In Vitro Antimicrobial Activity of Some Medicinal Plants against Human Pathogenic Bacteria
Affiliation.
- 1 Tri-Chandra Multiple College, Tribhuvan University, Kathmandu, Nepal.
- PMID: 31065287
- PMCID: PMC6466868
- DOI: 10.1155/2019/1895340
The emergence and spread of antibiotic resistance, as well as the evolution of new strains of disease causing agents, are of great concern to the global health community. Effective treatment of a disease entails the development of new pharmaceuticals or some potential source of novel drugs. Commonly used medicinal plants of our community could be an excellent source of drugs to fight off this problem. This study is focused on exploring the antimicrobial properties of the plants that are commonly being used as traditional medicines. The antimicrobial potential of four different plant extracts was screened against twelve pathogenic microorganisms and two reference bacterial strains. Methanolic extracts of Oxalis corniculata , Artemisia vulgaris , Cinnamomum tamala , and Ageratina adenophora were subjected to a test of their antimicrobial properties by agar well diffusion method. The result indicated that most of the extracts exhibited antimicrobial properties. The highest potential was observed in the extract of O. corniculata against Escherichia coli, Salmonella Typhi, MDR Salmonella Typhi, Klebsiella pneumoniae, and Citrobacter koseri with zone of inhibition (ZOI) of 17 mm, 13 mm, 16 mm, 11 mm, and 12 mm, respectively. Oxalis corniculata also showed the highest MIC against test organisms. The methanolic extract of Artemisia vulgaris , Cinnamomum tamala, and Ageratina adenophora showed efficacy against Staphylococcus aureus. Ageratina adenophora also showed antifungal activity against Rhizopus spp. The experiment confirmed the efficacy of some selected plant extracts as natural antimicrobials and suggested the possibility of employing them in drugs for the treatment of infectious diseases caused by the test organisms.
ORIGINAL RESEARCH article
Next generation sequencing-aided screening, isolation, molecular identification, and antimicrobial potential for bacterial endophytes from the medicinal plant, elephantorrhiza elephantina.
- 1 North-West University, Mafikeng, South Africa
- 2 University of Venda, Thohoyandou, Limpopo, South Africa
- 3 University of Johannesburg, Johannesburg, Gauteng, South Africa
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Elephantorrhiza elephantina, a wild plant in southern Africa, is utilized in traditional medicine for various ailments, leading to its endangerment and listing on the Red List of South African Plants. To date, there have been no reports on bacterial endophytes from this plant, their classes of secondary metabolites, and potential medicinal properties. This study presents (i) taxonomic characterization of bacterial endophytes in leaf and root tissues using 16S rRNA, (ii) bacterial isolation, morphological, and phylogenetic characterization, (iii) bacterial growth, metabolite extraction, and LC-MS-based metabolite fingerprinting, and (iv) antimicrobial testing of bacterial crude extracts. Next-generation sequencing yielded 693 and 2459 DNA read counts for the rhizomes and leaves, respectively, detecting phyla including Proteobacteria,
Keywords: Elephantorriza elephantina, Next-generation sequencing, Taxonomy, bacterial endophytes, phylogeny, secondary metabolites, Antimicrobial activity
Received: 08 Feb 2024; Accepted: 06 May 2024.
Copyright: © 2024 Tlou, Ndou, Mabona, Khwathisi, Ateba, Madala and Serepa-Dlamini. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY) . The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Matsobane Tlou, North-West University, Mafikeng, South Africa
Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
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that in aqueous extr act. 9 Rai observed. antimycotic activit y against the t est pathogen. Pe stalotiopsis mangiferae in 14 medicinal. plants. Maximum anti -mycotic activity was. shown by ...
2. Antimicrobial Activity of Medicinal Plant Extracts. Extracts isolated from medicinal plants have been reported exhibit various biological activities such as antimicrobial, anti-inflammatory, and antioxidant activities [].The antimicrobial compounds from medicinal plants may inhibit the growth of bacteria, fungi, viruses, and protozoa by different mechanisms than those of presently used ...
Overall, many natural products have antibacterial activity when used as a single agent, and may act synergistically in combination with another plant products, or can help to increase the antimicrobial effect of conventional antimicrobial drugs [196,199,204]. The rationale of combination therapy is also supported by the recommendations of the ...
The success story of artemisinin serves as the best example for encouraging ethnobotanists to pursue research on more plant derived ... J.G., Ostrowsky B., Rex J.H. White paper: Developing antimicrobial drugs for resistant pathogens, narrow-spectrum indications, and unmet needs. ... L. Evidence of synergistic activity of medicinal plant ...
In a first study of ongoing research, endophytic bacteria were isolated from two medicinal plants, Hypericum perforatum and Ziziphora capitata with contrasting antimicrobial activities from the Chatkal Biosphere Reserve of Uzbekistan, and their plant-specific traits involved in biocontrol and plant growth promotion were evaluated.
The focus is put on the current challenges and future perspectives surrounding medicinal plants antimicrobial activity. There are some inherent challenges regarding medicinal plant extracts and their antimicrobial efficacy. ... while efforts have been made to enhance the antimicrobial activity of chemical compounds. Research on the mechanisms ...
Plants produce a variety of bioactive secondary metabolites that could be used to fuel the future discovery pipeline. While many studies have focused on specific aspects of plants and plant natural products with antibacterial properties, a comprehensive review of the antibacterial potential of plants has never before been attempted.
In the present paper, we analyze the past, present and future of medicinal plants, both as potential antimicrobial crude drugs as well as a source for natural compounds that act as new anti-infection agents. In the past few decades, the search for new anti-infection agents has occupied many research groups in the field of ethnopharmacology.
This review explores the antimicrobial activity of plant-derived components, their possible mechanisms of action, as well as their chemical potential, to identify and isolate new bioactive compounds from medicinal plants using standardized modern analytical procedures. The increasing incidence of drug- resistant pathogens raises an urgent need to identify and isolate new bioactive compounds ...
2.1. Plant material. In the investigation, the whole plant of Guava (Psidium guajava), Sage (Salvia officinalis), Rhamnus (Ziziphusspina Christi), Mulberry (Morusalba L.), and olive (Olea europaea L) leaves were collected from field market of Egypt.The plants are air dried in an oven at 40 °C for 48 h. Two hundred and fifty grams of dried powdered plant sample was extracted by one liter of ...
This study supports previous findings in the literature that the antimicrobial activities have a direct relation to increasing the extracts concentration (%) (Bhalodia and Shukla, 2011).Significant antimicrobial effects, expressed as MIC of each plant extract against test microorganism is given in Table 3.The data revealed variability in the MIC among plant extracts, the lowest MIC values (0. ...
Historically, plants have provided a source of the development for novel drugs and plant derived drugs which have made large contributions to human health and well being, but the therapeutic ability of number of plants are still unknown. Medicinal plant 'Rasayana' has played a vital role in the rejuvenation of worse condition created by the infection of microorganisms. Moreover, medicinal ...
The antimicrobial activity of the aqueous and ethanolic extracts of Myrtus communis, Ammi visnaga, and Equisetum arvense was investigated against Staphylococcus aureus, Escherichia coli, and Candida albicans by using the agar well diffusion method. Serial concentrations (15%, 30%, and 50%) of the extracts of each plant were tested and compared with gentamicin (10 μg) and fluconazole (25 μg).
This study is focused on exploring the antimicrobial properties of the plants that are commonly being used as traditional medicines. The antimicrobial potential of four different plant extracts was screened against twelve pathogenic microorganisms and two reference bacterial strains. Methanolic extracts of Oxalis corniculata, Artemisia vulgaris ...
Screening of antibacterial activity. All the bacterial isolates were checked and identified on the basis of conventional methods for purity and maintained on nutrient agar at 4°C in the refrigerator for further work. Antibacterial activity of compounds on the test organisms were determined by using agar-well method.
Considering the vast potentiality of plants as sources for antimicrobial drugs, this study aimed to investigate in vitro antibacterial and antifungal activity of extracts from some selected medicinal plants from Nepal against the most common microbial pathogens including MDR bacteria. Go to: 2. Materials and Methods.
Antifungal and antibacterial properties of glycosides were also informed. Phenols and flavonoids possess antioxidant, anti - allergic and antibacterial properties. Saponins possess anti - inflammatory, antiviral and plant defence activities [1]. Figure 1:Medicinal properties of phytochemicals.
Antimicrobial resistant poultry pathogens are responsible for treatment failure and economic losses, and can also be a source of resistant zoonotic infections representing a risk to human health. In 2006 the European Union banned the use of antibiotics as growth promoters in farm animals and other regions are likely to follow suit. Alternative products and strategies are sought to help ...
Introduction. Antimicrobial resistance (AMR) threatens the ability to successfully treat infectious diseases across the globe (McEwen and Collignon, 2018).While many factors such as the unnecessary prescription of antimicrobials and their use in agriculture contribute to the spread of AMR, a combination of scientific and economic challenges in antimicrobial development has caused the pipeline ...
In the recent years, research on medicinal plants has attracted a lot of attentions globally and large body of evidence has accumulated to demonstrate the promising potential of Medicinal Plants used in various traditional, complementary and alternate systems of treatment of human diseases. In the recent years, research on medicinal plants has attracted a lot of attentions globally. Large body ...
Ethyl acetate extract showing the moderate activity, and methanol extract and 50%methanol extract showing the tested microbes. 4. CONCLUSION. In the present study of investigation, different extracts of above plants were evaluated for their antibacterial activity against certain bacteria, and fungus.
This article is part of the Research Topic Plant Microbiome: Interactions, Mechanisms of Action, and Applications, Volume III View all 7 articles Next generation sequencing-aided screening, isolation, molecular identification, and antimicrobial potential for bacterial endophytes from the medicinal plant, Elephantorrhiza elephantina
The aim of this study was to evaluate the antimicrobial activity of some medicinal plant used in Ayurveda and traditional medicinal system for treatment of manifestations caused by microorganisms. ... Antimicrobial activity of plant extract was considered to be good if its MIC was less than 100.0 µg/ml, moderate if MIC was from 100.0 to 500.0 ...