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Medical Management of Tuberculosis: An Online Presentation

This slide presentation with streaming audio, provides information on how to manage treatment of tuberculosis (TB). A pre and posttest, question and answer guide, printable PowerPoint slide file, and other useful resources are also included as supplemental reading materials.

Home / Free PowerPoint Templates / Minimal Tuberculosis Disease Slides

Minimal Tuberculosis Disease Slides

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Introducing our Tuberculosis Disease template, designed specifically for health professionals. With its dominant blue and white color scheme and minimal gradient style, this PowerPoint and Google Slides template is perfect for creating informative presentations on this critical topic. Whether you’re conducting scientific research or delivering educational content, this template provides a clean and professional backdrop to showcase your findings. Engage your audience with visually appealing slides and make a lasting impact. Take your presentations to the next level with our Tuberculosis Disease template. Get started today and make a difference in the fight against this global health issue.

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Tuberculosis in the United States 2020 Slide Set

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NOTE: The slide set is in the public domain. You may reproduce these slides without permission; however it is requested that you include an attribution that the materials were developed by CDC (e.g., “Source: CDC” or “Slides developed by CDC”). No substantive changes should be made to the content.

This slide series was developed as an accompaniment to the document Reported Tuberculosis in the United States, 2020

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DIAGNOSIS OF TUBERCULOSIS

Oct 29, 2019

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DIAGNOSIS OF TUBERCULOSIS. The first step of TB diagnosis is to suspect a case. When to suspect TB (pulmonary TB)?. CLINICAL PRESENTATION OF TB Pulmonary TB. Symptoms. Physical signs. Physical signs are non-specific and may not be helpful in confirming the diagnosis

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Presentation Transcript

The first step of TB diagnosis is to suspect a case When to suspect TB (pulmonary TB)?

CLINICAL PRESENTATION OF TBPulmonary TB Symptoms Physical signs Physical signs are non-specific and may not be helpful in confirming the diagnosis Chest – there may be crackles in the lung apices more pronounced on deep breathing; localized wheeze in local obstruction or pressure; dullness where there is effusion and in chronic disease there may be extensive fibrosis with the trachea pulled to one side. • Persistent cough of 2 weeks or more with or without Production of sputum which may be blood-stained not responding to non-specific treatment (including antibiotics with no anti-TB effect i.e. avoid Rifampicin, aminoglycosides and Quinolones) • Fever for more than 2 weeks mainly at night. • Night sweats • Breathlessness • chest pain • loss of appetite and loss of weight • History of contact sometimes could be detected.

What to do for a presumptive (Suspect) pulmonary tuberculosis case? • The first step is to register the presumptive in the presumptive case register • Request a sputum examination for Acid Fast Bacilli, AFB (Smear stained by Z-N stain).Every pulmonary TB presumptive should submit three sputum samples (at least two samples)

the recommended method for sputum collection is described in the following table. It should be noted that sure TB diagnosis depends on visualization of the Mycobacteria on a smear or culture.

The expected sputum smear examination results could be one of four. The following table shows the probable results and the corresponding action

Causes of false positive direct sputum smear examination for AFB with ZN stain, • A false positive result means that the sputum smear result is positive even though the patient does not really have pulmonary TB. This may arise because of the following: • red stain retained by scratches on the slide, • accidental transfer of AFBs from a positive slide to a negative one (contamination), • Contamination of the slide or smear by environmental mycobacteria, • Various particles that are acid-fast (e.g. food particles, stain precipitates, other micro-organisms).

Smear negative pulmonary tuberculosis • When the patient presents with symptoms consistent with tuberculosis and dealt with as a presumptive case as previously mentioned, then all the received sputum smears results are negative, we come to a problem. To deal with this situation follow the following steps: • Do an x-ray chest If has not been done yet. X-ray chest may show any lesion pattern (infiltrations, pneumonic patch (s), pleural effusion, atelectasis, cavitation…etc.). It should be noted that there is no peculiar x-ray pattern specific for TB. • Do sputum examination by GeneXpert. • If there is neither clinical nor radiological improvement and sputum re-examination is still negative by all means, investigate the case to exclude other underlying causes for the presenting complaints e.g. malignancy, sarcoidosis, other lung infections…etc.

Solid Conventional Culture (Lowenstein-Jansen medium) • Culture of sputum is more sensitive (require from 10-100 mycobacteria /ml sputum) than smear examination (which will be positive if there are 104-105 mycobacteria/1 ml sputum), • It takes from four to eight weeks before the result is known. • It also requires well-equipped laboratories with skilled staff.

Indications of culture • For all cases of extra-pulmonary tuberculosis. • For all cases of smear negative pulmonary tuberculosis (together with GeneXpert or a substitute for it) • TB/HIV co-infection • For differentiatingMycobacteria tuberculosis complex from other non-Tuberculosis Mycobacteria. • Culture is done with drug susceptibility testing, DST, for new smear positive pulmonary tuberculosis who remain positive at the end of the second month of treatment with Cat I treatment course and for all retreatment (after relapse, after failure and after default) before starting Cat II retreatment course.

Culture on liquid medium The MGIT 960 System • The MGIT 960 system is a non-radio-metric(does not use radio-active material) automated system that uses the MGIT media & sensors to detect the fluorescence. • It can differentiate between TB complex & non-TB mycobacteria. • Drug susceptibility tests are available for Streptomycin, INH, Rifampicin, Ethambetuland and Pyrazinamide. • The average time of detection with the MGIT 960 system is 12.7 days compared to 20 days with the solid media.

GeneXpert MTB/RIF • GeneXpertMTB/RIF is an automated molecular platform to detect M. tuberculosis and rifampicin resistance testing by targeting specific mutations in the rpoB gene. • It is approved for use directly on raw sputum and results should be available within 2 hours in the laboratory but available in health facilities within 48 hours. • The test involves only three manual steps: • Addition of reagent to liquefy and inactivate the sputum. • Transfer of 2ml of liquefied sputum to the cartridge • Loading the cartridge into the device for the assay.

Advantages of the test • It detects MTB and Rifampicin resistance from one specimen at the same time. • Processing time for the test itself is 2 hours. • It is specific for MTB complex. • It can also be used on the following processed samples - CSF, aspirates (gastric, lymph node) and tissue (i.e. pleural biopsy) • The test for each specimen is carried out in a closed system (cartridge), so there is a reduced risk of cross-contamination and human error. The limitations of this test are that • It cannot be used for monitoring treatment because it does not distinguish between live and dead bacilli, its use is therefore limited to diagnosis • The assay is semi-quantitative and defines a positive test as “very low”, “low”, “medium”, and “high”. This grading is not reported on the laboratory result. There is no direct correlation between the Xpert semi-quantitative result and the smear grading of scanty, +, ++ and +++. • The test might be unsuccessful due to laboratory test errors, test failure or invalid results. In these instances a second specimen must be collected for a repeat Xpert test.

Line Probe Assay (not yet available in NTP of Egypt) • This test has been approved for direct testing on smear positive specimens and on isolates from solid and liquid culture. It simultaneously detects MTB complex and specific mutations in the rpoB gene conferring rifampicin resistance and mutations on the katG gene which is associated with higher levels of isoniazid resistance and inhA gene mutations which is associated with lower levels of isoniazid resistance. • Compared to phenotypic DST this provides rapid diagnosis of drug resistant TB and results should be available within 48-72 hours in the laboratory. Advantages of the test are that; • It detects MTB and resistance to R & INH at the same time from one specimen • It reduces time to diagnosis of MDR-TB • It is specific for MTB complex. The limitations of the test are that; • The test is labor intensive and is prone to contamination and human error • It requires a lot of space - at least 3 separate rooms for the different steps

Radiology no radiological picture can be characteristic of the disease. • Chest radiograph can be helpful in localizing abnormalities but not to establish the diagnosis of tuberculosis. • Only bacteriology can provide the final proof. Radiological findings are relevant only to a certain extent and are therefore recorded as: • Chest x-rays are necessary in TB who cannot produce sputum or who have negative smears, and where extra-pulmonary TB (such as pleural effusions and pericardial TB) is suspected. They must be interpreted according to the patient’s history and other clinical findings.

Indications for the use of chest x-rays • To assist in the diagnosis of TB: • When smears are negative • Where extra-pulmonary or miliary TB is suspected • For primary TB in children • During or at the end of treatment to evaluate the response to treatment or when response to treatment is not satisfactory. • To assist in the diagnosis of suspected complications: • In a breathless patient to exclude a pneumothorax or pleural effusion. • For frequent or severe hemoptysis. • Computed tomography (CT) scanfindings in tuberculosis are equally non-specific. However, in cases of mediastinal Lymphadenopathy, peripheral rim enhancement with relatively low attenuation centers can suggest a diagnosis of tuberculosis in the appropriate clinical setting. Also in extra-pulmonary case e.g. tuberculosis osteoarthritis (particularly MRI).

TUBERCULIN SKIN TESTING • The tuberculin skin test (TST) has limited value in clinical work, especially where TB is common. The test shows hypersensitivity to proteins of the TB bacillus, as a result either of infection with M. tuberculosis or induced by BacilleCalmette-Guérin (BCG) vaccination. • The test involves injecting tuberculin purified protein derivative (PPD) into the skin. Previous exposure results in a local delayed type hypersensitivity reaction within 24-72 hours. • The reaction is identified as palpable induration (hardness) at the site of injection. The response only indicates hypersensitivity. It shows that the person has at some time been infected with M. tuberculosis or been vaccinated.By itself, it does not indicate the presence or extent of tuberculosis disease. • It should also be noted that a negative result does not rule out the diagnosis of TB disease.Performing a Mantoux Tuberculin Skin Test

The Mantoux TST is the most reliable test available. The test requires: • 2 units of tuberculin purified protein derivative PPD-RT23 2TU or • 5 units of PPD-S 5TU. • Use a single-dose tuberculin syringe and a short 27-gauge needle with a short bevel to do the test. • Draw up 0.1 ml of PPD of the correct strength into the syringe. • Clean an area of skin in the mid anterior section of the forearm. The PPD is injected between layers of skin (intradermal). Keep the needle almost parallel to the skin, with the bevel pointing upwards during insertion. It is important to ensure that the injection goes into and not under the skin. A small papule should form at the injection site; if it does not, the PPD has been injected too deeply and the test should repeated at a different site. • The reaction to the test at the site of the injection is measured 48-72 hours later by noting the widest transverse point across the edges of the raised, thickened area. This area of induration and not redness is measured. • To help measure accurately, mark the edges of the induration at the widest point with a pen and measure the exact distance between the two points in millimeters.

Limitation of TST • TST will be positive in cases with either infection or active disease, so it cannot differentiate between both conditions. • TST also cannot differentiate infection with M tuberculosis from other mycobacteria. • After BCG vaccination, it is not possible to distinguish between a tuberculin skin test reaction caused by virulent mycobacterial infection or by vaccination itself. False negative Reactions to Tuberculin: 1- Factors related to the person being tested: • Viral (HIV, measles, chicken pox). • Bacterial (typhoid fever, brucellosis, typhus, leprosy pertussis, overwhelming TB). • Live virus vaccinations (measles, mumps, polio). • Metabolic derangements (chronic renal failure). • Nutritional factors (severe protein depletion). • Disease affecting lymphoid organs (Hodgkin’s disease, lymphoma, sarcoidosis) • Recent or overwhelming infection with M. tuberculosis. • Stress (surgery, burns…etc.).

2 - Factors related to the tuberculin used: • Improper storage (exposure to light and heat). • Improper dilutions. • Chemical de-naturation (PPD). • Contamination. 3 - Factors related to the method of administration: • Injection of too little antigen. , • Delayed administration after drawing into syringe. • Injection too deep 4 - Factors related to reading the test and recording results: • Inexperienced reading. • Conscious or unconscious bias. • Error in reading

Other tests those are available Interferon gamma Release Assays (IGRA) • Interferon-Gamma Release Assay (IGRAs) is whole-blood test that can aid in diagnosing Mycobacterium tuberculosis infection. • They do not help differentiate latent tuberculosis infection (LTBI) from tuberculosis disease. • It is not affected by previous BCG vaccination. • Test is expected to be more specific than a TST because the antigen used in this test is RELATIVELY SPECIFIC to M. tuberculosis and should produce fewer false-positive tests (i.e., they should not produce cross-reactions after sensitization by BCG and most non-tuberculosis mycobacteria, such as M. avium complex). However, in a study in which cohorts with similar risks for infection were compared, the specificity of IGRA using ESAT-6 or CFP-10 did not differ significantly between those vaccinated with BCG and those not vaccinated. The effect of BCG on specificity is difficult to assess because BCG is used predominately in populations already at increased risk for M. tuberculosis infection. • WHO does not recommend these tests for program purposes in low and middle income settings.

Disadvantages and limitations of IGRAs • Fresh blood samples must be processed within 8-30 hours after collection while white blood cells are still viable. • Errors in collecting or transporting blood specimens or in running and interpreting the assay can decrease the accuracy of IGRAs. • Limited data on the use of IGRAs for: • Children younger than 5 years of age; • Persons recently exposed to M. tuberculosis; • Immuno-compromised persons; • Tests are expensive. • Estimates of IGRA sensitivity have varied widely in published studies which have involved predominantly adults with culture-confirmed active tuberculosis. In general, IGRA sensitivities are considered similar to those for TST.

Blood culture • Blood cultures may be used to detect MTB and other species of mycobacteria in HIV-infected patients; especially those with low CD4 count where disseminated disease is suspected.

Histological examination • Histo-pathological examination may be conducted on tissue specimen. • Samples that can be submitted for examination include; Fine needle aspiration from lymph nodes Tissue biopsies from serous membranes, skin, pleura, endometrium, liver

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