hypothesis and functions

Scientific Methods

What is Hypothesis?

We have heard of many hypotheses which have led to great inventions in science. Assumptions that are made on the basis of some evidence are known as hypotheses. In this article, let us learn in detail about the hypothesis and the type of hypothesis with examples.

A hypothesis is an assumption that is made based on some evidence. This is the initial point of any investigation that translates the research questions into predictions. It includes components like variables, population and the relation between the variables. A research hypothesis is a hypothesis that is used to test the relationship between two or more variables.

Characteristics of Hypothesis

Following are the characteristics of the hypothesis:

The hypothesis should be clear and precise to consider it to be reliable.
If the hypothesis is a relational hypothesis, then it should be stating the relationship between variables.
The hypothesis must be specific and should have scope for conducting more tests.
The way of explanation of the hypothesis must be very simple and it should also be understood that the simplicity of the hypothesis is not related to its significance.

Sources of Hypothesis

Following are the sources of hypothesis:

The resemblance between the phenomenon.
Observations from past studies, present-day experiences and from the competitors.
Scientific theories.
General patterns that influence the thinking process of people.

Types of Hypothesis

There are six forms of hypothesis and they are:

Simple hypothesis
Complex hypothesis
Directional hypothesis
Non-directional hypothesis
Null hypothesis
Associative and casual hypothesis

Simple Hypothesis

It shows a relationship between one dependent variable and a single independent variable. For example – If you eat more vegetables, you will lose weight faster. Here, eating more vegetables is an independent variable, while losing weight is the dependent variable.

Complex Hypothesis

It shows the relationship between two or more dependent variables and two or more independent variables. Eating more vegetables and fruits leads to weight loss, glowing skin, and reduces the risk of many diseases such as heart disease.

Directional Hypothesis

It shows how a researcher is intellectual and committed to a particular outcome. The relationship between the variables can also predict its nature. For example- children aged four years eating proper food over a five-year period are having higher IQ levels than children not having a proper meal. This shows the effect and direction of the effect.

Non-directional Hypothesis

It is used when there is no theory involved. It is a statement that a relationship exists between two variables, without predicting the exact nature (direction) of the relationship.

Null Hypothesis

It provides a statement which is contrary to the hypothesis. It’s a negative statement, and there is no relationship between independent and dependent variables. The symbol is denoted by “H O ”.

Associative and Causal Hypothesis

Associative hypothesis occurs when there is a change in one variable resulting in a change in the other variable. Whereas, the causal hypothesis proposes a cause and effect interaction between two or more variables.

Examples of Hypothesis

Following are the examples of hypotheses based on their types:

Consumption of sugary drinks every day leads to obesity is an example of a simple hypothesis.
All lilies have the same number of petals is an example of a null hypothesis.
If a person gets 7 hours of sleep, then he will feel less fatigue than if he sleeps less. It is an example of a directional hypothesis.

Functions of Hypothesis

Following are the functions performed by the hypothesis:

Hypothesis helps in making an observation and experiments possible.
It becomes the start point for the investigation.
Hypothesis helps in verifying the observations.
It helps in directing the inquiries in the right direction.

How will Hypothesis help in the Scientific Method?

Researchers use hypotheses to put down their thoughts directing how the experiment would take place. Following are the steps that are involved in the scientific method:

Formation of question
Doing background research
Creation of hypothesis
Designing an experiment
Collection of data
Result analysis
Summarizing the experiment
Communicating the results

Frequently Asked Questions – FAQs

What is hypothesis.

A hypothesis is an assumption made based on some evidence.

Give an example of simple hypothesis?

What are the types of hypothesis.

Types of hypothesis are:

Associative and Casual hypothesis

State true or false: Hypothesis is the initial point of any investigation that translates the research questions into a prediction.

Define complex hypothesis..

A complex hypothesis shows the relationship between two or more dependent variables and two or more independent variables.

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Home » What is a Hypothesis – Types, Examples and Writing Guide

What is a Hypothesis – Types, Examples and Writing Guide

Table of Contents

Definition:

Hypothesis is an educated guess or proposed explanation for a phenomenon, based on some initial observations or data. It is a tentative statement that can be tested and potentially proven or disproven through further investigation and experimentation.

Hypothesis is often used in scientific research to guide the design of experiments and the collection and analysis of data. It is an essential element of the scientific method, as it allows researchers to make predictions about the outcome of their experiments and to test those predictions to determine their accuracy.

Types of Hypothesis

Types of Hypothesis are as follows:

Research Hypothesis

A research hypothesis is a statement that predicts a relationship between variables. It is usually formulated as a specific statement that can be tested through research, and it is often used in scientific research to guide the design of experiments.

Null Hypothesis

The null hypothesis is a statement that assumes there is no significant difference or relationship between variables. It is often used as a starting point for testing the research hypothesis, and if the results of the study reject the null hypothesis, it suggests that there is a significant difference or relationship between variables.

Alternative Hypothesis

An alternative hypothesis is a statement that assumes there is a significant difference or relationship between variables. It is often used as an alternative to the null hypothesis and is tested against the null hypothesis to determine which statement is more accurate.

Directional Hypothesis

A directional hypothesis is a statement that predicts the direction of the relationship between variables. For example, a researcher might predict that increasing the amount of exercise will result in a decrease in body weight.

Non-directional Hypothesis

A non-directional hypothesis is a statement that predicts the relationship between variables but does not specify the direction. For example, a researcher might predict that there is a relationship between the amount of exercise and body weight, but they do not specify whether increasing or decreasing exercise will affect body weight.

Statistical Hypothesis

A statistical hypothesis is a statement that assumes a particular statistical model or distribution for the data. It is often used in statistical analysis to test the significance of a particular result.

Composite Hypothesis

A composite hypothesis is a statement that assumes more than one condition or outcome. It can be divided into several sub-hypotheses, each of which represents a different possible outcome.

Empirical Hypothesis

An empirical hypothesis is a statement that is based on observed phenomena or data. It is often used in scientific research to develop theories or models that explain the observed phenomena.

Simple Hypothesis

A simple hypothesis is a statement that assumes only one outcome or condition. It is often used in scientific research to test a single variable or factor.

Complex Hypothesis

A complex hypothesis is a statement that assumes multiple outcomes or conditions. It is often used in scientific research to test the effects of multiple variables or factors on a particular outcome.

Applications of Hypothesis

Hypotheses are used in various fields to guide research and make predictions about the outcomes of experiments or observations. Here are some examples of how hypotheses are applied in different fields:

Science : In scientific research, hypotheses are used to test the validity of theories and models that explain natural phenomena. For example, a hypothesis might be formulated to test the effects of a particular variable on a natural system, such as the effects of climate change on an ecosystem.
Medicine : In medical research, hypotheses are used to test the effectiveness of treatments and therapies for specific conditions. For example, a hypothesis might be formulated to test the effects of a new drug on a particular disease.
Psychology : In psychology, hypotheses are used to test theories and models of human behavior and cognition. For example, a hypothesis might be formulated to test the effects of a particular stimulus on the brain or behavior.
Sociology : In sociology, hypotheses are used to test theories and models of social phenomena, such as the effects of social structures or institutions on human behavior. For example, a hypothesis might be formulated to test the effects of income inequality on crime rates.
Business : In business research, hypotheses are used to test the validity of theories and models that explain business phenomena, such as consumer behavior or market trends. For example, a hypothesis might be formulated to test the effects of a new marketing campaign on consumer buying behavior.
Engineering : In engineering, hypotheses are used to test the effectiveness of new technologies or designs. For example, a hypothesis might be formulated to test the efficiency of a new solar panel design.

How to write a Hypothesis

Here are the steps to follow when writing a hypothesis:

Identify the Research Question

The first step is to identify the research question that you want to answer through your study. This question should be clear, specific, and focused. It should be something that can be investigated empirically and that has some relevance or significance in the field.

Conduct a Literature Review

Before writing your hypothesis, it’s essential to conduct a thorough literature review to understand what is already known about the topic. This will help you to identify the research gap and formulate a hypothesis that builds on existing knowledge.

Determine the Variables

The next step is to identify the variables involved in the research question. A variable is any characteristic or factor that can vary or change. There are two types of variables: independent and dependent. The independent variable is the one that is manipulated or changed by the researcher, while the dependent variable is the one that is measured or observed as a result of the independent variable.

Formulate the Hypothesis

Based on the research question and the variables involved, you can now formulate your hypothesis. A hypothesis should be a clear and concise statement that predicts the relationship between the variables. It should be testable through empirical research and based on existing theory or evidence.

Write the Null Hypothesis

The null hypothesis is the opposite of the alternative hypothesis, which is the hypothesis that you are testing. The null hypothesis states that there is no significant difference or relationship between the variables. It is important to write the null hypothesis because it allows you to compare your results with what would be expected by chance.

Refine the Hypothesis

After formulating the hypothesis, it’s important to refine it and make it more precise. This may involve clarifying the variables, specifying the direction of the relationship, or making the hypothesis more testable.

Examples of Hypothesis

Here are a few examples of hypotheses in different fields:

Psychology : “Increased exposure to violent video games leads to increased aggressive behavior in adolescents.”
Biology : “Higher levels of carbon dioxide in the atmosphere will lead to increased plant growth.”
Sociology : “Individuals who grow up in households with higher socioeconomic status will have higher levels of education and income as adults.”
Education : “Implementing a new teaching method will result in higher student achievement scores.”
Marketing : “Customers who receive a personalized email will be more likely to make a purchase than those who receive a generic email.”
Physics : “An increase in temperature will cause an increase in the volume of a gas, assuming all other variables remain constant.”
Medicine : “Consuming a diet high in saturated fats will increase the risk of developing heart disease.”

Purpose of Hypothesis

The purpose of a hypothesis is to provide a testable explanation for an observed phenomenon or a prediction of a future outcome based on existing knowledge or theories. A hypothesis is an essential part of the scientific method and helps to guide the research process by providing a clear focus for investigation. It enables scientists to design experiments or studies to gather evidence and data that can support or refute the proposed explanation or prediction.

The formulation of a hypothesis is based on existing knowledge, observations, and theories, and it should be specific, testable, and falsifiable. A specific hypothesis helps to define the research question, which is important in the research process as it guides the selection of an appropriate research design and methodology. Testability of the hypothesis means that it can be proven or disproven through empirical data collection and analysis. Falsifiability means that the hypothesis should be formulated in such a way that it can be proven wrong if it is incorrect.

In addition to guiding the research process, the testing of hypotheses can lead to new discoveries and advancements in scientific knowledge. When a hypothesis is supported by the data, it can be used to develop new theories or models to explain the observed phenomenon. When a hypothesis is not supported by the data, it can help to refine existing theories or prompt the development of new hypotheses to explain the phenomenon.

When to use Hypothesis

Here are some common situations in which hypotheses are used:

In scientific research , hypotheses are used to guide the design of experiments and to help researchers make predictions about the outcomes of those experiments.
In social science research , hypotheses are used to test theories about human behavior, social relationships, and other phenomena.
I n business , hypotheses can be used to guide decisions about marketing, product development, and other areas. For example, a hypothesis might be that a new product will sell well in a particular market, and this hypothesis can be tested through market research.

Characteristics of Hypothesis

Here are some common characteristics of a hypothesis:

Testable : A hypothesis must be able to be tested through observation or experimentation. This means that it must be possible to collect data that will either support or refute the hypothesis.
Falsifiable : A hypothesis must be able to be proven false if it is not supported by the data. If a hypothesis cannot be falsified, then it is not a scientific hypothesis.
Clear and concise : A hypothesis should be stated in a clear and concise manner so that it can be easily understood and tested.
Based on existing knowledge : A hypothesis should be based on existing knowledge and research in the field. It should not be based on personal beliefs or opinions.
Specific : A hypothesis should be specific in terms of the variables being tested and the predicted outcome. This will help to ensure that the research is focused and well-designed.
Tentative: A hypothesis is a tentative statement or assumption that requires further testing and evidence to be confirmed or refuted. It is not a final conclusion or assertion.
Relevant : A hypothesis should be relevant to the research question or problem being studied. It should address a gap in knowledge or provide a new perspective on the issue.

Advantages of Hypothesis

Hypotheses have several advantages in scientific research and experimentation:

Guides research: A hypothesis provides a clear and specific direction for research. It helps to focus the research question, select appropriate methods and variables, and interpret the results.
Predictive powe r: A hypothesis makes predictions about the outcome of research, which can be tested through experimentation. This allows researchers to evaluate the validity of the hypothesis and make new discoveries.
Facilitates communication: A hypothesis provides a common language and framework for scientists to communicate with one another about their research. This helps to facilitate the exchange of ideas and promotes collaboration.
Efficient use of resources: A hypothesis helps researchers to use their time, resources, and funding efficiently by directing them towards specific research questions and methods that are most likely to yield results.
Provides a basis for further research: A hypothesis that is supported by data provides a basis for further research and exploration. It can lead to new hypotheses, theories, and discoveries.
Increases objectivity: A hypothesis can help to increase objectivity in research by providing a clear and specific framework for testing and interpreting results. This can reduce bias and increase the reliability of research findings.

Limitations of Hypothesis

Some Limitations of the Hypothesis are as follows:

Limited to observable phenomena: Hypotheses are limited to observable phenomena and cannot account for unobservable or intangible factors. This means that some research questions may not be amenable to hypothesis testing.
May be inaccurate or incomplete: Hypotheses are based on existing knowledge and research, which may be incomplete or inaccurate. This can lead to flawed hypotheses and erroneous conclusions.
May be biased: Hypotheses may be biased by the researcher’s own beliefs, values, or assumptions. This can lead to selective interpretation of data and a lack of objectivity in research.
Cannot prove causation: A hypothesis can only show a correlation between variables, but it cannot prove causation. This requires further experimentation and analysis.
Limited to specific contexts: Hypotheses are limited to specific contexts and may not be generalizable to other situations or populations. This means that results may not be applicable in other contexts or may require further testing.
May be affected by chance : Hypotheses may be affected by chance or random variation, which can obscure or distort the true relationship between variables.

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On This Page:

A research hypothesis, in its plural form “hypotheses,” is a specific, testable prediction about the anticipated results of a study, established at its outset. It is a key component of the scientific method .

Hypotheses connect theory to data and guide the research process towards expanding scientific understanding

Some key points about hypotheses:

A hypothesis expresses an expected pattern or relationship. It connects the variables under investigation.
It is stated in clear, precise terms before any data collection or analysis occurs. This makes the hypothesis testable.
A hypothesis must be falsifiable. It should be possible, even if unlikely in practice, to collect data that disconfirms rather than supports the hypothesis.
Hypotheses guide research. Scientists design studies to explicitly evaluate hypotheses about how nature works.
For a hypothesis to be valid, it must be testable against empirical evidence. The evidence can then confirm or disprove the testable predictions.
Hypotheses are informed by background knowledge and observation, but go beyond what is already known to propose an explanation of how or why something occurs.

Predictions typically arise from a thorough knowledge of the research literature, curiosity about real-world problems or implications, and integrating this to advance theory. They build on existing literature while providing new insight.

Types of Research Hypotheses

Alternative hypothesis.

The research hypothesis is often called the alternative or experimental hypothesis in experimental research.

It typically suggests a potential relationship between two key variables: the independent variable, which the researcher manipulates, and the dependent variable, which is measured based on those changes.

The alternative hypothesis states a relationship exists between the two variables being studied (one variable affects the other).

A hypothesis is a testable statement or prediction about the relationship between two or more variables. It is a key component of the scientific method. Some key points about hypotheses:

Important hypotheses lead to predictions that can be tested empirically. The evidence can then confirm or disprove the testable predictions.

In summary, a hypothesis is a precise, testable statement of what researchers expect to happen in a study and why. Hypotheses connect theory to data and guide the research process towards expanding scientific understanding.

An experimental hypothesis predicts what change(s) will occur in the dependent variable when the independent variable is manipulated.

It states that the results are not due to chance and are significant in supporting the theory being investigated.

The alternative hypothesis can be directional, indicating a specific direction of the effect, or non-directional, suggesting a difference without specifying its nature. It’s what researchers aim to support or demonstrate through their study.

Null Hypothesis

The null hypothesis states no relationship exists between the two variables being studied (one variable does not affect the other). There will be no changes in the dependent variable due to manipulating the independent variable.

It states results are due to chance and are not significant in supporting the idea being investigated.

The null hypothesis, positing no effect or relationship, is a foundational contrast to the research hypothesis in scientific inquiry. It establishes a baseline for statistical testing, promoting objectivity by initiating research from a neutral stance.

Many statistical methods are tailored to test the null hypothesis, determining the likelihood of observed results if no true effect exists.

This dual-hypothesis approach provides clarity, ensuring that research intentions are explicit, and fosters consistency across scientific studies, enhancing the standardization and interpretability of research outcomes.

Nondirectional Hypothesis

A non-directional hypothesis, also known as a two-tailed hypothesis, predicts that there is a difference or relationship between two variables but does not specify the direction of this relationship.

It merely indicates that a change or effect will occur without predicting which group will have higher or lower values.

For example, “There is a difference in performance between Group A and Group B” is a non-directional hypothesis.

Directional Hypothesis

A directional (one-tailed) hypothesis predicts the nature of the effect of the independent variable on the dependent variable. It predicts in which direction the change will take place. (i.e., greater, smaller, less, more)

It specifies whether one variable is greater, lesser, or different from another, rather than just indicating that there’s a difference without specifying its nature.

For example, “Exercise increases weight loss” is a directional hypothesis.

Falsifiability

The Falsification Principle, proposed by Karl Popper , is a way of demarcating science from non-science. It suggests that for a theory or hypothesis to be considered scientific, it must be testable and irrefutable.

Falsifiability emphasizes that scientific claims shouldn’t just be confirmable but should also have the potential to be proven wrong.

It means that there should exist some potential evidence or experiment that could prove the proposition false.

However many confirming instances exist for a theory, it only takes one counter observation to falsify it. For example, the hypothesis that “all swans are white,” can be falsified by observing a black swan.

For Popper, science should attempt to disprove a theory rather than attempt to continually provide evidence to support a research hypothesis.

Can a Hypothesis be Proven?

Hypotheses make probabilistic predictions. They state the expected outcome if a particular relationship exists. However, a study result supporting a hypothesis does not definitively prove it is true.

All studies have limitations. There may be unknown confounding factors or issues that limit the certainty of conclusions. Additional studies may yield different results.

In science, hypotheses can realistically only be supported with some degree of confidence, not proven. The process of science is to incrementally accumulate evidence for and against hypothesized relationships in an ongoing pursuit of better models and explanations that best fit the empirical data. But hypotheses remain open to revision and rejection if that is where the evidence leads.

Disproving a hypothesis is definitive. Solid disconfirmatory evidence will falsify a hypothesis and require altering or discarding it based on the evidence.
However, confirming evidence is always open to revision. Other explanations may account for the same results, and additional or contradictory evidence may emerge over time.

We can never 100% prove the alternative hypothesis. Instead, we see if we can disprove, or reject the null hypothesis.

If we reject the null hypothesis, this doesn’t mean that our alternative hypothesis is correct but does support the alternative/experimental hypothesis.

Upon analysis of the results, an alternative hypothesis can be rejected or supported, but it can never be proven to be correct. We must avoid any reference to results proving a theory as this implies 100% certainty, and there is always a chance that evidence may exist which could refute a theory.

How to Write a Hypothesis

Identify variables . The researcher manipulates the independent variable and the dependent variable is the measured outcome.
Operationalized the variables being investigated . Operationalization of a hypothesis refers to the process of making the variables physically measurable or testable, e.g. if you are about to study aggression, you might count the number of punches given by participants.
Decide on a direction for your prediction . If there is evidence in the literature to support a specific effect of the independent variable on the dependent variable, write a directional (one-tailed) hypothesis. If there are limited or ambiguous findings in the literature regarding the effect of the independent variable on the dependent variable, write a non-directional (two-tailed) hypothesis.
Make it Testable : Ensure your hypothesis can be tested through experimentation or observation. It should be possible to prove it false (principle of falsifiability).
Clear & concise language . A strong hypothesis is concise (typically one to two sentences long), and formulated using clear and straightforward language, ensuring it’s easily understood and testable.

Consider a hypothesis many teachers might subscribe to: students work better on Monday morning than on Friday afternoon (IV=Day, DV= Standard of work).

Now, if we decide to study this by giving the same group of students a lesson on a Monday morning and a Friday afternoon and then measuring their immediate recall of the material covered in each session, we would end up with the following:

The alternative hypothesis states that students will recall significantly more information on a Monday morning than on a Friday afternoon.
The null hypothesis states that there will be no significant difference in the amount recalled on a Monday morning compared to a Friday afternoon. Any difference will be due to chance or confounding factors.

More Examples

Memory : Participants exposed to classical music during study sessions will recall more items from a list than those who studied in silence.
Social Psychology : Individuals who frequently engage in social media use will report higher levels of perceived social isolation compared to those who use it infrequently.
Developmental Psychology : Children who engage in regular imaginative play have better problem-solving skills than those who don’t.
Clinical Psychology : Cognitive-behavioral therapy will be more effective in reducing symptoms of anxiety over a 6-month period compared to traditional talk therapy.
Cognitive Psychology : Individuals who multitask between various electronic devices will have shorter attention spans on focused tasks than those who single-task.
Health Psychology : Patients who practice mindfulness meditation will experience lower levels of chronic pain compared to those who don’t meditate.
Organizational Psychology : Employees in open-plan offices will report higher levels of stress than those in private offices.
Behavioral Psychology : Rats rewarded with food after pressing a lever will press it more frequently than rats who receive no reward.

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What is a scientific hypothesis?

It's the initial building block in the scientific method.

A girl looks at plants in a test tube for a science experiment. What's her scientific hypothesis?

Hypothesis basics

What makes a hypothesis testable.

Types of hypotheses
Hypothesis versus theory

Additional resources

Bibliography.

A scientific hypothesis is a tentative, testable explanation for a phenomenon in the natural world. It's the initial building block in the scientific method . Many describe it as an "educated guess" based on prior knowledge and observation. While this is true, a hypothesis is more informed than a guess. While an "educated guess" suggests a random prediction based on a person's expertise, developing a hypothesis requires active observation and background research.

The basic idea of a hypothesis is that there is no predetermined outcome. For a solution to be termed a scientific hypothesis, it has to be an idea that can be supported or refuted through carefully crafted experimentation or observation. This concept, called falsifiability and testability, was advanced in the mid-20th century by Austrian-British philosopher Karl Popper in his famous book "The Logic of Scientific Discovery" (Routledge, 1959).

A key function of a hypothesis is to derive predictions about the results of future experiments and then perform those experiments to see whether they support the predictions.

A hypothesis is usually written in the form of an if-then statement, which gives a possibility (if) and explains what may happen because of the possibility (then). The statement could also include "may," according to California State University, Bakersfield .

Here are some examples of hypothesis statements:

If garlic repels fleas, then a dog that is given garlic every day will not get fleas.
If sugar causes cavities, then people who eat a lot of candy may be more prone to cavities.
If ultraviolet light can damage the eyes, then maybe this light can cause blindness.

A useful hypothesis should be testable and falsifiable. That means that it should be possible to prove it wrong. A theory that can't be proved wrong is nonscientific, according to Karl Popper's 1963 book " Conjectures and Refutations ."

An example of an untestable statement is, "Dogs are better than cats." That's because the definition of "better" is vague and subjective. However, an untestable statement can be reworded to make it testable. For example, the previous statement could be changed to this: "Owning a dog is associated with higher levels of physical fitness than owning a cat." With this statement, the researcher can take measures of physical fitness from dog and cat owners and compare the two.

Types of scientific hypotheses

Elementary-age students study alternative energy using homemade windmills during public school science class.

In an experiment, researchers generally state their hypotheses in two ways. The null hypothesis predicts that there will be no relationship between the variables tested, or no difference between the experimental groups. The alternative hypothesis predicts the opposite: that there will be a difference between the experimental groups. This is usually the hypothesis scientists are most interested in, according to the University of Miami .

For example, a null hypothesis might state, "There will be no difference in the rate of muscle growth between people who take a protein supplement and people who don't." The alternative hypothesis would state, "There will be a difference in the rate of muscle growth between people who take a protein supplement and people who don't."

If the results of the experiment show a relationship between the variables, then the null hypothesis has been rejected in favor of the alternative hypothesis, according to the book " Research Methods in Psychology " (BCcampus, 2015).

There are other ways to describe an alternative hypothesis. The alternative hypothesis above does not specify a direction of the effect, only that there will be a difference between the two groups. That type of prediction is called a two-tailed hypothesis. If a hypothesis specifies a certain direction — for example, that people who take a protein supplement will gain more muscle than people who don't — it is called a one-tailed hypothesis, according to William M. K. Trochim , a professor of Policy Analysis and Management at Cornell University.

Sometimes, errors take place during an experiment. These errors can happen in one of two ways. A type I error is when the null hypothesis is rejected when it is true. This is also known as a false positive. A type II error occurs when the null hypothesis is not rejected when it is false. This is also known as a false negative, according to the University of California, Berkeley .

A hypothesis can be rejected or modified, but it can never be proved correct 100% of the time. For example, a scientist can form a hypothesis stating that if a certain type of tomato has a gene for red pigment, that type of tomato will be red. During research, the scientist then finds that each tomato of this type is red. Though the findings confirm the hypothesis, there may be a tomato of that type somewhere in the world that isn't red. Thus, the hypothesis is true, but it may not be true 100% of the time.

Scientific theory vs. scientific hypothesis

The best hypotheses are simple. They deal with a relatively narrow set of phenomena. But theories are broader; they generally combine multiple hypotheses into a general explanation for a wide range of phenomena, according to the University of California, Berkeley . For example, a hypothesis might state, "If animals adapt to suit their environments, then birds that live on islands with lots of seeds to eat will have differently shaped beaks than birds that live on islands with lots of insects to eat." After testing many hypotheses like these, Charles Darwin formulated an overarching theory: the theory of evolution by natural selection.

"Theories are the ways that we make sense of what we observe in the natural world," Tanner said. "Theories are structures of ideas that explain and interpret facts."

Read more about writing a hypothesis, from the American Medical Writers Association.
Find out why a hypothesis isn't always necessary in science, from The American Biology Teacher.
Learn about null and alternative hypotheses, from Prof. Essa on YouTube .

Encyclopedia Britannica. Scientific Hypothesis. Jan. 13, 2022. https://www.britannica.com/science/scientific-hypothesis

Karl Popper, "The Logic of Scientific Discovery," Routledge, 1959.

California State University, Bakersfield, "Formatting a testable hypothesis." https://www.csub.edu/~ddodenhoff/Bio100/Bio100sp04/formattingahypothesis.htm

Karl Popper, "Conjectures and Refutations," Routledge, 1963.

Price, P., Jhangiani, R., & Chiang, I., "Research Methods of Psychology — 2nd Canadian Edition," BCcampus, 2015.‌

University of Miami, "The Scientific Method" http://www.bio.miami.edu/dana/161/evolution/161app1_scimethod.pdf

William M.K. Trochim, "Research Methods Knowledge Base," https://conjointly.com/kb/hypotheses-explained/

University of California, Berkeley, "Multiple Hypothesis Testing and False Discovery Rate" https://www.stat.berkeley.edu/~hhuang/STAT141/Lecture-FDR.pdf

University of California, Berkeley, "Science at multiple levels" https://undsci.berkeley.edu/article/0_0_0/howscienceworks_19

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What Is Hypothesis?

A scientific hypothesis is a foundational element of the scientific method . It’s a testable statement proposing a potential explanation for natural phenomena. The term hypothesis means “little theory” . A hypothesis is a short statement that can be tested and gives a possible reason for a phenomenon or a possible link between two variables . In the setting of scientific research, a hypothesis is a tentative explanation or statement that can be proven wrong and is used to guide experiments and empirical research.

It is an important part of the scientific method because it gives a basis for planning tests, gathering data, and judging evidence to see if it is true and could help us understand how natural things work. Several hypotheses can be tested in the real world, and the results of careful and systematic observation and analysis can be used to support, reject, or improve them.

Researchers and scientists often use the word hypothesis to refer to this educated guess . These hypotheses are firmly established based on scientific principles and the rigorous testing of new technology and experiments .

For example, in astrophysics, the Big Bang Theory is a working hypothesis that explains the origins of the universe and considers it as a natural phenomenon. It is among the most prominent scientific hypotheses in the field.

“The scientific method: steps, terms, and examples” by Scishow:

Biology definition: A hypothesis  is a supposition or tentative explanation for (a group of) phenomena, (a set of) facts, or a scientific inquiry that may be tested, verified or answered by further investigation or methodological experiment. It is like a scientific guess . It’s an idea or prediction that scientists make before they do experiments. They use it to guess what might happen and then test it to see if they were right. It’s like a smart guess that helps them learn new things. A scientific hypothesis that has been verified through scientific experiment and research may well be considered a scientific theory .

Etymology: The word “hypothesis” comes from the Greek word “hupothesis,” which means “a basis” or “a supposition.” It combines “hupo” (under) and “thesis” (placing). Synonym: proposition; assumption; conjecture; postulate Compare:   theory See also: null hypothesis

Characteristics Of Hypothesis

A useful hypothesis must have the following qualities:

It should never be written as a question.
You should be able to test it in the real world to see if it’s right or wrong.
It needs to be clear and exact.
It should list the factors that will be used to figure out the relationship.
It should only talk about one thing. You can make a theory in either a descriptive or form of relationship.
It shouldn’t go against any natural rule that everyone knows is true. Verification will be done well with the tools and methods that are available.
It should be written in as simple a way as possible so that everyone can understand it.
It must explain what happened to make an answer necessary.
It should be testable in a fair amount of time.
It shouldn’t say different things.

Sources Of Hypothesis

Sources of hypothesis are:

Patterns of similarity between the phenomenon under investigation and existing hypotheses.
Insights derived from prior research, concurrent observations, and insights from opposing perspectives.
The formulations are derived from accepted scientific theories and proposed by researchers.
In research, it’s essential to consider hypothesis as different subject areas may require various hypotheses (plural form of hypothesis). Researchers also establish a significance level to determine the strength of evidence supporting a hypothesis.
Individual cognitive processes also contribute to the formation of hypotheses.

One hypothesis is a tentative explanation for an observation or phenomenon. It is based on prior knowledge and understanding of the world, and it can be tested by gathering and analyzing data. Observed facts are the data that are collected to test a hypothesis. They can support or refute the hypothesis.

For example, the hypothesis that “eating more fruits and vegetables will improve your health” can be tested by gathering data on the health of people who eat different amounts of fruits and vegetables. If the people who eat more fruits and vegetables are healthier than those who eat less fruits and vegetables, then the hypothesis is supported.

Hypotheses are essential for scientific inquiry. They help scientists to focus their research, to design experiments, and to interpret their results. They are also essential for the development of scientific theories.

Types Of Hypothesis

In research, you typically encounter two types of hypothesis: the alternative hypothesis (which proposes a relationship between variables) and the null hypothesis (which suggests no relationship).

Simple Hypothesis

It illustrates the association between one dependent variable and one independent variable. For instance, if you consume more vegetables, you will lose weight more quickly. Here, increasing vegetable consumption is the independent variable, while weight loss is the dependent variable.

Complex Hypothesis

It exhibits the relationship between at least two dependent variables and at least two independent variables. Eating more vegetables and fruits results in weight loss, radiant skin, and a decreased risk of numerous diseases, including heart disease.

Directional Hypothesis

It shows that a researcher wants to reach a certain goal. The way the factors are related can also tell us about their nature. For example, four-year-old children who eat well over a time of five years have a higher IQ than children who don’t eat well. This shows what happened and how it happened.

Non-directional Hypothesis

When there is no theory involved, it is used. It is a statement that there is a connection between two variables, but it doesn’t say what that relationship is or which way it goes.

Null Hypothesis

It says something that goes against the theory. It’s a statement that says something is not true, and there is no link between the independent and dependent factors. “H 0 ” represents the null hypothesis.

Associative and Causal Hypothesis

When a change in one variable causes a change in the other variable, this is called the associative hypothesis . The causal hypothesis, on the other hand, says that there is a cause-and-effect relationship between two or more factors.

Examples Of Hypothesis

Examples of simple hypotheses:

Students who consume breakfast before taking a math test will have a better overall performance than students who do not consume breakfast.
Students who experience test anxiety before an English examination will get lower scores than students who do not experience test anxiety.
Motorists who talk on the phone while driving will be more likely to make errors on a driving course than those who do not talk on the phone, is a statement that suggests that drivers who talk on the phone while driving are more likely to make mistakes.

Examples of a complex hypothesis:

Individuals who consume a lot of sugar and don’t get much exercise are at an increased risk of developing depression.
Younger people who are routinely exposed to green, outdoor areas have better subjective well-being than older adults who have limited exposure to green spaces, according to a new study.
Increased levels of air pollution led to higher rates of respiratory illnesses, which in turn resulted in increased costs for healthcare for the affected communities.

Examples of Directional Hypothesis:

The crop yield will go up a lot if the amount of fertilizer is increased.
Patients who have surgery and are exposed to more stress will need more time to get better.
Increasing the frequency of brand advertising on social media will lead to a significant increase in brand awareness among the target audience.

Examples of Non-Directional Hypothesis (or Two-Tailed Hypothesis):

The test scores of two groups of students are very different from each other.
There is a link between gender and being happy at work.
There is a correlation between the amount of caffeine an individual consumes and the speed with which they react.

Examples of a null hypothesis:

Children who receive a new reading intervention will have scores that are different than students who do not receive the intervention.
The results of a memory recall test will not reveal any significant gap in performance between children and adults.
There is not a significant relationship between the number of hours spent playing video games and academic performance.

Examples of Associative Hypothesis:

There is a link between how many hours you spend studying and how well you do in school.
Drinking sugary drinks is bad for your health as a whole.
There is an association between socioeconomic status and access to quality healthcare services in urban neighborhoods.

Functions Of Hypothesis

The research issue can be understood better with the help of a hypothesis, which is why developing one is crucial. The following are some of the specific roles that a hypothesis plays: (Rashid, Apr 20, 2022)

A hypothesis gives a study a point of concentration. It enlightens us as to the specific characteristics of a study subject we need to look into.
It instructs us on what data to acquire as well as what data we should not collect, giving the study a focal point .
The development of a hypothesis improves objectivity since it enables the establishment of a focal point.
A hypothesis makes it possible for us to contribute to the development of the theory. Because of this, we are in a position to definitively determine what is true and what is untrue .

How will Hypothesis help in the Scientific Method?

The scientific method begins with observation and inquiry about the natural world when formulating research questions. Researchers can refine their observations and queries into specific, testable research questions with the aid of hypothesis. They provide an investigation with a focused starting point.
Hypothesis generate specific predictions regarding the expected outcomes of experiments or observations. These forecasts are founded on the researcher’s current knowledge of the subject. They elucidate what researchers anticipate observing if the hypothesis is true.
Hypothesis direct the design of experiments and data collection techniques. Researchers can use them to determine which variables to measure or manipulate, which data to obtain, and how to conduct systematic and controlled research.
Following the formulation of a hypothesis and the design of an experiment, researchers collect data through observation, measurement, or experimentation. The collected data is used to verify the hypothesis’s predictions.
Hypothesis establish the criteria for evaluating experiment results. The observed data are compared to the predictions generated by the hypothesis. This analysis helps determine whether empirical evidence supports or refutes the hypothesis.
The results of experiments or observations are used to derive conclusions regarding the hypothesis. If the data support the predictions, then the hypothesis is supported. If this is not the case, the hypothesis may be revised or rejected, leading to the formulation of new queries and hypothesis.
The scientific approach is iterative, resulting in new hypothesis and research issues from previous trials. This cycle of hypothesis generation, testing, and refining drives scientific progress.

Importance Of Hypothesis

Hypothesis are testable statements that enable scientists to determine if their predictions are accurate. This assessment is essential to the scientific method, which is based on empirical evidence.
Hypothesis serve as the foundation for designing experiments or data collection techniques. They can be used by researchers to develop protocols and procedures that will produce meaningful results.
Hypothesis hold scientists accountable for their assertions. They establish expectations for what the research should reveal and enable others to assess the validity of the findings.
Hypothesis aid in identifying the most important variables of a study. The variables can then be measured, manipulated, or analyzed to determine their relationships.
Hypothesis assist researchers in allocating their resources efficiently. They ensure that time, money, and effort are spent investigating specific concerns, as opposed to exploring random concepts.
Testing hypothesis contribute to the scientific body of knowledge. Whether or not a hypothesis is supported, the results contribute to our understanding of a phenomenon.
Hypothesis can result in the creation of theories. When supported by substantive evidence, hypothesis can serve as the foundation for larger theoretical frameworks that explain complex phenomena.
Beyond scientific research, hypothesis play a role in the solution of problems in a variety of domains. They enable professionals to make educated assumptions about the causes of problems and to devise solutions.

Research Hypotheses: Did you know that a hypothesis refers to an educated guess or prediction about the outcome of a research study?

It’s like a roadmap guiding researchers towards their destination of knowledge. Just like a compass points north, a well-crafted hypothesis points the way to valuable discoveries in the world of science and inquiry.

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9.1: Introduction to Hypothesis Testing

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Kyle Siegrist
University of Alabama in Huntsville via Random Services

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Basic Theory

Preliminaries.

As usual, our starting point is a random experiment with an underlying sample space and a probability measure $\P$. In the basic statistical model, we have an observable random variable $\bs{X}$ taking values in a set $S$. In general, $\bs{X}$ can have quite a complicated structure. For example, if the experiment is to sample $n$ objects from a population and record various measurements of interest, then \[ \bs{X} = (X_1, X_2, \ldots, X_n) \] where $X_i$ is the vector of measurements for the $i$th object. The most important special case occurs when $(X_1, X_2, \ldots, X_n)$ are independent and identically distributed. In this case, we have a random sample of size $n$ from the common distribution.

The purpose of this section is to define and discuss the basic concepts of statistical hypothesis testing . Collectively, these concepts are sometimes referred to as the Neyman-Pearson framework, in honor of Jerzy Neyman and Egon Pearson, who first formalized them.

A statistical hypothesis is a statement about the distribution of $\bs{X}$. Equivalently, a statistical hypothesis specifies a set of possible distributions of $\bs{X}$: the set of distributions for which the statement is true. A hypothesis that specifies a single distribution for $\bs{X}$ is called simple ; a hypothesis that specifies more than one distribution for $\bs{X}$ is called composite .

In hypothesis testing , the goal is to see if there is sufficient statistical evidence to reject a presumed null hypothesis in favor of a conjectured alternative hypothesis . The null hypothesis is usually denoted $H_0$ while the alternative hypothesis is usually denoted $H_1$.

An hypothesis test is a statistical decision ; the conclusion will either be to reject the null hypothesis in favor of the alternative, or to fail to reject the null hypothesis. The decision that we make must, of course, be based on the observed value $\bs{x}$ of the data vector $\bs{X}$. Thus, we will find an appropriate subset $R$ of the sample space $S$ and reject $H_0$ if and only if $\bs{x} \in R$. The set $R$ is known as the rejection region or the critical region . Note the asymmetry between the null and alternative hypotheses. This asymmetry is due to the fact that we assume the null hypothesis, in a sense, and then see if there is sufficient evidence in $\bs{x}$ to overturn this assumption in favor of the alternative.

An hypothesis test is a statistical analogy to proof by contradiction, in a sense. Suppose for a moment that $H_1$ is a statement in a mathematical theory and that $H_0$ is its negation. One way that we can prove $H_1$ is to assume $H_0$ and work our way logically to a contradiction. In an hypothesis test, we don't prove anything of course, but there are similarities. We assume $H_0$ and then see if the data $\bs{x}$ are sufficiently at odds with that assumption that we feel justified in rejecting $H_0$ in favor of $H_1$.

Often, the critical region is defined in terms of a statistic $w(\bs{X})$, known as a test statistic , where $w$ is a function from $S$ into another set $T$. We find an appropriate rejection region $R_T \subseteq T$ and reject $H_0$ when the observed value $w(\bs{x}) \in R_T$. Thus, the rejection region in $S$ is then $R = w^{-1}(R_T) = \left\{\bs{x} \in S: w(\bs{x}) \in R_T\right\}$. As usual, the use of a statistic often allows significant data reduction when the dimension of the test statistic is much smaller than the dimension of the data vector.

The ultimate decision may be correct or may be in error. There are two types of errors, depending on which of the hypotheses is actually true.

Types of errors:

A type 1 error is rejecting the null hypothesis $H_0$ when $H_0$ is true.
A type 2 error is failing to reject the null hypothesis $H_0$ when the alternative hypothesis $H_1$ is true.

Similarly, there are two ways to make a correct decision: we could reject $H_0$ when $H_1$ is true or we could fail to reject $H_0$ when $H_0$ is true. The possibilities are summarized in the following table:

Of course, when we observe $\bs{X} = \bs{x}$ and make our decision, either we will have made the correct decision or we will have committed an error, and usually we will never know which of these events has occurred. Prior to gathering the data, however, we can consider the probabilities of the various errors.

If $H_0$ is true (that is, the distribution of $\bs{X}$ is specified by $H_0$), then $\P(\bs{X} \in R)$ is the probability of a type 1 error for this distribution. If $H_0$ is composite, then $H_0$ specifies a variety of different distributions for $\bs{X}$ and thus there is a set of type 1 error probabilities.

The maximum probability of a type 1 error, over the set of distributions specified by $ H_0 $, is the significance level of the test or the size of the critical region.

The significance level is often denoted by $\alpha$. Usually, the rejection region is constructed so that the significance level is a prescribed, small value (typically 0.1, 0.05, 0.01).

If $H_1$ is true (that is, the distribution of $\bs{X}$ is specified by $H_1$), then $\P(\bs{X} \notin R)$ is the probability of a type 2 error for this distribution. Again, if $H_1$ is composite then $H_1$ specifies a variety of different distributions for $\bs{X}$, and thus there will be a set of type 2 error probabilities. Generally, there is a tradeoff between the type 1 and type 2 error probabilities. If we reduce the probability of a type 1 error, by making the rejection region $R$ smaller, we necessarily increase the probability of a type 2 error because the complementary region $S \setminus R$ is larger.

The extreme cases can give us some insight. First consider the decision rule in which we never reject $H_0$, regardless of the evidence $\bs{x}$. This corresponds to the rejection region $R = \emptyset$. A type 1 error is impossible, so the significance level is 0. On the other hand, the probability of a type 2 error is 1 for any distribution defined by $H_1$. At the other extreme, consider the decision rule in which we always rejects $H_0$ regardless of the evidence $\bs{x}$. This corresponds to the rejection region $R = S$. A type 2 error is impossible, but now the probability of a type 1 error is 1 for any distribution defined by $H_0$. In between these two worthless tests are meaningful tests that take the evidence $\bs{x}$ into account.

If $H_1$ is true, so that the distribution of $\bs{X}$ is specified by $H_1$, then $\P(\bs{X} \in R)$, the probability of rejecting $H_0$ is the power of the test for that distribution.

Thus the power of the test for a distribution specified by $ H_1 $ is the probability of making the correct decision.

Suppose that we have two tests, corresponding to rejection regions $R_1$ and $R_2$, respectively, each having significance level $\alpha$. The test with region $R_1$ is uniformly more powerful than the test with region $R_2$ if \[ \P(\bs{X} \in R_1) \ge \P(\bs{X} \in R_2) \text{ for every distribution of } \bs{X} \text{ specified by } H_1 \]

Naturally, in this case, we would prefer the first test. Often, however, two tests will not be uniformly ordered; one test will be more powerful for some distributions specified by $H_1$ while the other test will be more powerful for other distributions specified by $H_1$.

If a test has significance level $\alpha$ and is uniformly more powerful than any other test with significance level $\alpha$, then the test is said to be a uniformly most powerful test at level $\alpha$.

Clearly a uniformly most powerful test is the best we can do.

$P$-value

In most cases, we have a general procedure that allows us to construct a test (that is, a rejection region $R_\alpha$) for any given significance level $\alpha \in (0, 1)$. Typically, $R_\alpha$ decreases (in the subset sense) as $\alpha$ decreases.

The $P$-value of the observed value $\bs{x}$ of $\bs{X}$, denoted $P(\bs{x})$, is defined to be the smallest $\alpha$ for which $\bs{x} \in R_\alpha$; that is, the smallest significance level for which $H_0$ is rejected, given $\bs{X} = \bs{x}$.

Knowing $P(\bs{x})$ allows us to test $H_0$ at any significance level for the given data $\bs{x}$: If $P(\bs{x}) \le \alpha$ then we would reject $H_0$ at significance level $\alpha$; if $P(\bs{x}) \gt \alpha$ then we fail to reject $H_0$ at significance level $\alpha$. Note that $P(\bs{X})$ is a statistic . Informally, $P(\bs{x})$ can often be thought of as the probability of an outcome as or more extreme than the observed value $\bs{x}$, where extreme is interpreted relative to the null hypothesis $H_0$.

Analogy with Justice Systems

There is a helpful analogy between statistical hypothesis testing and the criminal justice system in the US and various other countries. Consider a person charged with a crime. The presumed null hypothesis is that the person is innocent of the crime; the conjectured alternative hypothesis is that the person is guilty of the crime. The test of the hypotheses is a trial with evidence presented by both sides playing the role of the data. After considering the evidence, the jury delivers the decision as either not guilty or guilty . Note that innocent is not a possible verdict of the jury, because it is not the point of the trial to prove the person innocent. Rather, the point of the trial is to see whether there is sufficient evidence to overturn the null hypothesis that the person is innocent in favor of the alternative hypothesis of that the person is guilty. A type 1 error is convicting a person who is innocent; a type 2 error is acquitting a person who is guilty. Generally, a type 1 error is considered the more serious of the two possible errors, so in an attempt to hold the chance of a type 1 error to a very low level, the standard for conviction in serious criminal cases is beyond a reasonable doubt .

Tests of an Unknown Parameter

Hypothesis testing is a very general concept, but an important special class occurs when the distribution of the data variable $\bs{X}$ depends on a parameter $\theta$ taking values in a parameter space $\Theta$. The parameter may be vector-valued, so that $\bs{\theta} = (\theta_1, \theta_2, \ldots, \theta_n)$ and $\Theta \subseteq \R^k$ for some $k \in \N_+$. The hypotheses generally take the form \[ H_0: \theta \in \Theta_0 \text{ versus } H_1: \theta \notin \Theta_0 \] where $\Theta_0$ is a prescribed subset of the parameter space $\Theta$. In this setting, the probabilities of making an error or a correct decision depend on the true value of $\theta$. If $R$ is the rejection region, then the power function $ Q $ is given by \[ Q(\theta) = \P_\theta(\bs{X} \in R), \quad \theta \in \Theta \] The power function gives a lot of information about the test.

The power function satisfies the following properties:

$Q(\theta)$ is the probability of a type 1 error when $\theta \in \Theta_0$.
$\max\left\{Q(\theta): \theta \in \Theta_0\right\}$ is the significance level of the test.
$1 - Q(\theta)$ is the probability of a type 2 error when $\theta \notin \Theta_0$.
$Q(\theta)$ is the power of the test when $\theta \notin \Theta_0$.

If we have two tests, we can compare them by means of their power functions.

Suppose that we have two tests, corresponding to rejection regions $R_1$ and $R_2$, respectively, each having significance level $\alpha$. The test with rejection region $R_1$ is uniformly more powerful than the test with rejection region $R_2$ if $ Q_1(\theta) \ge Q_2(\theta)$ for all $ \theta \notin \Theta_0 $.

Most hypothesis tests of an unknown real parameter $\theta$ fall into three special cases:

Suppose that $ \theta $ is a real parameter and $ \theta_0 \in \Theta $ a specified value. The tests below are respectively the two-sided test , the left-tailed test , and the right-tailed test .

$H_0: \theta = \theta_0$ versus $H_1: \theta \ne \theta_0$
$H_0: \theta \ge \theta_0$ versus $H_1: \theta \lt \theta_0$
$H_0: \theta \le \theta_0$ versus $H_1: \theta \gt \theta_0$

Thus the tests are named after the conjectured alternative. Of course, there may be other unknown parameters besides $\theta$ (known as nuisance parameters ).

Equivalence Between Hypothesis Test and Confidence Sets

There is an equivalence between hypothesis tests and confidence sets for a parameter $\theta$.

Suppose that $C(\bs{x})$ is a $1 - \alpha$ level confidence set for $\theta$. The following test has significance level $\alpha$ for the hypothesis $ H_0: \theta = \theta_0 $ versus $ H_1: \theta \ne \theta_0 $: Reject $H_0$ if and only if $\theta_0 \notin C(\bs{x})$

By definition, $\P[\theta \in C(\bs{X})] = 1 - \alpha$. Hence if $H_0$ is true so that $\theta = \theta_0$, then the probability of a type 1 error is $P[\theta \notin C(\bs{X})] = \alpha$.

Equivalently, we fail to reject $H_0$ at significance level $\alpha$ if and only if $\theta_0$ is in the corresponding $1 - \alpha$ level confidence set. In particular, this equivalence applies to interval estimates of a real parameter $\theta$ and the common tests for $\theta$ given above .

In each case below, the confidence interval has confidence level $1 - \alpha$ and the test has significance level $\alpha$.

Suppose that $\left[L(\bs{X}, U(\bs{X})\right]$ is a two-sided confidence interval for $\theta$. Reject $H_0: \theta = \theta_0$ versus $H_1: \theta \ne \theta_0$ if and only if $\theta_0 \lt L(\bs{X})$ or $\theta_0 \gt U(\bs{X})$.
Suppose that $L(\bs{X})$ is a confidence lower bound for $\theta$. Reject $H_0: \theta \le \theta_0$ versus $H_1: \theta \gt \theta_0$ if and only if $\theta_0 \lt L(\bs{X})$.
Suppose that $U(\bs{X})$ is a confidence upper bound for $\theta$. Reject $H_0: \theta \ge \theta_0$ versus $H_1: \theta \lt \theta_0$ if and only if $\theta_0 \gt U(\bs{X})$.

Pivot Variables and Test Statistics

Recall that confidence sets of an unknown parameter $\theta$ are often constructed through a pivot variable , that is, a random variable $W(\bs{X}, \theta)$ that depends on the data vector $\bs{X}$ and the parameter $\theta$, but whose distribution does not depend on $\theta$ and is known. In this case, a natural test statistic for the basic tests given above is $W(\bs{X}, \theta_0)$.

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How to Write a Great Hypothesis

Hypothesis Definition, Format, Examples, and Tips

Kendra Cherry, MS, is a psychosocial rehabilitation specialist, psychology educator, and author of the "Everything Psychology Book."

Amy Morin, LCSW, is a psychotherapist and international bestselling author. Her books, including "13 Things Mentally Strong People Don't Do," have been translated into more than 40 languages. Her TEDx talk, "The Secret of Becoming Mentally Strong," is one of the most viewed talks of all time.

Verywell / Alex Dos Diaz

The Scientific Method

Hypothesis Format

Falsifiability of a hypothesis.

Operationalization

Hypothesis Types

Hypotheses examples.

Collecting Data

A hypothesis is a tentative statement about the relationship between two or more variables. It is a specific, testable prediction about what you expect to happen in a study. It is a preliminary answer to your question that helps guide the research process.

Consider a study designed to examine the relationship between sleep deprivation and test performance. The hypothesis might be: "This study is designed to assess the hypothesis that sleep-deprived people will perform worse on a test than individuals who are not sleep-deprived."

At a Glance

A hypothesis is crucial to scientific research because it offers a clear direction for what the researchers are looking to find. This allows them to design experiments to test their predictions and add to our scientific knowledge about the world. This article explores how a hypothesis is used in psychology research, how to write a good hypothesis, and the different types of hypotheses you might use.

The Hypothesis in the Scientific Method

In the scientific method , whether it involves research in psychology, biology, or some other area, a hypothesis represents what the researchers think will happen in an experiment. The scientific method involves the following steps:

Forming a question
Performing background research
Creating a hypothesis
Designing an experiment
Collecting data
Analyzing the results
Drawing conclusions
Communicating the results

The hypothesis is a prediction, but it involves more than a guess. Most of the time, the hypothesis begins with a question which is then explored through background research. At this point, researchers then begin to develop a testable hypothesis.

Unless you are creating an exploratory study, your hypothesis should always explain what you expect to happen.

In a study exploring the effects of a particular drug, the hypothesis might be that researchers expect the drug to have some type of effect on the symptoms of a specific illness. In psychology, the hypothesis might focus on how a certain aspect of the environment might influence a particular behavior.

Remember, a hypothesis does not have to be correct. While the hypothesis predicts what the researchers expect to see, the goal of the research is to determine whether this guess is right or wrong. When conducting an experiment, researchers might explore numerous factors to determine which ones might contribute to the ultimate outcome.

In many cases, researchers may find that the results of an experiment do not support the original hypothesis. When writing up these results, the researchers might suggest other options that should be explored in future studies.

In many cases, researchers might draw a hypothesis from a specific theory or build on previous research. For example, prior research has shown that stress can impact the immune system. So a researcher might hypothesize: "People with high-stress levels will be more likely to contract a common cold after being exposed to the virus than people who have low-stress levels."

In other instances, researchers might look at commonly held beliefs or folk wisdom. "Birds of a feather flock together" is one example of folk adage that a psychologist might try to investigate. The researcher might pose a specific hypothesis that "People tend to select romantic partners who are similar to them in interests and educational level."

Elements of a Good Hypothesis

So how do you write a good hypothesis? When trying to come up with a hypothesis for your research or experiments, ask yourself the following questions:

Is your hypothesis based on your research on a topic?
Can your hypothesis be tested?
Does your hypothesis include independent and dependent variables?

Before you come up with a specific hypothesis, spend some time doing background research. Once you have completed a literature review, start thinking about potential questions you still have. Pay attention to the discussion section in the journal articles you read . Many authors will suggest questions that still need to be explored.

How to Formulate a Good Hypothesis

To form a hypothesis, you should take these steps:

Collect as many observations about a topic or problem as you can.
Evaluate these observations and look for possible causes of the problem.
Create a list of possible explanations that you might want to explore.
After you have developed some possible hypotheses, think of ways that you could confirm or disprove each hypothesis through experimentation. This is known as falsifiability.

In the scientific method , falsifiability is an important part of any valid hypothesis. In order to test a claim scientifically, it must be possible that the claim could be proven false.

Students sometimes confuse the idea of falsifiability with the idea that it means that something is false, which is not the case. What falsifiability means is that if something was false, then it is possible to demonstrate that it is false.

One of the hallmarks of pseudoscience is that it makes claims that cannot be refuted or proven false.

The Importance of Operational Definitions

A variable is a factor or element that can be changed and manipulated in ways that are observable and measurable. However, the researcher must also define how the variable will be manipulated and measured in the study.

Operational definitions are specific definitions for all relevant factors in a study. This process helps make vague or ambiguous concepts detailed and measurable.

For example, a researcher might operationally define the variable " test anxiety " as the results of a self-report measure of anxiety experienced during an exam. A "study habits" variable might be defined by the amount of studying that actually occurs as measured by time.

These precise descriptions are important because many things can be measured in various ways. Clearly defining these variables and how they are measured helps ensure that other researchers can replicate your results.

Replicability

One of the basic principles of any type of scientific research is that the results must be replicable.

Replication means repeating an experiment in the same way to produce the same results. By clearly detailing the specifics of how the variables were measured and manipulated, other researchers can better understand the results and repeat the study if needed.

Some variables are more difficult than others to define. For example, how would you operationally define a variable such as aggression ? For obvious ethical reasons, researchers cannot create a situation in which a person behaves aggressively toward others.

To measure this variable, the researcher must devise a measurement that assesses aggressive behavior without harming others. The researcher might utilize a simulated task to measure aggressiveness in this situation.

Hypothesis Checklist

Does your hypothesis focus on something that you can actually test?
Does your hypothesis include both an independent and dependent variable?
Can you manipulate the variables?
Can your hypothesis be tested without violating ethical standards?

The hypothesis you use will depend on what you are investigating and hoping to find. Some of the main types of hypotheses that you might use include:

Simple hypothesis : This type of hypothesis suggests there is a relationship between one independent variable and one dependent variable.
Complex hypothesis : This type suggests a relationship between three or more variables, such as two independent and dependent variables.
Null hypothesis : This hypothesis suggests no relationship exists between two or more variables.
Alternative hypothesis : This hypothesis states the opposite of the null hypothesis.
Statistical hypothesis : This hypothesis uses statistical analysis to evaluate a representative population sample and then generalizes the findings to the larger group.
Logical hypothesis : This hypothesis assumes a relationship between variables without collecting data or evidence.

A hypothesis often follows a basic format of "If {this happens} then {this will happen}." One way to structure your hypothesis is to describe what will happen to the dependent variable if you change the independent variable .

The basic format might be: "If {these changes are made to a certain independent variable}, then we will observe {a change in a specific dependent variable}."

A few examples of simple hypotheses:

"Students who eat breakfast will perform better on a math exam than students who do not eat breakfast."
"Students who experience test anxiety before an English exam will get lower scores than students who do not experience test anxiety."
"Motorists who talk on the phone while driving will be more likely to make errors on a driving course than those who do not talk on the phone."
"Children who receive a new reading intervention will have higher reading scores than students who do not receive the intervention."

Examples of a complex hypothesis include:

"People with high-sugar diets and sedentary activity levels are more likely to develop depression."
"Younger people who are regularly exposed to green, outdoor areas have better subjective well-being than older adults who have limited exposure to green spaces."

Examples of a null hypothesis include:

"There is no difference in anxiety levels between people who take St. John's wort supplements and those who do not."
"There is no difference in scores on a memory recall task between children and adults."
"There is no difference in aggression levels between children who play first-person shooter games and those who do not."

Examples of an alternative hypothesis:

"People who take St. John's wort supplements will have less anxiety than those who do not."
"Adults will perform better on a memory task than children."
"Children who play first-person shooter games will show higher levels of aggression than children who do not."

Collecting Data on Your Hypothesis

Once a researcher has formed a testable hypothesis, the next step is to select a research design and start collecting data. The research method depends largely on exactly what they are studying. There are two basic types of research methods: descriptive research and experimental research.

Descriptive Research Methods

Descriptive research such as case studies , naturalistic observations , and surveys are often used when conducting an experiment is difficult or impossible. These methods are best used to describe different aspects of a behavior or psychological phenomenon.

Once a researcher has collected data using descriptive methods, a correlational study can examine how the variables are related. This research method might be used to investigate a hypothesis that is difficult to test experimentally.

Experimental Research Methods

Experimental methods are used to demonstrate causal relationships between variables. In an experiment, the researcher systematically manipulates a variable of interest (known as the independent variable) and measures the effect on another variable (known as the dependent variable).

Unlike correlational studies, which can only be used to determine if there is a relationship between two variables, experimental methods can be used to determine the actual nature of the relationship—whether changes in one variable actually cause another to change.

The hypothesis is a critical part of any scientific exploration. It represents what researchers expect to find in a study or experiment. In situations where the hypothesis is unsupported by the research, the research still has value. Such research helps us better understand how different aspects of the natural world relate to one another. It also helps us develop new hypotheses that can then be tested in the future.

Thompson WH, Skau S. On the scope of scientific hypotheses . R Soc Open Sci . 2023;10(8):230607. doi:10.1098/rsos.230607

Taran S, Adhikari NKJ, Fan E. Falsifiability in medicine: what clinicians can learn from Karl Popper [published correction appears in Intensive Care Med. 2021 Jun 17;:]. Intensive Care Med . 2021;47(9):1054-1056. doi:10.1007/s00134-021-06432-z

Eyler AA. Research Methods for Public Health . 1st ed. Springer Publishing Company; 2020. doi:10.1891/9780826182067.0004

Nosek BA, Errington TM. What is replication ? PLoS Biol . 2020;18(3):e3000691. doi:10.1371/journal.pbio.3000691

Aggarwal R, Ranganathan P. Study designs: Part 2 - Descriptive studies . Perspect Clin Res . 2019;10(1):34-36. doi:10.4103/picr.PICR_154_18

Nevid J. Psychology: Concepts and Applications. Wadworth, 2013.

By Kendra Cherry, MSEd Kendra Cherry, MS, is a psychosocial rehabilitation specialist, psychology educator, and author of the "Everything Psychology Book."

What Is A Research (Scientific) Hypothesis? A plain-language explainer + examples

By: Derek Jansen (MBA) | Reviewed By: Dr Eunice Rautenbach | June 2020

If you’re new to the world of research, or it’s your first time writing a dissertation or thesis, you’re probably noticing that the words “research hypothesis” and “scientific hypothesis” are used quite a bit, and you’re wondering what they mean in a research context .

“Hypothesis” is one of those words that people use loosely, thinking they understand what it means. However, it has a very specific meaning within academic research. So, it’s important to understand the exact meaning before you start hypothesizing.

Research Hypothesis 101

What is a hypothesis ?
What is a research hypothesis (scientific hypothesis)?
Requirements for a research hypothesis
Definition of a research hypothesis
The null hypothesis

What is a hypothesis?

Let’s start with the general definition of a hypothesis (not a research hypothesis or scientific hypothesis), according to the Cambridge Dictionary:

Hypothesis: an idea or explanation for something that is based on known facts but has not yet been proved.

In other words, it’s a statement that provides an explanation for why or how something works, based on facts (or some reasonable assumptions), but that has not yet been specifically tested . For example, a hypothesis might look something like this:

Hypothesis: sleep impacts academic performance.

This statement predicts that academic performance will be influenced by the amount and/or quality of sleep a student engages in – sounds reasonable, right? It’s based on reasonable assumptions , underpinned by what we currently know about sleep and health (from the existing literature). So, loosely speaking, we could call it a hypothesis, at least by the dictionary definition.

But that’s not good enough…

Unfortunately, that’s not quite sophisticated enough to describe a research hypothesis (also sometimes called a scientific hypothesis), and it wouldn’t be acceptable in a dissertation, thesis or research paper . In the world of academic research, a statement needs a few more criteria to constitute a true research hypothesis .

What is a research hypothesis?

A research hypothesis (also called a scientific hypothesis) is a statement about the expected outcome of a study (for example, a dissertation or thesis). To constitute a quality hypothesis, the statement needs to have three attributes – specificity , clarity and testability .

Let’s take a look at these more closely.

Need a helping hand?

Hypothesis Essential #1: Specificity & Clarity

A good research hypothesis needs to be extremely clear and articulate about both what’ s being assessed (who or what variables are involved ) and the expected outcome (for example, a difference between groups, a relationship between variables, etc.).

Let’s stick with our sleepy students example and look at how this statement could be more specific and clear.

Hypothesis: Students who sleep at least 8 hours per night will, on average, achieve higher grades in standardised tests than students who sleep less than 8 hours a night.

As you can see, the statement is very specific as it identifies the variables involved (sleep hours and test grades), the parties involved (two groups of students), as well as the predicted relationship type (a positive relationship). There’s no ambiguity or uncertainty about who or what is involved in the statement, and the expected outcome is clear.

Contrast that to the original hypothesis we looked at – “Sleep impacts academic performance” – and you can see the difference. “Sleep” and “academic performance” are both comparatively vague , and there’s no indication of what the expected relationship direction is (more sleep or less sleep). As you can see, specificity and clarity are key.

A good research hypothesis needs to be very clear about what’s being assessed and very specific about the expected outcome.

Hypothesis Essential #2: Testability (Provability)

A statement must be testable to qualify as a research hypothesis. In other words, there needs to be a way to prove (or disprove) the statement. If it’s not testable, it’s not a hypothesis – simple as that.

For example, consider the hypothesis we mentioned earlier:

Hypothesis: Students who sleep at least 8 hours per night will, on average, achieve higher grades in standardised tests than students who sleep less than 8 hours a night.

We could test this statement by undertaking a quantitative study involving two groups of students, one that gets 8 or more hours of sleep per night for a fixed period, and one that gets less. We could then compare the standardised test results for both groups to see if there’s a statistically significant difference.

Again, if you compare this to the original hypothesis we looked at – “Sleep impacts academic performance” – you can see that it would be quite difficult to test that statement, primarily because it isn’t specific enough. How much sleep? By who? What type of academic performance?

So, remember the mantra – if you can’t test it, it’s not a hypothesis 🙂

A good research hypothesis must be testable. In other words, you must able to collect observable data in a scientifically rigorous fashion to test it.

Defining A Research Hypothesis

You’re still with us? Great! Let’s recap and pin down a clear definition of a hypothesis.

A research hypothesis (or scientific hypothesis) is a statement about an expected relationship between variables, or explanation of an occurrence, that is clear, specific and testable.

So, when you write up hypotheses for your dissertation or thesis, make sure that they meet all these criteria. If you do, you’ll not only have rock-solid hypotheses but you’ll also ensure a clear focus for your entire research project.

What about the null hypothesis?

You may have also heard the terms null hypothesis , alternative hypothesis, or H-zero thrown around. At a simple level, the null hypothesis is the counter-proposal to the original hypothesis.

For example, if the hypothesis predicts that there is a relationship between two variables (for example, sleep and academic performance), the null hypothesis would predict that there is no relationship between those variables.

At a more technical level, the null hypothesis proposes that no statistical significance exists in a set of given observations and that any differences are due to chance alone.

And there you have it – hypotheses in a nutshell.

If you have any questions, be sure to leave a comment below and we’ll do our best to help you. If you need hands-on help developing and testing your hypotheses, consider our private coaching service , where we hold your hand through the research journey.

Psst... there’s more!

This post was based on one of our popular Research Bootcamps . If you're working on a research project, you'll definitely want to check this out ...

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16 Comments

Very useful information. I benefit more from getting more information in this regard.

Very great insight,educative and informative. Please give meet deep critics on many research data of public international Law like human rights, environment, natural resources, law of the sea etc

In a book I read a distinction is made between null, research, and alternative hypothesis. As far as I understand, alternative and research hypotheses are the same. Can you please elaborate? Best Afshin

This is a self explanatory, easy going site. I will recommend this to my friends and colleagues.

Very good definition. How can I cite your definition in my thesis? Thank you. Is nul hypothesis compulsory in a research?

It’s a counter-proposal to be proven as a rejection

Please what is the difference between alternate hypothesis and research hypothesis?

It is a very good explanation. However, it limits hypotheses to statistically tasteable ideas. What about for qualitative researches or other researches that involve quantitative data that don’t need statistical tests?

In qualitative research, one typically uses propositions, not hypotheses.

could you please elaborate it more

I’ve benefited greatly from these notes, thank you.

This is very helpful

well articulated ideas are presented here, thank you for being reliable sources of information

Excellent. Thanks for being clear and sound about the research methodology and hypothesis (quantitative research)

I have only a simple question regarding the null hypothesis. – Is the null hypothesis (Ho) known as the reversible hypothesis of the alternative hypothesis (H1? – How to test it in academic research?

this is very important note help me much more

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Hypothesis: Functions, Problems, Types, Characteristics, Examples

Basic Elements of the Scientific Method: Hypotheses

The Function of the Hypotheses

A hypothesis states what one is looking for in an experiment. When facts are assembled, ordered, and seen in a relationship, they build up to become a theory. This theory needs to be deduced for further confirmation of the facts, this formulation of the deductions constitutes of a hypothesis. As a theory states a logical relationship between facts and from this, the propositions which are deduced should be true. Hence, these deduced prepositions are called hypotheses.

Problems in Formulating the Hypothesis

As difficult as the process may be, it is very essential to understand the need of a hypothesis. The research would be much unfocused and a random empirical wandering without it. The hypothesis provides a necessary link between the theory and investigation which often leads to the discovery of additions to knowledge.

There are three major difficulties in the formulation of a hypothesis, they are as follows:

Absence of a clear theoretical framework
Lack of ability to utilize that theoretical framework logically
Failure to be acquainted with available research techniques so as to phrase the hypothesis properly.

Sometimes the deduction of a hypothesis may be difficult as there would be many variables and the necessity to take them all into consideration becomes a challenge. For instance, observing two cases:

Principle: A socially recognized relationship with built-in strains also governed by the institutional controls has to ensure conformity of the participants with implicit or explicit norms.

Deduction: This situation holds much more sense to the people who are in professions such as psychotherapy, psychiatry and law to some extent. They possess a very intimate relationship with their clients, thus are more susceptible to issues regarding emotional strains in the client-practitioner relationship and more implicit and explicit controls over both participants in comparison to other professions.

The above-mentioned case has variable hypotheses, so the need is to break them down into sub hypotheses, they are as follows:

Specification of the degree of difference
Specification of profession and problem
Specification of kinds of controls.

2. Principle: Extensive but relatively systematized data show the correlation between members of the upper occupational class and less unhappiness and worry. Also, they are subjected to more formal controls than members of the lower strata.

Deduction: There can numerous ways to approach this principle, one could go with the comparison applying to martial relationships of the members and further argue that such differential pressures could be observed through divorce rates. This hypothesis would show inverse correlations between class position and divorce rates. There would be a very strong need to define the terms carefully to show the deduction from the principle problem.

The reference of these examples showcases a major issue in the hypothesis formulations procedures. One needs to keep the lines set for the deductions and one should be focusing on having a hypothesis at the beginning of the experiment, that hypothesis may be subject to change in the later stages and it is referred to as a „working hypothesis. Hence, the devising and utilization of a hypothesis is essential for the success of the experiment.

Types of Hypothesis

There are many ways to classify hypotheses, but it seems adequate to distinguish to separate them on the basis of their level of abstraction. They can be divided into three broad levels which will be increasing in abstractness.

The existence of empirical uniformities : These hypotheses are made from problems which usually have a very high percentage of representing scientific examination of common–sense proportions. These studies may show a variety of things such as the distribution of business establishments in a city, behavior patterns of specific groups, etc. and they tend to show no irregularities in their data collection or review. There have been arguments which say that these aren’t hypothesis as they represent what everyone knows. This can be counter argued on the basis of two things that, “what everyone knows” isn’t always in coherence with the framework of science and it may also be incorrect. Hence, testing these hypotheses is necessary too.
Complex ideal types: These hypotheses aim at testing the existence of logically derived relationships between empirical uniformities. This can be understood with an example, to observe ecology one should take in many factors and see the relationship between and how they affect the greater issue. A theory by Ernest W. Burgess gave out the statement that concentric growth circles are the one which characterize the city. Hence, all issues such as land values, industrial growth, ethnic groups, etc. are needed to be analyzed for forming a correct and reasonable hypothesis.
Relations of analytic variables: These hypotheses are a bit more complex as they focus on they lead to the formulation of a relationship between the changes in one property with respect to another. For instance, taking the example of human fertility in diverse regions, religions, wealth gap, etc. may not always affect the end result but it doesn’t mean that the variables need not be accounted for. This level of hypothesizing is one of the most effective and sophisticated and thus is only limited by theory itself.

Science and Hypothesis

“The general culture in which a science develops furnishes many of its basic hypotheses” holds true as science has developed more in the West and is no accident that it is a function of culture itself. This is quite evident with the culture of the West as they read for morals, science and happiness. After the examination of a bunch of variables, it is quite easy to say that the cultural emphasis upon happiness has been productive of an almost limitless range.

The hypotheses originate from science; a key example in the form of “socialization” may be taken. The socialization process in learning science involves a feedback mechanism between the scientist and the student. The student learns from the scientist and then tests for results with his own experience, and the scientist in turn has to do the same with his colleagues.

Analogies are a source of useful hypotheses but not without its dangers as all variables may not be accounted for it as no civilization has a perfect system.

Hypotheses are also the consequence of personal, idiosyncratic experience as the manner in which the individual reacts to the hypotheses is also important and should be accounted for in the experiment.

The Characteristics for Usable Hypotheses

The criteria for judging a hypothesis as mentioned below:

Complete Clarity : A good hypothesis should have two main elements, the concepts should be clearly defined and they should be definitions which are communicable and accepted by a larger section of the public. A lot of sources may be used and fellow associates may be used to help with the cause.
Empirical Referents : A great hypothesis should have scientific concepts with the ultimate empirical referent. It can‟t be based on moral judgment though it can explore them but the goal should be separated from moral preachment and the acceptance of values. A good start could be analyzing the concepts which express attitudes rather than describing or referring to empirical phenomena.
Specific Goal : The goal and procedure of the hypothesis should be tangible as grand experiments are harder to carry out. All operations and predictions should be mapped and in turn the possibility of testing the hypothesis increases. This not only enables the conceptual clarity but also the description of any indexes used. These indexes are used as variables for testing hypotheses on a larger scale. A general prediction isn’t as reliable as a specific prediction as the specific prediction provides a better result.
Relation to Available Techniques : The technique with which a hypothesis is tested is of the utmost importance and so thorough research should be carried out before the experiment in order to find the best possible way to go about it. The example of Karl Marx may be given regarding his renowned theories; he formulated his hypothesis by observing individuals and thus proving his hypothesis. So, finding the right technique may be the key to a successful test.
Relation to a Body of Theory: Theories on social relations can never be developed in isolation but they are a further extension of already developed or developing theories. For instance, if the “intelligence quotient” of a member of the society is to be measured, certain variables such as caste, ethnicity, nationality, etc. are chosen thus deductions are made from time to time to eventually find out what is the factor that influences intelligence.

The Conclusion

The formulation of a hypothesis is probably the most necessary step in good research practice and it is very essential to get the thought process started. It helps the researcher to have a specific goal in mind and deduce the end result of an experiment with ease and efficiency. History is evident that asking the right questions always works out fine.

Definition of hypothesis

Did you know.

The Difference Between Hypothesis and Theory

A hypothesis is an assumption, an idea that is proposed for the sake of argument so that it can be tested to see if it might be true.

In the scientific method, the hypothesis is constructed before any applicable research has been done, apart from a basic background review. You ask a question, read up on what has been studied before, and then form a hypothesis.

A hypothesis is usually tentative; it's an assumption or suggestion made strictly for the objective of being tested.

A theory , in contrast, is a principle that has been formed as an attempt to explain things that have already been substantiated by data. It is used in the names of a number of principles accepted in the scientific community, such as the Big Bang Theory . Because of the rigors of experimentation and control, it is understood to be more likely to be true than a hypothesis is.

In non-scientific use, however, hypothesis and theory are often used interchangeably to mean simply an idea, speculation, or hunch, with theory being the more common choice.

Since this casual use does away with the distinctions upheld by the scientific community, hypothesis and theory are prone to being wrongly interpreted even when they are encountered in scientific contexts—or at least, contexts that allude to scientific study without making the critical distinction that scientists employ when weighing hypotheses and theories.

The most common occurrence is when theory is interpreted—and sometimes even gleefully seized upon—to mean something having less truth value than other scientific principles. (The word law applies to principles so firmly established that they are almost never questioned, such as the law of gravity.)

This mistake is one of projection: since we use theory in general to mean something lightly speculated, then it's implied that scientists must be talking about the same level of uncertainty when they use theory to refer to their well-tested and reasoned principles.

The distinction has come to the forefront particularly on occasions when the content of science curricula in schools has been challenged—notably, when a school board in Georgia put stickers on textbooks stating that evolution was "a theory, not a fact, regarding the origin of living things." As Kenneth R. Miller, a cell biologist at Brown University, has said , a theory "doesn’t mean a hunch or a guess. A theory is a system of explanations that ties together a whole bunch of facts. It not only explains those facts, but predicts what you ought to find from other observations and experiments.”

While theories are never completely infallible, they form the basis of scientific reasoning because, as Miller said "to the best of our ability, we’ve tested them, and they’ve held up."

proposition
supposition

hypothesis , theory , law mean a formula derived by inference from scientific data that explains a principle operating in nature.

hypothesis implies insufficient evidence to provide more than a tentative explanation.

theory implies a greater range of evidence and greater likelihood of truth.

law implies a statement of order and relation in nature that has been found to be invariable under the same conditions.

Examples of hypothesis in a Sentence

These examples are programmatically compiled from various online sources to illustrate current usage of the word 'hypothesis.' Any opinions expressed in the examples do not represent those of Merriam-Webster or its editors. Send us feedback about these examples.

Word History

Greek, from hypotithenai to put under, suppose, from hypo- + tithenai to put — more at do

1641, in the meaning defined at sense 1a

Phrases Containing hypothesis

counter - hypothesis
nebular hypothesis
null hypothesis
planetesimal hypothesis
Whorfian hypothesis

Articles Related to hypothesis

This is the Difference Between a...

This is the Difference Between a Hypothesis and a Theory

In scientific reasoning, they're two completely different things

Dictionary Entries Near hypothesis

hypothermia

hypothesize

Cite this Entry

“Hypothesis.” Merriam-Webster.com Dictionary , Merriam-Webster, https://www.merriam-webster.com/dictionary/hypothesis. Accessed 27 May. 2024.

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Hypothesis testing involves formulating assumptions about population parameters based on sample statistics and rigorously evaluating these assumptions against empirical evidence. This article sheds light on the significance of hypothesis testing and the critical steps involved in the process.

What is Hypothesis Testing?

Hypothesis testing is a statistical method that is used to make a statistical decision using experimental data. Hypothesis testing is basically an assumption that we make about a population parameter. It evaluates two mutually exclusive statements about a population to determine which statement is best supported by the sample data.

Example: You say an average height in the class is 30 or a boy is taller than a girl. All of these is an assumption that we are assuming, and we need some statistical way to prove these. We need some mathematical conclusion whatever we are assuming is true.

Defining Hypotheses

$\mu$

Key Terms of Hypothesis Testing

$\alpha$

P-value: The P value , or calculated probability, is the probability of finding the observed/extreme results when the null hypothesis(H0) of a study-given problem is true. If your P-value is less than the chosen significance level then you reject the null hypothesis i.e. accept that your sample claims to support the alternative hypothesis.
Test Statistic: The test statistic is a numerical value calculated from sample data during a hypothesis test, used to determine whether to reject the null hypothesis. It is compared to a critical value or p-value to make decisions about the statistical significance of the observed results.
Critical value : The critical value in statistics is a threshold or cutoff point used to determine whether to reject the null hypothesis in a hypothesis test.
Degrees of freedom: Degrees of freedom are associated with the variability or freedom one has in estimating a parameter. The degrees of freedom are related to the sample size and determine the shape.

Why do we use Hypothesis Testing?

Hypothesis testing is an important procedure in statistics. Hypothesis testing evaluates two mutually exclusive population statements to determine which statement is most supported by sample data. When we say that the findings are statistically significant, thanks to hypothesis testing.

One-Tailed and Two-Tailed Test

One tailed test focuses on one direction, either greater than or less than a specified value. We use a one-tailed test when there is a clear directional expectation based on prior knowledge or theory. The critical region is located on only one side of the distribution curve. If the sample falls into this critical region, the null hypothesis is rejected in favor of the alternative hypothesis.

One-Tailed Test

There are two types of one-tailed test:

$\mu \geq 50$

Two-Tailed Test

A two-tailed test considers both directions, greater than and less than a specified value.We use a two-tailed test when there is no specific directional expectation, and want to detect any significant difference.

$\mu =$

What are Type 1 and Type 2 errors in Hypothesis Testing?

In hypothesis testing, Type I and Type II errors are two possible errors that researchers can make when drawing conclusions about a population based on a sample of data. These errors are associated with the decisions made regarding the null hypothesis and the alternative hypothesis.

$\alpha$

How does Hypothesis Testing work?

Step 1: define null and alternative hypothesis.

We first identify the problem about which we want to make an assumption keeping in mind that our assumption should be contradictory to one another, assuming Normally distributed data.

Step 2 – Choose significance level

$\alpha$

Step 3 – Collect and Analyze data.

Gather relevant data through observation or experimentation. Analyze the data using appropriate statistical methods to obtain a test statistic.

Step 4-Calculate Test Statistic

The data for the tests are evaluated in this step we look for various scores based on the characteristics of data. The choice of the test statistic depends on the type of hypothesis test being conducted.

There are various hypothesis tests, each appropriate for various goal to calculate our test. This could be a Z-test , Chi-square , T-test , and so on.

Z-test : If population means and standard deviations are known. Z-statistic is commonly used.
t-test : If population standard deviations are unknown. and sample size is small than t-test statistic is more appropriate.
Chi-square test : Chi-square test is used for categorical data or for testing independence in contingency tables
F-test : F-test is often used in analysis of variance (ANOVA) to compare variances or test the equality of means across multiple groups.

We have a smaller dataset, So, T-test is more appropriate to test our hypothesis.

T-statistic is a measure of the difference between the means of two groups relative to the variability within each group. It is calculated as the difference between the sample means divided by the standard error of the difference. It is also known as the t-value or t-score.

Step 5 – Comparing Test Statistic:

In this stage, we decide where we should accept the null hypothesis or reject the null hypothesis. There are two ways to decide where we should accept or reject the null hypothesis.

Method A: Using Crtical values

Comparing the test statistic and tabulated critical value we have,

If Test Statistic>Critical Value: Reject the null hypothesis.
If Test Statistic≤Critical Value: Fail to reject the null hypothesis.

Note: Critical values are predetermined threshold values that are used to make a decision in hypothesis testing. To determine critical values for hypothesis testing, we typically refer to a statistical distribution table , such as the normal distribution or t-distribution tables based on.

Method B: Using P-values

We can also come to an conclusion using the p-value,

$p\leq\alpha$

Note : The p-value is the probability of obtaining a test statistic as extreme as, or more extreme than, the one observed in the sample, assuming the null hypothesis is true. To determine p-value for hypothesis testing, we typically refer to a statistical distribution table , such as the normal distribution or t-distribution tables based on.

Step 7- Interpret the Results

At last, we can conclude our experiment using method A or B.

Calculating test statistic

To validate our hypothesis about a population parameter we use statistical functions . We use the z-score, p-value, and level of significance(alpha) to make evidence for our hypothesis for normally distributed data .

1. Z-statistics:

When population means and standard deviations are known.

$z = \frac{\bar{x} - \mu}{\frac{\sigma}{\sqrt{n}}}$

μ represents the population mean,
σ is the standard deviation
and n is the size of the sample.

2. T-Statistics

T test is used when n<30,

t-statistic calculation is given by:

$t=\frac{x̄-μ}{s/\sqrt{n}}$

t = t-score,
x̄ = sample mean
μ = population mean,
s = standard deviation of the sample,
n = sample size

3. Chi-Square Test

Chi-Square Test for Independence categorical Data (Non-normally distributed) using:

$\chi^2 = \sum \frac{(O_{ij} - E_{ij})^2}{E_{ij}}$

i,j are the rows and columns index respectively.

$E_{ij}$

Real life Hypothesis Testing example

Let’s examine hypothesis testing using two real life situations,

Case A: D oes a New Drug Affect Blood Pressure?

Imagine a pharmaceutical company has developed a new drug that they believe can effectively lower blood pressure in patients with hypertension. Before bringing the drug to market, they need to conduct a study to assess its impact on blood pressure.

Before Treatment: 120, 122, 118, 130, 125, 128, 115, 121, 123, 119
After Treatment: 115, 120, 112, 128, 122, 125, 110, 117, 119, 114

Step 1 : Define the Hypothesis

Null Hypothesis : (H 0 )The new drug has no effect on blood pressure.
Alternate Hypothesis : (H 1 )The new drug has an effect on blood pressure.

Step 2: Define the Significance level

Let’s consider the Significance level at 0.05, indicating rejection of the null hypothesis.

If the evidence suggests less than a 5% chance of observing the results due to random variation.

Step 3 : Compute the test statistic

Using paired T-test analyze the data to obtain a test statistic and a p-value.

The test statistic (e.g., T-statistic) is calculated based on the differences between blood pressure measurements before and after treatment.

t = m/(s/√n)

m = mean of the difference i.e X after, X before
s = standard deviation of the difference (d) i.e d i = X after, i − X before,
n = sample size,

then, m= -3.9, s= 1.8 and n= 10

we, calculate the , T-statistic = -9 based on the formula for paired t test

Step 4: Find the p-value

The calculated t-statistic is -9 and degrees of freedom df = 9, you can find the p-value using statistical software or a t-distribution table.

thus, p-value = 8.538051223166285e-06

Step 5: Result

If the p-value is less than or equal to 0.05, the researchers reject the null hypothesis.
If the p-value is greater than 0.05, they fail to reject the null hypothesis.

Conclusion: Since the p-value (8.538051223166285e-06) is less than the significance level (0.05), the researchers reject the null hypothesis. There is statistically significant evidence that the average blood pressure before and after treatment with the new drug is different.

Python Implementation of Hypothesis Testing

Let’s create hypothesis testing with python, where we are testing whether a new drug affects blood pressure. For this example, we will use a paired T-test. We’ll use the scipy.stats library for the T-test.

Scipy is a mathematical library in Python that is mostly used for mathematical equations and computations.

We will implement our first real life problem via python,

In the above example, given the T-statistic of approximately -9 and an extremely small p-value, the results indicate a strong case to reject the null hypothesis at a significance level of 0.05.

The results suggest that the new drug, treatment, or intervention has a significant effect on lowering blood pressure.
The negative T-statistic indicates that the mean blood pressure after treatment is significantly lower than the assumed population mean before treatment.

Case B : Cholesterol level in a population

Data: A sample of 25 individuals is taken, and their cholesterol levels are measured.

Cholesterol Levels (mg/dL): 205, 198, 210, 190, 215, 205, 200, 192, 198, 205, 198, 202, 208, 200, 205, 198, 205, 210, 192, 205, 198, 205, 210, 192, 205.

Populations Mean = 200

Population Standard Deviation (σ): 5 mg/dL(given for this problem)

Step 1: Define the Hypothesis

Null Hypothesis (H 0 ): The average cholesterol level in a population is 200 mg/dL.
Alternate Hypothesis (H 1 ): The average cholesterol level in a population is different from 200 mg/dL.

As the direction of deviation is not given , we assume a two-tailed test, and based on a normal distribution table, the critical values for a significance level of 0.05 (two-tailed) can be calculated through the z-table and are approximately -1.96 and 1.96.

$(203.8 - 200) / (5 \div \sqrt{25})$

Step 4: Result

Since the absolute value of the test statistic (2.04) is greater than the critical value (1.96), we reject the null hypothesis. And conclude that, there is statistically significant evidence that the average cholesterol level in the population is different from 200 mg/dL

Limitations of Hypothesis Testing

Although a useful technique, hypothesis testing does not offer a comprehensive grasp of the topic being studied. Without fully reflecting the intricacy or whole context of the phenomena, it concentrates on certain hypotheses and statistical significance.
The accuracy of hypothesis testing results is contingent on the quality of available data and the appropriateness of statistical methods used. Inaccurate data or poorly formulated hypotheses can lead to incorrect conclusions.
Relying solely on hypothesis testing may cause analysts to overlook significant patterns or relationships in the data that are not captured by the specific hypotheses being tested. This limitation underscores the importance of complimenting hypothesis testing with other analytical approaches.

Hypothesis testing stands as a cornerstone in statistical analysis, enabling data scientists to navigate uncertainties and draw credible inferences from sample data. By systematically defining null and alternative hypotheses, choosing significance levels, and leveraging statistical tests, researchers can assess the validity of their assumptions. The article also elucidates the critical distinction between Type I and Type II errors, providing a comprehensive understanding of the nuanced decision-making process inherent in hypothesis testing. The real-life example of testing a new drug’s effect on blood pressure using a paired T-test showcases the practical application of these principles, underscoring the importance of statistical rigor in data-driven decision-making.

Frequently Asked Questions (FAQs)

1. what are the 3 types of hypothesis test.

There are three types of hypothesis tests: right-tailed, left-tailed, and two-tailed. Right-tailed tests assess if a parameter is greater, left-tailed if lesser. Two-tailed tests check for non-directional differences, greater or lesser.

2.What are the 4 components of hypothesis testing?

Null Hypothesis ( ): No effect or difference exists. Alternative Hypothesis ( ): An effect or difference exists. Significance Level ( ): Risk of rejecting null hypothesis when it’s true (Type I error). Test Statistic: Numerical value representing observed evidence against null hypothesis.

3.What is hypothesis testing in ML?

Statistical method to evaluate the performance and validity of machine learning models. Tests specific hypotheses about model behavior, like whether features influence predictions or if a model generalizes well to unseen data.

4.What is the difference between Pytest and hypothesis in Python?

Pytest purposes general testing framework for Python code while Hypothesis is a Property-based testing framework for Python, focusing on generating test cases based on specified properties of the code.

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Open access
Published: 23 May 2024

Performance in myoelectric pattern recognition improves with transcranial direct current stimulation

Shahrzad Damercheli 1 , 2 ,
Kelly Morrenhof 1 , 2 ,
Kirstin Ahmed 1 , 2 &
Max Ortiz-Catalan ORCID: orcid.org/0000-0002-6084-3865 1 , 2 , 3 , 4 , 5 , 6

Scientific Reports volume 14 , Article number: 11744 ( 2024 ) Cite this article

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Biomedical engineering
Electrical and electronic engineering
Translational research

Sensorimotor impairments, resulting from conditions like stroke and amputations, can profoundly impact an individual’s functional abilities and overall quality of life. Assistive and rehabilitation devices such as prostheses, exo-skeletons, and serious gaming in virtual environments can help to restore some degree of function and alleviate pain after sensorimotor impairments. Myoelectric pattern recognition (MPR) has gained popularity in the past decades as it provides superior control over said devices, and therefore efforts to facilitate and improve performance in MPR can result in better rehabilitation outcomes. One possibility to enhance MPR is to employ transcranial direct current stimulation (tDCS) to facilitate motor learning. Twelve healthy able-bodied individuals participated in this crossover study to determine the effect of tDCS on MPR performance. Baseline training was followed by two sessions of either sham or anodal tDCS using the dominant and non-dominant arms. Assignments were randomized, and the MPR task consisted of 11 different hand/wrist movements, including rest or no movement. Surface electrodes were used to record EMG and the MPR open-source platform, BioPatRec, was used for decoding motor volition in real-time. The motion test was used to evaluate performance. We hypothesized that using anodal tDCS to increase the excitability of the primary motor cortex associated with non-dominant side in able-bodied individuals, will improve motor learning and thus MPR performance. Overall, we found that tDCS enhanced MPR performance, particularly in the non-dominant side. We were able to reject the null hypothesis and improvements in the motion test’s completion rate during tDCS (28% change, p-value: 0.023) indicate its potential as an adjunctive tool to enhance MPR and motor learning. tDCS appears promising as a tool to enhance the learning phase of using assistive devices using MPR, such as myoelectric prostheses.

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Introduction.

Sensorimotor impairments, such as those arising from stroke and amputations, have significant effects on functional capabilities and quality of life. In one study (n = 94,905), 21% of stroke patients experienced motor impairment alone and in total, 82% experienced motor impairment in addition to sensory, cognitive, or sensory and cognitive impairment 1 . The most prevalent impairment following stroke is a contralateral upper limb hemiparesis affecting > 80% of patients during the acute phase and > 40% in the chronic phase 2 .

Amputations are one of the most extreme cases of sensorimotor impairments, resulting in reduced quality of life, and often unpleasant sensations and pain in the phantom limb 3 , 4 . Although there is no perfect solution for these impairments, advanced prosthetic systems can restore a certain degree of function after an amputation 5 , 6 , 7 , and phantom limb pain (PLP) can be alleviated by guided plasticity approaches such mirror therapy 8 , 9 , 10 , 11 , graded motor imagery 12 , 13 , and phantom motor execution 14 , 15 . Similar approaches have also been used for the rehabilitation of stroke patients 2 .

One method to increase prosthetic limb control is myoelectric pattern recognition (MPR) which decodes patterns of muscle activity in the residual muscles of amputated limbs 16 , 17 . Embedded systems running MPR are able to infer movement intention using machine learning algorithms to control prosthetic devices 18 . This provides a more intuitive control of the prosthetic limb compared to simpler one-to-one electromyography (EMG) strategies (one EMG signal to drive one prosthetic function). In addition, MPR has been employed for treatment of PLP in individuals with amputation 14 , 19 , and functional restoration in patients after stroke. One way to increase prosthetic control performance using MPR is using targeted rehabilitation that includes repetitive exercise and prolonged training. Research indicates that this also increases prosthetic use 20 , 21 , however, these methods are often time-consuming and can lead to user frustration and possibly abandonment of the prosthetic device.

Treatments that are used in stroke recovery include non-invasive brain stimulation techniques which modulate brain activity by introducing external stimuli 22 . Examples include transcranial electrical stimulation (tES), such as transcranial direct current stimulation (tDCS), and repetitive transcranial magnetic stimulation (rTMS). These techniques enhance or inhibit neuronal activity 23 and consequently, alter sensorimotor and cognitive functions 24 , 25 . tES changes the cell membrane potential and thus modulate the spontaneous firing rate 22 , which has been shown to increase motor learning 26 and the recovery of motor dysfunction 27 . A meta-analysis by Bai et al., concluded that tDCS is effective for stroke recovery of patients with motor dysfunction 27 . Furthermore tDCS has been used in the rehabilitation of several additional neurological disorders such as depression, anxiety, and schizophrenia 24 , 25 , 28 , as well as for the relief of PLP 29 .

In a small number of individuals with unilateral upper limb amputation, tDCS has been shown to improve the ability of the subjects to produce distinct EMG signals, which is useful for MPR applied to the control of prosthetic limbs 30 . Similarly, tDCS has shown promising results on hand performance in able-bodied individuals using the Jebsen Taylor hand function test (JTHFT). In one study, both the dominant and non-dominant primary motor cortex were targeted resulting in improved motor function in the non-dominant hand following modulation of the corresponding primary motor cortex 31 . The effect of tDCS seems to be observable in the affected or non-dominant side, however, the evidence is limited and replication by independent groups has not been conducted.

We undertake research in the field of prosthetic limbs 5 , 6 , rehabilitation of PLP 14 , 29 , and stroke 32 which would all benefit from validating the efficacy of tDCS to improve MPR. Here, we evaluated the hypothesis that tDCS improves MPR in the non-dominant hand of able-bodied individuals in a cross-over, sham-controlled study. In addition, we also assess the effect of learning and the application of tDCS in the dominant arm. We used the completion rate of the motion test 33 as a measure of the participants ability to accomplish a motor task using MPR. In addition, we also measured the time in which each task was completed (completion time), and the reliability of decoding (accuracy).

Twelve healthy able-bodied individuals conducted baseline training followed by two sessions of either sham or anodal tDCS on the primary motor cortex of the dominant and non-dominant arms, separately. Assignments were randomized, and the MPR task involved 11 different hand/wrist movements, including rest or no movement. Surface electrodes recorded EMG signals, and real-time motor volition decoding was performed using the open-source platform BioPatRec 17 . Performance evaluation was conducted using the motion test as implanted in BioPatRec.

Participants

In total, 12 able-bodied individuals participated in this study, of which six were females and six were males ranging from 23–33 (27 ± 3.6) years old. Eleven participants were right-handed, one participant was left-handed. All study participants successfully completed all sessions, demonstrating good tolerance to the intervention. Upon initiation of anodal tDCS, all participants experienced a tingling sensation underneath the anode electrode of tDCS, attributed to the electrical current passing through the skin and underlying tissues 34 . One participant reported a side effect characterized by redness underneath the cathode electrode. This was a singular occurrence and did not necessitate termination of their participation in the study.

Statistical analysis

Due to the non-normal distribution of the data (tested by Shapiro–Wilkes and Kolmogorov) and the limited sample size, the non-parametric Wilcoxon Signed ranks test (WRST) (p-value < 0.05, two-tailed) was selected to investigate the significance of tDCS on completion rate, completion time, and accuracy (Table 1 ). The null hypothesis for this study was that there would be no significant changes in the completion rate before and after tDCS application. The analysis was conducted on data collected within each session, including baseline, sham stimulation, and active stimulation. Additionally, similar analyses were carried out for sub-groups based on dominant and non-dominant sides. Furthermore, we found no statistically significant differences between the participants performance after baseline and before active and sham sessions (Table 2 ). All participants completed three trials of the motion test, except for one participant who performed two trials during all visits and another participant who did only two trials during the baseline visit. Statistical results are presented in Table 1 and Table 2 , and the distribution of the completion rate, completion time and accuracy in Figs. 1 , 2 , and 3 respectively.

Displays the distribution of completion rate. Top: distribution of completion rate for combined movements of the ‘non-dominant side’. Middle and bottom plot: distributions of the completion rate for the ‘dominant sides’ and ‘combined dominant and non-dominant sides’, respectively. Columns 1 and 2 correspond to the baseline session without stimulation, before and after the break, respectively. Columns 3 and 4 represent the active tDCS session, column 3 depicting the completion rate before active stimulation and column 4 after. Columns 5 and 6 represent the sham tDCS session. An asterisk indicates a statistically significant difference between the two neighboring columns.

Displays the distribution of completion time. Top: distribution of completion time for combined movements of the ‘non-dominant side’. Middle and bottom plot: distributions of the completion time for the ‘dominant sides’ and ‘combined dominant and non-dominant sides’, respectively. Columns 1 and 2 correspond to the baseline session without stimulation, before and after the break, respectively. Columns 3 and 4 represent the active tDCS session, column 3 depicting the completion time before active stimulation and column 4 after. Columns 5 and 6 represent the sham tDCS session. An asterisk indicates a statistically significant difference between the two neighboring columns.

Displays the distribution of accuracy. Top: distribution of accuracy for combined movements of the ‘non-dominant side’. Middle and bottom plot: distributions of the accuracy for the ‘dominant sides’ and ‘combined dominant and non-dominant sides’, respectively. Columns 1 and 2 correspond to the baseline session without stimulation, before and after the break, respectively. Columns 3 and 4 represent the active tDCS session, column 3 depicting the accuracy before active stimulation and column 4 after. Columns 5 and 6 represent the sham tDCS session. An asterisk indicates a statistically significant difference between the two neighboring columns.

In the baseline session, statistically significant improvements were observed in the completion rate (28% change, p-value: 0.0029), completion time (14% change, p-value: 0.0125), and accuracy (66% change, p-value: 0.0036). However, during the sham stimulation session, no significant change was detected in any of the MPR parameters. In contrast, the active stimulation session showed a significant improvement of 33% in the completion rate (p-value: 0.0126), but no significant change was detected in either completion time or accuracy.

Subgroup Analysis

Non-dominant side.

In the baseline session, a statistically significant improvement of 37% was observed in the completion rate (p-value: 0.0160). While, during the sham stimulation session, no statistically significant improvement was detected in any of the MPR parameters. In contrast, the active stimulation session showed a statistically significant improvement of 27% in the completion rate (p-value: 0.0236).

Dominant side

In the baseline session, statistically significant improvements were observed in the completion time (16% change, p-value: 0.0205), and accuracy (35% change, p-value: 0.0171), and substantial but not statistically significant improvement in completion rate (20% change, p-value: 0.0708). However, during the sham and active stimulation sessions, no statistically significant improvement was detected in any of the MPR parameters.

This study investigated the effect on the performance of MPR of a single session of anodal tDCS on the primary motor cortex. Testing on the non-dominant primary motor cortex assumes that disparities in the use of the dominant and non-dominant hands can imitate the differences observed between affected and intact hands in individuals with amputations, and the paretic and non-paretic hands in stroke patients. The relatively reduced dexterity of the non-dominant hand, as extensively demonstrated in the literature, stems from its asymmetric usage compared to the dominant hand 35 . Our findings showed a statistically significant improvement in the completion rate of the non-dominant side during the active tDCS day, but not during the sham tDCS day (27%, p = 0.02).

Our results demonstrated a statistically significant improvement in completion rate during the baseline training, but no further improvement during the sham session, which indicates that most learning could have taken place during the baseline training. Since there was no significant improvement in completion rate in the sham tDCS session, we can assume improvements during the active tDCS session are the result of motor excitability 26 , 36 of the non-dominant motor cortex by anodal tDCS. Our findings are in line with previous work on MPR for prosthetics 30 and motor performance in non-disable subjects 31 and stroke patients 37 .

It is worth noting that the largest improvements were observed in the baseline session in which the participants were exposed to the MPR task for the first time (better performance in all metrics). This is not surprising and illustrates that the learning effect of practicing a task for the first time is higher than what is possible to achieve in a later single session of training with neuromodulation. Chronic neuromodulation will likely improve the MPR task further, and potentially show a change in all metrics as opposed to completion rate only. Completion rate relates to the ability of the participant to accomplish a task, whereas completion time and accuracy relate to how effectively it is achieved. Larger improvement in motor control will impact all three metrics, and whether this can be achieved faster with chronic neuromodulation has yet to be investigated.

On the dominant hand all three MPR metrics generated a non-negligible improvement during the baseline training, suggesting that skilled learning ceiling may have been achieved 26 . No further improvement was observed in either sham or active tDCS sessions. In a study by, Pan et al., decreased performance was observed in the unaffected arm in participants with unilateral amputation 30 .

Our findings support the previous literature and suggest that tDCS can enhance MPR performance, particularly in the non-dominant side. The improvements in the MPR we observed indicate the potential of tDCS as an adjunctive tool to enhance motor learning and performance in MPR. Our findings on able bodied participants are supported by the literature in other populations. For example Cho et al . , compared the effect of anodal tDCS over primary motor cortex combined with active or sham Mirror Therapy, and concluded that tDCS plus Mirror Therapy has a positive effect on the functional recovery of upper extremity in stroke patients 38 . Other works have compared the effects of active tDCS with sham tDCS on functional motor and somatosensory functions in acute stroke patients and demonstrated significant improvements using active tDCS (measured by Wolf motor function test and the Semmes Weinstein monofilament test) 39 .

The clinical significance of improving the learning phase in individuals using MPR controlled prosthetic limbs is faster rehabilitation time and potentially better functional outcomes with a reduced prosthetic abandonment rate. In addition, with respect to clinicians, the significance of using tDCS could be reduced consultation time. Moreover, the effectiveness and efficiency of MPR-based PLP treatments, such as Phantom motor execution, can be enhanced 29 , 40 , 41 .

We conducted our study on able-bodied participants, the next step is to translate this method to relevant populations such as stroke patients and individuals with limb loss, in prospective investigations with larger sample sizes and patient matched populations. This was an acute application of tDCS and the chronic effects should be further investigated.

Restoration of function and rehabilitation of pain are of considerable importance after traumatic injuries and stroke. Decoding of motor volition via MPR is a promising tool that is now being used in different assistive and rehabilitation devices, and here we have provided further evidence that tDCS can facilitate MPR and thus potentially improve the clinical outcomes of patients using MPR. Further prospective investigations with larger sample sizes and matched patient populations ( e.g., limb loss or stroke) are necessary to produce higher quality evidence supporting this approach.

The study was approved by the governing ethical committee in Sweden (approval number 2022-00883-02) and was performed in accordance with declaration of Helsinki and the relevant guidelines and regulations. The study was conducted using a double-blinded, randomized and sham-controlled design, wherein participants were unaware of the placebo control. Twelve healthy, able-bodied participants without prior tDCS experience were recruited in this study. When laterality was unclear, the Waterloo handedness questionnaire 42 was used to assess the dominant side of the participant. Those with a history of neurological disorders or contradictions to tDCS were excluded from the study 34 . All participants received detailed information and provided written informed consent, including the consent to publish Fig. 6 .

Study design

The study consisted of three sessions, each conducted on separate days, with 48 h intervals in between sessions to wash out potential carryover effects of tDCS. Participant were randomized to either dominant or non-dominant hand, and for sham or active tDCS, leading to four different groups. The study design is illustrated in Fig. 4 .

Presents an overview of the study design. Each session comprises two rounds of training involving both hands, with a break in between for session one. Sessions two and three receive either sham or active anodal tDCS between the two round of training.

On the first day (baseline session), the participant was familiarized with the experimental setup and procedures. The first session consisted of two rounds of motor training with MPR on both sides, with a break of 20 min in between. On day two and three, participants were exposed to either sham or active tDCS in between the two rounds of motor training. All training sessions for a participant began with the same hand.

Experimental setup

A total of eight bipolar self-adhesive electrodes, along with two reference Ag/AgCl electrodes, were positioned on both arms to record the EMG signals. The electrode diameter was 1 cm with an inter-electrode distance of approximately 2 cm. There were four bipolar electrodes per arm placed on the extensor carpi ulnaris, flexor carpi ulnaris, extensor carpi radialis, flexor carpi radialis muscles, and one reference electrode on the elbow. The positioning of the bipolar electrodes can be seen in Fig. 5 and was based on the orientation of the aforementioned muscles in wrist flexion/extension, elbow flexion/extension, wrist pro/supination and hand open/close movements, as described by previous work using BioPatRec, an open source platform for MPR 15 .

Surface electrode placement on one arm.

Working with one arm at a time, the electrodes were connected to an amplifier (ADS_BP4 43 ) with embedded active filtering (high pass filter at 20 Hz and a low pass filter at 500 Hz) across four channels. The signals were amplified with a gain of 12 and sampled at 1000 Hz.

The setup of the motor training and the motion test can be seen in Fig. 6 A. To stabilize the lower arm, it was positioned in a lower arm rest, supporting the elbow and wrist but enabling enough range to complete the movements. The quality of the EMG signals was assessed by conducting a short myoelectric recording of flexion, extension, open/close movements, and rest. All training was conducted within the BioPatRec environment 17 .

Setup of motor training ( A ), tDCS ( B ) and consumables and electrodes( C ).

The motor training protocol involved movement recording and testing of 10 movements in the following order: hand open, hand close, hand flex, hand extend, supination, pronation, fine grip, side grip, thumb up, pointing with index finger, and state of no movement/relaxation. Each movement was recorded with one dummy repetition and three repetitions lasting for 3 s, with a three-second rest interval in between. After the 10 movements were recorded, four key signal features were extracted (mean absolute value, wavelength, zero crossings, and slope changes) to create the features vectors to train the classifier/decoder (linear discriminant analysis—LDA). LDA has been shown to be a successful decoder for this particular tasks, as outlined in the BioPatRec article 17 . The movement recording was immediately followed by a motion test, where participants were requested to perform all the trained movements in a randomized order: three trials of three repetitions per movement with 5 s time out. The motion test captured the executed movements to examine the performance of MPR in terms of completion rate, completion time, and real-time accuracy as described in the BioPatRec article 17 . After completing the movement recording and motion test on one hand, the process was replicated on the other hand, constituting a singular training round.

Following the completion of the first training round, a 20 min rest on day one was allocated before conducting the second round of the day (starting with the same hand). In days two and three, the break included 20 min of shame or active tDCS. The anodal tDCS (Fig. 6 B) was applied on the non-dominant side, with an anode placed on the contralateral primary motor cortex (C4/C3) and the cathode over the ipsilateral prefrontal cortex (FP1/FP2), using a commercially available system (Starstim ® tES-EEG system). Through saline soaked 25 cm 2 round sponge electrodes, a current of 1 mA was applied for a duration of 20 min, in between a 30 s ramp up- and down for the active tDCS. The ramp up and down happened both in the beginning and end in the sham tDCS, blinding the participant for the actual stimulation status. The baseline training session ended with marking the placement of the electrodes with a skin-friendly marker, to ensure that the electrodes were placed at exactly the same position on day two and three.

Data availability

The data that support the findings of this study are available upon reasonable request.

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Acknowledgements

This study was supported by the Promobilia Foundation, IngaBritt och Arne Lundbergs Forskningsstiftelse, and Vetenskapsrådet.

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M.O.C. conceived the study. S.D. and M.O.C. designed the study. S.D. and K.M. conducted the experiments and analyzed the data. S.D. conducted the literature review and drafted the manuscript. K.A edited the manuscript and provided constructive feedback for it. M.O.C. edited the manuscript, supervised the research, and obtained the funding. All authors reviewed and proved the final version of the manuscript.

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Damercheli, S., Morrenhof, K., Ahmed, K. et al. Performance in myoelectric pattern recognition improves with transcranial direct current stimulation. Sci Rep 14 , 11744 (2024). https://doi.org/10.1038/s41598-024-62185-x

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Mathematics > General Mathematics

Title: on edwards' speculation and a new variational method for the zeros of the $z$-function.

Abstract: In his foundational book, Edwards introduced a unique "speculation" regarding the possible theoretical origins of the Riemann Hypothesis, based on the properties of the Riemann-Siegel formula. Essentially Edwards asks whether one can find a method to transition from zeros of $Z_0(t)=cos(\theta(t))$, where $\theta(t)$ is Riemann-Siegel theta function, to zeros of $Z(t)$, the Hardy $Z$-function. However, when applied directly to the classical Riemann-Siegel formula, it faces significant obstacles in forming a robust plausibility argument for the Riemann Hypothesis. In a recent work, we introduced an alternative to the Riemann-Siegel formula that utilizes series acceleration techniques. In this paper, we explore Edwards' speculation through the lens of our accelerated approach, which avoids many of the challenges encountered in the classical case. Our approach leads to the description of a novel variational framework for relating zeros of $Z_0(t)$ to zeros of $Z(t)$ through paths in a high-dimensional parameter space $\mathcal{Z}_N$, recasting the RH as a modern non-linear optimization problem.

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State true or false: Hypothesis is the initial point of any investigation that translates the research questions into a prediction.

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