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  • How to Write a Strong Hypothesis | Steps & Examples

How to Write a Strong Hypothesis | Steps & Examples

Published on May 6, 2022 by Shona McCombes . Revised on November 20, 2023.

A hypothesis is a statement that can be tested by scientific research. If you want to test a relationship between two or more variables, you need to write hypotheses before you start your experiment or data collection .

Example: Hypothesis

Daily apple consumption leads to fewer doctor’s visits.

Table of contents

What is a hypothesis, developing a hypothesis (with example), hypothesis examples, other interesting articles, frequently asked questions about writing hypotheses.

A hypothesis states your predictions about what your research will find. It is a tentative answer to your research question that has not yet been tested. For some research projects, you might have to write several hypotheses that address different aspects of your research question.

A hypothesis is not just a guess – it should be based on existing theories and knowledge. It also has to be testable, which means you can support or refute it through scientific research methods (such as experiments, observations and statistical analysis of data).

Variables in hypotheses

Hypotheses propose a relationship between two or more types of variables .

  • An independent variable is something the researcher changes or controls.
  • A dependent variable is something the researcher observes and measures.

If there are any control variables , extraneous variables , or confounding variables , be sure to jot those down as you go to minimize the chances that research bias  will affect your results.

In this example, the independent variable is exposure to the sun – the assumed cause . The dependent variable is the level of happiness – the assumed effect .

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Step 1. ask a question.

Writing a hypothesis begins with a research question that you want to answer. The question should be focused, specific, and researchable within the constraints of your project.

Step 2. Do some preliminary research

Your initial answer to the question should be based on what is already known about the topic. Look for theories and previous studies to help you form educated assumptions about what your research will find.

At this stage, you might construct a conceptual framework to ensure that you’re embarking on a relevant topic . This can also help you identify which variables you will study and what you think the relationships are between them. Sometimes, you’ll have to operationalize more complex constructs.

Step 3. Formulate your hypothesis

Now you should have some idea of what you expect to find. Write your initial answer to the question in a clear, concise sentence.

4. Refine your hypothesis

You need to make sure your hypothesis is specific and testable. There are various ways of phrasing a hypothesis, but all the terms you use should have clear definitions, and the hypothesis should contain:

  • The relevant variables
  • The specific group being studied
  • The predicted outcome of the experiment or analysis

5. Phrase your hypothesis in three ways

To identify the variables, you can write a simple prediction in  if…then form. The first part of the sentence states the independent variable and the second part states the dependent variable.

In academic research, hypotheses are more commonly phrased in terms of correlations or effects, where you directly state the predicted relationship between variables.

If you are comparing two groups, the hypothesis can state what difference you expect to find between them.

6. Write a null hypothesis

If your research involves statistical hypothesis testing , you will also have to write a null hypothesis . The null hypothesis is the default position that there is no association between the variables. The null hypothesis is written as H 0 , while the alternative hypothesis is H 1 or H a .

  • H 0 : The number of lectures attended by first-year students has no effect on their final exam scores.
  • H 1 : The number of lectures attended by first-year students has a positive effect on their final exam scores.

If you want to know more about the research process , methodology , research bias , or statistics , make sure to check out some of our other articles with explanations and examples.

  • Sampling methods
  • Simple random sampling
  • Stratified sampling
  • Cluster sampling
  • Likert scales
  • Reproducibility

 Statistics

  • Null hypothesis
  • Statistical power
  • Probability distribution
  • Effect size
  • Poisson distribution

Research bias

  • Optimism bias
  • Cognitive bias
  • Implicit bias
  • Hawthorne effect
  • Anchoring bias
  • Explicit bias

A hypothesis is not just a guess — it should be based on existing theories and knowledge. It also has to be testable, which means you can support or refute it through scientific research methods (such as experiments, observations and statistical analysis of data).

Null and alternative hypotheses are used in statistical hypothesis testing . The null hypothesis of a test always predicts no effect or no relationship between variables, while the alternative hypothesis states your research prediction of an effect or relationship.

Hypothesis testing is a formal procedure for investigating our ideas about the world using statistics. It is used by scientists to test specific predictions, called hypotheses , by calculating how likely it is that a pattern or relationship between variables could have arisen by chance.

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Home » What is a Hypothesis – Types, Examples and Writing Guide

What is a Hypothesis – Types, Examples and Writing Guide

Table of Contents

What is a Hypothesis

Definition:

Hypothesis is an educated guess or proposed explanation for a phenomenon, based on some initial observations or data. It is a tentative statement that can be tested and potentially proven or disproven through further investigation and experimentation.

Hypothesis is often used in scientific research to guide the design of experiments and the collection and analysis of data. It is an essential element of the scientific method, as it allows researchers to make predictions about the outcome of their experiments and to test those predictions to determine their accuracy.

Types of Hypothesis

Types of Hypothesis are as follows:

Research Hypothesis

A research hypothesis is a statement that predicts a relationship between variables. It is usually formulated as a specific statement that can be tested through research, and it is often used in scientific research to guide the design of experiments.

Null Hypothesis

The null hypothesis is a statement that assumes there is no significant difference or relationship between variables. It is often used as a starting point for testing the research hypothesis, and if the results of the study reject the null hypothesis, it suggests that there is a significant difference or relationship between variables.

Alternative Hypothesis

An alternative hypothesis is a statement that assumes there is a significant difference or relationship between variables. It is often used as an alternative to the null hypothesis and is tested against the null hypothesis to determine which statement is more accurate.

Directional Hypothesis

A directional hypothesis is a statement that predicts the direction of the relationship between variables. For example, a researcher might predict that increasing the amount of exercise will result in a decrease in body weight.

Non-directional Hypothesis

A non-directional hypothesis is a statement that predicts the relationship between variables but does not specify the direction. For example, a researcher might predict that there is a relationship between the amount of exercise and body weight, but they do not specify whether increasing or decreasing exercise will affect body weight.

Statistical Hypothesis

A statistical hypothesis is a statement that assumes a particular statistical model or distribution for the data. It is often used in statistical analysis to test the significance of a particular result.

Composite Hypothesis

A composite hypothesis is a statement that assumes more than one condition or outcome. It can be divided into several sub-hypotheses, each of which represents a different possible outcome.

Empirical Hypothesis

An empirical hypothesis is a statement that is based on observed phenomena or data. It is often used in scientific research to develop theories or models that explain the observed phenomena.

Simple Hypothesis

A simple hypothesis is a statement that assumes only one outcome or condition. It is often used in scientific research to test a single variable or factor.

Complex Hypothesis

A complex hypothesis is a statement that assumes multiple outcomes or conditions. It is often used in scientific research to test the effects of multiple variables or factors on a particular outcome.

Applications of Hypothesis

Hypotheses are used in various fields to guide research and make predictions about the outcomes of experiments or observations. Here are some examples of how hypotheses are applied in different fields:

  • Science : In scientific research, hypotheses are used to test the validity of theories and models that explain natural phenomena. For example, a hypothesis might be formulated to test the effects of a particular variable on a natural system, such as the effects of climate change on an ecosystem.
  • Medicine : In medical research, hypotheses are used to test the effectiveness of treatments and therapies for specific conditions. For example, a hypothesis might be formulated to test the effects of a new drug on a particular disease.
  • Psychology : In psychology, hypotheses are used to test theories and models of human behavior and cognition. For example, a hypothesis might be formulated to test the effects of a particular stimulus on the brain or behavior.
  • Sociology : In sociology, hypotheses are used to test theories and models of social phenomena, such as the effects of social structures or institutions on human behavior. For example, a hypothesis might be formulated to test the effects of income inequality on crime rates.
  • Business : In business research, hypotheses are used to test the validity of theories and models that explain business phenomena, such as consumer behavior or market trends. For example, a hypothesis might be formulated to test the effects of a new marketing campaign on consumer buying behavior.
  • Engineering : In engineering, hypotheses are used to test the effectiveness of new technologies or designs. For example, a hypothesis might be formulated to test the efficiency of a new solar panel design.

How to write a Hypothesis

Here are the steps to follow when writing a hypothesis:

Identify the Research Question

The first step is to identify the research question that you want to answer through your study. This question should be clear, specific, and focused. It should be something that can be investigated empirically and that has some relevance or significance in the field.

Conduct a Literature Review

Before writing your hypothesis, it’s essential to conduct a thorough literature review to understand what is already known about the topic. This will help you to identify the research gap and formulate a hypothesis that builds on existing knowledge.

Determine the Variables

The next step is to identify the variables involved in the research question. A variable is any characteristic or factor that can vary or change. There are two types of variables: independent and dependent. The independent variable is the one that is manipulated or changed by the researcher, while the dependent variable is the one that is measured or observed as a result of the independent variable.

Formulate the Hypothesis

Based on the research question and the variables involved, you can now formulate your hypothesis. A hypothesis should be a clear and concise statement that predicts the relationship between the variables. It should be testable through empirical research and based on existing theory or evidence.

Write the Null Hypothesis

The null hypothesis is the opposite of the alternative hypothesis, which is the hypothesis that you are testing. The null hypothesis states that there is no significant difference or relationship between the variables. It is important to write the null hypothesis because it allows you to compare your results with what would be expected by chance.

Refine the Hypothesis

After formulating the hypothesis, it’s important to refine it and make it more precise. This may involve clarifying the variables, specifying the direction of the relationship, or making the hypothesis more testable.

Examples of Hypothesis

Here are a few examples of hypotheses in different fields:

  • Psychology : “Increased exposure to violent video games leads to increased aggressive behavior in adolescents.”
  • Biology : “Higher levels of carbon dioxide in the atmosphere will lead to increased plant growth.”
  • Sociology : “Individuals who grow up in households with higher socioeconomic status will have higher levels of education and income as adults.”
  • Education : “Implementing a new teaching method will result in higher student achievement scores.”
  • Marketing : “Customers who receive a personalized email will be more likely to make a purchase than those who receive a generic email.”
  • Physics : “An increase in temperature will cause an increase in the volume of a gas, assuming all other variables remain constant.”
  • Medicine : “Consuming a diet high in saturated fats will increase the risk of developing heart disease.”

Purpose of Hypothesis

The purpose of a hypothesis is to provide a testable explanation for an observed phenomenon or a prediction of a future outcome based on existing knowledge or theories. A hypothesis is an essential part of the scientific method and helps to guide the research process by providing a clear focus for investigation. It enables scientists to design experiments or studies to gather evidence and data that can support or refute the proposed explanation or prediction.

The formulation of a hypothesis is based on existing knowledge, observations, and theories, and it should be specific, testable, and falsifiable. A specific hypothesis helps to define the research question, which is important in the research process as it guides the selection of an appropriate research design and methodology. Testability of the hypothesis means that it can be proven or disproven through empirical data collection and analysis. Falsifiability means that the hypothesis should be formulated in such a way that it can be proven wrong if it is incorrect.

In addition to guiding the research process, the testing of hypotheses can lead to new discoveries and advancements in scientific knowledge. When a hypothesis is supported by the data, it can be used to develop new theories or models to explain the observed phenomenon. When a hypothesis is not supported by the data, it can help to refine existing theories or prompt the development of new hypotheses to explain the phenomenon.

When to use Hypothesis

Here are some common situations in which hypotheses are used:

  • In scientific research , hypotheses are used to guide the design of experiments and to help researchers make predictions about the outcomes of those experiments.
  • In social science research , hypotheses are used to test theories about human behavior, social relationships, and other phenomena.
  • I n business , hypotheses can be used to guide decisions about marketing, product development, and other areas. For example, a hypothesis might be that a new product will sell well in a particular market, and this hypothesis can be tested through market research.

Characteristics of Hypothesis

Here are some common characteristics of a hypothesis:

  • Testable : A hypothesis must be able to be tested through observation or experimentation. This means that it must be possible to collect data that will either support or refute the hypothesis.
  • Falsifiable : A hypothesis must be able to be proven false if it is not supported by the data. If a hypothesis cannot be falsified, then it is not a scientific hypothesis.
  • Clear and concise : A hypothesis should be stated in a clear and concise manner so that it can be easily understood and tested.
  • Based on existing knowledge : A hypothesis should be based on existing knowledge and research in the field. It should not be based on personal beliefs or opinions.
  • Specific : A hypothesis should be specific in terms of the variables being tested and the predicted outcome. This will help to ensure that the research is focused and well-designed.
  • Tentative: A hypothesis is a tentative statement or assumption that requires further testing and evidence to be confirmed or refuted. It is not a final conclusion or assertion.
  • Relevant : A hypothesis should be relevant to the research question or problem being studied. It should address a gap in knowledge or provide a new perspective on the issue.

Advantages of Hypothesis

Hypotheses have several advantages in scientific research and experimentation:

  • Guides research: A hypothesis provides a clear and specific direction for research. It helps to focus the research question, select appropriate methods and variables, and interpret the results.
  • Predictive powe r: A hypothesis makes predictions about the outcome of research, which can be tested through experimentation. This allows researchers to evaluate the validity of the hypothesis and make new discoveries.
  • Facilitates communication: A hypothesis provides a common language and framework for scientists to communicate with one another about their research. This helps to facilitate the exchange of ideas and promotes collaboration.
  • Efficient use of resources: A hypothesis helps researchers to use their time, resources, and funding efficiently by directing them towards specific research questions and methods that are most likely to yield results.
  • Provides a basis for further research: A hypothesis that is supported by data provides a basis for further research and exploration. It can lead to new hypotheses, theories, and discoveries.
  • Increases objectivity: A hypothesis can help to increase objectivity in research by providing a clear and specific framework for testing and interpreting results. This can reduce bias and increase the reliability of research findings.

Limitations of Hypothesis

Some Limitations of the Hypothesis are as follows:

  • Limited to observable phenomena: Hypotheses are limited to observable phenomena and cannot account for unobservable or intangible factors. This means that some research questions may not be amenable to hypothesis testing.
  • May be inaccurate or incomplete: Hypotheses are based on existing knowledge and research, which may be incomplete or inaccurate. This can lead to flawed hypotheses and erroneous conclusions.
  • May be biased: Hypotheses may be biased by the researcher’s own beliefs, values, or assumptions. This can lead to selective interpretation of data and a lack of objectivity in research.
  • Cannot prove causation: A hypothesis can only show a correlation between variables, but it cannot prove causation. This requires further experimentation and analysis.
  • Limited to specific contexts: Hypotheses are limited to specific contexts and may not be generalizable to other situations or populations. This means that results may not be applicable in other contexts or may require further testing.
  • May be affected by chance : Hypotheses may be affected by chance or random variation, which can obscure or distort the true relationship between variables.

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formulating a hypothesis scientific method

How to Write a Hypothesis: A Step-by-Step Guide

formulating a hypothesis scientific method

Introduction

An overview of the research hypothesis, different types of hypotheses, variables in a hypothesis, how to formulate an effective research hypothesis, designing a study around your hypothesis.

The scientific method can derive and test predictions as hypotheses. Empirical research can then provide support (or lack thereof) for the hypotheses. Even failure to find support for a hypothesis still represents a valuable contribution to scientific knowledge. Let's look more closely at the idea of the hypothesis and the role it plays in research.

formulating a hypothesis scientific method

As much as the term exists in everyday language, there is a detailed development that informs the word "hypothesis" when applied to research. A good research hypothesis is informed by prior research and guides research design and data analysis , so it is important to understand how a hypothesis is defined and understood by researchers.

What is the simple definition of a hypothesis?

A hypothesis is a testable prediction about an outcome between two or more variables . It functions as a navigational tool in the research process, directing what you aim to predict and how.

What is the hypothesis for in research?

In research, a hypothesis serves as the cornerstone for your empirical study. It not only lays out what you aim to investigate but also provides a structured approach for your data collection and analysis.

Essentially, it bridges the gap between the theoretical and the empirical, guiding your investigation throughout its course.

formulating a hypothesis scientific method

What is an example of a hypothesis?

If you are studying the relationship between physical exercise and mental health, a suitable hypothesis could be: "Regular physical exercise leads to improved mental well-being among adults."

This statement constitutes a specific and testable hypothesis that directly relates to the variables you are investigating.

What makes a good hypothesis?

A good hypothesis possesses several key characteristics. Firstly, it must be testable, allowing you to analyze data through empirical means, such as observation or experimentation, to assess if there is significant support for the hypothesis. Secondly, a hypothesis should be specific and unambiguous, giving a clear understanding of the expected relationship between variables. Lastly, it should be grounded in existing research or theoretical frameworks , ensuring its relevance and applicability.

Understanding the types of hypotheses can greatly enhance how you construct and work with hypotheses. While all hypotheses serve the essential function of guiding your study, there are varying purposes among the types of hypotheses. In addition, all hypotheses stand in contrast to the null hypothesis, or the assumption that there is no significant relationship between the variables .

Here, we explore various kinds of hypotheses to provide you with the tools needed to craft effective hypotheses for your specific research needs. Bear in mind that many of these hypothesis types may overlap with one another, and the specific type that is typically used will likely depend on the area of research and methodology you are following.

Null hypothesis

The null hypothesis is a statement that there is no effect or relationship between the variables being studied. In statistical terms, it serves as the default assumption that any observed differences are due to random chance.

For example, if you're studying the effect of a drug on blood pressure, the null hypothesis might state that the drug has no effect.

Alternative hypothesis

Contrary to the null hypothesis, the alternative hypothesis suggests that there is a significant relationship or effect between variables.

Using the drug example, the alternative hypothesis would posit that the drug does indeed affect blood pressure. This is what researchers aim to prove.

formulating a hypothesis scientific method

Simple hypothesis

A simple hypothesis makes a prediction about the relationship between two variables, and only two variables.

For example, "Increased study time results in better exam scores." Here, "study time" and "exam scores" are the only variables involved.

Complex hypothesis

A complex hypothesis, as the name suggests, involves more than two variables. For instance, "Increased study time and access to resources result in better exam scores." Here, "study time," "access to resources," and "exam scores" are all variables.

This hypothesis refers to multiple potential mediating variables. Other hypotheses could also include predictions about variables that moderate the relationship between the independent variable and dependent variable .

Directional hypothesis

A directional hypothesis specifies the direction of the expected relationship between variables. For example, "Eating more fruits and vegetables leads to a decrease in heart disease."

Here, the direction of heart disease is explicitly predicted to decrease, due to effects from eating more fruits and vegetables. All hypotheses typically specify the expected direction of the relationship between the independent and dependent variable, such that researchers can test if this prediction holds in their data analysis .

formulating a hypothesis scientific method

Statistical hypothesis

A statistical hypothesis is one that is testable through statistical methods, providing a numerical value that can be analyzed. This is commonly seen in quantitative research .

For example, "There is a statistically significant difference in test scores between students who study for one hour and those who study for two."

Empirical hypothesis

An empirical hypothesis is derived from observations and is tested through empirical methods, often through experimentation or survey data . Empirical hypotheses may also be assessed with statistical analyses.

For example, "Regular exercise is correlated with a lower incidence of depression," could be tested through surveys that measure exercise frequency and depression levels.

Causal hypothesis

A causal hypothesis proposes that one variable causes a change in another. This type of hypothesis is often tested through controlled experiments.

For example, "Smoking causes lung cancer," assumes a direct causal relationship.

Associative hypothesis

Unlike causal hypotheses, associative hypotheses suggest a relationship between variables but do not imply causation.

For instance, "People who smoke are more likely to get lung cancer," notes an association but doesn't claim that smoking causes lung cancer directly.

Relational hypothesis

A relational hypothesis explores the relationship between two or more variables but doesn't specify the nature of the relationship.

For example, "There is a relationship between diet and heart health," leaves the nature of the relationship (causal, associative, etc.) open to interpretation.

Logical hypothesis

A logical hypothesis is based on sound reasoning and logical principles. It's often used in theoretical research to explore abstract concepts, rather than being based on empirical data.

For example, "If all men are mortal and Socrates is a man, then Socrates is mortal," employs logical reasoning to make its point.

formulating a hypothesis scientific method

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In any research hypothesis, variables play a critical role. These are the elements or factors that the researcher manipulates, controls, or measures. Understanding variables is essential for crafting a clear, testable hypothesis and for the stages of research that follow, such as data collection and analysis.

In the realm of hypotheses, there are generally two types of variables to consider: independent and dependent. Independent variables are what you, as the researcher, manipulate or change in your study. It's considered the cause in the relationship you're investigating. For instance, in a study examining the impact of sleep duration on academic performance, the independent variable would be the amount of sleep participants get.

Conversely, the dependent variable is the outcome you measure to gauge the effect of your manipulation. It's the effect in the cause-and-effect relationship. The dependent variable thus refers to the main outcome of interest in your study. In the same sleep study example, the academic performance, perhaps measured by exam scores or GPA, would be the dependent variable.

Beyond these two primary types, you might also encounter control variables. These are variables that could potentially influence the outcome and are therefore kept constant to isolate the relationship between the independent and dependent variables . For example, in the sleep and academic performance study, control variables could include age, diet, or even the subject of study.

By clearly identifying and understanding the roles of these variables in your hypothesis, you set the stage for a methodologically sound research project. It helps you develop focused research questions, design appropriate experiments or observations, and carry out meaningful data analysis . It's a step that lays the groundwork for the success of your entire study.

formulating a hypothesis scientific method

Crafting a strong, testable hypothesis is crucial for the success of any research project. It sets the stage for everything from your study design to data collection and analysis . Below are some key considerations to keep in mind when formulating your hypothesis:

  • Be specific : A vague hypothesis can lead to ambiguous results and interpretations . Clearly define your variables and the expected relationship between them.
  • Ensure testability : A good hypothesis should be testable through empirical means, whether by observation , experimentation, or other forms of data analysis.
  • Ground in literature : Before creating your hypothesis, consult existing research and theories. This not only helps you identify gaps in current knowledge but also gives you valuable context and credibility for crafting your hypothesis.
  • Use simple language : While your hypothesis should be conceptually sound, it doesn't have to be complicated. Aim for clarity and simplicity in your wording.
  • State direction, if applicable : If your hypothesis involves a directional outcome (e.g., "increase" or "decrease"), make sure to specify this. You also need to think about how you will measure whether or not the outcome moved in the direction you predicted.
  • Keep it focused : One of the common pitfalls in hypothesis formulation is trying to answer too many questions at once. Keep your hypothesis focused on a specific issue or relationship.
  • Account for control variables : Identify any variables that could potentially impact the outcome and consider how you will control for them in your study.
  • Be ethical : Make sure your hypothesis and the methods for testing it comply with ethical standards , particularly if your research involves human or animal subjects.

formulating a hypothesis scientific method

Designing your study involves multiple key phases that help ensure the rigor and validity of your research. Here we discuss these crucial components in more detail.

Literature review

Starting with a comprehensive literature review is essential. This step allows you to understand the existing body of knowledge related to your hypothesis and helps you identify gaps that your research could fill. Your research should aim to contribute some novel understanding to existing literature, and your hypotheses can reflect this. A literature review also provides valuable insights into how similar research projects were executed, thereby helping you fine-tune your own approach.

formulating a hypothesis scientific method

Research methods

Choosing the right research methods is critical. Whether it's a survey, an experiment, or observational study, the methodology should be the most appropriate for testing your hypothesis. Your choice of methods will also depend on whether your research is quantitative, qualitative, or mixed-methods. Make sure the chosen methods align well with the variables you are studying and the type of data you need.

Preliminary research

Before diving into a full-scale study, it’s often beneficial to conduct preliminary research or a pilot study . This allows you to test your research methods on a smaller scale, refine your tools, and identify any potential issues. For instance, a pilot survey can help you determine if your questions are clear and if the survey effectively captures the data you need. This step can save you both time and resources in the long run.

Data analysis

Finally, planning your data analysis in advance is crucial for a successful study. Decide which statistical or analytical tools are most suited for your data type and research questions . For quantitative research, you might opt for t-tests, ANOVA, or regression analyses. For qualitative research , thematic analysis or grounded theory may be more appropriate. This phase is integral for interpreting your results and drawing meaningful conclusions in relation to your research question.

formulating a hypothesis scientific method

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formulating a hypothesis scientific method

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How to Write a Great Hypothesis

Hypothesis Format, Examples, and Tips

Kendra Cherry, MS, is a psychosocial rehabilitation specialist, psychology educator, and author of the "Everything Psychology Book."

formulating a hypothesis scientific method

Amy Morin, LCSW, is a psychotherapist and international bestselling author. Her books, including "13 Things Mentally Strong People Don't Do," have been translated into more than 40 languages. Her TEDx talk,  "The Secret of Becoming Mentally Strong," is one of the most viewed talks of all time.

formulating a hypothesis scientific method

Verywell / Alex Dos Diaz

  • The Scientific Method

Hypothesis Format

Falsifiability of a hypothesis, operational definitions, types of hypotheses, hypotheses examples.

  • Collecting Data

Frequently Asked Questions

A hypothesis is a tentative statement about the relationship between two or more  variables. It is a specific, testable prediction about what you expect to happen in a study.

One hypothesis example would be a study designed to look at the relationship between sleep deprivation and test performance might have a hypothesis that states: "This study is designed to assess the hypothesis that sleep-deprived people will perform worse on a test than individuals who are not sleep-deprived."

This article explores how a hypothesis is used in psychology research, how to write a good hypothesis, and the different types of hypotheses you might use.

The Hypothesis in the Scientific Method

In the scientific method , whether it involves research in psychology, biology, or some other area, a hypothesis represents what the researchers think will happen in an experiment. The scientific method involves the following steps:

  • Forming a question
  • Performing background research
  • Creating a hypothesis
  • Designing an experiment
  • Collecting data
  • Analyzing the results
  • Drawing conclusions
  • Communicating the results

The hypothesis is a prediction, but it involves more than a guess. Most of the time, the hypothesis begins with a question which is then explored through background research. It is only at this point that researchers begin to develop a testable hypothesis. Unless you are creating an exploratory study, your hypothesis should always explain what you  expect  to happen.

In a study exploring the effects of a particular drug, the hypothesis might be that researchers expect the drug to have some type of effect on the symptoms of a specific illness. In psychology, the hypothesis might focus on how a certain aspect of the environment might influence a particular behavior.

Remember, a hypothesis does not have to be correct. While the hypothesis predicts what the researchers expect to see, the goal of the research is to determine whether this guess is right or wrong. When conducting an experiment, researchers might explore a number of factors to determine which ones might contribute to the ultimate outcome.

In many cases, researchers may find that the results of an experiment  do not  support the original hypothesis. When writing up these results, the researchers might suggest other options that should be explored in future studies.

In many cases, researchers might draw a hypothesis from a specific theory or build on previous research. For example, prior research has shown that stress can impact the immune system. So a researcher might hypothesize: "People with high-stress levels will be more likely to contract a common cold after being exposed to the virus than people who have low-stress levels."

In other instances, researchers might look at commonly held beliefs or folk wisdom. "Birds of a feather flock together" is one example of folk wisdom that a psychologist might try to investigate. The researcher might pose a specific hypothesis that "People tend to select romantic partners who are similar to them in interests and educational level."

Elements of a Good Hypothesis

So how do you write a good hypothesis? When trying to come up with a hypothesis for your research or experiments, ask yourself the following questions:

  • Is your hypothesis based on your research on a topic?
  • Can your hypothesis be tested?
  • Does your hypothesis include independent and dependent variables?

Before you come up with a specific hypothesis, spend some time doing background research. Once you have completed a literature review, start thinking about potential questions you still have. Pay attention to the discussion section in the  journal articles you read . Many authors will suggest questions that still need to be explored.

To form a hypothesis, you should take these steps:

  • Collect as many observations about a topic or problem as you can.
  • Evaluate these observations and look for possible causes of the problem.
  • Create a list of possible explanations that you might want to explore.
  • After you have developed some possible hypotheses, think of ways that you could confirm or disprove each hypothesis through experimentation. This is known as falsifiability.

In the scientific method ,  falsifiability is an important part of any valid hypothesis.   In order to test a claim scientifically, it must be possible that the claim could be proven false.

Students sometimes confuse the idea of falsifiability with the idea that it means that something is false, which is not the case. What falsifiability means is that  if  something was false, then it is possible to demonstrate that it is false.

One of the hallmarks of pseudoscience is that it makes claims that cannot be refuted or proven false.

A variable is a factor or element that can be changed and manipulated in ways that are observable and measurable. However, the researcher must also define how the variable will be manipulated and measured in the study.

For example, a researcher might operationally define the variable " test anxiety " as the results of a self-report measure of anxiety experienced during an exam. A "study habits" variable might be defined by the amount of studying that actually occurs as measured by time.

These precise descriptions are important because many things can be measured in a number of different ways. One of the basic principles of any type of scientific research is that the results must be replicable.   By clearly detailing the specifics of how the variables were measured and manipulated, other researchers can better understand the results and repeat the study if needed.

Some variables are more difficult than others to define. How would you operationally define a variable such as aggression ? For obvious ethical reasons, researchers cannot create a situation in which a person behaves aggressively toward others.

In order to measure this variable, the researcher must devise a measurement that assesses aggressive behavior without harming other people. In this situation, the researcher might utilize a simulated task to measure aggressiveness.

Hypothesis Checklist

  • Does your hypothesis focus on something that you can actually test?
  • Does your hypothesis include both an independent and dependent variable?
  • Can you manipulate the variables?
  • Can your hypothesis be tested without violating ethical standards?

The hypothesis you use will depend on what you are investigating and hoping to find. Some of the main types of hypotheses that you might use include:

  • Simple hypothesis : This type of hypothesis suggests that there is a relationship between one independent variable and one dependent variable.
  • Complex hypothesis : This type of hypothesis suggests a relationship between three or more variables, such as two independent variables and a dependent variable.
  • Null hypothesis : This hypothesis suggests no relationship exists between two or more variables.
  • Alternative hypothesis : This hypothesis states the opposite of the null hypothesis.
  • Statistical hypothesis : This hypothesis uses statistical analysis to evaluate a representative sample of the population and then generalizes the findings to the larger group.
  • Logical hypothesis : This hypothesis assumes a relationship between variables without collecting data or evidence.

A hypothesis often follows a basic format of "If {this happens} then {this will happen}." One way to structure your hypothesis is to describe what will happen to the  dependent variable  if you change the  independent variable .

The basic format might be: "If {these changes are made to a certain independent variable}, then we will observe {a change in a specific dependent variable}."

A few examples of simple hypotheses:

  • "Students who eat breakfast will perform better on a math exam than students who do not eat breakfast."
  • Complex hypothesis: "Students who experience test anxiety before an English exam will get lower scores than students who do not experience test anxiety."​
  • "Motorists who talk on the phone while driving will be more likely to make errors on a driving course than those who do not talk on the phone."

Examples of a complex hypothesis include:

  • "People with high-sugar diets and sedentary activity levels are more likely to develop depression."
  • "Younger people who are regularly exposed to green, outdoor areas have better subjective well-being than older adults who have limited exposure to green spaces."

Examples of a null hypothesis include:

  • "Children who receive a new reading intervention will have scores different than students who do not receive the intervention."
  • "There will be no difference in scores on a memory recall task between children and adults."

Examples of an alternative hypothesis:

  • "Children who receive a new reading intervention will perform better than students who did not receive the intervention."
  • "Adults will perform better on a memory task than children." 

Collecting Data on Your Hypothesis

Once a researcher has formed a testable hypothesis, the next step is to select a research design and start collecting data. The research method depends largely on exactly what they are studying. There are two basic types of research methods: descriptive research and experimental research.

Descriptive Research Methods

Descriptive research such as  case studies ,  naturalistic observations , and surveys are often used when it would be impossible or difficult to  conduct an experiment . These methods are best used to describe different aspects of a behavior or psychological phenomenon.

Once a researcher has collected data using descriptive methods, a correlational study can then be used to look at how the variables are related. This type of research method might be used to investigate a hypothesis that is difficult to test experimentally.

Experimental Research Methods

Experimental methods  are used to demonstrate causal relationships between variables. In an experiment, the researcher systematically manipulates a variable of interest (known as the independent variable) and measures the effect on another variable (known as the dependent variable).

Unlike correlational studies, which can only be used to determine if there is a relationship between two variables, experimental methods can be used to determine the actual nature of the relationship—whether changes in one variable actually  cause  another to change.

A Word From Verywell

The hypothesis is a critical part of any scientific exploration. It represents what researchers expect to find in a study or experiment. In situations where the hypothesis is unsupported by the research, the research still has value. Such research helps us better understand how different aspects of the natural world relate to one another. It also helps us develop new hypotheses that can then be tested in the future.

Some examples of how to write a hypothesis include:

  • "Staying up late will lead to worse test performance the next day."
  • "People who consume one apple each day will visit the doctor fewer times each year."
  • "Breaking study sessions up into three 20-minute sessions will lead to better test results than a single 60-minute study session."

The four parts of a hypothesis are:

  • The research question
  • The independent variable (IV)
  • The dependent variable (DV)
  • The proposed relationship between the IV and DV

Castillo M. The scientific method: a need for something better? . AJNR Am J Neuroradiol. 2013;34(9):1669-71. doi:10.3174/ajnr.A3401

Nevid J. Psychology: Concepts and Applications. Wadworth, 2013.

By Kendra Cherry, MSEd Kendra Cherry, MS, is a psychosocial rehabilitation specialist, psychology educator, and author of the "Everything Psychology Book."

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2.4C: Formulating the Hypothesis

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A hypothesis is a potential answer to your research question; the research process helps you determine if your hypothesis is true.

Learning Objectives

  • Explain how hypotheses are used in sociological research and the difference between dependent and independent variables
  • Hypotheses are testable explanations of a problem, phenomenon, or observation.
  • Both quantitative and qualitative research involve formulating a hypothesis to address the research problem.
  • Hypotheses that suggest a causal relationship involve at least one independent variable and at least one dependent variable; in other words, one variable which is presumed to affect the other.
  • An independent variable is one whose value is manipulated by the researcher or experimenter.
  • A dependent variable is a variable whose values are presumed to change as a result of changes in the independent variable.
  • dependent variable : In an equation, the variable whose value depends on one or more variables in the equation.
  • independent variable : In an equation, any variable whose value is not dependent on any other in the equation.
  • hypothesis : Used loosely, a tentative conjecture explaining an observation, phenomenon, or scientific problem that can be tested by further observation, investigation, or experimentation.

A hypothesis is an assumption or suggested explanation about how two or more variables are related. It is a crucial step in the scientific method and, therefore, a vital aspect of all scientific research. There are no definitive guidelines for the production of new hypotheses. The history of science is filled with stories of scientists claiming a flash of inspiration, or a hunch, which then motivated them to look for evidence to support or refute the idea.

image

While there is no single way to develop a hypothesis, a useful hypothesis will use deductive reasoning to make predictions that can be experimentally assessed. If results contradict the predictions, then the hypothesis under examination is incorrect or incomplete and must be revised or abandoned. If results confirm the predictions, then the hypothesis might be correct but is still subject to further testing.

Both quantitative and qualitative research involve formulating a hypothesis to address the research problem. A hypothesis will generally provide a causal explanation or propose some association between two variables. Variables are measurable phenomena whose values can change under different conditions. For example, if the hypothesis is a causal explanation, it will involve at least one dependent variable and one independent variable. In research, independent variables are the cause of the change. The dependent variable is the effect, or thing that is changed. In other words, the value of a dependent variable depends on the value of the independent variable. Of course, this assumes that there is an actual relationship between the two variables. If there is no relationship, then the value of the dependent variable does not depend on the value of the independent variable.

What Are The Steps Of The Scientific Method?

Julia Simkus

Editor at Simply Psychology

BA (Hons) Psychology, Princeton University

Julia Simkus is a graduate of Princeton University with a Bachelor of Arts in Psychology. She is currently studying for a Master's Degree in Counseling for Mental Health and Wellness in September 2023. Julia's research has been published in peer reviewed journals.

Learn about our Editorial Process

Saul Mcleod, PhD

Editor-in-Chief for Simply Psychology

BSc (Hons) Psychology, MRes, PhD, University of Manchester

Saul Mcleod, PhD., is a qualified psychology teacher with over 18 years of experience in further and higher education. He has been published in peer-reviewed journals, including the Journal of Clinical Psychology.

Olivia Guy-Evans, MSc

Associate Editor for Simply Psychology

BSc (Hons) Psychology, MSc Psychology of Education

Olivia Guy-Evans is a writer and associate editor for Simply Psychology. She has previously worked in healthcare and educational sectors.

On This Page:

Science is not just knowledge. It is also a method for obtaining knowledge. Scientific understanding is organized into theories.

The scientific method is a step-by-step process used by researchers and scientists to determine if there is a relationship between two or more variables. Psychologists use this method to conduct psychological research, gather data, process information, and describe behaviors.

It involves careful observation, asking questions, formulating hypotheses, experimental testing, and refining hypotheses based on experimental findings.

How it is Used

The scientific method can be applied broadly in science across many different fields, such as chemistry, physics, geology, and psychology. In a typical application of this process, a researcher will develop a hypothesis, test this hypothesis, and then modify the hypothesis based on the outcomes of the experiment.

The process is then repeated with the modified hypothesis until the results align with the observed phenomena. Detailed steps of the scientific method are described below.

Keep in mind that the scientific method does not have to follow this fixed sequence of steps; rather, these steps represent a set of general principles or guidelines.

7 Steps of the Scientific Method

Psychology uses an empirical approach.

Empiricism (founded by John Locke) states that the only source of knowledge comes through our senses – e.g., sight, hearing, touch, etc.

Empirical evidence does not rely on argument or belief. Thus, empiricism is the view that all knowledge is based on or may come from direct observation and experience.

The empiricist approach of gaining knowledge through experience quickly became the scientific approach and greatly influenced the development of physics and chemistry in the 17th and 18th centuries.

Steps of the Scientific Method

Step 1: Make an Observation (Theory Construction)

Every researcher starts at the very beginning. Before diving in and exploring something, one must first determine what they will study – it seems simple enough!

By making observations, researchers can establish an area of interest. Once this topic of study has been chosen, a researcher should review existing literature to gain insight into what has already been tested and determine what questions remain unanswered.

This assessment will provide helpful information about what has already been comprehended about the specific topic and what questions remain, and if one can go and answer them.

Specifically, a literature review might implicate examining a substantial amount of documented material from academic journals to books dating back decades. The most appropriate information gathered by the researcher will be shown in the introduction section or abstract of the published study results.

The background material and knowledge will help the researcher with the first significant step in conducting a psychology study, which is formulating a research question.

This is the inductive phase of the scientific process. Observations yield information that is used to formulate theories as explanations. A theory is a well-developed set of ideas that propose an explanation for observed phenomena.

Inductive reasoning moves from specific premises to a general conclusion. It starts with observations of phenomena in the natural world and derives a general law.

Step 2: Ask a Question

Once a researcher has made observations and conducted background research, the next step is to ask a scientific question. A scientific question must be defined, testable, and measurable.

A useful approach to develop a scientific question is: “What is the effect of…?” or “How does X affect Y?”

To answer an experimental question, a researcher must identify two variables: the independent and dependent variables.

The independent variable is the variable manipulated (the cause), and the dependent variable is the variable being measured (the effect).

An example of a research question could be, “Is handwriting or typing more effective for retaining information?” Answering the research question and proposing a relationship between the two variables is discussed in the next step.

Step 3: Form a Hypothesis (Make Predictions)

A hypothesis is an educated guess about the relationship between two or more variables. A hypothesis is an attempt to answer your research question based on prior observation and background research. Theories tend to be too complex to be tested all at once; instead, researchers create hypotheses to test specific aspects of a theory.

For example, a researcher might ask about the connection between sleep and educational performance. Do students who get less sleep perform worse on tests at school?

It is crucial to think about different questions one might have about a particular topic to formulate a reasonable hypothesis. It would help if one also considered how one could investigate the causalities.

It is important that the hypothesis is both testable against reality and falsifiable. This means that it can be tested through an experiment and can be proven wrong.

The falsification principle, proposed by Karl Popper , is a way of demarcating science from non-science. It suggests that for a theory to be considered scientific, it must be able to be tested and conceivably proven false.

To test a hypothesis, we first assume that there is no difference between the populations from which the samples were taken. This is known as the null hypothesis and predicts that the independent variable will not influence the dependent variable.

Examples of “if…then…” Hypotheses:

  • If one gets less than 6 hours of sleep, then one will do worse on tests than if one obtains more rest.
  • If one drinks lots of water before going to bed, one will have to use the bathroom often at night.
  • If one practices exercising and lighting weights, then one’s body will begin to build muscle.

The research hypothesis is often called the alternative hypothesis and predicts what change(s) will occur in the dependent variable when the independent variable is manipulated.

It states that the results are not due to chance and that they are significant in terms of supporting the theory being investigated.

Although one could state and write a scientific hypothesis in many ways, hypotheses are usually built like “if…then…” statements.

Step 4: Run an Experiment (Gather Data)

The next step in the scientific method is to test your hypothesis and collect data. A researcher will design an experiment to test the hypothesis and gather data that will either support or refute the hypothesis.

The exact research methods used to examine a hypothesis depend on what is being studied. A psychologist might utilize two primary forms of research, experimental research, and descriptive research.

The scientific method is objective in that researchers do not let preconceived ideas or biases influence the collection of data and is systematic in that experiments are conducted in a logical way.

Experimental Research

Experimental research is used to investigate cause-and-effect associations between two or more variables. This type of research systematically controls an independent variable and measures its effect on a specified dependent variable.

Experimental research involves manipulating an independent variable and measuring the effect(s) on the dependent variable. Repeating the experiment multiple times is important to confirm that your results are accurate and consistent.

One of the significant advantages of this method is that it permits researchers to determine if changes in one variable cause shifts in each other.

While experiments in psychology typically have many moving parts (and can be relatively complex), an easy investigation is rather fundamental. Still, it does allow researchers to specify cause-and-effect associations between variables.

Most simple experiments use a control group, which involves those who do not receive the treatment, and an experimental group, which involves those who do receive the treatment.

An example of experimental research would be when a pharmaceutical company wants to test a new drug. They give one group a placebo (control group) and the other the actual pill (experimental group).

Descriptive Research

Descriptive research is generally used when it is challenging or even impossible to control the variables in question. Examples of descriptive analysis include naturalistic observation, case studies , and correlation studies .

One example of descriptive research includes phone surveys that marketers often use. While they typically do not allow researchers to identify cause and effect, correlational studies are quite common in psychology research. They make it possible to spot associations between distinct variables and measure the solidity of those relationships.

Step 5: Analyze the Data and Draw Conclusions

Once a researcher has designed and done the investigation and collected sufficient data, it is time to inspect this gathered information and judge what has been found. Researchers can summarize the data, interpret the results, and draw conclusions based on this evidence using analyses and statistics.

Upon completion of the experiment, you can collect your measurements and analyze the data using statistics. Based on the outcomes, you will either reject or confirm your hypothesis.

Analyze the Data

So, how does a researcher determine what the results of their study mean? Statistical analysis can either support or refute a researcher’s hypothesis and can also be used to determine if the conclusions are statistically significant.

When outcomes are said to be “statistically significant,” it is improbable that these results are due to luck or chance. Based on these observations, investigators must then determine what the results mean.

An experiment will support a hypothesis in some circumstances, but sometimes it fails to be truthful in other cases.

What occurs if the developments of a psychology investigation do not endorse the researcher’s hypothesis? It does mean that the study was worthless. Simply because the findings fail to defend the researcher’s hypothesis does not mean that the examination is not helpful or instructive.

This kind of research plays a vital role in supporting scientists in developing unexplored questions and hypotheses to investigate in the future. After decisions have been made, the next step is to communicate the results with the rest of the scientific community.

This is an integral part of the process because it contributes to the general knowledge base and can assist other scientists in finding new research routes to explore.

If the hypothesis is not supported, a researcher should acknowledge the experiment’s results, formulate a new hypothesis, and develop a new experiment.

We must avoid any reference to results proving a theory as this implies 100% certainty, and there is always a chance that evidence may exist that could refute a theory.

Draw Conclusions and Interpret the Data

When the empirical observations disagree with the hypothesis, a number of possibilities must be considered. It might be that the theory is incorrect, in which case it needs altering, so it fully explains the data.

Alternatively, it might be that the hypothesis was poorly derived from the original theory, in which case the scientists were expecting the wrong thing to happen.

It might also be that the research was poorly conducted, or used an inappropriate method, or there were factors in play that the researchers did not consider. This will begin the process of the scientific method again.

If the hypothesis is supported, the researcher can find more evidence to support their hypothesis or look for counter-evidence to strengthen their hypothesis further.

In either scenario, the researcher should share their results with the greater scientific community.

Step 6: Share Your Results

One of the final stages of the research cycle involves the publication of the research. Once the report is written, the researcher(s) may submit the work for publication in an appropriate journal.

Usually, this is done by writing up a study description and publishing the article in a professional or academic journal. The studies and conclusions of psychological work can be seen in peer-reviewed journals such as  Developmental Psychology , Psychological Bulletin, the  Journal of Social Psychology, and numerous others.

Scientists should report their findings by writing up a description of their study and any subsequent findings. This enables other researchers to build upon the present research or replicate the results.

As outlined by the American Psychological Association (APA), there is a typical structure of a journal article that follows a specified format. In these articles, researchers:

  • Supply a brief narrative and background on previous research
  • Give their hypothesis
  • Specify who participated in the study and how they were chosen
  • Provide operational definitions for each variable
  • Explain the measures and methods used to collect data
  • Describe how the data collected was interpreted
  • Discuss what the outcomes mean

A detailed record of psychological studies and all scientific studies is vital to clearly explain the steps and procedures used throughout the study. So that other researchers can try this experiment too and replicate the results.

The editorial process utilized by academic and professional journals guarantees that each submitted article undergoes a thorough peer review to help assure that the study is scientifically sound. Once published, the investigation becomes another piece of the current puzzle of our knowledge “base” on that subject.

This last step is important because all results, whether they supported or did not support the hypothesis, can contribute to the scientific community. Publication of empirical observations leads to more ideas that are tested against the real world, and so on. In this sense, the scientific process is circular.

The editorial process utilized by academic and professional journals guarantees that each submitted article undergoes a thorough peer review to help assure that the study is scientifically sound.

Once published, the investigation becomes another piece of the current puzzle of our knowledge “base” on that subject.

By replicating studies, psychologists can reduce errors, validate theories, and gain a stronger understanding of a particular topic.

Step 7: Repeat the Scientific Method (Iteration)

Now, if one’s hypothesis turns out to be accurate, find more evidence or find counter-evidence. If one’s hypothesis is false, create a new hypothesis or try again.

One may wish to revise their first hypothesis to make a more niche experiment to design or a different specific question to test.

The amazingness of the scientific method is that it is a comprehensive and straightforward process that scientists, and everyone, can utilize over and over again.

So, draw conclusions and repeat because the scientific method is never-ending, and no result is ever considered perfect.

The scientific method is a process of:

  • Making an observation.
  • Forming a hypothesis.
  • Making a prediction.
  • Experimenting to test the hypothesis.

The procedure of repeating the scientific method is crucial to science and all fields of human knowledge.

Further Information

  • Karl Popper – Falsification
  • Thomas – Kuhn Paradigm Shift
  • Positivism in Sociology: Definition, Theory & Examples
  • Is Psychology a Science?
  • Psychology as a Science (PDF)

List the 6 steps of the scientific methods in order

  • Make an observation (theory construction)
  • Ask a question. A scientific question must be defined, testable, and measurable.
  • Form a hypothesis (make predictions)
  • Run an experiment to test the hypothesis (gather data)
  • Analyze the data and draw conclusions
  • Share your results so that other researchers can make new hypotheses

What is the first step of the scientific method?

The first step of the scientific method is making an observation. This involves noticing and describing a phenomenon or group of phenomena that one finds interesting and wishes to explain.

Observations can occur in a natural setting or within the confines of a laboratory. The key point is that the observation provides the initial question or problem that the rest of the scientific method seeks to answer or solve.

What is the scientific method?

The scientific method is a step-by-step process that investigators can follow to determine if there is a causal connection between two or more variables.

Psychologists and other scientists regularly suggest motivations for human behavior. On a more casual level, people judge other people’s intentions, incentives, and actions daily.

While our standard assessments of human behavior are subjective and anecdotal, researchers use the scientific method to study psychology objectively and systematically.

All utilize a scientific method to study distinct aspects of people’s thinking and behavior. This process allows scientists to analyze and understand various psychological phenomena, but it also provides investigators and others a way to disseminate and debate the results of their studies.

The outcomes of these studies are often noted in popular media, which leads numerous to think about how or why researchers came to the findings they did.

Why Use the Six Steps of the Scientific Method

The goal of scientists is to understand better the world that surrounds us. Scientific research is the most critical tool for navigating and learning about our complex world.

Without it, we would be compelled to rely solely on intuition, other people’s power, and luck. We can eliminate our preconceived concepts and superstitions through methodical scientific research and gain an objective sense of ourselves and our world.

All psychological studies aim to explain, predict, and even control or impact mental behaviors or processes. So, psychologists use and repeat the scientific method (and its six steps) to perform and record essential psychological research.

So, psychologists focus on understanding behavior and the cognitive (mental) and physiological (body) processes underlying behavior.

In the real world, people use to understand the behavior of others, such as intuition and personal experience. The hallmark of scientific research is evidence to support a claim.

Scientific knowledge is empirical, meaning it is grounded in objective, tangible evidence that can be observed repeatedly, regardless of who is watching.

The scientific method is crucial because it minimizes the impact of bias or prejudice on the experimenter. Regardless of how hard one tries, even the best-intentioned scientists can’t escape discrimination. can’t

It stems from personal opinions and cultural beliefs, meaning any mortal filters data based on one’s experience. Sadly, this “filtering” process can cause a scientist to favor one outcome over another.

For an everyday person trying to solve a minor issue at home or work, succumbing to these biases is not such a big deal; in fact, most times, it is important.

But in the scientific community, where results must be inspected and reproduced, bias or discrimination must be avoided.

When to Use the Six Steps of the Scientific Method ?

One can use the scientific method anytime, anywhere! From the smallest conundrum to solving global problems, it is a process that can be applied to any science and any investigation.

Even if you are not considered a “scientist,” you will be surprised to know that people of all disciplines use it for all kinds of dilemmas.

Try to catch yourself next time you come by a question and see how you subconsciously or consciously use the scientific method.

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Perspective: Dimensions of the scientific method

Eberhard o. voit.

Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, Georgia, United States of America

The scientific method has been guiding biological research for a long time. It not only prescribes the order and types of activities that give a scientific study validity and a stamp of approval but also has substantially shaped how we collectively think about the endeavor of investigating nature. The advent of high-throughput data generation, data mining, and advanced computational modeling has thrown the formerly undisputed, monolithic status of the scientific method into turmoil. On the one hand, the new approaches are clearly successful and expect the same acceptance as the traditional methods, but on the other hand, they replace much of the hypothesis-driven reasoning with inductive argumentation, which philosophers of science consider problematic. Intrigued by the enormous wealth of data and the power of machine learning, some scientists have even argued that significant correlations within datasets could make the entire quest for causation obsolete. Many of these issues have been passionately debated during the past two decades, often with scant agreement. It is proffered here that hypothesis-driven, data-mining–inspired, and “allochthonous” knowledge acquisition, based on mathematical and computational models, are vectors spanning a 3D space of an expanded scientific method. The combination of methods within this space will most certainly shape our thinking about nature, with implications for experimental design, peer review and funding, sharing of result, education, medical diagnostics, and even questions of litigation.

The traditional scientific method: Hypothesis-driven deduction

Research is the undisputed core activity defining science. Without research, the advancement of scientific knowledge would come to a screeching halt. While it is evident that researchers look for new information or insights, the term “research” is somewhat puzzling. Never mind the prefix “re,” which simply means “coming back and doing it again and again,” the word “search” seems to suggest that the research process is somewhat haphazard, that not much of a strategy is involved in the process. One might argue that research a few hundred years ago had the character of hoping for enough luck to find something new. The alchemists come to mind in their quest to turn mercury or lead into gold, or to discover an elixir for eternal youth, through methods we nowadays consider laughable.

Today’s sciences, in stark contrast, are clearly different. Yes, we still try to find something new—and may need a good dose of luck—but the process is anything but unstructured. In fact, it is prescribed in such rigor that it has been given the widely known moniker “scientific method.” This scientific method has deep roots going back to Aristotle and Herophilus (approximately 300 BC), Avicenna and Alhazen (approximately 1,000 AD), Grosseteste and Robert Bacon (approximately 1,250 AD), and many others, but solidified and crystallized into the gold standard of quality research during the 17th and 18th centuries [ 1 – 7 ]. In particular, Sir Francis Bacon (1561–1626) and René Descartes (1596–1650) are often considered the founders of the scientific method, because they insisted on careful, systematic observations of high quality, rather than metaphysical speculations that were en vogue among the scholars of the time [ 1 , 8 ]. In contrast to their peers, they strove for objectivity and insisted that observations, rather than an investigator’s preconceived ideas or superstitions, should be the basis for formulating a research idea [ 7 , 9 ].

Bacon and his 19th century follower John Stuart Mill explicitly proposed gaining knowledge through inductive reasoning: Based on carefully recorded observations, or from data obtained in a well-planned experiment, generalized assertions were to be made about similar yet (so far) unobserved phenomena [ 7 ]. Expressed differently, inductive reasoning attempts to derive general principles or laws directly from empirical evidence [ 10 ]. An example is the 19th century epigram of the physician Rudolf Virchow, Omnis cellula e cellula . There is no proof that indeed “every cell derives from a cell,” but like Virchow, we have made the observation time and again and never encountered anything suggesting otherwise.

In contrast to induction, the widely accepted, traditional scientific method is based on formulating and testing hypotheses. From the results of these tests, a deduction is made whether the hypothesis is presumably true or false. This type of hypotheticodeductive reasoning goes back to William Whewell, William Stanley Jevons, and Charles Peirce in the 19th century [ 1 ]. By the 20th century, the deductive, hypothesis-based scientific method had become deeply ingrained in the scientific psyche, and it is now taught as early as middle school in order to teach students valid means of discovery [ 8 , 11 , 12 ]. The scientific method has not only guided most research studies but also fundamentally influenced how we think about the process of scientific discovery.

Alas, because biology has almost no general laws, deduction in the strictest sense is difficult. It may therefore be preferable to use the term abduction, which refers to the logical inference toward the most plausible explanation, given a set of observations, although this explanation cannot be proven and is not necessarily true.

Over the decades, the hypothesis-based scientific method did experience variations here and there, but its conceptual scaffold remained essentially unchanged ( Fig 1 ). Its key is a process that begins with the formulation of a hypothesis that is to be rigorously tested, either in the wet lab or computationally; nonadherence to this principle is seen as lacking rigor and can lead to irreproducible results [ 1 , 13 – 15 ].

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Object name is pcbi.1007279.g001.jpg

The central concept of the traditional scientific method is a falsifiable hypothesis regarding some phenomenon of interest. This hypothesis is to be tested experimentally or computationally. The test results support or refute the hypothesis, triggering a new round of hypothesis formulation and testing.

Going further, the prominent philosopher of science Sir Karl Popper argued that a scientific hypothesis can never be verified but that it can be disproved by a single counterexample. He therefore demanded that scientific hypotheses had to be falsifiable, because otherwise, testing would be moot [ 16 , 17 ] (see also [ 18 ]). As Gillies put it, “successful theories are those that survive elimination through falsification” [ 19 ]. Kelley and Scott agreed to some degree but warned that complete insistence on falsifiability is too restrictive as it would mark many computational techniques, statistical hypothesis testing, and even Darwin’s theory of evolution as nonscientific [ 20 ].

While the hypothesis-based scientific method has been very successful, its exclusive reliance on deductive reasoning is dangerous because according to the so-called Duhem–Quine thesis, hypothesis testing always involves an unknown number of explicit or implicit assumptions, some of which may steer the researcher away from hypotheses that seem implausible, although they are, in fact, true [ 21 ]. According to Kuhn, this bias can obstruct the recognition of paradigm shifts [ 22 ], which require the rethinking of previously accepted “truths” and the development of radically new ideas [ 23 , 24 ]. The testing of simultaneous alternative hypotheses [ 25 – 27 ] ameliorates this problem to some degree but not entirely.

The traditional scientific method is often presented in discrete steps, but it should really be seen as a form of critical thinking, subject to review and independent validation [ 8 ]. It has proven very influential, not only by prescribing valid experimentation, but also for affecting the way we attempt to understand nature [ 18 ], for teaching [ 8 , 12 ], reporting, publishing, and otherwise sharing information [ 28 ], for peer review and the awarding of funds by research-supporting agencies [ 29 , 30 ], for medical diagnostics [ 7 ], and even in litigation [ 31 ].

A second dimension of the scientific method: Data-mining–inspired induction

A major shift in biological experimentation occurred with the–omics revolution of the early 21st century. All of a sudden, it became feasible to perform high-throughput experiments that generated thousands of measurements, typically characterizing the expression or abundances of very many—if not all—genes, proteins, metabolites, or other biological quantities in a sample.

The strategy of measuring large numbers of items in a nontargeted fashion is fundamentally different from the traditional scientific method and constitutes a new, second dimension of the scientific method. Instead of hypothesizing and testing whether gene X is up-regulated under some altered condition, the leading question becomes which of the thousands of genes in a sample are up- or down-regulated. This shift in focus elevates the data to the supreme role of revealing novel insights by themselves ( Fig 2 ). As an important, generic advantage over the traditional strategy, this second dimension is free of a researcher’s preconceived notions regarding the molecular mechanisms governing the phenomenon of interest, which are otherwise the key to formulating a hypothesis. The prominent biologists Patrick Brown and David Botstein commented that “the patterns of expression will often suffice to begin de novo discovery of potential gene functions” [ 32 ].

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Data-driven research begins with an untargeted exploration, in which the data speak for themselves. Machine learning extracts patterns from the data, which suggest hypotheses that are to be tested in the lab or computationally.

This data-driven, discovery-generating approach is at once appealing and challenging. On the one hand, very many data are explored simultaneously and essentially without bias. On the other hand, the large datasets supporting this approach create a genuine challenge to understanding and interpreting the experimental results because the thousands of data points, often superimposed with a fair amount of noise, make it difficult to detect meaningful differences between sample and control. This situation can only be addressed with computational methods that first “clean” the data, for instance, through the statistically valid removal of outliers, and then use machine learning to identify statistically significant, distinguishing molecular profiles or signatures. In favorable cases, such signatures point to specific biological pathways, whereas other signatures defy direct explanation but may become the launch pad for follow-up investigations [ 33 ].

Today’s scientists are very familiar with this discovery-driven exploration of “what’s out there” and might consider it a quaint quirk of history that this strategy was at first widely chastised and ridiculed as a “fishing expedition” [ 30 , 34 ]. Strict traditionalists were outraged that rigor was leaving science with the new approach and that sufficient guidelines were unavailable to assure the validity and reproducibility of results [ 10 , 35 , 36 ].

From the view point of philosophy of science, this second dimension of the scientific method uses inductive reasoning and reflects Bacon’s idea that observations can and should dictate the research question to be investigated [ 1 , 7 ]. Allen [ 36 ] forcefully rejected this type of reasoning, stating “the thinking goes, we can now expect computer programs to derive significance, relevance and meaning from chunks of information, be they nucleotide sequences or gene expression profiles… In contrast with this view, many are convinced that no purely logical process can turn observation into understanding.” His conviction goes back to the 18th century philosopher David Hume and again to Popper, who identified as the overriding problem with inductive reasoning that it can never truly reveal causality, even if a phenomenon is observed time and again [ 16 , 17 , 37 , 38 ]. No number of observations, even if they always have the same result, can guard against an exception that would violate the generality of a law inferred from these observations [ 1 , 35 ]. Worse, Popper argued, through inference by induction, we cannot even know the probability of something being true [ 10 , 17 , 36 ].

Others argued that data-driven and hypothesis-driven research actually do not differ all that much in principle, as long as there is cycling between developing new ideas and testing them with care [ 27 ]. In fact, Kell and Oliver [ 34 ] maintained that the exclusive acceptance of hypothesis-driven programs misrepresents the complexities of biological knowledge generation. Similarly refuting the prominent rule of deduction, Platt [ 26 ] and Beard and Kushmerick [ 27 ] argued that repeated inductive reasoning, called strong inference, corresponds to a logically sound decision tree of disproving or refining hypotheses that can rapidly yield firm conclusions; nonetheless, Platt had to admit that inductive inference is not as certain as deduction, because it projects into the unknown. Lander compared the task of obtaining causality by induction to the problem of inferring the design of a microprocessor from input-output readings, which in a strict sense is impossible, because the microprocessor could be arbitrarily complicated; even so, inference often leads to novel insights and therefore is valuable [ 39 ].

An interesting special case of almost pure inductive reasoning is epidemiology, where hypothesis-driven reasoning is rare and instead, the fundamental question is whether data-based evidence is sufficient to associate health risks with specific causes [ 31 , 34 ].

Recent advances in machine learning and “big-data” mining have driven the use of inductive reasoning to unprecedented heights. As an example, machine learning can greatly assist in the discovery of patterns, for instance, in biological sequences [ 40 ]. Going a step further, a pithy article by Andersen [ 41 ] proffered that we may not need to look for causality or mechanistic explanations anymore if we just have enough correlation: “With enough data, the numbers speak for themselves, correlation replaces causation, and science can advance even without coherent models or unified theories.”

Of course, the proposal to abandon the quest for causality caused pushback on philosophical as well as mathematical grounds. Allen [ 10 , 35 ] considered the idea “absurd” that data analysis could enhance understanding in the absence of a hypothesis. He felt confident “that even the formidable combination of computing power with ease of access to data cannot produce a qualitative shift in the way that we do science: the making of hypotheses remains an indispensable component in the growth of knowledge” [ 36 ]. Succi and Coveney [ 42 ] refuted the “most extravagant claims” of big-data proponents very differently, namely by analyzing the theories on which machine learning is founded. They contrasted the assumptions underlying these theories, such as the law of large numbers, with the mathematical reality of complex biological systems. Specifically, they carefully identified genuine features of these systems, such as nonlinearities, nonlocality of effects, fractal aspects, and high dimensionality, and argued that they fundamentally violate some of the statistical assumptions implicitly underlying big-data analysis, like independence of events. They concluded that these discrepancies “may lead to false expectations and, at their nadir, even to dangerous social, economical and political manipulation.” To ameliorate the situation, the field of big-data analysis would need new strong theorems characterizing the validity of its methods and the numbers of data required for obtaining reliable insights. Succi and Coveney go as far as stating that too many data are just as bad as insufficient data [ 42 ].

While philosophical doubts regarding inductive methods will always persist, one cannot deny that -omics-based, high-throughput studies, combined with machine learning and big-data analysis, have been very successful [ 43 ]. Yes, induction cannot truly reveal general laws, no matter how large the datasets, but they do provide insights that are very different from what science had offered before and may at least suggest novel patterns, trends, or principles. As a case in point, if many transcriptomic studies indicate that a particular gene set is involved in certain classes of phenomena, there is probably some truth to the observation, even though it is not mathematically provable. Kepler’s laws of astronomy were arguably derived solely from inductive reasoning [ 34 ].

Notwithstanding the opposing views on inductive methods, successful strategies shape how we think about science. Thus, to take advantage of all experimental options while ensuring quality of research, we must not allow that “anything goes” but instead identify and characterize standard operating procedures and controls that render this emerging scientific method valid and reproducible. A laudable step in this direction was the wide acceptance of “minimum information about a microarray experiment” (MIAME) standards for microarray experiments [ 44 ].

A third dimension of the scientific method: Allochthonous reasoning

Parallel to the blossoming of molecular biology and the rapid rise in the power and availability of computing in the late 20th century, the use of mathematical and computational models became increasingly recognized as relevant and beneficial for understanding biological phenomena. Indeed, mathematical models eventually achieved cornerstone status in the new field of computational systems biology.

Mathematical modeling has been used as a tool of biological analysis for a long time [ 27 , 45 – 48 ]. Interesting for the discussion here is that the use of mathematical and computational modeling in biology follows a scientific approach that is distinctly different from the traditional and the data-driven methods, because it is distributed over two entirely separate domains of knowledge. One consists of the biological reality of DNA, elephants, and roses, whereas the other is the world of mathematics, which is governed by numbers, symbols, theorems, and abstract work protocols. Because the ways of thinking—and even the languages—are different in these two realms, I suggest calling this type of knowledge acquisition “allochthonous” (literally Greek: in or from a “piece of land different from where one is at home”; one could perhaps translate it into modern lingo as “outside one’s comfort zone”). De facto, most allochthonous reasoning in biology presently refers to mathematics and computing, but one might also consider, for instance, the application of methods from linguistics in the analysis of DNA sequences or proteins [ 49 ].

One could argue that biologists have employed “models” for a long time, for instance, in the form of “model organisms,” cell lines, or in vitro experiments, which more or less faithfully reflect features of the organisms of true interest but are easier to manipulate. However, this type of biological model use is rather different from allochthonous reasoning, as it does not leave the realm of biology and uses the same language and often similar methodologies.

A brief discussion of three experiences from our lab may illustrate the benefits of allochthonous reasoning. (1) In a case study of renal cell carcinoma, a dynamic model was able to explain an observed yet nonintuitive metabolic profile in terms of the enzymatic reaction steps that had been altered during the disease [ 50 ]. (2) A transcriptome analysis had identified several genes as displaying significantly different expression patterns during malaria infection in comparison to the state of health. Considered by themselves and focusing solely on genes coding for specific enzymes of purine metabolism, the findings showed patterns that did not make sense. However, integrating the changes in a dynamic model revealed that purine metabolism globally shifted, in response to malaria, from guanine compounds to adenine, inosine, and hypoxanthine [ 51 ]. (3) Data capturing the dynamics of malaria parasites suggested growth rates that were biologically impossible. Speculation regarding possible explanations led to the hypothesis that many parasite-harboring red blood cells might “hide” from circulation and therewith from detection in the blood stream. While experimental testing of the feasibility of the hypothesis would have been expensive, a dynamic model confirmed that such a concealment mechanism could indeed quantitatively explain the apparently very high growth rates [ 52 ]. In all three cases, the insights gained inductively from computational modeling would have been difficult to obtain purely with experimental laboratory methods. Purely deductive allochthonous reasoning is the ultimate goal of the search for design and operating principles [ 53 – 55 ], which strives to explain why certain structures or functions are employed by nature time and again. An example is a linear metabolic pathway, in which feedback inhibition is essentially always exerted on the first step [ 56 , 57 ]. This generality allows the deduction that a so far unstudied linear pathway is most likely (or even certain to be) inhibited at the first step. Not strictly deductive—but rather abductive—was a study in our lab in which we analyzed time series data with a mathematical model that allowed us to infer the most likely regulatory structure of a metabolic pathway [ 58 , 59 ].

A typical allochthonous investigation begins in the realm of biology with the formulation of a hypothesis ( Fig 3 ). Instead of testing this hypothesis with laboratory experiments, the system encompassing the hypothesis is moved into the realm of mathematics. This move requires two sets of ingredients. One set consists of the simplification and abstraction of the biological system: Any distracting details that seem unrelated to the hypothesis and its context are omitted or represented collectively with other details. This simplification step carries the greatest risk of the entire modeling approach, as omission of seemingly negligible but, in truth, important details can easily lead to wrong results. The second set of ingredients consists of correspondence rules that translate every biological component or process into the language of mathematics [ 60 , 61 ].

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This mathematical and computational approach is distributed over two realms, which are connected by correspondence rules.

Once the system is translated, it has become an entirely mathematical construct that can be analyzed purely with mathematical and computational means. The results of this analysis are also strictly mathematical. They typically consist of values of variables, magnitudes of processes, sensitivity patterns, signs of eigenvalues, or qualitative features like the onset of oscillations or the potential for limit cycles. Correspondence rules are used again to move these results back into the realm of biology. As an example, the mathematical result that “two eigenvalues have positive real parts” does not make much sense to many biologists, whereas the interpretation that “the system is not stable at the steady state in question” is readily explained. New biological insights may lead to new hypotheses, which are tested either by experiments or by returning once more to the realm of mathematics. The model design, diagnosis, refinements, and validation consist of several phases, which have been discussed widely in the biomathematical literature. Importantly, each iteration of a typical modeling analysis consists of a move from the biological to the mathematical realm and back.

The reasoning within the realm of mathematics is often deductive, in the form of an Aristotelian syllogism, such as the well-known “All men are mortal; Socrates is a man; therefore, Socrates is mortal.” However, the reasoning may also be inductive, as it is the case with large-scale Monte-Carlo simulations that generate arbitrarily many “observations,” although they cannot reveal universal principles or theorems. An example is a simulation randomly drawing numbers in an attempt to show that every real number has an inverse. The simulation will always attest to this hypothesis but fail to discover the truth because it will never randomly draw 0. Generically, computational models may be considered sets of hypotheses, formulated as equations or as algorithms that reflect our perception of a complex system [ 27 ].

Impact of the multidimensional scientific method on learning

Almost all we know in biology has come from observation, experimentation, and interpretation. The traditional scientific method not only offered clear guidance for this knowledge gathering, but it also fundamentally shaped the way we think about the exploration of nature. When presented with a new research question, scientists were trained to think immediately in terms of hypotheses and alternatives, pondering the best feasible ways of testing them, and designing in their minds strong controls that would limit the effects of known or unknown confounders. Shaped by the rigidity of this ever-repeating process, our thinking became trained to move forward one well-planned step at a time. This modus operandi was rigid and exact. It also minimized the erroneous pursuit of long speculative lines of thought, because every step required testing before a new hypothesis was formed. While effective, the process was also very slow and driven by ingenuity—as well as bias—on the scientist’s part. This bias was sometimes a hindrance to necessary paradigm shifts [ 22 ].

High-throughput data generation, big-data analysis, and mathematical-computational modeling changed all that within a few decades. In particular, the acceptance of inductive principles and of the allochthonous use of nonbiological strategies to answer biological questions created an unprecedented mix of successes and chaos. To the horror of traditionalists, the importance of hypotheses became minimized, and the suggestion spread that the data would speak for themselves [ 36 ]. Importantly, within this fog of “anything goes,” the fundamental question arose how to determine whether an experiment was valid.

Because agreed-upon operating procedures affect research progress and interpretation, thinking, teaching, and sharing of results, this question requires a deconvolution of scientific strategies. Here I proffer that the single scientific method of the past should be expanded toward a vector space of scientific methods, with spanning vectors that correspond to different dimensions of the scientific method ( Fig 4 ).

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The traditional hypothesis-based deductive scientific method is expanded into a 3D space that allows for synergistic blends of methods that include data-mining–inspired, inductive knowledge acquisition, and mathematical model-based, allochthonous reasoning.

Obviously, all three dimensions have their advantages and drawbacks. The traditional, hypothesis-driven deductive method is philosophically “clean,” except that it is confounded by preconceptions and assumptions. The data-mining–inspired inductive method cannot offer universal truths but helps us explore very large spaces of factors that contribute to a phenomenon. Allochthonous, model-based reasoning can be performed mentally, with paper and pencil, through rigorous analysis, or with a host of computational methods that are precise and disprovable [ 27 ]. At the same time, they are incomparable faster, cheaper, and much more comprehensive than experiments in molecular biology. This reduction in cost and time, and the increase in coverage, may eventually have far-reaching consequences, as we can already fathom from much of modern physics.

Due to its long history, the traditional dimension of the scientific method is supported by clear and very strong standard operating procedures. Similarly, strong procedures need to be developed for the other two dimensions. The MIAME rules for microarray analysis provide an excellent example [ 44 ]. On the mathematical modeling front, no such rules are generally accepted yet, but trends toward them seem to emerge at the horizon. For instance, it seems to be becoming common practice to include sensitivity analyses in typical modeling studies and to assess the identifiability or sloppiness of ensembles of parameter combinations that fit a given dataset well [ 62 , 63 ].

From a philosophical point of view, it seems unlikely that objections against inductive reasoning will disappear. However, instead of pitting hypothesis-based deductive reasoning against inductivism, it seems more beneficial to determine how the different methods can be synergistically blended ( cf . [ 18 , 27 , 34 , 42 ]) as linear combinations of the three vectors of knowledge acquisition ( Fig 4 ). It is at this point unclear to what degree the identified three dimensions are truly independent of each other, whether additional dimensions should be added [ 24 ], or whether the different versions could be amalgamated into a single scientific method [ 18 ], especially if it is loosely defined as a form of critical thinking [ 8 ]. Nobel Laureate Percy Bridgman even concluded that “science is what scientists do, and there are as many scientific methods as there are individual scientists” [ 8 , 64 ].

Combinations of the three spanning vectors of the scientific method have been emerging for some time. Many biologists already use inductive high-throughput methods to develop specific hypotheses that are subsequently tested with deductive or further inductive methods [ 34 , 65 ]. In terms of including mathematical modeling, physics and geology have been leading the way for a long time, often by beginning an investigation in theory, before any actual experiment is performed. It will benefit biology to look into this strategy and to develop best practices of allochthonous reasoning.

The blending of methods may take quite different shapes. Early on, Ideker and colleagues [ 65 ] proposed an integrated experimental approach for pathway analysis that offered a glimpse of new experimental strategies within the space of scientific methods. In a similar vein, Covert and colleagues [ 66 ] included computational methods into such an integrated approach. Additional examples of blended analyses in systems biology can be seen in other works, such as [ 43 , 67 – 73 ]. Generically, it is often beneficial to start with big data, determine patterns in associations and correlations, then switch to the mathematical realm in order to filter out spurious correlations in a high-throughput fashion. If this procedure is executed in an iterative manner, the “surviving” associations have an increased level of confidence and are good candidates for further experimental or computational testing (personal communication from S. Chandrasekaran).

If each component of a blended scientific method follows strict, commonly agreed guidelines, “linear combinations” within the 3D space can also be checked objectively, per deconvolution. In addition, guidelines for synergistic blends of component procedures should be developed. If we carefully monitor such blends, time will presumably indicate which method is best for which task and how the different approaches optimally inform each other. For instance, it will be interesting to study whether there is an optimal sequence of experiments along the three axes for a particular class of tasks. Big-data analysis together with inductive reasoning might be optimal for creating initial hypotheses and possibly refuting wrong speculations (“we had thought this gene would be involved, but apparently it isn’t”). If the logic of an emerging hypotheses can be tested with mathematical and computational tools, it will almost certainly be faster and cheaper than an immediate launch into wet-lab experimentation. It is also likely that mathematical reasoning will be able to refute some apparently feasible hypothesis and suggest amendments. Ultimately, the “surviving” hypotheses must still be tested for validity through conventional experiments. Deconvolving current practices and optimizing the combination of methods within the 3D or higher-dimensional space of scientific methods will likely result in better planning of experiments and in synergistic blends of approaches that have the potential capacity of addressing some of the grand challenges in biology.

Acknowledgments

The author is very grateful to Dr. Sriram Chandrasekaran and Ms. Carla Kumbale for superb suggestions and invaluable feedback.

Funding Statement

This work was supported in part by grants from the National Science Foundation ( https://www.nsf.gov/div/index.jsp?div=MCB ) grant NSF-MCB-1517588 (PI: EOV), NSF-MCB-1615373 (PI: Diana Downs) and the National Institute of Environmental Health Sciences ( https://www.niehs.nih.gov/ ) grant NIH-2P30ES019776-05 (PI: Carmen Marsit). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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Developing a Hypothesis

Rajiv S. Jhangiani; I-Chant A. Chiang; Carrie Cuttler; and Dana C. Leighton

Learning Objectives

  • Distinguish between a theory and a hypothesis.
  • Discover how theories are used to generate hypotheses and how the results of studies can be used to further inform theories.
  • Understand the characteristics of a good hypothesis.

Theories and Hypotheses

Before describing how to develop a hypothesis, it is important to distinguish between a theory and a hypothesis. A  theory  is a coherent explanation or interpretation of one or more phenomena. Although theories can take a variety of forms, one thing they have in common is that they go beyond the phenomena they explain by including variables, structures, processes, functions, or organizing principles that have not been observed directly. Consider, for example, Zajonc’s theory of social facilitation and social inhibition (1965) [1] . He proposed that being watched by others while performing a task creates a general state of physiological arousal, which increases the likelihood of the dominant (most likely) response. So for highly practiced tasks, being watched increases the tendency to make correct responses, but for relatively unpracticed tasks, being watched increases the tendency to make incorrect responses. Notice that this theory—which has come to be called drive theory—provides an explanation of both social facilitation and social inhibition that goes beyond the phenomena themselves by including concepts such as “arousal” and “dominant response,” along with processes such as the effect of arousal on the dominant response.

Outside of science, referring to an idea as a theory often implies that it is untested—perhaps no more than a wild guess. In science, however, the term theory has no such implication. A theory is simply an explanation or interpretation of a set of phenomena. It can be untested, but it can also be extensively tested, well supported, and accepted as an accurate description of the world by the scientific community. The theory of evolution by natural selection, for example, is a theory because it is an explanation of the diversity of life on earth—not because it is untested or unsupported by scientific research. On the contrary, the evidence for this theory is overwhelmingly positive and nearly all scientists accept its basic assumptions as accurate. Similarly, the “germ theory” of disease is a theory because it is an explanation of the origin of various diseases, not because there is any doubt that many diseases are caused by microorganisms that infect the body.

A  hypothesis , on the other hand, is a specific prediction about a new phenomenon that should be observed if a particular theory is accurate. It is an explanation that relies on just a few key concepts. Hypotheses are often specific predictions about what will happen in a particular study. They are developed by considering existing evidence and using reasoning to infer what will happen in the specific context of interest. Hypotheses are often but not always derived from theories. So a hypothesis is often a prediction based on a theory but some hypotheses are a-theoretical and only after a set of observations have been made, is a theory developed. This is because theories are broad in nature and they explain larger bodies of data. So if our research question is really original then we may need to collect some data and make some observations before we can develop a broader theory.

Theories and hypotheses always have this  if-then  relationship. “ If   drive theory is correct,  then  cockroaches should run through a straight runway faster, and a branching runway more slowly, when other cockroaches are present.” Although hypotheses are usually expressed as statements, they can always be rephrased as questions. “Do cockroaches run through a straight runway faster when other cockroaches are present?” Thus deriving hypotheses from theories is an excellent way of generating interesting research questions.

But how do researchers derive hypotheses from theories? One way is to generate a research question using the techniques discussed in this chapter  and then ask whether any theory implies an answer to that question. For example, you might wonder whether expressive writing about positive experiences improves health as much as expressive writing about traumatic experiences. Although this  question  is an interesting one  on its own, you might then ask whether the habituation theory—the idea that expressive writing causes people to habituate to negative thoughts and feelings—implies an answer. In this case, it seems clear that if the habituation theory is correct, then expressive writing about positive experiences should not be effective because it would not cause people to habituate to negative thoughts and feelings. A second way to derive hypotheses from theories is to focus on some component of the theory that has not yet been directly observed. For example, a researcher could focus on the process of habituation—perhaps hypothesizing that people should show fewer signs of emotional distress with each new writing session.

Among the very best hypotheses are those that distinguish between competing theories. For example, Norbert Schwarz and his colleagues considered two theories of how people make judgments about themselves, such as how assertive they are (Schwarz et al., 1991) [2] . Both theories held that such judgments are based on relevant examples that people bring to mind. However, one theory was that people base their judgments on the  number  of examples they bring to mind and the other was that people base their judgments on how  easily  they bring those examples to mind. To test these theories, the researchers asked people to recall either six times when they were assertive (which is easy for most people) or 12 times (which is difficult for most people). Then they asked them to judge their own assertiveness. Note that the number-of-examples theory implies that people who recalled 12 examples should judge themselves to be more assertive because they recalled more examples, but the ease-of-examples theory implies that participants who recalled six examples should judge themselves as more assertive because recalling the examples was easier. Thus the two theories made opposite predictions so that only one of the predictions could be confirmed. The surprising result was that participants who recalled fewer examples judged themselves to be more assertive—providing particularly convincing evidence in favor of the ease-of-retrieval theory over the number-of-examples theory.

Theory Testing

The primary way that scientific researchers use theories is sometimes called the hypothetico-deductive method  (although this term is much more likely to be used by philosophers of science than by scientists themselves). Researchers begin with a set of phenomena and either construct a theory to explain or interpret them or choose an existing theory to work with. They then make a prediction about some new phenomenon that should be observed if the theory is correct. Again, this prediction is called a hypothesis. The researchers then conduct an empirical study to test the hypothesis. Finally, they reevaluate the theory in light of the new results and revise it if necessary. This process is usually conceptualized as a cycle because the researchers can then derive a new hypothesis from the revised theory, conduct a new empirical study to test the hypothesis, and so on. As  Figure 2.3  shows, this approach meshes nicely with the model of scientific research in psychology presented earlier in the textbook—creating a more detailed model of “theoretically motivated” or “theory-driven” research.

formulating a hypothesis scientific method

As an example, let us consider Zajonc’s research on social facilitation and inhibition. He started with a somewhat contradictory pattern of results from the research literature. He then constructed his drive theory, according to which being watched by others while performing a task causes physiological arousal, which increases an organism’s tendency to make the dominant response. This theory predicts social facilitation for well-learned tasks and social inhibition for poorly learned tasks. He now had a theory that organized previous results in a meaningful way—but he still needed to test it. He hypothesized that if his theory was correct, he should observe that the presence of others improves performance in a simple laboratory task but inhibits performance in a difficult version of the very same laboratory task. To test this hypothesis, one of the studies he conducted used cockroaches as subjects (Zajonc, Heingartner, & Herman, 1969) [3] . The cockroaches ran either down a straight runway (an easy task for a cockroach) or through a cross-shaped maze (a difficult task for a cockroach) to escape into a dark chamber when a light was shined on them. They did this either while alone or in the presence of other cockroaches in clear plastic “audience boxes.” Zajonc found that cockroaches in the straight runway reached their goal more quickly in the presence of other cockroaches, but cockroaches in the cross-shaped maze reached their goal more slowly when they were in the presence of other cockroaches. Thus he confirmed his hypothesis and provided support for his drive theory. (Zajonc also showed that drive theory existed in humans [Zajonc & Sales, 1966] [4] in many other studies afterward).

Incorporating Theory into Your Research

When you write your research report or plan your presentation, be aware that there are two basic ways that researchers usually include theory. The first is to raise a research question, answer that question by conducting a new study, and then offer one or more theories (usually more) to explain or interpret the results. This format works well for applied research questions and for research questions that existing theories do not address. The second way is to describe one or more existing theories, derive a hypothesis from one of those theories, test the hypothesis in a new study, and finally reevaluate the theory. This format works well when there is an existing theory that addresses the research question—especially if the resulting hypothesis is surprising or conflicts with a hypothesis derived from a different theory.

To use theories in your research will not only give you guidance in coming up with experiment ideas and possible projects, but it lends legitimacy to your work. Psychologists have been interested in a variety of human behaviors and have developed many theories along the way. Using established theories will help you break new ground as a researcher, not limit you from developing your own ideas.

Characteristics of a Good Hypothesis

There are three general characteristics of a good hypothesis. First, a good hypothesis must be testable and falsifiable . We must be able to test the hypothesis using the methods of science and if you’ll recall Popper’s falsifiability criterion, it must be possible to gather evidence that will disconfirm the hypothesis if it is indeed false. Second, a good hypothesis must be logical. As described above, hypotheses are more than just a random guess. Hypotheses should be informed by previous theories or observations and logical reasoning. Typically, we begin with a broad and general theory and use  deductive reasoning to generate a more specific hypothesis to test based on that theory. Occasionally, however, when there is no theory to inform our hypothesis, we use  inductive reasoning  which involves using specific observations or research findings to form a more general hypothesis. Finally, the hypothesis should be positive. That is, the hypothesis should make a positive statement about the existence of a relationship or effect, rather than a statement that a relationship or effect does not exist. As scientists, we don’t set out to show that relationships do not exist or that effects do not occur so our hypotheses should not be worded in a way to suggest that an effect or relationship does not exist. The nature of science is to assume that something does not exist and then seek to find evidence to prove this wrong, to show that it really does exist. That may seem backward to you but that is the nature of the scientific method. The underlying reason for this is beyond the scope of this chapter but it has to do with statistical theory.

  • Zajonc, R. B. (1965). Social facilitation.  Science, 149 , 269–274 ↵
  • Schwarz, N., Bless, H., Strack, F., Klumpp, G., Rittenauer-Schatka, H., & Simons, A. (1991). Ease of retrieval as information: Another look at the availability heuristic.  Journal of Personality and Social Psychology, 61 , 195–202. ↵
  • Zajonc, R. B., Heingartner, A., & Herman, E. M. (1969). Social enhancement and impairment of performance in the cockroach.  Journal of Personality and Social Psychology, 13 , 83–92. ↵
  • Zajonc, R.B. & Sales, S.M. (1966). Social facilitation of dominant and subordinate responses. Journal of Experimental Social Psychology, 2 , 160-168. ↵

A coherent explanation or interpretation of one or more phenomena.

A specific prediction about a new phenomenon that should be observed if a particular theory is accurate.

A cyclical process of theory development, starting with an observed phenomenon, then developing or using a theory to make a specific prediction of what should happen if that theory is correct, testing that prediction, refining the theory in light of the findings, and using that refined theory to develop new hypotheses, and so on.

The ability to test the hypothesis using the methods of science and the possibility to gather evidence that will disconfirm the hypothesis if it is indeed false.

Developing a Hypothesis Copyright © 2022 by Rajiv S. Jhangiani; I-Chant A. Chiang; Carrie Cuttler; and Dana C. Leighton is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License , except where otherwise noted.

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Definition of a Hypothesis

What it is and how it's used in sociology

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  • Archaeology

A hypothesis is a prediction of what will be found at the outcome of a research project and is typically focused on the relationship between two different variables studied in the research. It is usually based on both theoretical expectations about how things work and already existing scientific evidence.

Within social science, a hypothesis can take two forms. It can predict that there is no relationship between two variables, in which case it is a null hypothesis . Or, it can predict the existence of a relationship between variables, which is known as an alternative hypothesis.

In either case, the variable that is thought to either affect or not affect the outcome is known as the independent variable, and the variable that is thought to either be affected or not is the dependent variable.

Researchers seek to determine whether or not their hypothesis, or hypotheses if they have more than one, will prove true. Sometimes they do, and sometimes they do not. Either way, the research is considered successful if one can conclude whether or not a hypothesis is true. 

Null Hypothesis

A researcher has a null hypothesis when she or he believes, based on theory and existing scientific evidence, that there will not be a relationship between two variables. For example, when examining what factors influence a person's highest level of education within the U.S., a researcher might expect that place of birth, number of siblings, and religion would not have an impact on the level of education. This would mean the researcher has stated three null hypotheses.

Alternative Hypothesis

Taking the same example, a researcher might expect that the economic class and educational attainment of one's parents, and the race of the person in question are likely to have an effect on one's educational attainment. Existing evidence and social theories that recognize the connections between wealth and cultural resources , and how race affects access to rights and resources in the U.S. , would suggest that both economic class and educational attainment of the one's parents would have a positive effect on educational attainment. In this case, economic class and educational attainment of one's parents are independent variables, and one's educational attainment is the dependent variable—it is hypothesized to be dependent on the other two.

Conversely, an informed researcher would expect that being a race other than white in the U.S. is likely to have a negative impact on a person's educational attainment. This would be characterized as a negative relationship, wherein being a person of color has a negative effect on one's educational attainment. In reality, this hypothesis proves true, with the exception of Asian Americans , who go to college at a higher rate than whites do. However, Blacks and Hispanics and Latinos are far less likely than whites and Asian Americans to go to college.

Formulating a Hypothesis

Formulating a hypothesis can take place at the very beginning of a research project , or after a bit of research has already been done. Sometimes a researcher knows right from the start which variables she is interested in studying, and she may already have a hunch about their relationships. Other times, a researcher may have an interest in ​a particular topic, trend, or phenomenon, but he may not know enough about it to identify variables or formulate a hypothesis.

Whenever a hypothesis is formulated, the most important thing is to be precise about what one's variables are, what the nature of the relationship between them might be, and how one can go about conducting a study of them.

Updated by Nicki Lisa Cole, Ph.D

  • What Is a Hypothesis? (Science)
  • Understanding Path Analysis
  • Null Hypothesis Examples
  • What Are the Elements of a Good Hypothesis?
  • What 'Fail to Reject' Means in a Hypothesis Test
  • How Intervening Variables Work in Sociology
  • Null Hypothesis Definition and Examples
  • Understanding Simple vs Controlled Experiments
  • Scientific Method Vocabulary Terms
  • Null Hypothesis and Alternative Hypothesis
  • Six Steps of the Scientific Method
  • What Are Examples of a Hypothesis?
  • Structural Equation Modeling
  • Scientific Method Flow Chart
  • How To Design a Science Fair Experiment
  • Hypothesis Test for the Difference of Two Population Proportions

Module 2: Sociological Research

The scientific method, learning outcomes.

  • Describe the scientific method as it applies to sociological research
  • Distinguish reliability from validity in a research study
  • Distinguish an independent variable from a dependent variable

When sociologists apply the sociological perspective and begin to ask questions, no topic is off limits. Every aspect of human behavior is a source of possible investigation. Sociologists question the world that humans have created and live in. They notice patterns of behavior as people move through that world. Using sociological methods and systematic research within the framework of the scientific method and a scholarly interpretive perspective, sociologists have discovered workplace patterns that have transformed industries, family patterns that have led to legislative changes, and education patterns that have aided structural changes in classrooms.

Photograph of a full moon

Figure 1. Research provides no evidence that crime rates increase during a full moon.

The “crime rate during a full moon” discussion mentioned earlier put forth a few loosely stated opinions. The good news is we can look at data sets that show us if there is a connection between full moons and crime rates. If there appears to be a trend of increased crime during those times, we should begin to investigate other variables to see whether there is something else that could account for this relationship. If we were to discover that more crime occurs during full moons, this information could inform policing strategies and potentially make cities safer during full moons. Of course, we would be left with more questions! What is it about full moons that lead to increases in crime? Is this true for men and women? Young and old? In cities and in rural areas?

Connecting crime to a full moon might not seem like common sense to the skeptic. What about crime and hot weather? Or crime and holidays? Or crime during natural disasters? Are there more violent crimes in states with less restrictive gun policies? You can see how there are many, many questions that can be asked about any given topic, also how this type of research can be extremely important for informing and shaping public policy.

Sociologists make use of tried and true methods of research, such as experiments, surveys, and field research, but humans and their social interacti ons are so diverse that these examples might seem un-scientific. However, this is exactly why scientific models work for studying human behavior. A scientific process of research establishes parameters that help make sure results are sound. The scientific method involves developing and testing theories about the world based on empirical evidence. It is defined by its com mitment to systematic observation of the empirical world and strives to be objective, critical, skeptical, and logical. It involves a series of prescribed steps that have been established over centuries of scholarship.

The figure shows a flowchart that states the scientific method. One: Ask a Question. Two: Research Existing Sources. Three: Formulate a Hypothesis. Four: Design and Conduct a Study. Five: Draw Conclusions. Six: Report Results.

Figure 2. The scientific method is an essential tool in research.

Results of studies tend to provide people with access to knowledge they did not have before—knowledge of other cultures, knowledge of rituals and beliefs, or knowledge of trends and attitudes. No matter what research approach they use, researchers want to maximize the study’s reliability , which refers to how likely research results are to be replicated if the study is reproduced. If another sociologist follows the same research protocols, will they come up with the same results? If so, then the study is reliable . The more exciting the findings, and the more they challenge prevailing understandings, the more likely it is that other sociologists will try to replicate them.

Researchers also strive for validity , which refers to how well the study measures what it was designed to measure. Returning to the crime rate during a full moon topic, the reliability of a study would reflect how well the results represent the average adult crime rate during a full moon. Validity would ensure that the study’s design accurately examined what it was designed to study and not something else such as one’s perception of criminal activity. If police officers believe there is more criminal activity during a full moon, they might be more likely to see criminal activity and to formalize it by making arrests instead of giving warnings, which would actually create the appearance of increased criminal activity–via documentation–during a full moon.  This evidence would be created even if the amount of criminal activity were no different than on any other night. Thus, what is actually being measured is police officers’ perception of crime, and their subsequent actions during a full moon, rather than criminal activity.

Sociologists can use the scientific method not only to collect but also to interpret and analyze the data. They deliberately apply scientific logic and objectivity. They are interested in—but not attached to—the results. They work outside of their own political or social agendas. This doesn’t mean researchers do not have their own personalities, complete with preferences and opinions. But sociologists deliberately use the scientific method to maintain as much objectivity, focus, and consistency as possible in a particular study. In the end, the scientific method provides a shared basis for discussion and analysis (Merton 1963). Typically, the scientific method starts with these steps—1) ask a question, 2) research existing sources, and 3) formulate a hypothesis.

Ask a Question

computer-generated image of a man with his hand on his chin in a thinking pose, with a question mark behind him.

Figure 3. The scientific process begins with a good question.

The first step of the scientific method is to ask a question, describe a problem, and identify the specific area of interest. The topic should be narrow enough to study within a geography and time frame. “Are societies capable of sustained happiness?” would be too vague. Are married people happier than single people? Are people with children happier than people without children? These questions are more specific, but how is happiness defined and measured?

The question should also be broad enough to have universal merit. “What do personal hygiene habits reveal about the values of college freshman at XYZ College?” would be too narrow so we might want to broaden it to a particular age group (i.e. traditional college students ages 18-22). Also, if you sensed some implicit bias in this question, you would be correct to question whether hygiene, a series of behaviors, should be studied as behaviors or as values (beliefs).

That said, happiness and hygiene are worthy topics to study but must be framed as research questions. As you can probably see, this is a difficult process even for veteran sociologists. Sociologists are careful to define their terms. When forming these basic research questions, sociologists develop an operational definition , that is, they define the concept in terms of the physical or concrete steps it takes to objectively measure it. The operational definition identifies an observable condition of the concept. By operationalizing a variable of the concept, all researchers can collect data methodically in a way that supports the overarching goals of validity and reliability in sociological research.

In a hygiene study, for instance, hygiene could be defined as “personal habits to maintain physical appearance (as opposed to health);” however that might be difficult to measure. Would brushing one’s teeth be considered physical appearance (i.e white teeth) or health (i.e. healthy gums, prevent tooth decay, etc.)? To operationalize hygiene, one must be clear about what constitutes personal hygiene for appearance. A researcher could develop a checklist, for example, of things that are included.

Many times, a research question changes. Perhaps after thinking about hygiene and values, the question changes to “How do differing personal hygiene habits reflect cultural gender role norms?” Thus, the ways in culture shapes something very personal would be the topic of this study. Should a woman shave or not shave her legs? Should a man have a beard? Some facial hair? No facial hair? What about nail care for women? For men? 

Watch this video to learn more about the importance of using the scientific method in sociology.

Research Existing Sources

The next step researchers undertake is to conduct background research through a literature review , which is a review of any existing similar or related studies. A visit to the library or a thorough online search of research databases will uncover existing research about the topic of study. This step helps researchers gain a broad understanding of work previously conducted on the topic at hand and enables them to position their own research to build on prior knowledge. Researchers—including student researchers—are responsible for correctly citing existing sources they use in a study or that inform their work. While it is fine to borrow previously published material (as long as it enhances a unique viewpoint), it must be referenced properly and never plagiarized. This step might also prompt the researcher to revisit their research question!

To study hygiene and its value in a particular society, a researcher might sort through existing research and unearth studies about child-rearing, vanity, obsessive-compulsive behaviors, and cultural attitudes toward beauty. It’s important to sift through this information and determine what is relevant. Using existing sources educates researchers and helps refine and improve studies’ designs.

Formulate a Hypothesis

People commonly try to understand the happenings in their world by finding or creating an explanation for an occurrence, which is what we referred to earlier as common sense. Social scientists may develop a hypothesis for the same reason. A hypothesis is a testable educated guess about predicted outcomes between two or more variables; it’s a possible explanation for specific happenings in the social world and allows for testing to determine whether the explanation holds true in many instances, as well as among various groups or in different places. The hypothesis will often predict how one form of human behavior influences another. The  independent variable s  is the cause of the change or the variable that  in fluences the other variable. The dependent variable is the effect , or variable that is changed. It depends  on the independent variable.

For example, researchers establish one form of human behavior as the independent variable and observe the influence it has on a dependent variable. How does gender (the independent variable) affect rate of income (the dependent variable)? 

How does one’s religion (the independent variable) affect family size (the dependent variable)? How is annual income (the dependent variable) affected by level of education (the independent variable)? It is important to note that we are suggesting relationships or correlations between variables and  not  causation. This is known as  correlation . 

As the table shows, an independent variable is the one that influences the other variable. Rather than being “right,” sociologists are interested in the relationships between variables. If we were to examine the last example, what other variables might come into play? Would we see similar patterns of income for all college-educated people or are there disparities for racial and ethnic minorities? Gender minorities? First, we must move into the next research steps: designing and conducting a study and drawing conclusions. You’ll learn more about these types of research methods in the next section of the course.

Think It Over

Sociology is a broad discipline covering many topics. Think about something that interests you and/or relates to your experience or your life. As a college student, you operate within a social world ripe for research!

  • From competitive sports teams to fraternities or sororities to ROTC to intramural sports and student clubs, there are a plethora of groups on college campuses that would make good topics of study. 
  • The proverbial college experience is different based on one’s statuses, particularly minority statuses, such as race, ethnicity, gender, sexual orientation, religion, and social class. 
  • Consider your college’s relationship to the surrounding community and that community’s relationship with the state and/or country. 

If you were to formulate a research question a nd do some preliminary research on these topics, you would likely find that there have been sociological studies conducted on many of these topics. Furthermore, you would find statistical information about student groups and participation, student demographics, and community demographics.

What value does this type of research have for understanding individuals, groups, and communities?

Contribute!

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Chemistry LibreTexts

1.2: Using the Scientific Method

  • Last updated
  • Save as PDF
  • Page ID 85132

  • Elizabeth Gordon
  • Furman University

Learning Objectives

  • To identify the components of the scientific method
  • Classify measurements as being quantitative or qualitative.
  • Evaluate science in the media.

The Scientific Method

Scientists search for answers to questions and solutions to problems by using a procedure called the scientific method. This procedure consists of making observations, formulating hypotheses, and designing experiments, which in turn lead to additional observations, hypotheses, and experiments in repeated cycles (Figure \(\PageIndex{1}\)).

The steps of the scientific method are 1. observation 2. hypothesis 3. experiment which can lead to a law, back to step 1, or to becoming a theory. Theories are further tested by experiments and modified as needed.

Step 1: Make observations

Observations can be qualitative or quantitative. Qualitative observations describe properties or occurrences in ways that do not rely on numbers. Examples of qualitative observations include the following: the outside air temperature is cooler during the winter season, table salt is a crystalline solid, sulfur crystals are yellow, and dissolving a penny in dilute nitric acid forms a blue solution and a brown gas. Quantitative observations are measurements, which by definition consist of both a number and a unit. Examples of quantitative observations include the following: the melting point of crystalline sulfur is 115.21° Celsius, and 35.9 grams of table salt—whose chemical name is sodium chloride—dissolves in 100 grams of water at 20° Celsius. For the question of the dinosaurs’ extinction, the initial observation was quantitative: iridium concentrations in sediments dating to 66 million years ago were 20–160 times higher than normal.

Step 2: Formulate a hypothesis

After deciding to learn more about an observation or a set of observations, scientists generally begin an investigation by forming a hypothesis, a tentative explanation for the observation(s). The hypothesis may not be correct, but it puts the scientist’s understanding of the system being studied into a form that can be tested. For example, the observation that we experience alternating periods of light and darkness corresponding to observed movements of the sun, moon, clouds, and shadows is consistent with either of two hypotheses:

  • Earth rotates on its axis every 24 hours, alternately exposing one side to the sun, or
  • the sun revolves around Earth every 24 hours.

Suitable experiments can be designed to choose between these two alternatives. For the disappearance of the dinosaurs, the hypothesis was that the impact of a large extraterrestrial object caused their extinction. Unfortunately (or perhaps, fortunately), this hypothesis does not lend itself to direct testing by any obvious experiment, but scientists can collect additional data that either support or refute it.

Step 3: Design and perform experiments

After a hypothesis has been formed, scientists conduct experiments to test its validity. Experiments are systematic observations or measurements, preferably made under controlled conditions—that is, under conditions in which a single variable changes.

Step 4: Accept or modify the hypothesis

A properly designed and executed experiment enables a scientist to determine whether the original hypothesis is valid. In which case he can proceed to step 5. In other cases, experiments often demonstrate that the hypothesis is incorrect or that it must be modified thus requiring further experimentation.

Step 5: Development into law and/or theory

More experimental data are then collected and analyzed, at which point a scientist may begin to think that the results are sufficiently reproducible (i.e., dependable) to merit being summarized in law, a verbal or mathematical description of a phenomenon that allows for general predictions. A law simply says what happens; it does not address the question of why.

One example of a law, the law of definite proportions, which was discovered by the French scientist Joseph Proust (1754–1826), states that a chemical substance always contains the same proportions of elements by mass. Thus, sodium chloride (table salt) always contains the same proportion by mass of sodium to chlorine, in this case, 39.34% sodium and 60.66% chlorine by mass, and sucrose (table sugar) is always 42.11% carbon, 6.48% hydrogen, and 51.41% oxygen by mass.

Whereas a law states only what happens, a theory attempts to explain why nature behaves as it does. Laws are unlikely to change greatly over time unless a major experimental error is discovered. In contrast, a theory, by definition, is incomplete and imperfect, evolving with time to explain new facts as they are discovered.

Because scientists can enter the cycle shown in Figure \(\PageIndex{1}\) at any point, the actual application of the scientific method to different topics can take many different forms. For example, a scientist may start with a hypothesis formed by reading about work done by others in the field, rather than by making direct observations.

A Real-World Application of the Scientific Method

In 2007, my husband and I journeyed to China to adopt our daughter. Upon arrival in Beijing, I became violently ill. Due to her visa paperwork, my husband, daughter, and I were required to stay in China for two weeks. Unfortunately, I was ill the entire time. Once the two-week period was up, the three of us flew back to the United States where I continued to be sick. For the next year, I remained ill and lost a total of 30 pounds. The picture below shows me holding my daughter eight months after we returned home from China.

Baby holding a teddy bear is carried by her mother.

I would like you to attempt to perform the scientific method on my situation described above. List the steps of the scientific method along with some plausible explanations. * Please have this ready to discuss in class .*

Exercise \(\PageIndex{1}\)

Classify each statement as a law, a theory, an experiment, a hypothesis, a qualitative observation, or a quantitative observation.

  • Ice always floats on liquid water.
  • Birds evolved from dinosaurs.
  • According to Albert Einstein, mass X speed of light = energy
  • When 10 g of ice was added to 100 mL of water at 25°C, the temperature of the water decreased to 15.5°C after the ice melted.
  • The ingredients of Ivory soap were analyzed to see whether it really is 99.44% pure, as advertised.
  • This is a general statement of a relationship between the properties of liquid and solid water, so it is a law.
  • This is an educated guess regarding the origin of birds, so it is a hypothesis.
  • This is a theory that explains an explanation of events and can be disproven at any time.
  • The temperature is measured before and after a change is made in a system, so these are quantitative observations.
  • This is an analysis designed to test a hypothesis (in this case, the manufacturer’s claim of purity), so it is an experiment.

Exercise \(\PageIndex{2}\)

  • Measured amounts of acid were added to a Rolaids tablet to see whether it really “consumes 47 times its weight in excess stomach acid.”
  • Heat always flows from hot objects to cooler ones, not in the opposite direction.
  • The universe was formed by a massive explosion that propelled matter into a vacuum.
  • Michael Jordan is the greatest pure shooter ever to play professional basketball.
  • Limestone is relatively insoluble in water but dissolves readily in dilute acid with the evolution of a gas.
  • Gas mixtures that contain more than 4% hydrogen in air are potentially explosive.

qualitative observation

quantitative observation

Evaluating Science in the Media

formulating a hypothesis scientific method

Contributors and Attributions

Elizabeth R. Gordon (Furman University)

  • Hayden Cox (Furman University)

The Scientific Method

flowchart of the scientific method

Occam's Razor : If there are several possible explanations, accept the simplest one (the one that makes the fewest assumptions).

IMAGES

  1. PPT

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  2. 2.1C: Formulating the Hypothesis

    formulating a hypothesis scientific method

  3. What Is The Scientific Method and How Does It Work?

    formulating a hypothesis scientific method

  4. How to Write a Hypothesis

    formulating a hypothesis scientific method

  5. How to Write a Strong Hypothesis in 6 Simple Steps

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  6. How Do You Formulate A Hypothesis? Hypothesis Testing Assignment Help

    formulating a hypothesis scientific method

VIDEO

  1. Basics of Hypothesis, theory and scientific laws

  2. Formulating Research Question and Hypothesis

  3. How to frame the Hypothesis statement in your Research

  4. In the scientific method, a hypothesis is an a observation b measurement c test d propos

  5. How To Formulate The Hypothesis/What is Hypothesis?

  6. Hypothesis Formulation

COMMENTS

  1. Scientific hypothesis

    The formulation and testing of a hypothesis is part of the scientific method, the approach scientists use when attempting to understand and test ideas about natural phenomena. The generation of a hypothesis frequently is described as a creative process and is based on existing scientific knowledge, intuition, or experience.

  2. How to Write a Strong Hypothesis

    5. Phrase your hypothesis in three ways. To identify the variables, you can write a simple prediction in if…then form. The first part of the sentence states the independent variable and the second part states the dependent variable. If a first-year student starts attending more lectures, then their exam scores will improve.

  3. Formulating Hypotheses for Different Study Designs

    Formulating Hypotheses for Different Study Designs. Generating a testable working hypothesis is the first step towards conducting original research. Such research may prove or disprove the proposed hypothesis. Case reports, case series, online surveys and other observational studies, clinical trials, and narrative reviews help to generate ...

  4. Scientific Hypotheses: Writing, Promoting, and Predicting Implications

    Formulating hypothesis articles first and calling for multicenter and interdisciplinary research can be a solution in such instances, potentially launching influential scientific directions, if not academic disciplines. ... One of the main differences between scientific hypothesis and review articles relates to the volume of supportive ...

  5. What is a Hypothesis

    Definition: Hypothesis is an educated guess or proposed explanation for a phenomenon, based on some initial observations or data. It is a tentative statement that can be tested and potentially proven or disproven through further investigation and experimentation. Hypothesis is often used in scientific research to guide the design of experiments ...

  6. How to Write a Hypothesis: A Step-by-Step Guide

    The scientific method can derive and test predictions as hypotheses. Empirical research can then provide support (or lack thereof) for the hypotheses. Even failure to find support for a hypothesis still represents a valuable contribution to scientific knowledge. ... Keep it focused: One of the common pitfalls in hypothesis formulation is trying ...

  7. How to Write a Great Hypothesis

    The Hypothesis in the Scientific Method . In the scientific method, whether it involves research in psychology, biology, or some other area, a hypothesis represents what the researchers think will happen in an experiment. The scientific method involves the following steps: Forming a question; Performing background research; Creating a hypothesis;

  8. The scientific method (article)

    The scientific method. At the core of biology and other sciences lies a problem-solving approach called the scientific method. The scientific method has five basic steps, plus one feedback step: Make an observation. Ask a question. Form a hypothesis, or testable explanation. Make a prediction based on the hypothesis.

  9. 1.6.2: Using the Scientific Method

    Scientists search for answers to questions and solutions to problems by using a procedure called the scientific method. This procedure consists of making observations, formulating hypotheses, and designing experiments, which in turn lead to additional observations, hypotheses, and experiments in repeated cycles (Figure 1.6.2.1 1.6.2. 1 ).

  10. On the scope of scientific hypotheses

    2. The scientific hypothesis. In this section, we will describe a functional and descriptive role regarding how scientists use hypotheses. Jeong & Kwon [] investigated and summarized the different uses the concept of 'hypothesis' had in philosophical and scientific texts.They identified five meanings: assumption, tentative explanation, tentative cause, tentative law, and prediction.

  11. 2.4C: Formulating the Hypothesis

    A hypothesis is an assumption or suggested explanation about how two or more variables are related. It is a crucial step in the scientific method and, therefore, a vital aspect of all scientific research. There are no definitive guidelines for the production of new hypotheses. The history of science is filled with stories of scientists claiming ...

  12. What Are The Steps Of The Scientific Method?

    The scientific method is a process that includes several steps: First, an observation or question arises about a phenomenon. Then a hypothesis is formulated to explain the phenomenon, which is used to make predictions about other related occurrences or to predict the results of new observations quantitatively. Finally, these predictions are put to the test through experiments or further ...

  13. 1.2.1: The Scientific Method

    The scientific method is a method of investigation involving experimentation and observation to acquire new knowledge, solve problems, and answer questions. The key steps in the scientific method include the following: Step 1: Make observations. Step 2: Formulate a hypothesis. Step 3: Test the hypothesis through experimentation.

  14. Perspective: Dimensions of the scientific method

    The traditional scientific method: Hypothesis-driven deduction. Research is the undisputed core activity defining science. Without research, the advancement of scientific knowledge would come to a screeching halt. ... In contrast to induction, the widely accepted, traditional scientific method is based on formulating and testing hypotheses ...

  15. What Is a Hypothesis? The Scientific Method

    A hypothesis (plural hypotheses) is a proposed explanation for an observation. The definition depends on the subject. In science, a hypothesis is part of the scientific method. It is a prediction or explanation that is tested by an experiment. Observations and experiments may disprove a scientific hypothesis, but can never entirely prove one.

  16. Developing a Hypothesis

    So a hypothesis is often a prediction based on a theory but some hypotheses are a-theoretical and only after a set of observations have been made, is a theory developed. ... That may seem backward to you but that is the nature of the scientific method. The underlying reason for this is beyond the scope of this chapter but it has to do with ...

  17. (PDF) FORMULATING AND TESTING HYPOTHESIS

    The scientific research process is based on some ... If a r esearcher succeeds in formulating a hypothesis, ... This is an important step in the scientific method because this determines ...

  18. What a Hypothesis Is and How to Formulate One

    A hypothesis is a prediction of what will be found at the outcome of a research project and is typically focused on the relationship between two different variables studied in the research. It is usually based on both theoretical expectations about how things work and already existing scientific evidence. Within social science, a hypothesis can ...

  19. How to Formulate a Hypothesis for an Experiment

    Formulas & Definitions for Formulating a Hypothesis for an Experiment. Scientific Method: The Scientific Method is a multi-step method to acquiring knowledge or solving problems through ...

  20. The Scientific Method

    In the end, the scientific method provides a shared basis for discussion and analysis (Merton 1963). Typically, the scientific method starts with these steps—1) ask a question, 2) research existing sources, and 3) formulate a hypothesis. Try It. Ask a Question. Figure 3. The scientific process begins with a good question.

  21. 1.2: Using the Scientific Method

    The Scientific Method. Scientists search for answers to questions and solutions to problems by using a procedure called the scientific method. This procedure consists of making observations, formulating hypotheses, and designing experiments, which in turn lead to additional observations, hypotheses, and experiments in repeated cycles (Figure 1. ...

  22. Scientific Method

    The Scientific Method. 1. State the Problem or Ask a question (Usually when you find something interesting or puzzling) 2. Get Background Information (Ask an expert, do library research) 3. Make Observations (Survey, Measure, Count, Weigh, Look, Listen, Touch, Taste, Smell) 4. Formulate a Hypothesis (Suggest an answer to your question based on ...