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Biology library

Course: biology library   >   unit 1, the scientific method.

  • Controlled experiments
  • The scientific method and experimental design

Introduction

  • Make an observation.
  • Ask a question.
  • Form a hypothesis , or testable explanation.
  • Make a prediction based on the hypothesis.
  • Test the prediction.
  • Iterate: use the results to make new hypotheses or predictions.

Scientific method example: Failure to toast

1. make an observation..

  • Observation: the toaster won't toast.

2. Ask a question.

  • Question: Why won't my toaster toast?

3. Propose a hypothesis.

  • Hypothesis: Maybe the outlet is broken.

4. Make predictions.

  • Prediction: If I plug the toaster into a different outlet, then it will toast the bread.

5. Test the predictions.

  • Test of prediction: Plug the toaster into a different outlet and try again.
  • If the toaster does toast, then the hypothesis is supported—likely correct.
  • If the toaster doesn't toast, then the hypothesis is not supported—likely wrong.

Logical possibility

Practical possibility, building a body of evidence, 6. iterate..

  • Iteration time!
  • If the hypothesis was supported, we might do additional tests to confirm it, or revise it to be more specific. For instance, we might investigate why the outlet is broken.
  • If the hypothesis was not supported, we would come up with a new hypothesis. For instance, the next hypothesis might be that there's a broken wire in the toaster.

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  • How to Write a Strong Hypothesis | Guide & Examples

How to Write a Strong Hypothesis | Guide & Examples

Published on 6 May 2022 by Shona McCombes .

A hypothesis is a statement that can be tested by scientific research. If you want to test a relationship between two or more variables, you need to write hypotheses before you start your experiment or data collection.

Table of contents

What is a hypothesis, developing a hypothesis (with example), hypothesis examples, frequently asked questions about writing hypotheses.

A hypothesis states your predictions about what your research will find. It is a tentative answer to your research question that has not yet been tested. For some research projects, you might have to write several hypotheses that address different aspects of your research question.

A hypothesis is not just a guess – it should be based on existing theories and knowledge. It also has to be testable, which means you can support or refute it through scientific research methods (such as experiments, observations, and statistical analysis of data).

Variables in hypotheses

Hypotheses propose a relationship between two or more variables . An independent variable is something the researcher changes or controls. A dependent variable is something the researcher observes and measures.

In this example, the independent variable is exposure to the sun – the assumed cause . The dependent variable is the level of happiness – the assumed effect .

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Step 1: ask a question.

Writing a hypothesis begins with a research question that you want to answer. The question should be focused, specific, and researchable within the constraints of your project.

Step 2: Do some preliminary research

Your initial answer to the question should be based on what is already known about the topic. Look for theories and previous studies to help you form educated assumptions about what your research will find.

At this stage, you might construct a conceptual framework to identify which variables you will study and what you think the relationships are between them. Sometimes, you’ll have to operationalise more complex constructs.

Step 3: Formulate your hypothesis

Now you should have some idea of what you expect to find. Write your initial answer to the question in a clear, concise sentence.

Step 4: Refine your hypothesis

You need to make sure your hypothesis is specific and testable. There are various ways of phrasing a hypothesis, but all the terms you use should have clear definitions, and the hypothesis should contain:

  • The relevant variables
  • The specific group being studied
  • The predicted outcome of the experiment or analysis

Step 5: Phrase your hypothesis in three ways

To identify the variables, you can write a simple prediction in if … then form. The first part of the sentence states the independent variable and the second part states the dependent variable.

In academic research, hypotheses are more commonly phrased in terms of correlations or effects, where you directly state the predicted relationship between variables.

If you are comparing two groups, the hypothesis can state what difference you expect to find between them.

Step 6. Write a null hypothesis

If your research involves statistical hypothesis testing , you will also have to write a null hypothesis. The null hypothesis is the default position that there is no association between the variables. The null hypothesis is written as H 0 , while the alternative hypothesis is H 1 or H a .

Hypothesis testing is a formal procedure for investigating our ideas about the world using statistics. It is used by scientists to test specific predictions, called hypotheses , by calculating how likely it is that a pattern or relationship between variables could have arisen by chance.

A hypothesis is not just a guess. It should be based on existing theories and knowledge. It also has to be testable, which means you can support or refute it through scientific research methods (such as experiments, observations, and statistical analysis of data).

A research hypothesis is your proposed answer to your research question. The research hypothesis usually includes an explanation (‘ x affects y because …’).

A statistical hypothesis, on the other hand, is a mathematical statement about a population parameter. Statistical hypotheses always come in pairs: the null and alternative hypotheses. In a well-designed study , the statistical hypotheses correspond logically to the research hypothesis.

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Other students also liked, operationalisation | a guide with examples, pros & cons, what is a conceptual framework | tips & examples, a quick guide to experimental design | 5 steps & examples.

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How to Write a Great Hypothesis

Hypothesis Definition, Format, Examples, and Tips

Kendra Cherry, MS, is a psychosocial rehabilitation specialist, psychology educator, and author of the "Everything Psychology Book."

a science hypothesis

Amy Morin, LCSW, is a psychotherapist and international bestselling author. Her books, including "13 Things Mentally Strong People Don't Do," have been translated into more than 40 languages. Her TEDx talk,  "The Secret of Becoming Mentally Strong," is one of the most viewed talks of all time.

a science hypothesis

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  • The Scientific Method

Hypothesis Format

Falsifiability of a hypothesis.

  • Operationalization

Hypothesis Types

Hypotheses examples.

  • Collecting Data

A hypothesis is a tentative statement about the relationship between two or more variables. It is a specific, testable prediction about what you expect to happen in a study. It is a preliminary answer to your question that helps guide the research process.

Consider a study designed to examine the relationship between sleep deprivation and test performance. The hypothesis might be: "This study is designed to assess the hypothesis that sleep-deprived people will perform worse on a test than individuals who are not sleep-deprived."

At a Glance

A hypothesis is crucial to scientific research because it offers a clear direction for what the researchers are looking to find. This allows them to design experiments to test their predictions and add to our scientific knowledge about the world. This article explores how a hypothesis is used in psychology research, how to write a good hypothesis, and the different types of hypotheses you might use.

The Hypothesis in the Scientific Method

In the scientific method , whether it involves research in psychology, biology, or some other area, a hypothesis represents what the researchers think will happen in an experiment. The scientific method involves the following steps:

  • Forming a question
  • Performing background research
  • Creating a hypothesis
  • Designing an experiment
  • Collecting data
  • Analyzing the results
  • Drawing conclusions
  • Communicating the results

The hypothesis is a prediction, but it involves more than a guess. Most of the time, the hypothesis begins with a question which is then explored through background research. At this point, researchers then begin to develop a testable hypothesis.

Unless you are creating an exploratory study, your hypothesis should always explain what you  expect  to happen.

In a study exploring the effects of a particular drug, the hypothesis might be that researchers expect the drug to have some type of effect on the symptoms of a specific illness. In psychology, the hypothesis might focus on how a certain aspect of the environment might influence a particular behavior.

Remember, a hypothesis does not have to be correct. While the hypothesis predicts what the researchers expect to see, the goal of the research is to determine whether this guess is right or wrong. When conducting an experiment, researchers might explore numerous factors to determine which ones might contribute to the ultimate outcome.

In many cases, researchers may find that the results of an experiment  do not  support the original hypothesis. When writing up these results, the researchers might suggest other options that should be explored in future studies.

In many cases, researchers might draw a hypothesis from a specific theory or build on previous research. For example, prior research has shown that stress can impact the immune system. So a researcher might hypothesize: "People with high-stress levels will be more likely to contract a common cold after being exposed to the virus than people who have low-stress levels."

In other instances, researchers might look at commonly held beliefs or folk wisdom. "Birds of a feather flock together" is one example of folk adage that a psychologist might try to investigate. The researcher might pose a specific hypothesis that "People tend to select romantic partners who are similar to them in interests and educational level."

Elements of a Good Hypothesis

So how do you write a good hypothesis? When trying to come up with a hypothesis for your research or experiments, ask yourself the following questions:

  • Is your hypothesis based on your research on a topic?
  • Can your hypothesis be tested?
  • Does your hypothesis include independent and dependent variables?

Before you come up with a specific hypothesis, spend some time doing background research. Once you have completed a literature review, start thinking about potential questions you still have. Pay attention to the discussion section in the  journal articles you read . Many authors will suggest questions that still need to be explored.

How to Formulate a Good Hypothesis

To form a hypothesis, you should take these steps:

  • Collect as many observations about a topic or problem as you can.
  • Evaluate these observations and look for possible causes of the problem.
  • Create a list of possible explanations that you might want to explore.
  • After you have developed some possible hypotheses, think of ways that you could confirm or disprove each hypothesis through experimentation. This is known as falsifiability.

In the scientific method ,  falsifiability is an important part of any valid hypothesis. In order to test a claim scientifically, it must be possible that the claim could be proven false.

Students sometimes confuse the idea of falsifiability with the idea that it means that something is false, which is not the case. What falsifiability means is that  if  something was false, then it is possible to demonstrate that it is false.

One of the hallmarks of pseudoscience is that it makes claims that cannot be refuted or proven false.

The Importance of Operational Definitions

A variable is a factor or element that can be changed and manipulated in ways that are observable and measurable. However, the researcher must also define how the variable will be manipulated and measured in the study.

Operational definitions are specific definitions for all relevant factors in a study. This process helps make vague or ambiguous concepts detailed and measurable.

For example, a researcher might operationally define the variable " test anxiety " as the results of a self-report measure of anxiety experienced during an exam. A "study habits" variable might be defined by the amount of studying that actually occurs as measured by time.

These precise descriptions are important because many things can be measured in various ways. Clearly defining these variables and how they are measured helps ensure that other researchers can replicate your results.

Replicability

One of the basic principles of any type of scientific research is that the results must be replicable.

Replication means repeating an experiment in the same way to produce the same results. By clearly detailing the specifics of how the variables were measured and manipulated, other researchers can better understand the results and repeat the study if needed.

Some variables are more difficult than others to define. For example, how would you operationally define a variable such as aggression ? For obvious ethical reasons, researchers cannot create a situation in which a person behaves aggressively toward others.

To measure this variable, the researcher must devise a measurement that assesses aggressive behavior without harming others. The researcher might utilize a simulated task to measure aggressiveness in this situation.

Hypothesis Checklist

  • Does your hypothesis focus on something that you can actually test?
  • Does your hypothesis include both an independent and dependent variable?
  • Can you manipulate the variables?
  • Can your hypothesis be tested without violating ethical standards?

The hypothesis you use will depend on what you are investigating and hoping to find. Some of the main types of hypotheses that you might use include:

  • Simple hypothesis : This type of hypothesis suggests there is a relationship between one independent variable and one dependent variable.
  • Complex hypothesis : This type suggests a relationship between three or more variables, such as two independent and dependent variables.
  • Null hypothesis : This hypothesis suggests no relationship exists between two or more variables.
  • Alternative hypothesis : This hypothesis states the opposite of the null hypothesis.
  • Statistical hypothesis : This hypothesis uses statistical analysis to evaluate a representative population sample and then generalizes the findings to the larger group.
  • Logical hypothesis : This hypothesis assumes a relationship between variables without collecting data or evidence.

A hypothesis often follows a basic format of "If {this happens} then {this will happen}." One way to structure your hypothesis is to describe what will happen to the  dependent variable  if you change the  independent variable .

The basic format might be: "If {these changes are made to a certain independent variable}, then we will observe {a change in a specific dependent variable}."

A few examples of simple hypotheses:

  • "Students who eat breakfast will perform better on a math exam than students who do not eat breakfast."
  • "Students who experience test anxiety before an English exam will get lower scores than students who do not experience test anxiety."​
  • "Motorists who talk on the phone while driving will be more likely to make errors on a driving course than those who do not talk on the phone."
  • "Children who receive a new reading intervention will have higher reading scores than students who do not receive the intervention."

Examples of a complex hypothesis include:

  • "People with high-sugar diets and sedentary activity levels are more likely to develop depression."
  • "Younger people who are regularly exposed to green, outdoor areas have better subjective well-being than older adults who have limited exposure to green spaces."

Examples of a null hypothesis include:

  • "There is no difference in anxiety levels between people who take St. John's wort supplements and those who do not."
  • "There is no difference in scores on a memory recall task between children and adults."
  • "There is no difference in aggression levels between children who play first-person shooter games and those who do not."

Examples of an alternative hypothesis:

  • "People who take St. John's wort supplements will have less anxiety than those who do not."
  • "Adults will perform better on a memory task than children."
  • "Children who play first-person shooter games will show higher levels of aggression than children who do not." 

Collecting Data on Your Hypothesis

Once a researcher has formed a testable hypothesis, the next step is to select a research design and start collecting data. The research method depends largely on exactly what they are studying. There are two basic types of research methods: descriptive research and experimental research.

Descriptive Research Methods

Descriptive research such as  case studies ,  naturalistic observations , and surveys are often used when  conducting an experiment is difficult or impossible. These methods are best used to describe different aspects of a behavior or psychological phenomenon.

Once a researcher has collected data using descriptive methods, a  correlational study  can examine how the variables are related. This research method might be used to investigate a hypothesis that is difficult to test experimentally.

Experimental Research Methods

Experimental methods  are used to demonstrate causal relationships between variables. In an experiment, the researcher systematically manipulates a variable of interest (known as the independent variable) and measures the effect on another variable (known as the dependent variable).

Unlike correlational studies, which can only be used to determine if there is a relationship between two variables, experimental methods can be used to determine the actual nature of the relationship—whether changes in one variable actually  cause  another to change.

The hypothesis is a critical part of any scientific exploration. It represents what researchers expect to find in a study or experiment. In situations where the hypothesis is unsupported by the research, the research still has value. Such research helps us better understand how different aspects of the natural world relate to one another. It also helps us develop new hypotheses that can then be tested in the future.

Thompson WH, Skau S. On the scope of scientific hypotheses .  R Soc Open Sci . 2023;10(8):230607. doi:10.1098/rsos.230607

Taran S, Adhikari NKJ, Fan E. Falsifiability in medicine: what clinicians can learn from Karl Popper [published correction appears in Intensive Care Med. 2021 Jun 17;:].  Intensive Care Med . 2021;47(9):1054-1056. doi:10.1007/s00134-021-06432-z

Eyler AA. Research Methods for Public Health . 1st ed. Springer Publishing Company; 2020. doi:10.1891/9780826182067.0004

Nosek BA, Errington TM. What is replication ?  PLoS Biol . 2020;18(3):e3000691. doi:10.1371/journal.pbio.3000691

Aggarwal R, Ranganathan P. Study designs: Part 2 - Descriptive studies .  Perspect Clin Res . 2019;10(1):34-36. doi:10.4103/picr.PICR_154_18

Nevid J. Psychology: Concepts and Applications. Wadworth, 2013.

By Kendra Cherry, MSEd Kendra Cherry, MS, is a psychosocial rehabilitation specialist, psychology educator, and author of the "Everything Psychology Book."

Science and the scientific method: Definitions and examples

Here's a look at the foundation of doing science — the scientific method.

Kids follow the scientific method to carry out an experiment.

The scientific method

Hypothesis, theory and law, a brief history of science, additional resources, bibliography.

Science is a systematic and logical approach to discovering how things in the universe work. It is also the body of knowledge accumulated through the discoveries about all the things in the universe. 

The word "science" is derived from the Latin word "scientia," which means knowledge based on demonstrable and reproducible data, according to the Merriam-Webster dictionary . True to this definition, science aims for measurable results through testing and analysis, a process known as the scientific method. Science is based on fact, not opinion or preferences. The process of science is designed to challenge ideas through research. One important aspect of the scientific process is that it focuses only on the natural world, according to the University of California, Berkeley . Anything that is considered supernatural, or beyond physical reality, does not fit into the definition of science.

When conducting research, scientists use the scientific method to collect measurable, empirical evidence in an experiment related to a hypothesis (often in the form of an if/then statement) that is designed to support or contradict a scientific theory .

"As a field biologist, my favorite part of the scientific method is being in the field collecting the data," Jaime Tanner, a professor of biology at Marlboro College, told Live Science. "But what really makes that fun is knowing that you are trying to answer an interesting question. So the first step in identifying questions and generating possible answers (hypotheses) is also very important and is a creative process. Then once you collect the data you analyze it to see if your hypothesis is supported or not."

Here's an illustration showing the steps in the scientific method.

The steps of the scientific method go something like this, according to Highline College :

  • Make an observation or observations.
  • Form a hypothesis — a tentative description of what's been observed, and make predictions based on that hypothesis.
  • Test the hypothesis and predictions in an experiment that can be reproduced.
  • Analyze the data and draw conclusions; accept or reject the hypothesis or modify the hypothesis if necessary.
  • Reproduce the experiment until there are no discrepancies between observations and theory. "Replication of methods and results is my favorite step in the scientific method," Moshe Pritsker, a former post-doctoral researcher at Harvard Medical School and CEO of JoVE, told Live Science. "The reproducibility of published experiments is the foundation of science. No reproducibility — no science."

Some key underpinnings to the scientific method:

  • The hypothesis must be testable and falsifiable, according to North Carolina State University . Falsifiable means that there must be a possible negative answer to the hypothesis.
  • Research must involve deductive reasoning and inductive reasoning . Deductive reasoning is the process of using true premises to reach a logical true conclusion while inductive reasoning uses observations to infer an explanation for those observations.
  • An experiment should include a dependent variable (which does not change) and an independent variable (which does change), according to the University of California, Santa Barbara .
  • An experiment should include an experimental group and a control group. The control group is what the experimental group is compared against, according to Britannica .

The process of generating and testing a hypothesis forms the backbone of the scientific method. When an idea has been confirmed over many experiments, it can be called a scientific theory. While a theory provides an explanation for a phenomenon, a scientific law provides a description of a phenomenon, according to The University of Waikato . One example would be the law of conservation of energy, which is the first law of thermodynamics that says that energy can neither be created nor destroyed. 

A law describes an observed phenomenon, but it doesn't explain why the phenomenon exists or what causes it. "In science, laws are a starting place," said Peter Coppinger, an associate professor of biology and biomedical engineering at the Rose-Hulman Institute of Technology. "From there, scientists can then ask the questions, 'Why and how?'"

Laws are generally considered to be without exception, though some laws have been modified over time after further testing found discrepancies. For instance, Newton's laws of motion describe everything we've observed in the macroscopic world, but they break down at the subatomic level.

This does not mean theories are not meaningful. For a hypothesis to become a theory, scientists must conduct rigorous testing, typically across multiple disciplines by separate groups of scientists. Saying something is "just a theory" confuses the scientific definition of "theory" with the layperson's definition. To most people a theory is a hunch. In science, a theory is the framework for observations and facts, Tanner told Live Science.

This Copernican heliocentric solar system, from 1708, shows the orbit of the moon around the Earth, and the orbits of the Earth and planets round the sun, including Jupiter and its moons, all surrounded by the 12 signs of the zodiac.

The earliest evidence of science can be found as far back as records exist. Early tablets contain numerals and information about the solar system , which were derived by using careful observation, prediction and testing of those predictions. Science became decidedly more "scientific" over time, however.

1200s: Robert Grosseteste developed the framework for the proper methods of modern scientific experimentation, according to the Stanford Encyclopedia of Philosophy. His works included the principle that an inquiry must be based on measurable evidence that is confirmed through testing.

1400s: Leonardo da Vinci began his notebooks in pursuit of evidence that the human body is microcosmic. The artist, scientist and mathematician also gathered information about optics and hydrodynamics.

1500s: Nicolaus Copernicus advanced the understanding of the solar system with his discovery of heliocentrism. This is a model in which Earth and the other planets revolve around the sun, which is the center of the solar system.

1600s: Johannes Kepler built upon those observations with his laws of planetary motion. Galileo Galilei improved on a new invention, the telescope, and used it to study the sun and planets. The 1600s also saw advancements in the study of physics as Isaac Newton developed his laws of motion.

1700s: Benjamin Franklin discovered that lightning is electrical. He also contributed to the study of oceanography and meteorology. The understanding of chemistry also evolved during this century as Antoine Lavoisier, dubbed the father of modern chemistry , developed the law of conservation of mass.

1800s: Milestones included Alessandro Volta's discoveries regarding electrochemical series, which led to the invention of the battery. John Dalton also introduced atomic theory, which stated that all matter is composed of atoms that combine to form molecules. The basis of modern study of genetics advanced as Gregor Mendel unveiled his laws of inheritance. Later in the century, Wilhelm Conrad Röntgen discovered X-rays , while George Ohm's law provided the basis for understanding how to harness electrical charges.

1900s: The discoveries of Albert Einstein , who is best known for his theory of relativity, dominated the beginning of the 20th century. Einstein's theory of relativity is actually two separate theories. His special theory of relativity, which he outlined in a 1905 paper, " The Electrodynamics of Moving Bodies ," concluded that time must change according to the speed of a moving object relative to the frame of reference of an observer. His second theory of general relativity, which he published as " The Foundation of the General Theory of Relativity ," advanced the idea that matter causes space to curve.

In 1952, Jonas Salk developed the polio vaccine , which reduced the incidence of polio in the United States by nearly 90%, according to Britannica . The following year, James D. Watson and Francis Crick discovered the structure of DNA , which is a double helix formed by base pairs attached to a sugar-phosphate backbone, according to the National Human Genome Research Institute .

2000s: The 21st century saw the first draft of the human genome completed, leading to a greater understanding of DNA. This advanced the study of genetics, its role in human biology and its use as a predictor of diseases and other disorders, according to the National Human Genome Research Institute .

  • This video from City University of New York delves into the basics of what defines science.
  • Learn about what makes science science in this book excerpt from Washington State University .
  • This resource from the University of Michigan — Flint explains how to design your own scientific study.

Merriam-Webster Dictionary, Scientia. 2022. https://www.merriam-webster.com/dictionary/scientia

University of California, Berkeley, "Understanding Science: An Overview." 2022. ​​ https://undsci.berkeley.edu/article/0_0_0/intro_01  

Highline College, "Scientific method." July 12, 2015. https://people.highline.edu/iglozman/classes/astronotes/scimeth.htm  

North Carolina State University, "Science Scripts." https://projects.ncsu.edu/project/bio183de/Black/science/science_scripts.html  

University of California, Santa Barbara. "What is an Independent variable?" October 31,2017. http://scienceline.ucsb.edu/getkey.php?key=6045  

Encyclopedia Britannica, "Control group." May 14, 2020. https://www.britannica.com/science/control-group  

The University of Waikato, "Scientific Hypothesis, Theories and Laws." https://sci.waikato.ac.nz/evolution/Theories.shtml  

Stanford Encyclopedia of Philosophy, Robert Grosseteste. May 3, 2019. https://plato.stanford.edu/entries/grosseteste/  

Encyclopedia Britannica, "Jonas Salk." October 21, 2021. https://www.britannica.com/ biography /Jonas-Salk

National Human Genome Research Institute, "​Phosphate Backbone." https://www.genome.gov/genetics-glossary/Phosphate-Backbone  

National Human Genome Research Institute, "What is the Human Genome Project?" https://www.genome.gov/human-genome-project/What  

‌ Live Science contributor Ashley Hamer updated this article on Jan. 16, 2022.

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Three Famous Hypotheses and How They Were Tested

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Art Hasler

Key Takeaways

  • Ivan Pavlov's experiment demonstrated conditioned responses in dogs.
  • Pavlov's work exemplifies the scientific method, starting with a hypothesis about conditioned responses and testing it through controlled experiments.
  • Pavlov's findings not only advanced an understanding of animal physiology but also laid foundational principles for behaviorism, a major school of thought in psychology that emphasizes the study of observable behaviors.

Coho salmon ( Oncorhynchus kisutch ) are amazing fish. Indigenous to the Pacific Northwest, they begin their lives in freshwater streams and then relocate to the open ocean. But when a Coho salmon reaches breeding age, it'll return to the waterway of its birth , sometimes traveling 400 miles (644 kilometers) to get there.

Enter the late Arthur Davis Hasler. While an ecologist and biologist at the University of Wisconsin, he was intrigued by the question of how these creatures find their home streams. And in 1960, he used a Hypothesis-Presentation.pdf">basic tenet of science — the hypothesis — to find out.

So what is a hypothesis? A hypothesis is a tentative, testable explanation for an observed phenomenon in nature. Hypotheses are narrow in scope — unlike theories , which cover a broad range of observable phenomena and draw from many different lines of evidence. Meanwhile, a prediction is a result you'd expect to get if your hypothesis or theory is accurate.

So back to 1960 and Hasler and those salmon. One unverified idea was that Coho salmon used eyesight to locate their home streams. Hasler set out to test this notion (or hypothesis). First, he rounded up several fish who'd already returned to their native streams. Next, he blindfolded some of the captives — but not all of them — before dumping his salmon into a faraway stretch of water. If the eyesight hypothesis was correct, then Hasler could expect fewer of the blindfolded fish to return to their home streams.

Things didn't work out that way. The fish without blindfolds came back at the same rate as their blindfolded counterparts. (Other experiments demonstrated that smell, and not sight, is the key to the species' homing ability.)

Although Hasler's blindfold hypothesis was disproven, others have fared better. Today, we're looking at three of the best-known experiments in history — and the hypotheses they tested.

Ivan Pavlov and His Dogs (1903-1935)

Isaac newton's radiant prisms (1665), robert paine's revealing starfish (1963-1969).

The Hypothesis : If dogs are susceptible to conditioned responses (drooling), then a dog who is regularly exposed to the same neutral stimulus (metronome/bell) before it receives food will associate this neutral stimulus with the act of eating. Eventually, the dog should begin to drool at a predictable rate when it encounters said stimulus — even before any actual food is offered.

The Experiment : A Nobel Prize-winner and outspoken critic of Soviet communism, Ivan Pavlov is synonymous with man's best friend . In 1903, the Russian-born scientist kicked off a decades-long series of experiments involving dogs and conditioned responses .

Offer a plate of food to a hungry dog and it'll salivate. In this context, the stimulus (the food) will automatically trigger a particular response (the drooling). The latter is an innate, unlearned reaction to the former.

By contrast, the rhythmic sound of a metronome or bell is a neutral stimulus. To a dog, the noise has no inherent meaning and if the animal has never heard it before, the sound won't provoke an instinctive reaction. But the sight of food sure will .

So when Pavlov and his lab assistants played the sound of the metronome/bell before feeding sessions, the researchers conditioned test dogs to mentally link metronomes/bells with mealtime. Due to repeated exposure, the noise alone started to make the dogs' mouths water before they were given food.

According to " Ivan Pavlov: A Russian Life in Science " by biographer Daniel P. Todes, Pavlov's big innovation here was his discovery that he could quantify the reaction of each pooch by measuring the amount of saliva it generated. Every canine predictably drooled at its own consistent rate when he or she encountered a personalized (and artificial) food-related cue.

Pavlov and his assistants used conditioned responses to look at other hypotheses about animal physiology, as well. In one notable experiment, a dog was tested on its ability to tell time . This particular pooch always received food when it heard a metronome click at the rate of 60 strokes per minute. But it never got any food after listening to a slower, 40-strokes-per-minute beat. Lo and behold, Pavlov's animal began to salivate in response to the faster rhythm — but not the slower one . So clearly, it could tell the two rhythmic beats apart.

The Verdict : With the right conditioning — and lots of patience — you can make a hungry dog respond to neutral stimuli by salivating on cue in a way that's both predictable and scientifically quantifiable.

Pavlov's dog

The Hypothesis : If white sunlight is a mixture of all the colors in the visible spectrum — and these travel at varying wavelengths — then each color will refract at a different angle when a beam of sunlight passes through a glass prism.

The Experiments : Color was a scientific mystery before Isaac Newton came along. During the summer of 1665, he started experimenting with glass prisms from the safety of a darkened room in Cambridge, England.

He cut a quarter-inch (0.63-centimeter) circular hole into one of the window shutters, allowing a single beam of sunlight to enter the place. When Newton held up a prism to this ray, an oblong patch of multicolored light was projected onto the opposite wall.

This contained segregated layers of red, orange, yellow, green, blue, indigo and violet light. From top to bottom, this patch measured 13.5 inches (33.65 centimeters) tall, yet it was only 2.6 inches (6.6 centimeters) across.

Newton deduced that these vibrant colors had been hiding within the sunlight itself, but the prism bent (or "refracted") them at different angles, which separated the colors out.

Still, he wasn't 100 percent sure. So Newton replicated the experiment with one small change. This time, he took a second prism and had it intercept the rainbow-like patch of light. Once the refracted colors entered the new prism, they recombined into a circular white sunbeam. In other words, Newton took a ray of white light, broke it apart into a bunch of different colors and then reassembled it. What a neat party trick!

The Verdict : Sunlight really is a blend of all the colors in the rainbow — and yes, these can be individually separated via light refraction.

Isaac Newton

The Hypothesis : If predators limit the populations of the organisms they attack, then we'd expect the prey species to become more common after the eradication of a major predator.

The Experiment : Meet Pisaster ochraceus , also known as the purple sea star (or the purple starfish if you prefer).

Using an extendable stomach , the creature feeds on mussels, limpets, barnacles, snails and other hapless victims. On some seaside rocks (and tidal pools) along the coast of Washington state, this starfish is the apex predator.

The animal made Robert Paine a scientific celebrity. An ecologist by trade, Paine was fascinated by the environmental roles of top predators. In June 1963, he kicked off an ambitious experiment along Washington state's Mukkaw Bay. For years on end, Paine kept a rocky section of this shoreline completely starfish-free.

It was hard work. Paine had to regularly pry wayward sea stars off "his" outcrop — sometimes with a crowbar. Then he'd chuck them into the ocean.

Before the experiment, Paine observed 15 different species of animals and algae inhabiting the area he decided to test. By June 1964 — one year after his starfish purge started — that number had dropped to eight .

Unchecked by purple sea stars, the barnacle population skyrocketed. Subsequently, these were replaced by California mussels , which came to dominate the terrain. By anchoring themselves to rocks in great numbers, the mussels edged out other life-forms. That made the outcrop uninhabitable to most former residents: Even sponges, anemones and algae — organisms that Pisaster ochraceus doesn't eat — were largely evicted.

All those species continued to thrive on another piece of shoreline that Paine left untouched. Later experiments convinced him that Pisaster ochraceus is a " keystone species ," a creature who exerts disproportionate influence over its environment. Eliminate the keystone and the whole system gets disheveled.

The Verdict : Apex predators don't just affect the animals that they hunt. Removing a top predator sets off a chain reaction that can fundamentally transform an entire ecosystem.

purple sea stars

Contrary to popular belief, Pavlov almost never used bells in his dog experiments. Instead, he preferred metronomes, buzzers, harmoniums and electric shocks.

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Title: probablistic restoration with adaptive noise sampling for 3d human pose estimation.

Abstract: The accuracy and robustness of 3D human pose estimation (HPE) are limited by 2D pose detection errors and 2D to 3D ill-posed challenges, which have drawn great attention to Multi-Hypothesis HPE research. Most existing MH-HPE methods are based on generative models, which are computationally expensive and difficult to train. In this study, we propose a Probabilistic Restoration 3D Human Pose Estimation framework (PRPose) that can be integrated with any lightweight single-hypothesis model. Specifically, PRPose employs a weakly supervised approach to fit the hidden probability distribution of the 2D-to-3D lifting process in the Single-Hypothesis HPE model and then reverse-map the distribution to the 2D pose input through an adaptive noise sampling strategy to generate reasonable multi-hypothesis samples effectively. Extensive experiments on 3D HPE benchmarks (Human3.6M and MPI-INF-3DHP) highlight the effectiveness and efficiency of PRPose. Code is available at: this https URL .

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Hypothesis Definition (Science)

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A hypothesis is an explanation that is proposed for a phenomenon. Formulating a hypothesis is a step of the scientific method .

Alternate Spellings: plural: hypotheses

Examples: Upon observing that a lake appears blue under a blue sky, you might propose the hypothesis that the lake is blue because it is reflecting the sky. One alternate hypothesis would be that the lake is blue because water is blue.

Hypothesis Versus Theory

Although in common usage the terms hypothesis and theory are used interchangeably, the two words mean something different from each other in science. Like a hypothesis, a theory is testable and may be used to make predictions. However, a theory has been tested using the scientific method many times. Testing a hypothesis may, over time, lead to the formulation of a theory.

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a science hypothesis

The Fermi Paradox and the Berserker Hypothesis: Exploring Cosmic Silence Through Science Fiction

I n the realm of cosmic conundrums, the Fermi Paradox stands out: why, in a universe replete with billions of stars and planets, have we yet to find any signs of extraterrestrial intelligent life? The “berserker hypothesis,” a spine-chilling explanation rooted in science and popularized by science fiction, suggests a grim answer to this enduring mystery.

The concept’s moniker traces back to Fred Saberhagen’s “Berserker” series of novels, and it paints a picture of the cosmos where intelligent life forms are systematically eradicated by self-replicating probes, known as “berserkers.” These probes, initially intended to explore and report back, turn rogue and annihilate any signs of civilizations they encounter. The hypothesis emerges as a rather dark twist on the concept of von Neumann probes—machines capable of self-replication using local resources, which could theoretically colonize the galaxy rapidly.

Diving into the technicalities, the berserker hypothesis operates as a potential solution to the Hart-Tipler conjecture, which posits the lack of detectable probes as evidence that no intelligent life exists outside our solar system. Instead, this hypothesis flips the script: the absence of such probes doesn’t point to a lack of life but rather to the possibility that these probes have become cosmic predators, leaving a trail of silence in their wake.

Astronomer David Brin’s chilling summation underscores the potential severity of the hypothesis: “It need only happen once for the results of this scenario to become the equilibrium conditions in the Galaxy…because all were killed shortly after discovering radio.” If these berserker probes exist and are as efficient as theorized, then humanity’s attempts at communication with extraterrestrial beings could be akin to lighting a beacon for our own destruction.

Despite its foundation in speculative thought, the theory isn’t without its scientific evaluations. Anders Sandberg and Stuart Armstrong from the Future of Humanity Institute speculated that, given the vastness of the universe and even a slow replication rate, these berserker probes—if they existed—would likely have already found and destroyed us. It’s both a chilling and somewhat reassuring analysis that treads the line between fiction and potential reality.

Within the eclectic array of solutions to the Fermi Paradox, the berserker hypothesis stands out for its seamless blend of science fiction inspiration and scientific discourse. It connects with other notions such as the Great Filter, which suggests that life elsewhere in the universe is being systematically snuffed out before it can reach a space-faring stage, and the Dark Forest hypothesis, which posits that civilizations remain silent to avoid detection by such cosmic hunters.

Relevant articles:

– TIL about the berserker hypothesis, a proposed solution to the Fermi paradox stating the reason why we haven’t found other sentient species yet is because those species have been wiped out by self-replicating “berserker” probes.

– The Berserker Hypothesis: The Darkest Explanation Of The Fermi Paradox

– Beyond “Fermi’s Paradox” VI: What is the Berserker Hypothesis?

In the realm of cosmic conundrums, the Fermi Paradox stands out: why, in a universe replete with billions of stars and planets, have we yet to find any signs of extraterrestrial intelligent life? The “berserker hypothesis,” a spine-chilling explanation rooted in science and popularized by science fiction, suggests a grim answer to this enduring mystery. […]

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  1. Scientific hypothesis

    The Royal Society - On the scope of scientific hypotheses (Apr. 24, 2024) scientific hypothesis, an idea that proposes a tentative explanation about a phenomenon or a narrow set of phenomena observed in the natural world. The two primary features of a scientific hypothesis are falsifiability and testability, which are reflected in an "If ...

  2. What Is a Hypothesis? The Scientific Method

    A hypothesis (plural hypotheses) is a proposed explanation for an observation. The definition depends on the subject. In science, a hypothesis is part of the scientific method. It is a prediction or explanation that is tested by an experiment. Observations and experiments may disprove a scientific hypothesis, but can never entirely prove one.

  3. How to Write a Strong Hypothesis

    Developing a hypothesis (with example) Step 1. Ask a question. Writing a hypothesis begins with a research question that you want to answer. The question should be focused, specific, and researchable within the constraints of your project. Example: Research question.

  4. What is a scientific hypothesis?

    A scientific hypothesis is a tentative, testable explanation for a phenomenon in the natural world. It's the initial building block in the scientific method. Many describe it as an "educated guess ...

  5. The scientific method (article)

    The scientific method. At the core of biology and other sciences lies a problem-solving approach called the scientific method. The scientific method has five basic steps, plus one feedback step: Make an observation. Ask a question. Form a hypothesis, or testable explanation. Make a prediction based on the hypothesis.

  6. How to Write a Strong Hypothesis

    Step 5: Phrase your hypothesis in three ways. To identify the variables, you can write a simple prediction in if … then form. The first part of the sentence states the independent variable and the second part states the dependent variable. If a first-year student starts attending more lectures, then their exam scores will improve.

  7. Hypothesis

    The hypothesis of Andreas Cellarius, showing the planetary motions in eccentric and epicyclical orbits.. A hypothesis (pl.: hypotheses) is a proposed explanation for a phenomenon.For a hypothesis to be a scientific hypothesis, the scientific method requires that one can test it. Scientists generally base scientific hypotheses on previous observations that cannot satisfactorily be explained ...

  8. Steps of the Scientific Method

    The six steps of the scientific method include: 1) asking a question about something you observe, 2) doing background research to learn what is already known about the topic, 3) constructing a hypothesis, 4) experimenting to test the hypothesis, 5) analyzing the data from the experiment and drawing conclusions, and 6) communicating the results ...

  9. Hypothesis: Definition, Examples, and Types

    A hypothesis is a tentative statement about the relationship between two or more variables. It is a specific, testable prediction about what you expect to happen in a study. It is a preliminary answer to your question that helps guide the research process. Consider a study designed to examine the relationship between sleep deprivation and test ...

  10. Scientific Hypothesis, Theory, Law Definitions

    Words have precise meanings in science. For example, "theory," "law," and "hypothesis" don't all mean the same thing. Outside of science, you might say something is "just a theory," meaning it's a supposition that may or may not be true. In science, however, a theory is an explanation that generally is accepted to be true.

  11. Science and the scientific method: Definitions and examples

    Science is a systematic and logical approach to discovering how things in the universe work. Scientists use the scientific method to make observations, form hypotheses and gather evidence in an ...

  12. Writing a Hypothesis for Your Science Fair Project

    A hypothesis is a tentative, testable answer to a scientific question. Once a scientist has a scientific question she is interested in, the scientist reads up to find out what is already known on the topic. Then she uses that information to form a tentative answer to her scientific question. Sometimes people refer to the tentative answer as "an ...

  13. Writing a Hypothesis for Your Science Fair Project

    The goal of a science project is not to prove your hypothesis right or wrong. The goal is to learn more about how the natural world works. Even in a science fair, judges can be impressed by a project that started with a bad hypothesis. What matters is that you understood your project, did a good experiment, and have ideas for how to make it better.

  14. Three Famous Hypotheses and How They Were Tested

    A hypothesis is a tentative, testable explanation for an observed phenomenon in nature. Hypotheses are narrow in scope — unlike theories, which cover a broad range of observable phenomena and draw from many different lines of evidence. Meanwhile, a prediction is a result you'd expect to get if your hypothesis or theory is accurate.

  15. Scientific Hypothesis Examples

    Scientific Hypothesis Examples . Hypothesis: All forks have three tines. This would be disproven if you find any fork with a different number of tines. Hypothesis: There is no relationship between smoking and lung cancer.While it is difficult to establish cause and effect in health issues, you can apply statistics to data to discredit or support this hypothesis.

  16. Writing a hypothesis and prediction

    A hypothesis is an idea about how something works that can be tested using experiments. A prediction says what will happen in an experiment if the hypothesis is correct. Presenter 1: We are going ...

  17. A Strong Hypothesis

    Keep in mind that writing the hypothesis is an early step in the process of doing a science project. The steps below form the basic outline of the Scientific Method: Ask a Question. Do Background Research. Construct a Hypothesis. Test Your Hypothesis by Doing an Experiment. Analyze Your Data and Draw a Conclusion.

  18. Theory vs. Hypothesis: Basics of the Scientific Method

    Theory vs. Hypothesis: Basics of the Scientific Method. Written by MasterClass. Last updated: Jun 7, 2021 • 2 min read. Though you may hear the terms "theory" and "hypothesis" used interchangeably, these two scientific terms have drastically different meanings in the world of science.

  19. What Are Examples of a Hypothesis?

    Here are examples of a scientific hypothesis. Although you could state a scientific hypothesis in various ways, most hypotheses are either "If, then" statements or forms of the null hypothesis. The null hypothesis is sometimes called the "no difference" hypothesis. The null hypothesis is good for experimentation because it's simple to disprove.

  20. Hypothesis Testing

    Table of contents. Step 1: State your null and alternate hypothesis. Step 2: Collect data. Step 3: Perform a statistical test. Step 4: Decide whether to reject or fail to reject your null hypothesis. Step 5: Present your findings. Other interesting articles. Frequently asked questions about hypothesis testing.

  21. When the mind's eye prevails: The Internal Dominance over ...

    /r/EverythingScience is the sister subreddit to /r/science. With a broader rule set than /r/science, it is the place for high quality scientific content that doesn't necessarily reference a peer-reviewed paper from the last 6 months. ... It is hoped that this novel hypothesis motivates cross-talk between the reviewed research lines and future ...

  22. Probablistic Restoration with Adaptive Noise Sampling for 3D Human Pose

    The accuracy and robustness of 3D human pose estimation (HPE) are limited by 2D pose detection errors and 2D to 3D ill-posed challenges, which have drawn great attention to Multi-Hypothesis HPE research. Most existing MH-HPE methods are based on generative models, which are computationally expensive and difficult to train. In this study, we propose a Probabilistic Restoration 3D Human Pose ...

  23. Hypothesis Definition (Science)

    Hypothesis Versus Theory . Although in common usage the terms hypothesis and theory are used interchangeably, the two words mean something different from each other in science. Like a hypothesis, a theory is testable and may be used to make predictions. However, a theory has been tested using the scientific method many times.

  24. The Fermi Paradox and the Berserker Hypothesis: Exploring Cosmic ...

    The "berserker hypothesis," a spine-chilling explanation rooted in science and popularized by science fiction, suggests a grim answer to this enduring mystery. The concept's moniker traces ...