example of hypothesis with two independent variables

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8.2 Multiple Independent Variables

Learning objectives.

• Explain why researchers often include multiple independent variables in their studies.
• Define factorial design, and use a factorial design table to represent and interpret simple factorial designs.
• Distinguish between main effects and interactions, and recognize and give examples of each.
• Sketch and interpret bar graphs and line graphs showing the results of studies with simple factorial designs.

Just as it is common for studies in psychology to include multiple dependent variables, it is also common for them to include multiple independent variables. Schnall and her colleagues studied the effect of both disgust and private body consciousness in the same study. Researchers’ inclusion of multiple independent variables in one experiment is further illustrated by the following actual titles from various professional journals:

• The Effects of Temporal Delay and Orientation on Haptic Object Recognition
• Opening Closed Minds: The Combined Effects of Intergroup Contact and Need for Closure on Prejudice
• Effects of Expectancies and Coping on Pain-Induced Intentions to Smoke
• The Effect of Age and Divided Attention on Spontaneous Recognition
• The Effects of Reduced Food Size and Package Size on the Consumption Behavior of Restrained and Unrestrained Eaters

Just as including multiple dependent variables in the same experiment allows one to answer more research questions, so too does including multiple independent variables in the same experiment. For example, instead of conducting one study on the effect of disgust on moral judgment and another on the effect of private body consciousness on moral judgment, Schnall and colleagues were able to conduct one study that addressed both questions. But including multiple independent variables also allows the researcher to answer questions about whether the effect of one independent variable depends on the level of another. This is referred to as an interaction between the independent variables. Schnall and her colleagues, for example, observed an interaction between disgust and private body consciousness because the effect of disgust depended on whether participants were high or low in private body consciousness. As we will see, interactions are often among the most interesting results in psychological research.

Factorial Designs

By far the most common approach to including multiple independent variables in an experiment is the factorial design. In a factorial design , each level of one independent variable (which can also be called a factor ) is combined with each level of the others to produce all possible combinations. Each combination, then, becomes a condition in the experiment. Imagine, for example, an experiment on the effect of cell phone use (yes vs. no) and time of day (day vs. night) on driving ability. This is shown in the factorial design table in Figure 8.2 “Factorial Design Table Representing a 2 × 2 Factorial Design” . The columns of the table represent cell phone use, and the rows represent time of day. The four cells of the table represent the four possible combinations or conditions: using a cell phone during the day, not using a cell phone during the day, using a cell phone at night, and not using a cell phone at night. This particular design is a 2 × 2 (read “two-by-two”) factorial design because it combines two variables, each of which has two levels. If one of the independent variables had a third level (e.g., using a handheld cell phone, using a hands-free cell phone, and not using a cell phone), then it would be a 3 × 2 factorial design, and there would be six distinct conditions. Notice that the number of possible conditions is the product of the numbers of levels. A 2 × 2 factorial design has four conditions, a 3 × 2 factorial design has six conditions, a 4 × 5 factorial design would have 20 conditions, and so on.

Figure 8.2 Factorial Design Table Representing a 2 × 2 Factorial Design

In principle, factorial designs can include any number of independent variables with any number of levels. For example, an experiment could include the type of psychotherapy (cognitive vs. behavioral), the length of the psychotherapy (2 weeks vs. 2 months), and the sex of the psychotherapist (female vs. male). This would be a 2 × 2 × 2 factorial design and would have eight conditions. Figure 8.3 “Factorial Design Table Representing a 2 × 2 × 2 Factorial Design” shows one way to represent this design. In practice, it is unusual for there to be more than three independent variables with more than two or three levels each because the number of conditions can quickly become unmanageable. For example, adding a fourth independent variable with three levels (e.g., therapist experience: low vs. medium vs. high) to the current example would make it a 2 × 2 × 2 × 3 factorial design with 24 distinct conditions. In the rest of this section, we will focus on designs with two independent variables. The general principles discussed here extend in a straightforward way to more complex factorial designs.

Figure 8.3 Factorial Design Table Representing a 2 × 2 × 2 Factorial Design

Assigning Participants to Conditions

Recall that in a simple between-subjects design, each participant is tested in only one condition. In a simple within-subjects design, each participant is tested in all conditions. In a factorial experiment, the decision to take the between-subjects or within-subjects approach must be made separately for each independent variable. In a between-subjects factorial design , all of the independent variables are manipulated between subjects. For example, all participants could be tested either while using a cell phone or while not using a cell phone and either during the day or during the night. This would mean that each participant was tested in one and only one condition. In a within-subjects factorial design , all of the independent variables are manipulated within subjects. All participants could be tested both while using a cell phone and while not using a cell phone and both during the day and during the night. This would mean that each participant was tested in all conditions. The advantages and disadvantages of these two approaches are the same as those discussed in Chapter 6 “Experimental Research” . The between-subjects design is conceptually simpler, avoids carryover effects, and minimizes the time and effort of each participant. The within-subjects design is more efficient for the researcher and controls extraneous participant variables.

It is also possible to manipulate one independent variable between subjects and another within subjects. This is called a mixed factorial design . For example, a researcher might choose to treat cell phone use as a within-subjects factor by testing the same participants both while using a cell phone and while not using a cell phone (while counterbalancing the order of these two conditions). But he or she might choose to treat time of day as a between-subjects factor by testing each participant either during the day or during the night (perhaps because this only requires them to come in for testing once). Thus each participant in this mixed design would be tested in two of the four conditions.

Regardless of whether the design is between subjects, within subjects, or mixed, the actual assignment of participants to conditions or orders of conditions is typically done randomly.

Nonmanipulated Independent Variables

In many factorial designs, one of the independent variables is a nonmanipulated independent variable . The researcher measures it but does not manipulate it. The study by Schnall and colleagues is a good example. One independent variable was disgust, which the researchers manipulated by testing participants in a clean room or a messy room. The other was private body consciousness, which the researchers simply measured. Another example is a study by Halle Brown and colleagues in which participants were exposed to several words that they were later asked to recall (Brown, Kosslyn, Delamater, Fama, & Barsky, 1999). The manipulated independent variable was the type of word. Some were negative health-related words (e.g., tumor , coronary ), and others were not health related (e.g., election , geometry ). The nonmanipulated independent variable was whether participants were high or low in hypochondriasis (excessive concern with ordinary bodily symptoms). The result of this study was that the participants high in hypochondriasis were better than those low in hypochondriasis at recalling the health-related words, but they were no better at recalling the non-health-related words.

Such studies are extremely common, and there are several points worth making about them. First, nonmanipulated independent variables are usually participant variables (private body consciousness, hypochondriasis, self-esteem, and so on), and as such they are by definition between-subjects factors. For example, people are either low in hypochondriasis or high in hypochondriasis; they cannot be tested in both of these conditions. Second, such studies are generally considered to be experiments as long as at least one independent variable is manipulated, regardless of how many nonmanipulated independent variables are included. Third, it is important to remember that causal conclusions can only be drawn about the manipulated independent variable. For example, Schnall and her colleagues were justified in concluding that disgust affected the harshness of their participants’ moral judgments because they manipulated that variable and randomly assigned participants to the clean or messy room. But they would not have been justified in concluding that participants’ private body consciousness affected the harshness of their participants’ moral judgments because they did not manipulate that variable. It could be, for example, that having a strict moral code and a heightened awareness of one’s body are both caused by some third variable (e.g., neuroticism). Thus it is important to be aware of which variables in a study are manipulated and which are not.

Graphing the Results of Factorial Experiments

The results of factorial experiments with two independent variables can be graphed by representing one independent variable on the x- axis and representing the other by using different kinds of bars or lines. (The y- axis is always reserved for the dependent variable.) Figure 8.4 “Two Ways to Plot the Results of a Factorial Experiment With Two Independent Variables” shows results for two hypothetical factorial experiments. The top panel shows the results of a 2 × 2 design. Time of day (day vs. night) is represented by different locations on the x- axis, and cell phone use (no vs. yes) is represented by different-colored bars. (It would also be possible to represent cell phone use on the x- axis and time of day as different-colored bars. The choice comes down to which way seems to communicate the results most clearly.) The bottom panel of Figure 8.4 “Two Ways to Plot the Results of a Factorial Experiment With Two Independent Variables” shows the results of a 4 × 2 design in which one of the variables is quantitative. This variable, psychotherapy length, is represented along the x- axis, and the other variable (psychotherapy type) is represented by differently formatted lines. This is a line graph rather than a bar graph because the variable on the x- axis is quantitative with a small number of distinct levels.

Figure 8.4 Two Ways to Plot the Results of a Factorial Experiment With Two Independent Variables

Main Effects and Interactions

In factorial designs, there are two kinds of results that are of interest: main effects and interaction effects (which are also called just “interactions”). A main effect is the statistical relationship between one independent variable and a dependent variable—averaging across the levels of the other independent variable. Thus there is one main effect to consider for each independent variable in the study. The top panel of Figure 8.4 “Two Ways to Plot the Results of a Factorial Experiment With Two Independent Variables” shows a main effect of cell phone use because driving performance was better, on average, when participants were not using cell phones than when they were. The blue bars are, on average, higher than the red bars. It also shows a main effect of time of day because driving performance was better during the day than during the night—both when participants were using cell phones and when they were not. Main effects are independent of each other in the sense that whether or not there is a main effect of one independent variable says nothing about whether or not there is a main effect of the other. The bottom panel of Figure 8.4 “Two Ways to Plot the Results of a Factorial Experiment With Two Independent Variables” , for example, shows a clear main effect of psychotherapy length. The longer the psychotherapy, the better it worked. But it also shows no overall advantage of one type of psychotherapy over the other.

There is an interaction effect (or just “interaction”) when the effect of one independent variable depends on the level of another. Although this might seem complicated, you have an intuitive understanding of interactions already. It probably would not surprise you, for example, to hear that the effect of receiving psychotherapy is stronger among people who are highly motivated to change than among people who are not motivated to change. This is an interaction because the effect of one independent variable (whether or not one receives psychotherapy) depends on the level of another (motivation to change). Schnall and her colleagues also demonstrated an interaction because the effect of whether the room was clean or messy on participants’ moral judgments depended on whether the participants were low or high in private body consciousness. If they were high in private body consciousness, then those in the messy room made harsher judgments. If they were low in private body consciousness, then whether the room was clean or messy did not matter.

The effect of one independent variable can depend on the level of the other in different ways. This is shown in Figure 8.5 “Bar Graphs Showing Three Types of Interactions” . In the top panel, one independent variable has an effect at one level of the second independent variable but no effect at the others. (This is much like the study of Schnall and her colleagues where there was an effect of disgust for those high in private body consciousness but not for those low in private body consciousness.) In the middle panel, one independent variable has a stronger effect at one level of the second independent variable than at the other level. This is like the hypothetical driving example where there was a stronger effect of using a cell phone at night than during the day. In the bottom panel, one independent variable again has an effect at both levels of the second independent variable, but the effects are in opposite directions. Figure 8.5 “Bar Graphs Showing Three Types of Interactions” shows the strongest form of this kind of interaction, called a crossover interaction . One example of a crossover interaction comes from a study by Kathy Gilliland on the effect of caffeine on the verbal test scores of introverts and extroverts (Gilliland, 1980). Introverts perform better than extroverts when they have not ingested any caffeine. But extroverts perform better than introverts when they have ingested 4 mg of caffeine per kilogram of body weight. Figure 8.6 “Line Graphs Showing Three Types of Interactions” shows examples of these same kinds of interactions when one of the independent variables is quantitative and the results are plotted in a line graph. Note that in a crossover interaction, the two lines literally “cross over” each other.

Figure 8.5 Bar Graphs Showing Three Types of Interactions

In the top panel, one independent variable has an effect at one level of the second independent variable but not at the other. In the middle panel, one independent variable has a stronger effect at one level of the second independent variable than at the other. In the bottom panel, one independent variable has the opposite effect at one level of the second independent variable than at the other.

Figure 8.6 Line Graphs Showing Three Types of Interactions

In many studies, the primary research question is about an interaction. The study by Brown and her colleagues was inspired by the idea that people with hypochondriasis are especially attentive to any negative health-related information. This led to the hypothesis that people high in hypochondriasis would recall negative health-related words more accurately than people low in hypochondriasis but recall non-health-related words about the same as people low in hypochondriasis. And of course this is exactly what happened in this study.

Key Takeaways

• Researchers often include multiple independent variables in their experiments. The most common approach is the factorial design, in which each level of one independent variable is combined with each level of the others to create all possible conditions.
• In a factorial design, the main effect of an independent variable is its overall effect averaged across all other independent variables. There is one main effect for each independent variable.
• There is an interaction between two independent variables when the effect of one depends on the level of the other. Some of the most interesting research questions and results in psychology are specifically about interactions.
• Practice: Return to the five article titles presented at the beginning of this section. For each one, identify the independent variables and the dependent variable.
• Practice: Create a factorial design table for an experiment on the effects of room temperature and noise level on performance on the SAT. Be sure to indicate whether each independent variable will be manipulated between subjects or within subjects and explain why.

Brown, H. D., Kosslyn, S. M., Delamater, B., Fama, A., & Barsky, A. J. (1999). Perceptual and memory biases for health-related information in hypochondriacal individuals. Journal of Psychosomatic Research , 47 , 67–78.

Gilliland, K. (1980). The interactive effect of introversion-extroversion with caffeine induced arousal on verbal performance. Journal of Research in Personality , 14 , 482–492.

Research Methods in Psychology Copyright © 2016 by University of Minnesota is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License , except where otherwise noted.

Research Hypothesis In Psychology: Types, & Examples

Saul Mcleod, PhD

Editor-in-Chief for Simply Psychology

BSc (Hons) Psychology, MRes, PhD, University of Manchester

Saul Mcleod, PhD., is a qualified psychology teacher with over 18 years of experience in further and higher education. He has been published in peer-reviewed journals, including the Journal of Clinical Psychology.

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Olivia Guy-Evans, MSc

Associate Editor for Simply Psychology

BSc (Hons) Psychology, MSc Psychology of Education

Olivia Guy-Evans is a writer and associate editor for Simply Psychology. She has previously worked in healthcare and educational sectors.

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A research hypothesis, in its plural form “hypotheses,” is a specific, testable prediction about the anticipated results of a study, established at its outset. It is a key component of the scientific method .

Hypotheses connect theory to data and guide the research process towards expanding scientific understanding

Some key points about hypotheses:

• A hypothesis expresses an expected pattern or relationship. It connects the variables under investigation.
• It is stated in clear, precise terms before any data collection or analysis occurs. This makes the hypothesis testable.
• A hypothesis must be falsifiable. It should be possible, even if unlikely in practice, to collect data that disconfirms rather than supports the hypothesis.
• Hypotheses guide research. Scientists design studies to explicitly evaluate hypotheses about how nature works.
• For a hypothesis to be valid, it must be testable against empirical evidence. The evidence can then confirm or disprove the testable predictions.
• Hypotheses are informed by background knowledge and observation, but go beyond what is already known to propose an explanation of how or why something occurs.

Predictions typically arise from a thorough knowledge of the research literature, curiosity about real-world problems or implications, and integrating this to advance theory. They build on existing literature while providing new insight.

Types of Research Hypotheses

Alternative hypothesis.

The research hypothesis is often called the alternative or experimental hypothesis in experimental research.

It typically suggests a potential relationship between two key variables: the independent variable, which the researcher manipulates, and the dependent variable, which is measured based on those changes.

The alternative hypothesis states a relationship exists between the two variables being studied (one variable affects the other).

A hypothesis is a testable statement or prediction about the relationship between two or more variables. It is a key component of the scientific method. Some key points about hypotheses:

• Important hypotheses lead to predictions that can be tested empirically. The evidence can then confirm or disprove the testable predictions.

In summary, a hypothesis is a precise, testable statement of what researchers expect to happen in a study and why. Hypotheses connect theory to data and guide the research process towards expanding scientific understanding.

An experimental hypothesis predicts what change(s) will occur in the dependent variable when the independent variable is manipulated.

It states that the results are not due to chance and are significant in supporting the theory being investigated.

The alternative hypothesis can be directional, indicating a specific direction of the effect, or non-directional, suggesting a difference without specifying its nature. It’s what researchers aim to support or demonstrate through their study.

Null Hypothesis

The null hypothesis states no relationship exists between the two variables being studied (one variable does not affect the other). There will be no changes in the dependent variable due to manipulating the independent variable.

It states results are due to chance and are not significant in supporting the idea being investigated.

The null hypothesis, positing no effect or relationship, is a foundational contrast to the research hypothesis in scientific inquiry. It establishes a baseline for statistical testing, promoting objectivity by initiating research from a neutral stance.

Many statistical methods are tailored to test the null hypothesis, determining the likelihood of observed results if no true effect exists.

This dual-hypothesis approach provides clarity, ensuring that research intentions are explicit, and fosters consistency across scientific studies, enhancing the standardization and interpretability of research outcomes.

Nondirectional Hypothesis

A non-directional hypothesis, also known as a two-tailed hypothesis, predicts that there is a difference or relationship between two variables but does not specify the direction of this relationship.

It merely indicates that a change or effect will occur without predicting which group will have higher or lower values.

For example, “There is a difference in performance between Group A and Group B” is a non-directional hypothesis.

Directional Hypothesis

A directional (one-tailed) hypothesis predicts the nature of the effect of the independent variable on the dependent variable. It predicts in which direction the change will take place. (i.e., greater, smaller, less, more)

It specifies whether one variable is greater, lesser, or different from another, rather than just indicating that there’s a difference without specifying its nature.

For example, “Exercise increases weight loss” is a directional hypothesis.

Falsifiability

The Falsification Principle, proposed by Karl Popper , is a way of demarcating science from non-science. It suggests that for a theory or hypothesis to be considered scientific, it must be testable and irrefutable.

Falsifiability emphasizes that scientific claims shouldn’t just be confirmable but should also have the potential to be proven wrong.

It means that there should exist some potential evidence or experiment that could prove the proposition false.

However many confirming instances exist for a theory, it only takes one counter observation to falsify it. For example, the hypothesis that “all swans are white,” can be falsified by observing a black swan.

For Popper, science should attempt to disprove a theory rather than attempt to continually provide evidence to support a research hypothesis.

Can a Hypothesis be Proven?

Hypotheses make probabilistic predictions. They state the expected outcome if a particular relationship exists. However, a study result supporting a hypothesis does not definitively prove it is true.

All studies have limitations. There may be unknown confounding factors or issues that limit the certainty of conclusions. Additional studies may yield different results.

In science, hypotheses can realistically only be supported with some degree of confidence, not proven. The process of science is to incrementally accumulate evidence for and against hypothesized relationships in an ongoing pursuit of better models and explanations that best fit the empirical data. But hypotheses remain open to revision and rejection if that is where the evidence leads.

• Disproving a hypothesis is definitive. Solid disconfirmatory evidence will falsify a hypothesis and require altering or discarding it based on the evidence.
• However, confirming evidence is always open to revision. Other explanations may account for the same results, and additional or contradictory evidence may emerge over time.

We can never 100% prove the alternative hypothesis. Instead, we see if we can disprove, or reject the null hypothesis.

If we reject the null hypothesis, this doesn’t mean that our alternative hypothesis is correct but does support the alternative/experimental hypothesis.

Upon analysis of the results, an alternative hypothesis can be rejected or supported, but it can never be proven to be correct. We must avoid any reference to results proving a theory as this implies 100% certainty, and there is always a chance that evidence may exist which could refute a theory.

How to Write a Hypothesis

• Identify variables . The researcher manipulates the independent variable and the dependent variable is the measured outcome.
• Operationalized the variables being investigated . Operationalization of a hypothesis refers to the process of making the variables physically measurable or testable, e.g. if you are about to study aggression, you might count the number of punches given by participants.
• Decide on a direction for your prediction . If there is evidence in the literature to support a specific effect of the independent variable on the dependent variable, write a directional (one-tailed) hypothesis. If there are limited or ambiguous findings in the literature regarding the effect of the independent variable on the dependent variable, write a non-directional (two-tailed) hypothesis.
• Make it Testable : Ensure your hypothesis can be tested through experimentation or observation. It should be possible to prove it false (principle of falsifiability).
• Clear & concise language . A strong hypothesis is concise (typically one to two sentences long), and formulated using clear and straightforward language, ensuring it’s easily understood and testable.

Consider a hypothesis many teachers might subscribe to: students work better on Monday morning than on Friday afternoon (IV=Day, DV= Standard of work).

Now, if we decide to study this by giving the same group of students a lesson on a Monday morning and a Friday afternoon and then measuring their immediate recall of the material covered in each session, we would end up with the following:

• The alternative hypothesis states that students will recall significantly more information on a Monday morning than on a Friday afternoon.
• The null hypothesis states that there will be no significant difference in the amount recalled on a Monday morning compared to a Friday afternoon. Any difference will be due to chance or confounding factors.

More Examples

• Memory : Participants exposed to classical music during study sessions will recall more items from a list than those who studied in silence.
• Social Psychology : Individuals who frequently engage in social media use will report higher levels of perceived social isolation compared to those who use it infrequently.
• Developmental Psychology : Children who engage in regular imaginative play have better problem-solving skills than those who don’t.
• Clinical Psychology : Cognitive-behavioral therapy will be more effective in reducing symptoms of anxiety over a 6-month period compared to traditional talk therapy.
• Cognitive Psychology : Individuals who multitask between various electronic devices will have shorter attention spans on focused tasks than those who single-task.
• Health Psychology : Patients who practice mindfulness meditation will experience lower levels of chronic pain compared to those who don’t meditate.
• Organizational Psychology : Employees in open-plan offices will report higher levels of stress than those in private offices.
• Behavioral Psychology : Rats rewarded with food after pressing a lever will press it more frequently than rats who receive no reward.

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• How to Write a Strong Hypothesis | Guide & Examples

How to Write a Strong Hypothesis | Guide & Examples

Published on 6 May 2022 by Shona McCombes .

A hypothesis is a statement that can be tested by scientific research. If you want to test a relationship between two or more variables, you need to write hypotheses before you start your experiment or data collection.

Table of contents

What is a hypothesis, developing a hypothesis (with example), hypothesis examples, frequently asked questions about writing hypotheses.

A hypothesis states your predictions about what your research will find. It is a tentative answer to your research question that has not yet been tested. For some research projects, you might have to write several hypotheses that address different aspects of your research question.

A hypothesis is not just a guess – it should be based on existing theories and knowledge. It also has to be testable, which means you can support or refute it through scientific research methods (such as experiments, observations, and statistical analysis of data).

Variables in hypotheses

Hypotheses propose a relationship between two or more variables . An independent variable is something the researcher changes or controls. A dependent variable is something the researcher observes and measures.

In this example, the independent variable is exposure to the sun – the assumed cause . The dependent variable is the level of happiness – the assumed effect .

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Step 1: ask a question.

Writing a hypothesis begins with a research question that you want to answer. The question should be focused, specific, and researchable within the constraints of your project.

Step 2: Do some preliminary research

Your initial answer to the question should be based on what is already known about the topic. Look for theories and previous studies to help you form educated assumptions about what your research will find.

At this stage, you might construct a conceptual framework to identify which variables you will study and what you think the relationships are between them. Sometimes, you’ll have to operationalise more complex constructs.

Step 3: Formulate your hypothesis

Now you should have some idea of what you expect to find. Write your initial answer to the question in a clear, concise sentence.

Step 4: Refine your hypothesis

You need to make sure your hypothesis is specific and testable. There are various ways of phrasing a hypothesis, but all the terms you use should have clear definitions, and the hypothesis should contain:

• The relevant variables
• The specific group being studied
• The predicted outcome of the experiment or analysis

Step 5: Phrase your hypothesis in three ways

To identify the variables, you can write a simple prediction in if … then form. The first part of the sentence states the independent variable and the second part states the dependent variable.

In academic research, hypotheses are more commonly phrased in terms of correlations or effects, where you directly state the predicted relationship between variables.

If you are comparing two groups, the hypothesis can state what difference you expect to find between them.

Step 6. Write a null hypothesis

If your research involves statistical hypothesis testing , you will also have to write a null hypothesis. The null hypothesis is the default position that there is no association between the variables. The null hypothesis is written as H 0 , while the alternative hypothesis is H 1 or H a .

Hypothesis testing is a formal procedure for investigating our ideas about the world using statistics. It is used by scientists to test specific predictions, called hypotheses , by calculating how likely it is that a pattern or relationship between variables could have arisen by chance.

A hypothesis is not just a guess. It should be based on existing theories and knowledge. It also has to be testable, which means you can support or refute it through scientific research methods (such as experiments, observations, and statistical analysis of data).

A research hypothesis is your proposed answer to your research question. The research hypothesis usually includes an explanation (‘ x affects y because …’).

A statistical hypothesis, on the other hand, is a mathematical statement about a population parameter. Statistical hypotheses always come in pairs: the null and alternative hypotheses. In a well-designed study , the statistical hypotheses correspond logically to the research hypothesis.

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Chapter 8: Complex Research Designs

Multiple Independent Variables

Learning Objectives

• Explain why researchers often include multiple independent variables in their studies.
• Define factorial design, and use a factorial design table to represent and interpret simple factorial designs.
• Distinguish between main effects and interactions, and recognize and give examples of each.
• Sketch and interpret bar graphs and line graphs showing the results of studies with simple factorial designs.

Just as it is common for studies in psychology to include multiple dependent variables, it is also common for them to include multiple independent variables. Schnall and her colleagues studied the effect of both disgust and private body consciousness in the same study. Researchers’ inclusion of multiple independent variables in one experiment is further illustrated by the following actual titles from various professional journals:

• The Effects of Temporal Delay and Orientation on Haptic Object Recognition
• Opening Closed Minds: The Combined Effects of Intergroup Contact and Need for Closure on Prejudice
• Effects of Expectancies and Coping on Pain-Induced Intentions to Smoke
• The Effect of Age and Divided Attention on Spontaneous Recognition
• The Effects of Reduced Food Size and Package Size on the Consumption Behaviour of Restrained and Unrestrained Eaters

Just as including multiple dependent variables in the same experiment allows one to answer more research questions, so too does including multiple independent variables in the same experiment. For example, instead of conducting one study on the effect of disgust on moral judgment and another on the effect of private body consciousness on moral judgment, Schnall and colleagues were able to conduct one study that addressed both questions. But including multiple independent variables also allows the researcher to answer questions about whether the effect of one independent variable depends on the level of another. This is referred to as an interaction between the independent variables. Schnall and her colleagues, for example, observed an interaction between disgust and private body consciousness because the effect of disgust depended on whether participants were high or low in private body consciousness. As we will see, interactions are often among the most interesting results in psychological research.

Factorial Designs

By far the most common approach to including multiple independent variables in an experiment is the factorial design. In a  factorial design , each level of one independent variable (which can also be called a  factor ) is combined with each level of the others to produce all possible combinations. Each combination, then, becomes a condition in the experiment. Imagine, for example, an experiment on the effect of cell phone use (yes vs. no) and time of day (day vs. night) on driving ability. This is shown in the  factorial design table  in Figure 8.1. The columns of the table represent cell phone use, and the rows represent time of day. The four cells of the table represent the four possible combinations or conditions: using a cell phone during the day, not using a cell phone during the day, using a cell phone at night, and not using a cell phone at night. This particular design is referred to as a 2 × 2 (read “two-by-two”) factorial design because it combines two variables, each of which has two levels. If one of the independent variables had a third level (e.g., using a handheld cell phone, using a hands-free cell phone, and not using a cell phone), then it would be a 3 × 2 factorial design, and there would be six distinct conditions. Notice that the number of possible conditions is the product of the numbers of levels. A 2 × 2 factorial design has four conditions, a 3 × 2 factorial design has six conditions, a 4 × 5 factorial design would have 20 conditions, and so on.

In principle, factorial designs can include any number of independent variables with any number of levels. For example, an experiment could include the type of psychotherapy (cognitive vs. behavioural), the length of the psychotherapy (2 weeks vs. 2 months), and the sex of the psychotherapist (female vs. male). This would be a 2 × 2 × 2 factorial design and would have eight conditions. Figure 8.2 shows one way to represent this design. In practice, it is unusual for there to be more than three independent variables with more than two or three levels each. This is for at least two reasons: For one, the number of conditions can quickly become unmanageable. For example, adding a fourth independent variable with three levels (e.g., therapist experience: low vs. medium vs. high) to the current example would make it a 2 × 2 × 2 × 3 factorial design with 24 distinct conditions. Second, the number of participants required to populate all of these conditions (while maintaining a reasonable ability to detect a real underlying effect) can render the design unfeasible (for more information, see the discussion about the importance of adequate statistical power in Chapter 13). As a result, in the remainder of this section we will focus on designs with two independent variables. The general principles discussed here extend in a straightforward way to more complex factorial designs.

Assigning Participants to Conditions

Recall that in a simple between-subjects design, each participant is tested in only one condition. In a simple within-subjects design, each participant is tested in all conditions. In a factorial experiment, the decision to take the between-subjects or within-subjects approach must be made separately for each independent variable. In a  between-subjects factorial design , all of the independent variables are manipulated between subjects. For example, all participants could be tested either while using a cell phone  or  while not using a cell phone and either during the day  or  during the night. This would mean that each participant was tested in one and only one condition. In a within-subjects factorial design, all of the independent variables are manipulated within subjects. All participants could be tested both while using a cell phone and  while not using a cell phone and both during the day  and  during the night. This would mean that each participant was tested in all conditions. The advantages and disadvantages of these two approaches are the same as those discussed in  Chapter 6 . The between-subjects design is conceptually simpler, avoids carryover effects, and minimizes the time and effort of each participant. The within-subjects design is more efficient for the researcher and controls extraneous participant variables.

It is also possible to manipulate one independent variable between subjects and another within subjects. This is called a  mixed factorial design . For example, a researcher might choose to treat cell phone use as a within-subjects factor by testing the same participants both while using a cell phone and while not using a cell phone (while counterbalancing the order of these two conditions). But he or she might choose to treat time of day as a between-subjects factor by testing each participant either during the day or during the night (perhaps because this only requires them to come in for testing once). Thus each participant in this mixed design would be tested in two of the four conditions.

Regardless of whether the design is between subjects, within subjects, or mixed, the actual assignment of participants to conditions or orders of conditions is typically done randomly.

Nonmanipulated Independent Variables

In many factorial designs, one of the independent variables is a nonmanipulated independent variable . The researcher measures it but does not manipulate it. The study by Schnall and colleagues is a good example. One independent variable was disgust, which the researchers manipulated by testing participants in a clean room or a messy room. The other was private body consciousness, a participant variable which the researchers simply measured. Another example is a study by Halle Brown and colleagues in which participants were exposed to several words that they were later asked to recall (Brown, Kosslyn, Delamater, Fama, & Barsky, 1999) [1] . The manipulated independent variable was the type of word. Some were negative health-related words (e.g.,  tumor, coronary ), and others were not health related (e.g.,  election, geometry ). The nonmanipulated independent variable was whether participants were high or low in hypochondriasis (excessive concern with ordinary bodily symptoms). The result of this study was that the participants high in hypochondriasis were better than those low in hypochondriasis at recalling the health-related words, but they were no better at recalling the non-health-related words.

Such studies are extremely common, and there are several points worth making about them. First, nonmanipulated independent variables are usually participant variables (private body consciousness, hypochondriasis, self-esteem, and so on), and as such they are by definition between-subjects factors. For example, people are either low in hypochondriasis or high in hypochondriasis; they cannot be tested in both of these conditions. Second, such studies are generally considered to be experiments as long as at least one independent variable is manipulated, regardless of how many nonmanipulated independent variables are included. Third, it is important to remember that causal conclusions can only be drawn about the manipulated independent variable. For example, Schnall and her colleagues were justified in concluding that disgust affected the harshness of their participants’ moral judgments because they manipulated that variable and randomly assigned participants to the clean or messy room. But they would not have been justified in concluding that participants’ private body consciousness affected the harshness of their participants’ moral judgments because they did not manipulate that variable. It could be, for example, that having a strict moral code and a heightened awareness of one’s body are both caused by some third variable (e.g., neuroticism). Thus it is important to be aware of which variables in a study are manipulated and which are not.

Graphing the Results of Factorial Experiments

The results of factorial experiments with two independent variables can be graphed by representing one independent variable on the  x -axis and representing the other by using different kinds of bars or lines. (The  y -axis is always reserved for the dependent variable.) Figure 8.3 shows results for two hypothetical factorial experiments. The top panel shows the results of a 2 × 2 design. Time of day (day vs. night) is represented by different locations on the  x -axis, and cell phone use (no vs. yes) is represented by different-coloured bars. (It would also be possible to represent cell phone use on the  x -axis and time of day as different-coloured bars. The choice comes down to which way seems to communicate the results most clearly.) The bottom panel of Figure 8.3 shows the results of a 4 × 2 design in which one of the variables is quantitative. This variable, psychotherapy length, is represented along the  x -axis, and the other variable (psychotherapy type) is represented by differently formatted lines. This is a line graph rather than a bar graph because the variable on the x-axis is quantitative with a small number of distinct levels. Line graphs are also appropriate when representing measurements made over a time interval (also referred to as time series information) on the x -axis.

Main Effects and Interactions

In factorial designs, there are two kinds of results that are of interest: main effects and interaction effects (which are also just called “interactions”). A main effect  is the statistical relationship between one independent variable and a dependent variable—averaging across the levels of the other independent variable. Thus there is one main effect to consider for each independent variable in the study. The top panel of Figure 8.3 shows a main effect of cell phone use because driving performance was better, on average, when participants were not using cell phones than when they were. The blue bars are, on average, higher than the red bars. It also shows a main effect of time of day because driving performance was better during the day than during the night—both when participants were using cell phones and when they were not. Main effects are independent of each other in the sense that whether or not there is a main effect of one independent variable says nothing about whether or not there is a main effect of the other. The bottom panel of Figure 8.3 , for example, shows a clear main effect of psychotherapy length. The longer the psychotherapy, the better it worked.

There is an  interaction  effect (or just “interaction”) when the effect of one independent variable depends on the level of another. Although this might seem complicated, you already have an intuitive understanding of interactions. It probably would not surprise you, for example, to hear that the effect of receiving psychotherapy is stronger among people who are highly motivated to change than among people who are not motivated to change. This is an interaction because the effect of one independent variable (whether or not one receives psychotherapy) depends on the level of another (motivation to change). Schnall and her colleagues also demonstrated an interaction because the effect of whether the room was clean or messy on participants’ moral judgments depended on whether the participants were low or high in private body consciousness. If they were high in private body consciousness, then those in the messy room made harsher judgments. If they were low in private body consciousness, then whether the room was clean or messy did not matter.

The effect of one independent variable can depend on the level of the other in several different ways. This is shown in Figure 8.4 . In the top panel, independent variable “B” has an effect at level 1 of independent variable “A” but no effect at level 2 of independent variable “A.” (This is much like the study of Schnall and her colleagues where there was an effect of disgust for those high in private body consciousness but not for those low in private body consciousness.) In the middle panel, independent variable “B” has a stronger effect at level 1 of independent variable “A” than at level 2. This is like the hypothetical driving example where there was a stronger effect of using a cell phone at night than during the day. In the bottom panel, independent variable “B” again has an effect at both levels of independent variable “A,” but the effects are in opposite directions. Figure 8.4 shows the strongest form of this kind of interaction, called a crossover interaction. One example of a crossover interaction comes from a study by Kathy Gilliland on the effect of caffeine on the verbal test scores of introverts and extraverts (Gilliland, 1980) [2] . Introverts perform better than extraverts when they have not ingested any caffeine. But extraverts perform better than introverts when they have ingested 4 mg of caffeine per kilogram of body weight.

Figure 8.5 shows examples of these same kinds of interactions when one of the independent variables is quantitative and the results are plotted in a line graph. Note that in a crossover interaction, the two lines literally “cross over” each other.

In many studies, the primary research question is about an interaction. The study by Brown and her colleagues was inspired by the idea that people with hypochondriasis are especially attentive to any negative health-related information. This led to the hypothesis that people high in hypochondriasis would recall negative health-related words more accurately than people low in hypochondriasis but recall non-health-related words about the same as people low in hypochondriasis. And of course this is exactly what happened in this study.

Key Takeaways

• Researchers often include multiple independent variables in their experiments. The most common approach is the factorial design, in which each level of one independent variable is combined with each level of the others to create all possible conditions.
• In a factorial design, the main effect of an independent variable is its overall effect averaged across all other independent variables. There is one main effect for each independent variable.
• There is an interaction between two independent variables when the effect of one depends on the level of the other. Some of the most interesting research questions and results in psychology are specifically about interactions.
• Practice: Return to the five article titles presented at the beginning of this section. For each one, identify the independent variables and the dependent variable.
• Practice: Create a factorial design table for an experiment on the effects of room temperature and noise level on performance on the MCAT. Be sure to indicate whether each independent variable will be manipulated between-subjects or within-subjects and explain why.
• No main effect of A; no main effect of B; no interaction
• Main effect of A; no main effect of B; no interaction
• No main effect of A; main effect of B; no interaction
• Main effect of A; main effect of B; no interaction
• Main effect of A; main effect of B; interaction
• Main effect of A; no main effect of B; interaction
• No main effect of A; main effect of B; interaction
• No main effect of A; no main effect of B; interaction

Image Descriptions

Figure 8.5 image description: Three panels, each showing a different line graph pattern. In the top panel, one line remains constant while the other goes up. In the middle panel, both lines go up but at different rates. In the bottom panel, one line goes down and the other goes up so they cross. [Return to Figure 8.5]

• Brown, H. D., Kosslyn, S. M., Delamater, B., Fama, A., & Barsky, A. J. (1999). Perceptual and memory biases for health-related information in hypochondriacal individuals. Journal of Psychosomatic Research, 47 , 67–78. ↵
• Gilliland, K. (1980). The interactive effect of introversion-extroversion with caffeine induced arousal on verbal performance. Journal of Research in Personality, 14 , 482–492. ↵

An approach to including multiple independent variables in an experiment where each level of one independent variable is combined with each level of the others to produce all possible combinations.

A table showing each condition produced by the combinations of variables.

All of the independent variables are manipulated between subjects.

When one independent variable is manipulated between subjects and another is manipulated within subjects.

In a factorial design, the researcher measures an independent variable but does not manipulate it.

In factorial design, the statistical relationship between one independent variable and a dependent variable--averaging across the levels of the other independent variable.

When the effect of one independent variable depends on the level of another.

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How to Write a Great Hypothesis

Hypothesis Definition, Format, Examples, and Tips

Kendra Cherry, MS, is a psychosocial rehabilitation specialist, psychology educator, and author of the "Everything Psychology Book."

Amy Morin, LCSW, is a psychotherapist and international bestselling author. Her books, including "13 Things Mentally Strong People Don't Do," have been translated into more than 40 languages. Her TEDx talk,  "The Secret of Becoming Mentally Strong," is one of the most viewed talks of all time.

Verywell / Alex Dos Diaz

• The Scientific Method

Hypothesis Format

Falsifiability of a hypothesis.

• Operationalization

Hypothesis Types

Hypotheses examples.

• Collecting Data

A hypothesis is a tentative statement about the relationship between two or more variables. It is a specific, testable prediction about what you expect to happen in a study. It is a preliminary answer to your question that helps guide the research process.

Consider a study designed to examine the relationship between sleep deprivation and test performance. The hypothesis might be: "This study is designed to assess the hypothesis that sleep-deprived people will perform worse on a test than individuals who are not sleep-deprived."

At a Glance

A hypothesis is crucial to scientific research because it offers a clear direction for what the researchers are looking to find. This allows them to design experiments to test their predictions and add to our scientific knowledge about the world. This article explores how a hypothesis is used in psychology research, how to write a good hypothesis, and the different types of hypotheses you might use.

The Hypothesis in the Scientific Method

In the scientific method , whether it involves research in psychology, biology, or some other area, a hypothesis represents what the researchers think will happen in an experiment. The scientific method involves the following steps:

• Forming a question
• Performing background research
• Creating a hypothesis
• Designing an experiment
• Collecting data
• Analyzing the results
• Drawing conclusions
• Communicating the results

The hypothesis is a prediction, but it involves more than a guess. Most of the time, the hypothesis begins with a question which is then explored through background research. At this point, researchers then begin to develop a testable hypothesis.

Unless you are creating an exploratory study, your hypothesis should always explain what you  expect  to happen.

In a study exploring the effects of a particular drug, the hypothesis might be that researchers expect the drug to have some type of effect on the symptoms of a specific illness. In psychology, the hypothesis might focus on how a certain aspect of the environment might influence a particular behavior.

Remember, a hypothesis does not have to be correct. While the hypothesis predicts what the researchers expect to see, the goal of the research is to determine whether this guess is right or wrong. When conducting an experiment, researchers might explore numerous factors to determine which ones might contribute to the ultimate outcome.

In many cases, researchers may find that the results of an experiment  do not  support the original hypothesis. When writing up these results, the researchers might suggest other options that should be explored in future studies.

In many cases, researchers might draw a hypothesis from a specific theory or build on previous research. For example, prior research has shown that stress can impact the immune system. So a researcher might hypothesize: "People with high-stress levels will be more likely to contract a common cold after being exposed to the virus than people who have low-stress levels."

In other instances, researchers might look at commonly held beliefs or folk wisdom. "Birds of a feather flock together" is one example of folk adage that a psychologist might try to investigate. The researcher might pose a specific hypothesis that "People tend to select romantic partners who are similar to them in interests and educational level."

Elements of a Good Hypothesis

So how do you write a good hypothesis? When trying to come up with a hypothesis for your research or experiments, ask yourself the following questions:

• Is your hypothesis based on your research on a topic?
• Can your hypothesis be tested?
• Does your hypothesis include independent and dependent variables?

Before you come up with a specific hypothesis, spend some time doing background research. Once you have completed a literature review, start thinking about potential questions you still have. Pay attention to the discussion section in the  journal articles you read . Many authors will suggest questions that still need to be explored.

How to Formulate a Good Hypothesis

To form a hypothesis, you should take these steps:

• Collect as many observations about a topic or problem as you can.
• Evaluate these observations and look for possible causes of the problem.
• Create a list of possible explanations that you might want to explore.
• After you have developed some possible hypotheses, think of ways that you could confirm or disprove each hypothesis through experimentation. This is known as falsifiability.

In the scientific method ,  falsifiability is an important part of any valid hypothesis. In order to test a claim scientifically, it must be possible that the claim could be proven false.

Students sometimes confuse the idea of falsifiability with the idea that it means that something is false, which is not the case. What falsifiability means is that  if  something was false, then it is possible to demonstrate that it is false.

One of the hallmarks of pseudoscience is that it makes claims that cannot be refuted or proven false.

The Importance of Operational Definitions

A variable is a factor or element that can be changed and manipulated in ways that are observable and measurable. However, the researcher must also define how the variable will be manipulated and measured in the study.

Operational definitions are specific definitions for all relevant factors in a study. This process helps make vague or ambiguous concepts detailed and measurable.

For example, a researcher might operationally define the variable " test anxiety " as the results of a self-report measure of anxiety experienced during an exam. A "study habits" variable might be defined by the amount of studying that actually occurs as measured by time.

These precise descriptions are important because many things can be measured in various ways. Clearly defining these variables and how they are measured helps ensure that other researchers can replicate your results.

Replicability

One of the basic principles of any type of scientific research is that the results must be replicable.

Replication means repeating an experiment in the same way to produce the same results. By clearly detailing the specifics of how the variables were measured and manipulated, other researchers can better understand the results and repeat the study if needed.

Some variables are more difficult than others to define. For example, how would you operationally define a variable such as aggression ? For obvious ethical reasons, researchers cannot create a situation in which a person behaves aggressively toward others.

To measure this variable, the researcher must devise a measurement that assesses aggressive behavior without harming others. The researcher might utilize a simulated task to measure aggressiveness in this situation.

Hypothesis Checklist

• Does your hypothesis focus on something that you can actually test?
• Does your hypothesis include both an independent and dependent variable?
• Can you manipulate the variables?
• Can your hypothesis be tested without violating ethical standards?

The hypothesis you use will depend on what you are investigating and hoping to find. Some of the main types of hypotheses that you might use include:

• Simple hypothesis : This type of hypothesis suggests there is a relationship between one independent variable and one dependent variable.
• Complex hypothesis : This type suggests a relationship between three or more variables, such as two independent and dependent variables.
• Null hypothesis : This hypothesis suggests no relationship exists between two or more variables.
• Alternative hypothesis : This hypothesis states the opposite of the null hypothesis.
• Statistical hypothesis : This hypothesis uses statistical analysis to evaluate a representative population sample and then generalizes the findings to the larger group.
• Logical hypothesis : This hypothesis assumes a relationship between variables without collecting data or evidence.

A hypothesis often follows a basic format of "If {this happens} then {this will happen}." One way to structure your hypothesis is to describe what will happen to the  dependent variable  if you change the  independent variable .

The basic format might be: "If {these changes are made to a certain independent variable}, then we will observe {a change in a specific dependent variable}."

A few examples of simple hypotheses:

• "Students who eat breakfast will perform better on a math exam than students who do not eat breakfast."
• "Students who experience test anxiety before an English exam will get lower scores than students who do not experience test anxiety."​
• "Motorists who talk on the phone while driving will be more likely to make errors on a driving course than those who do not talk on the phone."
• "Children who receive a new reading intervention will have higher reading scores than students who do not receive the intervention."

Examples of a complex hypothesis include:

• "People with high-sugar diets and sedentary activity levels are more likely to develop depression."
• "Younger people who are regularly exposed to green, outdoor areas have better subjective well-being than older adults who have limited exposure to green spaces."

Examples of a null hypothesis include:

• "There is no difference in anxiety levels between people who take St. John's wort supplements and those who do not."
• "There is no difference in scores on a memory recall task between children and adults."
• "There is no difference in aggression levels between children who play first-person shooter games and those who do not."

Examples of an alternative hypothesis:

• "People who take St. John's wort supplements will have less anxiety than those who do not."
• "Adults will perform better on a memory task than children."
• "Children who play first-person shooter games will show higher levels of aggression than children who do not." 

Collecting Data on Your Hypothesis

Once a researcher has formed a testable hypothesis, the next step is to select a research design and start collecting data. The research method depends largely on exactly what they are studying. There are two basic types of research methods: descriptive research and experimental research.

Descriptive Research Methods

Descriptive research such as  case studies ,  naturalistic observations , and surveys are often used when  conducting an experiment is difficult or impossible. These methods are best used to describe different aspects of a behavior or psychological phenomenon.

Once a researcher has collected data using descriptive methods, a  correlational study  can examine how the variables are related. This research method might be used to investigate a hypothesis that is difficult to test experimentally.

Experimental Research Methods

Experimental methods  are used to demonstrate causal relationships between variables. In an experiment, the researcher systematically manipulates a variable of interest (known as the independent variable) and measures the effect on another variable (known as the dependent variable).

Unlike correlational studies, which can only be used to determine if there is a relationship between two variables, experimental methods can be used to determine the actual nature of the relationship—whether changes in one variable actually  cause  another to change.

The hypothesis is a critical part of any scientific exploration. It represents what researchers expect to find in a study or experiment. In situations where the hypothesis is unsupported by the research, the research still has value. Such research helps us better understand how different aspects of the natural world relate to one another. It also helps us develop new hypotheses that can then be tested in the future.

Thompson WH, Skau S. On the scope of scientific hypotheses .  R Soc Open Sci . 2023;10(8):230607. doi:10.1098/rsos.230607

Taran S, Adhikari NKJ, Fan E. Falsifiability in medicine: what clinicians can learn from Karl Popper [published correction appears in Intensive Care Med. 2021 Jun 17;:].  Intensive Care Med . 2021;47(9):1054-1056. doi:10.1007/s00134-021-06432-z

Eyler AA. Research Methods for Public Health . 1st ed. Springer Publishing Company; 2020. doi:10.1891/9780826182067.0004

Nosek BA, Errington TM. What is replication ?  PLoS Biol . 2020;18(3):e3000691. doi:10.1371/journal.pbio.3000691

Aggarwal R, Ranganathan P. Study designs: Part 2 - Descriptive studies .  Perspect Clin Res . 2019;10(1):34-36. doi:10.4103/picr.PICR_154_18

Nevid J. Psychology: Concepts and Applications. Wadworth, 2013.

By Kendra Cherry, MSEd Kendra Cherry, MS, is a psychosocial rehabilitation specialist, psychology educator, and author of the "Everything Psychology Book."

Hypothesis Testing - Chi Squared Test

•   1  
• |   2  
• |   3  
• |   4  

Tests for Two or More Independent Samples, Discrete Outcome

Chi-squared tests in r.

Chi-Squared Table

All Modules

Here we extend that application of the chi-square test to the case with two or more independent comparison groups. Specifically, the outcome of interest is discrete with two or more responses and the responses can be ordered or unordered (i.e., the outcome can be dichotomous, ordinal or categorical). We now consider the situation where there are two or more independent comparison groups and the goal of the analysis is to compare the distribution of responses to the discrete outcome variable among several independent comparison groups.  

The test is called the χ 2 test of independence and the null hypothesis is that there is no difference in the distribution of responses to the outcome across comparison groups. This is often stated as follows: The outcome variable and the grouping variable (e.g., the comparison treatments or comparison groups) are independent (hence the name of the test). Independence here implies homogeneity in the distribution of the outcome among comparison groups.    

The null hypothesis in the χ 2 test of independence is often stated in words as: H 0 : The distribution of the outcome is independent of the groups. The alternative or research hypothesis is that there is a difference in the distribution of responses to the outcome variable among the comparison groups (i.e., that the distribution of responses "depends" on the group). In order to test the hypothesis, we measure the discrete outcome variable in each participant in each comparison group. The data of interest are the observed frequencies (or number of participants in each response category in each group). The formula for the test statistic for the χ 2 test of independence is given below.

Test Statistic for Testing H 0 : Distribution of outcome is independent of groups

and we find the critical value in a table of probabilities for the chi-square distribution with df=(r-1)*(c-1).

Here O = observed frequency, E=expected frequency in each of the response categories in each group, r = the number of rows in the two-way table and c = the number of columns in the two-way table.   r and c correspond to the number of comparison groups and the number of response options in the outcome (see below for more details). The observed frequencies are the sample data and the expected frequencies are computed as described below. The test statistic is appropriate for large samples, defined as expected frequencies of at least 5 in each of the response categories in each group.  

The data for the χ 2 test of independence are organized in a two-way table. The outcome and grouping variable are shown in the rows and columns of the table. The sample table below illustrates the data layout. The table entries (blank below) are the numbers of participants in each group responding to each response category of the outcome variable.

Table - Possible outcomes are are listed in the columns; The groups being compared are listed in rows.

In the table above, the grouping variable is shown in the rows of the table; r denotes the number of independent groups. The outcome variable is shown in the columns of the table; c denotes the number of response options in the outcome variable. Each combination of a row (group) and column (response) is called a cell of the table. The table has r*c cells and is sometimes called an r x c ("r by c") table. For example, if there are 4 groups and 5 categories in the outcome variable, the data are organized in a 4 X 5 table. The row and column totals are shown along the right-hand margin and the bottom of the table, respectively. The total sample size, N, can be computed by summing the row totals or the column totals. Similar to ANOVA, N does not refer to a population size here but rather to the total sample size in the analysis. The sample data can be organized into a table like the above. The numbers of participants within each group who select each response option are shown in the cells of the table and these are the observed frequencies used in the test statistic.

The test statistic for the χ 2 test of independence involves comparing observed (sample data) and expected frequencies in each cell of the table. The expected frequencies are computed assuming that the null hypothesis is true. The null hypothesis states that the two variables (the grouping variable and the outcome) are independent. The definition of independence is as follows:

 Two events, A and B, are independent if P(A|B) = P(A), or equivalently, if P(A and B) = P(A) P(B).

The second statement indicates that if two events, A and B, are independent then the probability of their intersection can be computed by multiplying the probability of each individual event. To conduct the χ 2 test of independence, we need to compute expected frequencies in each cell of the table. Expected frequencies are computed by assuming that the grouping variable and outcome are independent (i.e., under the null hypothesis). Thus, if the null hypothesis is true, using the definition of independence:

P(Group 1 and Response Option 1) = P(Group 1) P(Response Option 1).

 The above states that the probability that an individual is in Group 1 and their outcome is Response Option 1 is computed by multiplying the probability that person is in Group 1 by the probability that a person is in Response Option 1. To conduct the χ 2 test of independence, we need expected frequencies and not expected probabilities . To convert the above probability to a frequency, we multiply by N. Consider the following small example.

The data shown above are measured in a sample of size N=150. The frequencies in the cells of the table are the observed frequencies. If Group and Response are independent, then we can compute the probability that a person in the sample is in Group 1 and Response category 1 using:

P(Group 1 and Response 1) = P(Group 1) P(Response 1),

P(Group 1 and Response 1) = (25/150) (62/150) = 0.069.

Thus if Group and Response are independent we would expect 6.9% of the sample to be in the top left cell of the table (Group 1 and Response 1). The expected frequency is 150(0.069) = 10.4.   We could do the same for Group 2 and Response 1:

P(Group 2 and Response 1) = P(Group 2) P(Response 1),

P(Group 2 and Response 1) = (50/150) (62/150) = 0.138.

The expected frequency in Group 2 and Response 1 is 150(0.138) = 20.7.

Thus, the formula for determining the expected cell frequencies in the χ 2 test of independence is as follows:

Expected Cell Frequency = (Row Total * Column Total)/N.

The above computes the expected frequency in one step rather than computing the expected probability first and then converting to a frequency.  

In a prior example we evaluated data from a survey of university graduates which assessed, among other things, how frequently they exercised. The survey was completed by 470 graduates. In the prior example we used the χ 2 goodness-of-fit test to assess whether there was a shift in the distribution of responses to the exercise question following the implementation of a health promotion campaign on campus. We specifically considered one sample (all students) and compared the observed distribution to the distribution of responses the prior year (a historical control). Suppose we now wish to assess whether there is a relationship between exercise on campus and students' living arrangements. As part of the same survey, graduates were asked where they lived their senior year. The response options were dormitory, on-campus apartment, off-campus apartment, and at home (i.e., commuted to and from the university). The data are shown below.

Based on the data, is there a relationship between exercise and student's living arrangement? Do you think where a person lives affect their exercise status? Here we have four independent comparison groups (living arrangement) and a discrete (ordinal) outcome variable with three response options. We specifically want to test whether living arrangement and exercise are independent. We will run the test using the five-step approach.  

• Step 1. Set up hypotheses and determine level of significance.

H 0 : Living arrangement and exercise are independent

H 1 : H 0 is false.                α=0.05

The null and research hypotheses are written in words rather than in symbols. The research hypothesis is that the grouping variable (living arrangement) and the outcome variable (exercise) are dependent or related.   

• Step 2.  Select the appropriate test statistic.  

The formula for the test statistic is:

The condition for appropriate use of the above test statistic is that each expected frequency is at least 5. In Step 4 we will compute the expected frequencies and we will ensure that the condition is met.

• Step 3. Set up decision rule.  

The decision rule depends on the level of significance and the degrees of freedom, defined as df = (r-1)(c-1), where r and c are the numbers of rows and columns in the two-way data table.   The row variable is the living arrangement and there are 4 arrangements considered, thus r=4. The column variable is exercise and 3 responses are considered, thus c=3. For this test, df=(4-1)(3-1)=3(2)=6. Again, with χ 2 tests there are no upper, lower or two-tailed tests. If the null hypothesis is true, the observed and expected frequencies will be close in value and the χ 2 statistic will be close to zero. If the null hypothesis is false, then the χ 2 statistic will be large. The rejection region for the χ 2 test of independence is always in the upper (right-hand) tail of the distribution. For df=6 and a 5% level of significance, the appropriate critical value is 12.59 and the decision rule is as follows: Reject H 0 if c 2 > 12.59.

• Step 4. Compute the test statistic.  

We now compute the expected frequencies using the formula,

Expected Frequency = (Row Total * Column Total)/N.

The computations can be organized in a two-way table. The top number in each cell of the table is the observed frequency and the bottom number is the expected frequency.   The expected frequencies are shown in parentheses.

Notice that the expected frequencies are taken to one decimal place and that the sums of the observed frequencies are equal to the sums of the expected frequencies in each row and column of the table.  

Recall in Step 2 a condition for the appropriate use of the test statistic was that each expected frequency is at least 5. This is true for this sample (the smallest expected frequency is 9.6) and therefore it is appropriate to use the test statistic.

The test statistic is computed as follows:

• Step 5. Conclusion.  

We reject H 0 because 60.5 > 12.59. We have statistically significant evidence at a =0.05 to show that H 0 is false or that living arrangement and exercise are not independent (i.e., they are dependent or related), p < 0.005.  

Again, the χ 2 test of independence is used to test whether the distribution of the outcome variable is similar across the comparison groups. Here we rejected H 0 and concluded that the distribution of exercise is not independent of living arrangement, or that there is a relationship between living arrangement and exercise. The test provides an overall assessment of statistical significance. When the null hypothesis is rejected, it is important to review the sample data to understand the nature of the relationship. Consider again the sample data. 

Because there are different numbers of students in each living situation, it makes the comparisons of exercise patterns difficult on the basis of the frequencies alone. The following table displays the percentages of students in each exercise category by living arrangement. The percentages sum to 100% in each row of the table. For comparison purposes, percentages are also shown for the total sample along the bottom row of the table.

From the above, it is clear that higher percentages of students living in dormitories and in on-campus apartments reported regular exercise (31% and 23%) as compared to students living in off-campus apartments and at home (10% each).  

Test Yourself

( J Gastrointest Surgery, 2012, 16 275-281)', CAPTION, ' 

Question: What would be an appropriate statistical test to examine whether there is an association between Surgical Apgar Score and patient outcome? Using 14.13 as the value of the test statistic for these data, carry out the appropriate test at a 5% level of significance. Show all parts of your test.

In the module on hypothesis testing for means and proportions , we discussed hypothesis testing applications with a dichotomous outcome variable and two independent comparison groups. We presented a test using a test statistic Z to test for equality of independent proportions. The chi-square test of independence can also be used with a dichotomous outcome and the results are mathematically equivalent.  

In the prior module, we considered the following example. Here we show the equivalence to the chi-square test of independence.

A randomized trial is designed to evaluate the effectiveness of a newly developed pain reliever designed to reduce pain in patients following joint replacement surgery. The trial compares the new pain reliever to the pain reliever currently in use (called the standard of care). A total of 100 patients undergoing joint replacement surgery agreed to participate in the trial. Patients were randomly assigned to receive either the new pain reliever or the standard pain reliever following surgery and were blind to the treatment assignment. Before receiving the assigned treatment, patients were asked to rate their pain on a scale of 0-10 with higher scores indicative of more pain. Each patient was then given the assigned treatment and after 30 minutes was again asked to rate their pain on the same scale. The primary outcome was a reduction in pain of 3 or more scale points (defined by clinicians as a clinically meaningful reduction). The following data were observed in the trial.

We tested whether there was a significant difference in the proportions of patients reporting a meaningful reduction (i.e., a reduction of 3 or more scale points) using a Z statistic, as follows. 

• Step 1. Set up hypotheses and determine level of significance

H 0 : p 1 = p 2    

H 1 : p 1 ≠ p 2                             α=0.05

Here the new or experimental pain reliever is group 1 and the standard pain reliever is group 2.

• Step 2. Select the appropriate test statistic.  

We must first check that the sample size is adequate. Specifically, we need to ensure that we have at least 5 successes and 5 failures in each comparison group or that:

In this example, we have

Therefore, the sample size is adequate, so the following formula can be used:

Reject H 0 if Z < -1.960 or if Z > 1.960.

We now substitute the sample data into the formula for the test statistic identified in Step 2. We first compute the overall proportion of successes:

We now substitute to compute the test statistic.

• Step 5.  Conclusion.  

We now conduct the same test using the chi-square test of independence.  

H 0 : Treatment and outcome (meaningful reduction in pain) are independent

H 1 :   H 0 is false.         α=0.05

The formula for the test statistic is:  

For this test, df=(2-1)(2-1)=1. At a 5% level of significance, the appropriate critical value is 3.84 and the decision rule is as follows: Reject H0 if χ 2 > 3.84. (Note that 1.96 2 = 3.84, where 1.96 was the critical value used in the Z test for proportions shown above.)

We now compute the expected frequencies using:

The computations can be organized in a two-way table. The top number in each cell of the table is the observed frequency and the bottom number is the expected frequency. The expected frequencies are shown in parentheses.

A condition for the appropriate use of the test statistic was that each expected frequency is at least 5. This is true for this sample (the smallest expected frequency is 22.0) and therefore it is appropriate to use the test statistic.

(Note that (2.53) 2 = 6.4, where 2.53 was the value of the Z statistic in the test for proportions shown above.)

The video below by Mike Marin demonstrates how to perform chi-squared tests in the R programming language.

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Content ©2016. All Rights Reserved. Date last modified: September 1, 2016. Wayne W. LaMorte, MD, PhD, MPH

Research Variables 101

Independent variables, dependent variables, control variables and more

By: Derek Jansen (MBA) | Expert Reviewed By: Kerryn Warren (PhD) | January 2023

If you’re new to the world of research, especially scientific research, you’re bound to run into the concept of variables , sooner or later. If you’re feeling a little confused, don’t worry – you’re not the only one! Independent variables, dependent variables, confounding variables – it’s a lot of jargon. In this post, we’ll unpack the terminology surrounding research variables using straightforward language and loads of examples .

Overview: Variables In Research

What (exactly) is a variable.

The simplest way to understand a variable is as any characteristic or attribute that can experience change or vary over time or context – hence the name “variable”. For example, the dosage of a particular medicine could be classified as a variable, as the amount can vary (i.e., a higher dose or a lower dose). Similarly, gender, age or ethnicity could be considered demographic variables, because each person varies in these respects.

Within research, especially scientific research, variables form the foundation of studies, as researchers are often interested in how one variable impacts another, and the relationships between different variables. For example:

• How someone’s age impacts their sleep quality
• How different teaching methods impact learning outcomes
• How diet impacts weight (gain or loss)

As you can see, variables are often used to explain relationships between different elements and phenomena. In scientific studies, especially experimental studies, the objective is often to understand the causal relationships between variables. In other words, the role of cause and effect between variables. This is achieved by manipulating certain variables while controlling others – and then observing the outcome. But, we’ll get into that a little later…

The “Big 3” Variables

Variables can be a little intimidating for new researchers because there are a wide variety of variables, and oftentimes, there are multiple labels for the same thing. To lay a firm foundation, we’ll first look at the three main types of variables, namely:

• Independent variables (IV)
• Dependant variables (DV)
• Control variables

What is an independent variable?

Simply put, the independent variable is the “ cause ” in the relationship between two (or more) variables. In other words, when the independent variable changes, it has an impact on another variable.

For example:

• Increasing the dosage of a medication (Variable A) could result in better (or worse) health outcomes for a patient (Variable B)
• Changing a teaching method (Variable A) could impact the test scores that students earn in a standardised test (Variable B)
• Varying one’s diet (Variable A) could result in weight loss or gain (Variable B).

It’s useful to know that independent variables can go by a few different names, including, explanatory variables (because they explain an event or outcome) and predictor variables (because they predict the value of another variable). Terminology aside though, the most important takeaway is that independent variables are assumed to be the “cause” in any cause-effect relationship. As you can imagine, these types of variables are of major interest to researchers, as many studies seek to understand the causal factors behind a phenomenon.

Need a helping hand?

What is a dependent variable?

While the independent variable is the “ cause ”, the dependent variable is the “ effect ” – or rather, the affected variable . In other words, the dependent variable is the variable that is assumed to change as a result of a change in the independent variable.

Keeping with the previous example, let’s look at some dependent variables in action:

• Health outcomes (DV) could be impacted by dosage changes of a medication (IV)
• Students’ scores (DV) could be impacted by teaching methods (IV)
• Weight gain or loss (DV) could be impacted by diet (IV)

In scientific studies, researchers will typically pay very close attention to the dependent variable (or variables), carefully measuring any changes in response to hypothesised independent variables. This can be tricky in practice, as it’s not always easy to reliably measure specific phenomena or outcomes – or to be certain that the actual cause of the change is in fact the independent variable.

As the adage goes, correlation is not causation . In other words, just because two variables have a relationship doesn’t mean that it’s a causal relationship – they may just happen to vary together. For example, you could find a correlation between the number of people who own a certain brand of car and the number of people who have a certain type of job. Just because the number of people who own that brand of car and the number of people who have that type of job is correlated, it doesn’t mean that owning that brand of car causes someone to have that type of job or vice versa. The correlation could, for example, be caused by another factor such as income level or age group, which would affect both car ownership and job type.

To confidently establish a causal relationship between an independent variable and a dependent variable (i.e., X causes Y), you’ll typically need an experimental design , where you have complete control over the environmen t and the variables of interest. But even so, this doesn’t always translate into the “real world”. Simply put, what happens in the lab sometimes stays in the lab!

As an alternative to pure experimental research, correlational or “ quasi-experimental ” research (where the researcher cannot manipulate or change variables) can be done on a much larger scale more easily, allowing one to understand specific relationships in the real world. These types of studies also assume some causality between independent and dependent variables, but it’s not always clear. So, if you go this route, you need to be cautious in terms of how you describe the impact and causality between variables and be sure to acknowledge any limitations in your own research.

What is a control variable?

In an experimental design, a control variable (or controlled variable) is a variable that is intentionally held constant to ensure it doesn’t have an influence on any other variables. As a result, this variable remains unchanged throughout the course of the study. In other words, it’s a variable that’s not allowed to vary – tough life 🙂

As we mentioned earlier, one of the major challenges in identifying and measuring causal relationships is that it’s difficult to isolate the impact of variables other than the independent variable. Simply put, there’s always a risk that there are factors beyond the ones you’re specifically looking at that might be impacting the results of your study. So, to minimise the risk of this, researchers will attempt (as best possible) to hold other variables constant . These factors are then considered control variables.

Some examples of variables that you may need to control include:

• Temperature
• Time of day
• Noise or distractions

Which specific variables need to be controlled for will vary tremendously depending on the research project at hand, so there’s no generic list of control variables to consult. As a researcher, you’ll need to think carefully about all the factors that could vary within your research context and then consider how you’ll go about controlling them. A good starting point is to look at previous studies similar to yours and pay close attention to which variables they controlled for.

Of course, you won’t always be able to control every possible variable, and so, in many cases, you’ll just have to acknowledge their potential impact and account for them in the conclusions you draw. Every study has its limitations , so don’t get fixated or discouraged by troublesome variables. Nevertheless, always think carefully about the factors beyond what you’re focusing on – don’t make assumptions!

Other types of variables

As we mentioned, independent, dependent and control variables are the most common variables you’ll come across in your research, but they’re certainly not the only ones you need to be aware of. Next, we’ll look at a few “secondary” variables that you need to keep in mind as you design your research.

• Moderating variables
• Mediating variables
• Confounding variables
• Latent variables

Let’s jump into it…

What is a moderating variable?

A moderating variable is a variable that influences the strength or direction of the relationship between an independent variable and a dependent variable. In other words, moderating variables affect how much (or how little) the IV affects the DV, or whether the IV has a positive or negative relationship with the DV (i.e., moves in the same or opposite direction).

For example, in a study about the effects of sleep deprivation on academic performance, gender could be used as a moderating variable to see if there are any differences in how men and women respond to a lack of sleep. In such a case, one may find that gender has an influence on how much students’ scores suffer when they’re deprived of sleep.

It’s important to note that while moderators can have an influence on outcomes , they don’t necessarily cause them ; rather they modify or “moderate” existing relationships between other variables. This means that it’s possible for two different groups with similar characteristics, but different levels of moderation, to experience very different results from the same experiment or study design.

What is a mediating variable?

Mediating variables are often used to explain the relationship between the independent and dependent variable (s). For example, if you were researching the effects of age on job satisfaction, then education level could be considered a mediating variable, as it may explain why older people have higher job satisfaction than younger people – they may have more experience or better qualifications, which lead to greater job satisfaction.

Mediating variables also help researchers understand how different factors interact with each other to influence outcomes. For instance, if you wanted to study the effect of stress on academic performance, then coping strategies might act as a mediating factor by influencing both stress levels and academic performance simultaneously. For example, students who use effective coping strategies might be less stressed but also perform better academically due to their improved mental state.

In addition, mediating variables can provide insight into causal relationships between two variables by helping researchers determine whether changes in one factor directly cause changes in another – or whether there is an indirect relationship between them mediated by some third factor(s). For instance, if you wanted to investigate the impact of parental involvement on student achievement, you would need to consider family dynamics as a potential mediator, since it could influence both parental involvement and student achievement simultaneously.

What is a confounding variable?

A confounding variable (also known as a third variable or lurking variable ) is an extraneous factor that can influence the relationship between two variables being studied. Specifically, for a variable to be considered a confounding variable, it needs to meet two criteria:

• It must be correlated with the independent variable (this can be causal or not)
• It must have a causal impact on the dependent variable (i.e., influence the DV)

Some common examples of confounding variables include demographic factors such as gender, ethnicity, socioeconomic status, age, education level, and health status. In addition to these, there are also environmental factors to consider. For example, air pollution could confound the impact of the variables of interest in a study investigating health outcomes.

Naturally, it’s important to identify as many confounding variables as possible when conducting your research, as they can heavily distort the results and lead you to draw incorrect conclusions . So, always think carefully about what factors may have a confounding effect on your variables of interest and try to manage these as best you can.

What is a latent variable?

Latent variables are unobservable factors that can influence the behaviour of individuals and explain certain outcomes within a study. They’re also known as hidden or underlying variables , and what makes them rather tricky is that they can’t be directly observed or measured . Instead, latent variables must be inferred from other observable data points such as responses to surveys or experiments.

For example, in a study of mental health, the variable “resilience” could be considered a latent variable. It can’t be directly measured , but it can be inferred from measures of mental health symptoms, stress, and coping mechanisms. The same applies to a lot of concepts we encounter every day – for example:

• Emotional intelligence
• Quality of life
• Business confidence
• Ease of use

One way in which we overcome the challenge of measuring the immeasurable is latent variable models (LVMs). An LVM is a type of statistical model that describes a relationship between observed variables and one or more unobserved (latent) variables. These models allow researchers to uncover patterns in their data which may not have been visible before, thanks to their complexity and interrelatedness with other variables. Those patterns can then inform hypotheses about cause-and-effect relationships among those same variables which were previously unknown prior to running the LVM. Powerful stuff, we say!

Let’s recap

In the world of scientific research, there’s no shortage of variable types, some of which have multiple names and some of which overlap with each other. In this post, we’ve covered some of the popular ones, but remember that this is not an exhaustive list .

To recap, we’ve explored:

• Independent variables (the “cause”)
• Dependent variables (the “effect”)
• Control variables (the variable that’s not allowed to vary)

If you’re still feeling a bit lost and need a helping hand with your research project, check out our 1-on-1 coaching service , where we guide you through each step of the research journey. Also, be sure to check out our free dissertation writing course and our collection of free, fully-editable chapter templates .

Psst... there’s more!

This post was based on one of our popular Research Bootcamps . If you're working on a research project, you'll definitely want to check this out ...

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Examples of Independent and Dependent Variables

What Are Independent and Dependent Variables?

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• Ph.D., Biomedical Sciences, University of Tennessee at Knoxville
• B.A., Physics and Mathematics, Hastings College

Both the independent variable and dependent variable are examined in an experiment using the scientific method , so it's important to know what they are and how to use them.

In a scientific experiment, you'll ultimately be changing or controlling the independent variable and measuring the effect on the dependent variable. This distinction is critical in evaluating and proving hypotheses.

Below you'll find more about these two types of variables, along with examples of each in sample science experiments, and an explanation of how to graph them to help visualize your data.

What Is an Independent Variable?

An independent variable is the condition that you change in an experiment. In other words, it is the variable you control. It is called independent because its value does not depend on and is not affected by the state of any other variable in the experiment. Sometimes you may hear this variable called the "controlled variable" because it is the one that is changed. Do not confuse it with a control variable , which is a variable that is purposely held constant so that it can't affect the outcome of the experiment.

• What Is a Dependent Variable?

The dependent variable is the condition that you measure in an experiment. You are assessing how it responds to a change in the independent variable, so you can think of it as depending on the independent variable. Sometimes the dependent variable is called the "responding variable."

Independent and Dependent Variable Examples

• In a study to determine whether the amount of time a student sleeps affects test scores, the independent variable is the amount of time spent sleeping while the dependent variable is the test score.
• You want to compare brands of paper towels to see which holds the most liquid. The independent variable in your experiment would be the brand of paper towels. The dependent variable would be the amount of liquid absorbed by the paper towel.
• In an experiment to determine how far people can see into the infrared part of the spectrum, the wavelength of light is the independent variable and whether the light is observed (the response) is the dependent variable.
• If you want to know whether caffeine affects your appetite, the presence or absence of a given amount of caffeine would be the independent variable. How hungry you are would be the dependent variable.
• You want to determine whether a chemical is essential for rat nutrition, so you design an experiment. The presence or absence of the chemical is the independent variable. The health of the rat (whether it lives and can reproduce) is the dependent variable. If you determine the substance is necessary for proper nutrition, a follow-up experiment might determine how much of the chemical is needed. Here, the amount of the chemical would be the independent variable, and the rat's health would be the dependent variable.

How Do You Tell Independent and Dependent Variables Apart?

If you are having a hard time identifying which variable is the independent variable and which is the dependent variable, remember the dependent variable is the one affected by a change in the independent variable. If you write out the variables in a sentence that shows cause and effect, the independent variable causes the effect on the dependent variable. If you have the variables in the wrong order, the sentence won't make sense.

Independent variable causes an effect on the dependent variable.

Example : How long you sleep (independent variable) affects your test score (dependent variable).

This makes sense, but:

Example : Your test score affects how long you sleep.

This doesn't really make sense (unless you can't sleep because you are worried you failed a test, but that would be a different experiment).

How to Plot Variables on a Graph

There is a standard method for graphing independent and dependent variables. The x-axis is the independent variable, while the y-axis is the dependent variable. You can use the DRY MIX acronym to help remember how to graph variables:

D  = dependent variable R  = responding variable Y  = graph on the vertical or y-axis

M  = manipulated variable I  = independent variable X  = graph on the horizontal or x-axis

Test your understanding with the scientific method quiz .

Key Takeaways

• In scientific experiments, the independent variable is manipulated while the dependent variable is measured.
• The independent variable, controlled by the experimenter, influences the dependent variable, which responds to changes. This dynamic forms the basis of cause-and-effect relationships.
• Graphing independent and dependent variables follows a standard method in which the independent variable is plotted on the x-axis and the dependent variable on the y-axis.
• Difference Between Independent and Dependent Variables
• Dependent Variable Definition and Examples
• Scientific Variable
• Independent Variable Definition and Examples
• DRY MIX Experiment Variables Acronym
• What Is a Variable in Science?
• What Is an Experiment? Definition and Design
• Six Steps of the Scientific Method
• The Significance of Negative Slope
• The Differences Between Explanatory and Response Variables
• Scientific Method Flow Chart
• What Is a Hypothesis? (Science)
• The Role of a Controlled Variable in an Experiment
• Scientific Method Vocabulary Terms
• How To Design a Science Fair Experiment

Statistics Made Easy

Two Sample t-test: Definition, Formula, and Example

A two sample t-test is used to determine whether or not two population means are equal.

This tutorial explains the following:

• The motivation for performing a two sample t-test.
• The formula to perform a two sample t-test.
• The assumptions that should be met to perform a two sample t-test.
• An example of how to perform a two sample t-test.

Two Sample t-test: Motivation

Suppose we want to know whether or not the mean weight between two different species of turtles is equal. Since there are thousands of turtles in each population, it would be too time-consuming and costly to go around and weigh each individual turtle.

Instead, we might take a simple random sample of 15 turtles from each population and use the mean weight in each sample to determine if the mean weight is equal between the two populations:

However, it’s virtually guaranteed that the mean weight between the two samples will be at least a little different. The question is whether or not this difference is statistically significant . Fortunately, a two sample t-test allows us to answer this question.

Two Sample t-test: Formula

A two-sample t-test always uses the following null hypothesis:

• H 0 : μ 1  = μ 2 (the two population means are equal)

The alternative hypothesis can be either two-tailed, left-tailed, or right-tailed:

• H 1 (two-tailed): μ 1  ≠ μ 2 (the two population means are not equal)
• H 1 (left-tailed): μ 1  < μ 2  (population 1 mean is less than population 2 mean)
• H 1 (right-tailed):  μ 1 > μ 2  (population 1 mean is greater than population 2 mean)

We use the following formula to calculate the test statistic t:

Test statistic:  ( x 1  –  x 2 )  /  s p (√ 1/n 1  + 1/n 2 )

where  x 1  and  x 2 are the sample means, n 1 and n 2  are the sample sizes, and where s p is calculated as:

s p = √  (n 1 -1)s 1 2  +  (n 2 -1)s 2 2  /  (n 1 +n 2 -2)

where s 1 2  and s 2 2  are the sample variances.

If the p-value that corresponds to the test statistic t with (n 1 +n 2 -1) degrees of freedom is less than your chosen significance level (common choices are 0.10, 0.05, and 0.01) then you can reject the null hypothesis.

Two Sample t-test: Assumptions

For the results of a two sample t-test to be valid, the following assumptions should be met:

• The observations in one sample should be independent of the observations in the other sample.
• The data should be approximately normally distributed.
• The two samples should have approximately the same variance. If this assumption is not met, you should instead perform Welch’s t-test .
• The data in both samples was obtained using a random sampling method .

Two Sample t-test : Example

Suppose we want to know whether or not the mean weight between two different species of turtles is equal. To test this, will perform a two sample t-test at significance level α = 0.05 using the following steps:

Step 1: Gather the sample data.

Suppose we collect a random sample of turtles from each population with the following information:

• Sample size n 1 = 40
• Sample mean weight  x 1  = 300
• Sample standard deviation s 1 = 18.5
• Sample size n 2 = 38
• Sample mean weight  x 2  = 305
• Sample standard deviation s 2 = 16.7

Step 2: Define the hypotheses.

We will perform the two sample t-test with the following hypotheses:

• H 0 :  μ 1  = μ 2 (the two population means are equal)
• H 1 :  μ 1  ≠ μ 2 (the two population means are not equal)

Step 3: Calculate the test statistic  t .

First, we will calculate the pooled standard deviation s p :

s p = √  (n 1 -1)s 1 2  +  (n 2 -1)s 2 2  /  (n 1 +n 2 -2)  = √  (40-1)18.5 2  +  (38-1)16.7 2  /  (40+38-2)  = 17.647

Next, we will calculate the test statistic  t :

t = ( x 1  –  x 2 )  /  s p (√ 1/n 1  + 1/n 2 ) =  (300-305) / 17.647(√ 1/40 + 1/38 ) =  -1.2508

Step 4: Calculate the p-value of the test statistic  t .

According to the T Score to P Value Calculator , the p-value associated with t = -1.2508 and degrees of freedom = n 1 +n 2 -2 = 40+38-2 = 76 is  0.21484 .

Step 5: Draw a conclusion.

Since this p-value is not less than our significance level α = 0.05, we fail to reject the null hypothesis. We do not have sufficient evidence to say that the mean weight of turtles between these two populations is different.

Note:  You can also perform this entire two sample t-test by simply using the Two Sample t-test Calculator .

Additional Resources

The following tutorials explain how to perform a two-sample t-test using different statistical programs:

How to Perform a Two Sample t-test in Excel How to Perform a Two Sample t-test in SPSS How to Perform a Two Sample t-test in Stata How to Perform a Two Sample t-test in R How to Perform a Two Sample t-test in Python How to Perform a Two Sample t-test on a TI-84 Calculator

Featured Posts

Hey there. My name is Zach Bobbitt. I have a Masters of Science degree in Applied Statistics and I’ve worked on machine learning algorithms for professional businesses in both healthcare and retail. I’m passionate about statistics, machine learning, and data visualization and I created Statology to be a resource for both students and teachers alike.  My goal with this site is to help you learn statistics through using simple terms, plenty of real-world examples, and helpful illustrations.

2 Replies to “Two Sample t-test: Definition, Formula, and Example”

I like the detailed information and simplified in the way I can understand and relate easily. Thank you

It seems a couple of parenthesis is missed at the pooled standard deviation formula. Under square root you have (n1-1)s12 + (n2-1)s22 / (n1+n2-2) but it should be [(n1-1)s12 + (n2-1)s22] / (n1+n2-2) I used square bracket

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Independent and Dependent Variables Examples

The independent and dependent variables are key to any scientific experiment, but how do you tell them apart? Here are the definitions of independent and dependent variables, examples of each type, and tips for telling them apart and graphing them.

Independent Variable

The independent variable is the factor the researcher changes or controls in an experiment. It is called independent because it does not depend on any other variable. The independent variable may be called the “controlled variable” because it is the one that is changed or controlled. This is different from the “ control variable ,” which is variable that is held constant so it won’t influence the outcome of the experiment.

Dependent Variable

The dependent variable is the factor that changes in response to the independent variable. It is the variable that you measure in an experiment. The dependent variable may be called the “responding variable.”

Examples of Independent and Dependent Variables

Here are several examples of independent and dependent variables in experiments:

• In a study to determine whether how long a student sleeps affects test scores, the independent variable is the length of time spent sleeping while the dependent variable is the test score.
• You want to know which brand of fertilizer is best for your plants. The brand of fertilizer is the independent variable. The health of the plants (height, amount and size of flowers and fruit, color) is the dependent variable.
• You want to compare brands of paper towels, to see which holds the most liquid. The independent variable is the brand of paper towel. The dependent variable is the volume of liquid absorbed by the paper towel.
• You suspect the amount of television a person watches is related to their age. Age is the independent variable. How many minutes or hours of television a person watches is the dependent variable.
• You think rising sea temperatures might affect the amount of algae in the water. The water temperature is the independent variable. The mass of algae is the dependent variable.
• In an experiment to determine how far people can see into the infrared part of the spectrum, the wavelength of light is the independent variable and whether the light is observed is the dependent variable.
• If you want to know whether caffeine affects your appetite, the presence/absence or amount of caffeine is the independent variable. Appetite is the dependent variable.
• You want to know which brand of microwave popcorn pops the best. The brand of popcorn is the independent variable. The number of popped kernels is the dependent variable. Of course, you could also measure the number of unpopped kernels instead.
• You want to determine whether a chemical is essential for rat nutrition, so you design an experiment. The presence/absence of the chemical is the independent variable. The health of the rat (whether it lives and reproduces) is the dependent variable. A follow-up experiment might determine how much of the chemical is needed. Here, the amount of chemical is the independent variable and the rat health is the dependent variable.

How to Tell the Independent and Dependent Variable Apart

If you’re having trouble identifying the independent and dependent variable, here are a few ways to tell them apart. First, remember the dependent variable depends on the independent variable. It helps to write out the variables as an if-then or cause-and-effect sentence that shows the independent variable causes an effect on the dependent variable. If you mix up the variables, the sentence won’t make sense. Example : The amount of eat (independent variable) affects how much you weigh (dependent variable).

This makes sense, but if you write the sentence the other way, you can tell it’s incorrect: Example : How much you weigh affects how much you eat. (Well, it could make sense, but you can see it’s an entirely different experiment.) If-then statements also work: Example : If you change the color of light (independent variable), then it affects plant growth (dependent variable). Switching the variables makes no sense: Example : If plant growth rate changes, then it affects the color of light. Sometimes you don’t control either variable, like when you gather data to see if there is a relationship between two factors. This can make identifying the variables a bit trickier, but establishing a logical cause and effect relationship helps: Example : If you increase age (independent variable), then average salary increases (dependent variable). If you switch them, the statement doesn’t make sense: Example : If you increase salary, then age increases.

How to Graph Independent and Dependent Variables

Plot or graph independent and dependent variables using the standard method. The independent variable is the x-axis, while the dependent variable is the y-axis. Remember the acronym DRY MIX to keep the variables straight: D = Dependent variable R = Responding variable/ Y = Graph on the y-axis or vertical axis M = Manipulated variable I = Independent variable X = Graph on the x-axis or horizontal axis

• Babbie, Earl R. (2009). The Practice of Social Research (12th ed.) Wadsworth Publishing. ISBN 0-495-59841-0.
• di Francia, G. Toraldo (1981). The Investigation of the Physical World . Cambridge University Press. ISBN 978-0-521-29925-1.
• Gauch, Hugh G. Jr. (2003). Scientific Method in Practice . Cambridge University Press. ISBN 978-0-521-01708-4.
• Popper, Karl R. (2003). Conjectures and Refutations: The Growth of Scientific Knowledge . Routledge. ISBN 0-415-28594-1.

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