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Biology library

Course: biology library   >   unit 1, the scientific method.

  • Controlled experiments
  • The scientific method and experimental design

Introduction

  • Make an observation.
  • Ask a question.
  • Form a hypothesis , or testable explanation.
  • Make a prediction based on the hypothesis.
  • Test the prediction.
  • Iterate: use the results to make new hypotheses or predictions.

Scientific method example: Failure to toast

1. make an observation..

  • Observation: the toaster won't toast.

2. Ask a question.

  • Question: Why won't my toaster toast?

3. Propose a hypothesis.

  • Hypothesis: Maybe the outlet is broken.

4. Make predictions.

  • Prediction: If I plug the toaster into a different outlet, then it will toast the bread.

5. Test the predictions.

  • Test of prediction: Plug the toaster into a different outlet and try again.
  • If the toaster does toast, then the hypothesis is supported—likely correct.
  • If the toaster doesn't toast, then the hypothesis is not supported—likely wrong.

Logical possibility

Practical possibility, building a body of evidence, 6. iterate..

  • Iteration time!
  • If the hypothesis was supported, we might do additional tests to confirm it, or revise it to be more specific. For instance, we might investigate why the outlet is broken.
  • If the hypothesis was not supported, we would come up with a new hypothesis. For instance, the next hypothesis might be that there's a broken wire in the toaster.

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Incredible Answer

Scientific Hypothesis, Model, Theory, and Law

Understanding the Difference Between Basic Scientific Terms

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  • Ph.D., Biomedical Sciences, University of Tennessee at Knoxville
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Words have precise meanings in science. For example, "theory," "law," and "hypothesis" don't all mean the same thing. Outside of science, you might say something is "just a theory," meaning it's a supposition that may or may not be true. In science, however, a theory is an explanation that generally is accepted to be true. Here's a closer look at these important, commonly misused terms.

A hypothesis is an educated guess, based on observation. It's a prediction of cause and effect. Usually, a hypothesis can be supported or refuted through experimentation or more observation. A hypothesis can be disproven but not proven to be true.

Example: If you see no difference in the cleaning ability of various laundry detergents, you might hypothesize that cleaning effectiveness is not affected by which detergent you use. This hypothesis can be disproven if you observe a stain is removed by one detergent and not another. On the other hand, you cannot prove the hypothesis. Even if you never see a difference in the cleanliness of your clothes after trying 1,000 detergents, there might be one more you haven't tried that could be different.

Scientists often construct models to help explain complex concepts. These can be physical models like a model volcano or atom  or conceptual models like predictive weather algorithms. A model doesn't contain all the details of the real deal, but it should include observations known to be valid.

Example: The  Bohr model shows electrons orbiting the atomic nucleus, much the same way as the way planets revolve around the sun. In reality, the movement of electrons is complicated but the model makes it clear that protons and neutrons form a nucleus and electrons tend to move around outside the nucleus.

A scientific theory summarizes a hypothesis or group of hypotheses that have been supported with repeated testing. A theory is valid as long as there is no evidence to dispute it. Therefore, theories can be disproven. Basically, if evidence accumulates to support a hypothesis, then the hypothesis can become accepted as a good explanation of a phenomenon. One definition of a theory is to say that it's an accepted hypothesis.

Example: It is known that on June 30, 1908, in Tunguska, Siberia, there was an explosion equivalent to the detonation of about 15 million tons of TNT. Many hypotheses have been proposed for what caused the explosion. It was theorized that the explosion was caused by a natural extraterrestrial phenomenon , and was not caused by man. Is this theory a fact? No. The event is a recorded fact. Is this theory, generally accepted to be true, based on evidence to-date? Yes. Can this theory be shown to be false and be discarded? Yes.

A scientific law generalizes a body of observations. At the time it's made, no exceptions have been found to a law. Scientific laws explain things but they do not describe them. One way to tell a law and a theory apart is to ask if the description gives you the means to explain "why." The word "law" is used less and less in science, as many laws are only true under limited circumstances.

Example: Consider Newton's Law of Gravity . Newton could use this law to predict the behavior of a dropped object but he couldn't explain why it happened.

As you can see, there is no "proof" or absolute "truth" in science. The closest we get are facts, which are indisputable observations. Note, however, if you define proof as arriving at a logical conclusion, based on the evidence, then there is "proof" in science. Some work under the definition that to prove something implies it can never be wrong, which is different. If you're asked to define the terms hypothesis, theory, and law, keep in mind the definitions of proof and of these words can vary slightly depending on the scientific discipline. What's important is to realize they don't all mean the same thing and cannot be used interchangeably.

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  • What Is a Hypothesis? (Science)
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  • Processual Archaeology
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  • What Is the Difference Between Hard and Soft Science?
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  • Geological Thinking: Method of Multiple Working Hypotheses
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What is a scientific hypothesis?

It's the initial building block in the scientific method.

A girl looks at plants in a test tube for a science experiment. What's her scientific hypothesis?

Hypothesis basics

What makes a hypothesis testable.

  • Types of hypotheses
  • Hypothesis versus theory

Additional resources

Bibliography.

A scientific hypothesis is a tentative, testable explanation for a phenomenon in the natural world. It's the initial building block in the scientific method . Many describe it as an "educated guess" based on prior knowledge and observation. While this is true, a hypothesis is more informed than a guess. While an "educated guess" suggests a random prediction based on a person's expertise, developing a hypothesis requires active observation and background research. 

The basic idea of a hypothesis is that there is no predetermined outcome. For a solution to be termed a scientific hypothesis, it has to be an idea that can be supported or refuted through carefully crafted experimentation or observation. This concept, called falsifiability and testability, was advanced in the mid-20th century by Austrian-British philosopher Karl Popper in his famous book "The Logic of Scientific Discovery" (Routledge, 1959).

A key function of a hypothesis is to derive predictions about the results of future experiments and then perform those experiments to see whether they support the predictions.

A hypothesis is usually written in the form of an if-then statement, which gives a possibility (if) and explains what may happen because of the possibility (then). The statement could also include "may," according to California State University, Bakersfield .

Here are some examples of hypothesis statements:

  • If garlic repels fleas, then a dog that is given garlic every day will not get fleas.
  • If sugar causes cavities, then people who eat a lot of candy may be more prone to cavities.
  • If ultraviolet light can damage the eyes, then maybe this light can cause blindness.

A useful hypothesis should be testable and falsifiable. That means that it should be possible to prove it wrong. A theory that can't be proved wrong is nonscientific, according to Karl Popper's 1963 book " Conjectures and Refutations ."

An example of an untestable statement is, "Dogs are better than cats." That's because the definition of "better" is vague and subjective. However, an untestable statement can be reworded to make it testable. For example, the previous statement could be changed to this: "Owning a dog is associated with higher levels of physical fitness than owning a cat." With this statement, the researcher can take measures of physical fitness from dog and cat owners and compare the two.

Types of scientific hypotheses

Elementary-age students study alternative energy using homemade windmills during public school science class.

In an experiment, researchers generally state their hypotheses in two ways. The null hypothesis predicts that there will be no relationship between the variables tested, or no difference between the experimental groups. The alternative hypothesis predicts the opposite: that there will be a difference between the experimental groups. This is usually the hypothesis scientists are most interested in, according to the University of Miami .

For example, a null hypothesis might state, "There will be no difference in the rate of muscle growth between people who take a protein supplement and people who don't." The alternative hypothesis would state, "There will be a difference in the rate of muscle growth between people who take a protein supplement and people who don't."

If the results of the experiment show a relationship between the variables, then the null hypothesis has been rejected in favor of the alternative hypothesis, according to the book " Research Methods in Psychology " (​​BCcampus, 2015). 

There are other ways to describe an alternative hypothesis. The alternative hypothesis above does not specify a direction of the effect, only that there will be a difference between the two groups. That type of prediction is called a two-tailed hypothesis. If a hypothesis specifies a certain direction — for example, that people who take a protein supplement will gain more muscle than people who don't — it is called a one-tailed hypothesis, according to William M. K. Trochim , a professor of Policy Analysis and Management at Cornell University.

Sometimes, errors take place during an experiment. These errors can happen in one of two ways. A type I error is when the null hypothesis is rejected when it is true. This is also known as a false positive. A type II error occurs when the null hypothesis is not rejected when it is false. This is also known as a false negative, according to the University of California, Berkeley . 

A hypothesis can be rejected or modified, but it can never be proved correct 100% of the time. For example, a scientist can form a hypothesis stating that if a certain type of tomato has a gene for red pigment, that type of tomato will be red. During research, the scientist then finds that each tomato of this type is red. Though the findings confirm the hypothesis, there may be a tomato of that type somewhere in the world that isn't red. Thus, the hypothesis is true, but it may not be true 100% of the time.

Scientific theory vs. scientific hypothesis

The best hypotheses are simple. They deal with a relatively narrow set of phenomena. But theories are broader; they generally combine multiple hypotheses into a general explanation for a wide range of phenomena, according to the University of California, Berkeley . For example, a hypothesis might state, "If animals adapt to suit their environments, then birds that live on islands with lots of seeds to eat will have differently shaped beaks than birds that live on islands with lots of insects to eat." After testing many hypotheses like these, Charles Darwin formulated an overarching theory: the theory of evolution by natural selection.

"Theories are the ways that we make sense of what we observe in the natural world," Tanner said. "Theories are structures of ideas that explain and interpret facts." 

  • Read more about writing a hypothesis, from the American Medical Writers Association.
  • Find out why a hypothesis isn't always necessary in science, from The American Biology Teacher.
  • Learn about null and alternative hypotheses, from Prof. Essa on YouTube .

Encyclopedia Britannica. Scientific Hypothesis. Jan. 13, 2022. https://www.britannica.com/science/scientific-hypothesis

Karl Popper, "The Logic of Scientific Discovery," Routledge, 1959.

California State University, Bakersfield, "Formatting a testable hypothesis." https://www.csub.edu/~ddodenhoff/Bio100/Bio100sp04/formattingahypothesis.htm  

Karl Popper, "Conjectures and Refutations," Routledge, 1963.

Price, P., Jhangiani, R., & Chiang, I., "Research Methods of Psychology — 2nd Canadian Edition," BCcampus, 2015.‌

University of Miami, "The Scientific Method" http://www.bio.miami.edu/dana/161/evolution/161app1_scimethod.pdf  

William M.K. Trochim, "Research Methods Knowledge Base," https://conjointly.com/kb/hypotheses-explained/  

University of California, Berkeley, "Multiple Hypothesis Testing and False Discovery Rate" https://www.stat.berkeley.edu/~hhuang/STAT141/Lecture-FDR.pdf  

University of California, Berkeley, "Science at multiple levels" https://undsci.berkeley.edu/article/0_0_0/howscienceworks_19

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which statement is a valid scientific hypothesis

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How to Write a Great Hypothesis

Hypothesis Definition, Format, Examples, and Tips

Kendra Cherry, MS, is a psychosocial rehabilitation specialist, psychology educator, and author of the "Everything Psychology Book."

which statement is a valid scientific hypothesis

Amy Morin, LCSW, is a psychotherapist and international bestselling author. Her books, including "13 Things Mentally Strong People Don't Do," have been translated into more than 40 languages. Her TEDx talk,  "The Secret of Becoming Mentally Strong," is one of the most viewed talks of all time.

which statement is a valid scientific hypothesis

Verywell / Alex Dos Diaz

  • The Scientific Method

Hypothesis Format

Falsifiability of a hypothesis.

  • Operationalization

Hypothesis Types

Hypotheses examples.

  • Collecting Data

A hypothesis is a tentative statement about the relationship between two or more variables. It is a specific, testable prediction about what you expect to happen in a study. It is a preliminary answer to your question that helps guide the research process.

Consider a study designed to examine the relationship between sleep deprivation and test performance. The hypothesis might be: "This study is designed to assess the hypothesis that sleep-deprived people will perform worse on a test than individuals who are not sleep-deprived."

At a Glance

A hypothesis is crucial to scientific research because it offers a clear direction for what the researchers are looking to find. This allows them to design experiments to test their predictions and add to our scientific knowledge about the world. This article explores how a hypothesis is used in psychology research, how to write a good hypothesis, and the different types of hypotheses you might use.

The Hypothesis in the Scientific Method

In the scientific method , whether it involves research in psychology, biology, or some other area, a hypothesis represents what the researchers think will happen in an experiment. The scientific method involves the following steps:

  • Forming a question
  • Performing background research
  • Creating a hypothesis
  • Designing an experiment
  • Collecting data
  • Analyzing the results
  • Drawing conclusions
  • Communicating the results

The hypothesis is a prediction, but it involves more than a guess. Most of the time, the hypothesis begins with a question which is then explored through background research. At this point, researchers then begin to develop a testable hypothesis.

Unless you are creating an exploratory study, your hypothesis should always explain what you  expect  to happen.

In a study exploring the effects of a particular drug, the hypothesis might be that researchers expect the drug to have some type of effect on the symptoms of a specific illness. In psychology, the hypothesis might focus on how a certain aspect of the environment might influence a particular behavior.

Remember, a hypothesis does not have to be correct. While the hypothesis predicts what the researchers expect to see, the goal of the research is to determine whether this guess is right or wrong. When conducting an experiment, researchers might explore numerous factors to determine which ones might contribute to the ultimate outcome.

In many cases, researchers may find that the results of an experiment  do not  support the original hypothesis. When writing up these results, the researchers might suggest other options that should be explored in future studies.

In many cases, researchers might draw a hypothesis from a specific theory or build on previous research. For example, prior research has shown that stress can impact the immune system. So a researcher might hypothesize: "People with high-stress levels will be more likely to contract a common cold after being exposed to the virus than people who have low-stress levels."

In other instances, researchers might look at commonly held beliefs or folk wisdom. "Birds of a feather flock together" is one example of folk adage that a psychologist might try to investigate. The researcher might pose a specific hypothesis that "People tend to select romantic partners who are similar to them in interests and educational level."

Elements of a Good Hypothesis

So how do you write a good hypothesis? When trying to come up with a hypothesis for your research or experiments, ask yourself the following questions:

  • Is your hypothesis based on your research on a topic?
  • Can your hypothesis be tested?
  • Does your hypothesis include independent and dependent variables?

Before you come up with a specific hypothesis, spend some time doing background research. Once you have completed a literature review, start thinking about potential questions you still have. Pay attention to the discussion section in the  journal articles you read . Many authors will suggest questions that still need to be explored.

How to Formulate a Good Hypothesis

To form a hypothesis, you should take these steps:

  • Collect as many observations about a topic or problem as you can.
  • Evaluate these observations and look for possible causes of the problem.
  • Create a list of possible explanations that you might want to explore.
  • After you have developed some possible hypotheses, think of ways that you could confirm or disprove each hypothesis through experimentation. This is known as falsifiability.

In the scientific method ,  falsifiability is an important part of any valid hypothesis. In order to test a claim scientifically, it must be possible that the claim could be proven false.

Students sometimes confuse the idea of falsifiability with the idea that it means that something is false, which is not the case. What falsifiability means is that  if  something was false, then it is possible to demonstrate that it is false.

One of the hallmarks of pseudoscience is that it makes claims that cannot be refuted or proven false.

The Importance of Operational Definitions

A variable is a factor or element that can be changed and manipulated in ways that are observable and measurable. However, the researcher must also define how the variable will be manipulated and measured in the study.

Operational definitions are specific definitions for all relevant factors in a study. This process helps make vague or ambiguous concepts detailed and measurable.

For example, a researcher might operationally define the variable " test anxiety " as the results of a self-report measure of anxiety experienced during an exam. A "study habits" variable might be defined by the amount of studying that actually occurs as measured by time.

These precise descriptions are important because many things can be measured in various ways. Clearly defining these variables and how they are measured helps ensure that other researchers can replicate your results.

Replicability

One of the basic principles of any type of scientific research is that the results must be replicable.

Replication means repeating an experiment in the same way to produce the same results. By clearly detailing the specifics of how the variables were measured and manipulated, other researchers can better understand the results and repeat the study if needed.

Some variables are more difficult than others to define. For example, how would you operationally define a variable such as aggression ? For obvious ethical reasons, researchers cannot create a situation in which a person behaves aggressively toward others.

To measure this variable, the researcher must devise a measurement that assesses aggressive behavior without harming others. The researcher might utilize a simulated task to measure aggressiveness in this situation.

Hypothesis Checklist

  • Does your hypothesis focus on something that you can actually test?
  • Does your hypothesis include both an independent and dependent variable?
  • Can you manipulate the variables?
  • Can your hypothesis be tested without violating ethical standards?

The hypothesis you use will depend on what you are investigating and hoping to find. Some of the main types of hypotheses that you might use include:

  • Simple hypothesis : This type of hypothesis suggests there is a relationship between one independent variable and one dependent variable.
  • Complex hypothesis : This type suggests a relationship between three or more variables, such as two independent and dependent variables.
  • Null hypothesis : This hypothesis suggests no relationship exists between two or more variables.
  • Alternative hypothesis : This hypothesis states the opposite of the null hypothesis.
  • Statistical hypothesis : This hypothesis uses statistical analysis to evaluate a representative population sample and then generalizes the findings to the larger group.
  • Logical hypothesis : This hypothesis assumes a relationship between variables without collecting data or evidence.

A hypothesis often follows a basic format of "If {this happens} then {this will happen}." One way to structure your hypothesis is to describe what will happen to the  dependent variable  if you change the  independent variable .

The basic format might be: "If {these changes are made to a certain independent variable}, then we will observe {a change in a specific dependent variable}."

A few examples of simple hypotheses:

  • "Students who eat breakfast will perform better on a math exam than students who do not eat breakfast."
  • "Students who experience test anxiety before an English exam will get lower scores than students who do not experience test anxiety."​
  • "Motorists who talk on the phone while driving will be more likely to make errors on a driving course than those who do not talk on the phone."
  • "Children who receive a new reading intervention will have higher reading scores than students who do not receive the intervention."

Examples of a complex hypothesis include:

  • "People with high-sugar diets and sedentary activity levels are more likely to develop depression."
  • "Younger people who are regularly exposed to green, outdoor areas have better subjective well-being than older adults who have limited exposure to green spaces."

Examples of a null hypothesis include:

  • "There is no difference in anxiety levels between people who take St. John's wort supplements and those who do not."
  • "There is no difference in scores on a memory recall task between children and adults."
  • "There is no difference in aggression levels between children who play first-person shooter games and those who do not."

Examples of an alternative hypothesis:

  • "People who take St. John's wort supplements will have less anxiety than those who do not."
  • "Adults will perform better on a memory task than children."
  • "Children who play first-person shooter games will show higher levels of aggression than children who do not." 

Collecting Data on Your Hypothesis

Once a researcher has formed a testable hypothesis, the next step is to select a research design and start collecting data. The research method depends largely on exactly what they are studying. There are two basic types of research methods: descriptive research and experimental research.

Descriptive Research Methods

Descriptive research such as  case studies ,  naturalistic observations , and surveys are often used when  conducting an experiment is difficult or impossible. These methods are best used to describe different aspects of a behavior or psychological phenomenon.

Once a researcher has collected data using descriptive methods, a  correlational study  can examine how the variables are related. This research method might be used to investigate a hypothesis that is difficult to test experimentally.

Experimental Research Methods

Experimental methods  are used to demonstrate causal relationships between variables. In an experiment, the researcher systematically manipulates a variable of interest (known as the independent variable) and measures the effect on another variable (known as the dependent variable).

Unlike correlational studies, which can only be used to determine if there is a relationship between two variables, experimental methods can be used to determine the actual nature of the relationship—whether changes in one variable actually  cause  another to change.

The hypothesis is a critical part of any scientific exploration. It represents what researchers expect to find in a study or experiment. In situations where the hypothesis is unsupported by the research, the research still has value. Such research helps us better understand how different aspects of the natural world relate to one another. It also helps us develop new hypotheses that can then be tested in the future.

Thompson WH, Skau S. On the scope of scientific hypotheses .  R Soc Open Sci . 2023;10(8):230607. doi:10.1098/rsos.230607

Taran S, Adhikari NKJ, Fan E. Falsifiability in medicine: what clinicians can learn from Karl Popper [published correction appears in Intensive Care Med. 2021 Jun 17;:].  Intensive Care Med . 2021;47(9):1054-1056. doi:10.1007/s00134-021-06432-z

Eyler AA. Research Methods for Public Health . 1st ed. Springer Publishing Company; 2020. doi:10.1891/9780826182067.0004

Nosek BA, Errington TM. What is replication ?  PLoS Biol . 2020;18(3):e3000691. doi:10.1371/journal.pbio.3000691

Aggarwal R, Ranganathan P. Study designs: Part 2 - Descriptive studies .  Perspect Clin Res . 2019;10(1):34-36. doi:10.4103/picr.PICR_154_18

Nevid J. Psychology: Concepts and Applications. Wadworth, 2013.

By Kendra Cherry, MSEd Kendra Cherry, MS, is a psychosocial rehabilitation specialist, psychology educator, and author of the "Everything Psychology Book."

Research Hypothesis In Psychology: Types, & Examples

Saul Mcleod, PhD

Editor-in-Chief for Simply Psychology

BSc (Hons) Psychology, MRes, PhD, University of Manchester

Saul Mcleod, PhD., is a qualified psychology teacher with over 18 years of experience in further and higher education. He has been published in peer-reviewed journals, including the Journal of Clinical Psychology.

Learn about our Editorial Process

Olivia Guy-Evans, MSc

Associate Editor for Simply Psychology

BSc (Hons) Psychology, MSc Psychology of Education

Olivia Guy-Evans is a writer and associate editor for Simply Psychology. She has previously worked in healthcare and educational sectors.

On This Page:

A research hypothesis, in its plural form “hypotheses,” is a specific, testable prediction about the anticipated results of a study, established at its outset. It is a key component of the scientific method .

Hypotheses connect theory to data and guide the research process towards expanding scientific understanding

Some key points about hypotheses:

  • A hypothesis expresses an expected pattern or relationship. It connects the variables under investigation.
  • It is stated in clear, precise terms before any data collection or analysis occurs. This makes the hypothesis testable.
  • A hypothesis must be falsifiable. It should be possible, even if unlikely in practice, to collect data that disconfirms rather than supports the hypothesis.
  • Hypotheses guide research. Scientists design studies to explicitly evaluate hypotheses about how nature works.
  • For a hypothesis to be valid, it must be testable against empirical evidence. The evidence can then confirm or disprove the testable predictions.
  • Hypotheses are informed by background knowledge and observation, but go beyond what is already known to propose an explanation of how or why something occurs.
Predictions typically arise from a thorough knowledge of the research literature, curiosity about real-world problems or implications, and integrating this to advance theory. They build on existing literature while providing new insight.

Types of Research Hypotheses

Alternative hypothesis.

The research hypothesis is often called the alternative or experimental hypothesis in experimental research.

It typically suggests a potential relationship between two key variables: the independent variable, which the researcher manipulates, and the dependent variable, which is measured based on those changes.

The alternative hypothesis states a relationship exists between the two variables being studied (one variable affects the other).

A hypothesis is a testable statement or prediction about the relationship between two or more variables. It is a key component of the scientific method. Some key points about hypotheses:

  • Important hypotheses lead to predictions that can be tested empirically. The evidence can then confirm or disprove the testable predictions.

In summary, a hypothesis is a precise, testable statement of what researchers expect to happen in a study and why. Hypotheses connect theory to data and guide the research process towards expanding scientific understanding.

An experimental hypothesis predicts what change(s) will occur in the dependent variable when the independent variable is manipulated.

It states that the results are not due to chance and are significant in supporting the theory being investigated.

The alternative hypothesis can be directional, indicating a specific direction of the effect, or non-directional, suggesting a difference without specifying its nature. It’s what researchers aim to support or demonstrate through their study.

Null Hypothesis

The null hypothesis states no relationship exists between the two variables being studied (one variable does not affect the other). There will be no changes in the dependent variable due to manipulating the independent variable.

It states results are due to chance and are not significant in supporting the idea being investigated.

The null hypothesis, positing no effect or relationship, is a foundational contrast to the research hypothesis in scientific inquiry. It establishes a baseline for statistical testing, promoting objectivity by initiating research from a neutral stance.

Many statistical methods are tailored to test the null hypothesis, determining the likelihood of observed results if no true effect exists.

This dual-hypothesis approach provides clarity, ensuring that research intentions are explicit, and fosters consistency across scientific studies, enhancing the standardization and interpretability of research outcomes.

Nondirectional Hypothesis

A non-directional hypothesis, also known as a two-tailed hypothesis, predicts that there is a difference or relationship between two variables but does not specify the direction of this relationship.

It merely indicates that a change or effect will occur without predicting which group will have higher or lower values.

For example, “There is a difference in performance between Group A and Group B” is a non-directional hypothesis.

Directional Hypothesis

A directional (one-tailed) hypothesis predicts the nature of the effect of the independent variable on the dependent variable. It predicts in which direction the change will take place. (i.e., greater, smaller, less, more)

It specifies whether one variable is greater, lesser, or different from another, rather than just indicating that there’s a difference without specifying its nature.

For example, “Exercise increases weight loss” is a directional hypothesis.

hypothesis

Falsifiability

The Falsification Principle, proposed by Karl Popper , is a way of demarcating science from non-science. It suggests that for a theory or hypothesis to be considered scientific, it must be testable and irrefutable.

Falsifiability emphasizes that scientific claims shouldn’t just be confirmable but should also have the potential to be proven wrong.

It means that there should exist some potential evidence or experiment that could prove the proposition false.

However many confirming instances exist for a theory, it only takes one counter observation to falsify it. For example, the hypothesis that “all swans are white,” can be falsified by observing a black swan.

For Popper, science should attempt to disprove a theory rather than attempt to continually provide evidence to support a research hypothesis.

Can a Hypothesis be Proven?

Hypotheses make probabilistic predictions. They state the expected outcome if a particular relationship exists. However, a study result supporting a hypothesis does not definitively prove it is true.

All studies have limitations. There may be unknown confounding factors or issues that limit the certainty of conclusions. Additional studies may yield different results.

In science, hypotheses can realistically only be supported with some degree of confidence, not proven. The process of science is to incrementally accumulate evidence for and against hypothesized relationships in an ongoing pursuit of better models and explanations that best fit the empirical data. But hypotheses remain open to revision and rejection if that is where the evidence leads.
  • Disproving a hypothesis is definitive. Solid disconfirmatory evidence will falsify a hypothesis and require altering or discarding it based on the evidence.
  • However, confirming evidence is always open to revision. Other explanations may account for the same results, and additional or contradictory evidence may emerge over time.

We can never 100% prove the alternative hypothesis. Instead, we see if we can disprove, or reject the null hypothesis.

If we reject the null hypothesis, this doesn’t mean that our alternative hypothesis is correct but does support the alternative/experimental hypothesis.

Upon analysis of the results, an alternative hypothesis can be rejected or supported, but it can never be proven to be correct. We must avoid any reference to results proving a theory as this implies 100% certainty, and there is always a chance that evidence may exist which could refute a theory.

How to Write a Hypothesis

  • Identify variables . The researcher manipulates the independent variable and the dependent variable is the measured outcome.
  • Operationalized the variables being investigated . Operationalization of a hypothesis refers to the process of making the variables physically measurable or testable, e.g. if you are about to study aggression, you might count the number of punches given by participants.
  • Decide on a direction for your prediction . If there is evidence in the literature to support a specific effect of the independent variable on the dependent variable, write a directional (one-tailed) hypothesis. If there are limited or ambiguous findings in the literature regarding the effect of the independent variable on the dependent variable, write a non-directional (two-tailed) hypothesis.
  • Make it Testable : Ensure your hypothesis can be tested through experimentation or observation. It should be possible to prove it false (principle of falsifiability).
  • Clear & concise language . A strong hypothesis is concise (typically one to two sentences long), and formulated using clear and straightforward language, ensuring it’s easily understood and testable.

Consider a hypothesis many teachers might subscribe to: students work better on Monday morning than on Friday afternoon (IV=Day, DV= Standard of work).

Now, if we decide to study this by giving the same group of students a lesson on a Monday morning and a Friday afternoon and then measuring their immediate recall of the material covered in each session, we would end up with the following:

  • The alternative hypothesis states that students will recall significantly more information on a Monday morning than on a Friday afternoon.
  • The null hypothesis states that there will be no significant difference in the amount recalled on a Monday morning compared to a Friday afternoon. Any difference will be due to chance or confounding factors.

More Examples

  • Memory : Participants exposed to classical music during study sessions will recall more items from a list than those who studied in silence.
  • Social Psychology : Individuals who frequently engage in social media use will report higher levels of perceived social isolation compared to those who use it infrequently.
  • Developmental Psychology : Children who engage in regular imaginative play have better problem-solving skills than those who don’t.
  • Clinical Psychology : Cognitive-behavioral therapy will be more effective in reducing symptoms of anxiety over a 6-month period compared to traditional talk therapy.
  • Cognitive Psychology : Individuals who multitask between various electronic devices will have shorter attention spans on focused tasks than those who single-task.
  • Health Psychology : Patients who practice mindfulness meditation will experience lower levels of chronic pain compared to those who don’t meditate.
  • Organizational Psychology : Employees in open-plan offices will report higher levels of stress than those in private offices.
  • Behavioral Psychology : Rats rewarded with food after pressing a lever will press it more frequently than rats who receive no reward.

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On the scope of scientific hypotheses

William hedley thompson.

1 Department of Applied Information Technology, University of Gothenburg, Gothenburg, Sweden

2 Institute of Neuroscience and Physiology, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden

3 Department of Pedagogical, Curricular and Professional Studies, Faculty of Education, University of Gothenburg, Gothenburg, Sweden

4 Department of Clinical Neuroscience, Karolinska Institutet, Stockholm, Sweden

Associated Data

This article has no additional data.

Hypotheses are frequently the starting point when undertaking the empirical portion of the scientific process. They state something that the scientific process will attempt to evaluate, corroborate, verify or falsify. Their purpose is to guide the types of data we collect, analyses we conduct, and inferences we would like to make. Over the last decade, metascience has advocated for hypotheses being in preregistrations or registered reports, but how to formulate these hypotheses has received less attention. Here, we argue that hypotheses can vary in specificity along at least three independent dimensions: the relationship, the variables, and the pipeline. Together, these dimensions form the scope of the hypothesis. We demonstrate how narrowing the scope of a hypothesis in any of these three ways reduces the hypothesis space and that this reduction is a type of novelty. Finally, we discuss how this formulation of hypotheses can guide researchers to formulate the appropriate scope for their hypotheses and should aim for neither too broad nor too narrow a scope. This framework can guide hypothesis-makers when formulating their hypotheses by helping clarify what is being tested, chaining results to previous known findings, and demarcating what is explicitly tested in the hypothesis.

1.  Introduction

Hypotheses are an important part of the scientific process. However, surprisingly little attention is given to hypothesis-making compared to other skills in the scientist's skillset within current discussions aimed at improving scientific practice. Perhaps this lack of emphasis is because the formulation of the hypothesis is often considered less relevant, as it is ultimately the scientific process that will eventually decide the veracity of the hypothesis. However, there are more hypotheses than scientific studies as selection occurs at various stages: from funder selection and researcher's interests. So which hypotheses are worthwhile to pursue? Which hypotheses are the most effective or pragmatic for extending or enhancing our collective knowledge? We consider the answer to these questions by discussing how broad or narrow a hypothesis can or should be (i.e. its scope).

We begin by considering that the two statements below are both hypotheses and vary in scope:

  • H 1 : For every 1 mg decrease of x , y will increase by, on average, 2.5 points.
  • H 2 : Changes in x 1 or x 2 correlate with y levels in some way.

Clearly, the specificity of the two hypotheses is very different. H 1 states a precise relationship between two variables ( x and y ), while H 2 specifies a vaguer relationship and does not specify which variables will show the relationship. However, they are both still hypotheses about how x and y relate to each other. This claim of various degrees of the broadness of hypotheses is, in and of itself, not novel. In Epistemetrics, Rescher [ 1 ], while drawing upon the physicist Duhem's work, develops what he calls Duhem's Law. This law considers a trade-off between certainty or precision in statements about physics when evaluating them. Duhem's Law states that narrower hypotheses, such as H 1 above, are more precise but less likely to be evaluated as true than broader ones, such as H 2 above. Similarly, Popper, when discussing theories, describes the reverse relationship between content and probability of a theory being true, i.e. with increased content, there is a decrease in probability and vice versa [ 2 ]. Here we will argue that it is important that both H 1 and H 2 are still valid scientific hypotheses, and their appropriateness depends on certain scientific questions.

The question of hypothesis scope is relevant since there are multiple recent prescriptions to improve science, ranging from topics about preregistrations [ 3 ], registered reports [ 4 ], open science [ 5 ], standardization [ 6 ], generalizability [ 7 ], multiverse analyses [ 8 ], dataset reuse [ 9 ] and general questionable research practices [ 10 ]. Within each of these issues, there are arguments to demarcate between confirmatory and exploratory research or normative prescriptions about how science should be done (e.g. science is ‘bad’ or ‘worse’ if code/data are not open). Despite all these discussions and improvements, much can still be done to improve hypothesis-making. A recent evaluation of preregistered studies in psychology found that over half excluded the preregistered hypotheses [ 11 ]. Further, evaluations of hypotheses in ecology showed that most hypotheses are not explicitly stated [ 12 , 13 ]. Other research has shown that obfuscated hypotheses are more prevalent in retracted research [ 14 ]. There have been recommendations for simpler hypotheses in psychology to avoid misinterpretations and misspecifications [ 15 ]. Finally, several evaluations of preregistration practices have found that a significant proportion of articles do not abide by their stated hypothesis or add additional hypotheses [ 11 , 16 – 18 ]. In sum, while multiple efforts exist to improve scientific practice, our hypothesis-making could improve.

One of our intentions is to provide hypothesis-makers with tools to assist them when making hypotheses. We consider this useful and timely as, with preregistrations becoming more frequent, the hypothesis-making process is now open and explicit . However, preregistrations are difficult to write [ 19 ], and preregistered articles can change or omit hypotheses [ 11 ] or they are vague and certain degrees of freedom hard to control for [ 16 – 18 ]. One suggestion has been to do less confirmatory research [ 7 , 20 ]. While we agree that all research does not need to be confirmatory, we also believe that not all preregistrations of confirmatory work must test narrow hypotheses. We think there is a possible point of confusion that the specificity in preregistrations, where researcher degrees of freedom should be stated, necessitates the requirement that the hypothesis be narrow. Our belief that this confusion is occurring is supported by the study Akker et al . [ 11 ] where they found that 18% of published psychology studies changed their preregistered hypothesis (e.g. its direction), and 60% of studies selectively reported hypotheses in some way. It is along these lines that we feel the framework below can be useful to help formulate appropriate hypotheses to mitigate these identified issues.

We consider this article to be a discussion of the researcher's different choices when formulating hypotheses and to help link hypotheses over time. Here we aim to deconstruct what aspects there are in the hypothesis about their specificity. Throughout this article, we intend to be neutral to many different philosophies of science relating to the scientific method (i.e. how one determines the veracity of a hypothesis). Our idea of neutrality here is that whether a researcher adheres to falsification, verification, pragmatism, or some other philosophy of science, then this framework can be used when formulating hypotheses. 1

The framework this article advocates for is that there are (at least) three dimensions that hypotheses vary along regarding their narrowness and broadness: the selection of relationships, variables, and pipelines. We believe this discussion is fruitful for the current debate regarding normative practices as some positions make, sometimes implicit, commitments about which set of hypotheses the scientific community ought to consider good or permissible. We proceed by outlining a working definition of ‘scientific hypothesis' and then discuss how it relates to theory. Then, we justify how hypotheses can vary along the three dimensions. Using this framework, we then discuss the scopes in relation to appropriate hypothesis-making and an argument about what constitutes a scientifically novel hypothesis. We end the article with practical advice for researchers who wish to use this framework.

2.  The scientific hypothesis

In this section, we will describe a functional and descriptive role regarding how scientists use hypotheses. Jeong & Kwon [ 21 ] investigated and summarized the different uses the concept of ‘hypothesis’ had in philosophical and scientific texts. They identified five meanings: assumption, tentative explanation, tentative cause, tentative law, and prediction. Jeong & Kwon [ 21 ] further found that researchers in science and philosophy used all the different definitions of hypotheses, although there was some variance in frequency between fields. Here we see, descriptively , that the way researchers use the word ‘hypothesis’ is diverse and has a wide range in specificity and function. However, whichever meaning a hypothesis has, it aims to be true, adequate, accurate or useful in some way.

Not all hypotheses are ‘scientific hypotheses'. For example, consider the detective trying to solve a crime and hypothesizing about the perpetrator. Such a hypothesis still aims to be true and is a tentative explanation but differs from the scientific hypothesis. The difference is that the researcher, unlike the detective, evaluates the hypothesis with the scientific method and submits the work for evaluation by the scientific community. Thus a scientific hypothesis entails a commitment to evaluate the statement with the scientific process . 2 Additionally, other types of hypotheses can exist. As discussed in more detail below, scientific theories generate not only scientific hypotheses but also contain auxiliary hypotheses. The latter refers to additional assumptions considered to be true and not explicitly evaluated. 3

Next, the scientific hypothesis is generally made antecedent to the evaluation. This does not necessitate that the event (e.g. in archaeology) or the data collection (e.g. with open data reuse) must be collected before the hypothesis is made, but that the evaluation of the hypothesis cannot happen before its formulation. This claim state does deny the utility of exploratory hypothesis testing of post hoc hypotheses (see [ 25 ]). However, previous results and exploration can generate new hypotheses (e.g. via abduction [ 22 , 26 – 28 ], which is the process of creating hypotheses from evidence), which is an important part of science [ 29 – 32 ], but crucially, while these hypotheses are important and can be the conclusion of exploratory work, they have yet to be evaluated (by whichever method of choice). Hence, they still conform to the antecedency requirement. A further way to justify the antecedency is seen in the practice of formulating a post hoc hypothesis, and considering it to have been evaluated is seen as a questionable research practice (known as ‘hypotheses after results are known’ or HARKing [ 33 ]). 4

While there is a varying range of specificity, is the hypothesis a critical part of all scientific work, or is it reserved for some subset of investigations? There are different opinions regarding this. Glass and Hall, for example, argue that the term only refers to falsifiable research, and model-based research uses verification [ 36 ]. However, this opinion does not appear to be the consensus. Osimo and Rumiati argue that any model based on or using data is never wholly free from hypotheses, as hypotheses can, even implicitly, infiltrate the data collection [ 37 ]. For our definition, we will consider hypotheses that can be involved in different forms of scientific evaluation (i.e. not just falsification), but we do not exclude the possibility of hypothesis-free scientific work.

Finally, there is a debate about whether theories or hypotheses should be linguistic or formal [ 38 – 40 ]. Neither side in this debate argues that verbal or formal hypotheses are not possible, but instead, they discuss normative practices. Thus, for our definition, both linguistic and formal hypotheses are considered viable.

Considering the above discussion, let us summarize the scientific process and the scientific hypothesis: a hypothesis guides what type of data are sampled and what analysis will be done. With the new observations, evidence is analysed or quantified in some way (often using inferential statistics) to judge the hypothesis's truth value, utility, credibility, or likelihood. The following working definition captures the above:

  • Scientific hypothesis : an implicit or explicit statement that can be verbal or formal. The hypothesis makes a statement about some natural phenomena (via an assumption, explanation, cause, law or prediction). The scientific hypothesis is made antecedent to performing a scientific process where there is a commitment to evaluate it.

For simplicity, we will only use the term ‘hypothesis’ for ‘scientific hypothesis' to refer to the above definition for the rest of the article except when it is necessary to distinguish between other types of hypotheses. Finally, this definition could further be restrained in multiple ways (e.g. only explicit hypotheses are allowed, or assumptions are never hypotheses). However, if the definition is more (or less) restrictive, it has little implication for the argument below.

3.  The hypothesis, theory and auxiliary assumptions

While we have a definition of the scientific hypothesis, we have yet to link it with how it relates to scientific theory, where there is frequently some interconnection (i.e. a hypothesis tests a scientific theory). Generally, for this paper, we believe our argument applies regardless of how scientific theory is defined. Further, some research lacks theory, sometimes called convenience or atheoretical studies [ 41 ]. Here a hypothesis can be made without a wider theory—and our framework fits here too. However, since many consider hypotheses to be defined or deducible from scientific theory, there is an important connection between the two. Therefore, we will briefly clarify how hypotheses relate to common formulations of scientific theory.

A scientific theory is generally a set of axioms or statements about some objects, properties and their relations relating to some phenomena. Hypotheses can often be deduced from the theory. Additionally, a theory has boundary conditions. The boundary conditions specify the domain of the theory stating under what conditions it applies (e.g. all things with a central neural system, humans, women, university teachers) [ 42 ]. Boundary conditions of a theory will consequently limit all hypotheses deduced from the theory. For example, with a boundary condition ‘applies to all humans’, then the subsequent hypotheses deduced from the theory are limited to being about humans. While this limitation of the hypothesis by the theory's boundary condition exists, all the considerations about a hypothesis scope detailed below still apply within the boundary conditions. Finally, it is also possible (depending on the definition of scientific theory) for a hypothesis to test the same theory under different boundary conditions. 5

The final consideration relating scientific theory to scientific hypotheses is auxiliary hypotheses. These hypotheses are theories or assumptions that are considered true simultaneously with the theory. Most philosophies of science from Popper's background knowledge [ 24 ], Kuhn's paradigms during normal science [ 44 ], and Laktos' protective belt [ 45 ] all have their own versions of this auxiliary or background information that is required for the hypothesis to test the theory. For example, Meelh [ 46 ] auxiliary theories/assumptions are needed to go from theoretical terms to empirical terms (e.g. neural activity can be inferred from blood oxygenation in fMRI research or reaction time to an indicator of cognition) and auxiliary theories about instruments (e.g. the experimental apparatus works as intended) and more (see also Other approaches to categorizing hypotheses below). As noted in the previous section, there is a difference between these auxiliary hypotheses, regardless of their definition, and the scientific hypothesis defined above. Recall that our definition of the scientific hypothesis included a commitment to evaluate it. There are no such commitments with auxiliary hypotheses, but rather they are assumed to be correct to test the theory adequately. This distinction proves to be important as auxiliary hypotheses are still part of testing a theory but are separate from the hypothesis to be evaluated (discussed in more detail below).

4.  The scope of hypotheses

In the scientific hypothesis section, we defined the hypothesis and discussed how it relates back to the theory. In this section, we want to defend two claims about hypotheses:

  • (A1) Hypotheses can have different scopes . Some hypotheses are narrower in their formulation, and some are broader.
  • (A2) The scope of hypotheses can vary along three dimensions relating to relationship selection , variable selection , and pipeline selection .

A1 may seem obvious, but it is important to establish what is meant by narrower and broader scope. When a hypothesis is very narrow, it is specific. For example, it might be specific about the type of relationship between some variables. In figure 1 , we make four different statements regarding the relationship between x and y . The narrowest hypothesis here states ‘there is a positive linear relationship with a magnitude of 0.5 between x and y ’ ( figure 1 a ), and the broadest hypothesis states ‘there is a relationship between x and y ’ ( figure 1 d ). Note that many other hypotheses are possible that are not included in this example (such as there being no relationship).

An external file that holds a picture, illustration, etc.
Object name is rsos230607f01.jpg

Examples of narrow and broad hypotheses between x and y . Circles indicate a set of possible relationships with varying slopes that can pivot or bend.

We see that the narrowest of these hypotheses claims a type of relationship (linear), a direction of the relationship (positive) and a magnitude of the relationship (0.5). As the hypothesis becomes broader, the specific magnitude disappears ( figure 1 b ), the relationship has additional options than just being linear ( figure 1 c ), and finally, the direction of the relationship disappears. Crucially, all the examples in figure 1 can meet the above definition of scientific hypotheses. They are all statements that can be evaluated with the same scientific method. There is a difference between these statements, though— they differ in the scope of the hypothesis . Here we have justified A1.

Within this framework, when we discuss whether a hypothesis is narrower or broader in scope, this is a relation between two hypotheses where one is a subset of the other. This means that if H 1 is narrower than H 2 , and if H 1 is true, then H 2 is also true. This can be seen in figure 1 a–d . Suppose figure 1 a , the narrowest of all the hypotheses, is true. In that case, all the other broader statements are also true (i.e. a linear correlation of 0.5 necessarily entails that there is also a positive linear correlation, a linear correlation, and some relationship). While this property may appear trivial, it entails that it is only possible to directly compare the hypothesis scope between two hypotheses (i.e. their broadness or narrowness) where one is the subset of the other. 6

4.1. Sets, disjunctions and conjunctions of elements

The above restraint defines the scope as relations between sets. This property helps formalize the framework of this article. Below, when we discuss the different dimensions that can impact the scope, these become represented as a set. Each set contains elements. Each element is a permissible situation that allows the hypothesis to be accepted. We denote elements as lower case with italics (e.g. e 1 , e 2 , e 3 ) and sets as bold upper case (e.g. S ). Each of the three different dimensions discussed below will be formalized as sets, while the total number of elements specifies their scope.

Let us reconsider the above restraint about comparing hypotheses as narrower or broader. This can be formally shown if:

  • e 1 , e 2 , e 3 are elements of S 1 ; and
  • e 1 and e 2 are elements of S 2 ,

then S 2 is narrower than S 1 .

Each element represents specific propositions that, if corroborated, would support the hypothesis. Returning to figure 1 a , b , the following statements apply to both:

  • ‘There is a positive linear relationship between x and y with a slope of 0.5’.

Whereas the following two apply to figure 1 b but not figure 1 a :

  • ‘There is a positive linear relationship between x and y with a slope of 0.4’ ( figure 1 b ).
  • ‘There is a positive linear relationship between x and y with a slope of 0.3’ ( figure 1 b ).

Figure 1 b allows for a considerably larger number of permissible situations (which is obvious as it allows for any positive linear relationship). When formulating the hypothesis in figure 1 b , we do not need to specify every single one of these permissible relationships. We can simply specify all possible positive slopes, which entails the set of permissible elements it includes.

That broader hypotheses have more elements in their sets entails some important properties. When we say S contains the elements e 1 , e 2 , and e 3 , the hypothesis is corroborated if e 1 or e 2 or e 3 is the case. This means that the set requires only one of the elements to be corroborated for the hypothesis to be considered correct (i.e. the positive linear relationship needs to be 0.3 or 0.4 or 0.5). Contrastingly, we will later see cases when conjunctions of elements occur (i.e. both e 1 and e 2 are the case). When a conjunction occurs, in this formulation, the conjunction itself becomes an element in the set (i.e. ‘ e 1 and e 2 ’ is a single element). Figure 2 illustrates how ‘ e 1 and e 2 ’ is narrower than ‘ e 1 ’, and ‘ e 1 ’ is narrower than ‘ e 1 or e 2 ’. 7 This property relating to the conjunction being narrower than individual elements is explained in more detail in the pipeline selection section below.

An external file that holds a picture, illustration, etc.
Object name is rsos230607f02.jpg

Scope as sets. Left : four different sets (grey, red, blue and purple) showing different elements which they contain. Right : a list of each colour explaining which set is a subset of the other (thereby being ‘narrower’).

4.2. Relationship selection

We move to A2, which is to show the different dimensions that a hypothesis scope can vary along. We have already seen an example of the first dimension of a hypothesis in figure 1 , the relationship selection . Let R denote the set of all possible configurations of relationships that are permissible for the hypothesis to be considered true. For example, in the narrowest formulation above, there was one allowed relationship for the hypothesis to be true. Consequently, the size of R (denoted | R |) is one. As discussed above, in the second narrowest formulation ( figure 1 b ), R has more possible relationships where it can still be considered true:

  • r 1 = ‘a positive linear relationship of 0.1’
  • r 2 = ‘a positive linear relationship of 0.2’
  • r 3 = ‘a positive linear relationship of 0.3’.

Additionally, even broader hypotheses will be compatible with more types of relationships. In figure 1 c , d , nonlinear and negative relationships are also possible relationships included in R . For this broader statement to be affirmed, more elements are possible to be true. Thus if | R | is greater (i.e. contains more possible configurations for the hypothesis to be true), then the hypothesis is broader. Thus, the scope of relating to the relationship selection is specified by | R |. Finally, if |R H1 | > |R H2 | , then H 1 is broader than H 2 regarding the relationship selection.

Figure 1 is an example of the relationship narrowing. That the relationship became linear is only an example and does not necessitate a linear relationship or that this scope refers only to correlations. An alternative example of a relationship scope is a broad hypothesis where there is no knowledge about the distribution of some data. In such situations, one may assume a uniform relationship or a Cauchy distribution centred at zero. Over time the specific distribution can be hypothesized. Thereafter, the various parameters of the distribution can be hypothesized. At each step, the hypothesis of the distribution gets further specified to narrower formulations where a smaller set of possible relationships are included (see [ 47 , 48 ] for a more in-depth discussion about how specific priors relate to more narrow tests). Finally, while figure 1 was used to illustrate the point of increasingly narrow relationship hypotheses, it is more likely to expect the narrowest relationship, within fields such as psychology, to have considerable uncertainty and be formulated with confidence or credible intervals (i.e. we will rarely reach point estimates).

4.3. Variable selection

We have demonstrated that relationship selection can affect the scope of a hypothesis. Additionally, at least two other dimensions can affect the scope of a hypothesis: variable selection and pipeline selection . The variable selection in figure 1 was a single bivariate relationship (e.g. x 's relationship with y ). However, it is not always the case that we know which variables will be involved. For example, in neuroimaging, we can be confident that one or more brain regions will be processing some information following a stimulus. Still, we might not be sure which brain region(s) this will be. Consequently, our hypothesis becomes broader because we have selected more variables. The relationship selection may be identical for each chosen variable, but the variable selection becomes broader. We can consider the following three hypotheses to be increasing in their scope:

  • H 1 : x relates to y with relationship R .
  • H 2 : x 1 or x 2 relates to y with relationship R .
  • H 3 : x 1 or x 2 or x 3 relates to y with relationship R .

For H 1 –H 3 above, we assume that R is the same. Further, we assume that there is no interaction between these variables.

In the above examples, we have multiple x ( x 1 , x 2 , x 3 , … , x n ). Again, we can symbolize the variable selection as a non-empty set XY , containing either a single variable or many variables. Our motivation for designating it XY is that the variable selection can include multiple possibilities for both the independent variable ( x ) and the dependent variable ( y ). Like with relationship selection, we can quantify the broadness between two hypotheses with the size of the set XY . Consequently, | XY | denotes the total scope concerning variable selection. Thus, in the examples above | XY H1 | < | XY H2 | < | XY H3 |. Like with relationship selection, hypotheses that vary in | XY | still meet the definition of a hypothesis. 8

An obvious concern for many is that a broader XY is much easier to evaluate as correct. Generally, when | XY 1 | > | XY 2 |, there is a greater chance of spurious correlations when evaluating XY 1 . This concern is an issue relating to the evaluation of hypotheses (e.g. applying statistics to the evaluation), which will require additional assumptions relating to how to evaluate the hypotheses. Strategies to deal with this apply some correction or penalization for multiple statistical testing [ 49 ] or partial pooling and regularizing priors [ 50 , 51 ]. These strategies aim to evaluate a broader variable selection ( x 1 or x 2 ) on equal or similar terms to a narrow variable selection ( x 1 ).

4.4. Pipeline selection

Scientific studies require decisions about how to perform the analysis. This scope considers transformations applied to the raw data ( XY raw ) to achieve some derivative ( XY ). These decisions can also involve selection procedures that drop observations deemed unreliable, standardizing, correcting confounding variables, or different philosophies. We can call the array of decisions and transformations used as the pipeline . A hypothesis varies in the number of pipelines:

  • H 1 : XY has a relationship(s) R with pipeline p 1 .
  • H 2 : XY has a relationship(s) R with pipeline p 1 or pipeline p 2 .
  • H 3 : XY has a relationship(s) R with pipeline p 1 or pipeline p 2 , or pipeline p 3 .

Importantly, the pipeline here considers decisions regarding how the hypothesis shapes the data collection and transformation. We do not consider this to include decisions made regarding the assumptions relating to the statistical inference as those relate to operationalizing the evaluation of the hypothesis and not part of the hypothesis being evaluated (these assumptions are like auxiliary hypotheses, which are assumed to be true but not explicitly evaluated).

Like with variable selection ( XY ) and relationship selection ( R ), we can see that pipelines impact the scope of hypotheses. Again, we can symbolize the pipeline selection with a set P . As previously, | P | will denote the dimension of the pipeline selection. In the case of pipeline selection, we are testing the same variables, looking for the same relationship, but processing the variables or relationships with different pipelines to evaluate the relationship. Consequently, | P H1 | < | P H2 | < | P H3 |.

These issues regarding pipelines have received attention as the ‘garden of forking paths' [ 52 ]. Here, there are calls for researchers to ensure that their entire pipeline has been specified. Additionally, recent work has highlighted the diversity of results based on multiple analytical pipelines [ 53 , 54 ]. These results are often considered a concern, leading to calls that results should be pipeline resistant.

The wish for pipeline-resistant methods entails that hypotheses, in their narrowest form, are possible for all pipelines. Consequently, a narrower formulation will entail that this should not impact the hypothesis regardless of which pipeline is chosen. Thus the conjunction of pipelines is narrower than single pipelines. Consider the following three scenarios:

  • H 3 : XY has a relationship(s) R with pipeline p 1 and pipeline p 2 .

In this instance, since H 1 is always true if H 3 is true, thus H 3 is a narrower formulation than H 1 . Consequently, | P H3 | < | P H1 | < | P H2 |. Decreasing the scope of the pipeline dimension also entails the increase in conjunction of pipelines (i.e. creating pipeline-resistant methods) rather than just the reduction of disjunctional statements.

4.5. Combining the dimensions

In summary, we then have three different dimensions that independently affect the scope of the hypothesis. We have demonstrated the following general claim regarding hypotheses:

  • The variables XY have a relationship R with pipeline P .

And that the broadness and narrowness of a hypothesis depend on how large the three sets XY , R and P are. With this formulation, we can conclude that hypotheses have a scope that can be determined with a 3-tuple argument of (| R |, | XY |, | P |).

While hypotheses can be formulated along these three dimensions and generally aim to be reduced, it does not entail that these dimensions behave identically. For example, the relationship dimensions aim to reduce the number of elements as far as possible (e.g. to an interval). Contrastingly, for both variables and pipeline, the narrower hypothesis can reduce to single variables/pipelines or become narrower still and become conjunctions where all variables/pipelines need to corroborate the hypothesis (i.e. regardless of which method one follows, the hypothesis is correct).

5.  Additional possible dimensions

No commitment is being made about the exhaustive nature of there only being three dimensions that specify the hypothesis scope. Other dimensions may exist that specify the scope of a hypothesis. For example, one might consider the pipeline dimension as two different dimensions. The first would consider the experimental pipeline dimension regarding all variables relating to the experimental setup to collect data, and the latter would be the analytical pipeline dimension regarding the data analysis of any given data snapshot. Another possible dimension is adding the number of situations or contexts under which the hypothesis is valid. For example, any restraint such as ‘in a vacuum’, ‘under the speed of light’, or ‘in healthy human adults' could be considered an additional dimension of the hypothesis. There is no objection to whether these should be additional dimensions of the hypothesis. However, as stated above, these usually follow from the boundary conditions of the theory.

6.  Specifying the scope versus assumptions

We envision that this framework can help hypothesis-makers formulate hypotheses (in research plans, registered reports, preregistrations etc.). Further, using this framework while formulating hypotheses can help distinguish between auxiliary hypotheses and parts of the scientific hypothesis being tested. When writing preregistrations, it can frequently occur that some step in the method has two alternatives (e.g. a preprocessing step), and there is not yet reason to choose one over the other, and the researcher needs to make a decision. These following scenarios are possible:

  • 1. Narrow pipeline scope . The researcher evaluates the hypothesis with both pipeline variables (i.e. H holds for both p 1 and p 2 where p 1 and p 2 can be substituted with each other in the pipeline).
  • 2. Broad pipeline scope. The researcher evaluates the hypothesis with both pipeline variables, and only one needs to be correct (i.e. H holds for either p 1 or p 2 where p 1 and p 2 can be substituted with each other in the pipeline). The result of this experiment may help motivate choosing either p 1 or p 2 in future studies.
  • 3. Auxiliary hypothesis. Based on some reason (e.g. convention), the researcher assumes p 1 and evaluates H assuming p 1 is true.

Here we see that the same pipeline step can be part of either the auxiliary hypotheses or the pipeline scope. This distinction is important because if (3) is chosen, the decision becomes an assumption that is not explicitly tested by the hypothesis. Consequently, a researcher confident in the hypothesis may state that the auxiliary hypothesis p 1 was incorrect, and they should retest their hypothesis using different assumptions. In the cases where this decision is part of the pipeline scope, the hypothesis is intertwined with this decision, removing the eventual wiggle-room to reject auxiliary hypotheses that were assumed. Furthermore, starting with broader pipeline hypotheses that gradually narrow down can lead to a more well-motivated protocol for approaching the problem. Thus, this framework can help researchers while writing their hypotheses in, for example, preregistrations because they can consider when they are committing to a decision, assuming it, or when they should perhaps test a broader hypothesis with multiple possible options (discussed in more detail in §11 below).

7.  The reduction of scope in hypothesis space

Having established that different scopes of a hypothesis are possible, we now consider how the hypotheses change over time. In this section, we consider how the scope of the hypothesis develops ideally within science.

Consider a new research question. A large number of hypotheses are possible. Let us call this set of all possible hypotheses the hypothesis space . Hypotheses formulated within this space can be narrower or broader based on the dimensions discussed previously ( figure 3 ).

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Example of hypothesis space. The hypothesis scope is expressed as cuboids in three dimensions (relationship ( R ), variable ( XY ), pipeline ( P )). The hypothesis space is the entire possible space within the three dimensions. Three hypotheses are shown in the hypothesis space (H 1 , H 2 , H 3 ). H 2 and H 3 are subsets of H 1 .

After the evaluation of the hypothesis with the scientific process, the hypothesis will be accepted or rejected. 9 The evaluation could be done through falsification or via verification, depending on the philosophy of science commitments. Thereafter, other narrower formulations of the hypothesis can be formulated by reducing the relationship, variable or pipeline scope. If a narrower hypothesis is accepted, more specific details about the subject matter are known, or a theory has been refined in greater detail. A narrower hypothesis will entail a more specific relationship, variable or pipeline detailed in the hypothesis. Consequently, hypotheses linked to each other in this way will become narrower over time along one or more dimensions. Importantly, considering that the conjunction of elements is narrower than single elements for pipelines and variables, this process of narrower hypotheses will lead to more general hypotheses (i.e. they have to be applied in all conditions and yield less flexibility when they do not apply). 10

Considering that the scopes of hypotheses were defined as sets above, some properties can be deduced from this framework about how narrower hypotheses relate to broader hypotheses. Let us consider three hypotheses (H 1 , H 2 , and H 3 ; figure 3 ). H 2 and H 3 are non-overlapping subsets of H 1 . Thus H 2 and H 3 are both narrower in scope than H 1 . Thus the following is correct:

  • P1: If H 1 is false, then H 2 is false, and H 2 does not need to be evaluated.
  • P2: If H 2 is true, then the broader H 1 is true, and H 1 does not need to be evaluated.
  • P3: If H 1 is true and H 2 is false, some other hypothesis H 3 of similar scope to H 2 is possible.

For example, suppose H 1 is ‘there is a relationship between x and y ’, H 2 is ‘there is a positive relationship between x and y ’, and H 3 is ‘a negative relationship between x and y ’. In that case, it becomes apparent how each of these follows. 11 Logically, many deductions from set theory are possible but will not be explored here. Instead, we will discuss two additional consequences of hypothesis scopes: scientific novelty and applications for the researcher who formulates a hypothesis.

P1–P3 have been formulated as hypotheses being true or false. In practice, hypotheses are likely evaluated probabilistically (e.g. ‘H 1 is likely’ or ‘there is evidence in support of H 1 ’). In these cases, P1–P3 can be rephrased to account for this by substituting true/false with statements relating to evidence. For example, P2 could read: ‘If there is evidence in support of H 2 , then there is evidence in support of H 1 , and H 1 does not need to be evaluated’.

8.  Scientific novelty as the reduction of scope

Novelty is a key concept that repeatedly occurs in multiple aspects of the scientific enterprise, from funding to publishing [ 55 ]. Generally, scientific progress establishes novel results based on some new hypothesis. Consequently, the new hypothesis for the novel results must be narrower than previously established knowledge (i.e. the size of the scopes is reduced). Otherwise, the result is trivial and already known (see P2 above). Thus, scientific work is novel if the scientific process produces a result based on hypotheses with either a smaller | R |, | XY |, or | P | compared to previous work.

This framework of dimensions of the scope of a hypothesis helps to demarcate when a hypothesis and the subsequent result are novel. If previous studies have established evidence for R 1 (e.g. there is a positive relationship between x and y ), a hypothesis will be novel if and only if it is narrower than R 1 . Thus, if R 2 is narrower in scope than R 1 (i.e. | R 2 | < | R 1 |), R 2 is a novel hypothesis.

Consider the following example. Study 1 hypothesizes, ‘There is a positive relationship between x and y ’. It identifies a linear relationship of 0.6. Next, Study 2 hypothesizes, ‘There is a specific linear relationship between x and y that is 0.6’. Study 2 also identifies the relationship of 0.6. Since this was a narrower hypothesis, Study 2 is novel despite the same result. Frequently, researchers claim that they are the first to demonstrate a relationship. Being the first to demonstrate a relationship is not the final measure of novelty. Having a narrower hypothesis than previous researchers is a sign of novelty as it further reduces the hypothesis space.

Finally, it should be noted that novelty is not the only objective of scientific work. Other attributes, such as improving the certainty of a current hypothesis (e.g. through replications), should not be overlooked. Additional scientific explanations and improved theories are other aspects. Additionally, this definition of novelty relating to hypothesis scope does not exclude other types of novelty (e.g. new theories or paradigms).

9.  How broad should a hypothesis be?

Given the previous section, it is elusive to conclude that the hypothesis should be as narrow as possible as it entails maximal knowledge gain and scientific novelty when formulating hypotheses. Indeed, many who advocate for daring or risky tests seem to hold this opinion. For example, Meehl [ 46 ] argues that we should evaluate theories based on point (or interval) prediction, which would be compatible with very narrow versions of relationships. We do not necessarily think that this is the most fruitful approach. In this section, we argue that hypotheses should aim to be narrower than current knowledge , but too narrow may be problematic .

Let us consider the idea of confirmatory analyses. These studies will frequently keep the previous hypothesis scopes regarding P and XY but aim to become more specific regarding R (i.e. using the same method and the same variables to detect a more specific relationship). A very daring or narrow hypothesis is to minimize R to include the fewest possible relationships. However, it becomes apparent that simply pursuing specificness or daringness is insufficient for selecting relevant hypotheses. Consider a hypothetical scenario where a researcher believes virtual reality use leads people to overestimate the amount of exercise they have done. If unaware of previous studies on this project, an apt hypothesis is perhaps ‘increased virtual reality usage correlates with a less accuracy of reported exercise performed’ (i.e. R is broad). However, a more specific and more daring hypothesis would be to specify the relationship further. Thus, despite not knowing if there is a relationship at all, a more daring hypothesis could be: ‘for every 1 h of virtual reality usage, there will be, on average, a 0.5% decrease in the accuracy of reported exercise performed’ (i.e. R is narrow). We believe it would be better to establish the broader hypothesis in any scenario here for the first experiment. Otherwise, if we fail to confirm the more specific formulation, we could reformulate another equally narrow relative to the broader hypothesis. This process of tweaking a daring hypothesis could be pursued ad infinitum . Such a situation will neither quickly identify the true hypothesis nor effectively use limited research resources.

By first discounting a broader hypothesis that there is no relationship, it will automatically discard all more specific formulations of that relationship in the hypothesis space. Returning to figure 3 , it will be better to establish H 1 before attempting H 2 or H 3 to ensure the correct area in the hypothesis space is being investigated. To provide an analogy: when looking for a needle among hay, first identify which farm it is at, then which barn, then which haystack, then which part of the haystack it is at before we start picking up individual pieces of hay. Thus, it is preferable for both pragmatic and cost-of-resource reasons to formulate sufficiently broad hypotheses to navigate the hypothesis space effectively.

Conversely, formulating too broad a relationship scope in a hypothesis when we already have evidence for narrower scope would be superfluous research (unless the evidence has been called into question by, for example, not being replicated). If multiple studies have supported the hypothesis ‘there is a 20-fold decrease in mortality after taking some medication M’, it would be unnecessary to ask, ‘Does M have any effect?’.

Our conclusion is that the appropriate scope of a hypothesis, and its three dimensions, follow a Goldilocks-like principle where too broad is superfluous and not novel, while too narrow is unnecessary or wasteful. Considering the scope of one's hypothesis and how it relates to previous hypotheses' scopes ensures one is asking appropriate questions.

Finally, there has been a recent trend in psychology that hypotheses should be formal [ 38 , 56 – 60 ]. Formal theories are precise since they are mathematical formulations entailing that their interpretations are clear (non-ambiguous) compared to linguistic theories. However, this literature on formal theories often refers to ‘precise predictions’ and ‘risky testing’ while frequently referencing Meehl, who advocates for narrow hypotheses (e.g. [ 38 , 56 , 59 ]). While perhaps not intended by any of the proponents, one interpretation of some of these positions is that hypotheses derived from formal theories will be narrow hypotheses (i.e. the quality of being ‘precise’ can mean narrow hypotheses with risky tests and non-ambiguous interpretations simultaneously). However, the benefit from the clarity (non-ambiguity) that formal theories/hypotheses bring also applies to broad formal hypotheses as well. They can include explicit but formalized versions of uncertain relationships, multiple possible pipelines, and large sets of variables. For example, a broad formal hypothesis can contain a hyperparameter that controls which distribution the data fit (broad relationship scope), or a variable could represent a set of formalized explicit pipelines (broad pipeline scope) that will be tested. In each of these instances, it is possible to formalize non-ambiguous broad hypotheses from broad formal theories that do not yet have any justification for being overly narrow. In sum, our argumentation here stating that hypotheses should not be too narrow is not an argument against formal theories but rather that hypotheses (derived from formal theories) do not necessarily have to be narrow.

10.  Other approaches to categorizing hypotheses

The framework we present here is a way of categorizing hypotheses into (at least) three dimensions regarding the hypothesis scope, which we believe is accessible to researchers and help link scientific work over time while also trying to remain neutral with regard to a specific philosophy of science. Our proposal does not aim to be antagonistic or necessarily contradict other categorizing schemes—but we believe that our framework provides benefits.

One recent categorization scheme is the Theoretical (T), Auxiliary (A), Statistical (S) and Inferential (I) assumption model (together becoming the TASI model) [ 61 , 62 ]. Briefly, this model considers theory to generate theoretical hypotheses. To translate from theoretical unobservable terms (e.g. personality, anxiety, mass), auxiliary assumptions are needed to generate an empirical hypothesis. Statistical assumptions are often needed to test the empirical hypothesis (e.g. what is the distribution, is it skewed or not) [ 61 , 62 ]. Finally, additional inferential assumptions are needed to generalize to a larger population (e.g. was there a random and independent sampling from defined populations). The TASI model is insightful and helpful in highlighting the distance between a theory and the observation that would corroborate/contradict it. Part of its utility is to bring auxiliary hypotheses into the foreground, to improve comparisons between studies and improve theory-based interventions [ 63 , 64 ].

We do agree with the importance of being aware of or stating the auxiliary hypotheses, but there are some differences between the frameworks. First, the number of auxiliary assumptions in TASI can be several hundred [ 62 ], whereas our framework will consider some of them as part of the pipeline dimension. Consider the following four assumptions: ‘the inter-stimulus interval is between 2000 ms and 3000 ms', ‘the data will be z-transformed’, ‘subjects will perform correctly’, and ‘the measurements were valid’. According to the TASI model, all these will be classified similarly as auxiliary assumptions. Contrarily, within our framework, it is possible to consider the first two as part of the pipeline dimension and the latter two as auxiliary assumptions, and consequently, the first two become integrated as part of the hypothesis being tested and the latter two auxiliary assumptions. A second difference between the frameworks relates to non-theoretical studies (convenience, applied or atheoretical). Our framework allows for the possibility that the hypothesis space generated by theoretical and convenience studies can interact and inform each other within the same framework . Contrarily, in TASI, the theory assumptions no longer apply, and a different type of hypothesis model is needed; these assumptions must be replaced by another group of assumptions (where ‘substantive application assumptions' replace the T and the A, becoming SSI) [ 61 ]. Finally, part of our rationale for our framework is to be able to link and track hypotheses and hypothesis development together over time, so our classification scheme has different utility.

Another approach which has some similar utility to this framework is theory construction methodology (TCM) [ 57 ]. The similarity here is that TCM aims to be a practical guide to improve theory-making in psychology. It is an iterative process which relates theory, phenomena and data. Here hypotheses are not an explicit part of the model. However, what is designated as ‘proto theory’ could be considered a hypothesis in our framework as they are a product of abduction, shaping the theory space. Alternatively, what is deduced to evaluate the theory can also be considered a hypothesis. We consider both possible and that our framework can integrate with these two steps, especially since TCM does not have clear guidelines for how to do each step.

11.  From theory to practice: implementing this framework

We believe that many practising researchers can relate to many aspects of this framework. But, how can a researcher translate the above theoretical framework to their work? The utility of this framework lies in bringing these three scopes of a hypothesis together and explaining how each can be reduced. We believe researchers can use this framework to describe their current practices more clearly. Here we discuss how it can be helpful for researchers when formulating, planning, preregistering, and discussing the evaluation of their scientific hypotheses. These practical implications are brief, and future work can expand on the connection between the full interaction between hypothesis space and scope. Furthermore, both authors have the most experience in cognitive neuroscience, and some of the practical implications may revolve around this type of research and may not apply equally to other fields.

11.1. Helping to form hypotheses

Abduction, according to Peirce, is a hypothesis-making exercise [ 22 , 26 – 28 ]. Given some observations, a general testable explanation of the phenomena is formed. However, when making the hypothesis, this statement will have a scope (either explicitly or implicitly). Using our framework, the scope can become explicit. The hypothesis-maker can start with ‘The variables XY have a relationship R with pipeline P ’ as a scaffold to form the hypothesis. From here, the hypothesis-maker can ‘fill in the blanks’, explicitly adding each of the scopes. Thus, when making a hypothesis via abduction and using our framework, the hypothesis will have an explicit scope when it is made. By doing this, there is less chance that a formulated hypothesis is unclear, ambiguous, and needs amending at a later stage.

11.2. Assisting to clearly state hypotheses

A hypothesis is not just formulated but also communicated. Hypotheses are stated in funding applications, preregistrations, registered reports, and academic articles. Further, preregistered hypotheses are often omitted or changed in the final article [ 11 ], and hypotheses are not always explicitly stated in articles [ 12 ]. How can this framework help to make better hypotheses? Similar to the previous point, filling in the details of ‘The variables XY have a relationship R with pipeline P ’ is an explicit way to communicate the hypothesis. Thinking about each of these dimensions should entail an appropriate explicit scope and, hopefully, less variation between preregistered and reported hypotheses. The hypothesis does not need to be a single sentence, and details of XY and P will often be developed in the methods section of the text. However, using this template as a starting point can help ensure the hypothesis is stated, and the scope of all three dimensions has been communicated.

11.3. Helping to promote explicit and broad hypotheses instead of vague hypotheses

There is an important distinction between vague hypotheses and broad hypotheses, and this framework can help demarcate between them. A vague statement would be: ‘We will quantify depression in patients after treatment’. Here there is uncertainty relating to how the researcher will go about doing the experiment (i.e. how will depression be quantified?). However, a broad statement can be uncertain, but the uncertainty is part of the hypothesis: ‘Two different mood scales (S 1 or S 2 ) will be given to patients and test if only one (or both) changed after treatment’. This latter statement is transparently saying ‘S 1 or S 2 ’ is part of a broad hypothesis—the uncertainty is whether the two different scales are quantifying the same construct. We keep this uncertainty within the broad hypothesis, which will get evaluated, whereas a vague hypothesis has uncertainty as part of the interpretation of the hypothesis. This framework can be used when formulating hypotheses to help be broad (where needed) but not vague.

11.4. Which hypothesis should be chosen?

When considering the appropriate scope above, we argued for a Goldilocks-like principle of determining the hypothesis that is not too broad or too narrow. However, when writing, for example, a preregistration, how does one identify this sweet spot? There is no easy or definite universal answer to this question. However, one possible way is first to identify the XY , R , and P of previous hypotheses. From here, identify what a non-trivial step is to improve our knowledge of the research area. So, for example, could you be more specific about the exact nature of the relationship between the variables? Does the pipeline correspond to today's scientific standards, or were some suboptimal decisions made? Is there another population that you think the previous result also applies to? Do you think that maybe a more specific construct or subpopulation might explain the previous result? Could slightly different constructs (perhaps easier to quantify) be used to obtain a similar relationship? Are there even more constructs to which this relationship should apply simultaneously? Are you certain of the direction of the relationship? Answering affirmatively to any of these questions will likely make a hypothesis narrower and connect to previous research while being clear and explicit. Moreover, depending on the research question, answering any of these may be sufficiently narrow to be a non-trivial innovation. However, there are many other ways to make a hypothesis narrower than these guiding questions.

11.5. The confirmatory–exploratory continuum

Research is often dichotomized into confirmatory (testing a hypothesis) or exploratory (without a priori hypotheses). With this framework, researchers can consider how their research acts on some hypothesis space. Confirmatory and exploratory work has been defined in terms of how each interacts with the researcher's degrees of freedom (where confirmatory aims to reduce while exploratory utilizes them [ 30 ]). Both broad confirmatory and narrow exploratory research are possible using this definition and possible within this framework. How research interacts with the hypothesis space helps demarcate it. For example, if a hypothesis reduces the scope, it becomes more confirmatory, and trying to understand data given the current scope would be more exploratory work. This further could help demarcate when exploration is useful. Future theoretical work can detail how different types of research impact the hypothesis space in more detail.

11.6. Understanding when multiverse analyses are needed

Researchers writing a preregistration may face many degrees of freedom they have to choose from, and different researchers may motivate different choices. If, when writing such a preregistration, there appears to be little evidential support for certain degrees of freedom over others, the researcher is left with the option to either make more auxiliary assumptions or identify when an investigation into the pipeline scope is necessary by conducting a multiverse analysis that tests the impact of the different degrees of freedom on the result (see [ 8 ]). Thus, when applying this framework to explicitly state what pipeline variables are part of the hypothesis or an auxiliary assumption, the researcher can identify when it might be appropriate to conduct a multiverse analysis because they are having difficulty formulating hypotheses.

11.7. Describing novelty

Academic journals and research funders often ask for novelty, but the term ‘novelty’ can be vague and open to various interpretations [ 55 ]. This framework can be used to help justify the novelty of research. For example, consider a scenario where a previous study has established a psychological construct (e.g. well-being) that correlates with a certain outcome measure (e.g. long-term positive health outcomes). This framework can be used to explicitly justify novelty by (i) providing a more precise understanding of the relationship (e.g. linear or linear–plateau) or (ii) identifying more specific variables related to well-being or health outcomes. Stating how some research is novel is clearer than merely stating that the work is novel. This practice might even help journals and funders identify what type of novelty they would like to reward. In sum, this framework can help identify and articulate how research is novel.

11.8. Help to identify when standardization of pipelines is beneficial or problematic to a field

Many consider standardization in a field to be important for ensuring the comparability of results. Standardization of methods and tools entails that the pipeline P is identical (or at least very similar) across studies. However, in such cases, the standardized pipeline becomes an auxiliary assumption representing all possible pipelines. Therefore, while standardized pipelines have their benefits, this assumption becomes broader without validating (e.g. via multiverse analysis) which pipelines a standardized P represents. In summary, because this framework helps distinguish between auxiliary assumptions and explicit parts of the hypothesis and identifies when a multiverse analysis is needed, it can help determine when standardizations of pipelines are representative (narrower hypotheses) or assumptive (broader hypotheses).

12.  Conclusion

Here, we have argued that the scope of a hypothesis is made up of three dimensions: the relationship ( R ), variable ( XY ) and pipeline ( P ) selection. Along each of these dimensions, the scope can vary. Different types of scientific enterprises will often have hypotheses that vary the size of the scopes. We have argued that this focus on the scope of the hypothesis along these dimensions helps the hypothesis-maker formulate their hypotheses for preregistrations while also helping demarcate auxiliary hypotheses (assumed to be true) from the hypothesis (those being evaluated during the scientific process).

Hypotheses are an essential part of the scientific process. Considering what type of hypothesis is sufficient or relevant is an essential job of the researcher that we think has been overlooked. We hope this work promotes an understanding of what a hypothesis is and how its formulation and reduction in scope is an integral part of scientific progress. We hope it also helps clarify how broad hypotheses need not be vague or inappropriate.

Finally, we applied this idea of scopes to scientific progress and considered how to formulate an appropriate hypothesis. We have also listed several ways researchers can practically implement this framework today. However, there are other practicalities of this framework that future work should explore. For example, it could be used to differentiate and demarcate different scientific contributions (e.g. confirmatory studies, exploration studies, validation studies) with how their hypotheses interact with the different dimensions of the hypothesis space. Further, linking hypotheses over time within this framework can be a foundation for open hypothesis-making by promoting explicit links to previous work and detailing the reduction of the hypothesis space. This framework helps quantify the contribution to the hypothesis space of different studies and helps clarify what aspects of hypotheses can be relevant at different times.

Acknowledgements

We thank Filip Gedin, Kristoffer Sundberg, Jens Fust, and James Steele for valuable feedback on earlier versions of this article. We also thank Mark Rubin and an unnamed reviewer for valuable comments that have improved the article.

1 While this is our intention, we cannot claim that every theory has been accommodated.

2 Similar requirements of science being able to evaluate the hypothesis can be found in pragmatism [ 22 ], logical positivism [ 23 ] and falsification [ 24 ].

3 Although when making inferences about a failed evaluation of a scientific hypothesis it is possible, due to underdetermination, to reject the auxiliary hypothesis instead of rejecting the hypothesis. However, that rejection occurs at a later inference stage. The evaluation using the scientific method aims to test the scientific hypothesis, not the auxiliary assumptions.

4 Although some have argued that this practice is not as problematic or questionable (see [ 34 , 35 ]).

5 Alternatively, theories sometimes expand their boundary conditions. A theory that was previously about ‘humans' can be used with a more inclusive boundary condition. Thus it is possible for the hypothesis-maker to use a theory about humans (decision making) and expand it to fruit flies or plants (see [ 43 ]).

6 A similarity exists here with Popper, where he uses set theory in a similar way to compare theories (not hypotheses). Popper also discusses how theories with overlapping sets but neither is a subset are also comparable (see [ 24 , §§32–34]). We do not exclude this possibility but can require additional assumptions.

7 When this could be unclear, we place the element within quotation marks.

8 Here, we have assumed that there is no interaction between these variables in variable selection. If an interaction between x 1 and x 2 is hypothesized, this should be viewed as a different variable compared to ‘ x 1 or x 2 ’. The motivation behind this is because the hypothesis ‘ x 1 or x 2 ’ is not a superset of the interaction (i.e. ‘ x 1 or x 2 ’ is not necessarily true when the interaction is true). The interaction should, in this case, be considered a third variable (e.g. I( x 1 , x 2 )) and the hypothesis ‘ x 1 or x 2 or I( x 1 , x 2 )’ is broader than ‘ x 1 or x 2 ’.

9 Or possibly ambiguous or inconclusive.

10 This formulation of scope is compatible with different frameworks from the philosophy of science. For example, by narrowing the scope would in a Popperian terminology mean prohibiting more basic statements (thus a narrower hypothesis has a higher degree of falsifiability). The reduction of scope in the relational dimension would in Popperian terminology mean increase in precision (e.g. a circle is more precise than an ellipse since circles are a subset of possible ellipses), whereas reduction in variable selection and pipeline dimension would mean increase universality (e.g. ‘all heavenly bodies' is more universal than just ‘planets') [ 24 ]. For Meehl the reduction of the relationship dimension would amount to decreasing the relative tolerance of a theory to the Spielraum [ 46 ] .

11 If there is no relationship between x and y , we do not need to test if there is a positive relationship. If we know there is a positive relationship between x and y , we do not need to test if there is a relationship. If we know there is a relationship but there is not a positive relationship, then it is possible that they have a negative relationship.

Data accessibility

Declaration of ai use.

We have not used AI-assisted technologies in creating this article.

Authors' contributions

W.H.T.: conceptualization, investigation, writing—original draft, writing—review and editing; S.S.: investigation, writing—original draft, writing—review and editing.

Both authors gave final approval for publication and agreed to be held accountable for the work performed therein.

Conflict of interest declaration

We declare we have no competing interests.

We received no funding for this study.

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9.1: Null and Alternative Hypotheses

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The actual test begins by considering two hypotheses . They are called the null hypothesis and the alternative hypothesis . These hypotheses contain opposing viewpoints.

\(H_0\): The null hypothesis: It is a statement of no difference between the variables—they are not related. This can often be considered the status quo and as a result if you cannot accept the null it requires some action.

\(H_a\): The alternative hypothesis: It is a claim about the population that is contradictory to \(H_0\) and what we conclude when we reject \(H_0\). This is usually what the researcher is trying to prove.

Since the null and alternative hypotheses are contradictory, you must examine evidence to decide if you have enough evidence to reject the null hypothesis or not. The evidence is in the form of sample data.

After you have determined which hypothesis the sample supports, you make a decision. There are two options for a decision. They are "reject \(H_0\)" if the sample information favors the alternative hypothesis or "do not reject \(H_0\)" or "decline to reject \(H_0\)" if the sample information is insufficient to reject the null hypothesis.

\(H_{0}\) always has a symbol with an equal in it. \(H_{a}\) never has a symbol with an equal in it. The choice of symbol depends on the wording of the hypothesis test. However, be aware that many researchers (including one of the co-authors in research work) use = in the null hypothesis, even with > or < as the symbol in the alternative hypothesis. This practice is acceptable because we only make the decision to reject or not reject the null hypothesis.

Example \(\PageIndex{1}\)

  • \(H_{0}\): No more than 30% of the registered voters in Santa Clara County voted in the primary election. \(p \leq 30\)
  • \(H_{a}\): More than 30% of the registered voters in Santa Clara County voted in the primary election. \(p > 30\)

Exercise \(\PageIndex{1}\)

A medical trial is conducted to test whether or not a new medicine reduces cholesterol by 25%. State the null and alternative hypotheses.

  • \(H_{0}\): The drug reduces cholesterol by 25%. \(p = 0.25\)
  • \(H_{a}\): The drug does not reduce cholesterol by 25%. \(p \neq 0.25\)

Example \(\PageIndex{2}\)

We want to test whether the mean GPA of students in American colleges is different from 2.0 (out of 4.0). The null and alternative hypotheses are:

  • \(H_{0}: \mu = 2.0\)
  • \(H_{a}: \mu \neq 2.0\)

Exercise \(\PageIndex{2}\)

We want to test whether the mean height of eighth graders is 66 inches. State the null and alternative hypotheses. Fill in the correct symbol \((=, \neq, \geq, <, \leq, >)\) for the null and alternative hypotheses.

  • \(H_{0}: \mu \_ 66\)
  • \(H_{a}: \mu \_ 66\)
  • \(H_{0}: \mu = 66\)
  • \(H_{a}: \mu \neq 66\)

Example \(\PageIndex{3}\)

We want to test if college students take less than five years to graduate from college, on the average. The null and alternative hypotheses are:

  • \(H_{0}: \mu \geq 5\)
  • \(H_{a}: \mu < 5\)

Exercise \(\PageIndex{3}\)

We want to test if it takes fewer than 45 minutes to teach a lesson plan. State the null and alternative hypotheses. Fill in the correct symbol ( =, ≠, ≥, <, ≤, >) for the null and alternative hypotheses.

  • \(H_{0}: \mu \_ 45\)
  • \(H_{a}: \mu \_ 45\)
  • \(H_{0}: \mu \geq 45\)
  • \(H_{a}: \mu < 45\)

Example \(\PageIndex{4}\)

In an issue of U. S. News and World Report , an article on school standards stated that about half of all students in France, Germany, and Israel take advanced placement exams and a third pass. The same article stated that 6.6% of U.S. students take advanced placement exams and 4.4% pass. Test if the percentage of U.S. students who take advanced placement exams is more than 6.6%. State the null and alternative hypotheses.

  • \(H_{0}: p \leq 0.066\)
  • \(H_{a}: p > 0.066\)

Exercise \(\PageIndex{4}\)

On a state driver’s test, about 40% pass the test on the first try. We want to test if more than 40% pass on the first try. Fill in the correct symbol (\(=, \neq, \geq, <, \leq, >\)) for the null and alternative hypotheses.

  • \(H_{0}: p \_ 0.40\)
  • \(H_{a}: p \_ 0.40\)
  • \(H_{0}: p = 0.40\)
  • \(H_{a}: p > 0.40\)

COLLABORATIVE EXERCISE

Bring to class a newspaper, some news magazines, and some Internet articles . In groups, find articles from which your group can write null and alternative hypotheses. Discuss your hypotheses with the rest of the class.

In a hypothesis test , sample data is evaluated in order to arrive at a decision about some type of claim. If certain conditions about the sample are satisfied, then the claim can be evaluated for a population. In a hypothesis test, we:

  • Evaluate the null hypothesis , typically denoted with \(H_{0}\). The null is not rejected unless the hypothesis test shows otherwise. The null statement must always contain some form of equality \((=, \leq \text{or} \geq)\)
  • Always write the alternative hypothesis , typically denoted with \(H_{a}\) or \(H_{1}\), using less than, greater than, or not equals symbols, i.e., \((\neq, >, \text{or} <)\).
  • If we reject the null hypothesis, then we can assume there is enough evidence to support the alternative hypothesis.
  • Never state that a claim is proven true or false. Keep in mind the underlying fact that hypothesis testing is based on probability laws; therefore, we can talk only in terms of non-absolute certainties.

Formula Review

\(H_{0}\) and \(H_{a}\) are contradictory.

  • If \(\alpha \leq p\)-value, then do not reject \(H_{0}\).
  • If\(\alpha > p\)-value, then reject \(H_{0}\).

\(\alpha\) is preconceived. Its value is set before the hypothesis test starts. The \(p\)-value is calculated from the data.References

Data from the National Institute of Mental Health. Available online at http://www.nimh.nih.gov/publicat/depression.cfm .

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Sat / act prep online guides and tips, what is a hypothesis and how do i write one.

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General Education

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Think about something strange and unexplainable in your life. Maybe you get a headache right before it rains, or maybe you think your favorite sports team wins when you wear a certain color. If you wanted to see whether these are just coincidences or scientific fact, you would form a hypothesis, then create an experiment to see whether that hypothesis is true or not.

But what is a hypothesis, anyway? If you’re not sure about what a hypothesis is--or how to test for one!--you’re in the right place. This article will teach you everything you need to know about hypotheses, including: 

  • Defining the term “hypothesis” 
  • Providing hypothesis examples 
  • Giving you tips for how to write your own hypothesis

So let’s get started!

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What Is a Hypothesis?

Merriam Webster defines a hypothesis as “an assumption or concession made for the sake of argument.” In other words, a hypothesis is an educated guess . Scientists make a reasonable assumption--or a hypothesis--then design an experiment to test whether it’s true or not. Keep in mind that in science, a hypothesis should be testable. You have to be able to design an experiment that tests your hypothesis in order for it to be valid. 

As you could assume from that statement, it’s easy to make a bad hypothesis. But when you’re holding an experiment, it’s even more important that your guesses be good...after all, you’re spending time (and maybe money!) to figure out more about your observation. That’s why we refer to a hypothesis as an educated guess--good hypotheses are based on existing data and research to make them as sound as possible.

Hypotheses are one part of what’s called the scientific method .  Every (good) experiment or study is based in the scientific method. The scientific method gives order and structure to experiments and ensures that interference from scientists or outside influences does not skew the results. It’s important that you understand the concepts of the scientific method before holding your own experiment. Though it may vary among scientists, the scientific method is generally made up of six steps (in order):

  • Observation
  • Asking questions
  • Forming a hypothesis
  • Analyze the data
  • Communicate your results

You’ll notice that the hypothesis comes pretty early on when conducting an experiment. That’s because experiments work best when they’re trying to answer one specific question. And you can’t conduct an experiment until you know what you’re trying to prove!

Independent and Dependent Variables 

After doing your research, you’re ready for another important step in forming your hypothesis: identifying variables. Variables are basically any factor that could influence the outcome of your experiment . Variables have to be measurable and related to the topic being studied.

There are two types of variables:  independent variables and dependent variables. I ndependent variables remain constant . For example, age is an independent variable; it will stay the same, and researchers can look at different ages to see if it has an effect on the dependent variable. 

Speaking of dependent variables... dependent variables are subject to the influence of the independent variable , meaning that they are not constant. Let’s say you want to test whether a person’s age affects how much sleep they need. In that case, the independent variable is age (like we mentioned above), and the dependent variable is how much sleep a person gets. 

Variables will be crucial in writing your hypothesis. You need to be able to identify which variable is which, as both the independent and dependent variables will be written into your hypothesis. For instance, in a study about exercise, the independent variable might be the speed at which the respondents walk for thirty minutes, and the dependent variable would be their heart rate. In your study and in your hypothesis, you’re trying to understand the relationship between the two variables.

Elements of a Good Hypothesis

The best hypotheses start by asking the right questions . For instance, if you’ve observed that the grass is greener when it rains twice a week, you could ask what kind of grass it is, what elevation it’s at, and if the grass across the street responds to rain in the same way. Any of these questions could become the backbone of experiments to test why the grass gets greener when it rains fairly frequently.

As you’re asking more questions about your first observation, make sure you’re also making more observations . If it doesn’t rain for two weeks and the grass still looks green, that’s an important observation that could influence your hypothesis. You'll continue observing all throughout your experiment, but until the hypothesis is finalized, every observation should be noted.

Finally, you should consult secondary research before writing your hypothesis . Secondary research is comprised of results found and published by other people. You can usually find this information online or at your library. Additionally, m ake sure the research you find is credible and related to your topic. If you’re studying the correlation between rain and grass growth, it would help you to research rain patterns over the past twenty years for your county, published by a local agricultural association. You should also research the types of grass common in your area, the type of grass in your lawn, and whether anyone else has conducted experiments about your hypothesis. Also be sure you’re checking the quality of your research . Research done by a middle school student about what minerals can be found in rainwater would be less useful than an article published by a local university.

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Writing Your Hypothesis

Once you’ve considered all of the factors above, you’re ready to start writing your hypothesis. Hypotheses usually take a certain form when they’re written out in a research report.

When you boil down your hypothesis statement, you are writing down your best guess and not the question at hand . This means that your statement should be written as if it is fact already, even though you are simply testing it.

The reason for this is that, after you have completed your study, you'll either accept or reject your if-then or your null hypothesis. All hypothesis testing examples should be measurable and able to be confirmed or denied. You cannot confirm a question, only a statement! 

In fact, you come up with hypothesis examples all the time! For instance, when you guess on the outcome of a basketball game, you don’t say, “Will the Miami Heat beat the Boston Celtics?” but instead, “I think the Miami Heat will beat the Boston Celtics.” You state it as if it is already true, even if it turns out you’re wrong. You do the same thing when writing your hypothesis.

Additionally, keep in mind that hypotheses can range from very specific to very broad.  These hypotheses can be specific, but if your hypothesis testing examples involve a broad range of causes and effects, your hypothesis can also be broad.  

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The Two Types of Hypotheses

Now that you understand what goes into a hypothesis, it’s time to look more closely at the two most common types of hypothesis: the if-then hypothesis and the null hypothesis.

#1: If-Then Hypotheses

First of all, if-then hypotheses typically follow this formula:

If ____ happens, then ____ will happen.

The goal of this type of hypothesis is to test the causal relationship between the independent and dependent variable. It’s fairly simple, and each hypothesis can vary in how detailed it can be. We create if-then hypotheses all the time with our daily predictions. Here are some examples of hypotheses that use an if-then structure from daily life: 

  • If I get enough sleep, I’ll be able to get more work done tomorrow.
  • If the bus is on time, I can make it to my friend’s birthday party. 
  • If I study every night this week, I’ll get a better grade on my exam. 

In each of these situations, you’re making a guess on how an independent variable (sleep, time, or studying) will affect a dependent variable (the amount of work you can do, making it to a party on time, or getting better grades). 

You may still be asking, “What is an example of a hypothesis used in scientific research?” Take one of the hypothesis examples from a real-world study on whether using technology before bed affects children’s sleep patterns. The hypothesis read s:

“We hypothesized that increased hours of tablet- and phone-based screen time at bedtime would be inversely correlated with sleep quality and child attention.”

It might not look like it, but this is an if-then statement. The researchers basically said, “If children have more screen usage at bedtime, then their quality of sleep and attention will be worse.” The sleep quality and attention are the dependent variables and the screen usage is the independent variable. (Usually, the independent variable comes after the “if” and the dependent variable comes after the “then,” as it is the independent variable that affects the dependent variable.) This is an excellent example of how flexible hypothesis statements can be, as long as the general idea of “if-then” and the independent and dependent variables are present.

#2: Null Hypotheses

Your if-then hypothesis is not the only one needed to complete a successful experiment, however. You also need a null hypothesis to test it against. In its most basic form, the null hypothesis is the opposite of your if-then hypothesis . When you write your null hypothesis, you are writing a hypothesis that suggests that your guess is not true, and that the independent and dependent variables have no relationship .

One null hypothesis for the cell phone and sleep study from the last section might say: 

“If children have more screen usage at bedtime, their quality of sleep and attention will not be worse.” 

In this case, this is a null hypothesis because it’s asking the opposite of the original thesis! 

Conversely, if your if-then hypothesis suggests that your two variables have no relationship, then your null hypothesis would suggest that there is one. So, pretend that there is a study that is asking the question, “Does the amount of followers on Instagram influence how long people spend on the app?” The independent variable is the amount of followers, and the dependent variable is the time spent. But if you, as the researcher, don’t think there is a relationship between the number of followers and time spent, you might write an if-then hypothesis that reads:

“If people have many followers on Instagram, they will not spend more time on the app than people who have less.”

In this case, the if-then suggests there isn’t a relationship between the variables. In that case, one of the null hypothesis examples might say:

“If people have many followers on Instagram, they will spend more time on the app than people who have less.”

You then test both the if-then and the null hypothesis to gauge if there is a relationship between the variables, and if so, how much of a relationship. 

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4 Tips to Write the Best Hypothesis

If you’re going to take the time to hold an experiment, whether in school or by yourself, you’re also going to want to take the time to make sure your hypothesis is a good one. The best hypotheses have four major elements in common: plausibility, defined concepts, observability, and general explanation.

#1: Plausibility

At first glance, this quality of a hypothesis might seem obvious. When your hypothesis is plausible, that means it’s possible given what we know about science and general common sense. However, improbable hypotheses are more common than you might think. 

Imagine you’re studying weight gain and television watching habits. If you hypothesize that people who watch more than  twenty hours of television a week will gain two hundred pounds or more over the course of a year, this might be improbable (though it’s potentially possible). Consequently, c ommon sense can tell us the results of the study before the study even begins.

Improbable hypotheses generally go against  science, as well. Take this hypothesis example: 

“If a person smokes one cigarette a day, then they will have lungs just as healthy as the average person’s.” 

This hypothesis is obviously untrue, as studies have shown again and again that cigarettes negatively affect lung health. You must be careful that your hypotheses do not reflect your own personal opinion more than they do scientifically-supported findings. This plausibility points to the necessity of research before the hypothesis is written to make sure that your hypothesis has not already been disproven.

#2: Defined Concepts

The more advanced you are in your studies, the more likely that the terms you’re using in your hypothesis are specific to a limited set of knowledge. One of the hypothesis testing examples might include the readability of printed text in newspapers, where you might use words like “kerning” and “x-height.” Unless your readers have a background in graphic design, it’s likely that they won’t know what you mean by these terms. Thus, it’s important to either write what they mean in the hypothesis itself or in the report before the hypothesis.

Here’s what we mean. Which of the following sentences makes more sense to the common person?

If the kerning is greater than average, more words will be read per minute.

If the space between letters is greater than average, more words will be read per minute.

For people reading your report that are not experts in typography, simply adding a few more words will be helpful in clarifying exactly what the experiment is all about. It’s always a good idea to make your research and findings as accessible as possible. 

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Good hypotheses ensure that you can observe the results. 

#3: Observability

In order to measure the truth or falsity of your hypothesis, you must be able to see your variables and the way they interact. For instance, if your hypothesis is that the flight patterns of satellites affect the strength of certain television signals, yet you don’t have a telescope to view the satellites or a television to monitor the signal strength, you cannot properly observe your hypothesis and thus cannot continue your study.

Some variables may seem easy to observe, but if you do not have a system of measurement in place, you cannot observe your hypothesis properly. Here’s an example: if you’re experimenting on the effect of healthy food on overall happiness, but you don’t have a way to monitor and measure what “overall happiness” means, your results will not reflect the truth. Monitoring how often someone smiles for a whole day is not reasonably observable, but having the participants state how happy they feel on a scale of one to ten is more observable. 

In writing your hypothesis, always keep in mind how you'll execute the experiment.

#4: Generalizability 

Perhaps you’d like to study what color your best friend wears the most often by observing and documenting the colors she wears each day of the week. This might be fun information for her and you to know, but beyond you two, there aren’t many people who could benefit from this experiment. When you start an experiment, you should note how generalizable your findings may be if they are confirmed. Generalizability is basically how common a particular phenomenon is to other people’s everyday life.

Let’s say you’re asking a question about the health benefits of eating an apple for one day only, you need to realize that the experiment may be too specific to be helpful. It does not help to explain a phenomenon that many people experience. If you find yourself with too specific of a hypothesis, go back to asking the big question: what is it that you want to know, and what do you think will happen between your two variables?

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Hypothesis Testing Examples

We know it can be hard to write a good hypothesis unless you’ve seen some good hypothesis examples. We’ve included four hypothesis examples based on some made-up experiments. Use these as templates or launch pads for coming up with your own hypotheses.

Experiment #1: Students Studying Outside (Writing a Hypothesis)

You are a student at PrepScholar University. When you walk around campus, you notice that, when the temperature is above 60 degrees, more students study in the quad. You want to know when your fellow students are more likely to study outside. With this information, how do you make the best hypothesis possible?

You must remember to make additional observations and do secondary research before writing your hypothesis. In doing so, you notice that no one studies outside when it’s 75 degrees and raining, so this should be included in your experiment. Also, studies done on the topic beforehand suggested that students are more likely to study in temperatures less than 85 degrees. With this in mind, you feel confident that you can identify your variables and write your hypotheses:

If-then: “If the temperature in Fahrenheit is less than 60 degrees, significantly fewer students will study outside.”

Null: “If the temperature in Fahrenheit is less than 60 degrees, the same number of students will study outside as when it is more than 60 degrees.”

These hypotheses are plausible, as the temperatures are reasonably within the bounds of what is possible. The number of people in the quad is also easily observable. It is also not a phenomenon specific to only one person or at one time, but instead can explain a phenomenon for a broader group of people.

To complete this experiment, you pick the month of October to observe the quad. Every day (except on the days where it’s raining)from 3 to 4 PM, when most classes have released for the day, you observe how many people are on the quad. You measure how many people come  and how many leave. You also write down the temperature on the hour. 

After writing down all of your observations and putting them on a graph, you find that the most students study on the quad when it is 70 degrees outside, and that the number of students drops a lot once the temperature reaches 60 degrees or below. In this case, your research report would state that you accept or “failed to reject” your first hypothesis with your findings.

Experiment #2: The Cupcake Store (Forming a Simple Experiment)

Let’s say that you work at a bakery. You specialize in cupcakes, and you make only two colors of frosting: yellow and purple. You want to know what kind of customers are more likely to buy what kind of cupcake, so you set up an experiment. Your independent variable is the customer’s gender, and the dependent variable is the color of the frosting. What is an example of a hypothesis that might answer the question of this study?

Here’s what your hypotheses might look like: 

If-then: “If customers’ gender is female, then they will buy more yellow cupcakes than purple cupcakes.”

Null: “If customers’ gender is female, then they will be just as likely to buy purple cupcakes as yellow cupcakes.”

This is a pretty simple experiment! It passes the test of plausibility (there could easily be a difference), defined concepts (there’s nothing complicated about cupcakes!), observability (both color and gender can be easily observed), and general explanation ( this would potentially help you make better business decisions ).

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Experiment #3: Backyard Bird Feeders (Integrating Multiple Variables and Rejecting the If-Then Hypothesis)

While watching your backyard bird feeder, you realized that different birds come on the days when you change the types of seeds. You decide that you want to see more cardinals in your backyard, so you decide to see what type of food they like the best and set up an experiment. 

However, one morning, you notice that, while some cardinals are present, blue jays are eating out of your backyard feeder filled with millet. You decide that, of all of the other birds, you would like to see the blue jays the least. This means you'll have more than one variable in your hypothesis. Your new hypotheses might look like this: 

If-then: “If sunflower seeds are placed in the bird feeders, then more cardinals will come than blue jays. If millet is placed in the bird feeders, then more blue jays will come than cardinals.”

Null: “If either sunflower seeds or millet are placed in the bird, equal numbers of cardinals and blue jays will come.”

Through simple observation, you actually find that cardinals come as often as blue jays when sunflower seeds or millet is in the bird feeder. In this case, you would reject your “if-then” hypothesis and “fail to reject” your null hypothesis . You cannot accept your first hypothesis, because it’s clearly not true. Instead you found that there was actually no relation between your different variables. Consequently, you would need to run more experiments with different variables to see if the new variables impact the results.

Experiment #4: In-Class Survey (Including an Alternative Hypothesis)

You’re about to give a speech in one of your classes about the importance of paying attention. You want to take this opportunity to test a hypothesis you’ve had for a while: 

If-then: If students sit in the first two rows of the classroom, then they will listen better than students who do not.

Null: If students sit in the first two rows of the classroom, then they will not listen better or worse than students who do not.

You give your speech and then ask your teacher if you can hand out a short survey to the class. On the survey, you’ve included questions about some of the topics you talked about. When you get back the results, you’re surprised to see that not only do the students in the first two rows not pay better attention, but they also scored worse than students in other parts of the classroom! Here, both your if-then and your null hypotheses are not representative of your findings. What do you do?

This is when you reject both your if-then and null hypotheses and instead create an alternative hypothesis . This type of hypothesis is used in the rare circumstance that neither of your hypotheses is able to capture your findings . Now you can use what you’ve learned to draft new hypotheses and test again! 

Key Takeaways: Hypothesis Writing

The more comfortable you become with writing hypotheses, the better they will become. The structure of hypotheses is flexible and may need to be changed depending on what topic you are studying. The most important thing to remember is the purpose of your hypothesis and the difference between the if-then and the null . From there, in forming your hypothesis, you should constantly be asking questions, making observations, doing secondary research, and considering your variables. After you have written your hypothesis, be sure to edit it so that it is plausible, clearly defined, observable, and helpful in explaining a general phenomenon.

Writing a hypothesis is something that everyone, from elementary school children competing in a science fair to professional scientists in a lab, needs to know how to do. Hypotheses are vital in experiments and in properly executing the scientific method . When done correctly, hypotheses will set up your studies for success and help you to understand the world a little better, one experiment at a time.

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What’s Next?

If you’re studying for the science portion of the ACT, there’s definitely a lot you need to know. We’ve got the tools to help, though! Start by checking out our ultimate study guide for the ACT Science subject test. Once you read through that, be sure to download our recommended ACT Science practice tests , since they’re one of the most foolproof ways to improve your score. (And don’t forget to check out our expert guide book , too.)

If you love science and want to major in a scientific field, you should start preparing in high school . Here are the science classes you should take to set yourself up for success.

If you’re trying to think of science experiments you can do for class (or for a science fair!), here’s a list of 37 awesome science experiments you can do at home

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Ashley Sufflé Robinson has a Ph.D. in 19th Century English Literature. As a content writer for PrepScholar, Ashley is passionate about giving college-bound students the in-depth information they need to get into the school of their dreams.

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Module 1: Introduction to Biology

Experiments and hypotheses, learning outcomes.

  • Form a hypothesis and use it to design a scientific experiment

Now we’ll focus on the methods of scientific inquiry. Science often involves making observations and developing hypotheses. Experiments and further observations are often used to test the hypotheses.

A scientific experiment is a carefully organized procedure in which the scientist intervenes in a system to change something, then observes the result of the change. Scientific inquiry often involves doing experiments, though not always. For example, a scientist studying the mating behaviors of ladybugs might begin with detailed observations of ladybugs mating in their natural habitats. While this research may not be experimental, it is scientific: it involves careful and verifiable observation of the natural world. The same scientist might then treat some of the ladybugs with a hormone hypothesized to trigger mating and observe whether these ladybugs mated sooner or more often than untreated ones. This would qualify as an experiment because the scientist is now making a change in the system and observing the effects.

Forming a Hypothesis

When conducting scientific experiments, researchers develop hypotheses to guide experimental design. A hypothesis is a suggested explanation that is both testable and falsifiable. You must be able to test your hypothesis, and it must be possible to prove your hypothesis true or false.

For example, Michael observes that maple trees lose their leaves in the fall. He might then propose a possible explanation for this observation: “cold weather causes maple trees to lose their leaves in the fall.” This statement is testable. He could grow maple trees in a warm enclosed environment such as a greenhouse and see if their leaves still dropped in the fall. The hypothesis is also falsifiable. If the leaves still dropped in the warm environment, then clearly temperature was not the main factor in causing maple leaves to drop in autumn.

In the Try It below, you can practice recognizing scientific hypotheses. As you consider each statement, try to think as a scientist would: can I test this hypothesis with observations or experiments? Is the statement falsifiable? If the answer to either of these questions is “no,” the statement is not a valid scientific hypothesis.

Practice Questions

Determine whether each following statement is a scientific hypothesis.

Air pollution from automobile exhaust can trigger symptoms in people with asthma.

  • No. This statement is not testable or falsifiable.
  • No. This statement is not testable.
  • No. This statement is not falsifiable.
  • Yes. This statement is testable and falsifiable.

Natural disasters, such as tornadoes, are punishments for bad thoughts and behaviors.

a: No. This statement is not testable or falsifiable. “Bad thoughts and behaviors” are excessively vague and subjective variables that would be impossible to measure or agree upon in a reliable way. The statement might be “falsifiable” if you came up with a counterexample: a “wicked” place that was not punished by a natural disaster. But some would question whether the people in that place were really wicked, and others would continue to predict that a natural disaster was bound to strike that place at some point. There is no reason to suspect that people’s immoral behavior affects the weather unless you bring up the intervention of a supernatural being, making this idea even harder to test.

Testing a Vaccine

Let’s examine the scientific process by discussing an actual scientific experiment conducted by researchers at the University of Washington. These researchers investigated whether a vaccine may reduce the incidence of the human papillomavirus (HPV). The experimental process and results were published in an article titled, “ A controlled trial of a human papillomavirus type 16 vaccine .”

Preliminary observations made by the researchers who conducted the HPV experiment are listed below:

  • Human papillomavirus (HPV) is the most common sexually transmitted virus in the United States.
  • There are about 40 different types of HPV. A significant number of people that have HPV are unaware of it because many of these viruses cause no symptoms.
  • Some types of HPV can cause cervical cancer.
  • About 4,000 women a year die of cervical cancer in the United States.

Practice Question

Researchers have developed a potential vaccine against HPV and want to test it. What is the first testable hypothesis that the researchers should study?

  • HPV causes cervical cancer.
  • People should not have unprotected sex with many partners.
  • People who get the vaccine will not get HPV.
  • The HPV vaccine will protect people against cancer.

Experimental Design

You’ve successfully identified a hypothesis for the University of Washington’s study on HPV: People who get the HPV vaccine will not get HPV.

The next step is to design an experiment that will test this hypothesis. There are several important factors to consider when designing a scientific experiment. First, scientific experiments must have an experimental group. This is the group that receives the experimental treatment necessary to address the hypothesis.

The experimental group receives the vaccine, but how can we know if the vaccine made a difference? Many things may change HPV infection rates in a group of people over time. To clearly show that the vaccine was effective in helping the experimental group, we need to include in our study an otherwise similar control group that does not get the treatment. We can then compare the two groups and determine if the vaccine made a difference. The control group shows us what happens in the absence of the factor under study.

However, the control group cannot get “nothing.” Instead, the control group often receives a placebo. A placebo is a procedure that has no expected therapeutic effect—such as giving a person a sugar pill or a shot containing only plain saline solution with no drug. Scientific studies have shown that the “placebo effect” can alter experimental results because when individuals are told that they are or are not being treated, this knowledge can alter their actions or their emotions, which can then alter the results of the experiment.

Moreover, if the doctor knows which group a patient is in, this can also influence the results of the experiment. Without saying so directly, the doctor may show—through body language or other subtle cues—their views about whether the patient is likely to get well. These errors can then alter the patient’s experience and change the results of the experiment. Therefore, many clinical studies are “double blind.” In these studies, neither the doctor nor the patient knows which group the patient is in until all experimental results have been collected.

Both placebo treatments and double-blind procedures are designed to prevent bias. Bias is any systematic error that makes a particular experimental outcome more or less likely. Errors can happen in any experiment: people make mistakes in measurement, instruments fail, computer glitches can alter data. But most such errors are random and don’t favor one outcome over another. Patients’ belief in a treatment can make it more likely to appear to “work.” Placebos and double-blind procedures are used to level the playing field so that both groups of study subjects are treated equally and share similar beliefs about their treatment.

The scientists who are researching the effectiveness of the HPV vaccine will test their hypothesis by separating 2,392 young women into two groups: the control group and the experimental group. Answer the following questions about these two groups.

  • This group is given a placebo.
  • This group is deliberately infected with HPV.
  • This group is given nothing.
  • This group is given the HPV vaccine.
  • a: This group is given a placebo. A placebo will be a shot, just like the HPV vaccine, but it will have no active ingredient. It may change peoples’ thinking or behavior to have such a shot given to them, but it will not stimulate the immune systems of the subjects in the same way as predicted for the vaccine itself.
  • d: This group is given the HPV vaccine. The experimental group will receive the HPV vaccine and researchers will then be able to see if it works, when compared to the control group.

Experimental Variables

A variable is a characteristic of a subject (in this case, of a person in the study) that can vary over time or among individuals. Sometimes a variable takes the form of a category, such as male or female; often a variable can be measured precisely, such as body height. Ideally, only one variable is different between the control group and the experimental group in a scientific experiment. Otherwise, the researchers will not be able to determine which variable caused any differences seen in the results. For example, imagine that the people in the control group were, on average, much more sexually active than the people in the experimental group. If, at the end of the experiment, the control group had a higher rate of HPV infection, could you confidently determine why? Maybe the experimental subjects were protected by the vaccine, but maybe they were protected by their low level of sexual contact.

To avoid this situation, experimenters make sure that their subject groups are as similar as possible in all variables except for the variable that is being tested in the experiment. This variable, or factor, will be deliberately changed in the experimental group. The one variable that is different between the two groups is called the independent variable. An independent variable is known or hypothesized to cause some outcome. Imagine an educational researcher investigating the effectiveness of a new teaching strategy in a classroom. The experimental group receives the new teaching strategy, while the control group receives the traditional strategy. It is the teaching strategy that is the independent variable in this scenario. In an experiment, the independent variable is the variable that the scientist deliberately changes or imposes on the subjects.

Dependent variables are known or hypothesized consequences; they are the effects that result from changes or differences in an independent variable. In an experiment, the dependent variables are those that the scientist measures before, during, and particularly at the end of the experiment to see if they have changed as expected. The dependent variable must be stated so that it is clear how it will be observed or measured. Rather than comparing “learning” among students (which is a vague and difficult to measure concept), an educational researcher might choose to compare test scores, which are very specific and easy to measure.

In any real-world example, many, many variables MIGHT affect the outcome of an experiment, yet only one or a few independent variables can be tested. Other variables must be kept as similar as possible between the study groups and are called control variables . For our educational research example, if the control group consisted only of people between the ages of 18 and 20 and the experimental group contained people between the ages of 30 and 35, we would not know if it was the teaching strategy or the students’ ages that played a larger role in the results. To avoid this problem, a good study will be set up so that each group contains students with a similar age profile. In a well-designed educational research study, student age will be a controlled variable, along with other possibly important factors like gender, past educational achievement, and pre-existing knowledge of the subject area.

What is the independent variable in this experiment?

  • Sex (all of the subjects will be female)
  • Presence or absence of the HPV vaccine
  • Presence or absence of HPV (the virus)

List three control variables other than age.

What is the dependent variable in this experiment?

  • Sex (male or female)
  • Rates of HPV infection
  • Age (years)
  • Revision and adaptation. Authored by : Shelli Carter and Lumen Learning. Provided by : Lumen Learning. License : CC BY-NC-SA: Attribution-NonCommercial-ShareAlike
  • Scientific Inquiry. Provided by : Open Learning Initiative. Located at : https://oli.cmu.edu/jcourse/workbook/activity/page?context=434a5c2680020ca6017c03488572e0f8 . Project : Introduction to Biology (Open + Free). License : CC BY-NC-SA: Attribution-NonCommercial-ShareAlike

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5 Characteristics of a Good Hypothesis: A Guide for Researchers

  • by Brian Thomas
  • October 10, 2023

Are you a curious soul, always seeking answers to the whys and hows of the world? As a researcher, formulating a hypothesis is a crucial first step towards unraveling the mysteries of your study. A well-crafted hypothesis not only guides your research but also lays the foundation for drawing valid conclusions. But what exactly makes a hypothesis a good one? In this blog post, we will explore the five key characteristics of a good hypothesis that every researcher should know.

Here, we will delve into the world of hypotheses, covering everything from their types in research to understanding if they can be proven true. Whether you’re a seasoned researcher or just starting out, this blog post will provide valuable insights on how to craft a sound hypothesis for your study. So let’s dive in and uncover the secrets to formulating a hypothesis that stands strong amidst the scientific rigor!

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5 Characteristics of a Good Hypothesis

Clear and specific.

A good hypothesis is like a GPS that guides you to the right destination. It needs to be clear and specific so that you know exactly what you’re testing. Avoid vague statements or general ideas. Instead, focus on crafting a hypothesis that clearly states the relationship between variables and the expected outcome. Clarity is key, my friend!

Testable and Falsifiable

A hypothesis might sound great in theory, but if you can’t test it or prove it wrong, then it’s like chasing unicorns. A good hypothesis should be testable and falsifiable – meaning there should be a way to gather evidence to support or refute it. Don’t be afraid to challenge your hypothesis and put it to the test. Only when it can be proven false can it truly be considered a good hypothesis.

Based on Existing Knowledge

Imagine trying to build a Lego tower without any Lego bricks. That’s what it’s like to come up with a hypothesis that has no basis in existing knowledge. A good hypothesis is grounded in previous research, theories, or observations. It shows that you’ve done your homework and understand the current state of knowledge in your field. So, put on your research hat and gather those building blocks for a solid hypothesis!

Specific Predictions

No, we’re not talking about crystal ball predictions or psychic abilities here. A good hypothesis includes specific predictions about what you expect to happen. It’s like making an educated guess based on your understanding of the variables involved. These predictions help guide your research and give you something concrete to look for. So, put on those prediction goggles, my friend, and let’s get specific!

Relevant to the Research Question

A hypothesis is a road sign that points you in the right direction. But if it’s not relevant to your research question, then you might end up in a never-ending detour. A good hypothesis aligns with your research question and addresses the specific problem or phenomenon you’re investigating. Keep your focus on the main topic and avoid getting sidetracked by shiny distractions. Stay relevant, my friend, and you’ll find the answers you seek!

And there you have it: the five characteristics of a good hypothesis. Remember, a good hypothesis is clear, testable, based on existing knowledge, makes specific predictions, and is relevant to your research question. So go forth, my friend, and hypothesize your way to scientific discovery!

FAQs: Characteristics of a Good Hypothesis

In the realm of scientific research, a hypothesis plays a crucial role in formulating and testing ideas. A good hypothesis serves as the foundation for an experiment or study, guiding the researcher towards meaningful results. In this FAQ-style subsection, we’ll explore the characteristics of a good hypothesis, their types, formulation, and more. So let’s dive in and unravel the mysteries of hypothesis-making!

What Are Two Important Characteristics of a Good Hypothesis

A good hypothesis possesses two important characteristics:

Testability : A hypothesis must be testable to determine its validity. It should be formulated in a way that allows researchers to design and conduct experiments or gather data for analysis. For example, if we hypothesize that “drinking herbal tea reduces stress,” we can easily test it by conducting a study with a control group and a group drinking herbal tea.

Falsifiability : Falsifiability refers to the potential for a hypothesis to be proven wrong. A good hypothesis should make specific predictions that can be refuted or supported by evidence. This characteristic ensures that hypotheses are based on empirical observations rather than personal opinions. For instance, the hypothesis “all swans are white” can be falsified by discovering a single black swan.

What Are the Types of Hypothesis in Research

In research, there are three main types of hypotheses:

Null Hypothesis (H0) : The null hypothesis is a statement of no effect or relationship. It assumes that there is no significant difference between variables or no effect of a treatment. Researchers aim to reject the null hypothesis in favor of an alternative hypothesis.

Alternative Hypothesis (HA or H1) : The alternative hypothesis is the opposite of the null hypothesis. It asserts that there is a significant difference between variables or an effect of a treatment. Researchers seek evidence to support the alternative hypothesis.

Directional Hypothesis : A directional hypothesis predicts the specific direction of the relationship or difference between variables. For example, “increasing exercise duration will lead to greater weight loss.”

Can a Hypothesis Be Proven True

In scientific research, hypotheses are not proven true; they are supported or rejected based on empirical evidence . Even if a hypothesis is supported by multiple studies, new evidence could arise that contradicts it. Scientific knowledge is always subject to revision and refinement. Therefore, the goal is to gather enough evidence to either support or reject a hypothesis, rather than proving it absolutely true.

What Are the Six Parts of a Hypothesis

A hypothesis typically consists of six essential parts:

Research Question : A clear and concise question that the hypothesis seeks to answer.

Variables : Identification of the independent (manipulated) and dependent (measured) variables involved in the hypothesis.

Population : The specific group or individuals the hypothesis is concerned with.

Relationship or Comparison : The expected relationship or difference between variables, often indicated by directional terms like “more,” “less,” “higher,” or “lower.”

Predictability : A statement of the predicted outcome or result based on the relationship between variables.

Testability : The ability to design an experiment or gather data to support or reject the hypothesis.

How Do You Start a Hypothesis Sentence

When starting a hypothesis sentence, it is essential to use clear and concise language to express your ideas. A common approach is to use the phrase “If…then…” to establish the conditional relationship between variables. For example:

  • If [independent variable], then [dependent variable] because [explanation of expected relationship].

This structure allows for a straightforward and logical formulation of the hypothesis.

What Are Examples of Hypotheses

Here are a few examples of well-formulated hypotheses:

If exposure to sunlight increases, then plants will grow taller because sunlight is necessary for photosynthesis.

If students receive praise for good grades, then their motivation to excel will increase because they seek recognition and approval.

If the dose of a painkiller is increased, then the relief from pain will last longer because a higher dosage has a prolonged effect.

What Are the Five Key Elements to a Good Hypothesis

A good hypothesis should include the following five key elements:

Clarity : The hypothesis should be clear and specific, leaving no room for interpretation.

Testability : It should be possible to test the hypothesis through experimentation or data collection.

Relevance : The hypothesis should be directly tied to the research question or problem being investigated.

Specificity : It must clearly state the relationship or difference between variables being studied.

Falsifiability : The hypothesis should make predictions that can be refuted or supported by empirical evidence.

What Makes a Good Hypothesis in a Research Paper

In a research paper, a good hypothesis should have the following characteristics:

Relevance : It must directly relate to the research topic and address the objectives of the study.

Clarity : The hypothesis should be concise and precisely worded to avoid confusion.

Unambiguous : It must leave no room for multiple interpretations or ambiguity.

Logic : The hypothesis should be based on rational and logical reasoning, considering existing theories and observations.

Empirical Support : Ideally, the hypothesis should be supported by prior empirical evidence or strong theoretical justifications.

Is a Hypothesis Always a Question

No, a hypothesis is not always in the form of a question. While some hypotheses can take the form of a question, others may be statements asserting a relationship or difference between variables. The form of a hypothesis depends on the research question being addressed and the researcher’s preferred style of expression.

What Are the Three Things Needed for a Good Hypothesis

For a hypothesis to be considered good, it must fulfill the following three criteria:

Testability : The hypothesis should be formulated in a way that allows for empirical testing through experimentation or data collection.

Falsifiability : It must make specific predictions that can be potentially refuted or supported by evidence.

Relevance : The hypothesis should directly address the research question or problem being investigated.

What Are the Four Components to a Good Hypothesis

A good hypothesis typically consists of four components:

Independent Variable : The variable being manipulated or controlled by the researcher.

Dependent Variable : The variable being measured or observed to determine the effect of the independent variable.

Directionality : The predicted relationship or difference between the independent and dependent variables.

Population : The specific group or individuals to which the hypothesis applies.

How Do You Formulate a Hypothesis

To formulate a hypothesis, follow these steps:

Identify the Research Topic : Clearly define the area or phenomenon you want to study.

Conduct Background Research : Review existing literature and research to gain knowledge about the topic.

Formulate a Research Question : Ask a clear and focused question that you want to answer through your hypothesis.

State the Null and Alternative Hypotheses : Develop a null hypothesis to assume no effect or relationship, and an alternative hypothesis to propose a significant effect or relationship.

Decide on Variables and Relationships : Determine the independent and dependent variables and the predicted relationship between them.

Refine and Test : Refine your hypothesis, ensuring it is clear, testable, and falsifiable. Then, design experiments or gather data to support or reject it.

What Is a Characteristic of a Hypothesis MCQ

Multiple-choice questions (MCQ) regarding the characteristics of a hypothesis often assess knowledge on the testability and falsifiability of hypotheses. They may ask about the criteria that distinguish a good hypothesis from a poor one or the importance of making specific predictions. Remember to choose answers that emphasize the empirical and testable nature of hypotheses.

What Five Criteria Must Be Satisfied for a Hypothesis to Be Scientific

For a hypothesis to be considered scientific, it must satisfy the following five criteria:

Testability : The hypothesis must be formulated in a way that allows it to be tested through experimentation or data collection.

Falsifiability : It should make specific predictions that can be potentially refuted or supported by empirical evidence.

Empirical Basis : The hypothesis should be based on empirical observations or existing theories and knowledge.

Relevance : It must directly address the research question or problem being investigated.

Objective : A scientific hypothesis should be free from personal biases or subjective opinions, focusing on objective observations and analysis.

What Are the Steps of Theory Development in Scientific Methods

In scientific methods, theory development typically involves the following steps:

Observation : Identifying a phenomenon or pattern worthy of investigation through observation or empirical data.

Formulation of a Hypothesis : Constructing a hypothesis that explains the observed phenomena or predicts a relationship between variables.

Data Collection : Gathering relevant data through experiments, surveys, observations, or other research methods.

Analysis : Analyzing the collected data to evaluate the hypothesis’s predictions and determine their validity.

Revision and Refinement : Based on the analysis, refining the hypothesis, modifying the theory, or formulating new hypotheses for further investigation.

Which of the Following Makes a Good Hypothesis

A good hypothesis is characterized by:

Testability : The ability to form experiments or gather data to support or refute the hypothesis.

Falsifiability : The potential for the hypothesis’s predictions to be proven wrong based on empirical evidence.

Clarity : A clear and concise statement or question that leaves no room for ambiguity.

Relevancy : Directly addressing the research question or problem at hand.

Remember, it is important to select the option that encompasses all these characteristics.

What Are the Characteristics of a Good Hypothesis

A good hypothesis possesses several characteristics, such as:

Testability : It should allow for empirical testing through experiments or data collection.

Falsifiability : The hypothesis should make specific predictions that can be potentially refuted or supported by evidence.

Clarity : It must be clearly and precisely formulated, leaving no room for ambiguity or multiple interpretations.

Relevance : The hypothesis should directly relate to the research question or problem being investigated.

What Is the Five-Step p-value Approach to Hypothesis Testing

The five-step p-value approach is a commonly used framework for hypothesis testing:

Step 1: Formulating the Hypotheses : The null hypothesis (H0) assumes no effect or relationship, while the alternative hypothesis (HA) proposes a significant effect or relationship.

Step 2: Setting the Significance Level : Decide on the level of significance (α), which represents the probability of rejecting the null hypothesis when it is true. The commonly used level is 0.05 (5%).

Step 3: Collecting Data and Performing the Test : Acquire and analyze the data, calculating the test statistic and the corresponding p-value.

Step 4: Comparing the p-value with the Significance Level : If the p-value is less than the significance level (α), reject the null hypothesis. Otherwise, fail to reject the null hypothesis.

Step 5: Drawing Conclusions : Based on the comparison in Step 4, interpret the results and draw conclusions about the hypothesis.

What Are the Stages of Hypothesis

The stages of hypothesis generally include:

Observation : Identifying a pattern, phenomenon, or research question that warrants investigation.

Formulation : Developing a hypothesis that explains or predicts the relationship or difference between variables.

Testing : Collecting data, designing experiments, or conducting studies to gather evidence supporting or refuting the hypothesis.

Analysis : Assessing the collected data to determine whether the results support or reject the hypothesis.

Conclusion : Drawing conclusions based on the analysis and making further iterations, refinements, or new hypotheses for future research.

What Is a Characteristic of a Good Hypothesis

A characteristic of a good hypothesis is its ability to make specific predictions about the relationship or difference between variables. Good hypotheses avoid vague statements and clearly articulate the expected outcomes. By doing so, researchers can design experiments or gather data that directly test the predictions, leading to meaningful results.

How Do You Write a Good Hypothesis Example

To write a good hypothesis example, follow these guidelines:

If possible, use the “If…then…” format to express a conditional relationship between variables.

Be clear and concise in stating the variables involved, the predicted relationship, and the expected outcome.

Ensure the hypothesis is testable, meaning it can be evaluated through experiments or data collection.

For instance, consider the following example:

If students study for longer periods of time, then their test scores will improve because increased study time allows for better retention of information and increased proficiency.

What Is the Difference Between Hypothesis and Hypotheses

The main difference between a hypothesis and hypotheses lies in their grammatical number. A hypothesis refers to a single statement or proposition that is formulated to explain or predict the relationship between variables. On the other hand, hypotheses is the plural form of the term hypothesis, commonly used when multiple statements or propositions are proposed and tested simultaneously.

What Is a Good Hypothesis Statement

A good hypothesis statement exhibits the following qualities:

Clarity : It is written in clear and concise language, leaving no room for confusion or ambiguity.

Testability : The hypothesis should be formulated in a way that enables testing through experiments or data collection.

Specificity : It must clearly state the predicted relationship or difference between variables.

By adhering to these criteria, a good hypothesis statement guides research efforts effectively.

What Is Not a Characteristic of a Good Hypothesis

A characteristic that does not align with a good hypothesis is subjectivity . A hypothesis should be objective, based on empirical observations or existing theories, and free from personal bias. While personal interpretations and opinions can inspire the formulation of a hypothesis, it must ultimately rely on objective observations and be open to empirical testing.

By now, you’ve gained insights into the characteristics of a good hypothesis, including testability, falsifiability, clarity,

  • characteristics
  • falsifiable
  • good hypothesis
  • hypothesis testing
  • null hypothesis
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  • scientific rigor

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4.14: Experiments and Hypotheses

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  • Page ID 43806

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Now we’ll focus on the methods of scientific inquiry. Science often involves making observations and developing hypotheses. Experiments and further observations are often used to test the hypotheses.

A scientific experiment is a carefully organized procedure in which the scientist intervenes in a system to change something, then observes the result of the change. Scientific inquiry often involves doing experiments, though not always. For example, a scientist studying the mating behaviors of ladybugs might begin with detailed observations of ladybugs mating in their natural habitats. While this research may not be experimental, it is scientific: it involves careful and verifiable observation of the natural world. The same scientist might then treat some of the ladybugs with a hormone hypothesized to trigger mating and observe whether these ladybugs mated sooner or more often than untreated ones. This would qualify as an experiment because the scientist is now making a change in the system and observing the effects.

Forming a Hypothesis

When conducting scientific experiments, researchers develop hypotheses to guide experimental design. A hypothesis is a suggested explanation that is both testable and falsifiable. You must be able to test your hypothesis, and it must be possible to prove your hypothesis true or false.

For example, Michael observes that maple trees lose their leaves in the fall. He might then propose a possible explanation for this observation: “cold weather causes maple trees to lose their leaves in the fall.” This statement is testable. He could grow maple trees in a warm enclosed environment such as a greenhouse and see if their leaves still dropped in the fall. The hypothesis is also falsifiable. If the leaves still dropped in the warm environment, then clearly temperature was not the main factor in causing maple leaves to drop in autumn.

In the Try It below, you can practice recognizing scientific hypotheses. As you consider each statement, try to think as a scientist would: can I test this hypothesis with observations or experiments? Is the statement falsifiable? If the answer to either of these questions is “no,” the statement is not a valid scientific hypothesis.

Practice Questions

Determine whether each following statement is a scientific hypothesis.

  • No. This statement is not testable or falsifiable.
  • No. This statement is not testable.
  • No. This statement is not falsifiable.
  • Yes. This statement is testable and falsifiable.

[reveal-answer q=”429550″] Show Answers [/reveal-answer] [hidden-answer a=”429550″]

  • d: Yes. This statement is testable and falsifiable. This could be tested with a number of different kinds of observations and experiments, and it is possible to gather evidence that indicates that air pollution is not linked with asthma.
  • a: No. This statement is not testable or falsifiable. “Bad thoughts and behaviors” are excessively vague and subjective variables that would be impossible to measure or agree upon in a reliable way. The statement might be “falsifiable” if you came up with a counterexample: a “wicked” place that was not punished by a natural disaster. But some would question whether the people in that place were really wicked, and others would continue to predict that a natural disaster was bound to strike that place at some point. There is no reason to suspect that people’s immoral behavior affects the weather unless you bring up the intervention of a supernatural being, making this idea even harder to test.

[/hidden-answer]

Testing a Vaccine

Let’s examine the scientific process by discussing an actual scientific experiment conducted by researchers at the University of Washington. These researchers investigated whether a vaccine may reduce the incidence of the human papillomavirus (HPV). The experimental process and results were published in an article titled, “ A controlled trial of a human papillomavirus type 16 vaccine .”

Preliminary observations made by the researchers who conducted the HPV experiment are listed below:

  • Human papillomavirus (HPV) is the most common sexually transmitted virus in the United States.
  • There are about 40 different types of HPV. A significant number of people that have HPV are unaware of it because many of these viruses cause no symptoms.
  • Some types of HPV can cause cervical cancer.
  • About 4,000 women a year die of cervical cancer in the United States.

Practice Question

Researchers have developed a potential vaccine against HPV and want to test it. What is the first testable hypothesis that the researchers should study?

  • HPV causes cervical cancer.
  • People should not have unprotected sex with many partners.
  • People who get the vaccine will not get HPV.
  • The HPV vaccine will protect people against cancer.

[reveal-answer q=”20917″] Show Answer [/reveal-answer] [hidden-answer a=”20917″]Hypothesis A is not the best choice because this information is already known from previous studies. Hypothesis B is not testable because scientific hypotheses are not value statements; they do not include judgments like “should,” “better than,” etc. Scientific evidence certainly might support this value judgment, but a hypothesis would take a different form: “Having unprotected sex with many partners increases a person’s risk for cervical cancer.” Before the researchers can test if the vaccine protects against cancer (hypothesis D), they want to test if it protects against the virus. This statement will make an excellent hypothesis for the next study. The researchers should first test hypothesis C—whether or not the new vaccine can prevent HPV.[/hidden-answer]

Experimental Design

You’ve successfully identified a hypothesis for the University of Washington’s study on HPV: People who get the HPV vaccine will not get HPV.

The next step is to design an experiment that will test this hypothesis. There are several important factors to consider when designing a scientific experiment. First, scientific experiments must have an experimental group. This is the group that receives the experimental treatment necessary to address the hypothesis.

The experimental group receives the vaccine, but how can we know if the vaccine made a difference? Many things may change HPV infection rates in a group of people over time. To clearly show that the vaccine was effective in helping the experimental group, we need to include in our study an otherwise similar control group that does not get the treatment. We can then compare the two groups and determine if the vaccine made a difference. The control group shows us what happens in the absence of the factor under study.

However, the control group cannot get “nothing.” Instead, the control group often receives a placebo. A placebo is a procedure that has no expected therapeutic effect—such as giving a person a sugar pill or a shot containing only plain saline solution with no drug. Scientific studies have shown that the “placebo effect” can alter experimental results because when individuals are told that they are or are not being treated, this knowledge can alter their actions or their emotions, which can then alter the results of the experiment.

Moreover, if the doctor knows which group a patient is in, this can also influence the results of the experiment. Without saying so directly, the doctor may show—through body language or other subtle cues—his or her views about whether the patient is likely to get well. These errors can then alter the patient’s experience and change the results of the experiment. Therefore, many clinical studies are “double blind.” In these studies, neither the doctor nor the patient knows which group the patient is in until all experimental results have been collected.

Both placebo treatments and double-blind procedures are designed to prevent bias. Bias is any systematic error that makes a particular experimental outcome more or less likely. Errors can happen in any experiment: people make mistakes in measurement, instruments fail, computer glitches can alter data. But most such errors are random and don’t favor one outcome over another. Patients’ belief in a treatment can make it more likely to appear to “work.” Placebos and double-blind procedures are used to level the playing field so that both groups of study subjects are treated equally and share similar beliefs about their treatment.

The scientists who are researching the effectiveness of the HPV vaccine will test their hypothesis by separating 2,392 young women into two groups: the control group and the experimental group. Answer the following questions about these two groups.

  • This group is given a placebo.
  • This group is deliberately infected with HPV.
  • This group is given nothing.
  • This group is given the HPV vaccine.

[reveal-answer q=”918962″] Show Answers [/reveal-answer] [hidden-answer a=”918962″]

  • a: This group is given a placebo. A placebo will be a shot, just like the HPV vaccine, but it will have no active ingredient. It may change peoples’ thinking or behavior to have such a shot given to them, but it will not stimulate the immune systems of the subjects in the same way as predicted for the vaccine itself.
  • d: This group is given the HPV vaccine. The experimental group will receive the HPV vaccine and researchers will then be able to see if it works, when compared to the control group.

Experimental Variables

A variable is a characteristic of a subject (in this case, of a person in the study) that can vary over time or among individuals. Sometimes a variable takes the form of a category, such as male or female; often a variable can be measured precisely, such as body height. Ideally, only one variable is different between the control group and the experimental group in a scientific experiment. Otherwise, the researchers will not be able to determine which variable caused any differences seen in the results. For example, imagine that the people in the control group were, on average, much more sexually active than the people in the experimental group. If, at the end of the experiment, the control group had a higher rate of HPV infection, could you confidently determine why? Maybe the experimental subjects were protected by the vaccine, but maybe they were protected by their low level of sexual contact.

To avoid this situation, experimenters make sure that their subject groups are as similar as possible in all variables except for the variable that is being tested in the experiment. This variable, or factor, will be deliberately changed in the experimental group. The one variable that is different between the two groups is called the independent variable. An independent variable is known or hypothesized to cause some outcome. Imagine an educational researcher investigating the effectiveness of a new teaching strategy in a classroom. The experimental group receives the new teaching strategy, while the control group receives the traditional strategy. It is the teaching strategy that is the independent variable in this scenario. In an experiment, the independent variable is the variable that the scientist deliberately changes or imposes on the subjects.

Dependent variables are known or hypothesized consequences; they are the effects that result from changes or differences in an independent variable. In an experiment, the dependent variables are those that the scientist measures before, during, and particularly at the end of the experiment to see if they have changed as expected. The dependent variable must be stated so that it is clear how it will be observed or measured. Rather than comparing “learning” among students (which is a vague and difficult to measure concept), an educational researcher might choose to compare test scores, which are very specific and easy to measure.

In any real-world example, many, many variables MIGHT affect the outcome of an experiment, yet only one or a few independent variables can be tested. Other variables must be kept as similar as possible between the study groups and are called control variables . For our educational research example, if the control group consisted only of people between the ages of 18 and 20 and the experimental group contained people between the ages of 30 and 35, we would not know if it was the teaching strategy or the students’ ages that played a larger role in the results. To avoid this problem, a good study will be set up so that each group contains students with a similar age profile. In a well-designed educational research study, student age will be a controlled variable, along with other possibly important factors like gender, past educational achievement, and pre-existing knowledge of the subject area.

What is the independent variable in this experiment?

  • Sex (all of the subjects will be female)
  • Presence or absence of the HPV vaccine
  • Presence or absence of HPV (the virus)

[reveal-answer q=”68680″]Show Answer[/reveal-answer] [hidden-answer a=”68680″]Answer b. Presence or absence of the HPV vaccine. This is the variable that is different between the control and the experimental groups. All the subjects in this study are female, so this variable is the same in all groups. In a well-designed study, the two groups will be of similar age. The presence or absence of the virus is what the researchers will measure at the end of the experiment. Ideally the two groups will both be HPV-free at the start of the experiment.

List three control variables other than age.

[practice-area rows=”3″][/practice-area] [reveal-answer q=”903121″]Show Answer[/reveal-answer] [hidden-answer a=”903121″]Some possible control variables would be: general health of the women, sexual activity, lifestyle, diet, socioeconomic status, etc.

What is the dependent variable in this experiment?

  • Sex (male or female)
  • Rates of HPV infection
  • Age (years)

[reveal-answer q=”907103″]Show Answer[/reveal-answer] [hidden-answer a=”907103″]Answer b. Rates of HPV infection. The researchers will measure how many individuals got infected with HPV after a given period of time.[/hidden-answer]

Contributors and Attributions

  • Revision and adaptation. Authored by : Shelli Carter and Lumen Learning. Provided by : Lumen Learning. License : CC BY-NC-SA: Attribution-NonCommercial-ShareAlike
  • Scientific Inquiry. Provided by : Open Learning Initiative. Located at : https://oli.cmu.edu/jcourse/workbook/activity/page?context=434a5c2680020ca6017c03488572e0f8 . Project : Introduction to Biology (Open + Free). License : CC BY-NC-SA: Attribution-NonCommercial-ShareAlike

Susan Krauss Whitbourne Ph.D.

  • Personality

14 Ways to Tell if Your Personality Is Working Against You

New research shows how having a certain personality type can be bad for your heart..

Posted May 14, 2024 | Reviewed by Michelle Quirk

  • What Is Personality?
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  • The Type A personality was found to be an invalid concept based on retractions of the original research.
  • Type D personality remains a valid concept, and as shown in new research, can help explain heart health.
  • By tuning into your emotional reactivity, you can keep your stress levels, and your heart, in shape.

When the Type A personality research was revealed to be based on fraudulent research, its retraction caused shock waves to reverberate throughout the behavioral medicine community. After all, doesn’t it make sense that people whose personality leads them to be hard-driving, competitive, and impatient would be heart attacks just waiting to happen? Although they may not be pleasant to be with, these people formerly known as Type A don’t seem to be any worse off than their counterparts, the so-called Type B. What’s more, the Type C personality also proposed by behavioral health researchers is based on just as flawed a set of studies.

The tendency to type people by letters ended in the alphabet with Type D, which remains the only personality style standing amidst all the retractions and controversy. According to a new systematic review of the literature, it still appears to hold up.

What Is Type D, and Why Does It Matter?

The “D” in Type D stands for “distressed.” Baylor University’s Adam O’Riordan and colleagues (2023), who conducted the review, further define people with Type D as exhibiting the two components of negative affectivity, or sadness and anxiety , and social inhibition, the tendency to push aside the emotions they feel when they’re with other people.

Type D was originally identified in cardiac patients, and in prior reviews of the literature, stood up to scientific scrutiny. Cardiac patients with this personality, in at least a majority of studies conducted, had twice the risk of dying, and as a result, European Guidelines for Cardiovascular Disease Prevention, as cited by O’Riordan et al., include Type D as a “psychosocial risk factor to be assessed in clinical practice.”

People with Type D personalities, prior researchers find, have poorer lifestyle habits such as less frequent physical activity and unhealthy eating. However, this would not be enough to predispose them to higher mortality risk. Because they perceive life events to be more stressful than do the non-Type D people, their bodies release more stress hormones . This “cardiovascular reactivity” hypothesis proposes that this chronic overarousal causes such conditions as hypertension.

Another counter-proposal suggests that it’s the opposite, an under-reactivity, which contributes to Type D’s negative effects on the body. This tendency, a blunted way of dealing with stress, shows up in other research on the ways that Type D people respond to experimentally induced stress. Perhaps it is what the Baylor U. researchers call “homeostatic dysregulation” that accounts for Type D’s harmful health effects.

Testing Type D’s Link to Health

Using standard methods of conducting systematic reviews, O’Riordan et al. began with a set of 401 studies, which, after eliminating those that didn’t make the grade, led to a final collection of 14 studies averaging 99 participants each. The authors rated each study on its methodological quality on a 1 to 9 scale; the final studies included in the review received scores of 5.64 for those reporting significant effects, and 5 for those reporting null effects.

The physiological criteria included measures such as systolic blood pressure (SBP), diastolic blood pressure (DBP), heart rate (HR), cardiac output (CO), or total peripheral resistance reactivity (changes in the arteries).

You can test yourself on your own Type D tendencies with these items (0=False, 4=True).

Negative affectivity:

  • I often make a fuss about unimportant things.
  • I often feel unhappy.
  • I am often irritated.
  • I take a gloomy view of things.
  • I often find myself worrying about something.
  • I am often down in the dumps.

Social inhibition:

  • I make contact easily when I meet people (R).
  • I often talk to strangers (R).
  • I often feel inhibited in social interactions.
  • I find it hard to start a conversation.
  • I am a closed kind of person.
  • I would rather keep other people at a distance.
  • When socializing, I don't find the right things to talk about.

Individuals who obtain a score of 10 or more on both scales are classified as Type D.

The Baylor U. review sought to examine the contributions of sex and the importance of a social stressor to the Type D-cardiac reactivity equation. High social stressors included experimental manipulations requiring direct and reciprocal communication between the participant and another person, such as giving a speech, and/or receiving negative social feedback or evaluation after an experimental task.

which statement is a valid scientific hypothesis

Overall, Type D personality was associated with lower blood pressure and HR reactivity across studies, which would support the “blunted” hypothesis about Type D’s effect on cardiovascular functioning. However, consistent with the prior literature, sex and the nature of the social situation did make a difference in this overall effect.

The authors go on to explain how blunting could be just as bad as hyperarousal, depending on the nature of the stress. As they note, this is a “suboptimal” response in the parts of the brain responsible for “motivational and behavioral regulation.” When engaging in stressful tasks, you want these brain regions to be performing, not dampening. Otherwise, you will experience “withdrawal and disengagement.”

There may be, however, a bright side to this form of withdrawal. Might it not be healthy to settle down if there’s no social pressure on you to perform? Now, though, sex might come into play. Women may be more likely to internalize their feelings of stress than men due to differing socialization in confrontations with stress. However, women are also more likely to express their emotions than men, particularly in social situations.

All in all, the differences across study findings led the authors to conclude that future studies should investigate prospectively, rather than through correlational studies, whether being Type D and having blunted responses to social stressors is healthy or pathological, and whether this varies by sex.

Paying Attention to Your Emotions

The strength of this comprehensive review was its attention to nuanced detail and experimental rigor. Rather than make a blanket statement that it’s always good or always bad to be Type D, the Baylor U. researchers show that the answer is “It depends.” Unlike the Type A researchers, who tended to make broad (and unsubstantiated) claims, O’Riordan et al. showed that Type D’s effects depended on which situations and for whom the cardiovascular responses were observed.

There are practical implications of these findings. By identifying your own Type D tendencies, you can use the “data” you collect across your own daily interactions. If someone is evaluating you, are you able to mobilize and rise to the occasion, or do you retreat and let your emotions eat away at you? On the other hand, when there’s no pressure, do you allow yourself to relax and focus on doing your best?

To sum up, personality can affect your health, but not in a direct and necessarily obvious way. Learning to tune into your own reactivity can help you find ways to keep stress at bay and focus instead on your ability to thrive.

Denollet, J. (2005). DS14: Standard Assessment of Negative Affectivity, Social Inhibition, and Type D Personality. Psychosomatic Medicine , 67 (1), 89–97. doi:10.1097/01.psy.0000149256.81953.49

O'Riordan, A., Gallagher, S., & Howard, S. (2023). Type D personality and cardiovascular reactivity to acute psychological stress: A systematic review and meta-analysis. Health Psychology, 42 (9), 628–641. https://doi.org/10.1037/hea0001328

Susan Krauss Whitbourne Ph.D.

Susan Krauss Whitbourne, Ph.D. , is a Professor Emerita of Psychological and Brain Sciences at the University of Massachusetts Amherst. Her latest book is The Search for Fulfillment.

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At any moment, someone’s aggravating behavior or our own bad luck can set us off on an emotional spiral that threatens to derail our entire day. Here’s how we can face our triggers with less reactivity so that we can get on with our lives.

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