nature hypothesis examples

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Biology library

Course: biology library   >   unit 1, the scientific method.

• Controlled experiments
• The scientific method and experimental design

Introduction

• Make an observation.
• Ask a question.
• Form a hypothesis , or testable explanation.
• Make a prediction based on the hypothesis.
• Test the prediction.
• Iterate: use the results to make new hypotheses or predictions.

Scientific method example: Failure to toast

1. make an observation..

• Observation: the toaster won't toast.

2. Ask a question.

• Question: Why won't my toaster toast?

3. Propose a hypothesis.

• Hypothesis: Maybe the outlet is broken.

4. Make predictions.

• Prediction: If I plug the toaster into a different outlet, then it will toast the bread.

5. Test the predictions.

• Test of prediction: Plug the toaster into a different outlet and try again.
• If the toaster does toast, then the hypothesis is supported—likely correct.
• If the toaster doesn't toast, then the hypothesis is not supported—likely wrong.

Logical possibility

Practical possibility, building a body of evidence, 6. iterate..

• Iteration time!
• If the hypothesis was supported, we might do additional tests to confirm it, or revise it to be more specific. For instance, we might investigate why the outlet is broken.
• If the hypothesis was not supported, we would come up with a new hypothesis. For instance, the next hypothesis might be that there's a broken wire in the toaster.

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What is a scientific hypothesis?

It's the initial building block in the scientific method.

Hypothesis basics

What makes a hypothesis testable.

• Types of hypotheses
• Hypothesis versus theory

Additional resources

Bibliography.

A scientific hypothesis is a tentative, testable explanation for a phenomenon in the natural world. It's the initial building block in the scientific method . Many describe it as an "educated guess" based on prior knowledge and observation. While this is true, a hypothesis is more informed than a guess. While an "educated guess" suggests a random prediction based on a person's expertise, developing a hypothesis requires active observation and background research. 

The basic idea of a hypothesis is that there is no predetermined outcome. For a solution to be termed a scientific hypothesis, it has to be an idea that can be supported or refuted through carefully crafted experimentation or observation. This concept, called falsifiability and testability, was advanced in the mid-20th century by Austrian-British philosopher Karl Popper in his famous book "The Logic of Scientific Discovery" (Routledge, 1959).

A key function of a hypothesis is to derive predictions about the results of future experiments and then perform those experiments to see whether they support the predictions.

A hypothesis is usually written in the form of an if-then statement, which gives a possibility (if) and explains what may happen because of the possibility (then). The statement could also include "may," according to California State University, Bakersfield .

Here are some examples of hypothesis statements:

• If garlic repels fleas, then a dog that is given garlic every day will not get fleas.
• If sugar causes cavities, then people who eat a lot of candy may be more prone to cavities.
• If ultraviolet light can damage the eyes, then maybe this light can cause blindness.

A useful hypothesis should be testable and falsifiable. That means that it should be possible to prove it wrong. A theory that can't be proved wrong is nonscientific, according to Karl Popper's 1963 book " Conjectures and Refutations ."

An example of an untestable statement is, "Dogs are better than cats." That's because the definition of "better" is vague and subjective. However, an untestable statement can be reworded to make it testable. For example, the previous statement could be changed to this: "Owning a dog is associated with higher levels of physical fitness than owning a cat." With this statement, the researcher can take measures of physical fitness from dog and cat owners and compare the two.

Types of scientific hypotheses

In an experiment, researchers generally state their hypotheses in two ways. The null hypothesis predicts that there will be no relationship between the variables tested, or no difference between the experimental groups. The alternative hypothesis predicts the opposite: that there will be a difference between the experimental groups. This is usually the hypothesis scientists are most interested in, according to the University of Miami .

For example, a null hypothesis might state, "There will be no difference in the rate of muscle growth between people who take a protein supplement and people who don't." The alternative hypothesis would state, "There will be a difference in the rate of muscle growth between people who take a protein supplement and people who don't."

If the results of the experiment show a relationship between the variables, then the null hypothesis has been rejected in favor of the alternative hypothesis, according to the book " Research Methods in Psychology " (​​BCcampus, 2015). 

There are other ways to describe an alternative hypothesis. The alternative hypothesis above does not specify a direction of the effect, only that there will be a difference between the two groups. That type of prediction is called a two-tailed hypothesis. If a hypothesis specifies a certain direction — for example, that people who take a protein supplement will gain more muscle than people who don't — it is called a one-tailed hypothesis, according to William M. K. Trochim , a professor of Policy Analysis and Management at Cornell University.

Sometimes, errors take place during an experiment. These errors can happen in one of two ways. A type I error is when the null hypothesis is rejected when it is true. This is also known as a false positive. A type II error occurs when the null hypothesis is not rejected when it is false. This is also known as a false negative, according to the University of California, Berkeley . 

A hypothesis can be rejected or modified, but it can never be proved correct 100% of the time. For example, a scientist can form a hypothesis stating that if a certain type of tomato has a gene for red pigment, that type of tomato will be red. During research, the scientist then finds that each tomato of this type is red. Though the findings confirm the hypothesis, there may be a tomato of that type somewhere in the world that isn't red. Thus, the hypothesis is true, but it may not be true 100% of the time.

Scientific theory vs. scientific hypothesis

The best hypotheses are simple. They deal with a relatively narrow set of phenomena. But theories are broader; they generally combine multiple hypotheses into a general explanation for a wide range of phenomena, according to the University of California, Berkeley . For example, a hypothesis might state, "If animals adapt to suit their environments, then birds that live on islands with lots of seeds to eat will have differently shaped beaks than birds that live on islands with lots of insects to eat." After testing many hypotheses like these, Charles Darwin formulated an overarching theory: the theory of evolution by natural selection.

"Theories are the ways that we make sense of what we observe in the natural world," Tanner said. "Theories are structures of ideas that explain and interpret facts." 

• Read more about writing a hypothesis, from the American Medical Writers Association.
• Find out why a hypothesis isn't always necessary in science, from The American Biology Teacher.
• Learn about null and alternative hypotheses, from Prof. Essa on YouTube .

Encyclopedia Britannica. Scientific Hypothesis. Jan. 13, 2022. https://www.britannica.com/science/scientific-hypothesis

Karl Popper, "The Logic of Scientific Discovery," Routledge, 1959.

California State University, Bakersfield, "Formatting a testable hypothesis." https://www.csub.edu/~ddodenhoff/Bio100/Bio100sp04/formattingahypothesis.htm  

Karl Popper, "Conjectures and Refutations," Routledge, 1963.

Price, P., Jhangiani, R., & Chiang, I., "Research Methods of Psychology — 2nd Canadian Edition," BCcampus, 2015.‌

University of Miami, "The Scientific Method" http://www.bio.miami.edu/dana/161/evolution/161app1_scimethod.pdf  

William M.K. Trochim, "Research Methods Knowledge Base," https://conjointly.com/kb/hypotheses-explained/  

University of California, Berkeley, "Multiple Hypothesis Testing and False Discovery Rate" https://www.stat.berkeley.edu/~hhuang/STAT141/Lecture-FDR.pdf  

University of California, Berkeley, "Science at multiple levels" https://undsci.berkeley.edu/article/0_0_0/howscienceworks_19

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Module 1: Introduction to Biology

Experiments and hypotheses, learning outcomes.

• Form a hypothesis and use it to design a scientific experiment

Now we’ll focus on the methods of scientific inquiry. Science often involves making observations and developing hypotheses. Experiments and further observations are often used to test the hypotheses.

A scientific experiment is a carefully organized procedure in which the scientist intervenes in a system to change something, then observes the result of the change. Scientific inquiry often involves doing experiments, though not always. For example, a scientist studying the mating behaviors of ladybugs might begin with detailed observations of ladybugs mating in their natural habitats. While this research may not be experimental, it is scientific: it involves careful and verifiable observation of the natural world. The same scientist might then treat some of the ladybugs with a hormone hypothesized to trigger mating and observe whether these ladybugs mated sooner or more often than untreated ones. This would qualify as an experiment because the scientist is now making a change in the system and observing the effects.

Forming a Hypothesis

When conducting scientific experiments, researchers develop hypotheses to guide experimental design. A hypothesis is a suggested explanation that is both testable and falsifiable. You must be able to test your hypothesis through observations and research, and it must be possible to prove your hypothesis false.

For example, Michael observes that maple trees lose their leaves in the fall. He might then propose a possible explanation for this observation: “cold weather causes maple trees to lose their leaves in the fall.” This statement is testable. He could grow maple trees in a warm enclosed environment such as a greenhouse and see if their leaves still dropped in the fall. The hypothesis is also falsifiable. If the leaves still dropped in the warm environment, then clearly temperature was not the main factor in causing maple leaves to drop in autumn.

In the Try It below, you can practice recognizing scientific hypotheses. As you consider each statement, try to think as a scientist would: can I test this hypothesis with observations or experiments? Is the statement falsifiable? If the answer to either of these questions is “no,” the statement is not a valid scientific hypothesis.

Practice Questions

Determine whether each following statement is a scientific hypothesis.

Air pollution from automobile exhaust can trigger symptoms in people with asthma.

• No. This statement is not testable or falsifiable.
• No. This statement is not testable.
• No. This statement is not falsifiable.
• Yes. This statement is testable and falsifiable.

Natural disasters, such as tornadoes, are punishments for bad thoughts and behaviors.

a: No. This statement is not testable or falsifiable. “Bad thoughts and behaviors” are excessively vague and subjective variables that would be impossible to measure or agree upon in a reliable way. The statement might be “falsifiable” if you came up with a counterexample: a “wicked” place that was not punished by a natural disaster. But some would question whether the people in that place were really wicked, and others would continue to predict that a natural disaster was bound to strike that place at some point. There is no reason to suspect that people’s immoral behavior affects the weather unless you bring up the intervention of a supernatural being, making this idea even harder to test.

Testing a Vaccine

Let’s examine the scientific process by discussing an actual scientific experiment conducted by researchers at the University of Washington. These researchers investigated whether a vaccine may reduce the incidence of the human papillomavirus (HPV). The experimental process and results were published in an article titled, “ A controlled trial of a human papillomavirus type 16 vaccine .”

Preliminary observations made by the researchers who conducted the HPV experiment are listed below:

• Human papillomavirus (HPV) is the most common sexually transmitted virus in the United States.
• There are about 40 different types of HPV. A significant number of people that have HPV are unaware of it because many of these viruses cause no symptoms.
• Some types of HPV can cause cervical cancer.
• About 4,000 women a year die of cervical cancer in the United States.

Practice Question

Researchers have developed a potential vaccine against HPV and want to test it. What is the first testable hypothesis that the researchers should study?

• HPV causes cervical cancer.
• People should not have unprotected sex with many partners.
• People who get the vaccine will not get HPV.
• The HPV vaccine will protect people against cancer.

Experimental Design

You’ve successfully identified a hypothesis for the University of Washington’s study on HPV: People who get the HPV vaccine will not get HPV.

The next step is to design an experiment that will test this hypothesis. There are several important factors to consider when designing a scientific experiment. First, scientific experiments must have an experimental group. This is the group that receives the experimental treatment necessary to address the hypothesis.

The experimental group receives the vaccine, but how can we know if the vaccine made a difference? Many things may change HPV infection rates in a group of people over time. To clearly show that the vaccine was effective in helping the experimental group, we need to include in our study an otherwise similar control group that does not get the treatment. We can then compare the two groups and determine if the vaccine made a difference. The control group shows us what happens in the absence of the factor under study.

However, the control group cannot get “nothing.” Instead, the control group often receives a placebo. A placebo is a procedure that has no expected therapeutic effect—such as giving a person a sugar pill or a shot containing only plain saline solution with no drug. Scientific studies have shown that the “placebo effect” can alter experimental results because when individuals are told that they are or are not being treated, this knowledge can alter their actions or their emotions, which can then alter the results of the experiment.

Moreover, if the doctor knows which group a patient is in, this can also influence the results of the experiment. Without saying so directly, the doctor may show—through body language or other subtle cues—their views about whether the patient is likely to get well. These errors can then alter the patient’s experience and change the results of the experiment. Therefore, many clinical studies are “double blind.” In these studies, neither the doctor nor the patient knows which group the patient is in until all experimental results have been collected.

Both placebo treatments and double-blind procedures are designed to prevent bias. Bias is any systematic error that makes a particular experimental outcome more or less likely. Errors can happen in any experiment: people make mistakes in measurement, instruments fail, computer glitches can alter data. But most such errors are random and don’t favor one outcome over another. Patients’ belief in a treatment can make it more likely to appear to “work.” Placebos and double-blind procedures are used to level the playing field so that both groups of study subjects are treated equally and share similar beliefs about their treatment.

The scientists who are researching the effectiveness of the HPV vaccine will test their hypothesis by separating 2,392 young women into two groups: the control group and the experimental group. Answer the following questions about these two groups.

• This group is given a placebo.
• This group is deliberately infected with HPV.
• This group is given nothing.
• This group is given the HPV vaccine.
• a: This group is given a placebo. A placebo will be a shot, just like the HPV vaccine, but it will have no active ingredient. It may change peoples’ thinking or behavior to have such a shot given to them, but it will not stimulate the immune systems of the subjects in the same way as predicted for the vaccine itself.
• d: This group is given the HPV vaccine. The experimental group will receive the HPV vaccine and researchers will then be able to see if it works, when compared to the control group.

Experimental Variables

A variable is a characteristic of a subject (in this case, of a person in the study) that can vary over time or among individuals. Sometimes a variable takes the form of a category, such as male or female; often a variable can be measured precisely, such as body height. Ideally, only one variable is different between the control group and the experimental group in a scientific experiment. Otherwise, the researchers will not be able to determine which variable caused any differences seen in the results. For example, imagine that the people in the control group were, on average, much more sexually active than the people in the experimental group. If, at the end of the experiment, the control group had a higher rate of HPV infection, could you confidently determine why? Maybe the experimental subjects were protected by the vaccine, but maybe they were protected by their low level of sexual contact.

To avoid this situation, experimenters make sure that their subject groups are as similar as possible in all variables except for the variable that is being tested in the experiment. This variable, or factor, will be deliberately changed in the experimental group. The one variable that is different between the two groups is called the independent variable. An independent variable is known or hypothesized to cause some outcome. Imagine an educational researcher investigating the effectiveness of a new teaching strategy in a classroom. The experimental group receives the new teaching strategy, while the control group receives the traditional strategy. It is the teaching strategy that is the independent variable in this scenario. In an experiment, the independent variable is the variable that the scientist deliberately changes or imposes on the subjects.

Dependent variables are known or hypothesized consequences; they are the effects that result from changes or differences in an independent variable. In an experiment, the dependent variables are those that the scientist measures before, during, and particularly at the end of the experiment to see if they have changed as expected. The dependent variable must be stated so that it is clear how it will be observed or measured. Rather than comparing “learning” among students (which is a vague and difficult to measure concept), an educational researcher might choose to compare test scores, which are very specific and easy to measure.

In any real-world example, many, many variables MIGHT affect the outcome of an experiment, yet only one or a few independent variables can be tested. Other variables must be kept as similar as possible between the study groups and are called control variables . For our educational research example, if the control group consisted only of people between the ages of 18 and 20 and the experimental group contained people between the ages of 30 and 35, we would not know if it was the teaching strategy or the students’ ages that played a larger role in the results. To avoid this problem, a good study will be set up so that each group contains students with a similar age profile. In a well-designed educational research study, student age will be a controlled variable, along with other possibly important factors like gender, past educational achievement, and pre-existing knowledge of the subject area.

What is the independent variable in this experiment?

• Sex (all of the subjects will be female)
• Presence or absence of the HPV vaccine
• Presence or absence of HPV (the virus)

List three control variables other than age.

What is the dependent variable in this experiment?

• Sex (male or female)
• Rates of HPV infection
• Age (years)
• Revision and adaptation. Authored by : Shelli Carter and Lumen Learning. Provided by : Lumen Learning. License : CC BY-NC-SA: Attribution-NonCommercial-ShareAlike
• Scientific Inquiry. Provided by : Open Learning Initiative. Located at : https://oli.cmu.edu/jcourse/workbook/activity/page?context=434a5c2680020ca6017c03488572e0f8 . Project : Introduction to Biology (Open + Free). License : CC BY-NC-SA: Attribution-NonCommercial-ShareAlike

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How to Write a Great Hypothesis

Hypothesis Definition, Format, Examples, and Tips

Kendra Cherry, MS, is a psychosocial rehabilitation specialist, psychology educator, and author of the "Everything Psychology Book."

Amy Morin, LCSW, is a psychotherapist and international bestselling author. Her books, including "13 Things Mentally Strong People Don't Do," have been translated into more than 40 languages. Her TEDx talk,  "The Secret of Becoming Mentally Strong," is one of the most viewed talks of all time.

Verywell / Alex Dos Diaz

• The Scientific Method

Hypothesis Format

Falsifiability of a hypothesis.

• Operationalization

Hypothesis Types

Hypotheses examples.

• Collecting Data

A hypothesis is a tentative statement about the relationship between two or more variables. It is a specific, testable prediction about what you expect to happen in a study. It is a preliminary answer to your question that helps guide the research process.

Consider a study designed to examine the relationship between sleep deprivation and test performance. The hypothesis might be: "This study is designed to assess the hypothesis that sleep-deprived people will perform worse on a test than individuals who are not sleep-deprived."

At a Glance

A hypothesis is crucial to scientific research because it offers a clear direction for what the researchers are looking to find. This allows them to design experiments to test their predictions and add to our scientific knowledge about the world. This article explores how a hypothesis is used in psychology research, how to write a good hypothesis, and the different types of hypotheses you might use.

The Hypothesis in the Scientific Method

In the scientific method , whether it involves research in psychology, biology, or some other area, a hypothesis represents what the researchers think will happen in an experiment. The scientific method involves the following steps:

• Forming a question
• Performing background research
• Creating a hypothesis
• Designing an experiment
• Collecting data
• Analyzing the results
• Drawing conclusions
• Communicating the results

The hypothesis is a prediction, but it involves more than a guess. Most of the time, the hypothesis begins with a question which is then explored through background research. At this point, researchers then begin to develop a testable hypothesis.

Unless you are creating an exploratory study, your hypothesis should always explain what you  expect  to happen.

In a study exploring the effects of a particular drug, the hypothesis might be that researchers expect the drug to have some type of effect on the symptoms of a specific illness. In psychology, the hypothesis might focus on how a certain aspect of the environment might influence a particular behavior.

Remember, a hypothesis does not have to be correct. While the hypothesis predicts what the researchers expect to see, the goal of the research is to determine whether this guess is right or wrong. When conducting an experiment, researchers might explore numerous factors to determine which ones might contribute to the ultimate outcome.

In many cases, researchers may find that the results of an experiment  do not  support the original hypothesis. When writing up these results, the researchers might suggest other options that should be explored in future studies.

In many cases, researchers might draw a hypothesis from a specific theory or build on previous research. For example, prior research has shown that stress can impact the immune system. So a researcher might hypothesize: "People with high-stress levels will be more likely to contract a common cold after being exposed to the virus than people who have low-stress levels."

In other instances, researchers might look at commonly held beliefs or folk wisdom. "Birds of a feather flock together" is one example of folk adage that a psychologist might try to investigate. The researcher might pose a specific hypothesis that "People tend to select romantic partners who are similar to them in interests and educational level."

Elements of a Good Hypothesis

So how do you write a good hypothesis? When trying to come up with a hypothesis for your research or experiments, ask yourself the following questions:

• Is your hypothesis based on your research on a topic?
• Can your hypothesis be tested?
• Does your hypothesis include independent and dependent variables?

Before you come up with a specific hypothesis, spend some time doing background research. Once you have completed a literature review, start thinking about potential questions you still have. Pay attention to the discussion section in the  journal articles you read . Many authors will suggest questions that still need to be explored.

How to Formulate a Good Hypothesis

To form a hypothesis, you should take these steps:

• Collect as many observations about a topic or problem as you can.
• Evaluate these observations and look for possible causes of the problem.
• Create a list of possible explanations that you might want to explore.
• After you have developed some possible hypotheses, think of ways that you could confirm or disprove each hypothesis through experimentation. This is known as falsifiability.

In the scientific method ,  falsifiability is an important part of any valid hypothesis. In order to test a claim scientifically, it must be possible that the claim could be proven false.

Students sometimes confuse the idea of falsifiability with the idea that it means that something is false, which is not the case. What falsifiability means is that  if  something was false, then it is possible to demonstrate that it is false.

One of the hallmarks of pseudoscience is that it makes claims that cannot be refuted or proven false.

The Importance of Operational Definitions

A variable is a factor or element that can be changed and manipulated in ways that are observable and measurable. However, the researcher must also define how the variable will be manipulated and measured in the study.

Operational definitions are specific definitions for all relevant factors in a study. This process helps make vague or ambiguous concepts detailed and measurable.

For example, a researcher might operationally define the variable " test anxiety " as the results of a self-report measure of anxiety experienced during an exam. A "study habits" variable might be defined by the amount of studying that actually occurs as measured by time.

These precise descriptions are important because many things can be measured in various ways. Clearly defining these variables and how they are measured helps ensure that other researchers can replicate your results.

Replicability

One of the basic principles of any type of scientific research is that the results must be replicable.

Replication means repeating an experiment in the same way to produce the same results. By clearly detailing the specifics of how the variables were measured and manipulated, other researchers can better understand the results and repeat the study if needed.

Some variables are more difficult than others to define. For example, how would you operationally define a variable such as aggression ? For obvious ethical reasons, researchers cannot create a situation in which a person behaves aggressively toward others.

To measure this variable, the researcher must devise a measurement that assesses aggressive behavior without harming others. The researcher might utilize a simulated task to measure aggressiveness in this situation.

Hypothesis Checklist

• Does your hypothesis focus on something that you can actually test?
• Does your hypothesis include both an independent and dependent variable?
• Can you manipulate the variables?
• Can your hypothesis be tested without violating ethical standards?

The hypothesis you use will depend on what you are investigating and hoping to find. Some of the main types of hypotheses that you might use include:

• Simple hypothesis : This type of hypothesis suggests there is a relationship between one independent variable and one dependent variable.
• Complex hypothesis : This type suggests a relationship between three or more variables, such as two independent and dependent variables.
• Null hypothesis : This hypothesis suggests no relationship exists between two or more variables.
• Alternative hypothesis : This hypothesis states the opposite of the null hypothesis.
• Statistical hypothesis : This hypothesis uses statistical analysis to evaluate a representative population sample and then generalizes the findings to the larger group.
• Logical hypothesis : This hypothesis assumes a relationship between variables without collecting data or evidence.

A hypothesis often follows a basic format of "If {this happens} then {this will happen}." One way to structure your hypothesis is to describe what will happen to the  dependent variable  if you change the  independent variable .

The basic format might be: "If {these changes are made to a certain independent variable}, then we will observe {a change in a specific dependent variable}."

A few examples of simple hypotheses:

• "Students who eat breakfast will perform better on a math exam than students who do not eat breakfast."
• "Students who experience test anxiety before an English exam will get lower scores than students who do not experience test anxiety."​
• "Motorists who talk on the phone while driving will be more likely to make errors on a driving course than those who do not talk on the phone."
• "Children who receive a new reading intervention will have higher reading scores than students who do not receive the intervention."

Examples of a complex hypothesis include:

• "People with high-sugar diets and sedentary activity levels are more likely to develop depression."
• "Younger people who are regularly exposed to green, outdoor areas have better subjective well-being than older adults who have limited exposure to green spaces."

Examples of a null hypothesis include:

• "There is no difference in anxiety levels between people who take St. John's wort supplements and those who do not."
• "There is no difference in scores on a memory recall task between children and adults."
• "There is no difference in aggression levels between children who play first-person shooter games and those who do not."

Examples of an alternative hypothesis:

• "People who take St. John's wort supplements will have less anxiety than those who do not."
• "Adults will perform better on a memory task than children."
• "Children who play first-person shooter games will show higher levels of aggression than children who do not." 

Collecting Data on Your Hypothesis

Once a researcher has formed a testable hypothesis, the next step is to select a research design and start collecting data. The research method depends largely on exactly what they are studying. There are two basic types of research methods: descriptive research and experimental research.

Descriptive Research Methods

Descriptive research such as  case studies ,  naturalistic observations , and surveys are often used when  conducting an experiment is difficult or impossible. These methods are best used to describe different aspects of a behavior or psychological phenomenon.

Once a researcher has collected data using descriptive methods, a  correlational study  can examine how the variables are related. This research method might be used to investigate a hypothesis that is difficult to test experimentally.

Experimental Research Methods

Experimental methods  are used to demonstrate causal relationships between variables. In an experiment, the researcher systematically manipulates a variable of interest (known as the independent variable) and measures the effect on another variable (known as the dependent variable).

Unlike correlational studies, which can only be used to determine if there is a relationship between two variables, experimental methods can be used to determine the actual nature of the relationship—whether changes in one variable actually  cause  another to change.

The hypothesis is a critical part of any scientific exploration. It represents what researchers expect to find in a study or experiment. In situations where the hypothesis is unsupported by the research, the research still has value. Such research helps us better understand how different aspects of the natural world relate to one another. It also helps us develop new hypotheses that can then be tested in the future.

Thompson WH, Skau S. On the scope of scientific hypotheses .  R Soc Open Sci . 2023;10(8):230607. doi:10.1098/rsos.230607

Taran S, Adhikari NKJ, Fan E. Falsifiability in medicine: what clinicians can learn from Karl Popper [published correction appears in Intensive Care Med. 2021 Jun 17;:].  Intensive Care Med . 2021;47(9):1054-1056. doi:10.1007/s00134-021-06432-z

Eyler AA. Research Methods for Public Health . 1st ed. Springer Publishing Company; 2020. doi:10.1891/9780826182067.0004

Nosek BA, Errington TM. What is replication ?  PLoS Biol . 2020;18(3):e3000691. doi:10.1371/journal.pbio.3000691

Aggarwal R, Ranganathan P. Study designs: Part 2 - Descriptive studies .  Perspect Clin Res . 2019;10(1):34-36. doi:10.4103/picr.PICR_154_18

Nevid J. Psychology: Concepts and Applications. Wadworth, 2013.

By Kendra Cherry, MSEd Kendra Cherry, MS, is a psychosocial rehabilitation specialist, psychology educator, and author of the "Everything Psychology Book."

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• Knowledge Base
• Methodology
• How to Write a Strong Hypothesis | Guide & Examples

How to Write a Strong Hypothesis | Guide & Examples

Published on 6 May 2022 by Shona McCombes .

A hypothesis is a statement that can be tested by scientific research. If you want to test a relationship between two or more variables, you need to write hypotheses before you start your experiment or data collection.

Table of contents

What is a hypothesis, developing a hypothesis (with example), hypothesis examples, frequently asked questions about writing hypotheses.

A hypothesis states your predictions about what your research will find. It is a tentative answer to your research question that has not yet been tested. For some research projects, you might have to write several hypotheses that address different aspects of your research question.

A hypothesis is not just a guess – it should be based on existing theories and knowledge. It also has to be testable, which means you can support or refute it through scientific research methods (such as experiments, observations, and statistical analysis of data).

Variables in hypotheses

Hypotheses propose a relationship between two or more variables . An independent variable is something the researcher changes or controls. A dependent variable is something the researcher observes and measures.

In this example, the independent variable is exposure to the sun – the assumed cause . The dependent variable is the level of happiness – the assumed effect .

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Step 1: ask a question.

Writing a hypothesis begins with a research question that you want to answer. The question should be focused, specific, and researchable within the constraints of your project.

Step 2: Do some preliminary research

Your initial answer to the question should be based on what is already known about the topic. Look for theories and previous studies to help you form educated assumptions about what your research will find.

At this stage, you might construct a conceptual framework to identify which variables you will study and what you think the relationships are between them. Sometimes, you’ll have to operationalise more complex constructs.

Step 3: Formulate your hypothesis

Now you should have some idea of what you expect to find. Write your initial answer to the question in a clear, concise sentence.

Step 4: Refine your hypothesis

You need to make sure your hypothesis is specific and testable. There are various ways of phrasing a hypothesis, but all the terms you use should have clear definitions, and the hypothesis should contain:

• The relevant variables
• The specific group being studied
• The predicted outcome of the experiment or analysis

Step 5: Phrase your hypothesis in three ways

To identify the variables, you can write a simple prediction in if … then form. The first part of the sentence states the independent variable and the second part states the dependent variable.

In academic research, hypotheses are more commonly phrased in terms of correlations or effects, where you directly state the predicted relationship between variables.

If you are comparing two groups, the hypothesis can state what difference you expect to find between them.

Step 6. Write a null hypothesis

If your research involves statistical hypothesis testing , you will also have to write a null hypothesis. The null hypothesis is the default position that there is no association between the variables. The null hypothesis is written as H 0 , while the alternative hypothesis is H 1 or H a .

Hypothesis testing is a formal procedure for investigating our ideas about the world using statistics. It is used by scientists to test specific predictions, called hypotheses , by calculating how likely it is that a pattern or relationship between variables could have arisen by chance.

A hypothesis is not just a guess. It should be based on existing theories and knowledge. It also has to be testable, which means you can support or refute it through scientific research methods (such as experiments, observations, and statistical analysis of data).

A research hypothesis is your proposed answer to your research question. The research hypothesis usually includes an explanation (‘ x affects y because …’).

A statistical hypothesis, on the other hand, is a mathematical statement about a population parameter. Statistical hypotheses always come in pairs: the null and alternative hypotheses. In a well-designed study , the statistical hypotheses correspond logically to the research hypothesis.

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The Craft of Writing a Strong Hypothesis

Table of Contents

Writing a hypothesis is one of the essential elements of a scientific research paper. It needs to be to the point, clearly communicating what your research is trying to accomplish. A blurry, drawn-out, or complexly-structured hypothesis can confuse your readers. Or worse, the editor and peer reviewers.

A captivating hypothesis is not too intricate. This blog will take you through the process so that, by the end of it, you have a better idea of how to convey your research paper's intent in just one sentence.

What is a Hypothesis?

The first step in your scientific endeavor, a hypothesis, is a strong, concise statement that forms the basis of your research. It is not the same as a thesis statement , which is a brief summary of your research paper .

The sole purpose of a hypothesis is to predict your paper's findings, data, and conclusion. It comes from a place of curiosity and intuition . When you write a hypothesis, you're essentially making an educated guess based on scientific prejudices and evidence, which is further proven or disproven through the scientific method.

The reason for undertaking research is to observe a specific phenomenon. A hypothesis, therefore, lays out what the said phenomenon is. And it does so through two variables, an independent and dependent variable.

The independent variable is the cause behind the observation, while the dependent variable is the effect of the cause. A good example of this is “mixing red and blue forms purple.” In this hypothesis, mixing red and blue is the independent variable as you're combining the two colors at your own will. The formation of purple is the dependent variable as, in this case, it is conditional to the independent variable.

Different Types of Hypotheses‌

Types of hypotheses

Some would stand by the notion that there are only two types of hypotheses: a Null hypothesis and an Alternative hypothesis. While that may have some truth to it, it would be better to fully distinguish the most common forms as these terms come up so often, which might leave you out of context.

Apart from Null and Alternative, there are Complex, Simple, Directional, Non-Directional, Statistical, and Associative and casual hypotheses. They don't necessarily have to be exclusive, as one hypothesis can tick many boxes, but knowing the distinctions between them will make it easier for you to construct your own.

1. Null hypothesis

A null hypothesis proposes no relationship between two variables. Denoted by H 0 , it is a negative statement like “Attending physiotherapy sessions does not affect athletes' on-field performance.” Here, the author claims physiotherapy sessions have no effect on on-field performances. Even if there is, it's only a coincidence.

2. Alternative hypothesis

Considered to be the opposite of a null hypothesis, an alternative hypothesis is donated as H1 or Ha. It explicitly states that the dependent variable affects the independent variable. A good  alternative hypothesis example is “Attending physiotherapy sessions improves athletes' on-field performance.” or “Water evaporates at 100 °C. ” The alternative hypothesis further branches into directional and non-directional.

• Directional hypothesis: A hypothesis that states the result would be either positive or negative is called directional hypothesis. It accompanies H1 with either the ‘<' or ‘>' sign.
• Non-directional hypothesis: A non-directional hypothesis only claims an effect on the dependent variable. It does not clarify whether the result would be positive or negative. The sign for a non-directional hypothesis is ‘≠.'

3. Simple hypothesis

A simple hypothesis is a statement made to reflect the relation between exactly two variables. One independent and one dependent. Consider the example, “Smoking is a prominent cause of lung cancer." The dependent variable, lung cancer, is dependent on the independent variable, smoking.

4. Complex hypothesis

In contrast to a simple hypothesis, a complex hypothesis implies the relationship between multiple independent and dependent variables. For instance, “Individuals who eat more fruits tend to have higher immunity, lesser cholesterol, and high metabolism.” The independent variable is eating more fruits, while the dependent variables are higher immunity, lesser cholesterol, and high metabolism.

5. Associative and casual hypothesis

Associative and casual hypotheses don't exhibit how many variables there will be. They define the relationship between the variables. In an associative hypothesis, changing any one variable, dependent or independent, affects others. In a casual hypothesis, the independent variable directly affects the dependent.

6. Empirical hypothesis

Also referred to as the working hypothesis, an empirical hypothesis claims a theory's validation via experiments and observation. This way, the statement appears justifiable and different from a wild guess.

Say, the hypothesis is “Women who take iron tablets face a lesser risk of anemia than those who take vitamin B12.” This is an example of an empirical hypothesis where the researcher  the statement after assessing a group of women who take iron tablets and charting the findings.

7. Statistical hypothesis

The point of a statistical hypothesis is to test an already existing hypothesis by studying a population sample. Hypothesis like “44% of the Indian population belong in the age group of 22-27.” leverage evidence to prove or disprove a particular statement.

Characteristics of a Good Hypothesis

Writing a hypothesis is essential as it can make or break your research for you. That includes your chances of getting published in a journal. So when you're designing one, keep an eye out for these pointers:

• A research hypothesis has to be simple yet clear to look justifiable enough.
• It has to be testable — your research would be rendered pointless if too far-fetched into reality or limited by technology.
• It has to be precise about the results —what you are trying to do and achieve through it should come out in your hypothesis.
• A research hypothesis should be self-explanatory, leaving no doubt in the reader's mind.
• If you are developing a relational hypothesis, you need to include the variables and establish an appropriate relationship among them.
• A hypothesis must keep and reflect the scope for further investigations and experiments.

Separating a Hypothesis from a Prediction

Outside of academia, hypothesis and prediction are often used interchangeably. In research writing, this is not only confusing but also incorrect. And although a hypothesis and prediction are guesses at their core, there are many differences between them.

A hypothesis is an educated guess or even a testable prediction validated through research. It aims to analyze the gathered evidence and facts to define a relationship between variables and put forth a logical explanation behind the nature of events.

Predictions are assumptions or expected outcomes made without any backing evidence. They are more fictionally inclined regardless of where they originate from.

For this reason, a hypothesis holds much more weight than a prediction. It sticks to the scientific method rather than pure guesswork. "Planets revolve around the Sun." is an example of a hypothesis as it is previous knowledge and observed trends. Additionally, we can test it through the scientific method.

Whereas "COVID-19 will be eradicated by 2030." is a prediction. Even though it results from past trends, we can't prove or disprove it. So, the only way this gets validated is to wait and watch if COVID-19 cases end by 2030.

Finally, How to Write a Hypothesis

Quick tips on writing a hypothesis

1.  Be clear about your research question

A hypothesis should instantly address the research question or the problem statement. To do so, you need to ask a question. Understand the constraints of your undertaken research topic and then formulate a simple and topic-centric problem. Only after that can you develop a hypothesis and further test for evidence.

2. Carry out a recce

Once you have your research's foundation laid out, it would be best to conduct preliminary research. Go through previous theories, academic papers, data, and experiments before you start curating your research hypothesis. It will give you an idea of your hypothesis's viability or originality.

Making use of references from relevant research papers helps draft a good research hypothesis. SciSpace Discover offers a repository of over 270 million research papers to browse through and gain a deeper understanding of related studies on a particular topic. Additionally, you can use SciSpace Copilot , your AI research assistant, for reading any lengthy research paper and getting a more summarized context of it. A hypothesis can be formed after evaluating many such summarized research papers. Copilot also offers explanations for theories and equations, explains paper in simplified version, allows you to highlight any text in the paper or clip math equations and tables and provides a deeper, clear understanding of what is being said. This can improve the hypothesis by helping you identify potential research gaps.

3. Create a 3-dimensional hypothesis

Variables are an essential part of any reasonable hypothesis. So, identify your independent and dependent variable(s) and form a correlation between them. The ideal way to do this is to write the hypothetical assumption in the ‘if-then' form. If you use this form, make sure that you state the predefined relationship between the variables.

In another way, you can choose to present your hypothesis as a comparison between two variables. Here, you must specify the difference you expect to observe in the results.

4. Write the first draft

Now that everything is in place, it's time to write your hypothesis. For starters, create the first draft. In this version, write what you expect to find from your research.

Clearly separate your independent and dependent variables and the link between them. Don't fixate on syntax at this stage. The goal is to ensure your hypothesis addresses the issue.

5. Proof your hypothesis

After preparing the first draft of your hypothesis, you need to inspect it thoroughly. It should tick all the boxes, like being concise, straightforward, relevant, and accurate. Your final hypothesis has to be well-structured as well.

Research projects are an exciting and crucial part of being a scholar. And once you have your research question, you need a great hypothesis to begin conducting research. Thus, knowing how to write a hypothesis is very important.

Now that you have a firmer grasp on what a good hypothesis constitutes, the different kinds there are, and what process to follow, you will find it much easier to write your hypothesis, which ultimately helps your research.

Now it's easier than ever to streamline your research workflow with SciSpace Discover . Its integrated, comprehensive end-to-end platform for research allows scholars to easily discover, write and publish their research and fosters collaboration.

It includes everything you need, including a repository of over 270 million research papers across disciplines, SEO-optimized summaries and public profiles to show your expertise and experience.

If you found these tips on writing a research hypothesis useful, head over to our blog on Statistical Hypothesis Testing to learn about the top researchers, papers, and institutions in this domain.

Frequently Asked Questions (FAQs)

1. what is the definition of hypothesis.

According to the Oxford dictionary, a hypothesis is defined as “An idea or explanation of something that is based on a few known facts, but that has not yet been proved to be true or correct”.

2. What is an example of hypothesis?

The hypothesis is a statement that proposes a relationship between two or more variables. An example: "If we increase the number of new users who join our platform by 25%, then we will see an increase in revenue."

3. What is an example of null hypothesis?

A null hypothesis is a statement that there is no relationship between two variables. The null hypothesis is written as H0. The null hypothesis states that there is no effect. For example, if you're studying whether or not a particular type of exercise increases strength, your null hypothesis will be "there is no difference in strength between people who exercise and people who don't."

4. What are the types of research?

• Fundamental research

• Applied research

• Qualitative research

• Quantitative research

• Mixed research

• Exploratory research

• Longitudinal research

• Cross-sectional research

• Field research

• Laboratory research

• Fixed research

• Flexible research

• Action research

• Policy research

• Classification research

• Comparative research

• Causal research

• Inductive research

• Deductive research

5. How to write a hypothesis?

• Your hypothesis should be able to predict the relationship and outcome.

• Avoid wordiness by keeping it simple and brief.

• Your hypothesis should contain observable and testable outcomes.

• Your hypothesis should be relevant to the research question.

6. What are the 2 types of hypothesis?

• Null hypotheses are used to test the claim that "there is no difference between two groups of data".

• Alternative hypotheses test the claim that "there is a difference between two data groups".

7. Difference between research question and research hypothesis?

A research question is a broad, open-ended question you will try to answer through your research. A hypothesis is a statement based on prior research or theory that you expect to be true due to your study. Example - Research question: What are the factors that influence the adoption of the new technology? Research hypothesis: There is a positive relationship between age, education and income level with the adoption of the new technology.

8. What is plural for hypothesis?

The plural of hypothesis is hypotheses. Here's an example of how it would be used in a statement, "Numerous well-considered hypotheses are presented in this part, and they are supported by tables and figures that are well-illustrated."

9. What is the red queen hypothesis?

The red queen hypothesis in evolutionary biology states that species must constantly evolve to avoid extinction because if they don't, they will be outcompeted by other species that are evolving. Leigh Van Valen first proposed it in 1973; since then, it has been tested and substantiated many times.

10. Who is known as the father of null hypothesis?

The father of the null hypothesis is Sir Ronald Fisher. He published a paper in 1925 that introduced the concept of null hypothesis testing, and he was also the first to use the term itself.

11. When to reject null hypothesis?

You need to find a significant difference between your two populations to reject the null hypothesis. You can determine that by running statistical tests such as an independent sample t-test or a dependent sample t-test. You should reject the null hypothesis if the p-value is less than 0.05.

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How to Write a Hypothesis? Types and Examples 

All research studies involve the use of the scientific method, which is a mathematical and experimental technique used to conduct experiments by developing and testing a hypothesis or a prediction about an outcome. Simply put, a hypothesis is a suggested solution to a problem. It includes elements that are expressed in terms of relationships with each other to explain a condition or an assumption that hasn’t been verified using facts. 1 The typical steps in a scientific method include developing such a hypothesis, testing it through various methods, and then modifying it based on the outcomes of the experiments.  

A research hypothesis can be defined as a specific, testable prediction about the anticipated results of a study. 2 Hypotheses help guide the research process and supplement the aim of the study. After several rounds of testing, hypotheses can help develop scientific theories. 3 Hypotheses are often written as if-then statements. 

Here are two hypothesis examples: 

Dandelions growing in nitrogen-rich soils for two weeks develop larger leaves than those in nitrogen-poor soils because nitrogen stimulates vegetative growth. 4  

If a company offers flexible work hours, then their employees will be happier at work. 5  

Table of Contents

• What is a hypothesis? 
• Types of hypotheses 
• Characteristics of a hypothesis 
• Functions of a hypothesis 
• How to write a hypothesis 
• Hypothesis examples 
• Frequently asked questions 

What is a hypothesis?

A hypothesis expresses an expected relationship between variables in a study and is developed before conducting any research. Hypotheses are not opinions but rather are expected relationships based on facts and observations. They help support scientific research and expand existing knowledge. An incorrectly formulated hypothesis can affect the entire experiment leading to errors in the results so it’s important to know how to formulate a hypothesis and develop it carefully.

A few sources of a hypothesis include observations from prior studies, current research and experiences, competitors, scientific theories, and general conditions that can influence people. Figure 1 depicts the different steps in a research design and shows where exactly in the process a hypothesis is developed. 4  

There are seven different types of hypotheses—simple, complex, directional, nondirectional, associative and causal, null, and alternative. 

Types of hypotheses

The seven types of hypotheses are listed below: 5 , 6,7  

• Simple : Predicts the relationship between a single dependent variable and a single independent variable. 

Example: Exercising in the morning every day will increase your productivity.  

• Complex : Predicts the relationship between two or more variables. 

Example: Spending three hours or more on social media daily will negatively affect children’s mental health and productivity, more than that of adults.  

• Directional : Specifies the expected direction to be followed and uses terms like increase, decrease, positive, negative, more, or less. 

Example: The inclusion of intervention X decreases infant mortality compared to the original treatment.  

• Non-directional : Does not predict the exact direction, nature, or magnitude of the relationship between two variables but rather states the existence of a relationship. This hypothesis may be used when there is no underlying theory or if findings contradict prior research. 

Example: Cats and dogs differ in the amount of affection they express.  

• Associative and causal : An associative hypothesis suggests an interdependency between variables, that is, how a change in one variable changes the other.  

Example: There is a positive association between physical activity levels and overall health.  

A causal hypothesis, on the other hand, expresses a cause-and-effect association between variables. 

Example: Long-term alcohol use causes liver damage.  

• Null : Claims that the original hypothesis is false by showing that there is no relationship between the variables. 

Example: Sleep duration does not have any effect on productivity.  

• Alternative : States the opposite of the null hypothesis, that is, a relationship exists between two variables. 

Example: Sleep duration affects productivity.  

Characteristics of a hypothesis

So, what makes a good hypothesis? Here are some important characteristics of a hypothesis. 8,9  

• Testable : You must be able to test the hypothesis using scientific methods to either accept or reject the prediction. 
• Falsifiable : It should be possible to collect data that reject rather than support the hypothesis. 
• Logical : Hypotheses shouldn’t be a random guess but rather should be based on previous theories, observations, prior research, and logical reasoning. 
• Positive : The hypothesis statement about the existence of an association should be positive, that is, it should not suggest that an association does not exist. Therefore, the language used and knowing how to phrase a hypothesis is very important. 
• Clear and accurate : The language used should be easily comprehensible and use correct terminology. 
• Relevant : The hypothesis should be relevant and specific to the research question. 
• Structure : Should include all the elements that make a good hypothesis: variables, relationship, and outcome. 

Functions of a hypothesis

The following list mentions some important functions of a hypothesis: 1  

• Maintains the direction and progress of the research. 
• Expresses the important assumptions underlying the proposition in a single statement. 
• Establishes a suitable context for researchers to begin their investigation and for readers who are referring to the final report. 
• Provides an explanation for the occurrence of a specific phenomenon. 
• Ensures selection of appropriate and accurate facts necessary and relevant to the research subject. 

To summarize, a hypothesis provides the conceptual elements that complete the known data, conceptual relationships that systematize unordered elements, and conceptual meanings and interpretations that explain the unknown phenomena. 1  

How to write a hypothesis

Listed below are the main steps explaining how to write a hypothesis. 2,4,5  

• Make an observation and identify variables : Observe the subject in question and try to recognize a pattern or a relationship between the variables involved. This step provides essential background information to begin your research.  

For example, if you notice that an office’s vending machine frequently runs out of a specific snack, you may predict that more people in the office choose that snack over another. 

• Identify the main research question : After identifying a subject and recognizing a pattern, the next step is to ask a question that your hypothesis will answer.  

For example, after observing employees’ break times at work, you could ask “why do more employees take breaks in the morning rather than in the afternoon?” 

• Conduct some preliminary research to ensure originality and novelty : Your initial answer, which is your hypothesis, to the question is based on some pre-existing information about the subject. However, to ensure that your hypothesis has not been asked before or that it has been asked but rejected by other researchers you would need to gather additional information.  

For example, based on your observations you might state a hypothesis that employees work more efficiently when the air conditioning in the office is set at a lower temperature. However, during your preliminary research you find that this hypothesis was proven incorrect by a prior study. 

• Develop a general statement : After your preliminary research has confirmed the originality of your proposed answer, draft a general statement that includes all variables, subjects, and predicted outcome. The statement could be if/then or declarative.  
• Finalize the hypothesis statement : Use the PICOT model, which clarifies how to word a hypothesis effectively, when finalizing the statement. This model lists the important components required to write a hypothesis. 

P opulation: The specific group or individual who is the main subject of the research 

I nterest: The main concern of the study/research question 

C omparison: The main alternative group 

O utcome: The expected results  

T ime: Duration of the experiment 

Once you’ve finalized your hypothesis statement you would need to conduct experiments to test whether the hypothesis is true or false. 

Hypothesis examples

The following table provides examples of different types of hypotheses. 10 ,11  

Key takeaways  

Here’s a summary of all the key points discussed in this article about how to write a hypothesis. 

• A hypothesis is an assumption about an association between variables made based on limited evidence, which should be tested. 
• A hypothesis has four parts—the research question, independent variable, dependent variable, and the proposed relationship between the variables.   
• The statement should be clear, concise, testable, logical, and falsifiable. 
• There are seven types of hypotheses—simple, complex, directional, non-directional, associative and causal, null, and alternative. 
• A hypothesis provides a focus and direction for the research to progress. 
• A hypothesis plays an important role in the scientific method by helping to create an appropriate experimental design. 

Frequently asked questions

Hypotheses and research questions have different objectives and structure. The following table lists some major differences between the two. 9  

Here are a few examples to differentiate between a research question and hypothesis. 

Yes, here’s a simple checklist to help you gauge the effectiveness of your hypothesis. 9   1. When writing a hypothesis statement, check if it:  2. Predicts the relationship between the stated variables and the expected outcome.  3. Uses simple and concise language and is not wordy.  4. Does not assume readers’ knowledge about the subject.  5. Has observable, falsifiable, and testable results. 

As mentioned earlier in this article, a hypothesis is an assumption or prediction about an association between variables based on observations and simple evidence. These statements are usually generic. Research objectives, on the other hand, are more specific and dictated by hypotheses. The same hypothesis can be tested using different methods and the research objectives could be different in each case.     For example, Louis Pasteur observed that food lasts longer at higher altitudes, reasoned that it could be because the air at higher altitudes is cleaner (with fewer or no germs), and tested the hypothesis by exposing food to air cleaned in the laboratory. 12 Thus, a hypothesis is predictive—if the reasoning is correct, X will lead to Y—and research objectives are developed to test these predictions. 

Null hypothesis testing is a method to decide between two assumptions or predictions between variables (null and alternative hypotheses) in a statistical relationship in a sample. The null hypothesis, denoted as H 0 , claims that no relationship exists between variables in a population and any relationship in the sample reflects a sampling error or occurrence by chance. The alternative hypothesis, denoted as H 1 , claims that there is a relationship in the population. In every study, researchers need to decide whether the relationship in a sample occurred by chance or reflects a relationship in the population. This is done by hypothesis testing using the following steps: 13   1. Assume that the null hypothesis is true.  2. Determine how likely the sample relationship would be if the null hypothesis were true. This probability is called the p value.  3. If the sample relationship would be extremely unlikely, reject the null hypothesis and accept the alternative hypothesis. If the relationship would not be unlikely, accept the null hypothesis. 

To summarize, researchers should know how to write a good hypothesis to ensure that their research progresses in the required direction. A hypothesis is a testable prediction about any behavior or relationship between variables, usually based on facts and observation, and states an expected outcome.  

We hope this article has provided you with essential insight into the different types of hypotheses and their functions so that you can use them appropriately in your next research project. 

References  

• Dalen, DVV. The function of hypotheses in research. Proquest website. Accessed April 8, 2024. https://www.proquest.com/docview/1437933010?pq-origsite=gscholar&fromopenview=true&sourcetype=Scholarly%20Journals&imgSeq=1  
• McLeod S. Research hypothesis in psychology: Types & examples. SimplyPsychology website. Updated December 13, 2023. Accessed April 9, 2024. https://www.simplypsychology.org/what-is-a-hypotheses.html  
• Scientific method. Britannica website. Updated March 14, 2024. Accessed April 9, 2024. https://www.britannica.com/science/scientific-method  
• The hypothesis in science writing. Accessed April 10, 2024. https://berks.psu.edu/sites/berks/files/campus/HypothesisHandout_Final.pdf  
• How to develop a hypothesis (with elements, types, and examples). Indeed.com website. Updated February 3, 2023. Accessed April 10, 2024. https://www.indeed.com/career-advice/career-development/how-to-write-a-hypothesis  
• Types of research hypotheses. Excelsior online writing lab. Accessed April 11, 2024. https://owl.excelsior.edu/research/research-hypotheses/types-of-research-hypotheses/  
• What is a research hypothesis: how to write it, types, and examples. Researcher.life website. Published February 8, 2023. Accessed April 11, 2024. https://researcher.life/blog/article/how-to-write-a-research-hypothesis-definition-types-examples/  
• Developing a hypothesis. Pressbooks website. Accessed April 12, 2024. https://opentext.wsu.edu/carriecuttler/chapter/developing-a-hypothesis/  
• What is and how to write a good hypothesis in research. Elsevier author services website. Accessed April 12, 2024. https://scientific-publishing.webshop.elsevier.com/manuscript-preparation/what-how-write-good-hypothesis-research/  
• How to write a great hypothesis. Verywellmind website. Updated March 12, 2023. Accessed April 13, 2024. https://www.verywellmind.com/what-is-a-hypothesis-2795239  
• 15 Hypothesis examples. Helpfulprofessor.com Published September 8, 2023. Accessed March 14, 2024. https://helpfulprofessor.com/hypothesis-examples/ 
• Editage insights. What is the interconnectivity between research objectives and hypothesis? Published February 24, 2021. Accessed April 13, 2024. https://www.editage.com/insights/what-is-the-interconnectivity-between-research-objectives-and-hypothesis  
• Understanding null hypothesis testing. BCCampus open publishing. Accessed April 16, 2024. https://opentextbc.ca/researchmethods/chapter/understanding-null-hypothesis-testing/#:~:text=In%20null%20hypothesis%20testing%2C%20this,said%20to%20be%20statistically%20significant  

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What Is A Research (Scientific) Hypothesis? A plain-language explainer + examples

By:  Derek Jansen (MBA)  | Reviewed By: Dr Eunice Rautenbach | June 2020

If you’re new to the world of research, or it’s your first time writing a dissertation or thesis, you’re probably noticing that the words “research hypothesis” and “scientific hypothesis” are used quite a bit, and you’re wondering what they mean in a research context .

“Hypothesis” is one of those words that people use loosely, thinking they understand what it means. However, it has a very specific meaning within academic research. So, it’s important to understand the exact meaning before you start hypothesizing. 

Research Hypothesis 101

• What is a hypothesis ?
• What is a research hypothesis (scientific hypothesis)?
• Requirements for a research hypothesis
• Definition of a research hypothesis
• The null hypothesis

What is a hypothesis?

Let’s start with the general definition of a hypothesis (not a research hypothesis or scientific hypothesis), according to the Cambridge Dictionary:

Hypothesis: an idea or explanation for something that is based on known facts but has not yet been proved.

In other words, it’s a statement that provides an explanation for why or how something works, based on facts (or some reasonable assumptions), but that has not yet been specifically tested . For example, a hypothesis might look something like this:

Hypothesis: sleep impacts academic performance.

This statement predicts that academic performance will be influenced by the amount and/or quality of sleep a student engages in – sounds reasonable, right? It’s based on reasonable assumptions , underpinned by what we currently know about sleep and health (from the existing literature). So, loosely speaking, we could call it a hypothesis, at least by the dictionary definition.

But that’s not good enough…

Unfortunately, that’s not quite sophisticated enough to describe a research hypothesis (also sometimes called a scientific hypothesis), and it wouldn’t be acceptable in a dissertation, thesis or research paper . In the world of academic research, a statement needs a few more criteria to constitute a true research hypothesis .

What is a research hypothesis?

A research hypothesis (also called a scientific hypothesis) is a statement about the expected outcome of a study (for example, a dissertation or thesis). To constitute a quality hypothesis, the statement needs to have three attributes – specificity , clarity and testability .

Let’s take a look at these more closely.

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Hypothesis Essential #1: Specificity & Clarity

A good research hypothesis needs to be extremely clear and articulate about both what’ s being assessed (who or what variables are involved ) and the expected outcome (for example, a difference between groups, a relationship between variables, etc.).

Let’s stick with our sleepy students example and look at how this statement could be more specific and clear.

Hypothesis: Students who sleep at least 8 hours per night will, on average, achieve higher grades in standardised tests than students who sleep less than 8 hours a night.

As you can see, the statement is very specific as it identifies the variables involved (sleep hours and test grades), the parties involved (two groups of students), as well as the predicted relationship type (a positive relationship). There’s no ambiguity or uncertainty about who or what is involved in the statement, and the expected outcome is clear.

Contrast that to the original hypothesis we looked at – “Sleep impacts academic performance” – and you can see the difference. “Sleep” and “academic performance” are both comparatively vague , and there’s no indication of what the expected relationship direction is (more sleep or less sleep). As you can see, specificity and clarity are key.

Hypothesis Essential #2: Testability (Provability)

A statement must be testable to qualify as a research hypothesis. In other words, there needs to be a way to prove (or disprove) the statement. If it’s not testable, it’s not a hypothesis – simple as that.

For example, consider the hypothesis we mentioned earlier:

Hypothesis: Students who sleep at least 8 hours per night will, on average, achieve higher grades in standardised tests than students who sleep less than 8 hours a night.  

We could test this statement by undertaking a quantitative study involving two groups of students, one that gets 8 or more hours of sleep per night for a fixed period, and one that gets less. We could then compare the standardised test results for both groups to see if there’s a statistically significant difference. 

Again, if you compare this to the original hypothesis we looked at – “Sleep impacts academic performance” – you can see that it would be quite difficult to test that statement, primarily because it isn’t specific enough. How much sleep? By who? What type of academic performance?

So, remember the mantra – if you can’t test it, it’s not a hypothesis 🙂

Defining A Research Hypothesis

You’re still with us? Great! Let’s recap and pin down a clear definition of a hypothesis.

A research hypothesis (or scientific hypothesis) is a statement about an expected relationship between variables, or explanation of an occurrence, that is clear, specific and testable.

So, when you write up hypotheses for your dissertation or thesis, make sure that they meet all these criteria. If you do, you’ll not only have rock-solid hypotheses but you’ll also ensure a clear focus for your entire research project.

What about the null hypothesis?

You may have also heard the terms null hypothesis , alternative hypothesis, or H-zero thrown around. At a simple level, the null hypothesis is the counter-proposal to the original hypothesis.

For example, if the hypothesis predicts that there is a relationship between two variables (for example, sleep and academic performance), the null hypothesis would predict that there is no relationship between those variables.

At a more technical level, the null hypothesis proposes that no statistical significance exists in a set of given observations and that any differences are due to chance alone.

And there you have it – hypotheses in a nutshell. 

If you have any questions, be sure to leave a comment below and we’ll do our best to help you. If you need hands-on help developing and testing your hypotheses, consider our private coaching service , where we hold your hand through the research journey.

Psst... there’s more!

This post was based on one of our popular Research Bootcamps . If you're working on a research project, you'll definitely want to check this out ...

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16 Comments

Very useful information. I benefit more from getting more information in this regard.

Very great insight,educative and informative. Please give meet deep critics on many research data of public international Law like human rights, environment, natural resources, law of the sea etc

In a book I read a distinction is made between null, research, and alternative hypothesis. As far as I understand, alternative and research hypotheses are the same. Can you please elaborate? Best Afshin

This is a self explanatory, easy going site. I will recommend this to my friends and colleagues.

Very good definition. How can I cite your definition in my thesis? Thank you. Is nul hypothesis compulsory in a research?

It’s a counter-proposal to be proven as a rejection

Please what is the difference between alternate hypothesis and research hypothesis?

It is a very good explanation. However, it limits hypotheses to statistically tasteable ideas. What about for qualitative researches or other researches that involve quantitative data that don’t need statistical tests?

In qualitative research, one typically uses propositions, not hypotheses.

could you please elaborate it more

I’ve benefited greatly from these notes, thank you.

This is very helpful

well articulated ideas are presented here, thank you for being reliable sources of information

Excellent. Thanks for being clear and sound about the research methodology and hypothesis (quantitative research)

I have only a simple question regarding the null hypothesis. – Is the null hypothesis (Ho) known as the reversible hypothesis of the alternative hypothesis (H1? – How to test it in academic research?

this is very important note help me much more

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What Are Examples of a Hypothesis?

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A hypothesis is an explanation for a set of observations. Here are examples of a scientific hypothesis.

Although you could state a scientific hypothesis in various ways, most hypotheses are either "If, then" statements or forms of the null hypothesis . The null hypothesis is sometimes called the "no difference" hypothesis. The null hypothesis is good for experimentation because it's simple to disprove. If you disprove a null hypothesis, that is evidence for a relationship between the variables you are examining.

Examples of Null Hypotheses

• Hyperactivity is unrelated to eating sugar.
• All daisies have the same number of petals.
• The number of pets in a household is unrelated to the number of people living in it.
• A person's preference for a shirt is unrelated to its color.

Examples of If, Then Hypotheses

• If you get at least 6 hours of sleep, you will do better on tests than if you get less sleep.
• If you drop a ball, it will fall toward the ground.
• If you drink coffee before going to bed, then it will take longer to fall asleep.
• If you cover a wound with a bandage, then it will heal with less scarring.

Improving a Hypothesis to Make It Testable

You may wish to revise your first hypothesis in order to make it easier to design an experiment to test. For example, let's say you have a bad breakout the morning after eating a lot of greasy food. You may wonder if there is a correlation between eating greasy food and getting pimples. You propose the hypothesis:

Eating greasy food causes pimples.

Next, you need to design an experiment to test this hypothesis. Let's say you decide to eat greasy food every day for a week and record the effect on your face. Then, as a control, you'll avoid greasy food for the next week and see what happens. Now, this is not a good experiment because it does not take into account other factors such as hormone levels, stress, sun exposure, exercise, or any number of other variables that might conceivably affect your skin.

The problem is that you cannot assign cause to your effect . If you eat french fries for a week and suffer a breakout, can you definitely say it was the grease in the food that caused it? Maybe it was the salt. Maybe it was the potato. Maybe it was unrelated to diet. You can't prove your hypothesis. It's much easier to disprove a hypothesis.

So, let's restate the hypothesis to make it easier to evaluate the data:

Getting pimples is unaffected by eating greasy food.

So, if you eat fatty food every day for a week and suffer breakouts and then don't break out the week that you avoid greasy food, you can be pretty sure something is up. Can you disprove the hypothesis? Probably not, since it is so hard to assign cause and effect. However, you can make a strong case that there is some relationship between diet and acne.

If your skin stays clear for the entire test, you may decide to accept your hypothesis . Again, you didn't prove or disprove anything, which is fine

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Scientific Hypotheses: Writing, Promoting, and Predicting Implications

Armen yuri gasparyan.

1 Departments of Rheumatology and Research and Development, Dudley Group NHS Foundation Trust (Teaching Trust of the University of Birmingham, UK), Russells Hall Hospital, Dudley, West Midlands, UK.

Lilit Ayvazyan

2 Department of Medical Chemistry, Yerevan State Medical University, Yerevan, Armenia.

Ulzhan Mukanova

3 Department of Surgical Disciplines, South Kazakhstan Medical Academy, Shymkent, Kazakhstan.

Marlen Yessirkepov

4 Department of Biology and Biochemistry, South Kazakhstan Medical Academy, Shymkent, Kazakhstan.

George D. Kitas

5 Arthritis Research UK Epidemiology Unit, University of Manchester, Manchester, UK.

Scientific hypotheses are essential for progress in rapidly developing academic disciplines. Proposing new ideas and hypotheses require thorough analyses of evidence-based data and predictions of the implications. One of the main concerns relates to the ethical implications of the generated hypotheses. The authors may need to outline potential benefits and limitations of their suggestions and target widely visible publication outlets to ignite discussion by experts and start testing the hypotheses. Not many publication outlets are currently welcoming hypotheses and unconventional ideas that may open gates to criticism and conservative remarks. A few scholarly journals guide the authors on how to structure hypotheses. Reflecting on general and specific issues around the subject matter is often recommended for drafting a well-structured hypothesis article. An analysis of influential hypotheses, presented in this article, particularly Strachan's hygiene hypothesis with global implications in the field of immunology and allergy, points to the need for properly interpreting and testing new suggestions. Envisaging the ethical implications of the hypotheses should be considered both by authors and journal editors during the writing and publishing process.

INTRODUCTION

We live in times of digitization that radically changes scientific research, reporting, and publishing strategies. Researchers all over the world are overwhelmed with processing large volumes of information and searching through numerous online platforms, all of which make the whole process of scholarly analysis and synthesis complex and sophisticated.

Current research activities are diversifying to combine scientific observations with analysis of facts recorded by scholars from various professional backgrounds. 1 Citation analyses and networking on social media are also becoming essential for shaping research and publishing strategies globally. 2 Learning specifics of increasingly interdisciplinary research studies and acquiring information facilitation skills aid researchers in formulating innovative ideas and predicting developments in interrelated scientific fields.

Arguably, researchers are currently offered more opportunities than in the past for generating new ideas by performing their routine laboratory activities, observing individual cases and unusual developments, and critically analyzing published scientific facts. What they need at the start of their research is to formulate a scientific hypothesis that revisits conventional theories, real-world processes, and related evidence to propose new studies and test ideas in an ethical way. 3 Such a hypothesis can be of most benefit if published in an ethical journal with wide visibility and exposure to relevant online databases and promotion platforms.

Although hypotheses are crucially important for the scientific progress, only few highly skilled researchers formulate and eventually publish their innovative ideas per se . Understandably, in an increasingly competitive research environment, most authors would prefer to prioritize their ideas by discussing and conducting tests in their own laboratories or clinical departments, and publishing research reports afterwards. However, there are instances when simple observations and research studies in a single center are not capable of explaining and testing new groundbreaking ideas. Formulating hypothesis articles first and calling for multicenter and interdisciplinary research can be a solution in such instances, potentially launching influential scientific directions, if not academic disciplines.

The aim of this article is to overview the importance and implications of infrequently published scientific hypotheses that may open new avenues of thinking and research.

Despite the seemingly established views on innovative ideas and hypotheses as essential research tools, no structured definition exists to tag the term and systematically track related articles. In 1973, the Medical Subject Heading (MeSH) of the U.S. National Library of Medicine introduced “Research Design” as a structured keyword that referred to the importance of collecting data and properly testing hypotheses, and indirectly linked the term to ethics, methods and standards, among many other subheadings.

One of the experts in the field defines “hypothesis” as a well-argued analysis of available evidence to provide a realistic (scientific) explanation of existing facts, fill gaps in public understanding of sophisticated processes, and propose a new theory or a test. 4 A hypothesis can be proven wrong partially or entirely. However, even such an erroneous hypothesis may influence progress in science by initiating professional debates that help generate more realistic ideas. The main ethical requirement for hypothesis authors is to be honest about the limitations of their suggestions. 5

EXAMPLES OF INFLUENTIAL SCIENTIFIC HYPOTHESES

Daily routine in a research laboratory may lead to groundbreaking discoveries provided the daily accounts are comprehensively analyzed and reproduced by peers. The discovery of penicillin by Sir Alexander Fleming (1928) can be viewed as a prime example of such discoveries that introduced therapies to treat staphylococcal and streptococcal infections and modulate blood coagulation. 6 , 7 Penicillin got worldwide recognition due to the inventor's seminal works published by highly prestigious and widely visible British journals, effective ‘real-world’ antibiotic therapy of pneumonia and wounds during World War II, and euphoric media coverage. 8 In 1945, Fleming, Florey and Chain got a much deserved Nobel Prize in Physiology or Medicine for the discovery that led to the mass production of the wonder drug in the U.S. and ‘real-world practice’ that tested the use of penicillin. What remained globally unnoticed is that Zinaida Yermolyeva, the outstanding Soviet microbiologist, created the Soviet penicillin, which turned out to be more effective than the Anglo-American penicillin and entered mass production in 1943; that year marked the turning of the tide of the Great Patriotic War. 9 One of the reasons of the widely unnoticed discovery of Zinaida Yermolyeva is that her works were published exclusively by local Russian (Soviet) journals.

The past decades have been marked by an unprecedented growth of multicenter and global research studies involving hundreds and thousands of human subjects. This trend is shaped by an increasing number of reports on clinical trials and large cohort studies that create a strong evidence base for practice recommendations. Mega-studies may help generate and test large-scale hypotheses aiming to solve health issues globally. Properly designed epidemiological studies, for example, may introduce clarity to the hygiene hypothesis that was originally proposed by David Strachan in 1989. 10 David Strachan studied the epidemiology of hay fever in a cohort of 17,414 British children and concluded that declining family size and improved personal hygiene had reduced the chances of cross infections in families, resulting in epidemics of atopic disease in post-industrial Britain. Over the past four decades, several related hypotheses have been proposed to expand the potential role of symbiotic microorganisms and parasites in the development of human physiological immune responses early in life and protection from allergic and autoimmune diseases later on. 11 , 12 Given the popularity and the scientific importance of the hygiene hypothesis, it was introduced as a MeSH term in 2012. 13

Hypotheses can be proposed based on an analysis of recorded historic events that resulted in mass migrations and spreading of certain genetic diseases. As a prime example, familial Mediterranean fever (FMF), the prototype periodic fever syndrome, is believed to spread from Mesopotamia to the Mediterranean region and all over Europe due to migrations and religious prosecutions millennia ago. 14 Genetic mutations spearing mild clinical forms of FMF are hypothesized to emerge and persist in the Mediterranean region as protective factors against more serious infectious diseases, particularly tuberculosis, historically common in that part of the world. 15 The speculations over the advantages of carrying the MEditerranean FeVer (MEFV) gene are further strengthened by recorded low mortality rates from tuberculosis among FMF patients of different nationalities living in Tunisia in the first half of the 20th century. 16

Diagnostic hypotheses shedding light on peculiarities of diseases throughout the history of mankind can be formulated using artefacts, particularly historic paintings. 17 Such paintings may reveal joint deformities and disfigurements due to rheumatic diseases in individual subjects. A series of paintings with similar signs of pathological conditions interpreted in a historic context may uncover mysteries of epidemics of certain diseases, which is the case with Ruben's paintings depicting signs of rheumatic hands and making some doctors to believe that rheumatoid arthritis was common in Europe in the 16th and 17th century. 18

WRITING SCIENTIFIC HYPOTHESES

There are author instructions of a few journals that specifically guide how to structure, format, and make submissions categorized as hypotheses attractive. One of the examples is presented by Med Hypotheses , the flagship journal in its field with more than four decades of publishing and influencing hypothesis authors globally. However, such guidance is not based on widely discussed, implemented, and approved reporting standards, which are becoming mandatory for all scholarly journals.

Generating new ideas and scientific hypotheses is a sophisticated task since not all researchers and authors are skilled to plan, conduct, and interpret various research studies. Some experience with formulating focused research questions and strong working hypotheses of original research studies is definitely helpful for advancing critical appraisal skills. However, aspiring authors of scientific hypotheses may need something different, which is more related to discerning scientific facts, pooling homogenous data from primary research works, and synthesizing new information in a systematic way by analyzing similar sets of articles. To some extent, this activity is reminiscent of writing narrative and systematic reviews. As in the case of reviews, scientific hypotheses need to be formulated on the basis of comprehensive search strategies to retrieve all available studies on the topics of interest and then synthesize new information selectively referring to the most relevant items. One of the main differences between scientific hypothesis and review articles relates to the volume of supportive literature sources ( Table 1 ). In fact, hypothesis is usually formulated by referring to a few scientific facts or compelling evidence derived from a handful of literature sources. 19 By contrast, reviews require analyses of a large number of published documents retrieved from several well-organized and evidence-based databases in accordance with predefined search strategies. 20 , 21 , 22

The format of hypotheses, especially the implications part, may vary widely across disciplines. Clinicians may limit their suggestions to the clinical manifestations of diseases, outcomes, and management strategies. Basic and laboratory scientists analysing genetic, molecular, and biochemical mechanisms may need to view beyond the frames of their narrow fields and predict social and population-based implications of the proposed ideas. 23

Advanced writing skills are essential for presenting an interesting theoretical article which appeals to the global readership. Merely listing opposing facts and ideas, without proper interpretation and analysis, may distract the experienced readers. The essence of a great hypothesis is a story behind the scientific facts and evidence-based data.

ETHICAL IMPLICATIONS

The authors of hypotheses substantiate their arguments by referring to and discerning rational points from published articles that might be overlooked by others. Their arguments may contradict the established theories and practices, and pose global ethical issues, particularly when more or less efficient medical technologies and public health interventions are devalued. The ethical issues may arise primarily because of the careless references to articles with low priorities, inadequate and apparently unethical methodologies, and concealed reporting of negative results. 24 , 25

Misinterpretation and misunderstanding of the published ideas and scientific hypotheses may complicate the issue further. For example, Alexander Fleming, whose innovative ideas of penicillin use to kill susceptible bacteria saved millions of lives, warned of the consequences of uncontrolled prescription of the drug. The issue of antibiotic resistance had emerged within the first ten years of penicillin use on a global scale due to the overprescription that affected the efficacy of antibiotic therapies, with undesirable consequences for millions. 26

The misunderstanding of the hygiene hypothesis that primarily aimed to shed light on the role of the microbiome in allergic and autoimmune diseases resulted in decline of public confidence in hygiene with dire societal implications, forcing some experts to abandon the original idea. 27 , 28 Although that hypothesis is unrelated to the issue of vaccinations, the public misunderstanding has resulted in decline of vaccinations at a time of upsurge of old and new infections.

A number of ethical issues are posed by the denial of the viral (human immunodeficiency viruses; HIV) hypothesis of acquired Immune deficiency Syndrome (AIDS) by Peter Duesberg, who overviewed the links between illicit recreational drugs and antiretroviral therapies with AIDS and refuted the etiological role of HIV. 29 That controversial hypothesis was rejected by several journals, but was eventually published without external peer review at Med Hypotheses in 2010. The publication itself raised concerns of the unconventional editorial policy of the journal, causing major perturbations and more scrutinized publishing policies by journals processing hypotheses.

WHERE TO PUBLISH HYPOTHESES

Although scientific authors are currently well informed and equipped with search tools to draft evidence-based hypotheses, there are still limited quality publication outlets calling for related articles. The journal editors may be hesitant to publish articles that do not adhere to any research reporting guidelines and open gates for harsh criticism of unconventional and untested ideas. Occasionally, the editors opting for open-access publishing and upgrading their ethics regulations launch a section to selectively publish scientific hypotheses attractive to the experienced readers. 30 However, the absence of approved standards for this article type, particularly no mandate for outlining potential ethical implications, may lead to publication of potentially harmful ideas in an attractive format.

A suggestion of simultaneously publishing multiple or alternative hypotheses to balance the reader views and feedback is a potential solution for the mainstream scholarly journals. 31 However, that option alone is hardly applicable to emerging journals with unconventional quality checks and peer review, accumulating papers with multiple rejections by established journals.

A large group of experts view hypotheses with improbable and controversial ideas publishable after formal editorial (in-house) checks to preserve the authors' genuine ideas and avoid conservative amendments imposed by external peer reviewers. 32 That approach may be acceptable for established publishers with large teams of experienced editors. However, the same approach can lead to dire consequences if employed by nonselective start-up, open-access journals processing all types of articles and primarily accepting those with charged publication fees. 33 In fact, pseudoscientific ideas arguing Newton's and Einstein's seminal works or those denying climate change that are hardly testable have already found their niche in substandard electronic journals with soft or nonexistent peer review. 34

CITATIONS AND SOCIAL MEDIA ATTENTION

The available preliminary evidence points to the attractiveness of hypothesis articles for readers, particularly those from research-intensive countries who actively download related documents. 35 However, citations of such articles are disproportionately low. Only a small proportion of top-downloaded hypotheses (13%) in the highly prestigious Med Hypotheses receive on average 5 citations per article within a two-year window. 36

With the exception of a few historic papers, the vast majority of hypotheses attract relatively small number of citations in a long term. 36 Plausible explanations are that these articles often contain a single or only a few citable points and that suggested research studies to test hypotheses are rarely conducted and reported, limiting chances of citing and crediting authors of genuine research ideas.

A snapshot analysis of citation activity of hypothesis articles may reveal interest of the global scientific community towards their implications across various disciplines and countries. As a prime example, Strachan's hygiene hypothesis, published in 1989, 10 is still attracting numerous citations on Scopus, the largest bibliographic database. As of August 28, 2019, the number of the linked citations in the database is 3,201. Of the citing articles, 160 are cited at least 160 times ( h -index of this research topic = 160). The first three citations are recorded in 1992 and followed by a rapid annual increase in citation activity and a peak of 212 in 2015 ( Fig. 1 ). The top 5 sources of the citations are Clin Exp Allergy (n = 136), J Allergy Clin Immunol (n = 119), Allergy (n = 81), Pediatr Allergy Immunol (n = 69), and PLOS One (n = 44). The top 5 citing authors are leading experts in pediatrics and allergology Erika von Mutius (Munich, Germany, number of publications with the index citation = 30), Erika Isolauri (Turku, Finland, n = 27), Patrick G Holt (Subiaco, Australia, n = 25), David P. Strachan (London, UK, n = 23), and Bengt Björksten (Stockholm, Sweden, n = 22). The U.S. is the leading country in terms of citation activity with 809 related documents, followed by the UK (n = 494), Germany (n = 314), Australia (n = 211), and the Netherlands (n = 177). The largest proportion of citing documents are articles (n = 1,726, 54%), followed by reviews (n = 950, 29.7%), and book chapters (n = 213, 6.7%). The main subject areas of the citing items are medicine (n = 2,581, 51.7%), immunology and microbiology (n = 1,179, 23.6%), and biochemistry, genetics and molecular biology (n = 415, 8.3%).

Interestingly, a recent analysis of 111 publications related to Strachan's hygiene hypothesis, stating that the lack of exposure to infections in early life increases the risk of rhinitis, revealed a selection bias of 5,551 citations on Web of Science. 37 The articles supportive of the hypothesis were cited more than nonsupportive ones (odds ratio adjusted for study design, 2.2; 95% confidence interval, 1.6–3.1). A similar conclusion pointing to a citation bias distorting bibliometrics of hypotheses was reached by an earlier analysis of a citation network linked to the idea that β-amyloid, which is involved in the pathogenesis of Alzheimer disease, is produced by skeletal muscle of patients with inclusion body myositis. 38 The results of both studies are in line with the notion that ‘positive’ citations are more frequent in the field of biomedicine than ‘negative’ ones, and that citations to articles with proven hypotheses are too common. 39

Social media channels are playing an increasingly active role in the generation and evaluation of scientific hypotheses. In fact, publicly discussing research questions on platforms of news outlets, such as Reddit, may shape hypotheses on health-related issues of global importance, such as obesity. 40 Analyzing Twitter comments, researchers may reveal both potentially valuable ideas and unfounded claims that surround groundbreaking research ideas. 41 Social media activities, however, are unevenly distributed across different research topics, journals and countries, and these are not always objective professional reflections of the breakthroughs in science. 2 , 42

Scientific hypotheses are essential for progress in science and advances in healthcare. Innovative ideas should be based on a critical overview of related scientific facts and evidence-based data, often overlooked by others. To generate realistic hypothetical theories, the authors should comprehensively analyze the literature and suggest relevant and ethically sound design for future studies. They should also consider their hypotheses in the context of research and publication ethics norms acceptable for their target journals. The journal editors aiming to diversify their portfolio by maintaining and introducing hypotheses section are in a position to upgrade guidelines for related articles by pointing to general and specific analyses of the subject, preferred study designs to test hypotheses, and ethical implications. The latter is closely related to specifics of hypotheses. For example, editorial recommendations to outline benefits and risks of a new laboratory test or therapy may result in a more balanced article and minimize associated risks afterwards.

Not all scientific hypotheses have immediate positive effects. Some, if not most, are never tested in properly designed research studies and never cited in credible and indexed publication outlets. Hypotheses in specialized scientific fields, particularly those hardly understandable for nonexperts, lose their attractiveness for increasingly interdisciplinary audience. The authors' honest analysis of the benefits and limitations of their hypotheses and concerted efforts of all stakeholders in science communication to initiate public discussion on widely visible platforms and social media may reveal rational points and caveats of the new ideas.

Disclosure: The authors have no potential conflicts of interest to disclose.

Author Contributions:

• Conceptualization: Gasparyan AY, Yessirkepov M, Kitas GD.
• Methodology: Gasparyan AY, Mukanova U, Ayvazyan L.
• Writing - original draft: Gasparyan AY, Ayvazyan L, Yessirkepov M.
• Writing - review & editing: Gasparyan AY, Yessirkepov M, Mukanova U, Kitas GD.

Research Hypothesis In Psychology: Types, & Examples

Saul Mcleod, PhD

Editor-in-Chief for Simply Psychology

BSc (Hons) Psychology, MRes, PhD, University of Manchester

Saul Mcleod, PhD., is a qualified psychology teacher with over 18 years of experience in further and higher education. He has been published in peer-reviewed journals, including the Journal of Clinical Psychology.

Learn about our Editorial Process

Olivia Guy-Evans, MSc

Associate Editor for Simply Psychology

BSc (Hons) Psychology, MSc Psychology of Education

Olivia Guy-Evans is a writer and associate editor for Simply Psychology. She has previously worked in healthcare and educational sectors.

On This Page:

A research hypothesis, in its plural form “hypotheses,” is a specific, testable prediction about the anticipated results of a study, established at its outset. It is a key component of the scientific method .

Hypotheses connect theory to data and guide the research process towards expanding scientific understanding

Some key points about hypotheses:

• A hypothesis expresses an expected pattern or relationship. It connects the variables under investigation.
• It is stated in clear, precise terms before any data collection or analysis occurs. This makes the hypothesis testable.
• A hypothesis must be falsifiable. It should be possible, even if unlikely in practice, to collect data that disconfirms rather than supports the hypothesis.
• Hypotheses guide research. Scientists design studies to explicitly evaluate hypotheses about how nature works.
• For a hypothesis to be valid, it must be testable against empirical evidence. The evidence can then confirm or disprove the testable predictions.
• Hypotheses are informed by background knowledge and observation, but go beyond what is already known to propose an explanation of how or why something occurs.

Predictions typically arise from a thorough knowledge of the research literature, curiosity about real-world problems or implications, and integrating this to advance theory. They build on existing literature while providing new insight.

Types of Research Hypotheses

Alternative hypothesis.

The research hypothesis is often called the alternative or experimental hypothesis in experimental research.

It typically suggests a potential relationship between two key variables: the independent variable, which the researcher manipulates, and the dependent variable, which is measured based on those changes.

The alternative hypothesis states a relationship exists between the two variables being studied (one variable affects the other).

A hypothesis is a testable statement or prediction about the relationship between two or more variables. It is a key component of the scientific method. Some key points about hypotheses:

• Important hypotheses lead to predictions that can be tested empirically. The evidence can then confirm or disprove the testable predictions.

In summary, a hypothesis is a precise, testable statement of what researchers expect to happen in a study and why. Hypotheses connect theory to data and guide the research process towards expanding scientific understanding.

An experimental hypothesis predicts what change(s) will occur in the dependent variable when the independent variable is manipulated.

It states that the results are not due to chance and are significant in supporting the theory being investigated.

The alternative hypothesis can be directional, indicating a specific direction of the effect, or non-directional, suggesting a difference without specifying its nature. It’s what researchers aim to support or demonstrate through their study.

Null Hypothesis

The null hypothesis states no relationship exists between the two variables being studied (one variable does not affect the other). There will be no changes in the dependent variable due to manipulating the independent variable.

It states results are due to chance and are not significant in supporting the idea being investigated.

The null hypothesis, positing no effect or relationship, is a foundational contrast to the research hypothesis in scientific inquiry. It establishes a baseline for statistical testing, promoting objectivity by initiating research from a neutral stance.

Many statistical methods are tailored to test the null hypothesis, determining the likelihood of observed results if no true effect exists.

This dual-hypothesis approach provides clarity, ensuring that research intentions are explicit, and fosters consistency across scientific studies, enhancing the standardization and interpretability of research outcomes.

Nondirectional Hypothesis

A non-directional hypothesis, also known as a two-tailed hypothesis, predicts that there is a difference or relationship between two variables but does not specify the direction of this relationship.

It merely indicates that a change or effect will occur without predicting which group will have higher or lower values.

For example, “There is a difference in performance between Group A and Group B” is a non-directional hypothesis.

Directional Hypothesis

A directional (one-tailed) hypothesis predicts the nature of the effect of the independent variable on the dependent variable. It predicts in which direction the change will take place. (i.e., greater, smaller, less, more)

It specifies whether one variable is greater, lesser, or different from another, rather than just indicating that there’s a difference without specifying its nature.

For example, “Exercise increases weight loss” is a directional hypothesis.

Falsifiability

The Falsification Principle, proposed by Karl Popper , is a way of demarcating science from non-science. It suggests that for a theory or hypothesis to be considered scientific, it must be testable and irrefutable.

Falsifiability emphasizes that scientific claims shouldn’t just be confirmable but should also have the potential to be proven wrong.

It means that there should exist some potential evidence or experiment that could prove the proposition false.

However many confirming instances exist for a theory, it only takes one counter observation to falsify it. For example, the hypothesis that “all swans are white,” can be falsified by observing a black swan.

For Popper, science should attempt to disprove a theory rather than attempt to continually provide evidence to support a research hypothesis.

Can a Hypothesis be Proven?

Hypotheses make probabilistic predictions. They state the expected outcome if a particular relationship exists. However, a study result supporting a hypothesis does not definitively prove it is true.

All studies have limitations. There may be unknown confounding factors or issues that limit the certainty of conclusions. Additional studies may yield different results.

In science, hypotheses can realistically only be supported with some degree of confidence, not proven. The process of science is to incrementally accumulate evidence for and against hypothesized relationships in an ongoing pursuit of better models and explanations that best fit the empirical data. But hypotheses remain open to revision and rejection if that is where the evidence leads.

• Disproving a hypothesis is definitive. Solid disconfirmatory evidence will falsify a hypothesis and require altering or discarding it based on the evidence.
• However, confirming evidence is always open to revision. Other explanations may account for the same results, and additional or contradictory evidence may emerge over time.

We can never 100% prove the alternative hypothesis. Instead, we see if we can disprove, or reject the null hypothesis.

If we reject the null hypothesis, this doesn’t mean that our alternative hypothesis is correct but does support the alternative/experimental hypothesis.

Upon analysis of the results, an alternative hypothesis can be rejected or supported, but it can never be proven to be correct. We must avoid any reference to results proving a theory as this implies 100% certainty, and there is always a chance that evidence may exist which could refute a theory.

How to Write a Hypothesis

• Identify variables . The researcher manipulates the independent variable and the dependent variable is the measured outcome.
• Operationalized the variables being investigated . Operationalization of a hypothesis refers to the process of making the variables physically measurable or testable, e.g. if you are about to study aggression, you might count the number of punches given by participants.
• Decide on a direction for your prediction . If there is evidence in the literature to support a specific effect of the independent variable on the dependent variable, write a directional (one-tailed) hypothesis. If there are limited or ambiguous findings in the literature regarding the effect of the independent variable on the dependent variable, write a non-directional (two-tailed) hypothesis.
• Make it Testable : Ensure your hypothesis can be tested through experimentation or observation. It should be possible to prove it false (principle of falsifiability).
• Clear & concise language . A strong hypothesis is concise (typically one to two sentences long), and formulated using clear and straightforward language, ensuring it’s easily understood and testable.

Consider a hypothesis many teachers might subscribe to: students work better on Monday morning than on Friday afternoon (IV=Day, DV= Standard of work).

Now, if we decide to study this by giving the same group of students a lesson on a Monday morning and a Friday afternoon and then measuring their immediate recall of the material covered in each session, we would end up with the following:

• The alternative hypothesis states that students will recall significantly more information on a Monday morning than on a Friday afternoon.
• The null hypothesis states that there will be no significant difference in the amount recalled on a Monday morning compared to a Friday afternoon. Any difference will be due to chance or confounding factors.

More Examples

• Memory : Participants exposed to classical music during study sessions will recall more items from a list than those who studied in silence.
• Social Psychology : Individuals who frequently engage in social media use will report higher levels of perceived social isolation compared to those who use it infrequently.
• Developmental Psychology : Children who engage in regular imaginative play have better problem-solving skills than those who don’t.
• Clinical Psychology : Cognitive-behavioral therapy will be more effective in reducing symptoms of anxiety over a 6-month period compared to traditional talk therapy.
• Cognitive Psychology : Individuals who multitask between various electronic devices will have shorter attention spans on focused tasks than those who single-task.
• Health Psychology : Patients who practice mindfulness meditation will experience lower levels of chronic pain compared to those who don’t meditate.
• Organizational Psychology : Employees in open-plan offices will report higher levels of stress than those in private offices.
• Behavioral Psychology : Rats rewarded with food after pressing a lever will press it more frequently than rats who receive no reward.

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• Published: 10 August 2016

Research protocols: A forest of hypotheses

• Julia Rosen 1  

Nature volume  536 ,  pages 239–241 ( 2016 ) Cite this article

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• Research management

Falling in love with a single theory can cut off fruitful avenues of enquiry. Here's how to keep your mind open.

The clamour in a Panamanian rainforest is deafening to human ears: bugs shriek, birds sing and bats screech throughout the humid night. To avoid attracting predators, male katydids ( Tettigoniidae ) trill out short, infrequent mating calls less than a second long.

Postdoc Laurel Symes, who studies sensory perception and decision-making at Dartmouth College in Hanover, New Hampshire, wants to understand how female katydids find their mates. She first thought they must have highly sensitive hearing. But she juggles other ideas at the same time: maybe katydids always meet up on a certain type of host plant, have neural mechanisms that filter out background noise or use another trick entirely.

These aren't just idle musings: Symes's collection of hypotheses is an integral part of her research. The approach helps her to home in on answers and avoid investment in a sole idea — a common tendency in science that can lead to trouble. History contains numerous examples of scientists who missed important clues because they clung too tightly to a favourite hypothesis. One way to avoid this fate is to consider many potential hypotheses.

Proponents of the multiple-working-hypotheses method say that it prevents scientists from developing 'tunnel vision', and enables them to embrace the possibility that several hypotheses might be true at once. Practising the approach takes discipline: researchers must brainstorm possible explanations for a scientific phenomenon before collecting or analysing data, and use techniques such as scrambling the order of samples and blinding data to help to counteract favouritism. It also demands that scientists remain open-minded during the entire research process, and continually refine their hypotheses.

A long history

The method of multiple working hypotheses was formally articulated 1 in 1890 by geologist Thomas Chrowder Chamberlin, then president of the University of Wisconsin–Madison. Building on the ideas of fellow geologist Grove Karl Gilbert, Chamberlin warned that when scientists come up with an original idea, they tend to develop affection for it, which can cloud their ability to do objective work. He argued that the solution was to generate and explore a family of hypotheses. By coming up with alternatives, he suggested, scientists would not be inclined to favour one idea.

If the hypotheses are mutually exclusive, you are going to learn something.

Although the concept has faced criticism, aimed mainly at the impossibility of conceiving — let alone testing — all possibilities, many scientists say that it is as relevant today as ever. The pressure to publish in high-profile journals, win grants and build a reputation can prompt researchers — consciously or not — to seek support for pet ideas. One study posted to the preprint server arXiv 2 in June found that when programmers introduced these kinds of incentives into a model, simulated research groups succumbed to pressures to show support for original ideas, often erroneously.

Ecologist Barry Brook of the University of Tasmania in Australia thinks that resurrecting Chamberlin's ideas could help. In 2007, he co-authored a paper on the merits of using multiple working hypotheses for twenty-first-century science 3 . In many cases, he argues, the method produces more insightful results than testing null hypotheses, which reveals only whether a specific factor has a discernible effect. Multiple hypotheses, by contrast, can help scientists to work out whether that effect is important, and whether several factors might be at play.

Brook, for example, wanted to know why small mammals such as brown bandicoots ( Isoodon macrourus ) were disappearing from Northern Australia's Kakadu National Park. Many scientists had pointed in the past to introduced predators, such as cats, which seemed plausible. But when he considered other hypotheses and looked at historical population data, he found that cats had a negligible role, and that intense wildfires bore most blame 4 . “You can be surprised at how little support most of your well-crafted hypotheses can have,” he says.

It might seem simpler to consider just one possible explanation, but ignoring other models can be dangerous. “That's not only dishonest, but it will also lead you down bad inferential pathways,” Brook says.

Resist temptation

It can be challenging to put the method into practice because researchers must battle their own natural enthusiasm for an alluring idea. The first step is to set aside time to articulate other hypotheses before one starts to gain traction. If not, a favoured hypothesis might skew the process of data collection or analysis when one heads out into the field, starts an experiment or dives into a data set. “If you have a hypothesis or you're looking for a pattern, sometimes you won't actually honour what pattern is there,” says Kathleen Nicoll, a geographer at the University of Utah in Salt Lake City.

When coming up with a collection of hypotheses, it can be helpful to have patience and consult labmates — and to include a seemingly outrageous hypothesis. This idea was first advocated in 1926 by William Morris Davis, a retired geologist from Harvard University in Cambridge, Massachusetts, as a way to break out of conventional thinking. Many notable scientific advances fall into this category, including Alfred Wegener's then-scandalous claim in 1912 that continents migrate across Earth's surface 5 (they do), and the heretical proposal, developed in the 1920s by geologist J Harlen Bretz, that a catastrophic flood scoured out the heavily channeled landscapes of Washington (in fact, many violent floods swept through the region).

Symes finds that using multiple hypotheses yields the best results if researchers generate ideas that rely on different processes and make distinct predictions. In her research, a host-plant preference might lead to katydids having the same food in their guts, whereas using sound might imply that female katydids in Panama have more sensitive ears than species in forests without predatory bats. By identifying possible outcomes, she can design her experiments in ways that help to distinguish these ideas. “If the hypotheses are mutually exclusive or different in their mechanism, then you are going to learn something,” she says.

Consideration of multiple working hypotheses continues during data processing and analysis, when scientists must take other steps to protect their objectivity (see 'Don't play favourites' ).

For Lydia Tackett, who studies marine fossils at North Dakota State University in Fargo, the solution is as simple as analysing samples out of order. Working chronologically through a geological sequence led her to identify trends prematurely and anticipate what she would find in subsequent layers. “Now, I collect the bulk samples I need and randomize the order,” she says. She codes them so that she doesn't know exactly which layer each sample came from.

Others rely on statistical tools. Instead of using P values to reject individual models one at a time, Trevor Branch, a fisheries scientist at the University of Washington in Seattle, embraces a model-selection technique called Akaike's information criterion (AIC). This statistical method determines which of a set of models best explains data collected about an often-complex system. Branch says that it's a mathematical way of implementing Chamberlin's method of multiple working hypotheses.

Brook uses the AIC as well as the similar Bayesian information criterion, which is useful for distinguishing between a few simple models. When several models seem to be true, these methods help to weight their relative importance, so that their combined effects can be explored through something called multimodel inference. That involves merging several different models and considering them simultaneously to explain as much as possible.

Physicists and astronomers often take extreme measures to prevent researcher bias from creeping into their analyses. Saul Perlmutter, an astrophysicist at the University of California, Berkeley, relies on software or colleagues to hide potentially telling clues in the data before he sees them, a technique called blind analysis. This might include adding randomly generated numbers to data values, shifting them by random amounts or hiding the axes on a graph. The goal is to make sure that the researchers don't see anything that could prime their minds towards a particular interpretation, such as a preliminary trend or hint of a discovery.

Before unblinding data, scientists on Perlmutter's team must circulate a memo explaining their hypotheses and how they plan to test and differentiate between them. “Everybody can decide ahead of time whether that feels fair — that they haven't treated any of the alternatives differently than the others,” he says. Last year, Perlmutter and psychologist Robert MacCoun of Stanford University in California argued in a Nature Comment 6 that this approach could reduce researcher bias in many fields.

Of course, there are situations in which multiple hypotheses aren't helpful — or even feasible. If researchers stumble on a mysterious finding, they might struggle to come up with even a single plausible explanation. And even if they can cobble together a few, there is no guarantee that the correct hypothesis is among them. This is why hypotheses must remain 'working', so that they can be refined in light of new information.

Other situations present the opposite challenge: too many hypotheses. Freya Blekman, an experimental physicist at the Dutch-speaking Free University of Brussels, searches for elementary particles at facilities such as the Large Hadron Collider at CERN, Europe's particle-physics lab near Geneva, Switerland. In her field, theorists have already posited countless possibilities, and her task is to work out which ones the evidence supports.

Because these models are often mutually exclusive, she typically evaluates them one at a time using P values — albeit held to an exceptionally high standard of significance. In fields such as psychology and medicine, there is a growing movement to abandon this technique because it can tempt researchers to seek out analytical approaches that produce significant results. But Blekman says that the physics community has largely eliminated this problem through blinding and by creating a culture so steeped in the ethos of multiple hypotheses that finding nothing is as important as finding something. “In our field, a null result is a valuable result,” she says.

Indeed, the method of multiple hypotheses doesn't always have to be practised at the individual level, and can take place across entire fields. Different groups can advance various hypotheses, as long as they remain open-minded, and the peer-review process can also help to promote the practice. “I think we have a duty as editors and reviewers to bring up alternatives,” says Branch, “and to require authors that come up with a new hypothesis to also include alternatives when they bring it up the first time around”.

Regardless of how they apply the method, many researchers say that they stumbled across the idea of multiple hypotheses by accident, as graduate students or later. Branch had never heard of the concept until a few years ago, but was so struck by it that he wrote an article last year arguing that researchers should not seek a single, universal explanation for how fisheries affect marine food webs, but should consider how different models might apply in various parts of the world 7 .

A few researchers say that their advisers encouraged them to read classic philosophy-of-science texts, such as Thomas Kuhn's Structure of Scientific Revolutions (Univ. Chicago Press, 1962), or fostered discussions on the practical side of the scientific method at lab meetings. But many scientists can make it through their entire careers without any formal training in how to develop hypotheses.

That's too bad, because learning and applying the multiple-hypothesis method can improve the calibre of scientists' work and empower scientists themselves, says Symes, who published a guide last year on teaching the research process 8 . “It always pains me to see students who define success and failure as whether they support a particular hypothesis,” she says. “Failing is not collecting the data you need. Succeeding is being able to differentiate the possibilities.”

Box 1: Don't play favourites

To apply the multiple-working-hypotheses method, try these tips:

Devise a list of possible hypotheses before collecting or looking at new data.

Talk to colleagues and try to challenge your assumptions by creating at least one outrageous hypothesis.

To learn most efficiently, develop hypotheses that are as distinct from each other as possible.

Use analytical techniques that block you from developing preliminary ideas about what your data are telling you. This could include analysing samples out of order, blinding your data or using different statistical tests.

Before looking at your data, try to articulate all possible outcomes, and how you would test and differentiate each one.

Keep in mind that a null result is not a failure but rather an additional piece of information.

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A Kansan's Guide to Science, page 2 of 4 Prev Page--Start || Next Page--History

The Nature of Science

What is science and the scientific method.

Science is a way of learning about the universe. It provides a way of understanding and explaining our observations of the natural world. Science deals with observed phenomena and seeks to explain the way the world works by employing natural, orderly relationships. Science pursues truth within very narrow limits. Some of humanity's most profound questions pertain to meaning, purpose, and morality. Their answers lie beyond the reach of science.

A crucial part of the scientific method involves making hypotheses. A hypothesis (boldface words are included in the glossary at the end of the book) is an idea that explains observations about the world. In most areas of science, a useful hypothesis is one that makes predictions about the results of future experiments or of future observations from the natural world. These can then be tested against new observations and the hypothesis modified or rejected if necessary.

A hypothesis that has been tested repeatedly and never found to be false is likely to be accepted. If it survives many tests and if it covers a range of topics, it may be referred to as a theory. Thus, in science the term theory has a precise meaning, different from its use in everyday life. A scientific theory is "a statement of what are held to be the general laws, principles, or causes of something known or observed" (Oxford English Dictionary). The important thing is that it is testable. Some well-known theories are the theory of gravitation and the atomic theory of matter. An idea--a theory or hypothesis--that cannot be tested is not interesting to scientists. They refer to such ideas that are outside the realm of science as being unfalsifiable. For example, many ideas about religion are unfalsifiable by the methods of science and thus lie outside the realm of science.

A common misunderstanding about the work of scientists has to do with proof. Although hypotheses or theories can be disproved by the methods of science, they cannot be proved. No scientific theory can be proved in the sense of a mathematical or logical proof. Any accepted scientific theory is simply the best existing explanation of the observations already made, an explanation that has not been shown to be false. This is as true for astronomy, geology, and evolutionary biology as it is for the nonhistorical sciences of physics and chemistry. It is important to realize that some theories are held in higher regard than others. Those that explain a wide range of observations and that generate many fruitful and testable hypotheses are held with greater confidence. Evolution is one such theory. It has been incredibly fruitful for research in medicine, developmental biology, genetics, ecology, paleontology, and geology.

Besides being testable and falsifiable, a scientific idea is most useful if it answers more questions than it raises. The idea that the Earth revolves around the sun is a good scientific idea because it explains the progression of the seasons upon which much of our economy is based. No one has ever seen the Earth move around the sun, and no one will ever do so because there can be no stationary platform in space from which to make such observations. Similarly, no one has seen an electron, touched a thermometer to the sun to learn that it is very hot, or watched Tyrannosaurus rex stalk its prey. In spite of this, these scientific ideas are considered useful and reasonable interpretations of the evidence.

The general field of science can be divided into two groups--historical science and experimental science. Experimental science includes physics, chemistry, genetics, and some branches of astronomy. Experimental scientists probably fit the popular conception of scientists as people in white lab coats pouring chemicals or watching dials. Experimental scientists are interested in making predictions about the future. Ultimately, their goal is to derive scientific laws from hypotheses and theories. Laws are statements, such as the law of gravity, that always apply.

Geology, paleontology, evolutionary biology, and some branches of astronomy are said to be historical sciences. A useful way of thinking about those sciences is to realize that the experiments have already been run by nature, sometimes millions of years ago. The job of the historical scientist is to determine what happened in the past--what experiment nature conducted. A hypothesis in the historical sciences is an explanation of these past events, an explanation that can be tested.

A historical scientist can make observations and statements about the past and develop hypotheses from evidence left from nature's past experiments. For example, at numerous times in the distant past the temperature at the Earth's surface was much lower than it is today. At each of these episodes of cooling, we can study how the animal and plant life evolved, migrated, and went extinct. This enables us to say how life has been affected by these events. We can use this information to make statements about past events and predictions of the future.

Hypotheses in the historical sciences are continually being tested by new observations. If the ideas in a hypothesis are contradicted by new observations, then that hypothesis is rejected. Like all sciences, the historical sciences proceed by an almost continual process of hypothesis development and testing. Many ideas about the historical sciences that were developed in the past have been discarded with the accumulation of new observations and the development of new ideas that provide better explanations. Evolutionary theory remains powerful because it continues to generate fruitful and testable hypotheses and has not been contradicted by evidence from the natural world and because it best explains our observations.

Evolutionary biologists, from paleontologists to molecular geneticists, try to determine the precise sequence of life's history and which species are most closely related to one another. Thus, the study of evolution involves historical science. Evolutionary biologists, however, also try to determine the mechanisms that govern how life evolves. These mechanisms include natural selection, chance factors, and various constraints on the way life evolves. That is why the study of evolution also involves experimental science. We can test some of the mechanisms of evolution in the lab by studying bacteria, flies, or mice over many generations and watching how they evolve over months or years in controlled experiments. Many similar natural experiments have been conducted over many millions of years, and their results are recorded in the fossil record of ancient life.

Isn't creation science a type of science?

In spite of its name, creation science is not science at all but is in fact pseudoscience, much like astrology or alchemy. Pseudoscience does not restrict itself to natural explanations of the physical world but often involves supernatural explanations. Creation science is a branch of creationism that is based on the literal interpretation of the Bible and holds that the origin of living species and the Earth occurred as a single event a few thousand years ago. Creation science, unlike legitimate sciences, does not seek to derive explanations through observations and testing of the natural world but instead begins with a belief system and then seeks to find evidence to support this view. Therefore, by its very nature, creation science is not a science because it is not testable and falsifiable.

Understanding Evolution

What is evolution.

Biological evolution is the theory that life on Earth has developed gradually from a common ancestor. This is sometimes described as descent with modification. Since life's origin some 4 billion years ago, life has changed in numerous ways as it branched out into different lineages, with adaptations to different environments and changes in genetic makeup. Some major changes include the evolution of plants and animals and, among the animals, some major groups became extinct. The evolution of life can be thought of as a tree with different lines splitting off. Some branches were cut short by extinction, while others are represented by species living in the modern world. It is inaccurate, for example, to view the evolution of the hominids, as a straight-line transformation from some apelike form into transitional forms and eventually with humans at the pinnacle. Humans did not evolve from chimpanzees or gorillas, but instead humans and modern apes share a common ancestor that lived at some time in the past, probably about 6 million years ago.

Is evolution a fact or a theory?

Evolution is not just a fact and not just a theory; it is both! Evolution is a fact because there is overwhelming scientific evidence that evolution has happened in the past and continues to happen. No reputable scientist questions evolution. That is, all scientific data support the fact that the Earth is more than 4.5 billion years old, that cellular life appeared before 3.5 billion years ago, and that all modern life is descended from a common ancestor. Sometimes people ask scientists and teachers, in the interest of fairness, to present the evidence against evolution. Scientists and teachers do not present such evidence because there simply is no such evidence against evolution. True, there are things we do not yet know, but no one has found any scientific evidence to suggest that evolution does not occur, or that it does not explain all

When people say that evolution is just a theory, they are partly correct; but they are confused about what it means for an idea to be a scientific theory. The scientific usage of theory is different from its meaning in everyday usage. In everyday language, a theory is a hunch or a guess. In science a theory is defined as "a statement of what are held to be the general laws, principles, or causes of something known or observed" (Oxford English Dictionary). Thus, when scientists refer to evolutionary theory, they are talking about the mechanisms of evolution, not about some vague idea of whether evolution has happened or not. Evolutionary theories are a way of explaining the fact of evolution. Scientists have suggested many mechanisms of evolution. Some we accept; some we no longer accept. For example, the theory of natural selection, put forward by Darwin and Wallace in 1858, is still considered an accurate explanation of this mechanism of evolution. On the other hand, we no longer accept the theory of inheritance of acquired characters, which was proposed early in the 19th century by the French biologist Lamarck. Among other things, for example, Lamarck had the idea that successive generations of giraffes inherited ever longer necks because their parents stretched their necks to reach higher branches.

In all sciences, debate about theories exists, but that does not change the facts. For example, just because we cannot always predict where a thunderstorm will happen, this does not mean that thunderstorms do not occur. Similarly, today biologists and paleontologists sometimes debate the importance of different evolutionary mechanisms such as natural selection. These debates do not detract from the fact of evolution; they only demonstrate that evolution is a complex process, like the weather, and that the work of science is not yet finished.

What is the evidence that evolution has actually happened?

The theory of evolution is supported by evidence from experimentation and from the fossil record. In the laboratory, scientists have shown how fruit flies, bacteria, and many other types of organisms adapt and change evolutionarily, even over the short time scales of human experimentation. Similarly, scientists have observed populations evolving in the wild, both in Kansas and elsewhere. The development of resistance to antibiotics by various disease-causing bacteria, including the bacteria responsible for tuberculosis, is one well-known and unfortunate example of evolution.

Fig. 1 --Evolutionary sequence depicting the transition between terrestrial mammals and whales (mya = million years ago). After drawings by N. Haver, copyright Sinauer Associates; in , Purves et al. (1998).

Several Kansas fossils are key transitional species. One example is the Pennsylvanian-aged reptile Petrolacosaurus , which has features of early reptilian groups as well as later reptiles like crocodiles and the dinosaurs (figs. 4 and 5 [ on next page ]). Edaphosaurus (fig. 6 [ on next page ]), also known from a Pennsylvanian-aged lagoon in Kansas, is a transitional link between early reptiles and other groups more closely related to modern mammals. The mosasaurs, which must have terrified the occupants of Kansas seaways in the Cretaceous, are a link between lizardlike groups and snakes (fig. 8 [ on next page ]). The Cretaceous-aged Parahesperornis , an early bird, is another important evolutionary transitional form between reptiles and modern birds (fig. 11 [ on next page ]). Like extinct and modern reptiles, Parahesperornis had bones that were not hollow and a mouth filled with teeth, yet it had a birdlike skull, wings, feet, and body, making it a transitional form. Finally, a more recent example is Pliohippus , a relative of the modern horse that roamed the grassy plains of Kansas within the last few million years. Like the modern horse that served the native peoples and early settlers of Kansas so well, Pliohippus was well adapted to the ancient environments of Kansas. About the size of a modern pony, Pliohippus had a single toe or hoof, and is a transitional link between the older, small horses with many toes, which are extinct and only known as fossils, and the modern large draft horse that populates Kansas.

What is the difference between microevolution and macroevolution?

Many people wonder whether the small-scale evolution that scientists observe in the laboratory and in the wild ( microevolution ) is distinct from the large-scale evolutionary trends that scientists observe, say, in the fossil record ( macroevolution ). What seems to separate microevolution from macroevolution is the act of speciation--that is, the process in which a new species is formed. Changes can occur within a single species as it adapts to its local environment; changes that are referred to as microevolution. Antibiotic resistance in bacteria is a good example of microevolutionary change. In adapting to an environment of antibiotics through natural selection, bacteria have evolved resistance to the drugs. If there is microevolutionary change within a species but that species does not split off a new descendent species, then there is unlikely to be any visible evolutionary change over long time scales.

Major or macroevolutionary changes are tied to speciation, the origin of new species, which usually occurs when populations of a species are isolated from one another over long periods of time. A small, isolated population can then undergo its own independent microevolutionary changes as it adapts to local environments. With sufficient evolutionary divergence, a new species evolves, and microevolutionary changes are translated to macroevolution. Thus, microevolution and macroevolution are not distinct processes. They are related, and macroevolution results when such situations as the geographical isolation of small populations occur. This makes it more likely that microevolutionary changes will add up to macroevolution.

The evolution of horses provides an example of how new species form following geographic isolation. North America was home to the earliest known horse, Hyracotherium , which was about the size of a small dog, with a curved back, unspecialized teeth, and three and four toes on the back and front hooves respectively, whereas modern horses have only one toe on each of their four hoofs. In the Miocene, about 35 million years ago, as extensive grassy prairies evolved for the first time, Merychippus , a three-toed horse that descended from Hyracotherium , evolved high-crowned teeth that allowed it to feed on grass. Its large size enabled it to run fast to escape the predators that were evolving at the time, especially sabertoothed cats and wolves.

Descendants of Merychippus migrated from North America to the Old World where, in isolated populations, new species evolved that soon went extinct. Meanwhile, in North America isolated populations of Merychippus evolved into the one-toed Pliohippus in the Pliocene and into Equus in the Pleistocene. Shortly after early humans came to North America, Equus went extinct on this continent, but the populations of Equus that had migrated to the Old World thrived and again, as isolated populations, evolved into the six species of Equus we know today as well as several that are known only from the fossil record. Today's species include three separate species of zebra, the onager of Asia, the donkey, and Equus caballus , the modern horse. Domestication of the modern horse began some 2,500 years ago, and they were taken by humans to North America some time around 1500 AD.

What is natural selection?

Natural selection happens when some individuals in a population survive and reproduce more successfully than others because of their inherited traits. Because these traits enable the individuals to reproduce more successfully, over time the traits will appear more frequently and begin to accumulate in the population. The outcome of this microevolutionary process is adaptation . That is, over time through natural selection, individuals in a population will come to possess traits that lead to their being well adapted to their environment. In fact, Darwin's ideas on how natural selection works are really rather simple, and they are exactly the same as the procedure animal breeders have used for thousands of years to develop new breeds of animals. Animals vary, and some of the variation is inherited. Animals have more offspring than can possibly survive. On average, other things being equal, animals will tend to survive if they have inherited traits that are favored by the environment to lead to survival and reproduction. In this way, favorable, adaptive traits accumulate in a population of organisms, and traits that are not adaptive are weeded out. Such principles are being applied today to Kansas livestock and crop species.

Is natural selection the best explanation of evolution?

Natural selection is often described as the best explanation of how evolution happened, and numerous examples demonstrate that it is an important force controlling evolution. Polar bears offer one example. They hunt in the snowy arctic; they sneak up on their prey; and they are, after all, white. Thus they appear to be well adapted to their environment. Many features in the history of life, however, suggest that chance has played a very important role in evolution, too. For example, several times in the history of life, mass extinctions have occurred--events so severe that they eliminated most of the species on Earth. Such a mass extinction ended the dinosaurs' reign at the end of the Cretaceous Period. Prior to the mass extinction, dinosaurs were large and dominated the world, whereas mammals were small and nocturnal, sneaking around in the dinosaurs' world. It was not until the dinosaurs went extinct that the mammals could take over and flourish. The mechanism that caused this extinction was a massive asteroid striking the Earth. This example points out that natural selection alone is insufficient to explain the history of life. No matter how well adapted an animal is, it may not survive a catastrophe like a collision with a five-mile-wide asteroid.

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