed hypothesis has the statistical advantage of permitting a smaller sample size as compared to that permissible by a two-tailed hypothesis. Unfortunately, one-tailed hypotheses are not always appropriate; in fact, some investigators believe that they should never be used. However, they are appropriate when only one direction for the association is important or biologically meaningful. An example is the one-sided hypothesis that a drug has a greater frequency of side effects than a placebo; the possibility that the drug has fewer side effects than the placebo is not worth testing. Whatever strategy is used, it should be stated in advance; otherwise, it would lack statistical rigor. Data dredging after it has been collected and post hoc deciding to change over to one-tailed hypothesis testing to reduce the sample size and P value are indicative of lack of scientific integrity.

STATISTICAL PRINCIPLES OF HYPOTHESIS TESTING

A hypothesis (for example, Tamiflu [oseltamivir], drug of choice in H1N1 influenza, is associated with an increased incidence of acute psychotic manifestations) is either true or false in the real world. Because the investigator cannot study all people who are at risk, he must test the hypothesis in a sample of that target population. No matter how many data a researcher collects, he can never absolutely prove (or disprove) his hypothesis. There will always be a need to draw inferences about phenomena in the population from events observed in the sample (Hulley et al ., 2001). In some ways, the investigator’s problem is similar to that faced by a judge judging a defendant [ Table 1 ]. The absolute truth whether the defendant committed the crime cannot be determined. Instead, the judge begins by presuming innocence — the defendant did not commit the crime. The judge must decide whether there is sufficient evidence to reject the presumed innocence of the defendant; the standard is known as beyond a reasonable doubt. A judge can err, however, by convicting a defendant who is innocent, or by failing to convict one who is actually guilty. In similar fashion, the investigator starts by presuming the null hypothesis, or no association between the predictor and outcome variables in the population. Based on the data collected in his sample, the investigator uses statistical tests to determine whether there is sufficient evidence to reject the null hypothesis in favor of the alternative hypothesis that there is an association in the population. The standard for these tests is shown as the level of statistical significance.

The analogy between judge’s decisions and statistical tests

TYPE I (ALSO KNOWN AS ‘α’) AND TYPE II (ALSO KNOWN AS ‘β’)ERRORS

Just like a judge’s conclusion, an investigator’s conclusion may be wrong. Sometimes, by chance alone, a sample is not representative of the population. Thus the results in the sample do not reflect reality in the population, and the random error leads to an erroneous inference. A type I error (false-positive) occurs if an investigator rejects a null hypothesis that is actually true in the population; a type II error (false-negative) occurs if the investigator fails to reject a null hypothesis that is actually false in the population. Although type I and type II errors can never be avoided entirely, the investigator can reduce their likelihood by increasing the sample size (the larger the sample, the lesser is the likelihood that it will differ substantially from the population).

False-positive and false-negative results can also occur because of bias (observer, instrument, recall, etc.). (Errors due to bias, however, are not referred to as type I and type II errors.) Such errors are troublesome, since they may be difficult to detect and cannot usually be quantified.

EFFECT SIZE

The likelihood that a study will be able to detect an association between a predictor variable and an outcome variable depends, of course, on the actual magnitude of that association in the target population. If it is large (such as 90% increase in the incidence of psychosis in people who are on Tamiflu), it will be easy to detect in the sample. Conversely, if the size of the association is small (such as 2% increase in psychosis), it will be difficult to detect in the sample. Unfortunately, the investigator often does not know the actual magnitude of the association — one of the purposes of the study is to estimate it. Instead, the investigator must choose the size of the association that he would like to be able to detect in the sample. This quantity is known as the effect size. Selecting an appropriate effect size is the most difficult aspect of sample size planning. Sometimes, the investigator can use data from other studies or pilot tests to make an informed guess about a reasonable effect size. When there are no data with which to estimate it, he can choose the smallest effect size that would be clinically meaningful, for example, a 10% increase in the incidence of psychosis. Of course, from the public health point of view, even a 1% increase in psychosis incidence would be important. Thus the choice of the effect size is always somewhat arbitrary, and considerations of feasibility are often paramount. When the number of available subjects is limited, the investigator may have to work backward to determine whether the effect size that his study will be able to detect with that number of subjects is reasonable.

α,β,AND POWER

After a study is completed, the investigator uses statistical tests to try to reject the null hypothesis in favor of its alternative (much in the same way that a prosecuting attorney tries to convince a judge to reject innocence in favor of guilt). Depending on whether the null hypothesis is true or false in the target population, and assuming that the study is free of bias, 4 situations are possible, as shown in Table 2 below. In 2 of these, the findings in the sample and reality in the population are concordant, and the investigator’s inference will be correct. In the other 2 situations, either a type I (α) or a type II (β) error has been made, and the inference will be incorrect.

Truth in the population versus the results in the study sample: The four possibilities

The investigator establishes the maximum chance of making type I and type II errors in advance of the study. The probability of committing a type I error (rejecting the null hypothesis when it is actually true) is called α (alpha) the other name for this is the level of statistical significance.

If a study of Tamiflu and psychosis is designed with α = 0.05, for example, then the investigator has set 5% as the maximum chance of incorrectly rejecting the null hypothesis (and erroneously inferring that use of Tamiflu and psychosis incidence are associated in the population). This is the level of reasonable doubt that the investigator is willing to accept when he uses statistical tests to analyze the data after the study is completed.

The probability of making a type II error (failing to reject the null hypothesis when it is actually false) is called β (beta). The quantity (1 - β) is called power, the probability of observing an effect in the sample (if one), of a specified effect size or greater exists in the population.

If β is set at 0.10, then the investigator has decided that he is willing to accept a 10% chance of missing an association of a given effect size between Tamiflu and psychosis. This represents a power of 0.90, i.e., a 90% chance of finding an association of that size. For example, suppose that there really would be a 30% increase in psychosis incidence if the entire population took Tamiflu. Then 90 times out of 100, the investigator would observe an effect of that size or larger in his study. This does not mean, however, that the investigator will be absolutely unable to detect a smaller effect; just that he will have less than 90% likelihood of doing so.

Ideally alpha and beta errors would be set at zero, eliminating the possibility of false-positive and false-negative results. In practice they are made as small as possible. Reducing them, however, usually requires increasing the sample size. Sample size planning aims at choosing a sufficient number of subjects to keep alpha and beta at acceptably low levels without making the study unnecessarily expensive or difficult.

Many studies s et al pha at 0.05 and beta at 0.20 (a power of 0.80). These are somewhat arbitrary values, and others are sometimes used; the conventional range for alpha is between 0.01 and 0.10; and for beta, between 0.05 and 0.20. In general the investigator should choose a low value of alpha when the research question makes it particularly important to avoid a type I (false-positive) error, and he should choose a low value of beta when it is especially important to avoid a type II error.

The null hypothesis acts like a punching bag: It is assumed to be true in order to shadowbox it into false with a statistical test. When the data are analyzed, such tests determine the P value, the probability of obtaining the study results by chance if the null hypothesis is true. The null hypothesis is rejected in favor of the alternative hypothesis if the P value is less than alpha, the predetermined level of statistical significance (Daniel, 2000). “Nonsignificant” results — those with P value greater than alpha — do not imply that there is no association in the population; they only mean that the association observed in the sample is small compared with what could have occurred by chance alone. For example, an investigator might find that men with family history of mental illness were twice as likely to develop schizophrenia as those with no family history, but with a P value of 0.09. This means that even if family history and schizophrenia were not associated in the population, there was a 9% chance of finding such an association due to random error in the sample. If the investigator had set the significance level at 0.05, he would have to conclude that the association in the sample was “not statistically significant.” It might be tempting for the investigator to change his mind about the level of statistical significance ex post facto and report the results “showed statistical significance at P < 10”. A better choice would be to report that the “results, although suggestive of an association, did not achieve statistical significance ( P = .09)”. This solution acknowledges that statistical significance is not an “all or none” situation.

Hypothesis testing is the sheet anchor of empirical research and in the rapidly emerging practice of evidence-based medicine. However, empirical research and, ipso facto, hypothesis testing have their limits. The empirical approach to research cannot eliminate uncertainty completely. At the best, it can quantify uncertainty. This uncertainty can be of 2 types: Type I error (falsely rejecting a null hypothesis) and type II error (falsely accepting a null hypothesis). The acceptable magnitudes of type I and type II errors are set in advance and are important for sample size calculations. Another important point to remember is that we cannot ‘prove’ or ‘disprove’ anything by hypothesis testing and statistical tests. We can only knock down or reject the null hypothesis and by default accept the alternative hypothesis. If we fail to reject the null hypothesis, we accept it by default.

Source of Support: Nil

Conflict of Interest: None declared.

Daniel W. W. In: Biostatistics. 7th ed. New York: John Wiley and Sons, Inc; 2002. Hypothesis testing; pp. 204–294. [ Google Scholar ]
Hulley S. B, Cummings S. R, Browner W. S, Grady D, Hearst N, Newman T. B. 2nd ed. Philadelphia: Lippincott Williams and Wilkins; 2001. Getting ready to estimate sample size: Hypothesis and underlying principles In: Designing Clinical Research-An epidemiologic approach; pp. 51–63. [ Google Scholar ]
Medawar P. B. Philadelphia: American Philosophical Society; 1969. Induction and intuition in scientific thought. [ Google Scholar ]
Popper K. Unended Quest. An Intellectual Autobiography. Fontana Collins; p. 42. [ Google Scholar ]
Wulff H. R, Pedersen S. A, Rosenberg R. Oxford: Blackwell Scientific Publicatons; Empirism and Realism: A philosophical problem. In: Philosophy of Medicine. [ Google Scholar ]

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Hypothesis testing involves formulating assumptions about population parameters based on sample statistics and rigorously evaluating these assumptions against empirical evidence. This article sheds light on the significance of hypothesis testing and the critical steps involved in the process.

What is Hypothesis Testing?

Hypothesis testing is a statistical method that is used to make a statistical decision using experimental data. Hypothesis testing is basically an assumption that we make about a population parameter. It evaluates two mutually exclusive statements about a population to determine which statement is best supported by the sample data.

Example: You say an average height in the class is 30 or a boy is taller than a girl. All of these is an assumption that we are assuming, and we need some statistical way to prove these. We need some mathematical conclusion whatever we are assuming is true.

Defining Hypotheses

$\mu$

Key Terms of Hypothesis Testing

$\alpha$

P-value: The P value , or calculated probability, is the probability of finding the observed/extreme results when the null hypothesis(H0) of a study-given problem is true. If your P-value is less than the chosen significance level then you reject the null hypothesis i.e. accept that your sample claims to support the alternative hypothesis.
Test Statistic: The test statistic is a numerical value calculated from sample data during a hypothesis test, used to determine whether to reject the null hypothesis. It is compared to a critical value or p-value to make decisions about the statistical significance of the observed results.
Critical value : The critical value in statistics is a threshold or cutoff point used to determine whether to reject the null hypothesis in a hypothesis test.
Degrees of freedom: Degrees of freedom are associated with the variability or freedom one has in estimating a parameter. The degrees of freedom are related to the sample size and determine the shape.

Why do we use Hypothesis Testing?

Hypothesis testing is an important procedure in statistics. Hypothesis testing evaluates two mutually exclusive population statements to determine which statement is most supported by sample data. When we say that the findings are statistically significant, thanks to hypothesis testing.

One-Tailed and Two-Tailed Test

One tailed test focuses on one direction, either greater than or less than a specified value. We use a one-tailed test when there is a clear directional expectation based on prior knowledge or theory. The critical region is located on only one side of the distribution curve. If the sample falls into this critical region, the null hypothesis is rejected in favor of the alternative hypothesis.

One-Tailed Test

There are two types of one-tailed test:

$\mu \geq 50$

Two-Tailed Test

A two-tailed test considers both directions, greater than and less than a specified value.We use a two-tailed test when there is no specific directional expectation, and want to detect any significant difference.

$\mu =$

What are Type 1 and Type 2 errors in Hypothesis Testing?

In hypothesis testing, Type I and Type II errors are two possible errors that researchers can make when drawing conclusions about a population based on a sample of data. These errors are associated with the decisions made regarding the null hypothesis and the alternative hypothesis.

$\alpha$

How does Hypothesis Testing work?

Step 1: define null and alternative hypothesis.

We first identify the problem about which we want to make an assumption keeping in mind that our assumption should be contradictory to one another, assuming Normally distributed data.

Step 2 – Choose significance level

$\alpha$

Step 3 – Collect and Analyze data.

Gather relevant data through observation or experimentation. Analyze the data using appropriate statistical methods to obtain a test statistic.

Step 4-Calculate Test Statistic

The data for the tests are evaluated in this step we look for various scores based on the characteristics of data. The choice of the test statistic depends on the type of hypothesis test being conducted.

There are various hypothesis tests, each appropriate for various goal to calculate our test. This could be a Z-test , Chi-square , T-test , and so on.

Z-test : If population means and standard deviations are known. Z-statistic is commonly used.
t-test : If population standard deviations are unknown. and sample size is small than t-test statistic is more appropriate.
Chi-square test : Chi-square test is used for categorical data or for testing independence in contingency tables
F-test : F-test is often used in analysis of variance (ANOVA) to compare variances or test the equality of means across multiple groups.

We have a smaller dataset, So, T-test is more appropriate to test our hypothesis.

T-statistic is a measure of the difference between the means of two groups relative to the variability within each group. It is calculated as the difference between the sample means divided by the standard error of the difference. It is also known as the t-value or t-score.

Step 5 – Comparing Test Statistic:

In this stage, we decide where we should accept the null hypothesis or reject the null hypothesis. There are two ways to decide where we should accept or reject the null hypothesis.

Method A: Using Crtical values

Comparing the test statistic and tabulated critical value we have,

If Test Statistic>Critical Value: Reject the null hypothesis.
If Test Statistic≤Critical Value: Fail to reject the null hypothesis.

Note: Critical values are predetermined threshold values that are used to make a decision in hypothesis testing. To determine critical values for hypothesis testing, we typically refer to a statistical distribution table , such as the normal distribution or t-distribution tables based on.

Method B: Using P-values

We can also come to an conclusion using the p-value,

$p\leq\alpha$

Note : The p-value is the probability of obtaining a test statistic as extreme as, or more extreme than, the one observed in the sample, assuming the null hypothesis is true. To determine p-value for hypothesis testing, we typically refer to a statistical distribution table , such as the normal distribution or t-distribution tables based on.

Step 7- Interpret the Results

At last, we can conclude our experiment using method A or B.

Calculating test statistic

To validate our hypothesis about a population parameter we use statistical functions . We use the z-score, p-value, and level of significance(alpha) to make evidence for our hypothesis for normally distributed data .

1. Z-statistics:

When population means and standard deviations are known.

$z = \frac{\bar{x} - \mu}{\frac{\sigma}{\sqrt{n}}}$

μ represents the population mean,
σ is the standard deviation
and n is the size of the sample.

2. T-Statistics

T test is used when n<30,

t-statistic calculation is given by:

$t=\frac{x̄-μ}{s/\sqrt{n}}$

t = t-score,
x̄ = sample mean
μ = population mean,
s = standard deviation of the sample,
n = sample size

3. Chi-Square Test

Chi-Square Test for Independence categorical Data (Non-normally distributed) using:

$\chi^2 = \sum \frac{(O_{ij} - E_{ij})^2}{E_{ij}}$

i,j are the rows and columns index respectively.

$E_{ij}$

Real life Hypothesis Testing example

Let’s examine hypothesis testing using two real life situations,

Case A: D oes a New Drug Affect Blood Pressure?

Imagine a pharmaceutical company has developed a new drug that they believe can effectively lower blood pressure in patients with hypertension. Before bringing the drug to market, they need to conduct a study to assess its impact on blood pressure.

Before Treatment: 120, 122, 118, 130, 125, 128, 115, 121, 123, 119
After Treatment: 115, 120, 112, 128, 122, 125, 110, 117, 119, 114

Step 1 : Define the Hypothesis

Null Hypothesis : (H 0 )The new drug has no effect on blood pressure.
Alternate Hypothesis : (H 1 )The new drug has an effect on blood pressure.

Step 2: Define the Significance level

Let’s consider the Significance level at 0.05, indicating rejection of the null hypothesis.

If the evidence suggests less than a 5% chance of observing the results due to random variation.

Step 3 : Compute the test statistic

Using paired T-test analyze the data to obtain a test statistic and a p-value.

The test statistic (e.g., T-statistic) is calculated based on the differences between blood pressure measurements before and after treatment.

t = m/(s/√n)

m = mean of the difference i.e X after, X before
s = standard deviation of the difference (d) i.e d i = X after, i − X before,
n = sample size,

then, m= -3.9, s= 1.8 and n= 10

we, calculate the , T-statistic = -9 based on the formula for paired t test

Step 4: Find the p-value

The calculated t-statistic is -9 and degrees of freedom df = 9, you can find the p-value using statistical software or a t-distribution table.

thus, p-value = 8.538051223166285e-06

Step 5: Result

If the p-value is less than or equal to 0.05, the researchers reject the null hypothesis.
If the p-value is greater than 0.05, they fail to reject the null hypothesis.

Conclusion: Since the p-value (8.538051223166285e-06) is less than the significance level (0.05), the researchers reject the null hypothesis. There is statistically significant evidence that the average blood pressure before and after treatment with the new drug is different.

Python Implementation of Hypothesis Testing

Let’s create hypothesis testing with python, where we are testing whether a new drug affects blood pressure. For this example, we will use a paired T-test. We’ll use the scipy.stats library for the T-test.

Scipy is a mathematical library in Python that is mostly used for mathematical equations and computations.

We will implement our first real life problem via python,

In the above example, given the T-statistic of approximately -9 and an extremely small p-value, the results indicate a strong case to reject the null hypothesis at a significance level of 0.05.

The results suggest that the new drug, treatment, or intervention has a significant effect on lowering blood pressure.
The negative T-statistic indicates that the mean blood pressure after treatment is significantly lower than the assumed population mean before treatment.

Case B : Cholesterol level in a population

Data: A sample of 25 individuals is taken, and their cholesterol levels are measured.

Cholesterol Levels (mg/dL): 205, 198, 210, 190, 215, 205, 200, 192, 198, 205, 198, 202, 208, 200, 205, 198, 205, 210, 192, 205, 198, 205, 210, 192, 205.

Populations Mean = 200

Population Standard Deviation (σ): 5 mg/dL(given for this problem)

Step 1: Define the Hypothesis

Null Hypothesis (H 0 ): The average cholesterol level in a population is 200 mg/dL.
Alternate Hypothesis (H 1 ): The average cholesterol level in a population is different from 200 mg/dL.

As the direction of deviation is not given , we assume a two-tailed test, and based on a normal distribution table, the critical values for a significance level of 0.05 (two-tailed) can be calculated through the z-table and are approximately -1.96 and 1.96.

$(203.8 - 200) / (5 \div \sqrt{25})$

Step 4: Result

Since the absolute value of the test statistic (2.04) is greater than the critical value (1.96), we reject the null hypothesis. And conclude that, there is statistically significant evidence that the average cholesterol level in the population is different from 200 mg/dL

Limitations of Hypothesis Testing

Although a useful technique, hypothesis testing does not offer a comprehensive grasp of the topic being studied. Without fully reflecting the intricacy or whole context of the phenomena, it concentrates on certain hypotheses and statistical significance.
The accuracy of hypothesis testing results is contingent on the quality of available data and the appropriateness of statistical methods used. Inaccurate data or poorly formulated hypotheses can lead to incorrect conclusions.
Relying solely on hypothesis testing may cause analysts to overlook significant patterns or relationships in the data that are not captured by the specific hypotheses being tested. This limitation underscores the importance of complimenting hypothesis testing with other analytical approaches.

Hypothesis testing stands as a cornerstone in statistical analysis, enabling data scientists to navigate uncertainties and draw credible inferences from sample data. By systematically defining null and alternative hypotheses, choosing significance levels, and leveraging statistical tests, researchers can assess the validity of their assumptions. The article also elucidates the critical distinction between Type I and Type II errors, providing a comprehensive understanding of the nuanced decision-making process inherent in hypothesis testing. The real-life example of testing a new drug’s effect on blood pressure using a paired T-test showcases the practical application of these principles, underscoring the importance of statistical rigor in data-driven decision-making.

Frequently Asked Questions (FAQs)

1. what are the 3 types of hypothesis test.

There are three types of hypothesis tests: right-tailed, left-tailed, and two-tailed. Right-tailed tests assess if a parameter is greater, left-tailed if lesser. Two-tailed tests check for non-directional differences, greater or lesser.

2.What are the 4 components of hypothesis testing?

Null Hypothesis ( ): No effect or difference exists. Alternative Hypothesis ( ): An effect or difference exists. Significance Level ( ): Risk of rejecting null hypothesis when it’s true (Type I error). Test Statistic: Numerical value representing observed evidence against null hypothesis.

3.What is hypothesis testing in ML?

Statistical method to evaluate the performance and validity of machine learning models. Tests specific hypotheses about model behavior, like whether features influence predictions or if a model generalizes well to unseen data.

4.What is the difference between Pytest and hypothesis in Python?

Pytest purposes general testing framework for Python code while Hypothesis is a Property-based testing framework for Python, focusing on generating test cases based on specified properties of the code.

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Published: 30 March 2024

Complex effects of testosterone level on ectoparasite load in a ground squirrel: an experimental test for the immunocompetence handicap hypothesis

Li-Qing Wang 1 na1 ,
Zhi-Tao Liu 2 na1 ,
Jian-Jun Wang 3 na1 ,
Yu-Han Fang 4 ,
Hao Zhu 4 ,
Fu-Shun Zhang 1 &
Ling-Ying Shuai 4

Parasites & Vectors volume 17 , Article number: 164 ( 2024 ) Cite this article

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The immunocompetence handicap hypothesis suggests that males with a higher testosterone level should be better at developing male secondary traits, but at a cost of suppressed immune performance. As a result, we should expect that males with an increased testosterone level also possess a higher parasite load. However, previous empirical studies aimed to test this prediction have generated mixed results. Meanwhile, the effect of testosterone level on parasite load in female hosts remains poorly known.

In this study, we tested this prediction by manipulating testosterone level in Daurian ground squirrels ( Spermophilus dauricus ), a medium-sized rodent widely distributed in northeast Asia. S. dauricus is an important host of ticks and fleas and often viewed as a considerable reservoir of plague. Live-trapped S. dauricus were injected with either tea oil (control group) or testosterone (treatment group) and then released. A total of 10 days later, the rodents were recaptured and checked for ectoparasites. Fecal samples were also collected to measure testosterone level of each individual.

We found that testosterone manipulation and sex of hosts interacted to affect tick load. At the end of the experiment, male squirrels subjected to testosterone implantation had an averagely higher tick load than males from the control group. However, this pattern was not found in females. Moreover, testosterone manipulation did not significantly affect flea load in S. dauricus .

Conclusions

Our results only lent limited support for the immunocompetence handicap hypothesis, suggesting that the role of testosterone on regulating parasite load is relatively complex, and may largely depend on parasite type and gender of hosts.

Graphical Abstract

As one of the most fundamental biological processes, parasitism is widespread in terms of both geography and taxonomy [ 1 ]. Although in most cases nonlethal to their hosts, parasites often play important roles in shaping behavior, fitness and population dynamics of hosts [ 2 ], and may even have keystone effects on community structure in some ecosystems [ 3 ]. Meanwhile, many parasites are important transmitters of severe zoonoses including plague and Lyme borreliosis. However, parasites are generally distributed in a nonrandom way. Exploring the distribution patterns of parasites and the mechanisms behind the patterns should contribute to a comprehensive understanding of ecosystem functioning, as well as disease control, human well-being, animal husbandry, and wildlife management [ 4 , 5 , 6 , 7 ].

A nearly universal pattern in parasitology is that parasites usually show an aggregated distribution [ 8 ]. This means that host individuals are often significantly different in their encounter rate or susceptibility to parasites, resulting in a highly variable parasite load among host individuals. Several biological factors of hosts have been found associated with the parasite load, such as body size [ 9 , 10 ], personality [ 11 ], and sex [ 12 ]. For example, sex-biased parasitism has been frequently recorded and males are often more heavily infested than females [ 13 , 14 ]. Several mechanisms have been proposed to explain this pattern. For example, males are usually larger and have a larger home area than females, making them more likely to encounter more parasites or become the preferred targets of parasites. However, male-biased parasitism is not universal and seems to partially depend on parasite types examined [ 15 , 16 ].

Another interesting mechanism associated with the male-biased parasitism is the immunocompetence handicap hypothesis (ICHH), which states that a higher level of testosterone can promote the production of male secondary traits, but at the cost of decreased immune function [ 17 , 18 ]. Meanwhile, there is a negative-feedback loop between parasite load and the signal intensity of male secondary trait, as parasitism may hinder the development of several male secondary traits of hosts [ 19 ]. As a result, the enhanced expression of male secondary trait may act as an honest signal of “good genes,” i.e., an individual’s ability to withstand a higher parasite burden [ 17 ].

A fundamental prediction of the ICHH is that individuals with a higher level of testosterone should also possess a higher parasite load. This prediction has been experimentally tested in many species since the ICHH was proposed. However, the results are largely inconsistent [ 20 ], as positive [ 21 , 22 , 23 ], negative [ 24 , 25 ], and non-significant [ 26 , 27 , 28 ] association between testosterone level and parasite load have all been repeatedly recorded. The relationship between testosterone level and parasitism seems to be complex and may differ significantly among both host [ 20 ] and parasite species [ 24 , 29 ]. Compared with correlational studies, manipulative experiments should be more effective in detecting the actual role of testosterone level on parasite load.

Moreover, partly because the ICHH was developed to explain the expression of male secondary traits interacted with the immune function and endocrine system, no experimental study has explored the role of testosterone level in shaping the parasite load in females. However, testosterone is not exclusively limited to males, and females may also face a physiological tradeoff associated with testosterone [ 30 , 31 ]. The association between testosterone level and parasite load in females is poorly known and deserves more investigations [ 30 ].

In this study, we explored the effects of testosterone level on the flea and tick load in Daurian ground squirrel ( Spermophilus dauricus ) by manipulating the testosterone level of both male and females. S. dauricus is a medium-sized, diurnal rodent widely distributed in grasslands of northeastern Asia [ 32 ]. As a well-known host of fleas and ticks, S. dauricus often acts as an important transmitter of plague [ 13 , 33 ]. In a previous study, we found that fleas and ticks possessed distinct distribution patterns on S. dauricus , suggesting that the underlying mechanisms of parasitism may also differ between these two types of ectoparasites [ 13 ]. For this study, we aimed to address two main questions: (1) would the effects of testosterone level on parasite load differ between fleas and ticks? And, (2) would the effects of testosterone level on parasite load also differ between male and female ground squirrels?

We carried out our field work in the grassland located within the Experiment Demonstration Base, Grassland Research Institute, Chinese Academy of Agricultural Sciences (40° 36ʹ N, 111° 45ʹ E). This area has a continental temperate monsoon climate, with an average annual rainfall of ca. 400 mm and an annual mean temperature of ca. 6.9 °C. The dominant plant species are Leymus chinensis , Stipa capillata , Cleistogenes squarrosa and Medicago sativa . Based on our own trapping record, S. dauricus has been the dominant rodent species here in recent years. Other rodents, such as striped hamster ( Cricetulus barabensis ) and Mongolian gerbil ( Meriones Unguiculatus ), were also recorded but relatively low in abundance. According to our observation, steppe polecats ( Mustela eversmanii ), red foxes ( Vulpes vulpes ) and domestic dogs ( Canis lupus familiaris ) are the major predators feeding on S. dauricus [ 32 ]. Cattle grazing is common here in spring and summer, resulting in an average grass height of ca. 20 cm and an average vegetation cover of 45%.

Experimental procedure

In mid-July, 2023, we conducted the first round of live-trapping in two 1-ha sites located in the study area. In this season, most S. dauricus were reproductively inactive. The two sites were comparable in terms of vegetation, topography and rodent density. To ensure independence in sampling among the sites, there was a distance of 400 m between the nearest sites. We placed 100 Sherman live traps (arranged in a 10 × 10 grid, with 10-m intervals between neighboring traps) baited with fresh peanuts in each site. Since S. dauricus were diurnal, the traps were set open between 07:00 and 19:00 (Beijing time). This round of live-trapping lasted for four consecutive days. We checked all the traps every 2 h and rebaited the traps if needed. All the S. dauricus captured were immediately put in separate cotton bags and taken back to our laboratory.

A total of 59 S. dauricus (27 males and 32 females) were captured during the first round of live-trapping. We anesthetized each individual by a multi-channel anesthesia machine designed for small animals (R550IE, RWD Life Science Co., Ltd., Shenzhen, China) with isoflurane. To collect the ectoparasites, the body surface of each S. dauricus was carefully scanned using a fine-toothed comb and a tweezer. We also checked the inner side of each cotton bag used to contain the ground squirrels. All the ectoparasites collected from a S. dauricus were immediately placed in ethanol (95%) contained within a separate 5-ml centrifuge tube. All the S. dauricus were weighted to the nearest 0.1 g using an electronic balance, toe-clipped for individual identification, and then maintained in separate plastic boxes for 72 h with access to ad libitum food (peanuts, alfalfa leaves, and commercial pellets) and water. No S. dauricus showed any abnormal behavior or healthy problem during this period.

We used all the 52 adult individuals (defined as those heavier than 100 g, 23 males and 29 females) for our formal experiment. Each ground squirrel was randomly assigned to one of two groups: control group (without testosterone injection, 11 males and 14 females) and treatment group (with testosterone injection, 12 males and 15 females). At 15:00–16:00 in the next day after capture, we collected a fresh fecal sample (typically 0.2–0.3 g) from each experimental animal. All the fecal samples were placed in separate 5-ml centrifuge tubes and then immediately stored frozen at −80 °C. About 72 h after capture, each individual was injected intramuscularly with either a dose of tea oil (control group) or a dose of testosterone–oil mixture (10 mg of testosterone undecanoate per ml of tea oil). A total of 1 h after injection, we released all the individuals at the places where they were captured.

A total of 10 days later, we conducted the second round (five consecutive days) of live trapping to recapture the experimental animals. The procedures of live-trapping, anesthesia, ectoparasite collection, fecal sample collection, and animal maintenance were similar to the first round. A total of 28 S. dauricus were recaptured (six males and seven females from the control group, and five males and ten females from the treatment group). An experienced taxonomist (Jian-Jun Wang) later identified all the ectoparasites based on dichotomous keys. The whole experimental procedure adhered to the guidelines approved by the American Society of Mammalogists [ 34 ] and the Regulations of the Animal Welfare Committee of Beijing Veterinarians of the Agriculture Ministry of China (Beijing, China).

Hormone extraction and analyses

We typically followed the protocol used by Li et al. [ 35 ] to extract testosterone from the fecal samples, with some modifications. A total of 56 fecal samples were used for hormone analyses (i.e., samples collected from the 28 individuals with recaptures, two samples per individual). Since we used wet feces, variations in water content among samples must be accounted for. Therefore, we simultaneously weighed two fecal subsamples (each ca. 0.1 g in weight, hereafter subsamples A and B) from each fecal sample. Subsample B was used for measuring water content and was weighed before and after 24-h drying in a drying oven. The water content value was then used to translate the wet sample weight of the relevant subsample A into dry weight.

Subsamples A were used for hormone extraction and placed in separate 10-ml centrifuge tubes. For each tube, we added 4 ml of methanol and 1 ml of distilled water and then vortexed it for 30 min. We then added 2.5 ml of petroleum ether to each tube to remove lipid from it. After 10 min of vortex, each tube was centrifuged at 1500 r/min for 15 min. A total of 2 ml of liquid was drawn from the methanol layer within each tube and then placed into a 5-ml cryopreservation tube. The methanol was dried off under forced air and the remain was used for hormone assay.

We performed testosterone assays with a commercially available enzyme immunoassay kit (Rat Testosterone Elisa Kit, produced by FanYin Biotechnology Co., Ltd., Shanghai, China). This kit has a sensitivity of 1.0 nanomol/l, and < 1% cross-reactivity to other steroids (including progestins, corticoids and estrogens). The testosterone levels were reported as nanogram of fecal testosterone per gram of dry feces.

Statistical analyses

We performed all the statistical work in R platform 4.2.2 [ 36 ]. We first adopted a paired-sample t -test to test whether our experimental treatment affected the fecal testosterone level of S. dauricus . We built a negative-binomial generalized linear mixed-effect model (GLMM) on tick load recorded on the recaptured individuals (hereafter TickLoad after ) using the R package “lme4” [ 37 ] and “lmerTest” [ 38 ]. The fixed terms included treatment (control or treatment group), sex (male or female), body weight (averaged value of the two measurements), tick load recorded in the first round of ectoparasite check (i.e., tick load before the testosterone manipulation, hereafter TickLoad before ), flea load in the second round of ectoparasite check (hereafter FleaLoad after ), and an interactive term between treatment and sex. Site ID was used as a random term. Similar models were also built for FleaLoad after , with fixed factors including treatment, sex, body weight, flea load recorded in the first round of ectoparasite check (hereafter FleaLoad before ), TickLoad after , and an interaction between treatment and sex. Variance inflation factors (VIFs) were calculated using the R package ‘car’ [ 39 ] to assess multicollinearity. As the VIFs were all smaller than ten (Table 1 ), we retained all the factors in the models. Model selection was performed based on Alkaike Information Criterion corrected for small sample size (AIC c ) [ 40 ] using the R package “MuMIn” [ 41 ]. Since the performance did not differ significantly between top candidate models (i.e., delta AIC c smaller than 2), we used conditional model averaging to get an “averaged model” based on the full set of candidate models [ 40 ]. As we detected a significant interactive effect between treatment and sex on TickLoad after , we also built two GLMMs on TickLoad after for male and female squirrels separately (Table 2 ). For these two models, the fixed terms included treatment, body weight, TickLoad before , and FleaLoad after .

On the 28 squirrels used for analyses, we collected a total of 534 ticks and 73 fleas before testosterone manipulation, and 1108 ticks and 132 fleas in the second round of ectoparasite check (Additional file 1 ). Before testosterone manipulation, no significant difference in tick load was detected between males and females (Wilcoxon test: W = 79, P = 0.50). After testosterone manipulation, males were more heavily infested by ticks than females (Wilcoxon test: W = 47.5, P = 0.033). The diversity of ectoparasites was rather low, as only one tick species ( Haemaphysalis verticalis ) and one flea species ( Citellophilus tesquorum mongolicus ) were recorded. The prevalence of fleas was 57.14% (16/28) and 64.29% (18/28) in the first and the second round of ectoparasite check, respectively. The prevalence of ticks was 67.86% (19/28) and 100% in the first and the second round of ectoparasite check, respectively.

Compared to the testosterone level prior to the experimental manipulation, the squirrels subjected to testosterone injection showed a significantly increased testosterone level after the manipulation (Wilcoxon test: W = 198, P < 0.001), while those subjected to tea oil injection did not show such a change in testosterone level (Wilcoxon test: W = 98, P = 0.50). According to the averaged GLMM, sex and treatment significantly interacted to affect TickLoad after (Table 1 , Fig. 1 ). We also detected a marginally positive association between flea load and tick load after testosterone manipulation (Table 1 ). However, no significant association was found between the explanatory factors and FleaLoad after . For male squirrels, individuals subjected to testosterone injection possessed a significantly higher TickLoad after (Table 2 ). However, such a trend did not exist in female squirrels (Table 2 ).

Boxplots indicating the interactive effects of sex (male/female) and treatment (testosterone/control) on tick load and flea load in Daurian ground squirrels ( Spermophilus dauricus )

In consistence with the ICHH, we found that male squirrels subjected to testosterone implantation later possessed a significantly higher tick load than those from the control group. Several mechanisms may contribute to this pattern. First, increased androgens often make animals more active and more aggressive [ 42 ], thus increasing their chance of encountering other individuals, as well as their exposure to parasites transmitted by conspecifics. Second, as the ICHH states, while increased testosterone level helps to develop male secondary traits and bring some reproductive benefits, it may also cause increased host susceptibility to parasites through immunosuppression [ 43 ]. Of course, these mechanisms are not mutually exclusive. To further disentangle the roles of behavioral changes and immunosuppression in shaping parasite abundance, it is important to simultaneously monitor behavioral pattern and immune functions of hosts, which is the aim of our next-step work.

As documented before, male-biased parasitism is not a universal pattern. This is also the case for the association between testosterone level and parasite infestation. In this study, testosterone treatment did not seem to significantly affect flea load in S. dauricus . In our previous work, we detected no sex difference in flea load in this rodent [ 13 ]. Similarly, Kowalski et al. [ 44 ] also found that flea load did not differ significantly between male and female yellow-necked field mice ( Apodemus flavicollis ). Difference in behavior and life history between ticks and fleas may partly explain their difference in parasitism. Compared with ticks, fleas are generally more mobile, more sensitive to environmental changes and need to lay eggs in the host burrows. As many other mammals do, female adult S. dauricus solitarily nurse their offsprings. Meanwhile, unlike males, female S. dauricus usually built their nests with relatively soft and fine bedding materials, which may help them to maintain the ambient temperature within the nests [ 45 ]. As a result, the burrow of a female adult S. dauricus with its cubs should be more attractive for fleas than a male’s burrow [ 46 ]. Moreover, living with cubs may also promote transmission of fleas between mother and cubs [ 47 ]. These mechanisms may thus counterbalance the male-biased pattern or the positive effect of testosterone level on parasitism.

Although the androgen-mediated trade-off between reproductive success and health has been repeatedly reported in males, it was much less studied in females. In a previous work on female meerkats, Smyth et al. [ 30 ] found a positive association between fecal testosterone level and the abundance of nematodes. This is not the case in our study, as we detected no significant effect of testosterone manipulation on ectoparasite load in female squirrels. Two reasons may contribute to this difference: first, unlike female meerkats, female S. dauricus are solitary and may not rely on increased androgen level to maintain its dominance status or stay in a central position within its social network. As a result, the increased testosterone level should be less effective to facilitate the female squirrels’ exposure to parasites. Second, the study on female meerkats is by essence a natural experiment and based on correlational analyses, which is unable to reveal the exact causal relationship between testosterone level and parasite load, especially when changes in parasite load also trigger changes in testosterone level.

The ICHH has long been used to explain the widespread sexual differences in parasite load. However, this hypothesis remains controversial to some extent [ 20 ], partly because one of its fundamental prediction—the positive relationship between testosterone level and parasite load- is not consistently supported by empirical studies. In this study, we tested this prediction by manipulating testosterone level in a medium-sized rodent. We found that testosterone supplementation had a positive effect on tick load in males, but not in females. Moreover, testosterone manipulation did not significantly affect flea load in S. dauricus . In summary, our results suggested that the role of testosterone on regulating parasite load is relatively complex, and may largely depend on parasite type and gender of hosts. The lack of generality in the testosterone effect is reasonable to some extent, as testosterone can shape parasite load in multiple ways, such as changing encounter rate of parasites through behavioral alteration, and modulating resistance to parasites through many physiological processes (e.g., impairing antibody production, and interacting with corticosteroids), which are not always immunosuppressive. These mechanisms can take effect together, and the overall effect of testosterone should depend on biological traits of both hosts and parasites. It is thus important to simultaneously track testosterone-related changes in behavioral mode (e.g., social behavior, home range, and activity level) and physiological status (e.g., number of antibodies, number of white blood cells, and glucocorticoids level) of both male and female hosts, if we intend to disentangle the roles of various mechanisms in regulating parasite load. An up-to-date meta-analysis or global synthesis is also required to grasp the global trend of testosterone effect, and to explain the heterogeneity existing among the various studies.

Availability of data and materials

The dataset used for analyses in this study can be found in the supplementary information.

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Acknowledgements

We are thankful to Yu-Chuang Hui, Yong-Wang Guo, Meng-Yuan Xu and Zhu-Qin Long for their generous help in our field work.

This study received financial support from the National Natural Science Foundation of China (grants 32172434 and 32172437), the Joint Funds of the Natural Science Foundation of Inner Mongolia (2023LHMS03036), Central Guiding Local Science and Technology Development Fund Projects (grant 2022ZY0195), Inner Mongolia Autonomous Region Science and Technology Plan Project (grant 2022YFDZ0064), and Natural Science Research Project for Anhui Universities (grant 2023AH050330).

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Li-Qing Wang, Zhi-Tao Liu and Jian-Jun Wang contributed equally to this work.

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Grassland Research Institute, Chinese Academy of Agricultural Sciences, Hohhot, China

Li-Qing Wang & Fu-Shun Zhang

College of Life Sciences, Harbin Normal University, Harbin, China

Zhi-Tao Liu

Inner Mongolia Autonomous Region Comprehensive Center for Disease Control and Prevention, Hohhot, China

Jian-Jun Wang

College of Life Sciences, Huaibei Normal University, Huaibei, China

Yu-Han Fang, Hao Zhu, Ke Shi & Ling-Ying Shuai

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LQW, ZTL, and LYS conceived and designed the study, LQW and LYS wrote the paper, ZTL, JJW, YHF, HZ, and KS performed the experiments and analyzed the data. FSZ contributed to study design and edited the manuscript. All authors read and approved the final manuscript.

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Correspondence to Ling-Ying Shuai .

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Additional file 1: table s1..

Original data used for analyses in this study.

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Wang, LQ., Liu, ZT., Wang, JJ. et al. Complex effects of testosterone level on ectoparasite load in a ground squirrel: an experimental test for the immunocompetence handicap hypothesis. Parasites Vectors 17 , 164 (2024). https://doi.org/10.1186/s13071-024-06261-1

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Ch. 9 Flashcards
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Complex effects of testosterone level on ectoparasite load in a ground
The immunocompetence handicap hypothesis suggests that males with a higher testosterone level should be better at developing male secondary traits, but at a cost of suppressed immune performance. As a result, we should expect that males with an increased testosterone level also possess a higher parasite load. However, previous empirical studies aimed to test this prediction have generated ...

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Hypothesis Testing | A Step-by-Step Guide with Easy Examples

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Rebecca Bevans

7.1: Basics of Hypothesis Testing

Hypothesis Testing

What is Hypothesis Testing in Statistics?

Hypothesis Testing Definition

Null Hypothesis

Alternative Hypothesis

Hypothesis Testing P Value

Hypothesis Testing Critical region

Hypothesis Testing Formula

Types of Hypothesis Testing

Hypothesis Testing Z Test

Hypothesis Testing t Test

Hypothesis Testing Chi Square

One Tailed Hypothesis Testing

Two Tailed Hypothesis Testing

Hypothesis Testing Steps

Hypothesis Testing Example

Hypothesis Testing and Confidence Intervals

Examples on Hypothesis Testing

FAQs on Hypothesis Testing

What is the z Test in Hypothesis Testing?

What is the t Test in Hypothesis Testing?

What is the formula for z test in Hypothesis Testing?

What is the p Value in Hypothesis Testing?

What is One Tail Hypothesis Testing?

What is the Alpha Level in Two Tail Hypothesis Testing?

17 Introduction to Hypothesis Testing

What is Hypothesis Testing?

Steps of Hypothesis Testing

Regions of the Distribution

Factors that Influence and Assumptions of Hypothesis Testing

Directional Hypotheses and Tailed Tests

Effect Size

Statistical Power

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Introduction to Hypothesis Testing

The Two Types of Statistical Hypotheses

Hypothesis Tests

The Two Types of Decision Errors

One-Tailed and Two-Tailed Tests

Types of Hypothesis Tests

Published by Zach

Hypothesis Testing

Definitions and Methodology

Unit 12: Significance tests (hypothesis testing)

Error probabilities and power

Tests about a population proportion

Tests about a population mean

More significance testing videos

What is a Hypothesis?

What are the Types of Hypotheses?

2. Complex Hypothesis

3. Null Hypothesis

4. Alternative Hypothesis

5. Logical Hypothesis

6. Empirical Hypothesis

7. Statistical Hypothesis

What is Hypothesis Testing?

How Hypothesis Testing Works

What Are The Stages of Hypothesis Testing?

Applications of Hypothesis Testing in Research

What is an Example of Hypothesis Testing?

Importance/Benefits of Hypothesis Testing

Criticism and Limitations of Hypothesis Testing

Type I vs Type II Errors: Causes, Examples & Prevention

Internal Validity in Research: Definition, Threats, Examples

What is Pure or Basic Research? + [Examples & Method]

Alternative vs Null Hypothesis: Pros, Cons, Uses & Examples

Formplus - For Seamless Data Collection

Tutorial Playlist

The Best Guide to Understand Bayes Theorem

A Complete Guide to Get a Grasp of Time Series Analysis